CN105486625A - Cell counting device and method based on Terahertz time-domain spectroscopy technology - Google Patents
Cell counting device and method based on Terahertz time-domain spectroscopy technology Download PDFInfo
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- CN105486625A CN105486625A CN201610061076.4A CN201610061076A CN105486625A CN 105486625 A CN105486625 A CN 105486625A CN 201610061076 A CN201610061076 A CN 201610061076A CN 105486625 A CN105486625 A CN 105486625A
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- 238000000034 method Methods 0.000 title claims abstract description 15
- 238000005516 engineering process Methods 0.000 title claims abstract description 13
- 238000001328 terahertz time-domain spectroscopy Methods 0.000 title abstract 2
- 238000001514 detection method Methods 0.000 claims abstract description 18
- 238000001228 spectrum Methods 0.000 claims abstract description 13
- 229910052751 metal Inorganic materials 0.000 claims abstract description 12
- 239000002184 metal Substances 0.000 claims abstract description 12
- 239000000463 material Substances 0.000 claims abstract description 8
- 229920001577 copolymer Polymers 0.000 claims abstract description 6
- 238000004458 analytical method Methods 0.000 claims abstract description 4
- 239000000758 substrate Substances 0.000 claims description 26
- 239000006285 cell suspension Substances 0.000 claims description 20
- 238000010521 absorption reaction Methods 0.000 claims description 14
- 125000004122 cyclic group Chemical group 0.000 claims description 5
- 230000003595 spectral effect Effects 0.000 claims description 5
- 239000007853 buffer solution Substances 0.000 claims description 2
- 238000004611 spectroscopical analysis Methods 0.000 claims description 2
- 239000000725 suspension Substances 0.000 claims description 2
- 238000002594 fluoroscopy Methods 0.000 abstract 3
- 229920000089 Cyclic olefin copolymer Polymers 0.000 description 3
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 230000005540 biological transmission Effects 0.000 description 2
- 229910052802 copper Inorganic materials 0.000 description 2
- 239000010949 copper Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 1
- 239000004411 aluminium Substances 0.000 description 1
- 229910052782 aluminium Inorganic materials 0.000 description 1
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- HGAZMNJKRQFZKS-UHFFFAOYSA-N chloroethene;ethenyl acetate Chemical compound ClC=C.CC(=O)OC=C HGAZMNJKRQFZKS-UHFFFAOYSA-N 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 230000001678 irradiating effect Effects 0.000 description 1
- 239000011777 magnesium Substances 0.000 description 1
- 229910052749 magnesium Inorganic materials 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 239000007769 metal material Substances 0.000 description 1
- 238000010899 nucleation Methods 0.000 description 1
- 230000000704 physical effect Effects 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 239000010453 quartz Substances 0.000 description 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N silicon dioxide Inorganic materials O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
- G01N21/35—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using infrared light
- G01N21/3581—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using infrared light using far infrared light; using Terahertz radiation
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N2015/1006—Investigating individual particles for cytology
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N2015/1022—Measurement of deformation of individual particles by non-optical means
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N2015/1024—Counting particles by non-optical means
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- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Biochemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Dispersion Chemistry (AREA)
- Toxicology (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
- Optical Measuring Cells (AREA)
Abstract
The invention relates to a cell counting device and method based on the Terahertz time-domain spectroscopy technology. The device comprises a support, a Terahertz light source, a Terahertz detector, a metal baffle and a sample fluoroscopy plate, wherein the Terahertz light source and the Terahertz detector are correspondingly arranged on the support, the metal baffle and the sample fluoroscopy plate are distributed vertically and arranged between the Terahertz light source and the Terahertz detector, and a light source hole is correspondingly formed in the metal baffle. The sample fluoroscopy plate is made of a cycloolefin copolymer material, detection accuracy is improved, and the overall device is simple in structure and convenient to operate. According to the cell counting method using the device, cells are counted through the steps of sample dripping, spectrum detection and analysis, the accuracy is high, the detection period is short, and operation is simple.
Description
Technical field
Biological substance technical field is tested or analyzed to the chemistry or physical property that the invention belongs to by means of measuring biological substance, relates to one and carry out Cytometric device and method based on terahertz time-domain spectroscopic technology.
Background technology
In cell cultivation process, cell count is absolutely necessary a step.Traditional method for cell count adopts tally to pass through artificial counting under the microscope, because the method is to lower concentration by cell dilution, drawn by formulae discovery, certain error can be caused, also can be with due to human factor simultaneously and serve subjective error.
Because tera-hertz spectra is to the strong absorption of water, so by gathering the terahertz light spectrum information of cell suspension, can first pass through the terahertz light spectral absorption coefficient of the cell suspension calculating variable concentrations gradient, the tera-hertz spectra absorption curve of Criterion; Then gather the tera-hertz spectra of tested cell suspension, just can carry out cell sample counting by typical curve.The method is simple to operation, consuming time short, there is not subjective factor, therefore error is little, calculates accurately.
Summary of the invention
In view of this, the object of the present invention is to provide one to carry out Cytometric device and method based on terahertz time-domain spectroscopic technology, by the detection feature of tera-hertz spectra, carry out the cell count of various types of cells system, for cell seeding and going down to posterity etc. provides reference.
For achieving the above object, the invention provides following technical scheme:
One carries out Cytometric device based on terahertz time-domain spectroscopic technology, comprise support, correspondence is arranged on Terahertz light source on support and terahertz detector, to distribute and the metal baffle be arranged between Terahertz light source and terahertz detector and sample perspective board up and down, in described metal baffle, correspondence is provided with light source hole;
Described sample perspective board comprises substrate and the cover plate covered on substrate, and described substrate is provided with for placing the groove detecting sample; Described support is also provided with the shackle for placing sample perspective board.
Further, described substrate and cover plate are the circular transparent sheet adopting cyclic olefine copolymer material to make.
Further, the diameter of described substrate is 50-100mm, and thickness is 0.1-2mm, and the diameter of described cover plate is 50-100mm, and thickness is 0.05-1mm.
Further, for dripping cell suspension in the groove of described substrate, described cell suspension refers to and is evenly dispersed in PBS buffer solution by cell, and the volume of suspension is 7 μ L-1155 μ L; The diameter of groove is 30-70mm, and thickness is 0.01-0.3mm.
Further, transparent outer cover is also provided with in device outside.
Present invention also offers one and carry out Cytometric method based on terahertz time-domain spectroscopic technology: comprise the following steps:
A, a sample: cell suspension is instilled sample groove;
B, detection: utilize terahertz light spectrum detection device to gather the time-domain spectroscopy information of cell suspension, asked for the absorption coefficient of this sample by the thickness information of sample cell;
C, analysis: the cell count curve this absorption coefficient being substituted into this clone, calculates the cell number of this sample.
Further, the cell count curve in described step c is the standard absorption coefficient curve of the clone of sample being carried out to the acquisition of concentration gradient spectral detection.
Beneficial effect of the present invention is: the substrate and the cover plate that 1) form sample perspective board adopt cyclic olefine copolymer material to make, there is the absorption of low Terahertz, low-refraction, high transmission rate feature, be better than the existing sample arrangement for perspective detected for tera-hertz spectra prepared by materials such as quartz, tygon, improve accuracy of detection; 2) utilize the strong absorption characteristic of the water of tera-hertz spectra uniqueness to carry out cell count, simple to operate, accuracy is high, shortens sense cycle, is better than existing detection method.
Accompanying drawing explanation
In order to make object of the present invention, technical scheme and beneficial effect clearly, the invention provides following accompanying drawing and being described:
Fig. 1 is structural representation of the present invention;
Fig. 2 is the stereographic map of terahertz light spectrum detection device.
Embodiment
Below in conjunction with accompanying drawing, the preferred embodiments of the present invention are described in detail.
As shown in Figure 1, the tera-hertz spectra that utilizes described in the present embodiment carries out Cytometric device, comprise support, correspondence is arranged on Terahertz light source on support and terahertz detector, to distribute and the metal baffle be arranged between Terahertz light source and terahertz detector and sample perspective board up and down, in described metal baffle, correspondence is provided with light source hole.
In the present embodiment, Terahertz light source, metal baffle, sample perspective board and terahertz detector set gradually from top to bottom, sample perspective board is wherein placed on a shackle, and shackle is the same with Terahertz light source, metal baffle and terahertz detector to be all fixed on support; Metal baffle wherein mainly for stopping Terahertz light source, preventing thz beam scattering towards periphery, therefore can be arbitrary metal materials such as copper, iron, aluminium, magnesium, adopting copper material in the present embodiment.
Sample perspective board in the present embodiment comprises substrate and the cover plate covered on substrate, and described substrate is provided with for placing the groove detecting sample; During operation, measuring samples is placed in groove, covers cover plate, convenient to operation.
In the present embodiment, described substrate and cover plate are the circular transparent sheet adopting cyclic olefine copolymer material to make, and the diameter of substrate is 55mm, and thickness is 0.1mm, and the diameter of cover plate is 55mm, and thickness is 0.05mm; Cyclic olefine copolymer (be called for short COPs (CycloOlefinPolymers) or COCs (CycloOlefinCopolymers)) has that low Terahertz absorbs, the advantageous property of low-refraction and high transmission rate, be suitable as the arrangement for perspective of sample bearing device or detection sample, carrying material can be reduced in testing process to the influence factor of testing result.
If the diameter of substrate is 100mm, when thickness is 2mm, corresponding cover plate can select diameter to be 100mm, and thickness is the such specification of 1mm; Certainly, when meeting hardness requirement, the thickness of substrate and cover plate should arrange thinner as far as possible, when the diameter of substrate is 90mm, thickness is 1.3mm, the diameter of corresponding cover plate is 90mm, thickness is for reaching the hardness requirement needed for detection during 0.6mm time, should select substrate and the cover plate of this kind of slim specification as far as possible.
In the present embodiment, for dripping cell suspension in the groove of substrate, the volume of described cell suspension is between 7 μ L-1155 μ L, and groove diameter size is between 30-70mm, and thickness is between 0.01-0.3mm, the volume of concrete cell suspension is selected according to the actual conditions detected, wherein, the volume of cell suspension drips should be corresponding with the groove size on substrate, when the amount of cell suspension is more, the diameter of substrate also should be comparatively large, and thickness also should be thicker.
In the present embodiment, pick-up unit outside is also provided with transparent glass outer, and assurance device inside realizes constant humidity, and avoid because air flowing has an impact to measurement, certainly, transparent plastic housing can reach this object equally.
Present invention also offers one utilizes terahertz light spectrum detection device to carry out Cytometric method, comprises the following steps:
A, a sample: cell suspension is dropped in the groove of substrate, cover cover plate;
B, detection: open Terahertz light source irradiating cell suspension, the terahertz detector being simultaneously arranged on below gathers the spectral information of cell suspension, by the absorption coefficient of this cell suspension of THICKNESS CALCULATION of groove;
C, analysis: the cell count curve this absorption coefficient being substituted into this clone, calculate the cell number of this sample, standard cell counts curve is wherein the standard absorption coefficient curve of the clone of sample being carried out to the acquisition of concentration gradient spectral detection, and process is simple, and accuracy is high.
What finally illustrate is, above preferred embodiment is only in order to illustrate technical scheme of the present invention and unrestricted, although by above preferred embodiment to invention has been detailed description, but those skilled in the art are to be understood that, various change can be made to it in the form and details, and not depart from claims of the present invention limited range.
Claims (7)
1. one kind is carried out Cytometric device based on terahertz time-domain spectroscopic technology, it is characterized in that: comprise support, correspondence is arranged on Terahertz light source on support and terahertz detector, to distribute and the metal baffle be arranged between Terahertz light source and terahertz detector and sample perspective board up and down, in described metal baffle, correspondence is provided with light source hole;
Described sample perspective board comprises substrate and the cover plate covered on substrate, and described substrate is provided with for placing the groove detecting sample; Described support is also provided with the shackle for placing sample perspective board.
2. one according to claim 1 carries out Cytometric device based on terahertz time-domain spectroscopic technology, it is characterized in that: described substrate and cover plate are the circular transparent sheet adopting cyclic olefine copolymer material to make.
3. one according to claim 1 carries out Cytometric device based on terahertz time-domain spectroscopic technology, it is characterized in that: the diameter of described substrate is 50-100mm, and thickness is 0.1-2mm, and the diameter of described cover plate is 50-100mm, and thickness is 0.05-1mm.
4. one according to claim 1 carries out Cytometric device based on terahertz time-domain spectroscopic technology, it is characterized in that: for dripping cell suspension in the groove of described substrate, described cell suspension refers to and is evenly dispersed in PBS buffer solution by cell, and the volume of suspension is 7 μ L-1155 μ L; The diameter of groove is 30-70mm, and thickness is 0.01-0.3mm.
5. one according to claim 1 carries out Cytometric device based on terahertz time-domain spectroscopic technology, it is characterized in that: be also provided with transparent outer cover in device outside.
6. adopt device according to any one of claim 1 to 5 to carry out a Cytometric method, it is characterized in that: comprise the following steps:
A, a sample: cell suspension is instilled sample groove;
B, detection: utilize terahertz light spectrum detection device to gather the time-domain spectroscopy information of cell suspension, asked for the absorption coefficient of this sample by the thickness information of sample cell;
C, analysis: the cell count curve this absorption coefficient being substituted into this clone, calculates the cell number of this sample.
7. method according to claim 6, is characterized in that: the cell count curve in described step c is the standard absorption coefficient curve of the clone of sample being carried out to the acquisition of concentration gradient spectral detection.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610061076.4A CN105486625B (en) | 2016-01-28 | 2016-01-28 | The device and method of cell count is carried out based on terahertz time-domain spectroscopic technology |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610061076.4A CN105486625B (en) | 2016-01-28 | 2016-01-28 | The device and method of cell count is carried out based on terahertz time-domain spectroscopic technology |
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| Publication Number | Publication Date |
|---|---|
| CN105486625A true CN105486625A (en) | 2016-04-13 |
| CN105486625B CN105486625B (en) | 2018-08-21 |
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| CN201610061076.4A Expired - Fee Related CN105486625B (en) | 2016-01-28 | 2016-01-28 | The device and method of cell count is carried out based on terahertz time-domain spectroscopic technology |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106092952A (en) * | 2016-05-30 | 2016-11-09 | 成都曙光光纤网络有限责任公司 | A kind of central shielding device |
| CN108535219A (en) * | 2018-04-08 | 2018-09-14 | 北京环境特性研究所 | The method for measuring the fitting device of reflectivity and making the sample for measuring reflectivity |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111551514A (en) * | 2020-05-08 | 2020-08-18 | 南京大学 | High-sensitivity terahertz sensor capable of detecting trace cells and detection method |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102305767A (en) * | 2011-05-17 | 2012-01-04 | 中国计量学院 | Microcontroller-based terahertz time-domain spectroscopy automatic sample testing device |
| US20120211659A1 (en) * | 2011-02-17 | 2012-08-23 | Arkray, Inc. | Terahertz Wave Characteristic Measurement Method, Material Detection Method, Measurement Instrument, Terahertz Wave Characteristic Measurement Device and Material Detection Device |
| CN102706804A (en) * | 2012-05-23 | 2012-10-03 | 中国科学院上海应用物理研究所 | Liquid sample cell |
| CN103335976A (en) * | 2013-06-04 | 2013-10-02 | 中国石油大学(北京) | Method for measuring concentrations of sulfate, nitrate solutions by utilizing terahertz time-domain spectroscopy |
| CN103616337A (en) * | 2013-12-18 | 2014-03-05 | 重庆绿色智能技术研究院 | Device and method for detecting bacteria in PM (Particulate Matter) 2.5 by utilizing terahertz spectrum |
| CN203502413U (en) * | 2013-10-28 | 2014-03-26 | 中国人民解放军第三军医大学 | Biological sample detection exposure system based on terahertz |
| CN103792204A (en) * | 2014-01-20 | 2014-05-14 | 郑州市中食农产品加工研究院 | Rapid microbiological detection technology based on terahertz time-domain spectroscopy |
-
2016
- 2016-01-28 CN CN201610061076.4A patent/CN105486625B/en not_active Expired - Fee Related
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20120211659A1 (en) * | 2011-02-17 | 2012-08-23 | Arkray, Inc. | Terahertz Wave Characteristic Measurement Method, Material Detection Method, Measurement Instrument, Terahertz Wave Characteristic Measurement Device and Material Detection Device |
| CN102305767A (en) * | 2011-05-17 | 2012-01-04 | 中国计量学院 | Microcontroller-based terahertz time-domain spectroscopy automatic sample testing device |
| CN102706804A (en) * | 2012-05-23 | 2012-10-03 | 中国科学院上海应用物理研究所 | Liquid sample cell |
| CN103335976A (en) * | 2013-06-04 | 2013-10-02 | 中国石油大学(北京) | Method for measuring concentrations of sulfate, nitrate solutions by utilizing terahertz time-domain spectroscopy |
| CN203502413U (en) * | 2013-10-28 | 2014-03-26 | 中国人民解放军第三军医大学 | Biological sample detection exposure system based on terahertz |
| CN103616337A (en) * | 2013-12-18 | 2014-03-05 | 重庆绿色智能技术研究院 | Device and method for detecting bacteria in PM (Particulate Matter) 2.5 by utilizing terahertz spectrum |
| CN103792204A (en) * | 2014-01-20 | 2014-05-14 | 郑州市中食农产品加工研究院 | Rapid microbiological detection technology based on terahertz time-domain spectroscopy |
Non-Patent Citations (1)
| Title |
|---|
| 全国高等教育自学考试指导委员会组编: "《诊断学(二)（附：诊断学(二)自学考试大纲）》", 30 April 2001, 湖南科学技术出版社 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106092952A (en) * | 2016-05-30 | 2016-11-09 | 成都曙光光纤网络有限责任公司 | A kind of central shielding device |
| CN106092952B (en) * | 2016-05-30 | 2019-03-22 | 成都曙光光纤网络有限责任公司 | A kind of central shielding device |
| CN108535219A (en) * | 2018-04-08 | 2018-09-14 | 北京环境特性研究所 | The method for measuring the fitting device of reflectivity and making the sample for measuring reflectivity |
Also Published As
| Publication number | Publication date |
|---|---|
| CN105486625B (en) | 2018-08-21 |
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Granted publication date: 20180821 Termination date: 20220128 |