CN105432514A - Method for improving sea cucumber growth water area environment by adopting compound microbial ecological agent - Google Patents
Method for improving sea cucumber growth water area environment by adopting compound microbial ecological agent Download PDFInfo
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- CN105432514A CN105432514A CN201510757952.2A CN201510757952A CN105432514A CN 105432514 A CN105432514 A CN 105432514A CN 201510757952 A CN201510757952 A CN 201510757952A CN 105432514 A CN105432514 A CN 105432514A
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- sea cucumber
- bacteria
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Abstract
The invention relates to the technical field of aquaculture, in particular to a method for improving a sea cucumber growth water area environment by adopting a compound microbial ecological agent. The method comprises the following steps of: firstly, preparing culture liquids for bacilli, photosynthetic bacteria, nitrobacteria and lactic acid bacteria respectively; secondly, mixing the culture liquids; and finally, splashing a mixed liquid in a sea cucumber culture pond according to a certain usage dosage. The method has the beneficial effects that 1) the use of the method does not cause influence to the dissolved oxygen content and the pH value of the pond, and can remarkably reduce the content of ammonia nitrogen and nitrite in the culture pond; 2) when a mixing ratio of the bacilli to the photosynthetic bacteria to the nitrobacteria to the lactic acid bacteria is 1 : 2 : 10 : 2, the growth speed of sea cucumbers is increased by more than 20%; and 3) the method is suitable for commercial popularization and application, and has wide commercial prospects.
Description
Technical field
The present invention relates to technical field of aquaculture, particularly relate to the method for ammoniacal nitrogen in a kind of effective reduction sea cucumber growth waters.
Background technology
In holothruian cultures process, increasing of the materials such as the ammonia nitrogen in waters, the problems such as cultivate water is seriously polluted, disease frequently occurs, culturing sea cucumbers lethality increases can be caused, if rely on chemosterilant and antibiotic medicine, then can cause chemical residual excessive problem in sea cucumber body, hinder the sound development of holothruian cultures industry.In order to address these problems, add this microbial bacteria of nitrifying bacteria to holothruian cultures waters and improve the development trend that water quality becomes current, but the various method openly reported at present and practical application show that in every premium on currency, ammonia nitrogen content can only reduce 20-30%, this can not meet actual needs.
Summary of the invention
In order to overcome above-mentioned the deficiencies in the prior art, the object of this invention is to provide the method for ammoniacal nitrogen in a kind of effective reduction sea cucumber growth waters.
Technical scheme of the present invention is: a kind of method of ammoniacal nitrogen in effective reduction sea cucumber growth waters, comprises the steps: 1) open aerator, make the dissolved oxygen amount in water body reach 5-7mg/L; 2) under rather dark condition, in sea cucumber culture pond, evenly threw in the nitrifying bacteria that concentration is 90-200 hundred million cells/L every 5 days, throw in three times altogether; 3) third time throws in and terminates after 7-10 days, under rather dark condition, evenly throws in the nitrifying bacteria that concentration is 8,000,000,000 cells/L for the 4th time in sea cucumber culture pond.
Step 2) in injected volume be that every mu of water surface throws in 1.5kg nitrifying bacteria.
Step 3) in injected volume be that every mu of water surface throws in 2kg nitrifying bacteria.
Step 2) in release time be at night after 7.
The invention has the beneficial effects as follows: 1) in every premium on currency, ammonia nitrogen content reduces by more than 50%, and water quality environment obviously improves, and is more suitable for sea cucumber growth; 2) be applicable to apply, there is very large commercial value.
Embodiment
Embodiment one
In effective reduction sea cucumber growth waters, a method for ammoniacal nitrogen, comprises the steps:
1) open aerator, make the dissolved oxygen amount in water body reach 5mg/L.
2), at night after 7, in sea cucumber culture pond, evenly threw in the nitrifying bacteria that concentration is 9,000,000,000 cells/L every 5 days, throw in three times altogether, every mu of water surface throws in 1.5kg nitrifying bacteria at every turn.First time detects water quality after throwing in and terminating, and in every premium on currency, ammonia nitrogen content reduced 20% before throwing in; After second time is thrown in and terminated, in every premium on currency, ammonia nitrogen content reduced 30% before throwing in; Third time reduces 40% before the input of ammonia nitrogen content ratio in every premium on currency after throwing in and terminating
3) third time throws in end after 7 days, and at night after 7, in sea cucumber culture pond, evenly throw in the nitrifying bacteria that concentration is 8,000,000,000 cells/L the 4th time, every mu of water surface throws in 2kg nitrifying bacteria.Throw in for 4th time after terminating, in every premium on currency, ammonia nitrogen content reduced 55% before throwing in.
Embodiment two
In effective reduction sea cucumber growth waters, a method for ammoniacal nitrogen, comprises the steps:
1) open aerator, make the dissolved oxygen amount in water body reach 5mg/L.
2), at night after 7, in sea cucumber culture pond, evenly threw in the nitrifying bacteria that concentration is 10,000,000,000 cells/L every 5 days, throw in three times altogether, every mu of water surface throws in 1.5kg nitrifying bacteria at every turn.First time detects water quality after throwing in and terminating, and in every premium on currency, ammonia nitrogen content reduced 20% before throwing in; After second time is thrown in and terminated, in every premium on currency, ammonia nitrogen content reduced 40% before throwing in; Third time reduces 50% before the input of ammonia nitrogen content ratio in every premium on currency after throwing in and terminating
3) third time throws in end after 10 days, and at night after 7, in sea cucumber culture pond, evenly throw in the nitrifying bacteria that concentration is 8,000,000,000 cells/L the 4th time, every mu of water surface throws in 2kg nitrifying bacteria.Throw in for 4th time after terminating, in every premium on currency, ammonia nitrogen content reduced 90% before throwing in.
The content utilizing the method disclosed in the present that sea cucumber can be made to grow ammoniacal nitrogen in waters reduces by more than 50%, greatly improves water quality environment, is more conducive to the cultivation of sea cucumber, have wide commercial promise.
Above one embodiment of the present of invention have been described in detail, but described content being only preferred embodiment of the present invention, can not being considered to for limiting practical range of the present invention.All equalizations done according to the present patent application scope change and improve, and all should still belong within patent covering scope of the present invention.
Claims (5)
1. adopt compound micro-ecological preparation to improve a method for sea cucumber growth water environment, it is characterized in that comprising the steps:
1) bacillus expansion cultivation-ripe bacterium mud is loaded triangular flask, vibrating dispersion bacterium mud, obtain uniform bacteria suspension, then bacteria suspension is heated 5min in 85 DEG C of water-baths, with the inoculum concentration of volume ratio 5% access 100L seeding tank, loading amount coefficient is 60%, speed of agitator 150r/min, throughput l-2m
3/ h, cultivates 17h for 37 DEG C, then seed culture fluid is accessed lm with the inoculum concentration of volume ratio 10%
3fermentation tank, loading amount coefficient is 65%, speed of agitator 150r/min, throughput 1.5m
3/ h, cultivates 20h for 37 DEG C;
2) photosynthetic bacteria expands cultivations-test tube or small-sized saline bottle culture fluid and accesses 5L triangular flask with 10% inoculum concentration, and loading amount coefficient is 80%, 2000Lx illumination, 28-30 DEG C of quiescent culture 5 days; Then seed culture fluid is accessed 20 50 kilograms of Plastic Drums with the inoculum concentration of volume ratio 10%, loading amount coefficient is 80%, indoor controlled light 2000Lx, temperature 28-30 DEG C, and quiescent culture 5-10 days is kermesinus to culture fluid, has a large amount of bacterial sediment at the bottom of bucket;
3) nitrifying bacteria expands cultivation-triangular flask bacterium liquid with the inoculum concentration of volume ratio 5% access l00L seeding tank, and loading amount coefficient is 60%, speed of agitator 150r/min, throughput 0.5m
3cultivate 5d for/h, 30-37 DEG C; Then seed culture fluid is met lm with the inoculum concentration of volume ratio 5%
3fermentation tank, loading amount coefficient is 60%, speed of agitator 150r/min, throughput 0.5-2m
3/ h, cultivates 5d for 28 DEG C;
4) lactic acid bacteria expands cultivation-triangular flask bacterium liquid with the inoculum concentration of volume ratio 5% access 100L seeding tank, and loading amount coefficient is 70%, speed of agitator 100r/min, throughput 0.5m
3cultivate 48h for/h, 30-40 DEG C; Then seed culture fluid is met lm with the inoculum concentration of volume ratio 5%
3fermentation tank, loading amount coefficient is 70%, speed of agitator 150r/min, throughput lm
3/ h, cultivates 35-48h for 40 DEG C;
5) mix-by step 1) to step 4) in obtained successively bud robe bacillus, photosynthetic bacteria, nitrifying bacteria, lactic acid bacteria mixing, mixed proportion is 1:(1-3): (5-10): 2.
6) mixed liquor is splashed into sea cucumber culture pond.
2. according to claim 1 a kind of adopt compound micro-ecological preparation improve sea cucumber growth water environment method, it is characterized in that: described step 5) in mixed proportion be: bud pole bacterium, photosynthetic bacteria, nitrifying bacteria, lactic acid bacteria are 1:1:5:2.
3. according to claim 1 a kind of adopt compound micro-ecological preparation improve sea cucumber growth water environment method, it is characterized in that: described step 5) in mixed proportion be: bud pole bacterium, photosynthetic bacteria, nitrifying bacteria, lactic acid bacteria are 1:2:5:2.
4. according to claim 1 a kind of adopt compound micro-ecological preparation improve sea cucumber growth water environment method, it is characterized in that: described step 5) in mixed proportion be: bud pole bacterium, photosynthetic bacteria, nitrifying bacteria, lactic acid bacteria are 1:2:10:2.
5. according to claim 1 a kind of adopt compound micro-ecological preparation improve sea cucumber growth water environment method, it is characterized in that: described step 6) in mixed liquor splash into sea cucumber culture pond with the using dosage of 0.3-0.6mg/L.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510757952.2A CN105432514A (en) | 2015-11-09 | 2015-11-09 | Method for improving sea cucumber growth water area environment by adopting compound microbial ecological agent |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510757952.2A CN105432514A (en) | 2015-11-09 | 2015-11-09 | Method for improving sea cucumber growth water area environment by adopting compound microbial ecological agent |
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| CN105432514A true CN105432514A (en) | 2016-03-30 |
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| CN201510757952.2A Pending CN105432514A (en) | 2015-11-09 | 2015-11-09 | Method for improving sea cucumber growth water area environment by adopting compound microbial ecological agent |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109336263A (en) * | 2018-10-30 | 2019-02-15 | 江苏世邦生物工程科技有限公司 | Microorganism water conditioner and preparation method thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1844364A (en) * | 2006-05-11 | 2006-10-11 | 江苏省微生物研究所有限责任公司 | Microecological preparation-tetragenous viable bacteria preparation and method for preparing same |
| CN104556403A (en) * | 2014-12-29 | 2015-04-29 | 天津振邦水产养殖有限公司 | Method for reducing eutrophication in sea cucumber growth waters |
-
2015
- 2015-11-09 CN CN201510757952.2A patent/CN105432514A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1844364A (en) * | 2006-05-11 | 2006-10-11 | 江苏省微生物研究所有限责任公司 | Microecological preparation-tetragenous viable bacteria preparation and method for preparing same |
| CN104556403A (en) * | 2014-12-29 | 2015-04-29 | 天津振邦水产养殖有限公司 | Method for reducing eutrophication in sea cucumber growth waters |
Non-Patent Citations (1)
| Title |
|---|
| 何义进: "微生态制剂降解养殖水体氨氮及亚硝酸盐的研究", 《中国优秀硕士学位论文全文数据库 工程科技Ⅰ辑》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109336263A (en) * | 2018-10-30 | 2019-02-15 | 江苏世邦生物工程科技有限公司 | Microorganism water conditioner and preparation method thereof |
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Application publication date: 20160330 |
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