CN105456406A

CN105456406A - Medicinal composition capable of improving autoimmunity of poultry

Info

Publication number: CN105456406A
Application number: CN201410461966.5A
Authority: CN
Inventors: 付春香; 张晓鹏; 吕晓静
Original assignee: Tianjin Ringpu Bio Technology Co Ltd
Current assignee: Tianjin Ringpu Bio Technology Co Ltd
Priority date: 2014-09-12
Filing date: 2014-09-12
Publication date: 2016-04-06

Abstract

The invention discloses a medicinal composition capable of improving the autoimmunity of poultry. The composition is prepared from fructus jujubae, fructus hippophae, rhizoma atractylodis macrocephalae, radix isatidis and poria cocos. Clinical experiments prove that the composition can effectively improve the autoimmunity of the poultry.

Description

A kind of pharmaceutical composition improving birds autoimmunity

Technical field

The present invention relates to a kind of pharmaceutical composition improving birds autoimmunity.

Background technology

China's aquaculture is the maximum field of antibiotic usage amount, exceedes the half of domestic antibiotic total quantity consumed.Domestic antibiotic total output 210,000 tons in 2006, domestic consumption amount about 180,000 tons, wherein, for the antibiotic of herding and feedstuff industry just up to 9.7 ten thousand tons, accounts for 54%.In aquaculture, antibiotic use is mainly divided into two large divisions.A part is added for Feed Enterprise in the process of production full price, concentrated, premix material, is mainly used in prevention epidemic disease and growth promoting effects.Another part uses in breeding process for raiser, and adopt spice, drinking-water, inject, gavage and the various ways such as environment sprinkling, application target mostly is prevention and therapy livestock and poultry pestilence.In China, because cultivation density is large, complicated various the adding of livestock and poultry pestilence supervises the many reasons such as ineffective, the excessive use of ubiquity antibiotic such as even to abuse at the problem, this causes food-safety problem increasingly serious, and the improving gradually also for the sustainable and healthy development of aquaculture hides some dangers for of bacterial drug resistance.Along with various antibiotic use, poultry are faced with the threat of the stronger antibacterial of drug resistance and virus, and wherein exploitation improves the important directions that poultry autoimmunity is prevention Animal diseases.Current world wide is interior is the vaccine injection to birds of medical category and the extreme final hit measure to birds in source of disease point 50 kilometers to 300 kilometer range to the mode of the pre-anti-virus of birds.Although this mode is very effective, comparatively passive, cause huge waste, and operating process is too complicated.

Disclose kind of the pharmaceutical composition improving birds autoimmunity for such situation the present invention, composition of medicine is made up of Fructus Jujubae, Fructus Hippophae, the Rhizoma Atractylodis Macrocephalae, Poria, Radix Isatidis etc.Wherein Fructus Jujubae not only has the proliferation function that immunosuppressive action also has anticancer, and Poria has antibacterial action.Prove through clinical trial, this prescription is effective, simple to operate; Meeting outside the edible required nutrition of birds, the epidemic disease that effectively can also reduce Animal by-product is polluted and the residual problem of medicine, improve Animal by-product quality and can not produce drug resistance, avoid the possibility of abuse, overcome the shortcoming that antibiotic self is intrinsic well, and cheap, large burden can not be caused to chicken house.

Summary of the invention

The object of this invention is to provide a kind of pharmaceutical composition improving birds autoimmunity.

Described compositions comprises Fructus Jujubae, Fructus Hippophae, the Rhizoma Atractylodis Macrocephalae, Radix Isatidis, Poria

The formula of described compositions is: Fructus Jujubae 6-15g, Fructus Hippophae 1-3g, Rhizoma Atractylodis Macrocephalae 6-12g, Radix Isatidis 9-15g, Poria 9-15g.

The formula of described compositions is preferably: Fructus Jujubae 12g, Fructus Hippophae 2g, Rhizoma Atractylodis Macrocephalae 8g, Radix Isatidis 11g, Poria 12g.

Described compositions is completed by following preparation technology:

(1) take each component of compositions by embodiment 1 recipe quantity, decoct with water twice, for the first time 360L water, second time 270L water, keep micro-at every turn after boiling and boil 2 hours, 200 mesh filter screens filter while hot.Merge twice filtrate, mixing, is concentrated into the relative density 1.1-1.2 of medicinal liquid, and adding ethanol to the concentration of ethanol is 30%, cold preservation 24 hours, filters, filtrate recycling ethanol, be concentrated into the relative density 1.1-1.2 of medicinal liquid, add water and be settled to 45L, every milliliter is equivalent to crude drug in whole 1g.

(2) take each component of compositions by embodiment 2 recipe quantity, decoct with water twice, for the first time 248L water, second time 186L water, keep micro-at every turn after boiling and boil 2 hours, 200 mesh filter screens filter while hot.Merge twice filtrate, mixing, is concentrated into the relative density 1.1-1.2 of medicinal liquid, and adding ethanol to the concentration of ethanol is 30%, cold preservation 24 hours, filters, filtrate recycling ethanol, be concentrated into the relative density 1.1-1.2 of medicinal liquid, add water and be settled to 31L, every milliliter is equivalent to crude drug in whole 1g.

(3) take each component of compositions by embodiment 3 recipe quantity, decoct with water twice, for the first time 480L water, second time 360L water, keep micro-at every turn after boiling and boil 2 hours, 200 mesh filter screens filter while hot.Merge twice filtrate, mixing, is concentrated into the relative density 1.1-1.2 of medicinal liquid, and adding ethanol to the concentration of ethanol is 30%, cold preservation 24 hours, filters, filtrate recycling ethanol, be concentrated into the relative density 1.1-1.2 of medicinal liquid, add water and be settled to 60L, every milliliter is equivalent to crude drug in whole 1g.

Beneficial effect of the present invention:

This invention compatibility rationally, have no side effect, can improve immunity of organisms, and prepare simply compared with the medicine used on a large scale with present chicken house, cheap, drug residue free, has good economic worth, can industrial application.

Detailed description of the invention:

Embodiment 1

Embodiment 2

Embodiment 3

Preparation method:

(1) take each component of compositions by recipe quantity, decoct with water twice, for the first time 360L water, second time 270L water, keep micro-at every turn after boiling and boil 2 hours, 200 mesh filter screens filter while hot.Merge twice filtrate, mixing, is concentrated into the relative density 1.1-1.2 of medicinal liquid, and adding ethanol to the concentration of ethanol is 30%, cold preservation 24 hours, filters, filtrate recycling ethanol, be concentrated into the relative density 1.1-1.2 of medicinal liquid, add water and be settled to 45L, every milliliter is equivalent to crude drug in whole 1g.

(2) take each component of compositions by recipe quantity, decoct with water twice, for the first time 248L water, second time 186L water, keep micro-at every turn after boiling and boil 2 hours, 200 mesh filter screens filter while hot.Merge twice filtrate, mixing, is concentrated into the relative density 1.1-1.2 of medicinal liquid, and adding ethanol to the concentration of ethanol is 30%, cold preservation 24 hours, filters, filtrate recycling ethanol, be concentrated into the relative density 1.1-1.2 of medicinal liquid, add water and be settled to 31L, every milliliter is equivalent to crude drug in whole 1g.

(3) take each component of compositions by recipe quantity, decoct with water twice, for the first time 480L water, second time 360L water, keep micro-at every turn after boiling and boil 2 hours, 200 mesh filter screens filter while hot.Merge twice filtrate, mixing, is concentrated into the relative density 1.1-1.2 of medicinal liquid, and adding ethanol to the concentration of ethanol is 30%, cold preservation 24 hours, filters, filtrate recycling ethanol, be concentrated into the relative density 1.1-1.2 of medicinal liquid, add water and be settled to 60L, every milliliter is equivalent to crude drug in whole 1g.

Embodiment 4 present composition is to the clinical trial of poultry immunity:

1. experiment material

The compositions of the proportions in 1.1 employing embodiments 1

1.2 select healthy, uniform 7 aa broiler chicken 120 of body weight, and measure a kind of feedstuff at random

The astragalus polysaccharides powder that 1.3 markets are bought

2. method

Only 3 groups are divided at random to 7 aa broiler chicken 120, grouping raising 42 days.

First group is blank group, and feedstuff does not add Chinese medicine of the present invention.

Second group, as drug control group, is added the astragalus polysaccharides powder that market is bought in feedstuff.

3rd group is experimental group, and in drink feedstuff, add Chinese medicine of the present invention, addition is the ratio of 1:100.

In addition, the rearing conditions of two groups controls identical, and duration of test observes situation of searching for food and the mental status of chicken every day, within after last administration 24 hours, often organizes to cut open at random and kills 10 calculating organ indexs.

Table one: the impact of chicken daily gain [g/ (d plumage):

	Blank	Drug control group	Experimental group
				Average daily gain g	9656	9835	11024
Average daily ingestion amount g	29954	29991	29969
				Feed-weight ratio %	3.1	3.0	2.7

Add the body weight that Chinese medicine of the present invention significantly can increase broiler as shown in Table 1 in feedstuff, feed-weight ratio can be reduced improve efficiency of feed utilization compared with matched group.

Table two: medicine is on the impact of the organ index of Immune Organs of Chicken;

	Blank	Positive control	Medicine group
				Thymus index	3.57±0.21	3.85±0.12	4.01±0.15*
Spleen index	1.01±1.08	1.35±1.18	1.51±1.21**
				Bursal index	1.66±0.09	1.96±0.18	2.12±0.19**

Note: *: p < 0.05, * *: p < 0.01

Administration group as shown in Table 2 can significantly increase each organ index compared with matched group and medicine group is comparatively comparatively remarkable positive controls.

Table three: the mortality rate of chicken;

Group	Chicken number (only)	Death toll (only)	Mortality rate (%)
				Blank	40	3	7.5
Positive control	40	1	2.5
				Medicine group	40	0	0.0

As can be seen from table three, testing the full phase does not have death except administration group chicken, and all the other 2 groups have all occurred death, illustrate that this invention Chinese medicine has certain effect to chicken control and prevention of disease, can improve the immunity of chicken.

As can be seen from above experiment, after edible Chinese medicine of the present invention, the probability that chicken catches reduces, mortality rate is all reduced to very low level, illustrate that Chinese medicine of the present invention can improve beasts immunity, improve resistance against diseases, greatly reduce the cost of raising like this, increasing income, is a technology be worthy to be popularized.

Claims

1. improve a pharmaceutical composition for birds autoimmunity, it is characterized in that, described compositions comprises Fructus Jujubae, Fructus Hippophae, the Rhizoma Atractylodis Macrocephalae, Radix Isatidis, Poria.

2. a kind of pharmaceutical composition improving birds autoimmunity according to claim 1, is characterized in that the formula of described compositions is: Fructus Jujubae 6-15g, Fructus Hippophae 1-3g, Rhizoma Atractylodis Macrocephalae 6-12g, Radix Isatidis 9-15g, Poria 9-15g.

3. a kind of pharmaceutical composition improving birds autoimmunity according to claim 2, is characterized in that described composite formula is Fructus Jujubae 12g, Fructus Hippophae 2g, Rhizoma Atractylodis Macrocephalae 8g, Radix Isatidis 11g, Poria 12g.

4. improve a pharmaceutical composition for birds autoimmunity, it is characterized in that being completed by following preparation technology:

Take each component of compositions by recipe quantity, decoct with water twice, for the first time 8 times of water, second time 6 times of water, keep micro-at every turn and boil 2 hours after boiling, 200 mesh filter screens filter while hot; Merge twice filtrate, mixing, is concentrated into the relative density 1.1-1.2 of medicinal liquid, adding ethanol to the concentration of ethanol is 30%, cold preservation 24 hours, filters, filtrate recycling ethanol, is concentrated into the relative density 1.1-1.2 of medicinal liquid, adds water to be settled to every milliliter and to be equivalent to crude drug in whole 1g.