CN105455129A - Cold chain based natural bionic technology, novel product produced with cold chain based natural bionic technology as well as application - Google Patents

Cold chain based natural bionic technology, novel product produced with cold chain based natural bionic technology as well as application Download PDF

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CN105455129A
CN105455129A CN201510879220.0A CN201510879220A CN105455129A CN 105455129 A CN105455129 A CN 105455129A CN 201510879220 A CN201510879220 A CN 201510879220A CN 105455129 A CN105455129 A CN 105455129A
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cold chain
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extraction
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CN105455129B (en
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戴群
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Yunnan Huocaotang Biotechnology Co Ltd
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Yunnan Huocaotang Biotechnology Co Ltd
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    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/06—Fungi, e.g. yeasts
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/19—Acanthaceae (Acanthus family)
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    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/25—Araliaceae (Ginseng family), e.g. ivy, aralia, schefflera or tetrapanax
    • A61K36/258—Panax (ginseng)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/31—Brassicaceae or Cruciferae (Mustard family), e.g. broccoli, cabbage or kohlrabi
    • A—HUMAN NECESSITIES
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    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/31—Brassicaceae or Cruciferae (Mustard family), e.g. broccoli, cabbage or kohlrabi
    • A61K36/315—Isatis, e.g. Dyer's woad
    • A—HUMAN NECESSITIES
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    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
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    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/88—Liliopsida (monocotyledons)
    • A61K36/899—Poaceae or Gramineae (Grass family), e.g. bamboo, corn or sugar cane
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/88—Liliopsida (monocotyledons)
    • A61K36/906—Zingiberaceae (Ginger family)
    • A61K36/9068—Zingiber, e.g. garden ginger

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Abstract

The invention discloses a cold chain based natural bionic technology, a novel product produced with the cold chain based natural bionic technology as well as an application of the cold chain based natural bionic technology to preparation of biological original active essence. The natural bionic technology comprises steps of pretreatment, bionic extraction, separation and concentration as well as sterilization and drying. Fresh organisms are transported on a cold chain, stored and preserved, fresh products, quick-frozen products, freeze-dried products or dry products are subjected to wall breakage or low-temperature grinding firstly, then enter a membrane bioreactor and extracted with water used as a medium, an extraction solution is subjected to separation, impurity removal and dehydrating concentration, and a biological essence concentrate is obtained; the membrane bioreactor, a dialysis separation device and a dehydrating concentration device constitute a closed circulating cold reflux system, and extraction, separation and concentration can be performed simultaneously; the concentrate is immediately quick-frozen for temporary storage after being sterilized, then a dried substance, namely, the original active essence, is obtained after freeze-drying, and the original active essence is packaged in preparation forms of powder, tablets, capsules and the like.

Description

The new product of a kind of natural bionic technique based on cold chain and the natural bionic explained hereafter based on cold chain and application
Technical field
The invention belongs to food deep process technology and product scope, belong to biologically active elite and active Chinese medicine preparation process technology and product scope further, be specifically related to new product and the application of a kind of natural bionic technique based on cold chain and the natural bionic explained hereafter based on cold chain.
Background technology
Biological nutrition has two classes: a class is biochemical effective ingredient, as outside the compositions such as polysaccharide, amino acid, ketone, phenols; Another kind of is survivability effective ingredient, as vitamins, ferment class (enzyme), trophic hormone and parahormone isoreactivity composition; Be exactly the Constituents of The Essential Oil embodying scent in addition.These compositions constitute complete biologically effective active ingredient.For a long time, people only focus on extracting biochemical effective ingredient by traditional extracting method, or a certain monomer, the functional activity that the effective ingredient of plant produces not is that single composition plays active function, but the result of the unified coordinative role of Various Complex composition.As vitamin C will with biological flavone effect, just can show anti-oxidant, antidotal function.
There are some researches show that biological polyoses will show functional activity, the Space Double link structure that must retain ecosystem is not destroyed, extract bioactive polysaccharide and must use low temperature, low pressure, gentle method, ensure the integrality of its space structure, also have many active ingredients in addition after high temperature extracts because the reason drug activities such as space structure change reduce greatly.
Biological biochemical activity is more stable, unlikely devitalized at high temperature under high pressure.By extraction processes such as high temperature or high pressure ultrasonic wave, microwaves, can cause extracting most protein denaturation in engineering and losing activity.Biological special fragrance ingredient in addition, once produce high temperature or boiling phenomenon in leaching process, then can volatilize very soon.Develop a kind of can by the full activity part in biology, namely the active elite of ecosystem is absorbed out, and don't the method destroying its activity is very important.
The digestive system of the human body effective ingredient that picked-up is biological specially, the stomach and intestine liver and gall kidney of human body constitutes human body to the digestion of food, absorption, decomposing system.The existing physical process of this system, has biochemical process again, and human body only uses water as solvent, need not extract effective ingredient by industrial organic solvent.There is the environment of many human simulation stomaches at present to realize semi-bionic extraction method.And inventor is through the research and development with great concentration in more than ten years, from lab scale to pilot scale, then to small-scale production; Have developed the biology that a kind of and raw and cold chain process is combined and extract elementary full bionic artistic, realize the bionical picked-up of the biological activity under low temperature cold chain condition, enrichment, concentrated and dry.
Summary of the invention
The first object of the present invention is to provide a kind of natural bionic technique based on cold chain; Second object is to provide the new product of the described natural bionic explained hereafter based on cold chain, i.e. biological ecosystem active elite, Chinese medicine fresh medicine preparation or active Chinese medicine instant decoction pieces; 3rd object is to provide the application of the described natural bionic technique based on cold chain, for pharmaceutical industry extracts the low temperature preextraction technique of biologically active active ingredient monomer further.
The first object of the present invention is achieved in that and comprises that pre-treatment, bionic extraction, separation are concentrated, sterilizing-drying step, specifically comprises:
A, pre-treatment: raw material fresh goods, quick frozen product, dried frozen aquatic products or dry product are pulverized through defibrination or broken wall, dried frozen aquatic products or dry product sheet carry out beating powder broken wall;
B, bionic extraction: insert in membrane bioreactor by the raw material fresh goods after pre-treatment, add the deionized water of raw material weight than 1:3 ~ 10, carries out bionic extraction 60 ~ 180min and obtain extract;
C, separation concentrate: extract dehydration after dialysis is separated concentrates and obtains concentrate;
D, sterilizing-drying: by concentrate quick-frozen extremely less than-20 DEG C after low temperature sterilization, then obtain the active elite of ecosystem through freeze drying.
The second object of the present invention is achieved in that the new product of the described natural bionic explained hereafter based on cold chain, and the new product of described production is biological ecosystem active elite, Chinese medicine fresh medicine preparation or active Chinese medicine instant decoction pieces.
The third object of the present invention is achieved in that the described natural bionic technique based on cold chain is extracting the application in the low temperature preextraction technique of biologically active active ingredient monomer further.
In the present invention, fresh bio is from cold chain transportation, and storage fresh-keeping starts, and first carries out defibrination with fresh goods, quick frozen product or FD dried frozen aquatic products or dry product, beats powder and broken wall, then entering membrane bioreactor, is that medium extracts with water, and extract is through being separated, removal of impurities and dehydration, obtain biological elite concentrate.By membrane bioreactor, dialysis separator and dehydration enrichment facility form a closed circulation cold reflux system, can carry out extracting and developing simultaneously and concentrate.Concentrate is quick-frozen immediately after sterilization, then obtains dry thing after freeze drying, dry thing is packed again or compressing tablet namely become normal temperature under the active elite preparation of the ecosystem of conveniently preserving and carrying.
The technical characterstic of present invention process:
(1) raw material
The fresh goods of the most handy Cold Chain Logistics of raw material, quick frozen product, to ensure not losing of active ingredient from source, also can be storage factor easily, adopts FD freeze drying product or dry product.
(2) cold chain process
Whole process is a cold chain process, and fresh plant, from the transport of cold chain thing, storage, is no more than normal temperature in whole process section temperature or is preferably lower than less than 25 DEG C, temporarily store after the quick-frozen immediately of concentrated elite; Concentrate ice cube carries out freeze drying after trash ice, cryodesiccated sublimation temperature <-18 DEG C.
(3) natural bionic technique
1, the function of refining process Mouthsimulator tooth.
2, membrane bioreactor is containing pot type and channel-type membrane bioreactor, simulates the stomach of people and the structural form of enteron aisle and digestion pattern respectively.With low-lift pump, agitator and peristaltic pump simulation WeiDongLi Capsule and intestinal motive force.
3, separation, impurity removal and removing heavy metals and dehydration concentration step adopt dialysis process, the gastrointestinal mucosa absorption function of simulation people and renal function.
4, expect that slag dehydration adopts the dehydration of progressive helical squash type, simulate large gut function.
5, bionic process can be divided into that primary physical is bionical and primary physical is biochemical bionical, physics is bionical, does not add any chemical analysis than water, and temperature control to as far as possible more low better, to avoid plant self enzyme digestion reaction: physics is biochemical bionical, need the composition adding enzyme or other gastro-intestinal digestions needs in the reactor, realize biochemical bionical.
(4) by the enrichment method elite that the product that the natural bionic explained hereafter based on cold chain goes out is on the biological ecosystem full activity basis of reservation, its feature also retains the fragrance component of ecosystem; It is the dosage form conveniently stored, carry and take under normal temperature; The active elite (former cytokines) of ecosystem is called in food service industry; Chinese medicine fresh medicine preparation or active Chinese medicine instant decoction pieces is called at Chinese herbal medicine industry.
Advantage of the present invention:
1, pioneering bionic artistic to be combined with cold chain condition, realize the activity reservation of biologically effective composition in capture process.
2, pioneering elementary full bionic artistic, namely the function of not only bionical stomach, also simulates the function of enteron aisle and kidney simultaneously.
3, the product that innovative technology is produced remains the full activity composition of ecosystem, i.e. survivability and biochemical active ingredient, and the fragrance ingredient of ecosystem, thus started a kind of new product of biological extraction---ecosystem active elite, Chinese medicine fresh medicine preparation or active Chinese medicine instant decoction pieces.
Detailed description of the invention
Below in conjunction with embodiment, the present invention is further illustrated, but limited the present invention never in any form, and any conversion done based on training centre of the present invention or replacement, all belong to protection scope of the present invention.
Natural bionic technique based on cold chain of the present invention, comprises that pre-treatment, bionic extraction, separation are concentrated, sterilizing-drying step, specifically comprises:
A, pre-treatment: raw material fresh goods, quick frozen product, dried frozen aquatic products or dry product are pulverized through defibrination or broken wall, dried frozen aquatic products or dry product sheet carry out beating powder broken wall;
B, bionic extraction: insert in membrane bioreactor by the raw material fresh goods after pre-treatment, add the deionized water of raw material weight than 1:3 ~ 10, carries out bionic extraction 60 ~ 180min and obtain extract;
C, separation concentrate: extract dehydration after dialysis is separated concentrates and obtains concentrate;
D, sterilizing-drying: by concentrate quick-frozen extremely less than-20 DEG C after low temperature sterilization, then obtain the active elite of ecosystem through freeze drying.
The sporoderm-broken rate of broken wall described in step A is more than 90%.
Membrane bioreactor described in step B comprises pot type and channel-type membrane bioreactor, to simulate structural form and the digestion pattern of stomach and enteron aisle.
The temperature of the bionic extraction described in step B be physics bionical be less than 35 DEG C, physics biochemical bionical be less than 45 DEG C.
In A, B, step C, process temperature all controls below 45 DEG C.
Sublimation temperature < in freeze drying described in D step-18 DEG C.
Application of the present invention is ecosystem active elite, Chinese medicine fresh medicine preparation or active Chinese medicine instant decoction pieces based on the product of the natural bionic explained hereafter of cold chain; It is applied as pharmaceutical industry and extracts application in the low temperature preextraction of effective active monomer further.
The present invention will be further described for lower mask body case study on implementation:
Embodiment 1
With fresh matsutake 100kg, enter membrane bioreactor, add deionized water 300kg after low temperature defibrination, start physics bionic extraction below 15 DEG C, the time is 75 minutes, and Extracting temperature is < 15 DEG C; Extract dehydration after dialysis is separated is concentrated, obtains 10kg concentrate; Concentrate is quick-frozen immediately extremely-40 DEG C after low-temperature ultrahigh-pressure sterilization.Obtain the primary elite of 1.4kg matsutake through freeze drying again, then dress up 1 gram of dress through nitrogen package, do not added the active elite pulvis of matsutake ecosystem of any additive.
Adopt the relative amount that gas-chromatography-mass spectrum interlock method (GC-MS) measures Fructificatio Tricholomatis Matsutakis extract volatile oil content and matsutake characteristic perfume composition 1-octene-3-alcohol, methyl cinnamate accounts for total volatile oil, as follows:
Note: traditional handicraft Fructificatio Tricholomatis Matsutakis extract refers to matsutake through hot reflux water extraction, the dried powder that vacuum decompression drying concentrates and vacuum drying obtains.
The matsutake ecosystem elite that visible present invention process extracts comparatively retains the lifting of the essence of odor characteristic composition compared with traditional handicraft, it is particularly the most difficultly in water extraction in plant extract extract the part retained that the fragrance ingredient of plant extracts, so the complete extraction of ecosystem fragrance ingredient and enrichment in extract, can illustrate that other active ingredients also obtain simultaneously and more completely extract and enrichment.
Embodiment 2
With fresh leaf of Moringa 30kg, after defibrination, enter membrane bioreactor.Add deionized water 90kg and start physics bionic extraction, the time is 65 minutes, and temperature is lower than 20 degree.Extract dehydration after dialysis is separated and is centrifugal is concentrated, obtains 9kg concentrate, and concentrate quick-frozen immediately after cold sterilization, to less than-40 DEG C, then obtains 1.8kg freeze-dried powder after freeze drying, and the active elite tablet of Moringa ecosystem is made in freeze-drying after pressed powder.
Analyze with HP-7890A-5975CGC-MS combined gas chromatography mass spectrometry.
The sample volatile oil content of the primary elite of the Moringa that embodiment 2 prepares is >=0.06%, and the fresh leaf of raw material is enriched volatile aroma oil composition for >=0.02%. illustrates in bionic extraction process.
The primary elite of Moringa is by the separation accuracy in control biomimetics leaching process, and also more intact remains the chlorophyll of Moringa, makes extract color or bottle green.
Embodiment 3
With the fresh agate card of 10kg, add 10kg deionized water under cleaning rear less than 20 DEG C cryogenic conditions, then use paste mill grinding, then enter membrane bioreactor, add 40kg deionized water and carry out the biochemical bionic extraction of primary physical.Regulate PH to 6.5 with acetic acid, then add each 0.2 gram of two kinds of Bifidobacterium freeze-dried powders, start to react, the reaction time is 135 minutes, and reaction temperature is 38 DEG C.Then clarification, separation, impurity removal and thickening, obtain concentrate 7.5kg.Concentrate is with after low-temperature ultrahigh-pressure sterilizing, and by concentrate quick-frozen immediately to less than-40 DEG C, then freeze drying, obtains 800g dried powder.The active elite pulvis of agate card ecosystem is made again after processing and packing.
Detect with HP-7890A-5975CGC-MS combined gas chromatography mass spectrometry analysis.
The content of the active elite benzene acetonitrile of agate card ecosystem that embodiment 3 prepares and benzyl isothiocyanate accounts for the ratio that glucosinolate decomposes volatile oil total amount:
Note: traditional handicraft agate card extract refers to that getting final vacuum reduction vaporization by hot reflux water extraction concentrates, then with the agate card extract obtained after vacuum microwave drying.
Visible traditional extraction technique decomposes owing to technique hindering the natural forward of agate card glucosinolate, so the benzyl isothiocyanate of the benzene acetonitrile that its analyte is major part to be harmful to and small part.And embodiment 3 allows the glucosinolate of agate card utilize the myrosin of self natural decomposition under bionical condition by natural bionic technique, just can generate most effective ingredient benzyl isothiocyanate, and only have the oxious component benzene acetonitrile of small part.
Embodiment 4
With fresh bitter bamboo bar 30kg, after broken fine pulverizing, defibrination, enter membrane bioreactor physics bionic extraction, add water 300kg, and extraction time is 65 minutes, Extracting temperature < 20 DEG C.Extract obtains 18kg concentrate after clarification and thickening, concentrate is quick-frozen immediately extremely less than-40 DEG C after low-temperature ultrahigh-pressure sterilization, freeze drying becomes freeze-dried powder again, and freeze-dried powder becomes active fresh bamboo sap elite after packaging, then processing and packing makes the fresh medicine powder of fresh bamboo sap further.
Rush one glass of water with fresh bamboo sap pulvis, can smell the delicate fragrance of bamboo immediately, soup look is yellow green, the micro-hardship of taste and sweet mouthfeel.The fresh bamboo sap aqua of pharmacy's sale, coffee-like on the market, without fragrance, only has ointment taste.
Traditional Chinese Medicine is thought, fresh bamboo sap should be oral with fresh medicine, has remarkable efficacy to clearing lung-heat, the pipe that purifies the blood.But due to the problem of preservation technique, the fresh bamboo sap of current commercial type has not been fresh medicine, but the aqua after being endured by high-temperature boiling, drug activity reduces greatly.
The fresh medicine powder of fresh bamboo sap that present invention process is produced, remains the active ingredient of new fresh bamboo sap in good condition under cold chain condition, and solves storage, carries and the problem of instructions of taking.
Embodiment 5
Get fresh root of kudzu vine 30kg, after cleaning section defibrination, be separated and remove long fibre, then add 120kg deionized water, enter membrane bioreactor with the circulation cold reflux extracting and developing that dialysis is separated and enrichment facility is formed and concentrate.For preventing root of kudzu vine effective ingredient to be oxidized, the basin machine container of whole process engineering carries out nitrogen packing from oxygen.Concentrate, after cold sterilization, is refrigerated to less than-40 DEG C immediately, then obtains the active elite freeze-dried powder of the 0.61kg root of kudzu vine after freeze drying.Processing and packing makes the fresh medicine powder of the root of kudzu vine further again.
Detect with HP-7890A-5975CGC-MS combined gas chromatography mass spectrometry analysis, the Constituents of The Essential Oil of root of kudzu vine fresh medicine preparation is 1.6%, and the Constituents of The Essential Oil of the fresh root of kudzu vine is 0.1%, illustrate that root of kudzu vine fresh medicine preparation Constituents of The Essential Oil on the basis being enriched with effective constituent that present invention process is produced also is enriched 16 times.Same method analyzes the kudzu-vine root powder prepared slices of Chinese crude drugs and the kudzu root extract of commercial type, and its volatile oil content does not detect.
Fragrance and the mouthfeel of the mouthfeel of root of kudzu vine elite pulvis after brewing water and fresh squeezing kudzuvine root juice are substantially identical, and the kudzu-vine root powder medicine materical crude slice of commercial type and kudzu root extract alternative tea do not have mouthfeel and the fragrance of the root of kudzu vine substantially.
Embodiment 6
Employing is propagated Africa large Lian 10 kilograms, the 30kg that adds water after cleaning artificially and to be pulled an oar pulverizing, then adds 30kg deionized water and enter membrane bioreactor physics bionic extraction, Extracting temperature <12 DEG C, and the time is 60 minutes.Extract dehydration after clarification is concentrated, and obtain 7kg concentrate, concentrate is quick-frozen immediately after cold sterilization, then after freeze drying, obtains 780 grams of freeze-dried powders, makes capsule fresh medicine preparation after encapsulated by 0.5 gram/.
Contrast sample is American cockroach water-soluble extractive, with the first drying material of tradition, and pulverizes, then lixiviate, removing impurities final vacuum evaporation and concentration, drier through low-temperature reduced-pressure, obtains American cockroach traditional handicraft extract control sample.
American-cockroach-extract activity experiment is analyzed
Experiment mice: kunming mice 90, body weight 18g ± 2g, purchased from unming Medical College's Experimental Animal Center.
Experimental technique: American cockroach activity extract and traditional handicraft control sample extract are dissolved in deionized water, by 120mg/kg gavage, I group of 30 gavage activity extract, II group of 30 gavages tradition contrast water extract, blank sample 30 gavage physiological saline 1ml.Continuous gavage blood test white blood cell count(WBC) after 7 days.
Project Blank I group II group 5-->
White blood cell count(WBC) (10 9/L ） 4.51 5.28 4.74
From white blood cell count(WBC) statistics, white blood cell count(WBC) activity extract improves 17.1%, and contrast traditional handicraft extract improves 5.1%, and enhancing rate present invention process extract is more than 3 times of traditional handicraft extract.
Embodiment 7
Get 10kg broken black tea, after pulverizing further, add 90kg deionized water enter membrane bioreactor bionic extraction, Extracting temperature < 45 DEG C, the time is 85 minutes.Extract obtains 38kg concentrate after being separated and concentrating, and concentrate, again through freeze drying, obtains 2.1kg xeraphium, and xeraphium is packaged as 0.5 gram of dress bar bag, makes original flavor instant black tea.
With HP-7890A-5975CGC-MS gas chromatography mass spectrometry method analysis inspection, the volatile aroma oil composition checking former tea is 9mg/100g, and the fragrance ingredient of extract instant black tea is 33mg/100g.The fragrance ingredient of black tea retains intact and obtains enrichment, and the fragrance ingredient inspection of the instant black tea that tradition is sold on the market is less than 3mg/100g.
Embodiment 8
With the fresh pseudo-ginseng of the equal size in a collection of place of production, 10 kilograms are made as freeze drying product, and 10 kilograms are made as low temperature drying product.Freeze-drying pseudo-ginseng 10 kilograms, add 100kg deionized water after being crushed to 100 orders and enter membrane bioreactor physics bionic extraction, Extracting temperature < 20 DEG C, the time is 90 minutes.Extract the concentrated 12kg that obtains after being separated and concentrating and extract concentrate, this step as preextraction, then obtains the Active pseudo-ginseng total saposins that 855g purity is more than 90% after chromatographic isolation.
Get 10 kilograms and dry pseudo-ginseng, use alcohol steep after being broken for particle, extract filters through 0.2um, obtains in evaporation drying after chromatographic column separation and purification the arasaponin that purity 610g is more than 87%.
Use Shimadzu LC-10AT vPcompound components of panax notoginseng (Rg in dry thing is extracted in high-efficient liquid phase chromatogram discuss inspection 1+ Rb 1+ R 1) total content is for the mass percent of raw material, results of comparison is as follows:
Same batch sample Extraction process （R g 1+Rb 1+R 1) % extract effective ingredient yield
Fresh pseudo-ginseng Cold chain natural bionic technique 6.96%
Dry pseudo-ginseng Alcohol steep, evaporation drying 5.32%
The new technology that visible freeze-drying pseudo-ginseng makes raw material extracts the extraction of more dry pseudo-ginseng traditional handicraft, and effective ingredient yield improves 30.8%, has and improves meaning significantly.
Embodiment 9
With drying Radix Isatidis 10kg, add 100kg deionized water after being crushed to 80 orders and enter membrane bioreactor physics bionic extraction, Extracting temperature < 35 DEG C, the time is 85 minutes.Extract obtains dry cream after being separated thickener freeze drying.Contrast method gets 10 kilograms of Radix Isatidis water boiling and precipitation with ethanol methods extractions of same batch, then obtains dry cream through evaporating, concentrating and drying.Can be used as the Radix Isatidis active instant prepared slices of Chinese crude drugs with the isatis root extract that present invention process is produced.
Detecting Content Determination of Indirubin in dry cream with high performance liquid chromatograph is
Technique Content Determination of Indirubin (ug/g)
Cold chain natural bionic 9.86
Water boiling and precipitation with ethanol method 0.84
Visible present invention process has the raising of matter compared with traditional handicraft to the reservation of thermal sensitivity effective ingredient indigo red.
Embodiment 10
Get fresh stem of noble dendrobium 2kg, pulverize defibrination after cleaning, add 20kg deionized water and enter membrane bioreactor physics bionic extraction, Extracting temperature < 25 DEG C, extract carries out freeze drying after being separated and concentrating, and obtains 75g primary extract freeze-dried powder.This freeze-dried powder is substantially namely water-soluble, can be used as the stem of noble dendrobium fresh medicine instant decoction pieces electuary.
Control sample carries out water extraction with getting 1kg after a collection of stem of noble dendrobium oven drying, and water extract evaporation drying after 1um filters obtains the dry thing of 95g, is similarly Herba Dendrobii extract medicine materical crude slice electuary.The dendrobium polysaccharide of inspection two extracts and alkaloid, contrast as follows:
Project Dendrobium polysaccharide (%) Alkaloid (%)
The stem of noble dendrobium fresh medicine instant decoction pieces electuary 39.6 0.065
The common stem of noble dendrobium fresh medicine instant decoction pieces electuary 28.7 0.048
The fresh medicine instant decoction pieces of the stem of noble dendrobium that visible present invention process extracts significantly improves compared with the effective active components content of the stem of noble dendrobium instant decoction pieces of traditional handicraft.
Embodiment 11
With wild fresh Suillus albidipes (Peck) Sing (delicious beef liver) 100kg, membrane bioreactor is entered after low temperature defibrination, add deionized water 300kg, physics bionic extraction is started below 15 DEG C, time is 75 minutes, extract dehydration after dialysis is separated and is centrifugal is concentrated, obtains 10kg concentrate, and concentrate quick-frozen immediately after low temp. electric pulse sterilizing is kept in-40 DEG C; Carrying out freeze drying by sending into freeze drying storehouse after ice cube fragmentation again, obtaining the primary elite of the delicious beef liver of 1.28kg, then dressing up 1 gram/bag of dress through nitrogen package, do not added the ecosystem delicious beef liver elite pulvis of any additive.
The extraction of control sample heats lixiviate with a collection of cleaning section and oven dry deionized water, and extract reduction vaporization after 0.2um filters is concentrated obtains medicinal extract, and medicinal extract obtains delicious beef liver tradition water extract through vacuum microwave drying again.
Animal used as test: Kunming mouse 120, body weight 18g ± 2g, purchased from unming Medical College's Experimental Animal Center.
Animal is divided into three groups: often organize 40, and start test after within continuous three days, carrying out low dose irradiation, KB group is blank group, and I group is present invention process natural bionic extract group, and II group is contrast traditional handicraft water extract group.By 80mg/kg gavage, control group gavage 1mL physiological saline, respectively after experiment starts 7,14,21d mouse tail arteries and veins blood sampling 20uL, put in dry dilution, with blood count instrument count blood cells number, count results often group is averaged.
Conclusion: visible natural bionic process activity extract has larger impact compared with the recovery lifting of traditional handicraft extract on mouse immune mechanism, is obviously better than traditional extract.
Embodiment 12
Use 30kg fresh ginger, after low temperature defibrination, add 200kg deionized water put into membrane bioreactor, extraction and isolation and concentrated, temperature < 35 DEG C.Last concentrate obtains 600g freeze-dried powder after quick-frozen and freeze drying, and in the dry decontamination of temperature lower than 40 DEG C, nitrogen envelope is packaged as 2g/ bag, obtains the active elite pulvis of fresh ginger.Be applicable as flavouring or health products.
It is pungent that the active elite mouthfeel of fresh ginger comparatively decocts ginger decoction, and excitant pungent wants much strong, and gingerol content is several times as much as decoction ginger decoction.
Anti-inflammatory, antibacterial and dehumidifying remove cold effect larger lifting.

Claims (9)

1. based on a natural bionic technique for cold chain, it is characterized in that comprising that pre-treatment, bionic extraction, separation are concentrated, sterilizing-drying step, specifically comprise:
A, pre-treatment: raw material fresh goods, quick frozen product, dried frozen aquatic products or dry product are pulverized through defibrination or broken wall, dried frozen aquatic products or dry product sheet carry out beating powder broken wall;
B, bionic extraction: insert in membrane bioreactor by the raw material fresh goods after pre-treatment, add the deionized water of raw material weight than 1:3 ~ 10, carries out bionic extraction 60 ~ 180min and obtain extract;
C, separation concentrate: extract dehydration after dialysis is separated concentrates and obtains concentrate;
D, sterilizing-drying: by concentrate quick-frozen extremely less than-20 DEG C after low temperature sterilization, then obtain the active elite of ecosystem through freeze drying.
2. the natural bionic technique based on cold chain according to claim 1, is characterized in that the sporoderm-broken rate of broken wall described in step A is more than 90%.
3. the natural bionic technique based on cold chain according to claim 1, is characterized in that the membrane bioreactor described in step B comprises pot type and channel-type membrane bioreactor, to simulate structural form and the digestion pattern of stomach and enteron aisle.
4. the natural bionic technique based on cold chain according to claim 1, it is characterized in that the bionic extraction described in step B can be divided into that physics is bionical or physics is biochemical bionical, physics bionic extraction temperature is less than 35 DEG C, and the biochemical bionic extraction temperature of physics is less than 45 DEG C.
5. the natural bionic technique based on cold chain according to claim 1, is characterized in that in A, B, step C, process temperature all controls below 45 DEG C.
6., according to claim 1 or 5 based on the natural bionic technique of cold chain, it is characterized in that the mobility raw material in step C after dialysis separation and the water after thickening are back to membrane bioreactor retort, realize cold reflux circulation and extract.
7. the natural bionic technique based on cold chain according to claim 1, is characterized in that the sublimation temperature <-18 DEG C in the freeze drying described in D step.
8. a new product for the arbitrary described natural bionic explained hereafter based on cold chain of claim 1 ~ 7, the new product of described production is biological ecosystem active elite, Chinese medicine fresh medicine preparation or active Chinese medicine instant decoction pieces.
9. an application for the arbitrary described natural bionic technique based on cold chain of claim 1 ~ 7, is characterized in that the described natural bionic technique based on cold chain is extracting the application in the low temperature preextraction technique of biologically active active ingredient monomer further.
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