CN104059157A - Ultrasonic extraction process of polygonatum sibiricum polysaccharides - Google Patents

Abstract

The invention relates to an ultrasonic extraction process of polygonatum sibiricum polysaccharides. The process comprises the steps of selecting rhizome of polygonatum sibiricum with the water content within 15%, then, putting the rhizome of polygonatum sibiricum into a screening machine to be screened thoroughly, and then putting into a smasher to be smashed to granules; putting granules of polygonatum sibiricum into a sealed container, vacuumizing, and then putting the container filled with the granules of polygonatum sibiricum into heating equipment to be heated; taking out the heated granules of polygonatum sibiricum, cooling to room temperature, and then, feeding the heated granules of polygonatum sibiricum to a grinder to be ground to powder; putting the powder of polygonatum sibiricum into an oven for roasting, wherein the set time is 30 seconds and the temperature is 110 DEG C; putting the roasted powder of polygonatum sibiricum into purified water at 40-60 DEG C to be immersed for 30 minutes, acting for 5-10 minutes at the ultrasonic power of 440W/L, carrying out ultrasonic extraction three times, filtering the filtrate, concentrating, adding ethanol, standing for 24 hours, dumping the supernate, reducing pressure, carrying out suction filtration, and drying to obtain polygonatum sibiricum polysaccharides. The process provided by the invention is short in extraction time, low in energy consumption and great in handling capacity of raw medicinal materials in a double or multiply manner.

Description

The ultrasonic extraction process of polygonatum polysaccharide

Technical field

The present invention relates to traditional Chinese medicine extraction technical field, be specifically related to a kind of ultrasonic extraction process of polygonatum polysaccharide.

Background technology

Polysaccharide (polysaccharide, PS) be one of the four large base substances that form life, extensively be present in higher plant, animal, microorganism, lichens and marine alga etc., as outer in born of the same parents in the born of the same parents of the shell fungi of the seed of plant, stem and leaf texture, animal mucus, insect and crustacean, bacterium etc.It claims again saccharan, is polymerized by glycosidic bond by 10 above monose molecules, and molecular weight is larger,, by even several ten thousand monose molecular compositions of hundreds of, is generally a class macromolecular cpd.Herbal polysaccharide is being brought into play biological action at aspects such as antitumor, anti-inflammatory, antiviral, hypoglycemic, anti-ageing, anticoagulation, immunological enhancement.Have the activity that its derivative of immunocompetent polysaccharide usually also has other, as sulfated polysaccharides has HIV (human immunodeficiency virus)-resistant activity and anticoagulant active, carboxymethylation polysaccharide has anti-tumor activity.Therefore the research and development of polysaccharide are more and more caused people's extensive concern.

Sealwort is Liliaceae Polygonatum per nnial herb, has sealwort, Yunnan sealwort and David's-harp etc., and its dry rhizome is used as medicine.Record according to traditional medicine is classical, sealwort belongs to rare Chinese medicine, has wide middle beneficial gas, enriching kidney essence, nourishing Yin and moistening lung, effect of the tonifying spleen of promoting the production of body fluid.Cure mainly dryness of the lung dry cough, body void weak,, the illnesss such as shortness of breath and palpitation, prolonged illness body fluid deficiency dry, diabetes, hypertension.All there is better effect to Cardiovarscular, tuberculosis, chronic hepatitis and at antibacterial, removing toxic substances, antifatigue, anti-ageing, hypoglycemic, reducing blood-fat, the aspect such as antitumor, can be used as medicine, can be used as again protective foods, since ancient times, people, using sealwort as the good merchantable brand of promoting longevity, are conventional tonic Chinese medicines.

In recent years, people deepen continuously to the research of Polygonatum plant, from this platymiscium, separate and obtain a lot of chemical compositions: polysaccharide, steroidal saponin, flavones etc., wherein polysaccharide has different physiological roles, mainly contain reducing blood-fat and study of anti-atherogenic effect, anti-aging effects, antitumor action, enhancing immunologic function, Anti HSV Action, fall the effects such as hypoglycemic effect, the necrosis of anti-hypoxia neurocyte and apoptotic effect, rise telomerase activation, thereby being in great demand to polygonatum polysaccharide.

To the extraction of polygonatum polysaccharide, general physics, the chemical process of adopting, by bulk drug pulverizing, degreasing, water, olefin(e) acid extracting again, after concentrated, remove foreigh protein removing and small molecular weight impurity, concentrate rear freeze-drying or precipitate and obtain polygonatum polysaccharide by organic solution, it is that the course of processing has high temperature that these methods exist a maximum problem, easily causes the destruction to effective constituent.

As the Chinese patent of application publication number: CN102351960A, " extracting method of polygonatum polysaccharide " disclosed, it is to adopt supersound extraction mode equally, and its step is comparatively loaded down with trivial details, and the polygonatum polysaccharide purity of extraction is not high, contains more impurity.

Summary of the invention

It is high that technical problem to be solved by this invention is to improve a kind of extraction efficiency, the ultrasonic extraction process of the polygonatum polysaccharide that purity is high.

Technical problem to be solved by this invention realizes by the following technical solutions:

A ultrasonic extraction process for polygonatum polysaccharide, comprises following methods step,

A) select the sealwort rhizome of water content in 15%, then sealwort rhizome is put into sifting machine, screening is clean, removes impurity, then puts into pulverizer and is ground into particulate state, and particle diameter, at 0.5-1cm, obtains sealwort particle;

B) sealwort particle is put into encloses container, vacuumize, then the container that sealwort particle is housed is put into heating installation and heated 30-60 second under 80-100 DEG C of environment;

C) the sealwort particle after heating is taken out, be cooled to normal temperature, then the sealwort particle after heating is sent into shredder, grind and be powder;

D) Solomonseal Rhizome is put into baking oven, setting-up time 30 seconds, 110 DEG C of temperature, toast;

E) in the purified water of 40-60 DEG C of the Solomonseal Rhizome input after baking, the 10-15 that adds water doubly measures and soaks 30 minutes, is 50-55 DEG C in temperature, acts on 5～10min, supersound extraction 3 times, merging filtrate under the condition that ultrasonic power is 440W/L;

F) filtrate is filtered, concentrate, add 95% ethanol of certain volume, leave standstill 24 hours, the supernatant liquor that inclines, decompress filter, 70 DEG C of vacuum-dryings, dry and obtain polygonatum polysaccharide.

The invention has the beneficial effects as follows: extraction time of the present invention is short, and energy consumption is low, medicinal raw material treatment capacity is large, at double or several times improve, and impurity is few, effective constituent is easy to separate, purify, cost is low, overall economic efficiency is remarkable.

Embodiment

For technique means, creation characteristic that the present invention is realized, reach object and effect is easy to understand, below in conjunction with specific embodiment, further set forth the present invention.

Embodiment 1:

A ultrasonic extraction process for polygonatum polysaccharide, comprises following methods step,

A) select the sealwort rhizome of water content in 15%, then sealwort rhizome is put into sifting machine, screening is clean, removes impurity, then puts into pulverizer and is ground into particulate state, and particle diameter, at 0.5-1cm, obtains sealwort particle;

B) sealwort particle is put into encloses container, vacuumize, then the container that sealwort particle is housed is put into heating installation and heated 30-60 second under 80-100 DEG C of environment;

C) the sealwort particle after heating is taken out, be cooled to normal temperature, then the sealwort particle after heating is sent into shredder, grind and be powder;

D) Solomonseal Rhizome is put into baking oven, setting-up time 30 seconds, 110 DEG C of temperature, toast;

E) in the purified water of 40-60 DEG C of the Solomonseal Rhizome input after baking, the 10-15 that adds water doubly measures and soaks 30 minutes, is 50-55 DEG C in temperature, acts on 5～10min, supersound extraction 3 times, merging filtrate under the condition that ultrasonic power is 440W/L;

F) filtrate is filtered, concentrate, add 95% ethanol of certain volume, leave standstill 24 hours, the supernatant liquor that inclines, decompress filter, 70 DEG C of vacuum-dryings, dry and obtain polygonatum polysaccharide.

More than show and described ultimate principle of the present invention and principal character and advantage of the present invention.The technician of the industry should understand; the present invention is not restricted to the described embodiments; that in above-described embodiment and specification sheets, describes just illustrates principle of the present invention; without departing from the spirit and scope of the present invention; the present invention also has various changes and modifications, and these changes and improvements all fall in the claimed scope of the invention.The claimed scope of the present invention is defined by appending claims and equivalent thereof.

Claims (1)

1. a ultrasonic extraction process for polygonatum polysaccharide, is characterized in that: comprise following methods step,

A) select the sealwort rhizome of water content in 15%, then sealwort rhizome is put into sifting machine, screening is clean, removes impurity, then puts into pulverizer and is ground into particulate state, and particle diameter, at 0.5-1cm, obtains sealwort particle;

B) sealwort particle is put into encloses container, vacuumize, then the container that sealwort particle is housed is put into heating installation and heated 30-60 second under 80-100 DEG C of environment;

C) the sealwort particle after heating is taken out, be cooled to normal temperature, then the sealwort particle after heating is sent into shredder, grind and be powder;

D) Solomonseal Rhizome is put into baking oven, setting-up time 30 seconds, 110 DEG C of temperature, toast;

E) in the purified water of 40-60 DEG C of the Solomonseal Rhizome input after baking, the 10-15 that adds water doubly measures and soaks 30 minutes, is 50-55 DEG C in temperature, acts on 5～10min, supersound extraction 3 times, merging filtrate under the condition that ultrasonic power is 440W/L;

F) filtrate is filtered, concentrate, add 95% ethanol of certain volume, leave standstill 24 hours, the supernatant liquor that inclines, decompress filter, 70 DEG C of vacuum-dryings, dry and obtain polygonatum polysaccharide.