CN105486625B - The device and method of cell count is carried out based on terahertz time-domain spectroscopic technology - Google Patents
The device and method of cell count is carried out based on terahertz time-domain spectroscopic technology Download PDFInfo
- Publication number
- CN105486625B CN105486625B CN201610061076.4A CN201610061076A CN105486625B CN 105486625 B CN105486625 B CN 105486625B CN 201610061076 A CN201610061076 A CN 201610061076A CN 105486625 B CN105486625 B CN 105486625B
- Authority
- CN
- China
- Prior art keywords
- cell
- sample
- terahertz
- substrate
- cell count
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 238000000034 method Methods 0.000 title claims abstract description 14
- 238000001514 detection method Methods 0.000 claims abstract description 22
- 229910052751 metal Inorganic materials 0.000 claims abstract description 10
- 239000002184 metal Substances 0.000 claims abstract description 10
- 239000000463 material Substances 0.000 claims abstract description 8
- 230000003595 spectral effect Effects 0.000 claims abstract description 7
- 229920001577 copolymer Polymers 0.000 claims abstract description 6
- 125000004122 cyclic group Chemical group 0.000 claims abstract description 6
- 239000000758 substrate Substances 0.000 claims description 25
- 239000006285 cell suspension Substances 0.000 claims description 23
- 238000010521 absorption reaction Methods 0.000 claims description 13
- 238000001228 spectrum Methods 0.000 claims description 11
- 239000007853 buffer solution Substances 0.000 claims description 2
- 238000004611 spectroscopical analysis Methods 0.000 claims description 2
- 239000000725 suspension Substances 0.000 claims description 2
- 239000007788 liquid Substances 0.000 claims 1
- 238000004458 analytical method Methods 0.000 abstract 1
- 229920000089 Cyclic olefin copolymer Polymers 0.000 description 4
- 239000000243 solution Substances 0.000 description 3
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- 229910052802 copper Inorganic materials 0.000 description 2
- 239000010949 copper Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 1
- 239000004698 Polyethylene Substances 0.000 description 1
- 239000004411 aluminium Substances 0.000 description 1
- 229910052782 aluminium Inorganic materials 0.000 description 1
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 150000001925 cycloalkenes Chemical class 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 1
- 239000010931 gold Substances 0.000 description 1
- 229910052737 gold Inorganic materials 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 230000001678 irradiating effect Effects 0.000 description 1
- 239000011777 magnesium Substances 0.000 description 1
- 229910052749 magnesium Inorganic materials 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 239000007769 metal material Substances 0.000 description 1
- 238000010899 nucleation Methods 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- -1 polyethylene Polymers 0.000 description 1
- 229920000573 polyethylene Polymers 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 239000010453 quartz Substances 0.000 description 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N silicon dioxide Inorganic materials O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
- G01N15/10—Investigating individual particles
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
- G01N21/35—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using infrared light
- G01N21/3581—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using infrared light using far infrared light; using Terahertz radiation
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N2015/1006—Investigating individual particles for cytology
-
- G01N2015/1022—
-
- G01N2015/1024—
Abstract
The present invention relates to a kind of device and methods carrying out cell count based on terahertz time-domain spectroscopic technology, the device includes holder, is correspondingly arranged on holder Terahertz light source and terahertz detector are distributed and are arranged the metal baffle between Terahertz light source and terahertz detector and sample perspective board up and down, and light source hole is correspondingly provided in the metal baffle；Sample perspective board uses cyclic olefine copolymer material, improves accuracy of detection, single unit system is simple in structure, easy to operation；The method of the present invention for carrying out cell count using the device carries out cell count by dripping sample, spectral detection and analytical procedure, and accuracy is high, and detection cycle is short, easy to operate.
Description
Technical field
The invention belongs to test or analyze biological substance technology by means of measuring the chemically or physically property of biological substance
Field is related to a kind of device and method carrying out cell count based on terahertz time-domain spectroscopic technology.
Background technology
In cell cultivation process, cell count is an essential step.Traditional method for cell count is using meter
Number plate is calculated since this method is that cell is diluted to lower concentration by formula by artificial counting under the microscope
Go out, certain error can be caused, simultaneously because human factor also brings along some subjective errors.
Strong absorption due to tera-hertz spectra to water, it is possible to which the tera-hertz spectra by acquiring cell suspension is believed
Breath establishes the Terahertz of standard first by calculating the terahertz light spectral absorption coefficient of the cell suspension of various concentration gradient
Spectral absorption curve；Then the tera-hertz spectra for acquiring tested cell suspension, cell sample can be carried out by standard curve
This counting.This method is simple to operate, time-consuming short, and subjective factor is not present, therefore error is small, and it is accurate to calculate.
Invention content
In view of this, carrying out cell count based on terahertz time-domain spectroscopic technology the purpose of the present invention is to provide a kind of
Device and method carries out the cell count of various types of cells system by the detection feature of tera-hertz spectra, is cell seeding and passage etc.
Reference is provided.
In order to achieve the above objectives, the present invention provides the following technical solutions：
It is a kind of to carry out the device of cell count based on terahertz time-domain spectroscopic technology, including holder, it is correspondingly arranged at holder
On Terahertz light source and terahertz detector, be distributed up and down and the gold between Terahertz light source and terahertz detector be set
Belong to baffle and sample perspective board, light source hole is correspondingly provided in the metal baffle；
The sample perspective board includes substrate and the cover board that is covered on substrate, and the substrate is equipped with for placing detection
The groove of sample；The shackle for placing sample perspective board is additionally provided on the holder.
Further, the substrate and cover board are using circular transparent sheet made of cyclic olefine copolymer material.
Further, a diameter of 50-100mm of the substrate, thickness 0.1-2mm, a diameter of 50- of the cover board
100mm, thickness 0.05-1mm.
Further, for cell suspension to be added dropwise in the groove of the substrate, the cell suspension refers to that cell is equal
It is dispersed in evenly in PBS buffer solutions, the volume of suspension is 7 μ L-1155 μ L；A diameter of 30-70mm of groove, thickness are
0.01-0.3mm。
Further, transparent outer cover is additionally provided with outside device.
The present invention also provides a kind of methods carrying out cell count based on terahertz time-domain spectroscopic technology：Including following step
Suddenly：
A, sample is dripped：Cell suspension is instilled into sample groove；
B, it detects：The time-domain spectroscopy information that cell suspension is acquired using terahertz light spectrum detection device, passes through sample cell
Thickness information seek the absorption coefficient of the sample；
C, it analyzes：The cell count curve that the absorption coefficient is substituted into the cell line, calculates the cell number of the sample.
Further, the cell count curve in the step c is to carry out concentration gradient spectral detection to the cell line of sample
The standard absorption coefficient curve of acquisition.
The beneficial effects of the present invention are：1) substrate and cover board for constituting sample perspective board use cyclic olefine copolymer material
It is made, there is low Terahertz to absorb, low-refraction, high transparency feature, better than existing by material preparations such as quartz, polyethylene
For tera-hertz spectra detection sample arrangement for perspective, improve accuracy of detection；2) utilize the unique water of tera-hertz spectra strong
Absorption characteristic carries out cell count, easy to operate, and accuracy is high, shortens detection cycle, is better than existing detection method.
Description of the drawings
In order to keep the purpose of the present invention, technical solution and advantageous effect clearer, the present invention provides following attached drawing and carries out
Explanation：
Fig. 1 is the structural diagram of the present invention；
Fig. 2 is the stereogram of terahertz light spectrum detection device.
Specific implementation mode
Below in conjunction with attached drawing, the preferred embodiment of the present invention is described in detail.
As shown in Figure 1, the device that cell count is carried out using tera-hertz spectra described in the present embodiment, including holder,
The Terahertz light source and terahertz detector that are correspondingly arranged on holder are distributed and are arranged in Terahertz light source and Terahertz up and down
Metal baffle between detector and sample perspective board are correspondingly provided with light source hole in the metal baffle.
In the present embodiment, Terahertz light source, metal baffle, sample perspective board and terahertz detector are set successively from top to bottom
It sets, sample perspective board therein is located on a shackle, shackle and Terahertz light source, metal baffle and terahertz detector
Equally it is each attached on holder；Metal baffle therein is mainly blocking Terahertz light source, prevents thz beam to surrounding
Scattering, therefore can be any metal materials such as copper, iron, aluminium, magnesium, use copper material in the present embodiment.
Sample perspective board in the present embodiment includes substrate and the cover board that is covered on substrate, and the substrate is equipped with and is used for
Place the groove of detection sample；When operation, measuring samples are placed in groove, cover cover board, it is easy to operate.
In the present embodiment, the substrate is thin using circular non-opaque made of cyclic olefine copolymer material with cover board
Plate, a diameter of 55mm of substrate, thickness 0.1mm, a diameter of 55mm of cover board, thickness 0.05mm；Cyclic olefine copolymer (letter
Claim COPs (Cyclo Olefin Polymers) or COCs (Cyclo Olefin Copolymers)) there is low Terahertz to inhale
It receives, the advantageous property of low-refraction and high transparency, be suitable as sample bearing device or detect the arrangement for perspective of sample, it can
Reduce carrying material in detection process to the influence factor of testing result.
If a diameter of 100mm of substrate, when thickness is 2mm, a diameter of 100mm, thickness 1mm can be selected in corresponding cover board
Such specification；Certainly, in the case where meeting hardness requirement, the thickness of substrate and cover board should be arranged relatively thin as possible, work as base
A diameter of 90mm of plate, thickness 1.3mm, a diameter of 90mm of corresponding cover board, thickness can reach when being 0.6mm needed for detection
Hardness requirement when, the substrate and cover board of this kind of slim specification should be selected as possible.
In the present embodiment, for cell suspension to be added dropwise in the groove of substrate, the volume of the cell suspension is in 7 μ
Between L-1155 μ L, groove diameter size is between 30-70mm, and thickness is between 0.01-0.3mm, specific cell suspension
Volume selected according to the actual conditions of detection, wherein the dropwise addition of the volume of cell suspension should be with the groove ruler on substrate
Very little corresponding, when the amount of cell suspension is more, the diameter of substrate is also answered larger, and thickness also should be thicker.
It is additionally provided with transparent glass outer in the present embodiment, outside detection device, ensures to realize constant humidity inside device, avoid
Because air flowing has an impact measurement, certainly, transparent plastic housing equally can reach this purpose.
The present invention also provides a kind of methods carrying out cell count using terahertz light spectrum detection device, including following step
Suddenly：
A, sample is dripped：Cell suspension is dropped in the groove of substrate, cover board is covered；
B, it detects：The terahertz detector acquisition opened Terahertz light source irradiating cell suspension, while be arranged below
The spectral information of cell suspension passes through the absorption coefficient of the THICKNESS CALCULATION of the groove cell suspension；
C, it analyzes：The cell count curve that the absorption coefficient is substituted into the cell line, calculates the cell number of the sample, wherein
Standard cell counts curve be the standard absorption coefficient curve that concentration gradient spectral detection acquisition is carried out to the cell line of sample,
Process is simple, and accuracy is high.
Finally illustrate, preferred embodiment above is merely illustrative of the technical solution of the present invention and unrestricted, although logical
It crosses above preferred embodiment the present invention is described in detail, however, those skilled in the art should understand that, can be
Various changes are made to it in form and in details, without departing from claims of the present invention limited range.
Claims (2)
1. a kind of device carrying out cell count based on terahertz time-domain spectroscopic technology, it is characterised in that：It is set including holder, correspondence
Terahertz light source and terahertz detector on holder are set, is distributed and is arranged in Terahertz light source and terahertz detector up and down
Between metal baffle and sample perspective board, be correspondingly provided with light source hole in the metal baffle；
The sample perspective board includes substrate and the cover board that is covered on substrate, and the substrate is equipped with for placing detection sample
Groove；
The shackle for placing sample perspective board is additionally provided on the holder；
The substrate and cover board are using circular transparent sheet made of cyclic olefine copolymer material；
A diameter of 50-100mm of the substrate, thickness 0.1-2mm, a diameter of 50-100mm of the cover board, thickness are
0.05-1mm；
For cell suspension to be added dropwise in the groove of the substrate, the cell suspension refers to being evenly dispersed in cell
In PBS buffer solutions, the volume of suspension is 7 μ L-1155 μ L；A diameter of 30-70mm of groove, thickness 0.01-0.3mm；
Transparent outer cover is additionally provided with outside device.
2. a kind of method carrying out cell count using claim 1 described device, it is characterised in that：Include the following steps：
A, sample is dripped：Cell suspension is instilled into sample groove；
B, it detects：The time-domain spectroscopy information that cell suspension is acquired using terahertz light spectrum detection device, passes through sample groove
Thickness information seeks the absorption coefficient of cell suspension；
C, it analyzes：The cell count curve that the absorption coefficient is substituted into the cell line of the cell suspension, it is suspended to calculate the cell
The cell number of liquid；
Cell count curve in the step c is to carry out concentration gradient spectral detection acquisition to the cell line of cell suspension
Standard absorption coefficient curve.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610061076.4A CN105486625B (en) | 2016-01-28 | 2016-01-28 | The device and method of cell count is carried out based on terahertz time-domain spectroscopic technology |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610061076.4A CN105486625B (en) | 2016-01-28 | 2016-01-28 | The device and method of cell count is carried out based on terahertz time-domain spectroscopic technology |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105486625A CN105486625A (en) | 2016-04-13 |
| CN105486625B true CN105486625B (en) | 2018-08-21 |
Family
ID=55673750
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610061076.4A Expired - Fee Related CN105486625B (en) | 2016-01-28 | 2016-01-28 | The device and method of cell count is carried out based on terahertz time-domain spectroscopic technology |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105486625B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111551514A (en) * | 2020-05-08 | 2020-08-18 | 南京大学 | High-sensitivity terahertz sensor capable of detecting trace cells and detection method |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106092952B (en) * | 2016-05-30 | 2019-03-22 | 成都曙光光纤网络有限责任公司 | A kind of central shielding device |
| CN108535219B (en) * | 2018-04-08 | 2021-05-14 | 北京环境特性研究所 | Tool device for measuring reflectivity and method for manufacturing sample for measuring reflectivity |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103335976A (en) * | 2013-06-04 | 2013-10-02 | 中国石油大学(北京) | Method for measuring concentrations of sulfate, nitrate solutions by utilizing terahertz time-domain spectroscopy |
| CN103616337A (en) * | 2013-12-18 | 2014-03-05 | 重庆绿色智能技术研究院 | Device and method for detecting bacteria in PM (Particulate Matter) 2.5 by utilizing terahertz spectrum |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2012185151A (en) * | 2011-02-17 | 2012-09-27 | Arkray Inc | Terahertz wave characteristic measuring method, substance detection method, measuring tool, terahertz wave characteristic measuring device and substance detection device |
| CN102305767B (en) * | 2011-05-17 | 2012-11-14 | 中国计量学院 | Microcontroller-based terahertz time-domain spectroscopy automatic sample testing device |
| CN102706804A (en) * | 2012-05-23 | 2012-10-03 | 中国科学院上海应用物理研究所 | Liquid sample cell |
| CN203502413U (en) * | 2013-10-28 | 2014-03-26 | 中国人民解放军第三军医大学 | Biological sample detection exposure system based on terahertz |
| CN103792204A (en) * | 2014-01-20 | 2014-05-14 | 郑州市中食农产品加工研究院 | Rapid microbiological detection technology based on terahertz time-domain spectroscopy |
-
2016
- 2016-01-28 CN CN201610061076.4A patent/CN105486625B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103335976A (en) * | 2013-06-04 | 2013-10-02 | 中国石油大学(北京) | Method for measuring concentrations of sulfate, nitrate solutions by utilizing terahertz time-domain spectroscopy |
| CN103616337A (en) * | 2013-12-18 | 2014-03-05 | 重庆绿色智能技术研究院 | Device and method for detecting bacteria in PM (Particulate Matter) 2.5 by utilizing terahertz spectrum |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111551514A (en) * | 2020-05-08 | 2020-08-18 | 南京大学 | High-sensitivity terahertz sensor capable of detecting trace cells and detection method |
Also Published As
| Publication number | Publication date |
|---|---|
| CN105486625A (en) | 2016-04-13 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103487396B (en) | Near infrared sugar degree the cannot-harm-detection device that a kind of illumination parameter is adjustable | |
| CN105486625B (en) | The device and method of cell count is carried out based on terahertz time-domain spectroscopic technology | |
| CN103063573B (en) | For the aperture system of multi-angle light diffusion detector | |
| CN103471982A (en) | Blood cell analysis chip, analysis meter and analysis method | |
| CN105277497A (en) | Device and method for detecting optical property parameters of agricultural products in continuous spectrum | |
| CN207147973U (en) | Heavy metal in water enrichment and measurement apparatus based on LIBS | |
| CN104833620B (en) | A kind of monitoring device of atmosphere particle concentration | |
| CN109239117A (en) | Directly measure trace Al, silicon, phosphorus, sulphur, the analytical equipment of chlorinity and method in sample | |
| CN109752363A (en) | The remaining Portable Raman optical spectrum detection method of pesticide in a kind of instant tea powder | |
| CN202735311U (en) | Test device for determining formaldehyde adsorption and degradation property | |
| CN204594848U (en) | A kind of monitoring device of atmosphere particle concentration | |
| CN104792686A (en) | Method for detecting microbe quantity and drug content of semisolid preparation through near infrared spectroscopy | |
| CN204287045U (en) | A kind of semi-integral ball fluorescence quantum efficiency measurement mechanism without optical baffle | |
| Zhao et al. | Measurement methods of single cell drug response | |
| CN204405548U (en) | A kind of portable SERS detects dull and stereotyped | |
| CN204008454U (en) | Portable near infrared spectrometer for detection of mould index in storage paddy | |
| CN207488183U (en) | Near-infrared spectrometer and transmission platform | |
| CN103185696B (en) | Solid sample carrying stage, spectrophotometer and LSPR (localized surface plasma resonance) detection method | |
| CN202512053U (en) | Optical system of large flat sheet spectrum tester | |
| CN103616337B (en) | Tera-hertz spectra is utilized to detect the device and method of bacterium in PM2.5 | |
| CN211374503U (en) | Spectrum detection device | |
| CN209530923U (en) | A kind of specimen holder for sample detection | |
| CN106092989A (en) | A kind of method by counting the quantum dot quantitative heparin of reunion ratio | |
| CN107247014B (en) | Device and method for measuring particle size distribution of vacuum intermediate infrared interference smoke screen | |
| CN2874483Y (en) | X-fluorescence sulfur detector |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20180821 Termination date: 20220128 |