CN105455129B - Natural bionic process based on cold chain, new product produced by natural bionic process based on cold chain and application - Google Patents
Natural bionic process based on cold chain, new product produced by natural bionic process based on cold chain and application Download PDFInfo
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/06—Fungi, e.g. yeasts
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/19—Acanthaceae (Acanthus family)
- A61K36/195—Strobilanthes
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/25—Araliaceae (Ginseng family), e.g. ivy, aralia, schefflera or tetrapanax
- A61K36/258—Panax (ginseng)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/31—Brassicaceae or Cruciferae (Mustard family), e.g. broccoli, cabbage or kohlrabi
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/31—Brassicaceae or Cruciferae (Mustard family), e.g. broccoli, cabbage or kohlrabi
- A61K36/315—Isatis, e.g. Dyer's woad
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
- A61K36/488—Pueraria (kudzu)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/898—Orchidaceae (Orchid family)
- A61K36/8984—Dendrobium
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/899—Poaceae or Gramineae (Grass family), e.g. bamboo, corn or sugar cane
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/906—Zingiberaceae (Ginger family)
- A61K36/9068—Zingiber, e.g. garden ginger
Abstract
The invention discloses a natural bionic process based on a cold chain, a new product produced by the natural bionic process based on the cold chain and application. The natural bionic process comprises the steps of pretreatment, bionic extraction, separation and concentration, sterilization and drying. The application of the natural bionic technology based on the cold chain in the preparation of the biological original ecological activity essence. The invention relates to a biological essence concentrated solution, which is prepared by carrying out cold chain transportation, storage and fresh keeping on fresh organisms, breaking the walls of fresh products, quick-frozen products, freeze-dried products or dried products, or grinding the fresh products, the quick-frozen products, the freeze-dried products or the dried products into pulp at low temperature, then putting the pulp into a membrane bioreactor, extracting by taking water as a medium, and separating, removing impurities, dehydrating and concentrating an extracting solution. A closed circulating cold reflux system is formed by the membrane bioreactor, the dialysis separation device and the dehydration concentration device, and can simultaneously carry out extraction, separation and concentration. Sterilizing the concentrated solution, immediately freezing and temporarily storing, freeze-drying to obtain dried substance, and packaging into powder, tablet or capsule.
Description
Technical Field
The invention belongs to the field of food deep processing technology and products, further belongs to the field of bioactive essence and active traditional Chinese medicine preparation processing technology and products, and particularly relates to a natural bionic process based on a cold chain, a new product produced by the natural bionic process based on the cold chain and application of the new product.
Background
The biological nutrient components are divided into two types: one is biochemical active ingredient, such as polysaccharide, amino acid, ketone, phenol, etc.; the other is survivability effective components, such as vitamins, enzymes, hormones, and parahormones; and volatile oil component for showing plant fragrance. These components constitute the complete biologically effective active ingredient. For a long time, people only pay attention to the fact that biochemical active ingredients or a certain monomer are extracted by a traditional extraction method, and the functional activity generated by the active ingredients of plants does not take the effect of single ingredient but is the result of the unified coordination effect of a plurality of complex ingredients. For example, vitamin C should act with bioflavonoids to show antioxidant and antiaging effects.
Research shows that the biological polysaccharide needs to show functional activity, the original ecological space double-chain structure needs to be kept and not damaged, the integrity of the space structure needs to be ensured by using a low-temperature, low-pressure and mild method for extracting the biological active polysaccharide, and in addition, the pharmacodynamic activity of a plurality of active ingredients is greatly reduced due to the change of the space structure and other reasons after the high-temperature extraction.
The biochemical activity of the organism is relatively stable and does not lose activity under high temperature and high pressure. The extraction process using high temperature or high pressure ultrasonic wave, microwave, etc. can result in most of the protein denaturation and loss of activity in the extraction process. In addition, biological special aroma components are volatilized quickly once high temperature or boiling phenomenon is generated in the extraction process. It is highly desirable to develop a method for obtaining the intact active fraction, i.e., the original active essence, from the living organism without destroying its activity.
The digestive system of human body is specially used for taking the effective components of living things, and the gastrointestinal liver, gall bladder and kidney of human body form the digestive, absorptive and decomposing system of human body for food. The system has both physical and biochemical processes, and human body only uses water as solvent, and does not use industrial organic solvent to extract effective components. There are many people currently simulating the environment of the stomach to achieve semi-biomimetic extraction. The inventor carries out more than ten years of intensive research and development, from a small test to a middle test, and then to small-scale production; a primary fully bionic process for biological extraction combined with a raw cold chain process is developed, and bionic uptake, enrichment, concentration and drying of bioactive components under a low-temperature cold chain condition are realized.
Disclosure of Invention
The first purpose of the invention is to provide a natural bionic process based on a cold chain; the second purpose is to provide a new product produced by the natural bionic process based on the cold chain, namely, biological original ecological active essence, a fresh traditional Chinese medicine preparation or active traditional Chinese medicine instant decoction pieces; the third purpose is to provide the application of the natural bionic process based on the cold chain, and a low-temperature pre-extraction process for further extracting the bioactive effective component monomer in the pharmaceutical industry.
The first purpose of the invention is realized by the steps of pretreatment, bionic extraction, separation and concentration, sterilization and drying, and the method specifically comprises the following steps:
A. pretreatment: grinding fresh, frozen, freeze-dried or dried raw materials into pulp or breaking the wall, and breaking the wall of the freeze-dried or dried product;
B. bionic extraction: placing the pretreated fresh raw material into a membrane bioreactor, adding deionized water with the weight ratio of 1: 3-10 of the raw material, and performing biomimetic extraction for 60-180 min to obtain an extracting solution;
C. separation and concentration: the extract is dehydrated and concentrated after dialysis and separation to obtain concentrated solution;
D. and (3) sterilizing and drying: sterilizing the concentrated solution at low temperature, quickly freezing to below-20 ℃, and freeze-drying to obtain the original ecological active essence.
The second purpose of the invention is realized by the new product produced by the natural bionic process based on the cold chain, and the produced new product is biological original ecological active essence, a fresh traditional Chinese medicine preparation or active traditional Chinese medicine instant decoction pieces.
The third purpose of the invention is realized by the application of the natural bionic process based on the cold chain in the low-temperature pre-extraction process for further extracting the bioactive effective component monomer.
The invention relates to a biological essence concentrated solution, which is prepared by the steps of carrying out cold chain transportation, storage and fresh keeping on fresh organisms, grinding, powdering and breaking walls on fresh products, quick-frozen products, FD freeze-dried products or dry products, then putting the fresh products, the quick-frozen products, the FD freeze-dried products or the dry products into a membrane bioreactor, extracting by taking water as a medium, and separating, removing impurities and dehydrating an extracting solution. A closed circulating cold reflux system is formed by the membrane bioreactor, the dialysis separation device and the dehydration concentration device, and can simultaneously carry out extraction, separation and concentration. And sterilizing the concentrated solution, quickly freezing, freeze-drying to obtain a dried substance, and packaging or tabletting the dried substance to obtain the original ecological active essence preparation which is convenient to store and carry at normal temperature.
The process of the invention has the technical characteristics that:
(I) raw Material
The raw materials are preferably fresh products of cold chain logistics, instant frozen products to ensure no loss of active ingredients from the source, and FD freeze-dried products or dry products can be adopted as the factors for convenient storage of the raw materials.
(II) Cold chain Process
The whole process is a cold chain process, fresh plants are transported and stored from the cold chain, the temperature of the whole process section does not exceed the normal temperature or is preferably lower than 25 ℃, and concentrated essence is immediately quick-frozen and then temporarily stored; the ice cake of the concentrated solution is frozen and dried after being crushed, and the sublimation temperature of the frozen and dried ice cake is lower than-18 ℃.
(III) Natural bionic technology
1. The grinding process simulates the function of oral teeth.
2. The membrane bioreactor comprises a tank type membrane bioreactor and a channel type membrane bioreactor, which respectively simulate the structural morphology and the digestion mode of the stomach and the intestinal tract of a human. Gastric and intestinal motility were simulated with a low pressure pump, a stirrer and a peristaltic pump.
3. The steps of separating, removing impurities, removing heavy metals, dehydrating and concentrating adopt a dialysis method to simulate the absorption function and the renal function of human gastrointestinal mucosa.
4. The material residue dehydration adopts progressive spiral extrusion type dehydration to simulate the function of the large intestine.
5. The bionic process can be divided into primary physical bionics and primary physical biochemical bionics, and the physical bionics is then not added any chemical composition except water, and the temperature is controlled as far as possible and is as low as possible, in order to avoid plant self enzymolysis reaction: the physical and biochemical bionics are realized by adding enzyme or other components required by gastrointestinal digestion into a reactor.
Fourthly, the product produced by the natural bionic process based on the cold chain is concentrated enrichment essence based on the preservation of the complete activity of the original ecology of the organism, and is characterized in that the aroma components of the original ecology are also preserved; is in the form of a preparation which is convenient to store, carry and take at normal temperature; in the food industry, the raw active essence (raw active essence); is called as a fresh traditional Chinese medicine preparation or an active traditional Chinese medicine instant decoction piece in the traditional Chinese medicine industry.
The invention has the advantages that:
1. the bionic process is firstly combined with cold chain conditions, and the activity retention of the biological effective components in the process of ingestion is realized.
2. The primary full bionic technology is initiated, namely the stomach function is simulated, and the intestinal tract and kidney functions are simulated at the same time.
3. The product produced by the innovative process keeps the original ecological complete active ingredients, namely the survivability and biochemical active ingredients and the original ecological aromatic ingredients, thereby creating a new product which is biologically extracted, namely original ecological active essence, a fresh traditional Chinese medicine preparation or active traditional Chinese medicine instant decoction pieces.
Detailed Description
The present invention is further illustrated by the following examples, which are not intended to be limiting in any way, and any modifications or alterations based on the teachings of the present invention are intended to fall within the scope of the present invention.
The natural bionic process based on the cold chain comprises the steps of pretreatment, bionic extraction, separation and concentration, sterilization and drying, and specifically comprises the following steps:
A. pretreatment: grinding fresh, frozen, freeze-dried or dried raw materials into pulp or breaking the wall, and breaking the wall of the freeze-dried or dried product;
B. bionic extraction: placing the pretreated fresh raw material into a membrane bioreactor, adding deionized water with the weight ratio of 1: 3-10 of the raw material, and performing biomimetic extraction for 60-180 min to obtain an extracting solution;
C. separation and concentration: the extract is dehydrated and concentrated after dialysis and separation to obtain concentrated solution;
D. and (3) sterilizing and drying: sterilizing the concentrated solution at low temperature, quickly freezing to below-20 ℃, and freeze-drying to obtain the original ecological active essence.
The wall breaking rate of the wall breaking in the step A is more than 90%.
And the membrane bioreactor in the step B comprises a tank type membrane bioreactor and a channel type membrane bioreactor so as to simulate the structural morphology and the digestion mode of the stomach and the intestinal tract.
The bionic extraction temperature in the step B is that the physical bionic temperature is below 35 ℃, and the physical biochemical bionic temperature is below 45 ℃.
A. The process temperature in step B, C is controlled to be below 45 ℃.
The sublimation temperature in the freeze-drying described in step D is < -18 ℃.
The product produced by the natural bionic process based on the cold chain is original ecological active essence, a fresh traditional Chinese medicine preparation or active traditional Chinese medicine instant decoction pieces; the application is the application in the low-temperature pre-extraction for further extracting effective active monomers in the pharmaceutical industry.
The following specific embodiments further illustrate the invention:
example 1
Grinding 100kg of fresh tricholoma matsutake into pulp at low temperature, feeding into a membrane bioreactor, adding 300kg of deionized water, and starting physical bionic extraction at the temperature of below 15 ℃ for 75 minutes at the extraction temperature of less than 15 ℃; the extract is dehydrated and concentrated after dialysis separation to obtain 10kg of concentrated solution; the concentrated solution is quickly frozen to-40 ℃ after being sterilized at low temperature and ultrahigh pressure. Freeze drying to obtain 1.4kg of original tricholoma matsutake essence, and packaging into 1 g of original tricholoma matsutake active essence powder without any additive through nitrogen sealing.
The volatile oil content of the tricholoma matsutake extract and the relative content of the tricholoma matsutake characteristic aroma components 1-octene-3-alcohol and methyl cinnamate in the total volatile oil are measured by adopting a gas chromatography-mass spectrometry (GC-MS) linkage method, which comprises the following steps:
note: the traditional process of extracting the tricholoma matsutake refers to dry powder obtained by extracting the tricholoma matsutake with hot reflux water, drying and concentrating under vacuum and reduced pressure and drying under vacuum.
Therefore, compared with the traditional process, the tricholoma matsutake original ecological essence extracted by the process disclosed by the invention retains the essence of aroma characteristic components, and the aroma component extraction of the plants is the most difficult part to extract and retain in the plant extraction, particularly in a water extraction method, so that the complete extraction and enrichment of the original ecological aroma components in the extract can indicate that other active components are also completely extracted and enriched.
Example 2
30kg of fresh moringa leaves are used, ground into thick liquid and then fed into a membrane bioreactor. Adding 90kg of deionized water to start physical bionic extraction, wherein the time is 65 minutes and the temperature is lower than 20 ℃. And (3) carrying out dialysis separation and centrifugation on the extract, dehydrating and concentrating to obtain 9kg of concentrated solution, carrying out low-temperature sterilization on the concentrated solution, then immediately quickly freezing to the temperature below-40 ℃, then carrying out freeze drying to obtain 1.8kg of freeze-dried powder, and carrying out freeze drying and powder tabletting to obtain the moringa oleifera original ecological active essence tablet.
The analysis was carried out by GC-MS gas chromatography with HP-7890A-5975C coupled with mass spectrometry.
The content of volatile oil in the sample of the original moringa oleifera essence prepared in the embodiment 2 is more than or equal to 0.06%, and the content of volatile oil in the fresh leaves of the raw materials is more than or equal to 0.02%.
The original moringa oleifera essence can well retain the chlorophyll of the moringa oleifera by controlling the separation precision in the bionic extraction process, so that the extract is dark green.
Example 3
Washing 10kg of fresh maca, adding 10kg of deionized water at the low temperature of below 20 ℃, pulping by using a pulping machine, then feeding into a membrane bioreactor, and adding 40kg of deionized water to perform primary physical biochemical bionic extraction. Adjusting pH to 6.5 with acetic acid, adding 0.2 g of two kinds of bifidobacterium lyophilized powder respectively, and starting to react for 135 minutes at 38 ℃. Then clarifying, separating to remove impurities, concentrating and dehydrating to obtain 7.5kg of concentrated solution. The concentrated solution was sterilized at low temperature and ultra high pressure, immediately frozen to below-40 deg.C, and then freeze-dried to obtain 800g of dry powder. And then the maca original ecological active essence powder is prepared after processing and packaging.
And (3) carrying out GC-MS (gas chromatography-mass spectrometry) analysis and detection by using HP-7890A-5975C.
The proportion of the content of the original maca ecological active essence benzyl cyanide and benzyl isothiocyanate in the total amount of the glucosinolate decomposed volatile oil prepared in the embodiment 3 is as follows:
note: the traditional technology of the maca extract is the maca extract obtained by extracting maca with hot reflux water, then carrying out vacuum reduced pressure evaporation and concentration, and drying with a vacuum microwave oven.
It can be seen that in the traditional extraction process, the natural forward decomposition of maca glucosinolate is prevented technically, so that the decomposition products are mostly harmful phenylacetonitrile and a small part of benzyl isothiocyanate. In example 3, the glucosinolates of maca are naturally decomposed by using self myrosinase under bionic conditions by using a natural bionic process, so that most of benzyl isothiocyanate serving as an effective component can be generated, and only a small part of benzyl cyanide serving as a harmful component is generated.
Example 4
30kg of fresh bitter bamboo is used, and is subjected to fiber breaking, crushing and pulp grinding, and then enters a membrane bioreactor for physical bionic extraction, 300kg of water is added, the extraction time is 65 minutes, and the extraction temperature is less than 20 ℃. Clarifying the extractive solution, concentrating, dehydrating to obtain 18kg concentrated solution, sterilizing at low temperature and ultrahigh pressure, quickly freezing to below-40 deg.C, freeze drying to obtain lyophilized powder, packaging to obtain active fresh succus Bambusae essence, and further processing and packaging to obtain fresh succus Bambusae medicinal powder.
The fresh bamboo juice powder is used for brewing a cup of water, so that the bamboo can smell the faint scent immediately, the liquor color is yellow green, and the taste is sweet and slightly bitter. The fresh bamboo juice sold in the pharmacy in the market is coffee-colored, has no fragrance and only has the taste of ointment.
The traditional Chinese medicine holds that the fresh bamboo juice should be taken orally as a fresh medicine, and has obvious effects of clearing lung and clearing blood vessels. However, due to the problem of the preservation technology, the fresh bamboo juice sold on the market at present is not a fresh medicine, but is an aqueous solution prepared by boiling at high temperature, so that the medicinal activity is greatly reduced.
The fresh bamboo juice fresh medicine powder produced by the process of the invention completely retains the active ingredients of the fresh bamboo juice under the condition of cold chain, and solves the problems of storage, carrying and taking methods.
Example 5
The method comprises the steps of cleaning, slicing and grinding 30kg of fresh radix puerariae, separating to remove long fibers, adding 120kg of deionized water, and entering a membrane bioreactor and a dialysis separation and concentration device to form circulating cold reflux extraction, separation and concentration. In order to prevent the effective components of the kudzuvine root from being oxidized, the storage tank machine container of the whole process engineering is subjected to nitrogen sealing to isolate oxygen. Sterilizing the concentrated solution at low temperature, immediately freezing to below-40 deg.C, and freeze drying to obtain 0.61kg radix Puerariae active essence lyophilized powder. Further processing and packaging to prepare the kudzu root fresh medicine powder.
The volatile oil component of the fresh radix puerariae preparation is 1.6 percent and the volatile oil component of the fresh radix puerariae is 0.1 percent by using HP-7890A-5975C GC-MS gas chromatography and mass spectrometry combined analysis and detection, which shows that the volatile oil component of the fresh radix puerariae preparation produced by the process of the invention is 16 times enriched on the basis of the enrichment of the effective components. The volatile oil content of the commercially available radix puerariae powder traditional Chinese medicine decoction pieces and radix puerariae extracts is not detected by the same method.
The taste of the radix puerariae essence powder after being infused with water is basically the same as the fragrance and the taste of the freshly squeezed radix puerariae juice, and the commercially sold radix puerariae powder decoction pieces and radix puerariae extract substitutional tea basically have no taste and fragrance of radix puerariae.
Example 6
10kg of artificially cultured African cockroach is adopted, 30kg of water is added after cleaning, pulping and crushing are carried out, 30kg of deionized water is added, and the mixture enters a membrane bioreactor for physical bionic extraction, wherein the extraction temperature is less than 12 ℃, and the extraction time is 60 minutes. The extract is dewatered and concentrated after being clarified to obtain 7kg of concentrated solution, the concentrated solution is quickly frozen after being sterilized at low temperature, and is frozen and dried to obtain 780 g of freeze-dried powder, and the 780 g of freeze-dried powder is encapsulated according to 0.5 g/capsule to prepare a capsule fresh medicine preparation.
The control sample is a periplaneta americana water-soluble extract, the raw materials are dried by the traditional method, crushed, leached, subjected to impurity separation, evaporated and concentrated in vacuum, and then dried under reduced pressure at low temperature to obtain the periplaneta americana traditional process extract control sample.
Periplaneta americana extract activity experimental analysis
Experimental mice: 90 Kunming mice with the weight of 18g +/-2 g are purchased from the center of experimental animals of Kunming medical college.
The experimental method comprises the following steps: dissolving the periplaneta Americana active extract and the extract of a traditional process control sample in deionized water, and performing intragastric administration according to the weight of 120mg/kg, wherein 30 intragastric active extracts are used in a group I, 30 intragastric traditional control aqueous extracts are used in a group II, and 30 intragastric physiological saline is used as a blank sample and is 1 ml. Blood was drawn 7 days after continuous gavage to test for white blood cell counts.
| Item | Blank space | Group I | Group II |
| White blood cell count (10)9/L ） | 4.51 | 5.28 | 4.74 |
From the statistics of the white blood cell count, the white blood cell count activity extract is improved by 17.1 percent, compared with the extract obtained by the traditional process, the white blood cell count activity extract is improved by 5.1 percent, and the improvement rate of the white blood cell count activity extract is more than 3 times that of the extract obtained by the traditional process.
Example 7
Taking 10kg of crushed black tea, further crushing, adding 90kg of deionized water, entering a membrane bioreactor for biomimetic extraction, wherein the extraction temperature is less than 45 ℃, and the extraction time is 85 minutes. Separating and concentrating the extracting solution to obtain 38kg of concentrated solution, freeze-drying the concentrated solution to obtain 2.1kg of dry powder, and packaging the dry powder into 0.5 g of strip bags to prepare the original-taste instant black tea.
The volatile oil component of the original tea aroma is detected to be 9mg/100g by using HP-7890A-5975C GC-MS gas and mass spectrum combined analysis, and the aroma component of the extract instant black tea is detected to be 33mg/100 g. The aroma components of the black tea are well preserved and enriched, and the aroma components of the traditional commercially available instant black tea are not detected to be 3mg/100 g.
Example 8
10kg of fresh pseudo-ginseng with the same production area and the same size is prepared into a freeze-dried product, and 10kg is prepared into a low-temperature dried product. Freeze-drying 10kg of pseudo-ginseng, crushing to 100 meshes, adding 100kg of deionized water, entering a membrane bioreactor for physical bionic extraction at the extraction temperature of less than 20 ℃ for 90 minutes. Extracting, concentrating, separating and concentrating to obtain 12kg of extract concentrate, performing pre-extraction, and performing chromatographic separation to obtain 855g of active panax notoginseng saponins with purity of more than 90%.
Taking 10kg of dried panax notoginseng, crushing the panax notoginseng into particles, extracting the particles by using ethanol, filtering an extracting solution by 0.2um, separating and purifying the extracting solution by using a chromatographic column, and evaporating and drying the extracting solution to obtain 610g of panax notoginseng saponins with the purity of more than 87 percent.
Using Shimadzu LC-10 ATVPExtracting Notoginseng radix effective components from dried product by high performance liquid chromatography (R g)1+Rb1+R1) The total content is aimed atThe control results of the mass percentages of the raw materials are as follows:
| same batch of samples | Extraction process | （R g 1+ Rb1+R1) % yield of active ingredient of extract |
| Fresh pseudo-ginseng | Cold chain natural bionic process | 6.96% |
| Dried pseudo-ginseng | Extracting with ethanol, evaporating, and drying | 5.32% |
Therefore, compared with the traditional extraction process of dried pseudo-ginseng, the new extraction process of the freeze-dried pseudo-ginseng as the raw material improves the yield of the effective components by 30.8 percent and has obvious improvement significance.
Example 9
10kg of dried isatis root is crushed to 80 meshes, 100kg of deionized water is added and enters a membrane bioreactor for physical bionic extraction, the extraction temperature is lower than 35 ℃, and the extraction time is 85 minutes. The extract is separated and concentrated, and freeze-dried by a freeze-drying machine to obtain dry paste. The contrast method is to take 10kg of radix isatidis of the same batch to be boiled with water and extracted by alcohol precipitation, and then dry paste is obtained by evaporation, concentration and drying. The isatis root extract prepared by the process can be used as an active instant traditional Chinese medicine decoction piece of isatis root.
The content of indirubin in dry paste is detected by a high performance liquid chromatograph
| Process for the preparation of a coating | Indirubin content (ug/g) |
| Natural bionic cold chain | 9.86 |
| Water boiling alcohol precipitation method | 0.84 |
Therefore, the process of the invention has the advantage that the retention of the heat-sensitive effective component indirubin is qualitatively improved compared with the traditional process.
Example 10
Taking 2kg of fresh dendrobium, cleaning, crushing, grinding into slurry, adding 20kg of deionized water, entering a membrane bioreactor for physical bionic extraction at the extraction temperature of less than 25 ℃, and freeze-drying after separating and concentrating the extracting solution to obtain 75g of primary extract freeze-dried powder. The lyophilized powder can be dissolved in water, and can be used as instant granule of herba Dendrobii.
And drying the control sample in the same batch of dendrobe drying oven, extracting 1kg of the dried sample with water, filtering the water extract with 1um, evaporating and drying to obtain 95g of dried extract, which is also the dendrobe extract decoction piece granule. The contents of dendrobium polysaccharide and alkaloid in the two extracts are tested, and compared as follows:
| item | Dendrobe polysaccharide (%) | Alkaloid (%) |
| Instant medicinal instant dendrobium stem granule | 39.6 | 0.065 |
| Instant medicinal instant granule of fresh dendrobium | 28.7 | 0.048 |
Therefore, the content of effective active ingredients of the fresh dendrobium instant decoction pieces extracted by the process is obviously improved compared with the content of the effective active ingredients of the fresh dendrobium instant decoction pieces extracted by the traditional process.
Example 11
Grinding 100kg of wild fresh bolete albus (delicious bolete) at low temperature, feeding the ground bolete albus into a membrane bioreactor, adding 300kg of deionized water, starting physical bionic extraction at the temperature of below 15 ℃ for 75 minutes, carrying out dialysis separation and centrifugation on an extracting solution, then dehydrating and concentrating to obtain 10kg of concentrated solution, carrying out low-temperature electric pulse sterilization on the concentrated solution, and immediately freezing to-40 ℃ for temporary storage; and crushing the ice blocks, and then sending the crushed ice blocks into a freeze drying bin for freeze drying to obtain 1.28kg of original delicious bovine liver essence, and packaging the original delicious bovine liver essence into 1 g/bag through nitrogen sealing to obtain original ecological delicious bovine liver essence powder without any additive.
And (3) extracting the control sample by using the same batch of washed slices and dried, heating and leaching with deionized water, filtering the extracting solution by 0.2um, performing reduced pressure evaporation and concentration to obtain an extract, and performing vacuum microwave drying on the extract to obtain the delicious traditional bovine liver water extract.
Experimental animals: 120 Kunming mice, weighing 18g +/-2 g, were purchased from Kunming medical college laboratory animal center.
Animals were divided into three groups: 40 of each group were subjected to low-dose irradiation for three consecutive days, and the test was started, KB group was blank group, group I was natural biomimetic extract group of the process of the present invention, and group II was water extract group of the conventional process. And (3) performing intragastric administration at the ratio of 80mg/kg, performing intragastric administration on a control group with 1mL of physiological saline, collecting 20uL of blood from tail veins of mice 7, 14 and 21d after the start of the experiment, placing the blood into a dry diluent, counting the number of blood cells by using a blood cell counting instrument, and taking an average value of each group according to counting results.
And (4) conclusion: therefore, the natural bionic process active extract has larger influence on the recovery and promotion of the immune mechanism of the mouse than the traditional process extract, and is obviously superior to the traditional extract.
Example 12
30kg of fresh ginger is ground into pulp at low temperature, 200kg of deionized water is added into the pulp and then the pulp is put into a membrane bioreactor for extraction, separation and concentration, and the temperature is lower than 35 ℃. And finally, quickly freezing and freeze-drying the concentrated solution to obtain 600g of freeze-dried powder, and packaging the freeze-dried powder in a drying and purifying room at the temperature of lower than 40 ℃ in a nitrogen seal manner to obtain 2 g/bag of fresh ginger active essence powder. Can be used as flavoring agent or health product.
The fresh ginger active essence has a more pungent taste than the decocted ginger soup, and has a much stronger pungent taste, and the content of gingerol is several times of that of the decocted ginger soup.
The anti-inflammatory, antibacterial, dehumidifying and cold-dispelling effects are greatly improved.
Claims (4)
1. A natural bionic process based on a cold chain is characterized by comprising the steps of pretreatment, bionic extraction, separation and concentration, sterilization and drying, and specifically comprises the following steps:
A. pretreatment: grinding fresh, frozen or dried raw materials into pulp or breaking the wall and crushing;
B. bionic extraction: putting the pretreated raw materials into a membrane bioreactor, adding deionized water with the weight ratio of 1: 3-10 of the raw materials, and performing physical biomimetic extraction for 60-180 min to obtain an extracting solution, wherein the process temperature is controlled at 35 ℃; the membrane bioreactor is a tank type membrane bioreactor or a channel type membrane bioreactor to simulate the structural morphology and the digestion mode of the stomach and the intestinal tract;
C. separation and concentration: the extract is dehydrated and concentrated after dialysis separation to obtain concentrated solution, the dehydration adopts progressive spiral extrusion type dehydration, and the process temperature is controlled at 45 ℃;
D. and (3) sterilizing and drying: sterilizing the concentrated solution at low temperature, quickly freezing to-20 deg.C, and freeze drying at sublimation temperature lower than-18 deg.C to obtain original ecological active essence.
2. The natural biomimetic process based on cold chain as in claim 1, wherein the wall breaking rate of the wall breaking in step a is above 90%.
3. The natural biomimetic process based on the cold chain as claimed in claim 1, wherein the mobile raw material after dialysis and separation and the water after concentration and dehydration in step C are refluxed to a membrane bioreactor reaction tank to realize cold reflux cycle extraction.
4. The use of the cold chain-based natural biomimetic process as claimed in any of claims 1-3 in a low temperature pre-extraction process for further extracting bioactive active ingredient monomers.
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| CN103789361A (en) * | 2014-02-20 | 2014-05-14 | 湖南化工职业技术学院 | Citric acid extraction method and membrane bioreactor for producing citric acid |
| CN104630316A (en) * | 2013-11-12 | 2015-05-20 | 中粮营养健康研究院有限公司 | Integrated technology for extracting proteins and phytin from corn steep liquor |
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| CN104630316A (en) * | 2013-11-12 | 2015-05-20 | 中粮营养健康研究院有限公司 | Integrated technology for extracting proteins and phytin from corn steep liquor |
| CN103789361A (en) * | 2014-02-20 | 2014-05-14 | 湖南化工职业技术学院 | Citric acid extraction method and membrane bioreactor for producing citric acid |
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