CN105456086B - A kind of preparation method and applications of dog rose berry extract - Google Patents
A kind of preparation method and applications of dog rose berry extract Download PDFInfo
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- CN105456086B CN105456086B CN201510740602.5A CN201510740602A CN105456086B CN 105456086 B CN105456086 B CN 105456086B CN 201510740602 A CN201510740602 A CN 201510740602A CN 105456086 B CN105456086 B CN 105456086B
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
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Abstract
The present invention relates to household chemicals field, and in particular to a kind of preparation method of extract of dog rose fruit and its application in skin care cream.Wherein, the preparation method, mainly includes：(1) the dog rose fruit of drying is taken, ultramicro grinding can cross 100 mesh sieves；(2) compound biological enzyme is prepared；(3) the dog rose fruit powder after crushing is added in solvent, then temperature is risen to 50~55 DEG C, then compound biological enzyme is added, it is 4.5~5.0 with acid for adjusting pH, after stirring, 50~55 DEG C of enzymolysis 2H are kept, is then brought rapidly up to 100 DEG C of progress quick inactivatings, 80 DEG C of refluxing extraction 1H is cooled to after inactivating 1MIN；(4) extract filtered, removed slag, being concentrated and dry extract.The inventive method has high catalytic efficiency, and catalytic condition is gentle, can reduce efficiency, reduces pollution, the characteristics of simplifying process.The dog rose extract prepared using the present invention is applied in skin care cream, the effect of having moist moisturizing well and repair skin.
Description
Technical field
The present invention relates to the extraction field of natural products, and in particular to a kind of preparation method of dog rose berry extract and
Its application in skin care cream.
Background technology
Dog rose fruit is called fructus rosae, is one kind in numerous rose or rose plants, contains flax, oil in cynarrhodion
Acid is to the healthy particularly important of skin.Undersaturated leukotrienes and linoleic acid can and protein combined with cholesterol, with formation and
The work film that cell membrane is got together, makes skin granulate and regenerate rapidly.
The Vitamin C content of dog rose fruit is very high, is detected as every milli of hectogram fresh fruit edible part VC contents 6810
More than gram, reach as high as 8300 milligrams, occupy first of all veterinary antibiotics, be " hat of the earth fruit " be known as " VC it
King ", due to the vitamin C of its high content, also honor has determined its extensive use in beauty circle.From the elite of dog rose fruit extraction
Material, the ability of skin hyperplasia neoblast can be promoted, as new cell replaces the cell of aging, pigmentation spot will be by
It is decrescence light.
Dog rose fruit elite also has that moistness is strong, the double grading of high osmosis, can maintain the elasticity of all cells
And it is moist, wake up skin regeneration ability and there is efficient moisture-keeping efficacy, thus it is very wide in the application of cosmetic field
It is general.
Surface is studied, with advancing age, the aging of skin, the barrier function of skin gradually weakens.In addition environment
Influence increasingly aggravates, and people are to cosmetics demand day that is anti-oxidant, can promoting Skin Cell hyperplasia, can wake up skin regeneration ability
Benefit increase.And as most representational dog rose skim the cream off milk improve skin barrier function, maintain cell elasticity and
The application of moist aspect has good market prospects.
In the prior art, have much from the preparation method of dog rose berry extract, such as supercritical fluid extraction, surpass
Sound extraction method, microwave loss mechanisms, leaching method etc..And these methods have that extraction efficiency is low, and technique is complex, recovery rate compared with
It is low, the problems such as extraction cost is high, and environmental pollution is larger.
The content of the invention
The technical problems to be solved by the invention are to provide preparation method and extraction of a kind of dog rose berry extract
Application of the thing in skin care cream.
The technical problems to be solved by the invention are achieved by the following technical programs：
A kind of preparation method of dog rose berry extract, is comprised the following steps：
(1) the dog rose fruit of drying is taken, ultramicro grinding can cross 100 mesh sieves.
(2) compound biological enzyme is prepared, the active unit of enzyme is 500,000 U/g
(3) the dog rose fruit powder after crushing is added into deionized water, temperature is then risen to 50~55 DEG C, then added
Compound biological enzyme, with acid for adjusting pH be 4.5~5.0, after stirring, keep 50~55 DEG C enzymolysis 2H, be then brought rapidly up to
100 DEG C of progress quick inactivatings, 80 DEG C of refluxing extraction 1H. are cooled to after inactivating 1MIN
(4) extract filtered, removed slag, being concentrated and dry extract.
Preferably, described compound biological enzyme presses 20 by cellulase, amylase, pectase, protease：5：6：3 ratios
Prepare.
Preferably, solvent described in step 3 is the one or more in water, ethanol, methanol, acetone, petroleum ether, n-hexane
Mixture.
Preferably, the dog rose fruit powder end and the feed weight ratio of Extraction solvent are 1:3~1:8.
Preferably, the dosage of the compound biological enzyme is the 0.5%~1% of rose fruit powder.
Preferably, the concentrate drying processing of the step (4) extract is that the dog rose fruit that extraction obtains is carried
Liquid is taken to input liquid separation tank, for control evaporating temperature between 55-65 DEG C, pressure control is concentrated by evaporation extract solution in -0.1-0Mpa
To the 25-45% of extract solution total amount, continue to obtain dog rose berry extract after being dried under reduced pressure 8h.
The dog rose berry extract that the present invention is extracted using biology enzyme-circumfluence method, is catalyzed, efficiency by enzyme preparation
Height, mild condition, dog rose fruit the effect of property is remained in maximum efficiency.Extract essence is rich in vitamin C, can promote skin
The ability of skin hyperplasia neoblast, has that moistness is strong, the double grading of high osmosis, can maintain the elastic of all cells and moisten
Pool, thus be widely used in cosmetic field, thing of the present invention is applied in skin care cream.
A kind of efficiently moist preserving moisture and protecting skin cream, by weight percentage including following component：
0.1~30.0% NMF；0.1~8% emulsifying agent；0.1~30% emollient；0.1-10% dog teeth
Rose hip extract；0.1~2% stable thickener；0.01~3% additive；Preservative≤1.0%；And supplemented with water
To 100%.
Wherein, it is preferred that the NMF is selected from 1,3-BDO, propane diols, glycerine, glycine betaine, honey natural moisture preserving
One or more of mixtures in the factor.It is furthermore preferred that glycine betaine and 1,3-BDO, the honey for natural origin are naturally protected
One or more of mixtures in the wet factor, glycerine.Glycine betaine is to be extracted from beet root completely, pure containing 99% active matter
Gently, it is same tackle that oxidation is stimulated and UV is damaged except assigning moisturizing performance, in addition can also stimulation collagen conjunction
Into making skin flexible.
Preferably, the emulsifying agent be selected from tristerin (and) PEG-100 stearates, cetyl glucoside,
One or several kinds of mixtures in ceteareth -20, soybean lecithin, more preferably above 2 kinds in proportion
Collocation is used in mixed way.
Preferably, the emollient be hexadecanol, octadecyl alcolol, cetostearyl alcohol, dimethicone, isopropyl palmitate,
Jojoba oil, cera alba, lanolin, vaseline, one or several kinds of mixtures in shea butter.More preferably butter fruit
Fat and other greases are used in mixed way in proportion.
Preferably, the stable thickener is selected from acrylate copolymer, hydroxyethyl cellulose, xanthans, hydroxylethyl
One or more of mixtures in acid esters/sodium acryloyldimethyl taurate copolymers.
Preferably, the additive is selected from phytosterol, oil-soluble pot marigold extract, Marine fish collagen and Mo Luo
At least two in brother's macadamia nut oil.Phytosterol has very high permeability to skin, can keep skin surface moisture content, promotes skin
Skin metabolism, suppress scytitis, can anti-sunburn, skin aging, there is good skin sense (spreadability good, smooth do not glue)
Etc. characteristic.
Pot marigold grows as garden plant all over the world, is a kind of one of most useful medical draft.With nature
The effect of anti-corrosion and anti-inflammatory, it is to treat one of wound, optimal draft at skin abrasion.Making an addition in cosmetics to mitigate
Skin trauma, sunburn, and the cells grown of new skin can be stimulated.
Marine fish collagen is a kind of collagen of natural origin, and this albumen is found to be connection tissue and skin
In major protein (be more than 75%), it plays critically important effect in terms of the tone of skin, softness and elasticity is maintained, and has
There is extremely low molecular weight, must amino acid containing 8 kinds of human bodies for accounting for all total amino acid contents 14.5%.
Argan oil contains abundant fatty acid, sterol, vitamin e and phenolic compound.Including largely must
Need aliphatic acid, unrighted acid and linseed oil, these be all can not be synthesized in human body and must be got from fat
's.And polyphenol is well-known antioxidant, it can not only delay skin aging, can be with other anti-oxidant in prolonged human body
Agent, such as vitamin E, ascorbic action time.
Preservative used in the present invention is the preservative that the art is commonly used.
The present invention apply biology enzyme-backflow preparation method, by enzyme destruction cell membrane compact texture, cause cell membrane and
The generation of cytoplasm structure is local loose, expansion, reduces the active ingredient of cell membrane and cytoplasm barrier from intracellular to extraction
The resistance to mass tranfer of Medium Diffusion, so as to be advantageous to active ingredient dissolution.The method also has high catalytic efficiency, catalytic condition temperature
With, it can reduce efficiency, reduce pollution, the characteristics of simplifying process.The dog rose berry extract of the present invention is natural, peace
Entirely, it is added into skin care cream, moist moisture-keeping efficacy well can be played, moreover it is possible to improved skin barrier function, remain thin
Born of the same parents' is elastic and moist.
Brief description of the drawings
Fig. 1 is extraction process flow chart of the present invention.
Embodiment
With reference to embodiment, the present invention will be described in detail, and embodiment is only the preferred embodiment of the present invention,
It is not limitation of the invention.
Embodiment 1
A kind of preparation method of dog rose berry extract, is comprised the following steps：
(1) the dog rose fruit of drying is taken, ultramicro grinding can cross 100 mesh sieves.
(2) compound biological enzyme is prepared, cellulase, amylase, pectase, protease press 20：5：6：3 ratios are prepared, enzyme
Active unit be 500,000 U/g
(3) the dog rose fruit powder after crushing is added into deionized water, temperature is then risen to 55 DEG C, then added compound
Biology enzyme, it is 4.5 with acid for adjusting pH, after stirring, keeps 55 DEG C of enzymolysis 2H, be then brought rapidly up to 100 DEG C carrying out quickly
Inactivation, 80 DEG C of refluxing extraction 1H. are cooled to after inactivating 1MIN
(4) the cynarrhodion extract solution for filter extract, remove slag, extraction being obtained inputs liquid separation tank, control evaporation
For temperature between 65 DEG C, pressure control makes extract solution be concentrated by evaporation to the 30% of extract solution total amount in -0.1Mpa, and it is dry to continue decompression
Dog rose fruit is obtained after dry 8h to skim the cream off milk.
Embodiment 2
A kind of preparation method of dog rose berry extract, is comprised the following steps：
(1) the dog rose fruit of drying is taken, ultramicro grinding can cross 100 mesh sieves.
(2) compound biological enzyme is prepared, cellulase, amylase, pectase, protease press 6：3：2：2 ratios are prepared, enzyme
Active unit is 500,000 U/g
(3) the dog rose fruit powder after crushing is added into ethanol, temperature is then risen to 50 DEG C, then adds compound bio
Enzyme, it is 4.8 with acid for adjusting pH, after stirring, keeps 50 DEG C of enzymolysis 2H, is then brought rapidly up to 100 DEG C of progress quick inactivatings,
80 DEG C of refluxing extraction 1H. are cooled to after inactivation 1MIN
(4) the cynarrhodion extract solution for filter extract, remove slag, extraction being obtained inputs liquid separation tank, control evaporation
For temperature between 60 DEG C, pressure control makes extract solution be concentrated by evaporation to the 30% of extract solution total amount in -0.1Mpa, and it is dry to continue decompression
Dog rose fruit is obtained after dry 8h to skim the cream off milk.
Embodiment 3
A kind of preparation method of dog rose berry extract, is comprised the following steps：
(1) the dog rose fruit of drying is taken, ultramicro grinding can cross 100 mesh sieves.
(2) compound biological enzyme is prepared, cellulase, amylase, pectase, protease press 4：1：1：2 ratios are prepared, enzyme
Active unit is 500,000 U/g
(3) the dog rose fruit powder after crushing is added into methanol, temperature is then risen to 55 DEG C, then adds compound bio
Enzyme, it is 5.0 with acid for adjusting pH, after stirring, keeps 50 DEG C of enzymolysis 2H, is then brought rapidly up to 100 DEG C of progress quick inactivatings,
80 DEG C of refluxing extraction 1H. are cooled to after inactivation 1MIN
(4) the cynarrhodion extract solution for filter extract, remove slag, extraction being obtained inputs liquid separation tank, control evaporation
For temperature between 55 DEG C, pressure control makes extract solution be concentrated by evaporation to the 30% of extract solution total amount in 0Mpa, continues to be dried under reduced pressure
Dog rose fruit is obtained after 8h to skim the cream off milk.
Embodiment 4
A kind of moist moisturizing cream, include the component of following percentage by weight：
3% glycine betaine, 5% glycerine, 2% cetyl glucoside, 1.5% tristerin (and) PEG-100 tristearin
Acid esters, 2% shea butter, 3% isopropyl palmitate, 1.5% dimethicone, 1.5% jojoba oil, 0.2% acrylic acid gather
Compound, 0.1%EDTA-2Na, 0.5% phytosterol, 0.2% Marine fish collagen, 1% embodiment dog rose extract,
0.2% triethanolamine, 0.8% Phenoxyethanol, 0.02% iodo propinyl butyl carbamate, 100% is complemented to water.
Embodiment 5
A kind of moist moisturizing cream, include the component of following percentage by weight：
3% glycine betaine, 5% glycerine, 2% cetyl glucoside, 1.5% tristerin (and) PEG-100 tristearin
Acid esters, 2% shea butter, 3% isopropyl palmitate, 1.5% dimethicone, 1.5% jojoba oil, 0.2% acrylic acid gather
Compound, 0.1%EDTA-2Na, 0.5% phytosterol, 0.2% Marine fish collagen, 3% embodiment dog rose extract,
0.2% triethanolamine, 0.8% Phenoxyethanol, 0.02% iodo propinyl butyl carbamate, 100% is complemented to water.
Embodiment 6
A kind of moist moisturizing cream, include the component of following percentage by weight：
3% glycine betaine, 5% glycerine, 2% cetyl glucoside, 1.5% tristerin (and) PEG-100 tristearin
Acid esters, 2% shea butter, 3% isopropyl palmitate, 1.5% dimethicone, 1.5% jojoba oil, 0.2% acrylic acid gather
Compound, 0.1%EDTA-2Na, 0.5% phytosterol, 0.2% Marine fish collagen, 5% embodiment dog rose extract,
0.2% triethanolamine, 0.8% Phenoxyethanol, 0.02% iodo propinyl butyl carbamate, 100% is complemented to water.
Comparative example
A kind of moist moisturizing cream, include the component of following percentage by weight：
3% glycine betaine, 5% glycerine, 2% cetyl glucoside, 1.5% tristerin (and) PEG-100 tristearin
Acid esters, 2% shea butter, 3% isopropyl palmitate, 1.5% dimethicone, 1.5% jojoba oil, 0.2% acrylic acid gather
Compound, 0.1%EDTA-2Na, 0.5% phytosterol, 0.2% Marine fish collagen, 0.2% triethanolamine, 0.8% benzene
Oxyethanol, 0.02% iodo propinyl butyl carbamate, 100% is complemented to water.
The preparation method of the present invention is conventional oil-water emulsion hybrid technology, is not elaborated herein.
By contrast test, moisten moisturizing cream test process and the results are shown in Table one：
First look for the drier volunteer of skin totally 40 people, every one group of 5 people, totally 8 groups, respectively using embodiment and contrast
Example totally four products, continuous use 10 days, is further continued for tracking 5 days, by 10 skin experts daily before using product to it
Skin is evaluated, and every group takes its average value, and as a result such as following table one, (wherein 1-10 refers to skin health index, and index is higher, skin
Skin is more healthy)
The volunteer of table 1 is to moistening moisturizing cream and sample for reference effect assessment fraction
| Group | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 |
| Embodiment 4 | 6 | 7 | 8 | 8 | 6 | 8 | 8 | 6 |
| Embodiment 5 | 8 | 8 | 8.5 | 8.5 | 8 | 8.5 | 9 | 8 |
| Embodiment 6 | 10 | 9.5 | 8.5 | 9 | 9 | 9.5 | 9 | 9 |
| Comparative example | 5 | 4 | 5 | 5 | 2 | 4 | 5 | 5 |
Find out respectively have to skin using containing 1%, 3%, 5%, 0% dog rose extract from the experimental data of table one
Moist moisturizing and repair well, very little is acted on to skin repair without dog rose extract.Contain as can be seen from the table
The moist moisturizing of 5% dog rose extract and repair effect than it is common the effect of effect improve 2 times.
Claims (6)
- A kind of 1. efficiently moist preserving moisture and protecting skin cream, it is characterised in that：Include following component by weight percentage：The .0% of 0 .1~30 NMF；The emulsifying agent of 0 .1~8%；The emollient of 0 .1~30%；1-10% dog tooth roses Common vetch berry extract；The stable thickener of 0 .1~2%；The additive of 0 .01~3%；The .0% of preservative≤1；And mended with water It is charged to 100%；The preparation method of the dog rose berry extract, comprises the following steps：(1) the dog rose fruit of drying, Ultramicro-powder are taken It is broken to cross 100 mesh sieves；(2) compound biological enzyme is prepared, the active unit of enzyme is 500,000 U/g；(3) by the dog tooth after crushing Rose fruit powder is added in solvent, and temperature then is risen into 50~55 DEG C, then adds compound biological enzyme, is 4.5 with acid for adjusting pH ~5.0, after stirring, 50~55 DEG C of enzymolysis 2H are kept, is then brought rapidly up to 100 DEG C of progress quick inactivatings, inactivates 1MIN After be cooled to 80 DEG C of refluxing extraction 1H；(4) extract filtered, removed slag, being concentrated and dry extract；The NMF is the one or more in 1,3 butylene glycol, propane diols, glycerine, glycine betaine, honey NMF Mixture；The emulsifying agent be tristerin, PEG-100 stearates, cetyl glucoside, ceteareth- 20th, one or several kinds of mixtures in soybean lecithin；The emollient is hexadecanol, octadecyl alcolol, cetostearyl alcohol, dimethicone, isopropyl palmitate, jojoba oil, white Beeswax, lanolin, vaseline, one or several kinds of mixtures in shea butter；The stable thickener is selected from acrylate copolymer, hydroxyethyl cellulose, xanthans, hydroxy ethyl methacrylate/acryloyl One or more of mixtures in dimethyltaurine sodium copolymer；The additive is in phytosterol, oil-soluble pot marigold extract, Marine fish collagen and argan oil At least two.
- 2. efficiently moist preserving moisture and protecting skin cream according to claim 1, it is characterised in that described compound biological enzyme is by fibre Plain enzyme, amylase, pectase, protease are tieed up by 20：5：6：3 ratios are prepared.
- 3. efficiently moist preserving moisture and protecting skin cream according to claim 1, it is characterised in that solvent described in step 3 be water, One or several kinds of mixtures in ethanol, methanol.
- 4. efficiently moist preserving moisture and protecting skin cream according to claim 1, it is characterised in that the dog rose fruit powder end with The feed weight ratio of solvent is 1:3~1:8.
- 5. efficiently moist preserving moisture and protecting skin cream according to claim 1, it is characterised in that the dosage of the compound biological enzyme For the 0.5%~1% of dog rose fruit powder.
- 6. efficiently moist preserving moisture and protecting skin cream according to claim 1, it is characterised in that step (4) described extract It is that the dog rose fruit extract solution that extraction is obtained inputs liquid separation tank to be concentrated and dried processing, control evaporating temperature 55-65 DEG C it Between, pressure control makes extract solution be concentrated by evaporation to the 25-45% of extract solution total amount in -0.1-0Mpa, continues after being dried under reduced pressure 8h Obtain dog rose berry extract.
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| CN105997837A (en) * | 2016-06-17 | 2016-10-12 | 广州岚萃贸易有限公司 | Formula of spray for removing cutin |
| CN107375079A (en) * | 2017-08-04 | 2017-11-24 | 澳宝化妆品（惠州）有限公司 | A kind of extracting method of cacao seed extract and its application in hand frost |
| CN108379126A (en) * | 2018-04-26 | 2018-08-10 | 广东岭南职业技术学院 | A kind of fat of Oromaius norvaehollandeae cold cream and preparation method thereof |
| FR3090380B1 (en) * | 2018-12-20 | 2021-09-17 | Lvmh Rech | ROSEWOOD EXTRACT |
| CN111903978A (en) * | 2020-07-02 | 2020-11-10 | 太阳树（厦门）生物工程有限公司 | Production method of rose hip extract containing VC |
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