CN105555949A - 使用合成信使rna无饲养层衍生人诱导多能干细胞 - Google Patents
使用合成信使rna无饲养层衍生人诱导多能干细胞 Download PDFInfo
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| PCT/US2014/040362 WO2014190361A2 (en) | 2013-05-13 | 2014-05-30 | Feeder-free derivation of human-induced pluripotent stem cells with synthetic messenger rna |
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| EP (2) | EP3904504A1 (enExample) |
| JP (2) | JP6971574B2 (enExample) |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107142244A (zh) * | 2017-07-03 | 2017-09-08 | 广州润虹医药科技股份有限公司 | 培养基及其用途与诱导多能干细胞向间充质干细胞转化的方法 |
| CN110241087A (zh) * | 2012-05-13 | 2019-09-17 | 美国绿阳生物技术及医药公司 | 使用合成的信使rna无饲养细胞地衍生人类诱导性多能干细胞 |
| CN110291190A (zh) * | 2016-12-28 | 2019-09-27 | 京都府公立大学法人 | 骨骼肌细胞及其诱导方法 |
| CN114207118A (zh) * | 2019-05-14 | 2022-03-18 | 阿利夫农场公司 | 多能细胞聚集体及其用途 |
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| US8497124B2 (en) | 2011-12-05 | 2013-07-30 | Factor Bioscience Inc. | Methods and products for reprogramming cells to a less differentiated state |
| DK3260140T3 (da) | 2011-12-05 | 2021-04-19 | Factor Bioscience Inc | Fremgangsmåder og produkter til transficering af celler |
| US10155929B2 (en) * | 2012-05-13 | 2018-12-18 | Allele Biotechnology & Pharmaceuticals, Inc. | Feeder-free derivation of human-induced pluripotent stem cells with synthetic messenger RNA |
| US10119150B2 (en) | 2012-05-13 | 2018-11-06 | Allele Biotechnology & Pharmaceuticals, Inc. | Feeder-free Derivation of human-induced pluripotent stem cells with synthetic messenger RNA |
| CA2876868A1 (en) * | 2012-06-13 | 2013-12-19 | Stemgent, Inc. | Methods of preparing pluripotent stem cells |
| BR122019025681B1 (pt) | 2012-11-01 | 2023-04-18 | Factor Bioscience Inc | Método para inserir uma sequência de ácido nucleico em uma localização segura de um genoma de uma célula |
| WO2015117021A1 (en) | 2014-01-31 | 2015-08-06 | Factor Bioscience Inc. | Methods and products for nucleic acid production and delivery |
| CA2950582C (en) * | 2014-05-30 | 2020-09-22 | Allele Biotechnology And Pharmaceuticals, Inc. | Feeder-free derivation of human-induced pluripotent stem cells with synthetic messenger rna |
| EP3543339A1 (en) | 2015-02-13 | 2019-09-25 | Factor Bioscience Inc. | Nucleic acid products and methods of administration thereof |
| WO2019241569A1 (en) * | 2018-06-16 | 2019-12-19 | Luigi Warren | Reprogramming cells with synthetic messenger rna |
| CN105567642B (zh) * | 2016-02-01 | 2019-07-12 | 中国科学院生物物理研究所 | 一种着色性干皮病人多能干细胞的制备方法 |
| CN109803977B (zh) | 2016-08-17 | 2023-03-17 | 菲克特生物科学股份有限公司 | 核酸产品及其施用方法 |
| CA3043372A1 (en) | 2016-11-16 | 2018-05-24 | Allele Biotechnology & Pharmaceuticals, Inc. | Induction of pancreatic beta cells by stem cell differentiation with rna |
| EP3652200A4 (en) | 2017-07-13 | 2021-04-28 | Allele Biotechnology And Pharmaceuticals, Inc. | INDUCTION OF NEURAL PROGENITOR CELLS, OLIGODENDROCYTE AND OLIGODENDROCYTE PROGENITOR CELLS BY DIFFERENTIATION OF STEM CELLS USING MARKET TRANSCRIPTION FACTORS |
| SG11202008839WA (en) * | 2018-03-13 | 2020-10-29 | Univ Leland Stanford Junior | Transient cellular reprogramming for reversal of cell aging |
| WO2021003462A1 (en) | 2019-07-03 | 2021-01-07 | Factor Bioscience Inc. | Cationic lipids and uses thereof |
| US10501404B1 (en) | 2019-07-30 | 2019-12-10 | Factor Bioscience Inc. | Cationic lipids and transfection methods |
| JP2022104813A (ja) | 2020-12-29 | 2022-07-11 | アイ ピース，インコーポレイテッド | リプログラミング因子を導入された細胞の培養方法 |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102216445A (zh) * | 2008-09-18 | 2011-10-12 | 新加坡科技研究局 | 诱导多能干细胞的标记 |
| US20130302295A1 (en) * | 2012-05-13 | 2013-11-14 | Allele Biotechnology And Pharmaceuticals, Inc. | Feeder-free derivation of human-induced pluripotent stem cells with synthetic messenger rna |
| CN103747805A (zh) * | 2011-04-22 | 2014-04-23 | 普罗森萨科技有限公司 | 用于治疗、延缓和/或预防人类遗传性疾病如强直性肌营养不良症1型（dm1）的新化合物 |
Family Cites Families (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5260432A (en) | 1989-06-22 | 1993-11-09 | Sloan-Kettering Institute For Cancer Research | Human gamma retinoic acid receptor DNA |
| JP2008307007A (ja) * | 2007-06-15 | 2008-12-25 | Bayer Schering Pharma Ag | 出生後のヒト組織由来未分化幹細胞から誘導したヒト多能性幹細胞 |
| EP2881461A1 (en) | 2008-11-21 | 2015-06-10 | Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. | Reprogramming cells toward a pluripotent state |
| WO2010108126A2 (en) | 2009-03-19 | 2010-09-23 | Fate Therapeutics, Inc. | Reprogramming compositions and methods of using the same |
| WO2011016588A1 (en) * | 2009-08-07 | 2011-02-10 | Kyoto University | Method of efficiently establishing induced pluripotent stem cells |
| CN102190731B (zh) * | 2010-03-09 | 2016-01-27 | 中国科学院上海生命科学研究院 | 用人工转录因子诱导产生多能干细胞 |
| WO2011140397A2 (en) * | 2010-05-05 | 2011-11-10 | The Regents Of The University Of California Office Of The President | Stem cell defined media for xeno-free and feeder free conditions and uses thereof |
| WO2011142832A2 (en) | 2010-05-13 | 2011-11-17 | Whitehead Institute For Biomedical Research | Stem cells derived under low oxygen conditions |
| WO2012012708A1 (en) | 2010-07-22 | 2012-01-26 | Regents Of The University Of Minnesota | Induced pluripotent stem cells |
| US8497124B2 (en) | 2011-12-05 | 2013-07-30 | Factor Bioscience Inc. | Methods and products for reprogramming cells to a less differentiated state |
| CA2864702A1 (en) | 2012-02-22 | 2013-08-29 | Amgen Inc. | Autologous mammalian models derived from induced pluripotent stem cells and related methods |
| EP3561054B1 (en) | 2012-05-13 | 2023-07-05 | Allele Biotechnology And Pharmaceuticals, Inc. | Feeder-free derivation of human-induced pluripotent stem cells with synthetic messenger rna |
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- 2014-05-30 EP EP14801588.6A patent/EP2997130A4/en not_active Withdrawn
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2015
- 2015-11-13 MX MX2021005715A patent/MX2021005715A/es unknown
-
2021
- 2021-07-26 JP JP2021121453A patent/JP2021166553A/ja not_active Withdrawn
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102216445A (zh) * | 2008-09-18 | 2011-10-12 | 新加坡科技研究局 | 诱导多能干细胞的标记 |
| CN103747805A (zh) * | 2011-04-22 | 2014-04-23 | 普罗森萨科技有限公司 | 用于治疗、延缓和/或预防人类遗传性疾病如强直性肌营养不良症1型（dm1）的新化合物 |
| US20130302295A1 (en) * | 2012-05-13 | 2013-11-14 | Allele Biotechnology And Pharmaceuticals, Inc. | Feeder-free derivation of human-induced pluripotent stem cells with synthetic messenger rna |
Non-Patent Citations (1)
| Title |
|---|
| KATALIN KARIKÓ等: "Generating the optimal mRNA for therapy: HPLC purification eliminates immune activation and improves translation of nucleoside-modified, protein-encoding mRNA", 《NUCLEIC ACIDS RESEARCH》 * |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110241087A (zh) * | 2012-05-13 | 2019-09-17 | 美国绿阳生物技术及医药公司 | 使用合成的信使rna无饲养细胞地衍生人类诱导性多能干细胞 |
| CN110241087B (zh) * | 2012-05-13 | 2023-02-03 | 美国绿阳生物技术及医药公司 | 使用合成的信使rna无饲养细胞地衍生人类诱导性多能干细胞 |
| CN110291190A (zh) * | 2016-12-28 | 2019-09-27 | 京都府公立大学法人 | 骨骼肌细胞及其诱导方法 |
| CN110291190B (zh) * | 2016-12-28 | 2024-06-04 | 京都府公立大学法人 | 骨骼肌细胞及其诱导方法 |
| CN107142244A (zh) * | 2017-07-03 | 2017-09-08 | 广州润虹医药科技股份有限公司 | 培养基及其用途与诱导多能干细胞向间充质干细胞转化的方法 |
| CN114207118A (zh) * | 2019-05-14 | 2022-03-18 | 阿利夫农场公司 | 多能细胞聚集体及其用途 |
| US12247216B2 (en) | 2019-05-14 | 2025-03-11 | Aleph Farms Ltd. | Pluripotent cell aggregates and use thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| JP2016521541A (ja) | 2016-07-25 |
| WO2014190361A2 (en) | 2014-11-27 |
| MX2021005715A (es) | 2021-07-21 |
| HK1224335A1 (zh) | 2017-08-18 |
| WO2014190361A3 (en) | 2015-07-30 |
| SG10202006695TA (en) | 2020-08-28 |
| EP2997130A2 (en) | 2016-03-23 |
| EP3904504A1 (en) | 2021-11-03 |
| US20130302295A1 (en) | 2013-11-14 |
| HK1222675A1 (zh) | 2017-07-07 |
| JP6971574B2 (ja) | 2021-11-24 |
| US10155929B2 (en) | 2018-12-18 |
| EP2997130A4 (en) | 2017-03-01 |
| JP2021166553A (ja) | 2021-10-21 |
| SG11201509368PA (en) | 2015-12-30 |
| MX2015015840A (es) | 2016-08-11 |
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