CN105580632A

CN105580632A - Grass-cultivated antler-shaped ganoderma lucidum high-yield cultivation method

Info

Publication number: CN105580632A
Application number: CN201410565585.1A
Authority: CN
Inventors: 李威
Original assignee: Individual
Current assignee: Individual
Priority date: 2014-10-22
Filing date: 2014-10-22
Publication date: 2016-05-18

Abstract

The invention discloses a grass-cultivated antler-shaped ganoderma lucidum high-yield cultivation method. The invention relates to a technical field of agricultural cultivation, and especially relates to a cultivation method of grass-cultivated antler-shaped ganoderma lucidum. With the grass-cultivated antler-shaped ganoderma lucidum high-yield cultivation method provided by the invention, grass-cultivated antler-shaped ganoderma lucidum growth speed can be promoted, and grass-cultivated antler-shaped ganoderma lucidum growth needed nutrients are sufficiently supplied. The cultivation technology is characterized in comprising the following steps: 1, the ganoderma lucidum strain which is grass-cultivated antler-shaped ganoderma lucidum strain is cultivated, wherein mycelial growth temperature is 12-36 DEG C, and an optimal temperature is 24-30 DEG C; 2, bagging is carried out from March 10th; and sterilization is carried out, wherein plastic tube specifications are 15cm*60cm, and thickness is 0.005-0.006cm; 3, when mycelium cultivation inoculation is finished, mycelium cultivation is carried out immediately; and 4, fruiting management is carried out, wherein cultivation is carried out for 1.5-2 months under an appropriate temperature, and the mycelia can penetrate the plastic tubes; and fruiting body cultivation is carried out instead of mycelium cultivation.

Description

Bacterium grass Ganoderma lucidum high-yield culture technique

Technical field

The invention belongs to agricultural planting technique field, especially relate to a kind of implantation methods of bacterium grass Ganoderma lucidum.

Background technology

Bacterium grass Ganoderma lucidum is a kind of medicinal material, and basidiocarps is annual, has handle, suberin. Cap semicircle or nearly cochlear, surface red brown, is puce sometimes, has paint-like gloss. The high warm nature mushroom of Ganoderma all can be grown between 15～35 DEG C, and thermophilic is 25～30 DEG C. Fructification all can be grown in the scope of 10～32 DEG C, but the optimum temperature of the differentiation of former base and fruit body development is 25～28 DEG C. Lower than 25 DEG C, sporophore growth is slow, and cot color and luster is also poor; Higher than 35 DEG C, fructification can be dead. The humidity that glossy ganoderma growth needs is higher. Along with the increase of day and night temperature, mycelia is covered with the time lengthening of bag. Glossy ganoderma is aerobic property fungi, in its whole growth and development process, all needs ozone. Especially the sporophore growth stage of development, more responsive to carbon dioxide. In the time that Carbon Dioxide in Air content increases to 0.1%, fructification just can not parachute-opening, grows up to rangiferoid, and when content reaches 1%, fruit body development is abnormal, without any tissue differentiation, forms deformity. Glossy ganoderma is very responsive to light in growth and development process, and light has obvious inhibitory action to mycelial growth, and the berlin black dark condition speed of growth is the fastest, and in the time that illumination is increased to 3000 lux, the speed of growth only has the half under full dark condition. Sporophore growth is grown indispensable illumination, and in 1500～5000 luxs, stem, cap growth are rapidly, sturdy, cover thick. Ganoderma lucidum is rarer, and very high to the cultivation technique requirement of Ganoderma lucidum, existing Ganoderma lucidum cultivation technique is not obvious to the growing way facilitation of Ganoderma lucidum, and Ganoderma lucidum is grown to required nutriment assurance not.

Summary of the invention

The present invention is exactly for the problems referred to above, and a kind of bacterium grass Ganoderma lucidum speed of growth that promotes is provided, to the bacterium grass Ganoderma lucidum well-off a kind of bacterium grass Ganoderma lucidum high-yield culture technique of required nutriment of growing.

To achieve these goals, the present invention adopts following technical scheme.

Bacterium grass Ganoderma lucidum high-yield culture technique, is characterized in that, comprises the following steps.

One, cultivating ganoderma bacterial strain bacterium grass Ganoderma lucidum bacterial strain, 12 ~ 36 DEG C of mycelial growth temperatures, 24 ~ 30 DEG C of the most applicable temperature, 20 ~ 30 DEG C of fructification differentiation temperature, temperature is during lower than 22 DEG C, and fructification differentiation is undesired.

Two, started bag, sterilizing March 10, the specification of plastic barrel is 15cm × 60cm, thick 0.005 ~ 0.006cm. The bacterium cylinder preparation method's of glossy ganoderma spice, charging, sterilizing are all identical with the method for mushroom bacterium cylinder cultivation. Started inoculation on March 13, it is rare that cave is inoculated than mushroom in inoculation cave, equidistant inoculation 3 ~ 4 caves, same side, and catching up with season as need can be closeer, after inoculation, seals with gummed paper.

Three, after Mycelium culture inoculation finishes, enter Mycelium culture at once. The cultivation of Ganoderma lucidum mycelium will be noted following item: 1. will clean, be dried, ventilate in cultural hypha place. 2. after inoculation, barrel is staggeredly stacked 8 ~ 10 layers with " # " font, four every layer. After inoculation, in 7 ~ 10d, do not move. 3. culturing room's temperature. After inoculation, in one week, temperature is controlled at 18 ~ 20 DEG C, and after one week, temperature is raised to 24 ~ 30 DEG C. 4. culturing room's light management. Before the long saturating compost of mycelia, shading is cultivated, intensified education chamber illumination when mycelia is covered with compost. 5. in the time that growing to 5cm diameter, mycelium germination to carry out acanthopore ventilation.

Four, go out sesame management and cultivate for 1.5 ~ February under thermophilic condition, mycelia can grow barrel, now just transfers fructification to from Mycelium culture and cultivates. Management comprises the management of perforate, row's cylinder moisturizing and temperature, light, ventilation. Bacterium cylinder perforate bacterium cylinder surface mycelium in the time that light is applicable to transfers light yellow or brown film to from white, now will output in time sesame hole. Boring method is to tear inoculation mouthful sealing off, utilizes original inoculation mouth to go out sesame. Row's cylinder humidification is just arranged rapidly bobbin reason after urging flower bud perforate, otherwise tapping dehydration is difficult for forming former base. Before perforate, should dig cloudy furrow, ridge-up bed will be plugged sprung arch bamboo chip, when furrow ditch antecedent soil moisture, spray water, and sleeping the row in cloudy furrow of bacterium cylinder after perforate, and then cover film. Row when cylinder will note the tapping of bacterium cylinder mutually to stagger, sticky being linked togather while avoiding two adjacent glossy ganodermas to grow. Bacterium bed tempertaure is controlled at 20 ~ 30 DEG C.

As a kind of preferred version, sporophore growth management is under suitable temperature, wet condition, and the white knob occurring at tapping after one week of perforate claims sesame flower bud or mushroom flower bud. Zhi Lei is having continued growth under sufficient oxygen condition, just starts to be divided into stem and cap while growing to 2 ~ 3cm, and the cap of glossy ganoderma has obvious phototaxis, and when growth, cap launches towards light source or the stronger face of light. The management of Antler Mythic Fungus sporophore growing period to note following some: 1. reduce ventilation: do not need ventilation every day, can take a breath once by 3 ~ 4d, each time of exchanging gas should not be grown. 2. temperature, humidity: go out sesame canopy temperature and be controlled at 20 ~ 30 DEG C, relative air humidity is controlled at 80% ~ 90%. 3. after the discharge of bacterium cylinder, can not move, if mobile bacterium cylinder position easily forms misshapen mushroom.

Beneficial effect of the present invention.

The present invention, according to Ganoderma lucidum Biological Characteristics of Strain and local climatic characteristic, is producing the aspects such as arrangement in season, cultivation formula, management of producing mushroom, explores the high-yield culture technique of bacterium grass Ganoderma lucidum, obtains remarkable effect. Promote the bacterium grass Ganoderma lucidum speed of growth, because required nutriment that bacterium grass Ganoderma lucidum is grown is in liberal supply, thereby increased the output of bacterium grass Ganoderma lucidum.

Detailed description of the invention

Two, started bag, sterilizing March 10, the bacterium cylinder preparation method of method prepared by bacterium cylinder and mushroom, Uricularia polytricha is basic identical. The specification of plastic barrel is 15cm × 60cm, thick 0.005 ~ 0.006cm. The bacterium cylinder preparation method's of glossy ganoderma spice, charging, sterilizing are all identical with the method for mushroom bacterium cylinder cultivation. Started inoculation on March 13, it is rare that cave is inoculated than mushroom in inoculation cave, equidistant inoculation 3 ~ 4 caves, same side, and catching up with season as need can be closeer, after inoculation, seals with gummed paper.

Claims

1. bacterium grass Ganoderma lucidum high-yield culture technique, is characterized in that, comprises the following steps;

One, cultivating ganoderma bacterial strain bacterium grass Ganoderma lucidum bacterial strain, 12 ~ 36 DEG C of mycelial growth temperatures, 24 ~ 30 DEG C of the most applicable temperature, 20 ~ 30 DEG C of fructification differentiation temperature, temperature is during lower than 22 DEG C, and fructification differentiation is undesired;

Two, started bag, sterilizing March 10, the specification of plastic barrel is 15cm × 60cm, thick 0.005 ~ 0.006cm; The bacterium cylinder preparation method's of glossy ganoderma spice, charging, sterilizing are all identical with the method for mushroom bacterium cylinder cultivation; Started inoculation on March 13, it is rare that cave is inoculated than mushroom in inoculation cave, equidistant inoculation 3 ~ 4 caves, same side, and catching up with season as need can be closeer, after inoculation, seals with gummed paper;

Three, after Mycelium culture inoculation finishes, enter Mycelium culture at once; The cultivation of Ganoderma lucidum mycelium will be noted following item: 1. will clean, be dried, ventilate in cultural hypha place; 2. after inoculation, barrel is staggeredly stacked 8 ~ 10 layers with " # " font, four every layer; After inoculation, in 7 ~ 10d, do not move; 3. culturing room's temperature; After inoculation, in one week, temperature is controlled at 18 ~ 20 DEG C, and after one week, temperature is raised to 24 ~ 30 DEG C; 4. culturing room's light management; Before the long saturating compost of mycelia, shading is cultivated, intensified education chamber illumination when mycelia is covered with compost; 5. in the time that growing to 5cm diameter, mycelium germination to carry out acanthopore ventilation;

Four, go out sesame management and cultivate for 1.5 ~ February under thermophilic condition, mycelia can grow barrel, now just transfers fructification to from Mycelium culture and cultivates; Management comprises the management of perforate, row's cylinder moisturizing and temperature, light, ventilation; Bacterium cylinder perforate bacterium cylinder surface mycelium in the time that light is applicable to transfers light yellow or brown film to from white, now will output in time sesame hole; Boring method is to tear inoculation mouthful sealing off, utilizes original inoculation mouth to go out sesame; Row's cylinder humidification is just arranged rapidly bobbin reason after urging flower bud perforate, otherwise tapping dehydration is difficult for forming former base; Before perforate, should dig cloudy furrow, ridge-up bed will be plugged sprung arch bamboo chip, when furrow ditch antecedent soil moisture, spray water, and sleeping the row in cloudy furrow of bacterium cylinder after perforate, and then cover film; Row when cylinder will note the tapping of bacterium cylinder mutually to stagger, sticky being linked togather while avoiding two adjacent glossy ganodermas to grow; Bacterium bed tempertaure is controlled at 20 ~ 30 DEG C.

2. bacterium grass Ganoderma lucidum high-yield culture technique according to claim 1, is characterized in that: sporophore growth management is under suitable temperature, wet condition, and the white knob occurring at tapping after one week of perforate claims sesame flower bud or mushroom flower bud; Zhi Lei is having continued growth under sufficient oxygen condition, just starts to be divided into stem and cap while growing to 2 ~ 3cm, and the cap of glossy ganoderma has obvious phototaxis, and when growth, cap launches towards light source or the stronger face of light; The management of Antler Mythic Fungus sporophore growing period to note following some: 1. reduce ventilation: do not need ventilation every day, can take a breath once by 3 ~ 4d, each time of exchanging gas should not be grown; 2. temperature, humidity: go out sesame canopy temperature and be controlled at 20 ~ 30 DEG C, relative air humidity is controlled at 80% ~ 90%; 3. after the discharge of bacterium cylinder, can not move, if mobile bacterium cylinder position easily forms misshapen mushroom.