CN107047060A - Justify No. 1 ganoderma lucidum new strains of sesame and its propagation method in river - Google Patents
Justify No. 1 ganoderma lucidum new strains of sesame and its propagation method in river Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H15/00—Fungi; Lichens
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/06—Fungi, e.g. yeasts
- A61K36/07—Basidiomycota, e.g. Cryptococcus
- A61K36/074—Ganoderma
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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Abstract
The invention belongs to microbial agriculture technical field, and in particular to ganoderma lucidum new strains and its propagation method.The technical problem to be solved in the present invention provides one plant of high yield and high quality ganoderma strain.The ganoderma strain Ganoderma lucidum rivers circle sesame No. 1 of the present invention, its preserving number is CGMCC No.13174.River No. 1 culture of circle sesame of the present invention and cultivation period are short, and yield is high；Functional component content is high, is the high-quality raw material of the productions such as health products, medicine, with very considerable economic benefit.
Description
Technical field
The invention belongs to microbial agriculture technical field, and in particular to justify No. 1 ganoderma lucidum new strains of sesame and its propagation method in river.
Background technology
Ganoderma lucidum is the rare medicinal fungi of China's tradition, " celestial grass ", the good name of " seocho " is have, with a variety of physiologically actives
And pharmacological action, it can effectively strengthen human immunological competence, suppress tumour.
The history of China's Ganoderma Lucidum for many years, the ganoderma lucidum product of existing market sale has Wild ganoderma and artificial
Cultivating ganoderma, wherein Ganoderma Lucidum account for major part.Market survey shows that the ganoderma lucidum source of goods enriches in the market, price
It is not high.Ganoderma lucidum high-class product requires more than bacteria cover diameter 10cm, Hou Du≤1cm, it is single do not connect piece, it is cap subcircular, attractive in appearance.Client
It is strict to product requirement, high-class product can sell 35-45 members/kilogram, and small, the lopsided ganoderma lucidum per kilogram price of cap is only more than ten
Member.Therefore, high yield and high quality ganoderma lucidum new strains and breed breeding can further lift the cultivation benefit of ganoderma lucidum.
Analyzed based on more than, the economical character of high-quality, the new varieties of the functional component of high content can effectively widen ganoderma lucidum people
Work cultivation benefit, promotes ganoderma lucidum industry to develop in a healthy way.
The content of the invention
The technical problem to be solved in the present invention provides high yield and high quality ganoderma lucidum new strains.
The technical scheme is that justifying sesame No. 1 there is provided a kind of ganoderma lucidum new strains Ganoderma lucidum rivers.The bacterium
The preserving number of strain is CGMCC No.13174.The preservation time is on October 21st, 2016, and preservation place is Chinese microorganism strain
Preservation administration committee common micro-organisms center.
Specifically, the ITS sequence of the ganoderma strain has the nucleotide sequence as shown in SEQ ID No.1.
It is specific as follows present invention also offers the propagation method of the ganoderma strain：The strain of river circle sesame No. 1 expands numerous production
Need to follow strictly sterile working principle, parent species production PDA culture medium culture, original seed and cultigen production use with cotton seed hulls,
Wood chip is major ingredient, and wheat bran is the culture medium that auxiliary material is constituted；Culture and cultivation condition：15 DEG C~38 DEG C of mycelial growth temperature, goes out sesame
20 DEG C~35 DEG C of temperature.
It is preferred that, 24 DEG C~26 DEG C of mycelial growth temperature.
It is preferred that, go out sesame temperature for 26 DEG C~28 DEG C.
Present invention also offers purposes of the above-mentioned ganoderma strain in medicine or health food is prepared.
The high-quality ganoderma lucidum new strains river circle sesame No. 1 of the present invention has advantages below：
(1) stabilization characteristics of genetics, wide adaptability, yield is high.
(2) sesame shape subcircular, outward appearance uniformity is high, is conducive to large-scale production.
(3) strong adaptability, can be suitable for widely being widely applied plantation.
(4) functional component content is high, and amino acid content is the 4.217% of dry weight, and protein content is the 13.5% of dry weight,
Polyoses content 1.27%, triterpene content 0.87% is the high-quality raw material of the productions such as health products, medicine, with very considerable
Economic benefit.
The new strains that the present invention is provided are accredited as Polyporaceae Ganoderma red sesame kind (Ganoderma lucidum), name
Justify sesame No. 1 for river.The bacterial strain is preserved in：China Committee for Culture Collection of Microorganisms's common micro-organisms center, address is north
No. 3 Institute of Microorganism, Academia Sinica of institute of Jing Shi Chaoyang Districts North Star West Road 1, postcode：100101.Preserving number：CGMCC
No.13174, its biological classification is named as：Ganoderma lucidum.
Brief description of the drawings
Fig. 1, river circle sesame No. 1 hypha form (left side), spore shape (in) and sporophore shape (right side).
Fig. 2, the systematic evolution tree built based on ITS sequence.
Fig. 3,32 ganoderma strain P2 primer electrophoretograms.
Fig. 4,32 ganoderma strain ISSR cluster collection of illustrative plates.
Fig. 5, river circle sesame No. 1 and No. 6 antagonistic effect figures of river sesame.
Fig. 6, river circle sesame No. 1 (Dayi, Chengdu section wood production figure).
Embodiment
The ganoderma strain of the present invention studies the bacterial strain of seed selection for inventor from Fujian Province's Youxi County, is selected through further system
The new lines educated, are identified as Polyporaceae Ganoderma red sesame kind (Ganoderma lucidum), are named as river circle sesame 1
Number.The bacterial strain is preserved in：China Committee for Culture Collection of Microorganisms's common micro-organisms center, preserving number：CGMCC
No.13174, its biological classification is named as：Ganoderma lucidum.
The morphological feature of the bacterial strain is as follows：
1st, morphological feature
Mycelia：It is river circle sesame No. 1 white mycelium, sturdy, there is annesl phenomenon sometimes on inclined-plane；It is wooden after fructification is ripe
Change, cap khaki (conidia powder color and luster), " justifying sesame No. 1 in river " spore is brown, oval；Mycelia is dense white, sturdy；Fructification kidney
Shape is to subcircular, 10~25cm of diameter, 1.5~2.0cm of thickness；Cap is open and flat, surface white yellow at initial stage, russet when ripe, tool
Ring-type rib and radial wrinkle, the back side are golden yellow to faint yellow, bacterial context yellow-white；Stem dark brown is glossy.
2nd, biological characteristics
Nutritional ingredient is originated：River circle sesame No. a kind can PDA culture medium preserve, suitably preserved in 4 DEG C of refrigerator deepfreezes,
Transfer 1 time within every 3 months.The strain of river circle sesame No. 1, which expands numerous production, need to follow strictly sterile working principle, and parent species production is suitable to be used
PDA culture medium culture；Original seed and cultigen production are suitably major ingredient to cotton seed hulls, wood chip, and wheat bran is the culture that auxiliary material is constituted
Base.
Culture and cultivation condition：15 DEG C~38 DEG C of mycelial growth temperature, most suitable 24 DEG C~26 DEG C；Go out sesame temperature 20 DEG C~35
DEG C, most suitable 26 DEG C~28 DEG C.
The screening and identification of the bacterial strain of embodiment 1
(1) source of parent：
In order to enrich Sichuan Province's ganoderma lucidum germ plasm resource, excellent lucidum variety is selected, solves to use kind of a problem in production,
Inventor in 2013 draws from Fujian Province has educated ganoderma strain, and line culture seed selection is carried out by the bacterial strain separating for several times purifying of acquisition
Research.
(2) primary dcreening operation：
The bacterial strain of acquisition is cultivated on a small scale, the sporophore shape of bacterial strain is understood.Screening economical character, preferably (son is real
Body subcircular, 10~25cm of diameter, 1.5~2.0cm of thickness；Cap is open and flat, surface white yellow at initial stage, russet when ripe, has ring
Shape rib and radial wrinkle, the back side are golden yellow to faint yellow, bacterial context yellow-white；It is stem dark brown, glossy) bacterial strain answered
Sieve.
(3) secondary screening：
Expand cultivation scale, understand producing strain, anti-polygamy and the stability etc. of primary dcreening operation.
1 32 ganoderma strain yield of table and polysaccharide data
(32 bacterial strains of table 1 are 32 bacterial strains of corresponding table 2.No. 31 are filtered out with reference to polyoses content height and economical character
Bacterial strain)
(4) bacterial strain authenticity identification：
Foundation《NY/T1730-2009 edible fungus species authenticity identification ISSR methods》, 32 plants of bacterial strains (table 2), are carried out altogether
Bacterial strain (strain) authenticity identification, identification primer used is shown in Table 3.
2 32 ganoderma strain numberings of table
| Numbering | Strain name | Bacterium source | Numbering | Strain name | Bacterium source |
| 1 | SLZ177 | The wild separation of Panzhihua | 17 | SLZ194 | The wild separation of Panzhihua |
| 2 | SLZ178 | The wild separation of Panzhihua | 18 | SLZ195 | The wild separation of Panzhihua |
| 3 | SLZ179 | The wild separation of Panzhihua | 19 | SLZ196 | The wild separation of Panzhihua |
| 4 | SLZ180 | The wild separation of Panzhihua | 20 | SLZ197 | The wild separation of Panzhihua |
| 5 | SLZ181 | The wild separation of Panzhihua | 21 | SLZ198 | The wild separation of Panzhihua |
| 6 | SLZ183 | The wild separation of Panzhihua | 22 | SLZ199 | The wild separation of Panzhihua |
| 7 | SLZ184 | The wild separation of Panzhihua | 23 | SLZ200 | The wild separation of Panzhihua |
| 8 | SLZ185 | The wild separation of Panzhihua | 24 | SLZ201 | The wild separation of Panzhihua |
| 9 | SLZ186 | The wild separation of Panzhihua | 25 | SLZ202 | The wild separation of Panzhihua |
| 10 | SLZ187 | The wild separation of Panzhihua | 26 | SLZ203 | The wild separation of Panzhihua |
| 11 | SLZ188 | The wild separation of Panzhihua | 27 | SLZ204 | The wild separation of Panzhihua |
| 12 | SLZ189 | The wild separation of Panzhihua | 28 | SLZ205 | The wild separation of Panzhihua |
| 13 | SLZ190 | The wild separation of Panzhihua | 29 | ZZMY | Mianyang Zitong |
| 14 | SLZ191 | The wild separation of Panzhihua | 30 | PZNO.1 | The wild separation of Panzhihua |
| 15 | SLZ192 | The wild separation of Panzhihua | 31 | CYZNO.1 | Justify sesame No. 1 in river |
| 16 | SLZ193 | The wild separation of Panzhihua | 32 | CZNO.6 | Sichuan Zhu Zai rivers sesame No. 6 |
3 10 primer sequences of table
| Numbering | Primer | Primer sequence | Renaturation temperature |
| 1 | ISSR807 | AGAGAGAGAGAGAGAGT(SEQ ID No.4) | 49℃ |
| 2 | ISSR810 | GAGAGAGAGAGAGAGAT(SEQ ID No.5) | 47℃ |
| 3 | ISSR811 | GAGAGAGAGAGAGAGAC(SEQ ID No.6) | 49℃ |
| 4 | ISSR816 | CACACACACACACACAT(SEQ ID No.7) | 47℃ |
| 5 | ISSR861 | ACCACCACCACCACCACC(SEQ ID No.8) | 54℃ |
| 6 | ISSR866 | CTCCTCCTCCTCCTCCTC(SEQ ID No.9) | 49℃ |
| 7 | ISSR873 | GACAGACAGACAGACA(SEQ ID No.10) | 49℃ |
| 8 | ISSR878 | GGATGGATGGATGGAT(SEQ ID No.11) | 49℃ |
| 9 | E45846 | ACTTCCCCACAGGTTAACACA(SEQ ID No.12) | 54℃ |
| 10 | E26637 | AGAGAGAGAGAGAGAGG(SEQ ID No.13) | 54℃ |
Finger-print (Fig. 3) result shows that river circle sesame No. 1 is with existing kind and other control strains in molecular biology water
Otherness is there is on flat.It is red sesame that river circle sesame No. 1 is identified with ITS sequence constructing system chadogram (Fig. 2, Fig. 4), and with
There is certain otherness in molecular biology level in existing kind and other control strains.
In order to further verify strain authenticity, set and complete related antagonistic experiment.Foundation《NY/T1845-2010
Edible fungus species distinctiveness identifies Antagonistic reaction》, 32 plants of bacterial strains, carry out bacterial strain (strain) distinctiveness qualification result show, river justify
Sesame No. 1 has otherness with existing kind and the strain of other wild Ganodermas, and this just further demonstrate that No. 1 bacterial strain of sesame is justified in river
It is different from existing ganoderma strain (Fig. 5).
The river circle sesame No. 1 of identification is cultivated, its hypha form (Fig. 1 is left), spore shape (in Fig. 1) and fructification shape
State (Fig. 1 is right).It is observed that form is as follows：Mycelia：It is dense white, sturdy, 4.0~6.0 μm of diameter.Spore：Brown, oval, diameter
8~11.5 μm.Fructification kidney shape is to subcircular, and 10~25cm of diameter, 1.5~2.0cm of thickness, cap is open and flat, and surface is yellow in vain initial stage
Color, russet when ripe, tool ring-type rib and radial wrinkle, the back side are golden yellow to faint yellow, bacterial context yellow-white；Stem is deeply brown
Color is glossy.
Justify sesame No. 1 through specific primer ITS1 (SEQ ID No.2) in river：TCCGTAGGTGAACCTGCGG, ITS4 (SEQ
ID No.3)：TCCTCC GCTTATTGATATGC PCR amplifications, by building chadogram, identify that river circle sesame No. 1 is red sesame
Ganoderma lucidum。
The ITS sequence of SEQ ID No.1 rivers circle sesame No. 1 is following (SEQ ID No.1)：
GGCTTTCGAAGCTACGACGTGGATGTAGCAGGCCTTCCGAGGCATGTGCACGCCCTGCTCATCCACTCTACACCTGT
GCACTTACTGTGGGCTTCAGATTGCGAGGCACGCTCTTTACCGGGCTTGCGGAGCATATCTGTGCCTGCGTTTATCA
CAAACTCTATAAAGTAACAGAATGTGTATTGCGATGTAACACATCTATATACAACTTTCAGCAACGGATCTCTTGGC
TCTCGCATCGATGAAGAACGCAGCGAAATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTG
AACGCACCTTGCGCTCCTTGGTATTCCGAGGAGCATGCCTGTTTGAGTGTCATGAAATCTTCAACCTACAAGCTTTT
GTGGTTTGTAGGCTTGGACTTGGAGGCTTGTCGGCCGTTATCGGTCGGCTCCTCTTAAATGCATTAGCTTGGTTCCT
TGCGGATCGGCTCTCGGTGTGATAATGTCTACGCCGTGACCGTGAAGCGTTTGGCGAGCTTCTAACCGTCTTATAAG
ACAGCTTTATGACCTCTGACCTCAAATCAGGTAGGACTACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAA。
The cultural area contrast test of embodiment 2
For the cultivation promotional value of clear and definite new strains " river justify sesame No. 1 ", according to the Crop breed audit committee of Sichuan Province
《Edible fungus variety approving standard》, continuous two season (i.e. 2 years) carried out the underground regional testing of river circle sesame No. 1 plastic bag cultivation experiment
(hereinafter referred to as area's examination).
For examination reference kind：The kind river sesame No. 6 of upper commerial growing is produced with current Sichuan Province, and justifies sesame 1 as river
Number control.Cultigen with batch making river circle sesame No. 1 and river sesame No. 6 is standby.
Plant formulation：Cotton seed hulls 60%, wood chip 30%, corn flour 4%, wheat bran 5%, gypsum 1%.Water content 65%,
PH is natural.Dry, do not go mouldy for examination planting material.
Cartridge bag：The container containing of experimental cultivar planting material matrix is used as using polyethylene plastic bag plastic barrel bag.Cylinder
Bag specification (length × folding footpath × thickness) is 33cm × 17cm × 0.025cm.
(1) First Year regional testing
Regional testing is carried out with plastic bag cultivation, each 350 bags of variety culture sets 3 repetitions altogether, it is each to repeat 100 bags, indoors
Carry out cultivating sesame management on bedstead.As a result show (table 4)：No. 1 fresh sesame 158.3g of average every bag of production of river circle sesame, compared with river sesame 6
Number volume increase 10.1%.Product quality is higher than NF standard, the river circle work song entity 10~25cm of bacteria cover diameter of sesame 1, lid thickness 1.5
~2.0cm, stem 2~4cm of length, cap is open and flat, surface white yellow at initial stage, russet when ripe, has ring-type rib and radial
Wrinkle, the back side is golden yellow to faint yellow, bacterial context yellow-white；Stem dark brown is glossy.
Justify the regional testing (Jintang Zhao Jiazhen) of sesame No. 1 in the river of table 4
(2) Second Year regional testing
Regional testing is carried out with plastic bag cultivation, each 350 bags of variety culture sets 3 repetitions altogether, it is each to repeat 100 bags, indoors
Cultivate, be managed according to a conventional method on bedstead.As a result show (table 5)：No. 1 fresh sesame 157.7g of average every bag of production of river circle sesame,
Than No. 6 volume increase 11.0% of river sesame.River circle No. 1 product quality of sesame reaches similar breed standard.Fructification is kidney-shaped to subcircular, directly
10~25cm of footpath, 1.5~2.0cm of thickness, cap is open and flat, surface white yellow at initial stage, russet when ripe, tool ring-type rib and radiation
Shape wrinkle, the back side is golden yellow to faint yellow, bacterial context yellow-white；Stem dark brown is glossy.
Justify the regional testing (Dayi, Chengdu) of sesame No. 1 in the river of table 5
The comprehensive agronomy characters such as the maturity period, morphological feature and yield of " justifying sesame No. 1 in river " kind fructification are in areal
Show more consistent under the conditions of identical planting environment；The general effect more one shown in different regions (two region points)
Cause.This shows, the stabilization characteristics of genetics of " justifying sesame No. 1 in river " and the characteristics of with wide adaptability.It is more larger than control amount of increase in production, it is rich
Production property is good.With the value being widely applied in production.
The wooden pilot production of 3 sections of embodiment
It is used for the cultivation situation of section wood production to probe into " justifying sesame No. 1 in river " strain, is carried out on Sichuan Jintang, Dayi and other places
Underground section of wooden pilot production (Fig. 6), " justifying sesame No. 1 in river " resistance is better than river sesame No. 6, and pollution rate is 5%, less than control river sesame 6
Number.The yield of " justifying sesame No. 1 in river " in Jintang is 553kg/ mus, relatively control volume increase 10.5%；The yield of " justifying sesame No. 1 in river " in Dayi
For 556kg/ mus, relatively control volume increase 11.2%.Fructification is kidney-shaped to subcircular, 10~25cm of diameter, thickness 1.5~2.0cm, bacterium
Open and flat, surface white yellow at initial stage, russet when ripe, tool ring-type rib and radial wrinkle are covered, the back side is golden yellow to faint yellow, bacterium
Meat yellow-white；Stem dark brown is glossy.
Result of the test is shown in Table 6.Section wood pilot production can be seen that：" justifying sesame No. 1 in river " is on mycelium growth vigor, pollution rate
River sesame No. 6 is substantially better than, " justifying sesame No. 1 in river " simultaneously has more neat sesame body, while having higher yield.In economical character
And river sesame No. 6 has been above on cultivation benefit.
Justify No. 1 pilot production of sesame in the river of table 6
The attributional analysis of embodiment 4 and medicinal ingredient
The result that commission China Testing Technology Institute determines to fructification component content shows that the amino acid of sesame No. 1 is justified in river
Content is the 4.217% of dry weight, and protein content is the 13.5% of dry weight, polyoses content 1.27%, triterpene content 0.87%.
(《Chinese Pharmacopoeia》Version in 2015, polyoses content must not be less than 0.90%；Triterpene and sterol must not be less than with oleanolic acid
0.50%).Therefore, the standard that circle sesame No. 1 in river complies fully with NF, and functional component content is higher, with higher medicine
With value.
Justify the component content measurement result of sesame No. 1 in the river of table 7
SEQUENCE LISTING
<110>Inst. of Soil Fertilizer, Sichuan Academy of Agriculture Science
<120>Justify No. 1 ganoderma lucidum new strains of sesame and its propagation method in river
<130> A170156K
<160> 13
<170> PatentIn version 3.3
<210> 1
<211> 613
<212> DNA
<213> Ganoderma lucidum
<400> 1
ggctttcgaa gctacgacgt ggatgtagca ggccttccga ggcatgtgca cgccctgctc 60
atccactcta cacctgtgca cttactgtgg gcttcagatt gcgaggcacg ctctttaccg 120
ggcttgcgga gcatatctgt gcctgcgttt atcacaaact ctataaagta acagaatgtg 180
tattgcgatg taacacatct atatacaact ttcagcaacg gatctcttgg ctctcgcatc 240
gatgaagaac gcagcgaaat gcgataagta atgtgaattg cagaattcag tgaatcatcg 300
aatctttgaa cgcaccttgc gctccttggt attccgagga gcatgcctgt ttgagtgtca 360
tgaaatcttc aacctacaag cttttgtggt ttgtaggctt ggacttggag gcttgtcggc 420
cgttatcggt cggctcctct taaatgcatt agcttggttc cttgcggatc ggctctcggt 480
gtgataatgt ctacgccgtg accgtgaagc gtttggcgag cttctaaccg tcttataaga 540
cagctttatg acctctgacc tcaaatcagg taggactacc cgctgaactt aagcatatca 600
ataagcggag gaa 613
<210> 2
<211> 19
<212> DNA
<213> Artificial
<220>
<223>Primer I TS1
<400> 2
tccgtaggtg aacctgcgg 19
<210> 3
<211> 20
<212> DNA
<213> Artificial
<220>
<223>Primer I TS4
<400> 3
tcctccgctt attgatatgc 20
<210> 4
<211> 17
<212> DNA
<213> Artificial
<220>
<223>Primer I SSR807
<400> 4
agagagagag agagagt 17
<210> 5
<211> 17
<212> DNA
<213> Artificial
<220>
<223>Primer I SSR810
<400> 5
gagagagaga gagagat 17
<210> 6
<211> 17
<212> DNA
<213> Artificial
<220>
<223>Primer I SSR811
<400> 6
gagagagaga gagagac 17
<210> 7
<211> 17
<212> DNA
<213> Artificial
<220>
<223>Primer I SSR816
<400> 7
cacacacaca cacacat 17
<210> 8
<211> 18
<212> DNA
<213> Artificial
<220>
<223>Primer I SSR861
<400> 8
accaccacca ccaccacc 18
<210> 9
<211> 18
<212> DNA
<213> Artificial
<220>
<223>Primer I SSR866
<400> 9
ctcctcctcc tcctcctc 18
<210> 10
<211> 16
<212> DNA
<213> Artificial
<220>
<223>Primer I SSR873
<400> 10
gacagacaga cagaca 16
<210> 11
<211> 16
<212> DNA
<213> Artificial
<220>
<223>Primer I SSR878
<400> 11
ggatggatgg atggat 16
<210> 12
<211> 21
<212> DNA
<213> Artificial
<220>
<223>Primer E45846
<400> 12
acttccccac aggttaacac a 21
<210> 13
<211> 17
<212> DNA
<213> Artificial
<220>
<223>Primer E26637
<400> 13
agagagagag agagagg 17
Claims (6)
1. sesame No. 1 is justified in ganoderma strain Ganoderma lucidum rivers, its preserving number is CGMCC No.13174.
2. ganoderma strain as claimed in claim 1, it is characterised in that：The ITS sequence of ganoderma strain has such as SEQ ID No.1
Shown nucleotide sequence.
3. the propagation method of ganoderma strain described in claim 1 or 2, it is characterised in that：It is specific as follows：Justify the strain of sesame No. 1 in river
Sterile working principle need to be followed strictly by expanding numerous production, parent species production PDA culture medium culture, original seed and cultigen production use with
Cotton seed hulls, wood chip are major ingredient, and wheat bran is the culture medium that auxiliary material is constituted；Culture and cultivation condition：Mycelial growth temperature 15 DEG C~38
DEG C, go out 20 DEG C~35 DEG C of sesame temperature.
4. propagation method as claimed in claim 3, it is characterised in that：24 DEG C~26 DEG C of mycelial growth temperature.
5. the propagation method as described in claim 3 or 4, it is characterised in that：Go out sesame temperature for 26 DEG C~28 DEG C.
6. purposes of the ganoderma strain described in claim 1 or 2 in medicine or health food is prepared.
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| CN113767812A (en) * | 2021-09-23 | 2021-12-10 | 山西农业大学山西功能食品研究院 | Ganoderma strain and culture method of ganoderma lucidum fruiting body |
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| CN114214214A (en) * | 2021-12-31 | 2022-03-22 | 泰安市农业科学院(山东省农业科学院泰安市分院) | Ganoderma strain and cross breeding method thereof |
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