CN108264152A - A kind of preparation method containing a variety of microbial inoculum biologic packing materials - Google Patents
A kind of preparation method containing a variety of microbial inoculum biologic packing materials Download PDFInfo
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- CN108264152A CN108264152A CN201810301484.1A CN201810301484A CN108264152A CN 108264152 A CN108264152 A CN 108264152A CN 201810301484 A CN201810301484 A CN 201810301484A CN 108264152 A CN108264152 A CN 108264152A
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/347—Use of yeasts or fungi
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/348—Biological treatment of water, waste water, or sewage characterised by the microorganisms used characterised by the way or the form in which the microorganisms are added or dosed
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2305/00—Use of specific compounds during water treatment
- C02F2305/06—Nutrients for stimulating the growth of microorganisms
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Abstract
The invention discloses a kind of preparation methods containing a variety of microbial inoculum biologic packing materials, belong to biologic packing material preparing technical field.The nutriment of mixed bacteria abundance is supplied in the present invention using corn stalk powder as natural nutrition material,Shale has layer structure in the modified Nano mixture of preparation,Flyash is water absorbing porous material,The biologic packing material of the present invention contains three classes microbial bacterial agent,One is the industrial wastewater microbial inoculum prepared during filler matrix contained by pool bottom sludge,Secondly the microbial inoculum obtained by filler matrix dipping mixed bacteria liquid,Thirdly impregnate the microbial film obtained in animal farm wastewater for filler matrix,Symbiosis is coordinated between three kinds of different type strains,Mutual not antagonism,It is active high,Biomass is big,Breeding is fast,In addition the microorganism after the biomass membrane breakage on biologic packing material surface inside biologic packing material can be such that bacteria suspension concentration in sewage around increases within a certain period of time instead by breeding dispersal,So as to which dirty Organic substance in water be made efficiently to be handled,It has a extensive future.
Description
Technical field
The invention discloses a kind of preparation methods containing a variety of microbial inoculum biologic packing materials, belong to biologic packing material technology of preparing neck
Domain.
Background technology
Biologic packing material is divided into composite filling, 3 D elastic filler, porous suspended ball filler, Active biological filler etc..It is aerobic
Stereo hollow filler is used in organism fluidization bed process as aerobic bio-carrier, filler is hollow structure, and filler suspends in water.
Filler growth inside anaerobic bacteria, generating denitrification can be with denitrogenation；External growth aerobic bacteria, organics removal are entire to handle
Nitrification and denitrification process is existed simultaneously in the process.
Rapid growth and industrialized propulsion with the size of population, the pollution level getting worse of water body, and occur
Organic difficult decomposed substance and traditional ammonia nitrogen, the novel pollution type of COD compound pollutions, traditional wastewater processing method is to such
The treatment effect of sewage is generally bad.The research that processing is current water treatment field is carried out to polluted-water using microorganism
Hot spot direction and a kind of green high-efficient, the processing means of non-secondary pollution.Wherein biofilm is in filler surface biofilm
It is degraded by the micropopulation on biomembrane to the pollutant in sewage.Stuffing process is to utilize the adsorbable biota in its surface
Body makes microorganism fixed load on the surface thereof, so as to reach wastewater treatment efficiency.But the most of surfaces of filler used at present
Can be relatively low, in use, be not easy to sprawl when liquid flows through its surface to form film, but formed dispersion liquid pearl or
Tiny channel so that filler individual lower part is difficult to effectively be soaked, so as to largely affect mass-transfer efficiency.
Biologic packing material used at present is all different degrees of, and there is defects.Present immobilized microorganism material is universal
There are stability is poor, microorganism colonization poor performance, mechanical property is poor the characteristics of.Due to the fluctuation of drainage flow and water quality,
The microorganism added is easy to be lost in or be preyed on by protozoan, it is difficult to keep steady and sustained removal effect.It is fixed to fill out
Expect that specific surface area is small, biofilm biomass is few, and surface is smooth, biomembrane is easy to fall off.Removal of the microbial method to difficult decomposing organic matter
It is ineffective.
Therefore, invention is a kind of has positive effect containing a variety of microbial inoculum biologic packing materials to biologic packing material preparing technical field.
Invention content
Present invention mainly solves the technical issues of, for due to the fluctuation of drainage flow and water quality, what is added is micro- at present
Biology is easy to be lost in or be preyed on by protozoan, it is difficult to keep steady and sustained removal effect, fixed filler specific surface
Product is small, biofilm biomass is few, and surface is smooth, and biomembrane is easy to fall off, and microbial method is bad to the removal effect of difficult decomposing organic matter
The defects of, provide a kind of preparation method containing a variety of microbial inoculum biologic packing materials.
In order to solve the above-mentioned technical problem, the technical solution adopted in the present invention is：
A kind of preparation method containing a variety of microbial inoculum biologic packing materials, it is characterised in that specifically preparation process is：
（1）It counts in parts by weight, 20~30 parts of maize straws is placed in pulverizer and are crushed, sieve with 100 mesh sieve to obtain maize straw
Powder weighs 20~30 parts of agar, 2~4 parts of dusty yeasts, 5~7 parts of wheat bran, 20~25 parts of corn stalk powders and is placed in beaker, water-bath
Heating and thermal insulation obtains microbiological culture media；
（2）Nitrococcus, saccharomycete and the strain of bacillus subtilis are mixed, mixed bacteria is obtained, to microbiological culture media
Middle access mixed bacteria, culture, obtains mixed bacteria culture；
（3）It counts in parts by weight, by 10~12 parts of peptones, 8~10 parts of glucose, 10~15 parts of yeast extracts, 50~55 parts of lifes
Reason brine is placed in culture dish, is stirred 3~5min, obtains diffusion culture medium, then by mixed bacteria culture, be inoculated into expansion
It dissipates in culture medium, after inoculation, diffusion culture medium is placed in the conical flask with ventilation device, opens magnetic stirrer,
It is cultivated under the conditions of gravity-flow ventilation, obtains mixed bacterial liquid；
（4）Count in parts by weight, by 40~50 parts of flyash, 10~20 parts of quick limes, 5~10 parts of nano aluminium oxides, 1~15 part
Sodium metasilicate and 4~5 parts of shale mixings, are granulated, obtain raw material ball, raw material ball are placed in baking oven after drying, and heating is forged
It burns, is subsequently moved within resistance kiln roasting, obtains modified Nano mixture；
（5）Aeration tank is filled into aluminium metallurgy factory industrial wastewater, carries out bored exposure, stops aeration, staticly settles, discharges upper strata waste water, and
Same amount of fresh sewage is injected, addition modified Nano mixture is straight into aeration tank, and standing sedimentation salvages pool bottom sludge, will
Compression moulding in plastic mould, which is put into, after pool bottom sludge drying % obtains filler matrix；
（6）Filler matrix is placed in mixed bacterial liquid after impregnating and taken out, be subsequently placed into animal farm wastewater and impregnate biofilm, until
Filler matrix surface has one layer of biomass membrane, obtains containing a variety of microbial inoculum biologic packing materials.
Step（1）The specification of being sieved is 100 mesh, and temperature is 90~100 DEG C after heating water bath, soaking time 10
~15min.
Step（2）Nitrococcus, saccharomycete and the strain of the bacillus subtilis mixing quality ratio is 1 ︰, 1 ︰ 3, is mixed
5~8% that strain cut-in quality is microbiological culture media quality are closed, cultivation temperature is 30~35 DEG C, and incubation time is 18~20h.
Step（3）The mass fraction of the physiological saline is 0.9%, and mixed bacteria culture inoculation quality is trained for diffusion
The 8~10% of matrix amount are supported, speed of agitator is 300~350r/min, and cultivation temperature is 30~33 DEG C, is trained under the conditions of gravity-flow ventilation
It is 16~18h to support the time.
Step（4）Described is placed in raw material ball in the baking oven that temperature is 70~80 DEG C after drying, temperature after heating 400
DEG C, calcination time is 30~40min, and calcination temperature is 1000~1100 DEG C, and roasting time is 2~3h.
Step（5）The bored exposure time is 4~5 days, staticly settles the time as 1~2h, and discharge upper strata waste water is in pond
The 1/4~1/5 of water volume, into aeration tank add in modified Nano mixture until in aeration tank solids content concn reach 5~
10g/L, standing sedimentation time are 2~3 weeks, and water content is 5~10% after pool bottom sludge drying, and pressing pressure is 7~8MPa.
Step（6）Filler matrix dip time in mixed bacterial liquid is 4~5 days, and animal farm wastewater solid contains
It is 4~5% to measure, and the dipping biofilm time is 20~25 days.
The beneficial effects of the invention are as follows：
（1）For the present invention using maize straw as raw material, crushing obtains corn stalk powder, adds in other nutriments and micro- life is prepared
Object culture medium is mixed being cultivated in nitrococcus, saccharomycete and the strain of bacillus subtilis access microbiological culture media
Strain prepares the diffusion culture medium of high nutrition for cultivating mixed bacteria, obtains mixed bacterial liquid, with flyash, quick lime, receive
The raw materials mixing such as rice aluminium oxide, sodium metasilicate and shale is granulated, and obtains raw material ball, by calcining and roasting obtains modified Nano mixing
Material, modified Nano mixture is poured into the aluminum smelting industry waste water through bored exposure, and standing sedimentation salvages pool bottom sludge after a certain period of time,
To impregnate mixed bacterial liquid after sludge compression moulding successively and breeding wastewater obtain high absorption composite biological packing, in the present invention with
Corn stalk powder is supplied to the nutriment of mixed bacteria abundance for natural nutrition material, especially conducive to the quick numerous of bacillus subtilis
It grows, so as to obtain the microorganism species of high concentration, there is shale layer structure, flyash to be in the modified Nano mixture of preparation
Water absorbing porous material contributes to filler matrix to be capable of the wetting of large area and adsorb the microorganism of high concentration, and nano aluminium oxide can
Promote the refinement of powder in filler, and play complexing bond effect, make filler matrix resistance to erosion, remain fixed form, so as to make
Microorganism adsorption content is high, not easily runs off；
（2）The biologic packing material of the present invention contains three classes microbial bacterial agent, and one is pool bottom sludge institute during preparation filler matrix
The industrial wastewater microbial inoculum contained, secondly the microbial inoculum obtained by filler matrix dipping mixed bacteria liquid, thirdly being supported for filler matrix dipping
Grow the microbial film obtained in waste water, between three kinds of different type strains symbiosis coordinate, mutual not antagonism, activity is high, biology
Amount is big, and breeding is fast, and biologic packing material is added in sewage disposal system, and microbial bacterial agent and filler synergy, filler is to bacterium
Agent has immobilization role that can simultaneously increase microbial inoculum active area and range, and microbial inoculum has efficient degradation ability to pollutant in water body,
And filler surface has a large amount of Si-O-Si keys and Al-O-Al keys that can generate dipole, dipole with the deleterious molecular with certain polarity
In the absorption of key, anion and flyash ion is formed between the silicates such as positively charged alumina silicate, calcium silicates and ferrosilite
It exchanges or ionic adsorption, some ingredients in flyash can also be acted on the pollutant in sewage make its flocculation sediment, with fine coal
Ash forms the synergistic effect of absorption and flocculation sediment, in addition after the biomass membrane breakage on biologic packing material surface inside biologic packing material
Microorganism can be such that bacteria suspension concentration in sewage around increases within a certain period of time instead by breeding dispersal, organic in sewage so as to make
Object is efficiently handled, and is had a extensive future.
Specific embodiment
It counts in parts by weight, 20~30 parts of maize straws is placed in pulverizer and are crushed, sieve with 100 mesh sieve to obtain maize straw
Powder weighs 20~30 parts of agar, 2~4 parts of dusty yeasts, 5~7 parts of wheat bran, 20~25 parts of corn stalk powders and is placed in beaker, water-bath
90~100 DEG C are heated to, keeps the temperature 10~15min microbiological culture medias；By nitrococcus, saccharomycete and the bacterium of bacillus subtilis
Kind 1 ︰ in mass ratio, 1 ︰ 3 are mixed, and obtain mixed bacteria, the mixed bacteria of its quality 5~8% is accessed into microbiological culture media,
18~20h is cultivated under the conditions of 30~35 DEG C, obtains mixed bacteria culture；It counts in parts by weight, by 10~12 parts of peptones, 8
~10 parts of glucose, 10~15 parts of yeast extracts, 50~55 parts of mass fractions are placed in for 0.9% physiological saline in culture dish, and stirring is mixed
3~5min is closed, diffusion culture medium, then the mixed bacteria culture by its quality 8~10% is obtained, is inoculated into diffusion culture medium,
After inoculation, diffusion culture medium is placed in the conical flask with ventilation device, magnetic stirring apparatus is opened, with 300~350r/min
Rotating speed stirring, under the conditions of 30~33 DEG C, gravity-flow ventilation cultivate 16~18h, obtain mixed bacterial liquid；It counts in parts by weight,
40~50 parts of flyash, 10~20 parts of quick limes, 5~10 parts of nano aluminium oxides, 1~15 part of sodium metasilicate and 4~5 parts of shale are mixed
It is even, it is granulated, obtains raw material ball, raw material ball is placed in the baking oven that temperature is 70~80 DEG C after drying, be heated to 400 DEG C,
30~40min is calcined, is subsequently moved in resistance furnace with 1000~1100 DEG C of 2~3h of temperature calcination, obtains modified Nano mixing
Material；Aeration tank is filled into aluminium metallurgy factory industrial wastewater, carries out bored exposure, stops aeration after 4~5 days, staticly settles 1~2h, discharges pond
Interior 1/5~1/4 upper strata waste water, and inject same amount of fresh sewage, into aeration tank add in modified Nano mixture until
Solids content concn reaches 5~10 g/l in aeration tank, and standing sedimentation 2~3 weeks salvages pool bottom sludge, pool bottom sludge is dried
It is put into plastic mould after being 5~10% to water content, filler matrix is obtained with 7~8MPa pressure compression mouldings；By filler matrix
It is placed in mixed bacterial liquid after impregnating 4~5 days and takes out, be subsequently placed into impregnate in the animal farm wastewater that solid content is 4~5% and hang
Film 20~25 days, until filler matrix surface is obtained with one layer of biomass membrane containing a variety of microbial inoculum biologic packing materials.
Example 1
It counts in parts by weight, 20 parts of maize straws is placed in pulverizer and are crushed, sieves with 100 mesh sieve to obtain corn stalk powder, weigh
20 parts of agar, 2 parts of dusty yeasts, 5 parts of wheat bran, 20 parts of corn stalk powders are placed in beaker, and heating water bath keeps the temperature 10min to 90 DEG C
Microbiological culture media；1 ︰ 3 of nitrococcus, saccharomycete and the strain of bacillus subtilis 1 ︰ in mass ratio are mixed, are mixed
Strain accesses the mixed bacteria of its quality 5% into microbiological culture media, cultivates 18h under the conditions of 30 DEG C, obtains mixed bacteria
Culture；It counts in parts by weight, by 10 parts of peptones, 8 parts of glucose, 10 parts of yeast extracts, 50 parts of mass fractions are 0.9% physiology
Brine is placed in culture dish, is stirred 3min, is obtained diffusion culture medium, then the mixed bacteria culture by its quality 8%, is connect
In kind to diffusion culture medium, after inoculation, diffusion culture medium is placed in the conical flask with ventilation device, opens magnetic agitation
Device is stirred with the rotating speed of 300r/min, cultivates 16h under the conditions of 30 DEG C, gravity-flow ventilation, obtain mixed bacterial liquid；By weight
Number meter, by 40 parts of flyash, 10 parts of quick limes, 5 parts of nano aluminium oxides, 1 part of sodium metasilicate and 4 parts of shale mixings, is granulated, is obtained
To raw material ball, raw material ball is placed in the baking oven that temperature is 70 DEG C after drying, is heated to 400 DEG C, calcines 30min, be subsequently moved within
With 1000 DEG C of temperature calcination 2h in resistance furnace, modified Nano mixture is obtained；Aeration tank is filled into aluminium metallurgy factory industrial wastewater, into
The bored exposure of row, stops aeration after 4 days, staticly settle 1h, discharges in pond 1/5 upper strata waste water, and injects same amount of fresh dirt
Water, into aeration tank add in modified Nano mixture until aeration tank in solids content concn reach 5 g/l, standing sedimentation 2
Week pool bottom sludge is salvaged, pool bottom sludge is dried to after water content is 5% and is put into plastic mould, with 7MPa pressure compression mouldings
Obtain filler matrix；Filler matrix is placed in mixed bacterial liquid after impregnating 4 days and taken out, be subsequently placed into solid content be 4% support
It grows and biofilm is impregnated in waste water 20 days, until filler matrix surface obtains filling out containing a variety of microbial inoculum biologies with one layer of biomass membrane
Material.
Example 2
It counts in parts by weight, 25 parts of maize straws is placed in pulverizer and are crushed, sieves with 100 mesh sieve to obtain corn stalk powder, weigh
25 parts of agar, 3 parts of dusty yeasts, 6 parts of wheat bran, 22 parts of corn stalk powders are placed in beaker, and heating water bath keeps the temperature 12min to 95 DEG C
Microbiological culture media；1 ︰ 3 of nitrococcus, saccharomycete and the strain of bacillus subtilis 1 ︰ in mass ratio are mixed, are mixed
Strain accesses the mixed bacteria of its quality 7% into microbiological culture media, cultivates 19h under the conditions of 32 DEG C, obtains mixed bacteria
Culture；It counts in parts by weight, by 11 parts of peptones, 9 parts of glucose, 12 parts of yeast extracts, 52 parts of mass fractions are 0.9% physiology
Brine is placed in culture dish, is stirred 4min, is obtained diffusion culture medium, then the mixed bacteria culture by its quality 9%, is connect
In kind to diffusion culture medium, after inoculation, diffusion culture medium is placed in the conical flask with ventilation device, opens magnetic agitation
Device is stirred with the rotating speed of 320r/min, cultivates 17h under the conditions of 32 DEG C, gravity-flow ventilation, obtain mixed bacterial liquid；By weight
Number meter, by 45 parts of flyash, 15 parts of quick limes, 7 parts of nano aluminium oxides, 7 parts of sodium metasilicate and 4 parts of shale mixings, is granulated, is obtained
To raw material ball, raw material ball is placed in the baking oven that temperature is 75 DEG C after drying, is heated to 400 DEG C, calcines 35min, be subsequently moved within
With 1050 DEG C of temperature calcination 2.5h in resistance furnace, modified Nano mixture is obtained；Aeration tank is filled into aluminium metallurgy factory industrial wastewater,
Bored exposure is carried out, stops aeration after 4 days, staticly settles 1.5h, discharges in pond 1/5 upper strata waste water, and is injected same amount of fresh
Sewage, into aeration tank add in modified Nano mixture until aeration tank in solids content concn reach 7 g/l, standing sedimentation 2
Week pool bottom sludge is salvaged, pool bottom sludge is dried to after water content is 7% and is put into plastic mould, with 7MPa pressure compression mouldings
Obtain filler matrix；Filler matrix is placed in mixed bacterial liquid after impregnating 4 days and taken out, be subsequently placed into solid content be 4% support
It grows and biofilm is impregnated in waste water 22 days, until filler matrix surface obtains filling out containing a variety of microbial inoculum biologies with one layer of biomass membrane
Material.
Example 3
It counts in parts by weight, 30 parts of maize straws is placed in pulverizer and are crushed, sieves with 100 mesh sieve to obtain corn stalk powder, weigh
30 parts of agar, 4 parts of dusty yeasts, 7 parts of wheat bran, 25 parts of corn stalk powders are placed in beaker, and heating water bath keeps the temperature 15min to 100 DEG C
Microbiological culture media；1 ︰ 3 of nitrococcus, saccharomycete and the strain of bacillus subtilis 1 ︰ in mass ratio are mixed, are mixed
Strain accesses the mixed bacteria of its quality 8% into microbiological culture media, cultivates 20h under the conditions of 35 DEG C, obtains mixed bacteria
Culture；It counts in parts by weight, by 12 parts of peptones, 10 parts of glucose, 15 parts of yeast extracts, 55 parts of mass fractions are 0.9% life
Reason brine is placed in culture dish, is stirred 5min, obtains diffusion culture medium, then the mixed bacteria culture by its quality 10%,
It is inoculated into diffusion culture medium, after inoculation, diffusion culture medium is placed in the conical flask with ventilation device, opens magnetic agitation
Device is stirred with the rotating speed of 350r/min, cultivates 18h under the conditions of 33 DEG C, gravity-flow ventilation, obtain mixed bacterial liquid；By weight
Number meter, 50 parts of flyash, 20 parts of quick limes, 10 parts of nano aluminium oxides, 15 parts of sodium metasilicate and 5 parts of shale mixings are granulated,
Raw material ball is obtained, raw material ball is placed in the baking oven that temperature is 80 DEG C after drying, is heated to 400 DEG C, calcined 40min, then move
Enter in resistance furnace with 1100 DEG C of temperature calcination 3h, obtain modified Nano mixture；Aeration tank is filled into aluminium metallurgy factory industrial wastewater,
Bored exposure is carried out, stops aeration after 5 days, staticly settles 2h, discharges in pond 1/5 upper strata waste water, and injects same amount of fresh dirt
Water, into aeration tank add in modified Nano mixture until aeration tank in solids content concn reach 10 g/l, standing sedimentation 3
Week pool bottom sludge is salvaged, pool bottom sludge is dried to after water content is 10% and is put into plastic mould, is pressed into 8MPa pressure
Type obtains filler matrix；Filler matrix is placed in mixed bacterial liquid after impregnating 5 days and taken out, it is 5% to be subsequently placed into solid content
Biofilm is impregnated in animal farm wastewater 25 days, until filler matrix surface is obtained with one layer of biomass membrane containing a variety of microbial inoculums biology
Filler.
Comparative example
It is given birth to as a comparison case to produced by the present invention containing a variety of microbial inoculums containing a variety of microbial inoculum biologic packing materials with the production of Yixing company
Performance detection is carried out containing a variety of microbial inoculum biologic packing materials in object filler and comparative example, testing result is as shown in table 1：
Test method：
The test of COD removal rates is detected using ultraviolet-uisible spectrophotometer；
NH3The test of-N removal rates is detected using ultraviolet-uisible spectrophotometer；
The test of TP removal rates is detected using ultraviolet-uisible spectrophotometer；
The test of SS removal rates is dried weight reduction using drying box and is detected；
H2The test of S removal rates is detected using gas-phase molecular absorption spectrometry method；
NH3Removal rate test is detected using nessler reagent spectrophotometer；
Microorganism colonization measures examination and is detected by standard.
1 biologic packing material performance measurement result of table
| Test event | Example 1 | Example 2 | Example 3 | Comparative example |
| COD removal rates（%） | 93.8 | 94.2 | 94.8 | 81.4 |
| NH3 -N removal rates（%） | 67.1 | 67.8 | 68.4 | 35.4 |
| TP removal rates（%） | 97.45 | 98.12 | 98.99 | 84.31 |
| SS removal rates（%） | 96.42 | 97.12 | 97.65 | 81.24 |
| H2S removal rates（%） | 44.75 | 45.12 | 45.88 | 22.65 |
| NH3Removal rate（%） | 56.18 | 57.62 | 58.45 | 36.45 |
| Microorganism colonization amount increase rate（%） | 60 | 62 | 65 | 32 |
The removal produced by the present invention containing the water outlet of a variety of microbial inoculum biologic packing materials COD, SS, TP is relatively aobvious according to above-mentioned middle data
It writes, removal rate reaches more than 90%, reaches《Livestock and poultry breeding industry pollutant emission standard》（GB18596-2001）And farmland irrigating
Irrigate water standard（GB5084-2005）, H2S and NH3Removal rate respectively reach 45.88% and 58.45%, reduce pig farm
Odorant concentration of emission, removal effect is good, and microorganism colonization amount improves 65%, has excellent microorganism colonization performance, tool
Have broad application prospects.
Claims (7)
1. a kind of preparation method containing a variety of microbial inoculum biologic packing materials, it is characterised in that specifically preparation process is：
（1）It counts in parts by weight, 20~30 parts of maize straws is placed in pulverizer and are crushed, sieve with 100 mesh sieve to obtain maize straw
Powder weighs 20~30 parts of agar, 2~4 parts of dusty yeasts, 5~7 parts of wheat bran, 20~25 parts of corn stalk powders and is placed in beaker, water-bath
Heating and thermal insulation obtains microbiological culture media；
（2）Nitrococcus, saccharomycete and the strain of bacillus subtilis are mixed, mixed bacteria is obtained, to microbiological culture media
Middle access mixed bacteria, culture, obtains mixed bacteria culture；
（3）It counts in parts by weight, by 10~12 parts of peptones, 8~10 parts of glucose, 10~15 parts of yeast extracts, 50~55 parts of lifes
Reason brine is placed in culture dish, is stirred 3~5min, obtains diffusion culture medium, then by mixed bacteria culture, be inoculated into expansion
It dissipates in culture medium, after inoculation, diffusion culture medium is placed in the conical flask with ventilation device, opens magnetic stirrer,
It is cultivated under the conditions of gravity-flow ventilation, obtains mixed bacterial liquid；
（4）Count in parts by weight, by 40~50 parts of flyash, 10~20 parts of quick limes, 5~10 parts of nano aluminium oxides, 1~15 part
Sodium metasilicate and 4~5 parts of shale mixings, are granulated, obtain raw material ball, raw material ball are placed in baking oven after drying, and heating is forged
It burns, is subsequently moved within resistance kiln roasting, obtains modified Nano mixture；
（5）Aeration tank is filled into aluminium metallurgy factory industrial wastewater, carries out bored exposure, stops aeration, staticly settles, discharges upper strata waste water, and
Same amount of fresh sewage is injected, addition modified Nano mixture is straight into aeration tank, and standing sedimentation salvages pool bottom sludge, will
Compression moulding in plastic mould, which is put into, after pool bottom sludge drying % obtains filler matrix；
（6）Filler matrix is placed in mixed bacterial liquid after impregnating and taken out, be subsequently placed into animal farm wastewater and impregnate biofilm, until
Filler matrix surface has one layer of biomass membrane, obtains containing a variety of microbial inoculum biologic packing materials.
2. a kind of preparation method containing a variety of microbial inoculum biologic packing materials according to claim 1, it is characterised in that：Step（1）
The specification of being sieved is 100 mesh, and temperature is 90~100 DEG C after heating water bath, and soaking time is 10~15min.
3. a kind of preparation method containing a variety of microbial inoculum biologic packing materials according to claim 1, it is characterised in that：Step（2）
Nitrococcus, saccharomycete and the strain of the bacillus subtilis mixing quality ratio is 1 ︰, 1 ︰ 3, and mixed bacteria cut-in quality is
The 5~8% of microbiological culture media quality, cultivation temperature are 30~35 DEG C, and incubation time is 18~20h.
4. a kind of preparation method containing a variety of microbial inoculum biologic packing materials according to claim 1, it is characterised in that：Step（3）
The mass fraction of the physiological saline be 0.9%, mixed bacteria culture inoculation quality be diffusion culture medium quality 8~
10%, speed of agitator is 300~350r/min, and cultivation temperature is 30~33 DEG C, under the conditions of gravity-flow ventilation incubation time for 16~
18h。
5. a kind of preparation method containing a variety of microbial inoculum biologic packing materials according to claim 1, it is characterised in that：Step（4）
Described is placed in raw material ball in the baking oven that temperature is 70~80 DEG C after drying, and temperature after heating is 400 DEG C, calcination time 30
~40min, calcination temperature are 1000~1100 DEG C, and roasting time is 2~3h.
6. a kind of preparation method containing a variety of microbial inoculum biologic packing materials according to claim 1, it is characterised in that：Step（5）
The bored exposure time is 4~5 days, staticly settles the time as 1~2h, and discharge upper strata waste water is the 1/4~1/ of pool inner water volume
5, into aeration tank modified Nano mixture is added in until solids content concn reaches 5~10g/L in aeration tank, during standing sedimentation
Between for 2~3 weeks, water content is 5~10% after pool bottom sludge drying, and pressing pressure is 7~8MPa.
7. a kind of preparation method containing a variety of microbial inoculum biologic packing materials according to claim 1, it is characterised in that：Step（6）
Filler matrix dip time in mixed bacterial liquid is 4~5 days, and animal farm wastewater solid content is 4~5%, and dipping is hung
The film time is 20~25 days.
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| CN201810301484.1A CN108264152A (en) | 2018-04-04 | 2018-04-04 | A kind of preparation method containing a variety of microbial inoculum biologic packing materials |
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| CN201810301484.1A Withdrawn CN108264152A (en) | 2018-04-04 | 2018-04-04 | A kind of preparation method containing a variety of microbial inoculum biologic packing materials |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109354209A (en) * | 2018-10-15 | 2019-02-19 | 武汉二航路桥特种工程有限责任公司 | A kind of modified mineral-microorganism coupling water-purifying material and preparation method thereof |
| CN110550818A (en) * | 2019-08-20 | 2019-12-10 | 生态环境部华南环境科学研究所 | Efficient biogas slurry desalting treatment process after fermentation of dairy cow breeding wastewater |
| CN111072154A (en) * | 2019-12-23 | 2020-04-28 | 安徽晟鑫农业科技发展有限公司 | Preparation method of sewage treatment agent by using microorganisms |
| CN113772809A (en) * | 2021-09-14 | 2021-12-10 | 同济大学 | Adsorption filler synergistic bioactivation permeable reaction column and preparation method and application thereof |
| CN114524504A (en) * | 2021-12-27 | 2022-05-24 | 山东坊能新动能科学研究院有限公司 | Biomass-loaded permeable reactive barrier material for underground water treatment and preparation method thereof |
| CN114804349A (en) * | 2022-04-24 | 2022-07-29 | 桂林电子科技大学 | Anaerobic ammonium oxidation sludge activator and preparation method thereof |
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2018
- 2018-04-04 CN CN201810301484.1A patent/CN108264152A/en not_active Withdrawn
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109354209A (en) * | 2018-10-15 | 2019-02-19 | 武汉二航路桥特种工程有限责任公司 | A kind of modified mineral-microorganism coupling water-purifying material and preparation method thereof |
| CN109354209B (en) * | 2018-10-15 | 2022-02-08 | 武汉二航路桥特种工程有限责任公司 | Modified mineral-microorganism coupling water purification material and preparation method thereof |
| CN110550818A (en) * | 2019-08-20 | 2019-12-10 | 生态环境部华南环境科学研究所 | Efficient biogas slurry desalting treatment process after fermentation of dairy cow breeding wastewater |
| CN110550818B (en) * | 2019-08-20 | 2021-12-07 | 生态环境部华南环境科学研究所 | Efficient biogas slurry desalting treatment process after fermentation of dairy cow breeding wastewater |
| CN111072154A (en) * | 2019-12-23 | 2020-04-28 | 安徽晟鑫农业科技发展有限公司 | Preparation method of sewage treatment agent by using microorganisms |
| CN113772809A (en) * | 2021-09-14 | 2021-12-10 | 同济大学 | Adsorption filler synergistic bioactivation permeable reaction column and preparation method and application thereof |
| CN114524504A (en) * | 2021-12-27 | 2022-05-24 | 山东坊能新动能科学研究院有限公司 | Biomass-loaded permeable reactive barrier material for underground water treatment and preparation method thereof |
| CN114804349A (en) * | 2022-04-24 | 2022-07-29 | 桂林电子科技大学 | Anaerobic ammonium oxidation sludge activator and preparation method thereof |
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Application publication date: 20180710 |