CN105455141A - Original ecological Moringa oleifera leaf active essence as well as preparation method and preparations thereof - Google Patents
Original ecological Moringa oleifera leaf active essence as well as preparation method and preparations thereof Download PDFInfo
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- CN105455141A CN105455141A CN201510879081.1A CN201510879081A CN105455141A CN 105455141 A CN105455141 A CN 105455141A CN 201510879081 A CN201510879081 A CN 201510879081A CN 105455141 A CN105455141 A CN 105455141A
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- leaf
- moringa
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- ecosystem
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- 235000011347 Moringa oleifera Nutrition 0.000 title claims abstract description 74
- 238000002360 preparation method Methods 0.000 title claims abstract description 22
- 244000179886 Moringa oleifera Species 0.000 title abstract 7
- 238000000605 extraction Methods 0.000 claims abstract description 24
- 239000011664 nicotinic acid Substances 0.000 claims abstract description 23
- 239000002994 raw material Substances 0.000 claims abstract description 18
- 238000000926 separation method Methods 0.000 claims abstract description 17
- 238000001035 drying Methods 0.000 claims abstract description 11
- 239000000843 powder Substances 0.000 claims abstract description 10
- 239000000463 material Substances 0.000 claims abstract description 9
- 238000000034 method Methods 0.000 claims abstract description 8
- 230000001954 sterilising effect Effects 0.000 claims abstract description 7
- 238000004659 sterilization and disinfection Methods 0.000 claims abstract description 7
- 239000003826 tablet Substances 0.000 claims abstract description 7
- 239000002775 capsule Substances 0.000 claims abstract description 6
- 239000008187 granular material Substances 0.000 claims abstract description 5
- 239000006187 pill Substances 0.000 claims abstract description 5
- 241000220215 Moringa Species 0.000 claims description 67
- 239000012141 concentrate Substances 0.000 claims description 15
- 239000012528 membrane Substances 0.000 claims description 14
- 238000002203 pretreatment Methods 0.000 claims description 14
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 13
- 239000008367 deionised water Substances 0.000 claims description 12
- 229910021641 deionized water Inorganic materials 0.000 claims description 12
- 239000002002 slurry Substances 0.000 claims description 10
- 238000004108 freeze drying Methods 0.000 claims description 8
- 239000000203 mixture Substances 0.000 claims description 7
- 238000005352 clarification Methods 0.000 claims description 6
- 230000018044 dehydration Effects 0.000 claims description 6
- 238000006297 dehydration reaction Methods 0.000 claims description 6
- 239000012535 impurity Substances 0.000 claims description 5
- 238000004140 cleaning Methods 0.000 claims description 3
- 238000005119 centrifugation Methods 0.000 claims description 2
- 230000029087 digestion Effects 0.000 claims description 2
- 210000002784 stomach Anatomy 0.000 claims description 2
- 238000000859 sublimation Methods 0.000 claims description 2
- 230000008022 sublimation Effects 0.000 claims description 2
- 239000003814 drug Substances 0.000 abstract description 4
- 239000000341 volatile oil Substances 0.000 abstract description 4
- 238000007781 pre-processing Methods 0.000 abstract 2
- 239000003921 oil Substances 0.000 description 5
- 230000000694 effects Effects 0.000 description 4
- 239000011782 vitamin Substances 0.000 description 3
- 235000013343 vitamin Nutrition 0.000 description 3
- 229930003231 vitamin Natural products 0.000 description 3
- 229940088594 vitamin Drugs 0.000 description 3
- 150000003722 vitamin derivatives Chemical class 0.000 description 3
- FPIPGXGPPPQFEQ-OVSJKPMPSA-N all-trans-retinol Chemical compound OC\C=C(/C)\C=C\C=C(/C)\C=C\C1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-OVSJKPMPSA-N 0.000 description 2
- 239000008280 blood Substances 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 238000000502 dialysis Methods 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 238000002290 gas chromatography-mass spectrometry Methods 0.000 description 2
- 230000036039 immunity Effects 0.000 description 2
- 239000000523 sample Substances 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 1
- 208000001953 Hypotension Diseases 0.000 description 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- 244000269722 Thea sinensis Species 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 235000019169 all-trans-retinol Nutrition 0.000 description 1
- 239000011717 all-trans-retinol Substances 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 235000013361 beverage Nutrition 0.000 description 1
- 230000003592 biomimetic effect Effects 0.000 description 1
- 230000036772 blood pressure Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 229930002875 chlorophyll Natural products 0.000 description 1
- 235000019804 chlorophyll Nutrition 0.000 description 1
- ATNHDLDRLWWWCB-AENOIHSZSA-M chlorophyll a Chemical compound C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC(C(CC)=C3C)=[N+]4C3=CC3=C(C=C)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 ATNHDLDRLWWWCB-AENOIHSZSA-M 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 239000013068 control sample Substances 0.000 description 1
- 239000006071 cream Substances 0.000 description 1
- 235000005911 diet Nutrition 0.000 description 1
- 230000037213 diet Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000004821 distillation Methods 0.000 description 1
- 239000002552 dosage form Substances 0.000 description 1
- 230000002124 endocrine Effects 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 239000003205 fragrance Substances 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 208000021822 hypotensive Diseases 0.000 description 1
- 230000001077 hypotensive effect Effects 0.000 description 1
- 238000002386 leaching Methods 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 231100000957 no side effect Toxicity 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 235000008935 nutritious Nutrition 0.000 description 1
- 239000002304 perfume Substances 0.000 description 1
- -1 pulvis Substances 0.000 description 1
- 238000010992 reflux Methods 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 230000000087 stabilizing effect Effects 0.000 description 1
- 239000003053 toxin Substances 0.000 description 1
- 231100000765 toxin Toxicity 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Abstract
The invention discloses original ecological Moringa oleifera leaf active essence as well as a preparation method and preparations thereof. According to the original ecological Moringa oleifera leaf active essence, fresh Moringa oleifera leaves are taken as raw materials and subjected to pre-processing, bionic extraction, separation, concentration, sterilization, drying and the like, the original ecological Moringa oleifera leaf active essence is obtained, and the content of aroma volatile oil in the original ecological Moringa oleifera leaf active essence is larger than or equal to 0.04%. The method comprises steps as follows: pre-processing, bionic extraction, separation, concentration, sterilization and drying. The preparations are powder, tablets, granules, capsules and pills and are prepared from the original ecological Moringa oleifera leaf active essence and auxiliary materials which are acceptable in medicine and added to the essence. The whole process is completed under a cold chain condition and is a natural bionic process based on the cold chain.
Description
Technical field
The invention belongs to technical field of food deep processing, belong to vegetable active elite processing technique field further, be specifically related to active elite of a kind of leaf of Moringa ecosystem and preparation method thereof and preparation.
Background technology
Moringa is wild originates in Kenya and Ethiopia, and India's plantation Moringa has the history of more than 40 years, and the Moringa that China introduces a fine variety in the torrid areas such as Xishuangbanna and Yuanyang is comparatively successful.
Leaf of Moringa is decided to be new resource food by China at present, and it has abundant nutrition.Alphalin in India's tradition Moringa fresh leaves, the content of B, C, E are very abundant, can become natural children's vitamin or proteinaceous nutrient replenishers.
Moringa has good effect to control blood sugar, hypotensive, reducing blood lipid, also can improve immunity, prevent disease, improving water flood, delay senility, toxin expelling and enhance metabolism, regulate endocrine.
Moringa seeds is the life diet of natural fresh-keeping, eats something rare best results, but the maximum leaf of Moringa of output only has cured leaf powder at present on the market.Plant once many active ingredients after dry, as vitamin, ferment just have larger loss.Therefore, the Moringa product developing a kind of energy retentive activity composition is very important.
Summary of the invention
The first object of the present invention is to provide a kind of leaf of Moringa ecosystem active elite; Second object is to provide the preparation method of the described active elite of leaf of Moringa ecosystem; 3rd object is to provide the preparation of the described active elite of leaf of Moringa ecosystem.
The first object of the present invention realizes like this, for raw material with the fresh leaf of leaf of Moringa,, sterilizing-drying step concentrated through pre-treatment, bionic extraction, separation prepares the active elite of leaf of Moringa ecosystem, volatile aroma oil composition >=0.04% in the active elite of described leaf of Moringa ecosystem.
The second object of the present invention is achieved in that and comprises that pre-treatment, bionic extraction, separation are concentrated, sterilizing-drying step, specifically comprises:
A, pre-treatment: by for subsequent use after defibrination for the deionized water adding weight ratio 1 ~ 8 times after fresh for raw material leaf of Moringa leaf cleaning;
B, bionic extraction: fresh for the raw material leaf of Moringa after pre-treatment leaf slurry is inserted in membrane bioreactor dynamic circulation extractor, add the deionized water of the fresh leaf slurry weight of raw material leaf of Moringa than 3 ~ 10 times, carry out bionic extraction 60 ~ 120min in less than 35 DEG C and obtain extract;
C, separation concentrate: extract dehydration after clarification, separation, impurity removal is concentrated obtains concentrate;
D, sterilizing-drying: by concentrate quick-frozen extremely less than-20 DEG C after low temperature sterilization, then obtain the active elite of object leaf of Moringa ecosystem through freeze drying.
The third object of the present invention is achieved in that adding pharmaceutically acceptable auxiliary material in the active elite of described leaf of Moringa ecosystem is prepared into powder, tablet, granule, capsule and pill.
The active elite (i.e. extract) of leaf of Moringa ecosystem of the present invention remains fragrance and the characteristic flavor on basis of the fresh leaf of Moringa, the activity of vitamin and ferment is remained under cold chain condition, Moringa ecosystem active elite volatile aroma oil composition >=0.04%, the active elite of leaf of Moringa ecosystem of the present invention can be used as the dosage form such as pulvis, tablet, capsule, the active elite solid beverage of ecosystem or alternative tea, or as active Chinese medicine instant decoction pieces, Chinese medicine fresh medicine preparation; Can be used as the natural nutriment of children or the elderly's nutritious supplementary pharmaceutical or three high patient's informal dresses, there is the effect of falling three height, stabilizing blood pressure, short sleep, raising immunity, enhancing vitality, and long-term taking has no side effect.
Advantage of the present invention:
(1) whole technique completes under cold chain condition, and maximum temperature is no more than 35 DEG C.
(2) whole extracting and developing, concentration technology form a dynamic circulation cold reflux extraction system by membrane bioreactor, dialysis separator and enrichment facility.It is a bionic artistic based on cold chain.
Detailed description of the invention
Below in conjunction with embodiment, the present invention is further illustrated, but limited the present invention never in any form, and any conversion done based on training centre of the present invention or replacement, all belong to protection scope of the present invention.
The active elite of leaf of Moringa ecosystem of the present invention, for raw material with the fresh leaf of leaf of Moringa,, sterilizing-drying step concentrated through pre-treatment, bionic extraction, separation prepares the active elite of leaf of Moringa ecosystem, volatile aroma oil composition >=0.04% in the active elite of described leaf of Moringa ecosystem.
The preparation method of the active elite of leaf of Moringa ecosystem of the present invention, comprises that pre-treatment, bionic extraction, separation are concentrated, sterilizing-drying step, specifically comprises:
A, pre-treatment: by for subsequent use after defibrination for the deionized water adding weight ratio 1 ~ 8 times after fresh for raw material leaf of Moringa leaf cleaning;
B, bionic extraction: fresh for the raw material leaf of Moringa after pre-treatment leaf slurry is inserted in membrane bioreactor dynamic circulation extractor, add the deionized water of the fresh leaf slurry weight of raw material leaf of Moringa than 3 ~ 10 times, carry out bionic extraction 60 ~ 120min in less than 35 DEG C and obtain extract;
C, separation concentrate: extract dehydration after clarification, separation, impurity removal is concentrated obtains concentrate;
D, sterilizing-drying: by concentrate quick-frozen extremely less than-20 DEG C after low temperature sterilization, then obtain the active elite of object leaf of Moringa ecosystem through freeze drying.
The temperature of defibrination described in step A controls below 20 DEG C.
Membrane bioreactor described in step B comprises pot type and channel-type membrane bioreactor, to simulate structural form and the digestion pattern of stomach and enteron aisle.
Described bionic extraction be by pre-treatment after the fresh leaf slurry of raw material leaf of Moringa insert in the dynamic circulation extractor of membrane bioreactor, add the deionized water of the fresh leaf slurry weight of raw material leaf of Moringa than 3 ~ 10 times again, carry out bionic extraction 60 ~ 120min in less than 35 DEG C and obtain extract.
Clarification described in step C and separation, impurity removal be adopt filter, the combination of one or more methods of UF membrane or centrifugation.
The temperature that dehydration described in step C concentrates controls below 35 DEG C.
Sublimation temperature < in freeze drying described in D step-18 DEG C.
The preparation of the active elite of leaf of Moringa ecosystem of the present invention is add pharmaceutically acceptable auxiliary material in the described active elite of leaf of Moringa ecosystem to be prepared into powder, tablet, granule, capsule and pill.
Embodiment 1
With fresh leaf of Moringa 30kg, after defibrination, enter membrane bioreactor.Add deionized water 90kg and start physics bionic extraction, the time is 65 minutes, and temperature is lower than 20 degree.Extract dehydration after dialysis is separated and is centrifugal is concentrated, obtains 9kg concentrate, and concentrate quick-frozen immediately after cold sterilization, to less than-40 DEG C, then obtains 1.8kg freeze-dried powder after freeze drying, and the active elite tablet of Moringa ecosystem is made in freeze-drying after pressed powder.
Analyze with HP-7890A-5975CGC-MS combined gas chromatography mass spectrometry.
The sample volatile oil content of the primary elite of the Moringa that embodiment 1 prepares is >=0.06%, and the fresh leaf of raw material is enriched volatile aroma oil composition for >=0.02%. illustrates in bionic extraction process.
The primary elite of Moringa is by the separation accuracy in control biomimetics leaching process, and also more intact remains the chlorophyll of Moringa, makes extract color or green.
Embodiment 2
With fresh leaf of Moringa 30kg, add 10kg deionized water, below 20 DEG C, the membrane bioreactor dynamic extraction pot of low temperature bionic extraction is adding 90kg deionized water, extraction time is 120 minutes, temperature is lower than 35 DEG C, and extract obtains 12kg concentrate after being separated and concentrating, and concentrate quick-frozen immediately after normal temperature ultrahigh-pressure sterilization is kept in less than-20 DEG C, then enter freeze drying and obtain 2.2kg freeze-dried powder, after being packed by freeze-dried powder, make 1 gram/bag of dress pulvis.
GC-MS chromatography of gases the MS identical by embodiment 1 is analyzed, its volatile aroma oil composition >=0.08%.
Embodiment 3(traditional handicraft control sample)
With dry leaf of Moringa 10kg, add 50kg deionized water circumfluence distillation 90 minutes, extract, through clarification filtration final vacuum reduced pressure concentration, obtains concentrated extract, then through micro-wave vacuum, obtains 1.6kg extract xeraphium.
Analytical method GC-the MS identical with example 1 analyzes, Moringa cured leaf sample volatile oil content >=0.01%, Moringa extract volatile oil content < 0.01%.
Leaf of Moringa tradition extract is without the characteristic perfume of leaf of Moringa, and only have cream taste, color becomes brown.
Embodiment 4
The active elite of the Moringa that Example 1 prepares adds pharmaceutically acceptable auxiliary material and is prepared into powder.
Embodiment 5
The active elite of the Moringa that Example 2 prepares adds pharmaceutically acceptable auxiliary material and is prepared into tablet.
Embodiment 6
The active elite of the Moringa that Example 2 prepares adds pharmaceutically acceptable auxiliary material and is prepared into granule.
Embodiment 7
The active elite of the agate card that Example 2 prepares adds pharmaceutically acceptable auxiliary material and is prepared into capsule.
Embodiment 8
The active elite of the Moringa that Example 1 prepares adds pharmaceutically acceptable auxiliary material and is prepared into pill.
Claims (9)
1. the active elite of leaf of Moringa ecosystem, it is characterized in that with the fresh leaf of leaf of Moringa for raw material,, sterilizing-drying step concentrated through pre-treatment, bionic extraction, separation prepares the active elite of leaf of Moringa ecosystem, volatile aroma oil composition >=0.04% in the active elite of described leaf of Moringa ecosystem.
2. a preparation method for the active elite of leaf of Moringa ecosystem according to claim 1, is characterized in that comprising that pre-treatment, bionic extraction, separation are concentrated, sterilizing-drying step, specifically comprises:
A, pre-treatment: by for subsequent use after defibrination for the deionized water adding weight ratio 1 ~ 8 times after fresh for raw material leaf of Moringa leaf cleaning;
B, bionic extraction: fresh for the raw material leaf of Moringa after pre-treatment leaf slurry is inserted in membrane bioreactor dynamic circulation extractor, add the deionized water of the fresh leaf slurry weight of raw material leaf of Moringa than 3 ~ 10 times, carry out bionic extraction 60 ~ 120min in less than 35 DEG C and obtain extract;
C, separation concentrate: extract dehydration after clarification, separation, impurity removal is concentrated obtains concentrate;
D, sterilizing-drying: by concentrate quick-frozen extremely less than-20 DEG C after low temperature sterilization, then obtain the active elite of object leaf of Moringa ecosystem through freeze drying.
3. preparation method according to claim 2, is characterized in that the temperature of defibrination described in step A controls below 20 DEG C.
4. preparation method according to claim 2, is characterized in that the membrane bioreactor described in step B comprises pot type and channel-type membrane bioreactor, to simulate structural form and the digestion pattern of stomach and enteron aisle.
5. preparation method according to claim 2, it is characterized in that described bionic extraction be by pre-treatment after the fresh leaf slurry of raw material leaf of Moringa insert in the dynamic circulation extractor of membrane bioreactor, add the deionized water of the fresh leaf slurry weight of raw material leaf of Moringa than 3 ~ 10 times again, carry out bionic extraction 60 ~ 120min in less than 35 DEG C and obtain extract.
6. preparation method according to claim 2, it is characterized in that the clarification described in step C and separation, impurity removal be adopt filter, the combination of one or more methods of UF membrane or centrifugation.
7. preparation method according to claim 2, is characterized in that the temperature that the dehydration described in step C concentrates controls below 35 DEG C.
8. preparation method according to claim 2, is characterized in that the sublimation temperature <-18 DEG C in the freeze drying described in D step.
9. a preparation for the active elite of leaf of Moringa ecosystem according to claim 1, is characterized in that adding pharmaceutically acceptable auxiliary material in the active elite of described leaf of Moringa ecosystem is prepared into powder, tablet, granule, capsule and pill.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510879081.1A CN105455141A (en) | 2015-12-05 | 2015-12-05 | Original ecological Moringa oleifera leaf active essence as well as preparation method and preparations thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510879081.1A CN105455141A (en) | 2015-12-05 | 2015-12-05 | Original ecological Moringa oleifera leaf active essence as well as preparation method and preparations thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105455141A true CN105455141A (en) | 2016-04-06 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510879081.1A Pending CN105455141A (en) | 2015-12-05 | 2015-12-05 | Original ecological Moringa oleifera leaf active essence as well as preparation method and preparations thereof |
Country Status (1)
| Country | Link |
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| CN (1) | CN105455141A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106617049A (en) * | 2016-09-29 | 2017-05-10 | 广州市汇吉科技企业孵化器有限公司 | Propolis healthcare product with moringa oleifera leaf extract and method for preparing propolis healthcare product |
| CN110037305A (en) * | 2019-05-28 | 2019-07-23 | 云南熊牌生物科技有限公司 | A kind of preparation method of moringa oleifera leaf extractive |
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| CN1895545A (en) * | 2006-06-13 | 2007-01-17 | 上海永昊生物医药研究所 | Glossy ganoderma spore essential oil compound capsules and preparation thereof |
| CN101156709A (en) * | 2007-11-14 | 2008-04-09 | 四川大学 | Apple normal juice zymolysis process technology |
| CN102894444A (en) * | 2012-10-15 | 2013-01-30 | 康美药业股份有限公司 | Ginseng functional drink, preparation method of ginseng functional drink and application of ginseng functional drink |
| CN103131195A (en) * | 2013-02-04 | 2013-06-05 | 云南茂昽生物科技有限公司 | Matsutake active extractives and preparation method and application thereof |
-
2015
- 2015-12-05 CN CN201510879081.1A patent/CN105455141A/en active Pending
Patent Citations (4)
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|---|---|---|---|---|
| CN1895545A (en) * | 2006-06-13 | 2007-01-17 | 上海永昊生物医药研究所 | Glossy ganoderma spore essential oil compound capsules and preparation thereof |
| CN101156709A (en) * | 2007-11-14 | 2008-04-09 | 四川大学 | Apple normal juice zymolysis process technology |
| CN102894444A (en) * | 2012-10-15 | 2013-01-30 | 康美药业股份有限公司 | Ginseng functional drink, preparation method of ginseng functional drink and application of ginseng functional drink |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106617049A (en) * | 2016-09-29 | 2017-05-10 | 广州市汇吉科技企业孵化器有限公司 | Propolis healthcare product with moringa oleifera leaf extract and method for preparing propolis healthcare product |
| CN110037305A (en) * | 2019-05-28 | 2019-07-23 | 云南熊牌生物科技有限公司 | A kind of preparation method of moringa oleifera leaf extractive |
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Application publication date: 20160406 |