CN105506086A - SNP molecular markers related to chicken-fertilization duration time characters and application thereof - Google Patents
SNP molecular markers related to chicken-fertilization duration time characters and application thereof Download PDFInfo
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Abstract
The invention provides SNP molecular markers related to chicken-fertilization duration time characters and an application thereof. TGFB3 genes are subjected to polymorphic researching and association analysis with the association analysis method, and the SNP molecular markers related to the chicken-fertilization duration time characters are found from first introns of the chicken TGFB3 genes for the first time; the SNP molecular markers particularly relate to four SNP molecular markers related to hen-fertilization duration time characters and the positioning and the application of a haplotype formed by the four SNP molecular markers. The SNP molecular markers are obtained through association analysis, and the nucleotide sequences of the SNP molecular markers are shown as SEQ ID NO:1-4. The molecular markers and the haplotype can be used for molecular breeding of chicken-fertilization duration time.
Description
Technical field
The invention belongs to technical field of livestock molecular marker preparation, be specifically related to 4 and be fertilized the haplotype and application thereof that the relevant SNP marker of time length proterties and these 4 SNP form to chicken.
Background technology
The difference of hen fertilization time length proterties (continuing the number of days producing fertile egg after an artificial insemination) directly affects the utilization ratio of test-tube frequency and stock cock.Thus affect economic benefit, be the important reproductive trait during a poultry is produced.In kind of chicken produces, artificial insemination frequency be 5 days once, every 50 hens need outfit 1 stock cock.If carry out seed selection to this proterties, test-tube frequency can be reduced, make test-tube labor force pay minimizing.What hen can also be made to be subject to because of artificial insemination stress reduce greatly, improves hatchable egg rate, reduces the rate of fertilization decline because artificial insemination causes.The utilization ratio of stock cock can also be improved simultaneously, reduce the number of animals raised of stock cock, cost-saving.But this proterties tolerance is got up cumbersome, need the raising of single cage, individual volume tracing and economic input large; Seldom have from the report of hereditary angle to this proterties.
The means of molecular breeding are utilized to be reduce one of artificial insemination frequency and the effective way improving stock cock utilization ratio to improve the reproductive trait of hen.It is combining site (UVJ) in hen uterus and vagina that hen can continue to produce the Basic of Biology of fertile egg, the special tubulose structure organization-Sperm storing duct (SST) of one that existence is caved in by UVJ tissue epithelial and formed, SST has the function of storing and maintaining spermatozoon activity.The storage of sperm in SST directly has influence on hen and continues fertilization time.Research finds, in order to ensure that sperm can be survived in SST, hen has been cooked a series of adjustment on gene expression dose.And to affect the important factor in order that sperm stores in SST be that SST regulates and controls the immune response of sperm.High expression in transforming growth factor and acceptor gene thereof vagina joint portion, the uterus tissue after artificial insemination, especially the expression level of TGFB3 gene before and after semen deposition presents noticeable change, TGFBs gene family has biological function widely, as regulating cell propagation, differentiation and migration etc., have in its secretion in regulation and control female reproductive tract cytokine of report and immune response simultaneously and there is local immunosuppression effect.Infer that the expression of TGFB3 gene can suppress this place T-cell and B-cell for the immunne response (sperm can be regarded as a kind of allogenic material to hen uterus) of sperm, thus ensure that sperm can be stored in (Das in SST, 2006), TGFB3 gene can be used as the relevant candidate gene of time length proterties of being fertilized to hen.
Below work and to subsidize by project of national nature science fund project, project name " chicken stores screening and the Molecular Mechanism thereof of smart capacity variance relative new gene ", project approval number: 31372301.
Summary of the invention
The object of the invention is to the defect overcoming prior art, provide 4 to be fertilized the haplotype and application thereof that the relevant SNP marker of time length proterties and these 4 SNP form to chicken.The present invention uses the method for whole-genome association to find to be fertilized with chicken the relevant SNP marker of time length proterties and haplotype, to be fertilized the SNP marker and the application of haplotype in marker assisted selection that time length proterties is correlated with in this, as chicken.
The present invention is achieved through the following technical solutions:
The SNP marker that chicken fertilization time length proterties is relevant, is characterized in that: the SNP marker that described chicken fertilization time length proterties is relevant is the SNP marker that the chicken fertilization time length proterties in the First Intron of chicken TGFB3 (transforminggrowthfactor-β 3) gene is relevant.
As preferably, the nucleotide sequence of the SNP marker that chicken fertilization time length proterties provided by the present invention is correlated with is SEQIDNO:1, SEQIDNO:2, SEQIDNO:3 or SEQIDNO:4;
When the SNP marker that described chicken fertilization time length proterties is relevant is SEQIDNO:1, the nucleotide sequence of described SEQIDNO:1 is:
TCCTCTGCCTCAGGCCAGAACAGCTTGGCTCCCTAGTGCCGGCCCCAGCRTTCGGTGCAAGGCCACAGGTGAGAGGGAGGAGATCCCATACTTTGCTCCA；
In the sequence of described SEQIDNO:1, the base R of the 50th is C or T, causes polymorphism;
When the SNP marker that described chicken fertilization time length proterties is relevant is SEQIDNO:2, the nucleotide sequence of described SEQIDNO:2 is:
GGATCCCTCCACCAGCAGAGAAGCCCTGACCCTTCTGCTTAAGTCCCTTRGCAGAGTTGTTCCTCTGACTGTCGCAGTACAGTACAGTTGGTGTTAATCC；
In the sequence of described SEQIDNO:2, the base R of the 50th is A or G, causes polymorphism;
When the SNP marker that described chicken fertilization time length proterties is relevant is SEQIDNO:3, the nucleotide sequence of described SEQIDNO:3 is:
TTATTGGAAAAGAAATGTTTGCTTTCAGTGTTCCTTTCATCAGCAATTCRGTGGGGACAGTTCCTGGCACCTTTAGCACAGGTGCTCATCTGGAGTTTGG；
In the sequence of described SEQIDNO:3, the base R of the 50th is T or C, causes polymorphism;
When the SNP marker that described chicken fertilization time length proterties is relevant is SEQIDNO:4, the nucleotide sequence of described SEQIDNO:4 is:
ACCTTTAGCACAGGTGCTCATCTGGAGTTTGGTTGGAACTCTTGGCGTCRGCGGGCAAGATAAGGTCTGGTTCAGGTTTGGAAGCGGTTTCTGGCCATGC；
In the sequence of described SEQIDNO:4, the base R of the 50th is T or C, causes polymorphism.
The SNP marker that above-mentioned chicken fertilization time length proterties is relevant forms a haplotype.
The SNP marker that above-mentioned chicken fertilization time length proterties is correlated with is used alone or in combination the application in chicken fertilization time length proterties detects.
Based on the preparation method of the SNP marker that foregoing chicken fertilization time length proterties is correlated with, it is characterized in that: said method comprising the steps of:
1) laboratory sample collection:
Described laboratory sample is test chicken blood sample;
2) mensuration of chicken fertilization time length proterties:
After measuring an artificial insemination, test chicken continues the number of days producing fertile egg, and replicate measurement three times, gets the mean value of three measured results, as the time that the fertilization of this test chicken continues;
3) extraction of chicken genomic dna and detection:
Adopt phenol chloroform method to extract chicken genomic dna from test chicken blood sample, then judge the integrity of DNA with gel electrophoresis; Quality examination is carried out to the DNA after extracting;
4) genotype judges and genotype and chicken are fertilized the association analysis of time length:
By the genomic dna extracted in test chicken blood sample, adopt the method for PCR and restriction fragment length polymorphism, gene type assay is carried out to test chicken, utilize the statistical study of being described property of SPSS19 statistical analysis software, time length proterties of being fertilized by test chicken and TGFB3 genotype carry out association analysis.
Advantage of the present invention is:
The present invention's used the method for association analysis to the carrying out of TGFB3 gene polymorphic research and association analysis, and finding chicken to be fertilized the relevant SNP marker of time length proterties at the First Intron of chicken TGFB3 gene first, this SNP marker is specifically related to 4 and locates to the be fertilized relevant SNP marker of time length proterties and the haplotype that is made up of these 4 SNP of hen and apply.This SNP marker is obtained by association analysis, and its nucleotide sequence is as shown in SEQIDNO:1-4.Molecule marker provided by the present invention and haplotype can be used in the molecular breeding of chicken fertilization time length.
Accompanying drawing explanation
Fig. 1 is techniqueflow chart of the present invention;
Fig. 2 utilizes Haploview software to carry out linkage disequilibrium value result to SNP.
Embodiment
The invention provides the SNP marker that a kind of chicken fertilization time length proterties is relevant, the SNP marker that this chicken fertilization time length proterties is relevant is the SNP marker that the chicken fertilization time length proterties in the First Intron of chicken TGFB3 (transforminggrowthfactor-β 3) gene is relevant.
The nucleotide sequence of the SNP marker that chicken fertilization time length proterties provided by the present invention is correlated with is SEQIDNO:1, SEQIDNO:2, SEQIDNO:3 or SEQIDNO:4;
When the SNP marker that chicken fertilization time length proterties is relevant is SEQIDNO:1, the nucleotide sequence of SEQIDNO:1 is:
TCCTCTGCCTCAGGCCAGAACAGCTTGGCTCCCTAGTGCCGGCCCCAGCRTTCGGTGCAAGGCCACAGGTGAGAGGGAGGAGATCCCATACTTTGCTCCA；
In the sequence of SEQIDNO:1, the base R of the 50th is C or T, causes polymorphism;
When the SNP marker that chicken fertilization time length proterties is relevant is SEQIDNO:2, the nucleotide sequence of SEQIDNO:2 is:
GGATCCCTCCACCAGCAGAGAAGCCCTGACCCTTCTGCTTAAGTCCCTTRGCAGAGTTGTTCCTCTGACTGTCGCAGTACAGTACAGTTGGTGTTAATCC；
In the sequence of SEQIDNO:2, the base R of the 50th is A or G, causes polymorphism;
When the SNP marker that chicken fertilization time length proterties is relevant is SEQIDNO:3, the nucleotide sequence of SEQIDNO:3 is:
TTATTGGAAAAGAAATGTTTGCTTTCAGTGTTCCTTTCATCAGCAATTCRGTGGGGACAGTTCCTGGCACCTTTAGCACAGGTGCTCATCTGGAGTTTGG；
In the sequence of SEQIDNO:3, the base R of the 50th is T or C, causes polymorphism;
When the SNP marker that chicken fertilization time length proterties is relevant is SEQIDNO:4, the nucleotide sequence of SEQIDNO:4 is:
ACCTTTAGCACAGGTGCTCATCTGGAGTTTGGTTGGAACTCTTGGCGTCRGCGGGCAAGATAAGGTCTGGTTCAGGTTTGGAAGCGGTTTCTGGCCATGC；
In the sequence of SEQIDNO:4, the base R of the 50th is T or C, causes polymorphism.
The SNP marker that chicken fertilization time length proterties is relevant forms a haplotype.
The SNP marker that chicken fertilization time length proterties is correlated with is used alone or in combination the application in chicken fertilization time length proterties detects.
Based on the preparation method of the SNP marker that foregoing chicken fertilization time length proterties is correlated with, the method comprises the following steps:
1) laboratory sample collection:
Described laboratory sample comprises test chicken blood sample;
2) mensuration of chicken fertilization time length proterties:
After measuring an artificial insemination, test chicken continues the number of days producing fertile egg, and replicate measurement three times, gets the mean value of three measured results, as the time that the fertilization of this test chicken continues;
3) extraction of chicken genomic dna and detection:
Adopt phenol chloroform method to extract chicken genomic dna from test chicken blood sample, then judge the integrity of DNA with gel electrophoresis; Quality examination is carried out to the DNA after extracting;
4) genotype judges and genotype and chicken are fertilized the association analysis of time length:
By the genomic dna extracted in test chicken blood sample, adopt the method for PCR and restriction fragment length polymorphism, gene type assay is carried out to test chicken, utilize the statistical study of being described property of SPSS19 statistical analysis software, time length proterties of being fertilized by test chicken and TGFB3 genotype carry out association analysis.
Below in conjunction with accompanying drawing Fig. 1 and Fig. 2, technical scheme provided by the present invention is described in detail:
One, laboratory sample
Experimental chicken group plants the red No. 1 father and mother godmother chicken (180d age in days) in 652 capital of chicken house from Jing Zhou, Hubei exit of valley company limited, and single cage is raised, and performs 16:8 (L::D) 14h light regime.Free choice feeding, drinking-water, whole feed mode, rearing conditions etc. remain consistent, are ordinary method.
Two, the mensuration of chicken fertilization time length
Mixed semen semen deposition is carried out to hen, after recording an artificial insemination, collect and lay eggs and record laying eggs the date of every piece of egg, within every 8 days, enter to incubate once, enter to incubate and detect fertilization situation according to egg afterwards in 8 days, add up the fertilization situation in every hen artificial insemination 20 days, after adding up the artificial insemination of every hen, continue the number of days producing fertile egg.Repeat statistics three times, using the mean value of three statistics as the fertilization time length of this hen.Simultaneously to every hen oxter venous blood collection.
Three, the extraction of chicken genomic dna and detection
Test adopts phenol chloroform method to extract chicken genomic dna from chicken blood tissue, and concrete operations are as follows:
1) remove 60 μ l chicken blood samples, add 500 μ lSET, 25 μ l10%SDS and 5 μ l Proteinase Ks and spend the night in 55 DEG C of water-baths digestion;
2) in above-mentioned Digestive system, add isopyknic phenol/chloroform/primary isoamyl alcohol (25:24:1), slowly put upside down centrifuge tube 6min, the centrifugal 6min of 10000r/min.
3) draw supernatant, add isopyknic chloroform/primary isoamyl alcohol (24:1), slowly put upside down centrifuge tube 6min, the centrifugal 6min of 10000r/min.
4) draw supernatant, add the dehydrated alcohol of 2 times of volumes, fully, now may there is flocks, the centrifugal 6min of 10000r/min in vibration mixing 15 seconds.Abandon supernatant, residual ethanol of at room temperature volatilizing, add appropriate pure water dissolving DNA.
5) get 2 μ L previous step gained solution D NA solution and the mixing of 1 μ L sample loading buffer, be splined on the sepharose of 1.2%, 120V electrophoresis about 20 minutes, observes electrophoresis result under ultraviolet lamp and takes pictures, to judge the integrity of DNA.Carry out quality examination with NanoDrop2000 nucleic acid-protein analyser (ThermoFisherScientific, USA) to the DNA after extraction, the ratio of A260/A280 is between 1.7-2.1, and it is qualified that A260/A230 is judged as between 1.8-2.2.Carry out concentration determination to qualified DNA, be then diluted to 200ng/ μ L by unified for concentration, the refrigerator putting into-20 DEG C is deposited.Underproof DNA sample then needs again to extract.
Four, SNPs detection, genotype judge and haplotype analysis
According to TGFB3 gene order design primer, the hen that 15 the fertilization time length of increasing respectively are long and 15 fertilization time length short hen DNA biased sample, amplified production serves Hai Shenggong order-checking after reclaiming.By the difference between comparison fertilization time length long and short hen TGFB3 gene order, filter out 4 SNPs sites.With the genome DNA sample extracted in 652 chicken bloods for template, amplification comprises the genome sequence in these 4 SNPs sites respectively.Carry out enzyme with restriction enzyme BsaJI to amplified production to cut.4 genotype are judged according to restriction enzyme length polymorphism.And utilize Haploview and PHASE software to carry out haplotype analysis to these 4 SNPs.
Five, association analysis
Utilize SPSS software to 652 hens fertilization time length being described property of proterties statistics, and be fertilized by hen time length proterties and TGFB3 genotype carry out association analysis.
Six, interpretation of result
Show that AA (TT) genotype has 171 in 652 individualities to chicken TGFB3 gene polymorphism sites T230C genotype call results, AB (TC) genotype has 448, and BB (CC) genotype has 33.The proterties analyzed is the chicken fertilization time length.Result shows: the SNP site T230C of TGFB3 gene and chicken time length of being fertilized is that significant correlation (P<0.05), SNP site T230C and chicken are fertilized time length association analysis and have the genotype of remarkably influenced in table 1 to chicken time length of being fertilized.
Table 1SNPs site T230C (TGFB3) genotype and fertilization time length
Note: * represents significant difference, P<0.05; * represents difference extremely significantly P<0.01; In table, character value is mean number ± standard error.
As shown in Table 1, the AB genotypic fertilization time length is significantly longer than AA genotype (p<0.01).Therefore AB (TC) genotype is the favourable mark of hen fertilization time length.
Show that AB (AG) genotype has 300 in 652 individualities to chicken TGFB3 gene polymorphism sites A477G genotype call results, BB (GG) genotype has 352.The proterties analyzed is the chicken fertilization time length.Result shows: the SNP site A477G of TGFB3 gene and chicken time length of being fertilized is that significant correlation (P<0.05), SNP site A477G and chicken are fertilized time length association analysis and have the genotype of remarkably influenced in table 2 to chicken time length of being fertilized.
Table 2SNPs site A477G (TGFB3) genotype and fertilization time length
Note: * represents significant difference, P<0.05; * represents difference extremely significantly P<0.01; In table, character value is mean number ± standard error.
As shown in Table 2, the AB genotypic fertilization time length is significantly longer than BB genotype (p<0.05).Therefore AB (AG) genotype is the favourable mark of hen fertilization time length.
Show that AA (TT) genotype has 290 in 652 individualities to chicken TGFB3 gene polymorphism sites T607C genotype call results, AB (TC) genotype has 300, and BB (CC) genotype has 62.The proterties analyzed is the chicken fertilization time length.Result shows: the SNP site T607C of TGFB3 gene and chicken time length of being fertilized is that significant correlation (P<0.05), SNP site T607C and chicken are fertilized time length association analysis and have the genotype of remarkably influenced in table 3 to chicken time length of being fertilized.
Table 3SNPs site T607C (TGFB3) genotype and fertilization time length
Note: * represents significant difference, P<0.05; * represents difference extremely significantly P<0.01; In table, character value is mean number ± standard error.
As shown in Table 3, AB (TC) the genotypic fertilization time length is significantly longer than AA (TT) genotype and BB (CC) genotype (p<0.05).Therefore AB (TC) genotype is the favourable mark of hen fertilization time length.
Show that AA (TT) genotype has 227 in 652 individualities to chicken TGFB3 gene polymorphism sites T675C genotype call results, AB (TC) genotype has 316, and BB (CC) genotype has 109.The proterties analyzed is the chicken fertilization time length.Result shows: the SNP site T675C of TGFB3 gene and chicken time length of being fertilized is significant correlation (P < 0.01), and SNP site T675C and chicken are fertilized time length association analysis and have the genotype of remarkably influenced in table 4 to chicken time length of being fertilized.
Table 4SNPs site T675C (TGFB3) genotype and fertilization time length
Note: * represents significant difference, P<0.05; * represents difference extremely significantly P<0.01; In table, character value is mean number ± standard error.
As shown in Table 4, AB (TC) the genotypic fertilization time length is significantly longer than AA (TT) genotype and BB (CC) genotype (p<0.01).Therefore AB (TC) genotype is the favourable mark of hen fertilization time length.
From haplotype analysis, the linkage disequilibrium coefficient of these 4 SNP (T230C, A477G, T607C and T675C) (D ') be 1, namely there is complete linkage imbalance between these 4 sites, simultaneously these 4 SNP can component frequency 6 kinds of haplotypes being greater than 1%.To be fertilized the association analysis result of time length according to hen, haplotype combination TGTC/CACT is the favourable mark of hen fertilization time length.The haplotype of these 4 SNP marker and formation thereof can apply in the molecular breeding of chicken, and then improves the reproductive performance of chicken, the results are shown in Table 5 and table 6.
Table 5TGFB3 gene haplotype type and hen are fertilized the association analysis of time length
As shown in Table 5: the haplotype of TGFB3 gene and hen time length of being fertilized is pole significant correlation (p<0.01).
Table 6 haplotype type and fertilization time length
A-B: difference extremely significantly (P < 0.01).In table, character value is mean number ± standard error.
To be fertilized the association analysis result of time length according to hen, the haplotype TGTC/CACT that TFBF3 gene 4 SNPs are formed is the favourable mark of hen fertilization time length.The haplotype of these 4 SNP marker and formation thereof can apply in the molecular breeding of hen fertilization time length proterties, and then puies forward the productivity effect of laying breed.
Claims (5)
1. the SNP marker that chicken fertilization time length proterties is relevant, is characterized in that: the SNP marker that described chicken fertilization time length proterties is correlated with is the SNP marker that the chicken fertilization time length proterties in the First Intron of chicken TGFB3 gene is relevant.
2. the SNP marker that chicken fertilization time length proterties according to claim 1 is relevant, is characterized in that: the nucleotide sequence of the SNP marker that described chicken fertilization time length proterties is correlated with is SEQIDNO:1, SEQIDNO:2, SEQIDNO:3 or SEQIDNO:4;
When the SNP marker that described chicken fertilization time length proterties is relevant is SEQIDNO:1, the nucleotide sequence of described SEQIDNO:1 is:
TCCTCTGCCTCAGGCCAGAACAGCTTGGCTCCCTAGTGCCGGCCCCAGC
RTTCGGTGCAAGGCCACAGGTGAGAGGGAGGAGATCCCATACTTTGCTCCA；
In the sequence of described SEQIDNO:1, the base R of the 50th is C or T, causes polymorphism;
When the SNP marker that described chicken fertilization time length proterties is relevant is SEQIDNO:2, the nucleotide sequence of described SEQIDNO:2 is:
GGATCCCTCCACCAGCAGAGAAGCCCTGACCCTTCTGCTTAAGTCCCTT
RGCAGAGTTGTTCCTCTGACTGTCGCAGTACAGTACAGTTGGTGTTAATCC；
In the sequence of described SEQIDNO:2, the base R of the 50th is A or G, causes polymorphism;
When the SNP marker that described chicken fertilization time length proterties is relevant is SEQIDNO:3, the nucleotide sequence of described SEQIDNO:3 is:
TTATTGGAAAAGAAATGTTTGCTTTCAGTGTTCCTTTCATCAGCAATTC
RGTGGGGACAGTTCCTGGCACCTTTAGCACAGGTGCTCATCTGGAGTTTGG；
In the sequence of described SEQIDNO:3, the base R of the 50th is T or C, causes polymorphism;
When the SNP marker that described chicken fertilization time length proterties is relevant is SEQIDNO:4, the nucleotide sequence of described SEQIDNO:4 is:
ACCTTTAGCACAGGTGCTCATCTGGAGTTTGGTTGGAACTCTTGGCGTC
RGCGGGCAAGATAAGGTCTGGTTCAGGTTTGGAAGCGGTTTCTGGCCATGC；
In the sequence of described SEQIDNO:4, the base R of the 50th is T or C, causes polymorphism.
3. the SNP marker that chicken fertilization time length proterties according to claim 2 is relevant forms a haplotype.
4. the SNP marker that chicken fertilization time length proterties according to claim 3 is correlated with is used alone or in combination the application in chicken fertilization time length proterties detects.
5. a preparation method for the SNP marker of being correlated with based on chicken as claimed in claim 2 fertilization time length proterties, is characterized in that: said method comprising the steps of:
1) laboratory sample collection:
Described laboratory sample is test chicken blood sample;
2) mensuration of chicken fertilization time length proterties:
After measuring an artificial insemination, test chicken continues the number of days producing fertile egg, and replicate measurement three times, gets the mean value of three measured results, as the time that the fertilization of this test chicken continues;
3) extraction of chicken genomic dna and detection:
From test chicken blood sample, extract chicken genomic dna, then judge the integrity of DNA with gel electrophoresis; Quality examination is carried out to the DNA after extracting;
4) genotype judges and genotype and chicken are fertilized the association analysis of time length:
By the genomic dna extracted in test chicken blood sample, adopt the method for PCR and restriction fragment length polymorphism, gene type assay is carried out to test chicken, utilize the statistical study of being described property of SPSS19 statistical analysis software, time length proterties of being fertilized by test chicken and TGFB3 genotype carry out association analysis.
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| CN110499369A (en) * | 2018-05-17 | 2019-11-26 | 华中农业大学 | For selecting hen to store up the SNP marker and its application of smart ability |
| CN110551824A (en) * | 2018-05-31 | 2019-12-10 | 华中农业大学 | SNP molecular marker related to sperm storage capacity of hen and application thereof |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103898231A (en) * | 2014-04-17 | 2014-07-02 | 华中农业大学 | SNP (Single Nucleotide Polymorphism) molecular marker related to pork pH characteristics and application thereof |
| CN104694538A (en) * | 2015-01-28 | 2015-06-10 | 中国农业大学 | SNP molecular marker related to chicken polydactyly character and application thereof |
| CN104862388A (en) * | 2015-05-05 | 2015-08-26 | 华中农业大学 | Single nucleotide polymorphism (SNP) molecular markers related to pig effective teat pair number traits and application |
-
2015
- 2015-12-25 CN CN201511003811.8A patent/CN105506086B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103898231A (en) * | 2014-04-17 | 2014-07-02 | 华中农业大学 | SNP (Single Nucleotide Polymorphism) molecular marker related to pork pH characteristics and application thereof |
| CN104694538A (en) * | 2015-01-28 | 2015-06-10 | 中国农业大学 | SNP molecular marker related to chicken polydactyly character and application thereof |
| CN104862388A (en) * | 2015-05-05 | 2015-08-26 | 华中农业大学 | Single nucleotide polymorphism (SNP) molecular markers related to pig effective teat pair number traits and application |
Non-Patent Citations (2)
| Title |
|---|
| 许东伟: "京红京粉祖代蛋鸡贮精能力重复率估计、差异相关基因筛选及cSNP关联分析", 《万方数据知识服务平台》 * |
| 陈鹏: "罗斯308父母代母鸡贮精能力重复率的估计及该性状与cSNP的关联分析", 《万方数据知识服务平台》 * |
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