CN105456086A - Preparation method and application of rosa canina fruit extract - Google Patents
Preparation method and application of rosa canina fruit extract Download PDFInfo
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- CN105456086A CN105456086A CN201510740602.5A CN201510740602A CN105456086A CN 105456086 A CN105456086 A CN 105456086A CN 201510740602 A CN201510740602 A CN 201510740602A CN 105456086 A CN105456086 A CN 105456086A
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- dog rose
- preparation
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- berry extract
- biological enzyme
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
Abstract
The invention relates to the field of daily chemical, in particular to a preparation method of a rosa canina fruit extract and application thereof in a skin care cream. The preparation method mainly comprises the steps that 1, dried rosa canina fruits are subjected to superfine grinding so as to pass through a 100-mesh sieve; 2, a composite biological enzyme is prepared; 3, the ground rosa canina fruits are added into a solvent, then the temperature is increased to 50-55 DEG C, then the composite biological enzyme is added, the pH is adjusted by acid to be 4.5-5.0, after the mixture is stirred to be uniform, enzymolysis is kept for 2 H at the temperature of 50-55 DEG C, then the temperature is rapidly increased to 100 DEG C for rapid inactivation, the temperature is decreased to 80 DEG C 1 MIN after inactivation, and reflux extraction is carried out for 1H; 4, the extract is filtered, deslagged, concentrated and dried to obtain an educt. The method has the advantages of being high in catalysis efficiency, mild in catalysis condition, and capable of lowering energy consumption, reducing pollution and simplifying technological procedures. When the rosa canina fruit extract prepared through the preparation method is applied to the skin care cream, the effects of well moistening, moisturizing and repairing skin can be achieved.
Description
Technical field
The present invention relates to the extraction field of natural product, be specifically related to a kind of preparation method of dog rose berry extract and the application in cold cream frost thereof.
Background technology
Dog rose fruit is Rosehips again, is the one in numerous Flos Rosae Rugosae or Flos Rosae Multiflorae plant, contains Caulis et Folium Lini, oleic acid is very important to the health of skin in Fructus Rosae.Undersaturated linolenic acid and linoleic acid can and protein be combined with cholesterol, to form the film alive of getting together with cell membrane, make the rapid granulating of skin and regeneration.
The Vitamin C content of dog rose fruit is very high, be every hectogram fresh fruit edible part VC content more than 6810 milligrams after testing, reach as high as 8300 milligrams, occupy first of all veterinary antibiotics, that " hat of the earth fruit " is known as " king of VC ", due to the vitamin C of its high-load, also honor has determined it in the extensive use on boundary of improving looks.From the essence substance that dog rose fruit is extracted, can promote the ability of the new cell of skin hyperplasia, along with new cell replaces aging cell, pigmentation speckle will alleviate gradually.
Dog rose fruit elite also has that moistness is strong, the double grading of high osmosis, can maintain the elasticity of all cells and moist, wakes skin regeneration ability up and have efficient moisture-keeping efficacy, and thus it is application very extensive of cosmetic field.
Research surface, with advancing age, skin aging, the barrier function of skin weakens gradually.In addition the impact of environment increases the weight of day by day, people to antioxidation, Skin Cell hypertrophy can be promoted, the cosmetics demand of skin regeneration ability can be waken up day by day increase.And as the representational dog rose of most skim the cream off milk improve skin barrier function, maintain cell elasticity and moist in application have good market prospect.
In the prior art, have a lot from the preparation method of dog rose berry extract, as supercritical fluid extraction, ultrasonic extraction, microwave loss mechanisms, leaching method etc.And these methods to there is extraction efficiency low, the problems such as technique is comparatively complicated, and extraction ratio is lower, and extraction cost is high, and environmental pollution is larger.
Summary of the invention
Technical problem to be solved by this invention is to provide a kind of preparation method and the application of this extract in cold cream frost of dog rose berry extract.
Technical problem to be solved by this invention is achieved by the following technical programs:
A preparation method for dog rose berry extract, comprises following steps:
(1) get the dog rose fruit of oven dry, micronizing can cross 100 mesh sieves.
(2) prepare compound biological enzyme, the active unit of enzyme is 500,000 U/g
(3) the dog rose fruit powder after pulverizing is added deionized water, then temperature is risen to 50 ~ 55 DEG C, then compound biological enzyme is added, be 4.5 ~ 5.0 with acid for adjusting pH, after stirring, keep 50 ~ 55 DEG C of enzymolysis 2H, then rapid temperature increases to 100 DEG C carries out quick inactivating, is cooled to 80 DEG C of reflux, extract, 1H. after deactivation 1MIN
(4) extract carried out filter, remove slag, concentrated and dry extract.
Preferably, described compound biological enzyme presses 20:5:6:3 proportions by cellulase, amylase, pectase, protease.
Preferably, solvent described in step 3 is the mixture of one or more in water, ethanol, methanol, acetone, petroleum ether, normal hexane.
Preferably, described dog rose fruit powder and the feed weight of Extraction solvent are than being 1:3 ~ 1:8.
Preferably, the consumption of described compound biological enzyme is 0.5% ~ 1% of Fructus Rosae powder.
Preferably, the concentrate drying of described step (4) described extract is treated to extracting the dog rose fruit extracting solution input point flow container obtained, control evaporating temperature between 55-65 DEG C, air pressure controls to make extracting solution evaporation and concentration to the 25-45% of extracting solution total amount at-0.1-0Mpa, obtains dog rose berry extract after continuing drying under reduced pressure 8h.
The dog rose berry extract that the present invention adopts enzyme-circumfluence method to extract, by enzyme preparation catalysis, usefulness is high, mild condition, remains the both effectiveness of dog rose fruit in maximum efficiency.Extract essence is rich in vitamin C, the ability of the new cell of skin hyperplasia can be promoted, have that moistness is strong, the double grading of high osmosis, the elasticity of all cells and moist can be maintained, thus be widely used at cosmetic field, thing of the present invention is applied in cold cream frost.
An efficient moist preserving moisture and protecting skin cream frost, comprises following component by weight percentage:
The wetting agent of 0.1 ~ 30.0%; The emulsifying agent of 0.1 ~ 8%; The emollient of 0.1 ~ 30%; 0.1-10% dog rose berry extract; The stable thickening agent of 0.1 ~ 2%; The additive of 0.01 ~ 3%; Antiseptic≤1.0%; And be supplemented to 100% with water.
Wherein, preferably, described wetting agent is selected from the mixture of one or more in 1,3 butylene glycol, propylene glycol, glycerol, betanin, Mel nature moisturizing factor.Preferred, be the mixture of one or more in the betanin of natural origin and 1,3 butylene glycol, Mel nature moisturizing factor, glycerol.Betanin is extracted from beet root completely, and containing 99% active matter, pure gentleness, except giving moisturizing performance, can tackle oxidation stimulation and UV damages equally, in addition can also the synthesis of stimulation collagen, makes skin flexible.
Preferably, described emulsifying agent be selected from tristerin (with) PEG-100 stearate, cetyl glucoside, ceteareth-20, one or several mixture in soybean lecithin, be more preferably above 2 kinds and arrange in pairs or groups used in combination in proportion.
Preferably, described emollient is hexadecanol, octadecanol, cetostearyl alcohol, dimethicone, isopropyl palmitate, Jojoba oil, cera alba, lanoline, vaseline, one or several the mixture in shea butter.Be more preferably shea butter and be mixed in proportion use with other oils and fatss.
Preferably, described stable thickening agent is selected from the mixture of one or more in acrylate copolymer, hydroxyethyl-cellulose, xanthan gum, hydroxy ethyl methacrylate/sodium acryloyldimethyl taurate copolymers.
Preferably, described additive is selected from least two kinds in plant sterol, oil-soluble Flos Inulae extract, Marine fish collagen and argan oil.Plant sterol has very high permeability to skin, can keep skin surface moisture content, promotes skin metabolism, suppresses scytitis, can anti-sunburn, skin aging, has the characteristics such as good skinfeel (spreadability is good, smooth does not glue).
Flos Inulae grows all over the world as garden plant, is one of the most useful a kind of medical draft.Having effect of the anticorrosion and anti-inflammatory of nature, is one of the best draft at treatment wound, skin abrasion place.Make an addition in cosmetics and can alleviate skin trauma, sunburn, and the cells grown of new skin can be stimulated.
Marine fish collagen is a kind of collagen protein of natural origin, this albumen is found to be the major protein (being greater than 75%) in conjunctive tissue and skin, it maintain play a part in the tone of skin, softness and elasticity very important, and there is extremely low molecular weight, must aminoacid containing the 8 kinds of human bodies accounting for all total amino acid contents 14.5%.
Argan oil contains abundant fatty acid, sterol, vitamin e and phenolic compound.Comprising a large amount of essential fatty acid, unsaturated fatty acid and oleum lini, these be all can not synthesize in human body and must get from fat.And polyphenol is well-known antioxidant, it can not only delay skin aging, can also extend other antioxidant in human body, as vitamin E, ascorbic action time.
The present invention's antiseptic used is the conventional antiseptic of the art.
Applying biological enzyme-backflow preparation method of the present invention, the compact texture of cell wall is destroyed by enzyme, cause cell wall and intercellular substance structure to produce local to loosen, expand, the effective ingredient reducing cell wall and intercellular substance barrier from born of the same parents to the resistance to mass tranfer of Extraction medium diffusion, thus be conducive to effective ingredient stripping.It is high that the method also has catalytic efficiency, and catalytic condition is gentle, can reduce usefulness, decreasing pollution, the feature of Simplified flowsheet program.Natural for dog rose berry extract of the present invention, safety, it is added in cold cream frost, can plays and well moisten moisture-keeping efficacy, skin barrier function can also be improved, maintain the elasticity of cell and moisten.
Accompanying drawing explanation
Fig. 1 is extraction process flow chart of the present invention.
Detailed description of the invention
Below in conjunction with embodiment, the present invention will be described in detail, and embodiment is only the preferred embodiment of the present invention, is not limitation of the invention.
Embodiment 1
A preparation method for dog rose berry extract, comprises following steps:
(1) get the dog rose fruit of oven dry, micronizing can cross 100 mesh sieves.
(2) prepare compound biological enzyme, cellulase, amylase, pectase, protease press 20:5:6:3 proportions, and the active unit of enzyme is 500,000 U/g
(3) the dog rose fruit powder after pulverizing is added deionized water, then temperature is risen to 55 DEG C, then compound biological enzyme is added, be 4.5 with acid for adjusting pH, after stirring, keep 55 DEG C of enzymolysis 2H, then rapid temperature increases to 100 DEG C carries out quick inactivating, is cooled to 80 DEG C of reflux, extract, 1H. after deactivation 1MIN
(4) extract is carried out filtering, removing slag, extracting the Fructus Rosae extracting solution input point flow container obtained, control evaporating temperature between 65 DEG C, air pressure controls to make extracting solution evaporation and concentration to 30% of extracting solution total amount at-0.1Mpa, obtains dog rose fruit and skim the cream off milk after continuing drying under reduced pressure 8h.
Embodiment 2
A preparation method for dog rose berry extract, comprises following steps:
(1) get the dog rose fruit of oven dry, micronizing can cross 100 mesh sieves.
(2) prepare compound biological enzyme, cellulase, amylase, pectase, protease press 6:3:2:2 proportions, and the active unit of enzyme is 500,000 U/g
(3) the dog rose fruit powder after pulverizing is added ethanol, then temperature is risen to 50 DEG C, then compound biological enzyme is added, be 4.8 with acid for adjusting pH, after stirring, keep 50 DEG C of enzymolysis 2H, then rapid temperature increases to 100 DEG C carries out quick inactivating, is cooled to 80 DEG C of reflux, extract, 1H. after deactivation 1MIN
(4) extract is carried out filtering, removing slag, extracting the Fructus Rosae extracting solution input point flow container obtained, control evaporating temperature between 60 DEG C, air pressure controls to make extracting solution evaporation and concentration to 30% of extracting solution total amount at-0.1Mpa, obtains dog rose fruit and skim the cream off milk after continuing drying under reduced pressure 8h.
Embodiment 3
A preparation method for dog rose berry extract, comprises following steps:
(1) get the dog rose fruit of oven dry, micronizing can cross 100 mesh sieves.
(2) prepare compound biological enzyme, cellulase, amylase, pectase, protease press 4:1:1:2 proportions, and the active unit of enzyme is 500,000 U/g
(3) the dog rose fruit powder after pulverizing is added methanol, then temperature is risen to 55 DEG C, then compound biological enzyme is added, be 5.0 with acid for adjusting pH, after stirring, keep 50 DEG C of enzymolysis 2H, then rapid temperature increases to 100 DEG C carries out quick inactivating, is cooled to 80 DEG C of reflux, extract, 1H. after deactivation 1MIN
(4) extract is carried out filtering, removing slag, extracting the Fructus Rosae extracting solution input point flow container obtained, control evaporating temperature between 55 DEG C, air pressure controls to make extracting solution evaporation and concentration to 30% of extracting solution total amount at 0Mpa, obtains dog rose fruit and skim the cream off milk after continuing drying under reduced pressure 8h.
Embodiment 4
Moisten a moisturizing cream frost, comprise the component of following percentage by weight:
3% betanin, 5% glycerol, 2% cetyl glucoside, 1.5% tristerin (with) PEG-100 stearate, 2% shea butter, 3% isopropyl palmitate, 1.5% dimethicone, 1.5% Jojoba oil, 0.2% acrylate copolymer, 0.1%EDTA-2Na, 0.5% plant sterol, 0.2% Marine fish collagen, 1% embodiment dog rose extract, the triethanolamine of 0.2%, 0.8% phenoxyethanol, 0.02% iodo propinyl butyl carbamate, complements to 100% with water.
Embodiment 5
Moisten a moisturizing cream frost, comprise the component of following percentage by weight:
3% betanin, 5% glycerol, 2% cetyl glucoside, 1.5% tristerin (with) PEG-100 stearate, 2% shea butter, 3% isopropyl palmitate, 1.5% dimethicone, 1.5% Jojoba oil, 0.2% acrylate copolymer, 0.1%EDTA-2Na, 0.5% plant sterol, 0.2% Marine fish collagen, 3% embodiment dog rose extract, the triethanolamine of 0.2%, 0.8% phenoxyethanol, 0.02% iodo propinyl butyl carbamate, complements to 100% with water.
Embodiment 6
Moisten a moisturizing cream frost, comprise the component of following percentage by weight:
3% betanin, 5% glycerol, 2% cetyl glucoside, 1.5% tristerin (with) PEG-100 stearate, 2% shea butter, 3% isopropyl palmitate, 1.5% dimethicone, 1.5% Jojoba oil, 0.2% acrylate copolymer, 0.1%EDTA-2Na, 0.5% plant sterol, 0.2% Marine fish collagen, 5% embodiment dog rose extract, the triethanolamine of 0.2%, 0.8% phenoxyethanol, 0.02% iodo propinyl butyl carbamate, complements to 100% with water.
Comparative example
Moisten a moisturizing cream frost, comprise the component of following percentage by weight:
3% betanin, 5% glycerol, 2% cetyl glucoside, 1.5% tristerin (with) PEG-100 stearate, 2% shea butter, 3% isopropyl palmitate, 1.5% dimethicone, 1.5% Jojoba oil, 0.2% acrylate copolymer, 0.1%EDTA-2Na, 0.5% plant sterol, 0.2% Marine fish collagen, the triethanolamine of 0.2%, 0.8% phenoxyethanol, 0.02% iodo propinyl butyl carbamate, complements to 100% with water.
Preparation method of the present invention is conventional oil-water emulsion hybrid technology, does not elaborate at this.
Through contrast test, moisten moisturizing cream frost test process and the results are shown in Table one:
First drier volunteer totally 40 people of skin are found, every 5 people one group, totally 8 groups, use embodiment and comparative example totally four products respectively, use 10 days continuously, continue tracking again 5 days, evaluated its skin before use product every day by 10 skin experts, often group gets its meansigma methods, and (wherein 1-10 refers to skin health index to result such as following table one, index is higher, and skin is more healthy)
Table 1 volunteer is to moist moisturizing cream frost and sample for reference effect assessment mark
| Group | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 |
| Embodiment 4 | 6 | 7 | 8 | 8 | 6 | 8 | 8 | 6 |
| Embodiment 5 | 8 | 8 | 8.5 | 8.5 | 8 | 8.5 | 9 | 8 |
| Embodiment 6 | 10 | 9.5 | 8.5 | 9 | 9 | 9.5 | 9 | 9 |
| Comparative example | 5 | 4 | 5 | 5 | 2 | 4 | 5 | 5 |
Find out to use respectively have well moist moisturizing and repair containing 1%, 3%, 5%, 0% dog rose extract to skin from the experimental data of table one, very not little to skin repair effect containing dog rose extract.Moist moisturizing and reparation effect of containing 5% dog rose extract as can be seen from the table improve 2 times than common effect effect.
Claims (10)
1. a preparation method for dog rose berry extract, is characterized in that, comprises the steps:
(1) get the dog rose fruit of oven dry, micronizing can cross 100 mesh sieves;
(2) prepare compound biological enzyme, the active unit of enzyme is 500,000 U/g;
(3) the dog rose fruit powder after pulverizing is added in solvent, then temperature is risen to 50 ~ 55 DEG C, then compound biological enzyme is added, PH is regulated to be 4.5 ~ 5.0 with acid, after stirring, keep 50 ~ 55 DEG C of enzymolysis 2H, then rapid temperature increases to 100 DEG C carries out quick inactivating, is cooled to 80 DEG C of reflux, extract, 1H after deactivation 1MIN;
(4) extract carried out filter, remove slag, concentrated and dry extract.
2. the preparation method of a kind of dog rose berry extract according to claim 1, is characterized in that, described compound biological enzyme presses 20:5:6:3 proportions by cellulase, amylase, pectase, protease.
3. the preparation method of a kind of dog rose berry extract according to claim 1, is characterized in that, solvent described in step 3 is one or several the mixture in water, ethanol, methanol, acetone, petroleum ether, normal hexane.
4. the preparation method of a kind of dog rose berry extract according to claim 1, is characterized in that, described dog rose fruit powder is 1:3 ~ 1:8 with the feed weight ratio of solvent.
5. the preparation method of a kind of dog rose berry extract according to claim 1, is characterized in that, the consumption of described compound biological enzyme is 0.5% ~ 1% of dog rose fruit powder.
6. the preparation method of a kind of dog rose berry extract according to claim 1, it is characterized in that, the concentrate drying of step (4) described extract is treated to extracting the dog rose fruit extracting solution input point flow container obtained, control evaporating temperature between 55-65 DEG C, air pressure controls to make extracting solution evaporation and concentration to the 25-45% of extracting solution total amount at-0.1-0Mpa, obtains dog rose berry extract after continuing drying under reduced pressure 8h.
7. the application of dog rose berry extract in cold cream frost of the preparation method gained described in claim 1 ~ 6 any one.
8. a cold cream frost, is characterized in that the component comprising following percentage by weight:
The wetting agent of 0.1 ~ 30.0%;
The emulsifying agent of 0.1 ~ 8%;
The emollient of 0.1 ~ 30%;
0.1-10% dog rose berry extract;
The stable thickening agent of 0.1 ~ 2%;
The additive of 0.01 ~ 3%;
The antiseptic of≤1.0%
Surplus is water.
9. a kind of cold cream frost according to claim 8, is characterized in that: wetting agent is the mixture of one or more in 1,3 butylene glycol, propylene glycol, glycerol, betanin, Mel nature moisturizing factor.
10. a kind of cold cream frost according to claim 8, is characterized in that: described emulsifying agent be tristerin (with) PEG-100 stearate, cetyl glucoside, ceteareth-20, one or several mixture in soybean lecithin.
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| CN105997837A (en) * | 2016-06-17 | 2016-10-12 | 广州岚萃贸易有限公司 | Formula of spray for removing cutin |
| CN107375079A (en) * | 2017-08-04 | 2017-11-24 | 澳宝化妆品（惠州）有限公司 | A kind of extracting method of cacao seed extract and its application in hand frost |
| CN108379126A (en) * | 2018-04-26 | 2018-08-10 | 广东岭南职业技术学院 | A kind of fat of Oromaius norvaehollandeae cold cream and preparation method thereof |
| CN111903978A (en) * | 2020-07-02 | 2020-11-10 | 太阳树（厦门）生物工程有限公司 | Production method of rose hip extract containing VC |
| CN113316448A (en) * | 2018-12-20 | 2021-08-27 | Lvmh研究公司 | Rosa multiflora wood extract |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105997837A (en) * | 2016-06-17 | 2016-10-12 | 广州岚萃贸易有限公司 | Formula of spray for removing cutin |
| CN107375079A (en) * | 2017-08-04 | 2017-11-24 | 澳宝化妆品（惠州）有限公司 | A kind of extracting method of cacao seed extract and its application in hand frost |
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| CN113316448A (en) * | 2018-12-20 | 2021-08-27 | Lvmh研究公司 | Rosa multiflora wood extract |
| CN111903978A (en) * | 2020-07-02 | 2020-11-10 | 太阳树（厦门）生物工程有限公司 | Production method of rose hip extract containing VC |
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