CN105432620A - Bactericidal composition and application thereof - Google Patents

Bactericidal composition and application thereof Download PDF

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Publication number
CN105432620A
CN105432620A CN201510771957.0A CN201510771957A CN105432620A CN 105432620 A CN105432620 A CN 105432620A CN 201510771957 A CN201510771957 A CN 201510771957A CN 105432620 A CN105432620 A CN 105432620A
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agent
shen piperazine
piperazine mycin
bactericidal composition
bactericidal
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CN105432620B (en
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古越
李翔
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Sichuan Lier Crop Science Co Ltd
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Sichuan Lier Crop Science Co Ltd
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/48—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with two nitrogen atoms as the only ring hetero atoms
    • A01N43/60—1,4-Diazines; Hydrogenated 1,4-diazines
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/18—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing the group —CO—N<, e.g. carboxylic acid amides or imides; Thio analogues thereof
    • A01N37/22—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing the group —CO—N<, e.g. carboxylic acid amides or imides; Thio analogues thereof the nitrogen atom being directly attached to an aromatic ring system, e.g. anilides
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/18—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing the group —CO—N<, e.g. carboxylic acid amides or imides; Thio analogues thereof
    • A01N37/22—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing the group —CO—N<, e.g. carboxylic acid amides or imides; Thio analogues thereof the nitrogen atom being directly attached to an aromatic ring system, e.g. anilides
    • A01N37/24—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing the group —CO—N<, e.g. carboxylic acid amides or imides; Thio analogues thereof the nitrogen atom being directly attached to an aromatic ring system, e.g. anilides containing at least one oxygen or sulfur atom being directly attached to the same aromatic ring system
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
    • A01N43/40—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom six-membered rings

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  • Life Sciences & Earth Sciences (AREA)
  • Agronomy & Crop Science (AREA)
  • Pest Control & Pesticides (AREA)
  • Plant Pathology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Dentistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Environmental Sciences (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The invention discloses a bactericidal composition and the application thereof, and relates to the technical field of pesticide compounding. The bactericidal composition disclosed by the invention comprises the active ingredients namely phenazine-1-carboxylic acid A and succinate dehydrogenase inhibitor fungicides B, wherein the succinate dehydrogenase inhibitor fungicides B comprises one or more of fluopyram, dicyclic fluxapyroxad, penflufen or the like; the phenazine-1-carboxylic acid A and the succinate dehydrogenase inhibitor fungicides B are in the weight ratio of (1:100)-(100:1). The active ingredients A and B are compounded to achieve an obvious synergistic effect, and the application amount of each single preparation can be effectively reduced. The bactericidal composition has a good effect on enlarging the bacteriocidal spectrum and delaying the drug-resistance of plants and can also prolong the lasting period, so that the bactericidal composition has an obvious popularization value. The composition can be added with the auxiliary components which are allowed to be used in other pesticides, and can be processed into any preparation form.

Description

Bactericidal composition and application thereof
Technical field
The present invention relates to a kind of bactericidal composition and application thereof, refer to that a kind of active ingredient contains bactericidal composition and the application thereof of Shen piperazine mycin and succinate dehydrogenase inhibitors series bactericidal agent especially, belong to technical field of compounded pesticide.
Background technology
In agricultural production, can crops healthy growth, except affecting by worm, crop smothering, also very important to the control of disease.Along with reinforcement and the sustainable development implementation of environmental protection ideas, the inexorable trend efficient, low toxicity, high activity, low-residual have become Agrochemicals.But, even if efficient germicide, be used alone for a long time, also easily make disease develop immunity to drugs, cause the problems such as dosage increasing, preventive effect reduction, lasting period shortening, be unfavorable for environment sustainable development.Therefore, how Scientific Usage of Drugs, reduces the consumption of chemical pesticide, improves drug effect, becomes the problem that plant protection art is badly in need of solving.
Shen piperazine mycin, its structural formula is as shown in (I), Shen piperazine mycin is a kind of antibiotic secreted through biological culture by pseudomonas fluorescens M18, has wide spectrum Suppressing phytopathogens and the bactericide of the dual-use function of Promoting plant growth effect simultaneously, has wide spectrum, efficiently feature.
SDHI class (succinatedehydrogenaseinhibitors) bactericide is sterilized agent resistance Action Committee (FungicideResistanceActionCommittee, the FRAC) compound that newly a division class mechanism of action is out similar with resistance mechanism.The mechanism of action of this series bactericidal agent mainly suppresses pathogen SDA, thus disturbs its respiration, and its effect is special, strong drug action, persistent, obvious effect of increasing production.This series bactericidal agent that success has been developed at present has 15 kinds at least, and the major product released in recent years has biphenyl pyrrole bacterium amine (bixafen), fluorine pyrrole bacterium acid amides (fluopyram), fluorine azoles bacterium aniline (penflufen), dicyclo fluorine azoles bacterium amine (isopyrazam), ring benzene pyrrole bacterium amine (sedaxane), fluorine azoles bacterium acid amides (fluxapyroxad) and pyrrole metsulfovax (penthiopyrad).
Boscalid, English name: Cantus, boscalid, the chloro-N-of chemical name: 2-(4 '-chlorodiphenyl-2-base) vitamin PP, chemical molecular formula: C 18h 12cl 2n 2o, molecular weight: 343.21, CAS:188425-85-6.Boscalid is the Novel tobacco acid amide fungicides developed by BASF Aktiengesellschaft, be mainly used in preventing and treating powdery mildew, gray mold, various rot, brown rot and root rot etc., Boscalid belongs to succinic acid ubiquinone reductase inhibitor in mitochondrial respiratory chain, very strong rejection ability is had to the sprouting of spore, and with other bactericide without cross resistance.Boscalid is shifted in plant by blade face infiltration, suppresses mitochondrial succinate acid esters dehydrase, hinders tricarbonic acid cycle, and make amino acid, sugar lacks, energy reduces, the division of interference cell and growth, have neural activity to disease, has protection and therapeutic action.Suppress spore germination, the extension of bacterium pipe, mycelial growth and spore mother cell to form the Main Stage of conk and breeding, bactericidal action is directly caused by parent active agent, does not have corresponding metabolic activity.With carbendazim, Sukeling etc. without cross resistance.
Mebenil, English name: Mepronil, also known as: mebenil, mepronil, the third adjacent amine, line to reach gram etc., chemical name: N-(3-isopropyl phenyl)-2-methyl benzamide, molecular formula: C 17h 19nO 2, CAS:55814-41-0.Mebenil is a kind of systemic fungicide, can effectively prevent and treat various crop Major Diseases, and the term of validity is grown, is not easily produced poisoning, resistance of rainwater washing against, has special efficacy to by the fungus-caused crop pest of Basidiomycotina, germ can be stoped to invade host, play preventive and therapeutic action.Mebenil belongs to the succinate dehydrogenase thus the breathing of obstruction pathogen that suppress Complex II.Have and suppress and stop sheath blight fungus to invade host, reach preventive and therapeutic action, field control effectiveness test shows, has favorable effect to rice sheath blight disease.
Flutolanil, English name: Flutolanil, also known as: line cumidine, Wang Jiaduo, fluorine line amine, fluorine mepronil, chemical name: N-(3 '-isopropyl phenyl)-2-trifluoromethyl benzamide, molecular formula: C 17h 16f 3nO 2, molecular weight: 323.31, CAS:66332-96-5.Flutolanil belongs to heterocyclic carbamate derivatives, has the systemic fungicide of preventive and therapeutic action.Flutolanil belongs to succinate dehydrogenase inhibitors, suppresses the synthesis of aspartate and glutamate, and this medicament has protection and therapeutic action, can stop the growth of pathogen and penetrate, the microbial disease of cause of disease of main control Basidiomycota.To the fertility of mycelia and the formation of intrusion mycelia block, there is very strong inhibition.As prevented and treated the rice sheath blight disease that rhizoctonia causes, with the process of 50mg/L concentration cauline leaf, control efficiency 100%.
Banded sclerotial blight is also known as moire disease, and popular name spends sufficient stalk, rotten pin pest, features spot.Be infected by Rhizoctonia solani Kuhn to fall ill, how occur under high temperature, super-humid conditions.Banded sclerotial blight causes harm seriously in South Rice Region of China, is one of Major Diseases in current Rice Production.This disease makes paddy rice not ear, or the not plump paddy of heading is more, and grain heavily declines.
Powdery mildew all can be fallen ill to heading from seedling.Mainly to cause harm crop leaf, also cause harm stem and tassel.Blade starts produce yellow point, then expand and develop into circle or oval scab, the mould layer of the raw adularescent powdery in surface.Ordinary circumstance bottom blade is more than upper blade, and vacuum side of blade is more than front.Mildew is disperseed separately in early days, after be unified into a large mildew, even can cover full leaf, have a strong impact on photosynthesis, eubolism is interfered, causes early ageing, output incurs loss
Frequent use containing single-activity component bactericide, cause most germs to create pesticide resistance, preventive effect is unsatisfactory.Therefore, be badly in need of the sterilised products that a kind of novel product substitutes single-activity component, increase the resistance risk that drug effect delays disease, reduce economic loss.
Summary of the invention
It is reasonable that the technical problem to be solved in the present invention is to provide a kind of component, and synergistic effect is remarkable, and bactericidal effect is good, and drug cost is low, not easily develops immunity to drugs, to the bactericidal composition of crop safety and application thereof.
For solving the problems of the technologies described above, the invention provides technical scheme as follows:
Bactericidal composition of the present invention, containing effective constituents A and active ingredient B, effective constituents A is Shen piperazine mycin, active ingredient B is succinate dehydrogenase inhibitors series bactericidal agent, the weight ratio of effective constituents A Shen piperazine mycin and active ingredient B succinate dehydrogenase inhibitors series bactericidal agent is 1:100 ~ 100:1, being preferably weight ratio is 1:50 ~ 50:1, and more preferably weight ratio is 1:25 ~ 25:1.
In bactericidal composition of the present invention, described active ingredient B succinate dehydrogenase inhibitors series bactericidal agent can be one or more of Boscalid, mebenil or flutolanil.
Pesticidal preparations auxiliary element can also be contained, to make the formulation being applicable to agriculturally using in bactericidal composition of the present invention.Described pesticidal preparations auxiliary element is one or more in solvent, emulsifier, dispersant, stabilizing agent, antifreezing agent or wetting agent.
Bactericidal composition of the present invention can be prepared into any one formulation of applicable agricultural use by known method, reasonable formulation is dry suspending agent (DF), wetting powder (WP), water dispersible granules (WDG), suspending agent (SC), oil-suspending agent, solvable pulvis, granule, microemulsion, aqueous emulsion, microcapsule suspending agent etc.
A wetting powder (WP) is can homodisperse solid powder preparation in water.Wetting powder of the present invention except adding as white carbon in former medicine, precipitated calcium carbonate, potter's clay, diatomite, outside the thinners such as attapulgite or inert substance, also have ionic or nonionic surface active agent (wetting agent, dispersant), wetting agent such as, alkylbenzenesulfonate (DBS-Na), alkylnaphthalene sulfonate (pulling open powder), alkylphenol-polyethenoxy base ether sulfate, alkylphenol-polyethenoxy base ether formaldehyde condensation products sulphate, aliphatic alcohol sulfate, fatty alcohol ethylene oxide adduct sulfonate, alkyl amide taurate, aliphatic alcohol polyethenoxy base ether (JFC), content 0.1 ~ 30%, dispersant is as naphthalene sulfonate, naphthalene or alkyl naphthalene formaldehyde condensate sulfonates (NO, NN0, MF), lignin and derivative sulfonate (M-9, POLYFON etc.) thereof, polyoxyethylene poly-oxygen propylene aether block copolymer, alkylphenol-polyethenoxy base ether formaldehyde condensation products sulphate (SOPA), PVA, CMC content 0.1 ~ 30%, also containing one or more materials among penetrating agent JFC, azone, thiophene ketone, penetrant t, content 0.3 ~ 30%, other auxiliary agents such as stabilizing agent, foam inhibitor, anticaking agent can also be contained.
B water dispersible granules (WDG) very fast disintegration in water, dispersion can form the granule of higher suspension dispersion.By active component, the key element such as auxiliary agent and the carrier compositions such as wetting agent, dispersant, interleaving agent, disintegrant, stabilizing agent, adhesive.Its auxiliary agent and carrier, and the compound method of precursor (preformed objects before granulation) is analogous to wetting powder and suspending agent substantially.
C suspending agent (SC) is not water soluble solid agricultural chemicals or the dispersion of immiscible liquids agricultural chemicals in water or oil.Obtained through soybean dietary fiber by active component, auxiliary agent (wetting dispersing agent, thickener, stabilizing agent, pH adjusting agent, defoamer, antifreezing agent).Wetting dispersing agent can be naphthalene or alkyl naphthalene formaldehyde condensate sulfonates (NO, NN0, MF), lignin and derivative sulfonate (M-9, POLYFON etc.) thereof, alkylphenol-polyethenoxy base ether sulfonate, polyoxyethylene polyoxypropylene base ether block copolymers, one or more in alkylphenol-polyethenoxy base phosphate, carboxymethyl cellulose (CMC), polyvinylpyrrolidone (PVP), polyethylene glycol (PEG), condensed phosphate etc., content 0.1 ~ 12%; Thickener and stabilizing agent can be one or more in xanthan gum (XG), polyvinyl alcohol (PVA), Magnesiumaluminumsilicate etc., content 0.05 ~ 0.5%; PH adjusting agent and defoamer can optionally add in right amount.
D dry suspending agent (DryFlowable, be called for short DF), be can very fast disintegration in water, dispersion formation higher suspension dispersion granule.Preparation method: the preparation obtained by the dehydration of suspending agent.Its auxiliary agent and carrier, and the compound method of precursor (preformed objects before dehydrating) is consistent with suspending agent.
Bactericidal composition of the present invention is applicable to the pesticidal preparations preparing controlling plant diseases.Be particularly useful for making the pesticidal preparations of the water prevention rice sheath blight, rice blast, false smut, wheat rust, powdery mildew, downy mildew, gray mold, epidemic disease, scab, spot defoliation, net blotch, glume blight, eyeprint disease, ring spot, fusarium wilt, gray mold, anthracnose, canker, banded sclerotial blight, stalk break.
Bactericidal composition of the present invention can finished product preparation form provide, namely in bactericidal composition, each component substances mixes, or the composition of bactericidal composition also can provide with unit dose form, directly directly mix in bucket or tank before using, be then diluted to required concentration.
Bactericidal composition of the present invention is applied to the method for controlling disease, and effective constituents A and composition B use with above-mentioned weight ratio, and it is used after can mixing in proportion, use in proportion simultaneously or use successively in proportion.Concrete method of application can be powder injection process, spray-on process, smog method, fumigating system and seed treatment and soil treatment, ultra low volume spraying, mixes fertilizer, spreads fertilizer over the fields, fills with root.
Compared with prior art, beneficial effect of the present invention is:
1, compared with single dose, composite have obvious synergistic function, improves control efficiency;
2, composition is made up of the active ingredient that two kinds of mechanism of action are different, and bactericidal effect is good, is conducive to overcoming and delay the drug-fast generation of germ;
3, medicament mixture decreases dosage, thus reduces cost and alleviate the pollution to environment.
Instant component is reasonable, and treatment adds protective effect, and drug cost is low, and its activity and bactericidal effect are not the simple superposition of each composition activity, but has significant synergistic effect, good to crop safety, meets the security requirement of pesticidal preparations.
Embodiment
For making the technical problem to be solved in the present invention, technical scheme and advantage clearly, will be described in detail with specific embodiment below, but the present invention is limited to absolutely not these examples.The following stated is only the good embodiment of the present invention, only in order to explain the present invention, therefore can not be interpreted as the restriction to the scope of the claims of the present invention.It should be pointed out that all any amendments done within the spirit and principles in the present invention, equivalent replacement and improvement etc., all should be included within protection scope of the present invention.Therefore, the protection domain of patent of the present invention should be as the criterion with claims.
The combination of active principles of different agricultural chemicals is made Recompounded pesticide, is current development novel pesticide and the one of preventing and treating agriculturally resistance germ effectively and efficiently mode.After the pestsides synthesis of different cultivars, usually show a large amount of three kinds of type of action: summation action, synergistic effect and antagonism.Compound synergic is well filled a prescription, and can significantly improve actual control efficiency, and reducing the usage amount of agricultural chemicals, thus contribute to delaying the drug-fast generation speed of germ, is the important means of integrated control disease.
The present composition with Shen piperazine mycin for a kind of active ingredient (A), succinate dehydrogenase inhibitors series bactericidal agent is another active ingredient (B), combine between them, to plant disease, there is obvious synergistic function, and be not only the simple superposition of two kinds of pharmacy effects.
In order to prevent and treat the gray mold of cucumber in agricultural production, inventor carries out composite synergy research with Shen piperazine mycin and Boscalid, and concrete grammar is:
Test adopts gray mold of cucumber to be tested object.
Former medicine is mixed with the test medicine of needs, test method: fungicide bioassay pattern--cotyledon method, with reference to " People's Republic of China's agricultural industry criteria ".Choose broad bean susceptible variety potted plant, clip same section, growing way is consistent, be with petiolate blade, put in culture dish, moisturizing is for subsequent use, experimental pharmacy is adopted former medicinal water dissolved dilution, 5 mass concentrations are set, blade is fully infiltrated 5S in pre-configured good liquid, drains unnecessary liquid, after natural air drying, cultivate by moisturizing after marks for treatment, test set not containing the process of medicament as blank.Be that 5mm bacterium cake has the one side of mycelia to be inoculated in process central vane with inoculator by diameter, 30 leaves are inoculated in each process.Protectiveness test is 24h inoculation after chemicals treatment, and curative tests is 24h inoculation before chemicals treatment.Inoculation is placed on climatic cabinate or constant temperature has in illumination humidistat in fact, cultivates under the condition of temperature 26 degrees Celsius-28 degrees Celsius, relative moisture 80%-90%.According to blank incidence, with kind of calliper record each vaccination scab length and width, with length, wide mean value represents lesion diameter record lesion diameter (unit mm). according to survey data, by preventing and treating result publicity: preventive effect=(blank diameter-process diameter)/blank diameter * 100, calculate the percentage that each process suppresses lesions grow, by the linear regression analysis between the probit value of inhibiting rate and the logarithm value of series concentration, obtain the EC50 value of each medicament, the co-toxicity coefficient (CTC) of mixture is calculated with co-toxicity coefficient table, the activity of reagent agent to germ is evaluated with this.
The co-toxicity coefficient (CTC) >=120 of complex preparation shows as synergistic effect; CTC≤80 show as antagonism; 80 ﹤ CTC ﹤ 120 show as summation action.
Table 1 Shen piperazine mycin and the composite Toxicity Determination to gray mold of cucumber of Boscalid
Chemicals treatment Proportioning Virulence regression equation EC50(mg/L) Co-toxicity coefficient (CTC)
Shen piperazine mycin (A) —— Y＝4.8588+0.3241X 2.7261
Boscalid (B1) —— Y＝3.8338+1.0540X 12.7753
A:B1 1:100 Y＝4.2665+0.8040X 8.1689 150.88
A:B1 1:50 Y＝4.3814+0.7464X 6.7383 177.18
A:B1 1:25 Y＝4.5337+0.6124X 5.7710 193.91
A:B1 1:1 Y＝4.6515+1.0223X 2.1921 204.98
A:B1 25:1 Y＝4.6810+1.2596X 1.7913 156.94
A:B1 50:1 Y＝4.6582+1.2264X 1.8994 145.786
A:B1 100:1 Y＝4.5830+1.2780X 2.1194 129.64
Toxicity Determination result shows, within the scope of weight ratio 1:100-100:1, particularly within the scope of 1:50-50:1, especially within the scope of 1:25-25:1, Shen piperazine mycin and Boscalid is composite has obvious synergistic effect to gray mold of cucumber, control efficiency is excellent and be better than single dose kind.
In order to prevent and treat the rice sheath blight disease in agricultural production, inventor carries out composite synergy research with Shen piperazine mycin and mebenil, and concrete grammar is:
Test adopts rice sheath blight disease to be tested object.
Former medicine is mixed with the test medicine of needs, test method (in vitro culture method) is with reference to " People's Republic of China's agricultural industry criteria ".First single dose and each Mixed Pharmacy are arranged 5 variable concentrations gradients (on the basis of preliminary experiment result, different with proportioning according to different agents combination, concentration arranges also different, and bacteriostasis rate sets in the scope of 5% ~ 95%).Medicament is fully infiltrated 5s in the liquid prepared in advance, drains unnecessary liquid, after natural air drying, each process blade and blade is gone up dorsad, for subsequent use by being emitted on after marks for treatment in moisture preservation box, test set not containing the process of medicament as blank.The one side of mycelia there is is to be inoculated in rice leaf central authorities the sheath blight fungus prepared with inoculator, often process inoculation 30 leaves, protectiveness test is 24h inoculation after chemicals treatment, curative tests is 24h inoculation before chemicals treatment, it is 26 ~ 28 DEG C that inoculation is placed on temperature, and humidity is cultivate in the climatic cabinate of 80% ~ 90%; According to blank incidence, record lesion diameter, calculate the percentage that each process suppresses lesions grow, by the linear regression analysis between the probit value of inhibiting rate and the logarithm value of series concentration, obtain the EC50 value of each medicament, calculate the co-toxicity coefficient (CTC) of mixture with co-toxicity coefficient table, evaluate the activity of reagent agent to germ with this.The co-toxicity coefficient (CTC) >=120 of complex preparation shows as synergistic effect; CTC≤80 show as antagonism; 80 ﹤ CTC ﹤ 120 show as summation action.
Table 2 Shen piperazine mycin and the composite Toxicity Determination to rice sheath blight disease of mebenil
Chemicals treatment Proportioning Virulence regression equation EC50(mg/L) Co-toxicity coefficient (CTC)
Shen piperazine mycin (A) —— Y＝4.3775+0.6081X 10.5548 ——
Mebenil (B2) —— Y＝3.5893+0.9992X 25.8087 ——
A:B2 1:100 Y＝3.8688+0.8970X 18.2398 139.50
A:B2 1:50 Y＝3.8864+0.9541X 14.6907 170.84
A:B2 1:25 Y＝3.8458+1.0379X 12.9395 188.96
A:B2 1:1 Y＝3.7448+1.5318X 6.5976 227.08
A:B2 25:1 Y＝3.6606+1.5900X 6.9557 155.27
A:B2 50:1 Y＝3.7041+1.5011X 7.2987 147.97
A:B2 100:1 Y＝3.7977+1.3299X 8.0169 132.44
Inventor finds through test, within the scope of weight ratio 1:100-100:1, particularly within the scope of 1:50-50:1, especially within the scope of 1:25-25:1, Shen piperazine mycin and mebenil composite, to the effect that rice sheath blight disease has obvious synergistic effect similar, there is obvious Complex synergism to plant disease.
In order to prevent and treat the powdery mildew of cucumber in agricultural production, inventor carries out composite synergy research with Shen piperazine mycin and flutolanil, and concrete grammar is:
Test adopts powdery mildew of cucumber to be tested object.
Former medicine is mixed with the test medicine of needs, test method (pot-culture method) is with reference to " People's Republic of China's agricultural industry criteria ".First single dose and each Mixed Pharmacy are arranged 5 variable concentrations gradients (on the basis of preliminary experiment result, different with proportioning according to different agents combination, concentration arranges also different, and bacteriostasis rate sets in the scope of 5% ~ 95%).With spray-on process by medicine liquid spray on wheat seedling for subsequent use, naturally dry rear for subsequent use, test set not containing the process of medicament as blank.The fresh sporozoite of Powdery Mildew produced in 24h on morbidity wheat leaf blade evenly is tiltedly fallen to being inoculated on the 2-3 leaf phase potted plant wheat seedling of process, often processes 3 basins.Protectiveness test 24h after chemicals treatment, curative tests is 24h inoculation before chemicals treatment, then puts 28 degrees Celsius of constant temperature lighting box and cultivates.According to blank incidence, record lesion diameter, calculate the percentage that each process suppresses lesions grow, by the linear regression analysis between the probit value of inhibiting rate and the logarithm value of series concentration, obtain the EC50 value of each medicament, calculate the co-toxicity coefficient (CTC) of mixture with co-toxicity coefficient table, evaluate the activity of reagent agent to germ with this.The co-toxicity coefficient (CTC) >=120 of complex preparation shows as synergistic effect; CTC≤80 show as antagonism; 80 ﹤ CTC ﹤ 120 show as summation action.
Table 3 Shen piperazine mycin and the composite Toxicity Determination to powdery mildew of cucumber of flutolanil
Chemicals treatment Proportioning Virulence regression equation EC50(mg/L) Co-toxicity coefficient (CTC)
Shen piperazine mycin (A) —— Y＝3.5870+0.9381X 32.0653 ——
Flutolanil (B3) —— Y＝3.5893+0.9992X 25.8087 ——
A:B3 1:100 Y＝3.8754+0.8749X 19.2868 134.07
A:B3 1:50 Y＝3.9176+0.9388X 14.2183 182.21
A:B3 1:25 Y＝3.9476+0.9325X 13.4435 193.42
A:B3 1:1 Y＝3.9632+0.9258X 13.1762 217.04
A:B3 25:1 Y＝3.8590+0.9353X 16.5893 191.50
A:B3 50:1 Y＝3.7756+0.9132X 21.9102 145.65
A:B3 100:1 Y＝3.7386+0.9197X 23.5185 136.01
Toxicity Determination result shows, within the scope of weight ratio 1:100-100:1, particularly within the scope of 1:50-50:1, especially within the scope of 1:25-25:1, Shen piperazine mycin and flutolanil is composite has obvious synergistic effect to powdery mildew of cucumber, control efficiency is excellent and be better than single dose kind.
Bactericidal composition of the present invention can be prepared into any one formulation of applicable agricultural use by known method, reasonable formulation is wetting powder, aqueous suspension agent, oil-suspending agent, suspended emulsion, water dispersible granules.In all formulas, percentage is all weight percentage.The processing technology of the various formulation of the present composition is prior art, can change to some extent according to different situations.
For making the technical problem to be solved in the present invention, technical scheme and advantage clearly, within the scope of weight ratio 1:100 ~ 100:1, build the embodiment that Shen piperazine mycin is composite with Boscalid, mebenil or flutolanil respectively, be described in detail, but the present invention is limited to absolutely not these examples.
In addition, inventor also found through experiments, Shen piperazine mycin respectively with the composite effect of Boscalid, mebenil or flutolanil, be better than the composite effect of Shen piperazine mycin and other succinate dehydrogenase inhibitors series bactericidal agents.In order to this problem is clearly described, within the scope of weight ratio 1:100 ~ 100:1, build the comparative example of Shen piperazine mycin and other succinate dehydrogenase inhibitors series bactericidal agents, be described in detail.But because length is limited, representative several comparative examples can only be enumerated.It should be pointed out that Shen piperazine mycin respectively with the composite effect of Boscalid, mebenil or flutolanil, be better than the composite effect of Shen piperazine mycin and other succinate dehydrogenase inhibitors series bactericidal agents, be not only confined in limited comparative example.
Below in conjunction with embodiment, comparative example, content of the present invention is described further.Embodiment comparative example
One: Shen piperazine mycin and Boscalid
Embodiment 1:30.3% Shen piperazine mycin Boscalid suspending agent (1:100)
Component: Shen piperazine mycin 0.3%, Boscalid 30%, acrylic acid-acrylic acid hydroxypropyl acrylate copolymer 5%, fatty alcohol-polyoxyethylene ether 3%, ethylene glycol 6%, XG0.3%, silicone defoaming agent 0.2%, Sodium Benzoate 0.6%, soft water complements to 100%.
Preparation method: each component is mixed, mixes through high speed shear, then by sand mill sand milling 2-3 hour, make average grain diameter reach 1-5 micron, 30.3% Shen piperazine mycin Boscalid suspending agent can be obtained.
Comparative example 1:30.3% Shen piperazine mycin thifluzamide suspending agent (1:100)
Component: Shen piperazine mycin 0.3%, thifluzamide 30%, acrylic acid-acrylic acid hydroxypropyl acrylate copolymer 5%, fatty alcohol-polyoxyethylene ether 3%, ethylene glycol 6%, XG0.3%, silicone defoaming agent 0.2%, Sodium Benzoate 0.6%, soft water complements to 100%.
Preparation method: the same.
Embodiment 2:33.8% Shen piperazine mycin Boscalid water dispersible granules (1:83.5)
Component: Shen piperazine mycin 0.4%, Boscalid 33.4%, polycarboxylic acids sodium 3%, sodium lignin sulfonate 6%, anhydrous sodium sulfate 8%, kaolin complements to 100%.
Preparation method: each component mixed, through mechanical crushing, then through air-flow crushing, mixes, then dries through wet granulation, can obtain 33.8% Shen piperazine mycin Boscalid water dispersible granules.
Comparative example 2:33.8% jinggangmeisu Boscalid water dispersible granules (1:83.5)
Component: jinggangmeisu 0.4%, Boscalid 33.4%, polycarboxylic acids sodium 3%, sodium lignin sulfonate 6%, anhydrous sodium sulfate 8%, kaolin complements to 100%.
Preparation method: the same.
Embodiment 3:30.6% Shen piperazine mycin Boscalid dry suspending agent (1:50)
Component: Shen piperazine mycin 0.6%, Boscalid 30%, polycarboxylic acids sodium 4%, sodium lignin sulfonate 8%, white carbon 8%, silicone defoaming agent 0.4%, Sodium Benzoate 0.1%, kaolin complements to 100%.Soft water 150-200%.
Preparation method: the water adding 1.5-2.0 times of Formulation amounts, each component is mixed, mixes through high speed shear, then by sand mill sand milling 2-3 hour, make average grain diameter reach 1-5 micron, dehydrate and can obtain 30.6% Shen piperazine mycin Boscalid dry suspending agent.
Comparative example 3:30.6% Shen piperazine mycin thifluzamide dry suspending agent (1:50)
Component: Shen piperazine mycin 0.6%, thifluzamide 30%, polycarboxylic acids sodium 4%, sodium lignin sulfonate 8%, white carbon 8%, silicone defoaming agent 0.4%, Sodium Benzoate 0.1%, kaolin complements to 100%.Soft water 150-200%.
Preparation method: the same.
Embodiment 4:35% Shen piperazine mycin Boscalid dry suspending agent (1:34)
Component: Shen piperazine mycin 1%, Boscalid 34%, lignin sodium naphthalene sulfonate 8%, sodium lignin sulfonate 8%, white carbon 8%, silicone defoaming agent 0.4%, Sodium Benzoate 0.1%, talcum powder complements to 100%.Soft water 150-200%.
Preparation method: the water adding 1.5-2.0 times of Formulation amounts, each component is mixed, mixes through high speed shear, then by sand mill sand milling 2-3 hour, make average grain diameter reach 1-5 micron, dehydrate and can obtain 34% Shen piperazine mycin Boscalid dry suspending agent.
Comparative example 4:35% polyoxin Boscalid dry suspending agent (1:34)
Component: polyoxin 1%, Boscalid 34%, lignin sodium naphthalene sulfonate 8%, sodium lignin sulfonate 8%, white carbon 8%, silicone defoaming agent 0.4%, Sodium Benzoate 0.1%, talcum powder complements to 100%.Soft water 150-200%.
Preparation method: the same.
Embodiment 5:26% Shen piperazine mycin Boscalid water dispersible granules (1:25)
Component: Shen piperazine mycin 1%, Boscalid 25%, polycarboxylic acids sodium 3%, sodium naphthalene sulfonate 5%, soluble starch 6%, kaolin complements to 100%.
Preparation method: each component mixed, through mechanical crushing, then through air-flow crushing, mixes, then dries through wet granulation, can obtain 26% Shen piperazine mycin Boscalid water dispersible granules.
Comparative example 5:26% Shen piperazine mycin thifluzamide water dispersible granule (1:25)
Component: Shen piperazine mycin 1%, thifluzamide 25%, polycarboxylic acids sodium 3%, sodium naphthalene sulfonate 5%, soluble starch 6%, kaolin complements to 100%.
Preparation method: the same.
Embodiment 6:20% Shen piperazine mycin Boscalid dry suspending agent (1:1)
Component: Shen piperazine mycin 10%, Boscalid 10%, lignin sodium naphthalene sulfonate 6%, sodium lignin sulfonate 6%, white carbon 6%, silicone defoaming agent 0.4%, Sodium Benzoate 0.1%, talcum powder complements to 100%.Soft water 150-200%.
Preparation method: the water adding 1.5-2.0 times of Formulation amounts, each component is mixed, mixes through high speed shear, then by sand mill sand milling 2-3 hour, make average grain diameter reach 1-5 micron, dehydrate and can obtain 20% Shen piperazine mycin Boscalid dry suspending agent.
Comparative example 6:20% Shen piperazine mycin thifluzamide dry suspending agent (1:1)
Component: Shen piperazine mycin 10%, thifluzamide 10%, lignin sodium naphthalene sulfonate 6%, sodium lignin sulfonate 6%, white carbon 6%, silicone defoaming agent 0.4%, Sodium Benzoate 0.1%, talcum powder complements to 100%.Soft water 150-200%.
Preparation method: the same.
Comparative example 7:20% kasugarnycin Boscalid dry suspending agent (1:1)
Component: kasugarnycin 10%, Boscalid 10%, lignin sodium naphthalene sulfonate 6%, sodium lignin sulfonate 6%, white carbon 6%, silicone defoaming agent 0.4%, Sodium Benzoate 0.1%, talcum powder complements to 100%.Soft water 150-200%.
Preparation method: the same.
Embodiment 7:26% Shen piperazine mycin Boscalid suspending agent (25:1)
Component: Shen piperazine mycin 25%, Boscalid 1%, polycarboxylic acids sodium 2%, fatty alcohol-polyoxyethylene ether 4%, 1,2-PD 5%, XG0.33%, silicone defoaming agent 0.2%, Sodium Benzoate 0.6%, soft water complements to 100%.
Preparation method: each component is mixed, mixes through high speed shear, then by sand mill sand milling 2-3 hour, make average grain diameter reach 1-5 micron, 26% Shen piperazine mycin Boscalid suspending agent can be obtained.
Embodiment 8:25.6% Shen piperazine mycin Boscalid wetting powder (31:1)
Component: Shen piperazine mycin 24.8%, Boscalid 0.8%, lauryl sodium sulfate 4%, sodium naphthalene sulfonate 6%, white carbon 10%, precipitated calcium carbonate complements to 100%.
Preparation method: each component mixed, first through mechanical crushing, then after air-flow crushing, mixes, and can obtain 25.6% Shen piperazine mycin Boscalid wetting powder.
Comparative example 8:25.6% polyoxin Boscalid wetting powder (31:1)
Component: polyoxin 24.8%, Boscalid 0.8%, lauryl sodium sulfate 4%, sodium naphthalene sulfonate 6%, white carbon 10%, precipitated calcium carbonate complements to 100%.
Preparation method: the same.
Embodiment 9:32.4% Shen piperazine mycin Boscalid suspending agent (80:1)
Component: Shen piperazine mycin 32%, Boscalid 0.4%, polycarboxylic acids sodium 2%, OPEO 4%, ethylene glycol 6%, XG0.3%, silicone defoaming agent 0.2%, Sodium Benzoate 0.6%, soft water complements to 100%.
Preparation method: each component is mixed, mixes through high speed shear, then by sand mill sand milling 2-3 hour, make average grain diameter reach 1-5 micron, 32.4% Shen piperazine mycin Boscalid suspending agent can be obtained.
Comparative example 9:32.4% Shen piperazine mycin thifluzamide suspending agent (80:1)
Component: Shen piperazine mycin 32%, thifluzamide 0.4%, polycarboxylic acids sodium 2%, OPEO 4%, ethylene glycol 6%, XG0.3%, silicone defoaming agent 0.2%, Sodium Benzoate 0.6%, soft water complements to 100%.
Preparation method: the same.
Two, Shen piperazine mycin and mebenil
Embodiment 10:40.4% Shen piperazine mycin mebenil suspending agent (1:100)
Component: Shen piperazine mycin 0.4%, mebenil 40%, acrylic acid-acrylic acid hydroxypropyl acrylate copolymer 5%, OPEO sodium sulfonate 3%, 1,2-PD 5%, XG0.25%, silicone defoaming agent 0.2%, Sodium Benzoate 0.4%, soft water complements to 100%.
Preparation method: each component is mixed, mixes through high speed shear, then by sand mill sand milling 2-3 hour, make average grain diameter reach 1-5 micron, 40.4% Shen piperazine mycin mebenil suspending agent can be obtained.
Comparative example 10:40.4% tetramycin mebenil suspending agent (1:100)
Component: tetramycin 0.4%, mebenil 40%, acrylic acid-acrylic acid hydroxypropyl acrylate copolymer 5%, OPEO sodium sulfonate 3%, 1,2-PD 5%, XG0.25%, silicone defoaming agent 0.2%, Sodium Benzoate 0.4%, soft water complements to 100%.
Preparation method: the same.
Embodiment 11:33.3% Shen piperazine mycin mebenil wetting powder (1:65.6)
Component: Shen piperazine mycin 0.5%, mebenil 32.8%, neopelex 3%, sodium lignin sulfonate formaldehyde condensation products 6%, white carbon 6%, precipitated calcium carbonate complements to 100%.
Preparation method: each component mixed, first through mechanical crushing, then after air-flow crushing, mixes, and can obtain 33.3% Shen piperazine mycin mebenil wetting powder.
Comparative example 11:33.3% Shen piperazine mycin thifluzamide wetting powder (1:65.6)
Component: Shen piperazine mycin 0.5%, thifluzamide 32.8%, neopelex 3%, sodium lignin sulfonate formaldehyde condensation products 6%, white carbon 6%, precipitated calcium carbonate complements to 100%.
Preparation method: the same.
Embodiment 12:36% Shen piperazine mycin mebenil wetting powder (1:35)
Component: Shen piperazine mycin 1%, mebenil 35%, neopelex 4%, sodium naphthalene sulfonate 8%, white carbon 8%, kaolin complements to 100%.
Preparation method: each component mixed, first through mechanical crushing, then after air-flow crushing, mixes, and can obtain 36% Shen piperazine mycin mebenil wetting powder.
Comparative example 12:36% Shen piperazine mycin thifluzamide wetting powder (1:35)
Component: Shen piperazine mycin 1%, thifluzamide 35%, neopelex 4%, sodium naphthalene sulfonate 8%, white carbon 8%, kaolin complements to 100%.
Preparation method: the same.
Embodiment 13:26% Shen piperazine mycin mebenil suspending agent (25:1)
Component: Shen piperazine mycin 25%, mebenil 1%, polycarboxylic acids sodium 3%, fatty alcohol-polyoxyethylene ether 3%, ethylene glycol 6%, XG0.3%, silicone defoaming agent 0.2%, Sodium Benzoate 0.6%, soft water complements to 100%.
Preparation method: each component is mixed, mixes through high speed shear, then by sand mill sand milling 2-3 hour, make average grain diameter reach 1-5 micron, 26% Shen piperazine mycin mebenil suspending agent can be obtained.
Comparative example 13:26% Shen piperazine mycin thifluzamide suspending agent (25:1)
Component: Shen piperazine mycin 25%, thifluzamide 1%, polycarboxylic acids sodium 3%, fatty alcohol-polyoxyethylene ether 3%, ethylene glycol 6%, XG0.3%, silicone defoaming agent 0.2%, Sodium Benzoate 0.6%, soft water complements to 100%.
Preparation method: the same.
Embodiment 14:25.5% Shen piperazine mycin mebenil water dispersible granules (50:1)
Component: Shen piperazine mycin 25%, mebenil 0.5%, lignin sodium naphthalene sulfonate 3%, polycarboxylic acids sodium 4%, anhydrous sodium sulfate 8%, kaolin complements to 100%.
Preparation method: each component mixed, through mechanical crushing, then through air-flow crushing, mixes, then dries through wet granulation, can obtain 25.5% Shen piperazine mycin mebenil water dispersible granules.
Comparative example 14:25.5% tetramycin mebenil water dispersible granules (50:1)
Component: tetramycin 25%, mebenil 0.5%, lignin sodium naphthalene sulfonate 3%, polycarboxylic acids sodium 4%, anhydrous sodium sulfate 8%, kaolin complements to 100%.
Preparation method: the same
Embodiment 15:20.2% Shen piperazine mycin mebenil dry suspending agent (100:1)
Component: Shen piperazine mycin 20%, mebenil 0.2%, lignin sodium naphthalene sulfonate 6%, sodium lignin sulfonate 6%, white carbon 10%, silicone defoaming agent 0.4%, Sodium Benzoate 0.1%, kaolin complements to 100%.Soft water 150-200%.
Preparation method: the water adding 1.5-2.0 times of Formulation amounts, each component is mixed, mixes through high speed shear, then by sand mill sand milling 2-3 hour, make average grain diameter reach 1-5 micron, dehydrate and can obtain 20.2% Shen piperazine mycin mebenil dry suspending agent.
Comparative example 15:20.2% Shen piperazine mycin thifluzamide microcapsule suspending agent (100:1)
Component: Shen piperazine mycin 20%, thifluzamide 0.2%, lignin sodium naphthalene sulfonate 6%, sodium lignin sulfonate 6%, white carbon 10%, silicone defoaming agent 0.4%, Sodium Benzoate 0.1%, kaolin complements to 100%.Soft water 150-200%.
Preparation method: the same.
Three, Shen piperazine mycin and flutolanil
Embodiment 16:40.4% Shen piperazine mycin flutolanil suspending agent (1:100)
Component: Shen piperazine mycin 0.4%, flutolanil 40%, polycarboxylic acids sodium 4%, OPEO phosphate 2%, ethylene glycol 6%, XG0.25%, silicone defoaming agent 0.2%, Sodium Benzoate 0.6%, soft water complements to 100%.
Preparation method: each component is mixed, mixes through high speed shear, then by sand mill sand milling 2-3 hour, make average grain diameter reach 1-5 micron, 40.4% Shen piperazine mycin flutolanil suspending agent can be obtained.
Comparative example 16:40.4% polyoxin flutolanil suspending agent (1:100)
Component: polyoxin 0.4%, flutolanil 40%, polycarboxylic acids sodium 4%, OPEO phosphate 2%, ethylene glycol 6%, XG0.25%, silicone defoaming agent 0.2%, Sodium Benzoate 0.6%, soft water complements to 100%.
Preparation method: the same.
Embodiment 17:46% Shen piperazine mycin flutolanil wetting powder (1:45)
Component: Shen piperazine mycin 1%, flutolanil 45%, neopelex 4%, lignin sodium naphthalene sulfonate 8%, white carbon 8%, precipitated calcium carbonate complements to 100%.
Preparation method: each component mixed, first through mechanical crushing, then after air-flow crushing, mixes, and can obtain 46% Shen piperazine mycin flutolanil wetting powder.
Comparative example 17:46% Shen piperazine mycin thifluzamide wetting powder (1:45)
Component: Shen piperazine mycin 1%, thifluzamide 45%, neopelex 4%, lignin sodium naphthalene sulfonate 8%, white carbon 8%, precipitated calcium carbonate complements to 100%.
Preparation method: the same.
Embodiment 18:20% Shen piperazine mycin flutolanil dry suspending agent (1:1)
Component: Shen piperazine mycin 10%, flutolanil 10%, lignin sodium naphthalene sulfonate 6%, sodium lignin sulfonate 6%, white carbon 8%, silicone defoaming agent 0.2%, Sodium Benzoate 0.1%, kaolin complements to 100%.Soft water 150-200%.
Preparation method: the water adding 1.5-2.0 times of Formulation amounts, each component is mixed, mixes through high speed shear, then by sand mill sand milling 2-3 hour, make average grain diameter reach 1-5 micron, dehydrate and can obtain 20% Shen piperazine mycin flutolanil dry suspending agent.
Comparative example 18:20% jinggangmeisu flutolanil dry suspending agent (1:1)
Component: jinggangmeisu 10%, flutolanil 10%, lignin sodium naphthalene sulfonate 6%, sodium lignin sulfonate 6%, white carbon 8%, silicone defoaming agent 0.2%, Sodium Benzoate 0.1%, kaolin complements to 100%.Soft water 150-200%.
Preparation method: the same.
Embodiment 19:25.5% Shen piperazine mycin flutolanil water dispersible granules (50:1)
Component: Shen piperazine mycin 25%, flutolanil 0.5%, lignin sodium naphthalene sulfonate 6%, polycarboxylic acids sodium 4%, anhydrous sodium sulfate 8%, kaolin complements to 100%.
Preparation method: each component mixed, through mechanical crushing, then through air-flow crushing, mixes, then dries through wet granulation, can obtain 25.5% Shen piperazine mycin flutolanil water dispersible granules.
Comparative example 19:25.5% Shen piperazine mycin thifluzamide water dispersible granule (50:1)
Component: Shen piperazine mycin 25%, thifluzamide 0.5%, lignin sodium naphthalene sulfonate 6%, polycarboxylic acids sodium 4%, anhydrous sodium sulfate 8%, kaolin complements to 100%.
Preparation method: the same.
Embodiment 20:20.2% Shen piperazine mycin flutolanil suspending agent (100:1)
Component: Shen piperazine mycin 20%, flutolanil 0.2%, polycarboxylic acids sodium 3%, fatty alcohol oxygen vinethene 3%, ethylene glycol 5%, XG0.35%, silicone defoaming agent 0.3%, Sodium Benzoate 0.6%, soft water complements to 100%.
Preparation method: each component is mixed, mixes through high speed shear, then by sand mill sand milling 2-3 hour, make average grain diameter reach 1-5 micron, 20.2% Shen piperazine mycin flutolanil suspending agent can be obtained.
Comparative example 20:20.2% kasugarnycin flutolanil suspending agent (100:1)
Component: kasugarnycin 20%, flutolanil 0.2%, polycarboxylic acids sodium 3%, fatty alcohol oxygen vinethene 3%, ethylene glycol 5%, XG0.35%, silicone defoaming agent 0.3%, Sodium Benzoate 0.6%, soft water complements to 100%.
Preparation method: the same.
Find through experiment, the Boscalid in above embodiment, mebenil, flutolanil can be replaced arbitrarily, to form new embodiment.No longer carry out this repeating to enumerate, it should be pointed out that for those skilled in the art, under the prerequisite not departing from principle of the present invention, can also make some improvements and modifications, these improvements and modifications also should be considered as protection scope of the present invention.
The pesticidal preparations control graw mold of tomato obtained by above-described embodiment, comparative example, sclerotinia rot of colza test.
1, test process: test is carried out with reference to People's Republic of China's standard (pesticide field efficacy medicine test criterion).This test medicine consumption establishes three concentration for the treatment of respectively according to the difference of each composition, and contrast medicament is commercially available agricultural chemicals 50% Boscalid water dispersible granules respectively, 1% Shen piperazine mycin SC and blank clean water experiment.
2, test method: each plot area is 50m 2, repeat 4 times, the inspection drug effect method before dispenser after Investigation and Prevention is: with blank incidence classification investigation, every community diagonal samples, and often clicks 10 strains, records total strain number, diseased plant number and sick progression.
Adopt following stage division:
0 grade: anosis;
1 grade: lesion area accounts for less than 5% of whole leaf area;
3 grades: lesion area accounts for the 6%-10% of whole leaf area;
5 grades: lesion area accounts for the 11%-20% of whole leaf area;
7 grades: lesion area accounts for the 21%-40% of whole leaf area;
9 grades: lesion area accounts for more than 41% of whole leaf area;
Result of the test sees the following form 4,5:
Table 4 Shen piperazine mycin (A) and the composite control efficiency to graw mold of tomato of Boscalid (B1)
Table 4 field efficacy result shows, within the scope of weight ratio 1:100-100:1, Shen piperazine mycin and Boscalid Complex synergism obvious, particularly within the scope of 1:50-50:1, especially within the scope of 1:25-25:1, have excellent control efficiency to graw mold of tomato, control efficiency is better than single dose kind.
Table 5 embodiment compares with the control efficiency of comparative example to sclerotinia rot of colza
Inventor find other antibiotics bactericide and Boscalid composite, within the scope of weight ratio 1:100-100:1, also there is certain Complex synergism.Because length limit, do not enumerate, only with the representational jinggangmeisu of most, kasugarnycin, polyoxin constructs comparative example and embodiment carries out preventive effect contrast, as table 5 field efficacy result shows, within the scope of weight ratio 1:100-100:1, Shen piperazine mycin and Boscalid Complex synergism obvious, particularly within the scope of 1:50-50:1, within the scope of 1:25-25:1, more obvious to the control efficiency synergy of plant disease, Shen piperazine mycin and Boscalid Complex synergism are better than other antibiotics bactericide and the composite control efficiency of Boscalid.
Other succinic acid suppress series bactericidal agent and Shen piperazine mycin composite, within the scope of weight ratio 1:100-100:1, also there is certain Complex synergism.Because length limit, do not enumerate, only construct comparative example with the representational thifluzamide of most and embodiment carries out preventive effect contrast, as table 5 field efficacy result shows, within the scope of weight ratio 1:100-100:1, Shen piperazine mycin and Boscalid Complex synergism obvious, particularly within the scope of 1:50-50:1, within the scope of 1:25-25:1, more obvious to the control synergy of plant disease, Shen piperazine mycin and Boscalid Complex synergism are better than other succinic acid and suppress series bactericidal agent and the composite control efficiency of Shen piperazine mycin.
Rust of pear, root rotof flax test is prevented and treated with the pesticidal preparations that above-described embodiment, comparative example obtain.
1, test process: test is carried out with reference to People's Republic of China's standard (pesticide field efficacy medicine test criterion).This test medicine consumption establishes three concentration for the treatment of respectively according to the difference of each composition, and contrast medicament is commercially available agricultural chemicals 75%% mebenil wp, 1% Shen piperazine mycin SC and blank clean water experiment respectively.
2, test method: each plot area is 50m 2, repeat 4 times, the inspection drug effect method before dispenser after Investigation and Prevention is: with blank incidence classification investigation, every community diagonal samples, and often clicks 10 strains, records total strain number, diseased plant number and sick progression.
Adopt following stage division:
0 grade: anosis;
1 grade: lesion area accounts for less than 5% of whole leaf area;
3 grades: lesion area accounts for the 6%-10% of whole leaf area;
5 grades: lesion area accounts for the 11%-20% of whole leaf area;
7 grades: lesion area accounts for the 21%-40% of whole leaf area;
9 grades: lesion area accounts for more than 41% of whole leaf area;
Result of the test sees the following form 6,7:
Table 6 Shen piperazine mycin (A) and the composite control efficiency to rust of pear of mebenil (B2)
Table 6 field efficacy result shows, within the scope of weight ratio 1:100-100:1, Shen piperazine mycin and mebenil Complex synergism obvious, particularly within the scope of 1:50-50:1, especially within the scope of 1:25-25:1, have excellent control efficiency to rust of pear, control efficiency is better than single dose kind.
Table 7 embodiment compares with the control efficiency of comparative example to root rotof flax
Inventor find other antibiotics bactericide and mebenil composite, within the scope of weight ratio 1:100-100:1, also there is certain Complex synergism.Because length limit, do not enumerate, only construct comparative example with the representational tetramycin of most and embodiment carries out preventive effect contrast, as table 7 field efficacy result shows, within the scope of weight ratio 1:100-100:1, Shen piperazine mycin and mebenil Complex synergism obvious, particularly within the scope of 1:50-50:1, within the scope of 1:25-25:1, more obvious to the control efficiency synergy of plant disease, Shen piperazine mycin and mebenil Complex synergism are better than other antibiotics bactericide and the composite control efficiency of mebenil.
Other succinic acid suppress series bactericidal agent and Shen piperazine mycin composite, within the scope of weight ratio 1:100-100:1, also there is certain Complex synergism.Because length limit, do not enumerate, only construct comparative example with the representational thifluzamide of most and embodiment carries out preventive effect contrast, as table 7 field efficacy result shows, within the scope of weight ratio 1:100-100:1, Shen piperazine mycin and mebenil Complex synergism obvious, particularly within the scope of 1:50-50:1, within the scope of 1:25-25:1, more obvious to the control efficiency synergy of plant disease, Shen piperazine mycin and mebenil Complex synergism are better than other succinic acid and suppress series bactericidal agent and the composite control efficiency of Shen piperazine mycin.
The pesticidal preparations water prevention rice sheath blight obtained by above-described embodiment, comparative example, rice seedling blight test.
1, test process: test is carried out with reference to People's Republic of China's standard (pesticide field efficacy medicine test criterion).This test medicine consumption establishes three concentration for the treatment of respectively according to the difference of each composition, and contrast medicament is commercially available agricultural chemicals 20% flutolanil WP, 1% Shen piperazine mycin suspending agent and blank clean water experiment respectively.
2, test method: each plot area is 50m 2, repeat 4 times, the inspection drug effect method before dispenser after Investigation and Prevention is: with blank incidence classification investigation, every community diagonal samples, and often clicks 10 strains, records total strain number, diseased plant number and sick progression.
Adopt following stage division:
0 grade: anosis;
1 grade: lesion area accounts for less than 5% of whole leaf area;
3 grades: lesion area accounts for the 6%-10% of whole leaf area;
5 grades: lesion area accounts for the 11%-20% of whole leaf area;
7 grades: lesion area accounts for the 21%-40% of whole leaf area;
9 grades: lesion area accounts for more than 41% of whole leaf area;
Result of the test sees the following form 8,9:
Table 8 Shen piperazine mycin (A) and the composite control efficiency to rice sheath blight disease of flutolanil (B3)
Table 8 field efficacy result shows, within the scope of weight ratio 1:100-100:1, Shen piperazine mycin and flutolanil Complex synergism obvious, particularly within the scope of 1:50-50:1, especially within the scope of 1:25-25:1, have excellent control efficiency to rice sheath blight disease, control efficiency is better than single dose kind.
Table 9 embodiment compares with the control efficiency of comparative example to rice seedling blight
Inventor find other antibiotics bactericide and flutolanil composite, within the scope of weight ratio 1:100-100:1, also there is certain Complex synergism.Because length limit, do not enumerate, only with the representational tetramycin of most, polyoxin, jinggangmeisu, kasugarnycin constructs comparative example and embodiment carries out preventive effect contrast, as table 9 field efficacy result shows, within the scope of weight ratio 1:100-100:1, Shen piperazine mycin and flutolanil Complex synergism obvious, particularly within the scope of 1:50-50:1, within the scope of 1:25-25:1, more obvious to the control efficiency synergistic effect of plant disease, Shen piperazine mycin and flutolanil is composite is better than other antibiotics bactericide and the composite control efficiency of flutolanil.
Other succinic acid suppress series bactericidal agent and Shen piperazine mycin composite, within the scope of weight ratio 1:100-100:1, also there is certain Complex synergism.Because length limit, do not enumerate, only construct comparative example with the representational thifluzamide of most and embodiment carries out preventive effect contrast, as table 9 field efficacy result shows, within the scope of weight ratio 1:100-100:1, Shen piperazine mycin and flutolanil Complex synergism obvious, particularly within the scope of 1:50-50:1, within the scope of 1:25-25:1, more obvious to the control efficiency synergistic effect of plant disease, Shen piperazine mycin and composite other succinic acid that is better than of flutolanil suppress series bactericidal agent and the composite control efficiency of Shen piperazine mycin.
Inventor finds to use succinate dehydrogenase inhibitors series bactericidal agent as Boscalid, mebenil, any one and Shen piperazine mycin in flutolanil are composite, within the scope of weight ratio 1:100-100:1, particularly within the scope of 1:50-50:1, especially within the scope of 1:25-25:1, to downy mildew, gray mold, epidemic disease, late blight, powdery mildew, scab, spot defoliation, net blotch, glume blight, eyeprint is sick, ring spot, brown spot, fusarium wilt, rice blast, anthracnose, canker, banded sclerotial blight, the plant diseases such as stalk break also have obvious synergistic effect.Because length is limited, be not repeated.
The above is the preferred embodiment of the present invention; it should be pointed out that for those skilled in the art, under the prerequisite not departing from principle of the present invention; can also make some improvements and modifications, these improvements and modifications also should be considered as protection scope of the present invention.

Claims (10)

1. a bactericidal composition, is characterized in that, active ingredient contains Shen piperazine mycin and succinate dehydrogenase inhibitors series bactericidal agent, and the weight ratio of described Shen piperazine mycin and succinate dehydrogenase inhibitors series bactericidal agent is 1:100 ~ 100:1.
2. bactericidal composition according to claim 1, is characterized in that, the weight ratio of described Shen piperazine mycin and succinate dehydrogenase inhibitors series bactericidal agent is 1:50 ~ 50:1.
3. bactericidal composition according to claim 2, is characterized in that, the weight ratio of described Shen piperazine mycin and succinate dehydrogenase inhibitors series bactericidal agent is 1:25 ~ 25:1.
4. according to described bactericidal composition arbitrary in claims 1 to 3, it is characterized in that, described succinate dehydrogenase inhibitors series bactericidal agent is one or more of Boscalid, mebenil or flutolanil.
5. according to described bactericidal composition arbitrary in Claims 1-4, it is characterized in that, also containing pesticidal preparations auxiliary element in described bactericidal composition, to make the formulation being applicable to agriculturally using.
6. bactericidal composition according to claim 5, is characterized in that, described pesticidal preparations auxiliary element is one or more in solvent, emulsifier, dispersant, stabilizing agent, antifreezing agent or wetting agent.
7. bactericidal composition according to claim 5, is characterized in that, the formulation of described bactericidal composition is suspending agent, dry suspending agent, water dispersible granules, wetting powder, oil-suspending agent, solvable pulvis or granule.
8. the application of the arbitrary described bactericidal composition of claim 1 to 7 in the pesticidal preparations of preparation controlling plant diseases.
9. the application of bactericidal composition according to claim 8, it is characterized in that, be applied to the pesticidal preparations that preparation prevents and treats downy mildew, gray mold, epidemic disease, late blight, powdery mildew, scab, spot defoliation, net blotch, glume blight, eyeprint disease, ring spot, brown spot, fusarium wilt, rice blast, anthracnose, canker, banded sclerotial blight, stalk break.
10. the bactericidal composition described in claim 1 to 7 is applied to the method for controlling disease, it is characterized in that, active ingredient Shen piperazine mycin and succinate dehydrogenase inhibitors series bactericidal agent are used with above-mentioned weight ratio, and it is used after mixing in proportion, use in proportion simultaneously or use successively in proportion.
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