JP2006016386A - Microorganism-containing composition - Google Patents
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Abstract
Description
本発明は、土壌病害防除能を有する微生物含有組成物、その製造方法、微生物含有組成物を含む農園芸用資材、土壌改良用資材および肥料、堆肥の製造方法ならびに農作物栽培方法に関する。 The present invention relates to a microorganism-containing composition having a soil disease control ability, a method for producing the same, an agricultural and horticultural material containing the microorganism-containing composition, a soil improving material and fertilizer, a method for producing compost, and a method for cultivating crops.
土壌病害は土壌中に存在する植物病原菌による土壌伝染性の病害であり、特に連作障害の主要な原因となっている。現在、土壌病害の発生を抑制するために、臭化メチルやクロルピクリン等の化学農薬が多く利用されている。しかしながら、これによって土壌中の有益な微生物も死滅するため、土壌の肥沃さが失われる等の弊害が生じている。 Soil diseases are soil-borne diseases caused by phytopathogenic bacteria present in the soil, and are a major cause of continuous cropping disorders. Currently, chemical pesticides such as methyl bromide and chloropicrin are widely used to suppress the occurrence of soil diseases. However, since beneficial microorganisms in the soil are also killed by this, harmful effects such as loss of soil fertility occur.
これらの化学農薬に替わり、微生物の拮抗作用を利用した土壌改良剤や微生物資材等が提案されている。例えば、特開平６−１３５８１０号公報（特許文献１）には土壌病原菌に対して拮抗作用のある微生物の培養液を基材にスプレー噴霧する微生物資材の製造法が開示されている。また、特開平３−７７８０３号公報（特許文献２）には飢餓状態とした抗生物質生産菌をキャリアと混合して土壌に施用する土壌病害防止法が開示されている。 In place of these chemical pesticides, soil improvers, microbial materials, and the like using antagonism of microorganisms have been proposed. For example, Japanese Patent Laid-Open No. 6-135810 (Patent Document 1) discloses a method for producing a microbial material by spraying a culture solution of a microorganism having an antagonistic action against a soil pathogen on a substrate. Japanese Laid-Open Patent Publication No. 3-77803 (Patent Document 2) discloses a soil disease prevention method in which a starved antibiotic-producing bacterium is mixed with a carrier and applied to soil.
しかしながら、これまでの微生物資材では水分含量について十分に考慮されていないことが多く、流通過程において、または保存状態において菌数が減少したり、水分含量が高いためにカビ等の常在微生物に汚染される等、安定性に欠け、実際に土壌に施用した際に十分に有効と認められるだけの効果が得られないことがあった。
また、使用する際にも高濃度の菌体を含む薬剤を土壌に均一に施用するのが困難であること、十分量の菌数を土壌に施用しても定着性が悪く十分に有効な効果を示さないことといった課題があった。
さらに、堆肥を利用することにより土壌中の微生物相を改善し、土壌の肥沃さを向上することが行われている。
However, the moisture content of conventional microbial materials is often not sufficiently considered, and the number of bacteria decreases during the distribution process or in the storage state, or because of the high moisture content, contamination with resident microorganisms such as mold In some cases, the effect is not stable enough to be recognized as effective enough when actually applied to soil.
In addition, even when used, it is difficult to uniformly apply a drug containing a high concentration of bacterial cells to the soil, and even if a sufficient amount of bacteria is applied to the soil, it is poorly fixed and has a sufficiently effective effect There was a problem of not showing.
Furthermore, the use of compost improves the microflora in the soil and improves the fertility of the soil.
しかしながらこのような方法のみで、あるいはこれらの方法の組み合わせでも十分な効果は得られないことがあり、このような状況から、安定性が良好で、十分な効果がある微生物資材およびその施用方法が望まれていた。 However, a sufficient effect may not be obtained by such a method alone or by a combination of these methods. From such a situation, a microbial material having a good stability and a sufficient effect and a method for applying the same can be obtained. It was desired.
本発明は、安定性が良好で、十分な効果がある微生物資材の提供およびその施用方法の提供を課題の一つとする。 An object of the present invention is to provide a microbial material having good stability and sufficient effect and to provide a method for applying the microbial material.
本発明者らは、上記課題を解決するために鋭意研究を行った結果、バチルス・ズブチリスの培養液を有機物のキャリアと混合し、水分含量を３０質量％以下とすることにより、安定性が向上し、これを堆肥に代表される有機肥料の製造工程中に添加し、有機肥料に混和した形で土壌に施用することにより、土壌病害が発生することなく農作物の収量が向上することを見出し、本発明を完成するに至った。 As a result of diligent research to solve the above-mentioned problems, the inventors have improved stability by mixing a culture medium of Bacillus subtilis with an organic carrier to reduce the water content to 30% by mass or less. In addition, it is found that the yield of crops can be improved without causing soil diseases by adding this to the fertilizer manufacturing process represented by compost and applying it to the soil mixed with the organic fertilizer. The present invention has been completed.
すなわち本発明は以下の微生物含有組成物、微生物含有組成物の製造方法、微生物含有組成物を含む農園芸用資材、土壌改良用資材、肥料、堆肥の製造方法及び農作物栽培方法に関する。 That is, the present invention relates to the following microorganism-containing composition, method for producing the microorganism-containing composition, agricultural and horticultural materials containing the microorganism-containing composition, soil improving material, fertilizer, compost producing method, and crop cultivation method.
１．土壌病害性微生物に対し拮抗作用を示すバチルス・ズブチリス（Bacillus subtilis）の培養液を有機物のキャリアと混合し、乾燥工程を経ずに水分含量を３０質量％以下とした土壌病害防除能を有する微生物含有組成物。
２．粉末状または粒状である前記１に記載の微生物含有組成物。
３．バチルス・ズブチリスがバチルス・ズブチリス ＳＤ１４２（ＦＥＲＭ ＢＰ−８４２７）である前記１に記載の微生物含有組成物。
４．有機物のキャリアが乾燥醗酵粕、籾殻、米糠または麦糠である前記１に記載の微生物含有組成物。
５．混合後の水分含量が２０質量％以下である前記１乃至４のいずれか１項に記載の微生物含有組成物。
６．バチルス・ズブチリスの培養液を粉末状または粒状の有機物のキャリアと混合し、乾燥工程を経ずに水分含量が３０質量％以下の粉末状、または粒状にする微生物含有組成物の製造法。
７．バチルス・ズブチリスの培養液を有機物のキャリアと混合し、乾燥工程を経ずに水分含量を３０質量％以下とし、混合後、粉末状、または粒状とすることを特徴とする微生物含有組成物の製造法。
８．前記１乃至５のいずれか１項に記載の微生物含有組成物を含む農園芸用資材。
９．前記１乃至５のいずれか１項に記載の微生物含有組成物を含む土壌改良用資材。
１０．前記１乃至５のいずれか１項に記載の微生物含有組成物および有機肥料を含む肥料。
１１．有機肥料が堆肥である前記１０に記載の肥料。
１２．有機肥料が液肥である前記１０に記載の肥料。
１３．前記１乃至５のいずれか１項に記載の微生物含有組成物を堆肥製造工程中に添加することを特徴とする堆肥の製造方法。
１４．前記１０乃至１２のいずれか１項に記載の肥料を土壌に施用する農作物栽培方法。
1. A microorganism having the ability to control soil diseases by mixing a culture solution of Bacillus subtilis , which shows an antagonistic action against soil-borne microorganisms, with an organic carrier and having a water content of 30% by mass or less without passing through a drying step. Containing composition.
2. 2. The microorganism-containing composition according to 1 above, which is powdery or granular.
3. 2. The microorganism-containing composition according to 1 above, wherein the Bacillus subtilis is Bacillus subtilis SD142 (FERM BP-8427).
4). 2. The microorganism-containing composition according to 1 above, wherein the organic carrier is dry fermented rice cake, rice husk, rice bran or wheat straw.
5. The microorganism-containing composition according to any one of 1 to 4, wherein the water content after mixing is 20% by mass or less.
6). A method for producing a microorganism-containing composition, wherein a culture solution of Bacillus subtilis is mixed with a powdery or granular organic carrier to form a powdery or granular material having a water content of 30% by mass or less without passing through a drying step.
7). Production of a microorganism-containing composition, wherein a culture solution of Bacillus subtilis is mixed with an organic carrier, the moisture content is reduced to 30% by mass or less without passing through a drying step, and the mixture is powdered or granulated after mixing. Law.
8). An agricultural and horticultural material comprising the microorganism-containing composition according to any one of 1 to 5 above.
9. The material for soil improvement containing the microorganisms containing composition of any one of said 1 thru | or 5.
10. A fertilizer comprising the microorganism-containing composition according to any one of 1 to 5 and an organic fertilizer.
11. 11. The fertilizer according to 10 above, wherein the organic fertilizer is compost.
12 11. The fertilizer according to 10 above, wherein the organic fertilizer is liquid fertilizer.
13. 6. A method for producing compost, wherein the microorganism-containing composition according to any one of 1 to 5 is added during a compost production process.
14 A method for cultivating crops, wherein the fertilizer according to any one of 10 to 12 is applied to soil.
以下、本発明について詳細に説明する。
本発明に用いられるバチルス・ズブチリス（Bacillus subtilis）は、土壌病害性微生物に対し拮抗作用を示すものであればいかなるものでもよいが、イツリンを産生するものが好ましく、中でも特願２００３−３３０６２３に記載のバチルス・ズブチリス ＳＤ１４２（ＦＥＲＭ ＢＰ−８４２７）が特に好ましく用いられる。バチルス・ズブチリス ＳＤ１４２は、２００２年９月２４日に茨城県つくば市東１丁目１番地１ 中央第６（郵便番号３０５−８５６６）の独立行政法人産業技術総合研究所に寄託され（受託番号 ＦＥＲＭ Ｐ−１９０３２）、２００３年７月１０日に国際寄託に移管されている（国際受託番号 ＦＥＲＭ ＢＰ−８４２７）。イツリンは特開昭５９−２１２４１６、特許１４７１８４３に記載されてように土壌病害性微生物に対し抗菌効果がある物質として知られている。
Hereinafter, the present invention will be described in detail.
The Bacillus subtilis used in the present invention may be any as long as it exhibits an antagonistic action against soil-damaging microorganisms, but is preferably one that produces iturin, and particularly described in Japanese Patent Application No. 2003-330623. Bacillus subtilis SD142 (FERM BP-8427) is particularly preferably used. Bacillus subtilis SD142 was deposited on September 24, 2002 at the National Institute of Advanced Industrial Science and Technology (AIST), 1-6 Higashi 1-chome, Tsukuba, Ibaraki, Japan (zip code 305-8656). 19032), and transferred to an international deposit on July 10, 2003 (international deposit number FERM BP-8427). Iturin is known as a substance having an antibacterial effect against soil-damaging microorganisms as described in JP-A No. 59-212416 and Japanese Patent No. 1471843.
バチルス・ズブチリス（Bacillus subtilis）を培養する方法は公知の方法を用いることができる。すなわち、ペプトン、肉エキス、大豆粉などの窒素源、グルコース、水飴、デキストリンなどの炭素源、無機塩類、酵母エキス、等の一般的な原料を用いた培地で培養することができる。なかでも窒素源として大豆粉、炭素源としてグルコースまたは水飴が好ましく用いられる。 As a method for culturing Bacillus subtilis , a known method can be used. That is, it can be cultured in a medium using a general raw material such as nitrogen sources such as peptone, meat extract, and soybean powder, carbon sources such as glucose, starch syrup, and dextrin, inorganic salts, and yeast extract. Of these, soybean powder is preferably used as the nitrogen source, and glucose or chickenpox is preferably used as the carbon source.
このようにして得られるバチルス・ズブチリスの培養液をキャリアと混合する。キャリアとしては、ゼオライトや珪藻土等の多孔質の無機物を用いると堆肥等の有機肥料に添加した際に分解されず、菌が吸着されたままとなり、土壌中で有効に作用しないため、有機物のキャリアであることが必要である。有機物のキャリアは堆肥の製造過程で分解されるため、菌が堆肥中に均一に分布し、土壌に施用した際に有効に作用する。
有機物のキャリアは堆肥の製造過程で分解されるため、菌が堆肥中に均一に分布し、土壌に施用した際に有効に作用する。
The culture solution of Bacillus subtilis thus obtained is mixed with a carrier. If a porous inorganic material such as zeolite or diatomaceous earth is used as the carrier, it will not be decomposed when added to organic fertilizers such as compost and will remain adsorbed and will not act effectively in the soil. It is necessary to be. Since the organic carrier is decomposed during the compost production process, the fungus is distributed uniformly in the compost and works effectively when applied to the soil.
Since the organic carrier is decomposed during the compost production process, the fungus is distributed uniformly in the compost and works effectively when applied to the soil.
バチルス・ズブチリスの培養液と混合する有機物のキャリアとしては、稲ワラ、モミガラ、ピートモス、米糠、麦糠、畜糞、鶏糞、おが屑、落ち葉、菜種粕、バガス、おから、フスマ粕、乾燥ビール粕、乾燥清酒粕、乾燥焼酎粕等が挙げられる。堆肥中で分解されやすい乾燥ビール粕、乾燥清酒粕、乾燥焼酎粕等の乾燥醗酵粕、米糠、麦糠が好ましく使用できる。 Organic carriers to be mixed with Bacillus subtilis broth include rice straw, rice straw, peat moss, rice bran, wheat straw, livestock manure, chicken manure, sawdust, fallen leaves, rapeseed rice, bagasse, okara, bran rice, dried beer rice cake, Examples include dried sake lees and dried shochu. Dried beer lees, dry sake lees, dry fermented lees such as dried shochu, rice bran, and wheat straw that are easily decomposed in compost can be preferably used.
また、本発明の微生物含有組成物は粉末状または粒状のものが施用に好都合であるため有機物のキャリアとしては予め粉末状または粒状としたものを使用することが好ましいが、粉末状または粒状でないキャリアを使用し、混合物とした後に粉末あるいは粒状にしても良い。 In addition, since the microorganism-containing composition of the present invention is preferably in the form of powder or granules, it is preferable to use a powder or granule as the organic carrier in advance, but it is not a powder or granule carrier. May be used to form a mixture and then be powdered or granulated.
この際、培養液とキャリアを、仕上がった微生物含有組成物の水分含量が３０質量％以下になるように混合することが好ましく、２０質量％以下になるように混合することがより好ましい。 Under the present circumstances, it is preferable to mix a culture solution and a carrier so that the moisture content of the finished microorganisms-containing composition may be 30 mass% or less, and it is more preferable to mix so that it may become 20 mass% or less.
水分含量が高いときは、キャリアとして乾いた材料好ましくは乾いた有機材料、より好ましくは乾いたモミガラ、おが屑等を必要量使用して混合することにより、仕上がった微生物含有組成物の水分含量を好ましくは３０質量％以下、より好ましくは２０質量％以下になるように容易に調整することができる。 When the moisture content is high, the moisture content of the finished microorganism-containing composition is preferably adjusted by mixing the dry material as a carrier, preferably a dry organic material, more preferably dry rice crackers, sawdust, etc. Can be easily adjusted to 30% by mass or less, more preferably 20% by mass or less.
上記のようにして得られる培養液とキャリアの混合物は乾燥工程を経ないため、乾燥工程による菌の死滅がなく菌の生存率を高く維持することができ、保存安定性に優れており、高い性能を有する微生物含有組成物とすることができる。 Since the mixture of the culture solution and the carrier obtained as described above does not go through a drying process, it can maintain a high survival rate without killing the bacteria by the drying process, has excellent storage stability, and high It can be set as the microorganism-containing composition which has performance.
このようにして得られる本発明の微生物含有組成物は、これ自体で農園芸用資材または土壌改良用資材として使用することも可能であるが、これを添加した有機肥料を製造した上で使用すると土壌に容易に均一に施用できるため、極めて効果的である。 The microorganism-containing composition of the present invention thus obtained can be used as an agricultural or horticultural material or a soil improvement material by itself, but when used after producing an organic fertilizer to which this is added. It is very effective because it can be applied to soil easily and uniformly.
有機肥料が堆肥の場合、堆肥製造工程中、例えば一次堆積槽で本発明の微生物含有組成物を添加する方法、あるいは二次撹拌槽で添加する方法のいずれも可能であるが、二次撹拌槽で添加することが好ましい。本発明の微生物含有組成物の添加量は、堆肥原料当たり質量比で１／10000〜１／１００程度が好ましい。このようにして得られる肥料を土壌１ｍ2に対して通常0.1〜１０ｋｇ施用する。 When the organic fertilizer is compost, any of the method of adding the microorganism-containing composition of the present invention in the compost production process, for example, in the primary deposition tank, or the method of adding in the secondary stirring tank is possible. It is preferable to add at. The addition amount of the microorganism-containing composition of the present invention is preferably about 1/10000 to 1/100 in terms of mass ratio per compost raw material. The fertilizer thus obtained is usually applied in an amount of 0.1 to 10 kg per 1 m 2 of soil.
以下、実施例により本発明を具体的に説明するが、本発明はこれらの実施例によって何ら制限されるものではない。 EXAMPLES Hereinafter, although an Example demonstrates this invention concretely, this invention is not restrict | limited at all by these Examples.
実施例１
バチルス・ズブチリスＳＤ１４２を下記組成ＡのＬ培地に２％寒天を加えたＬ平板培地に画線し、３５℃で一晩生育させた。そこからＬ培地３Ｌを添加した５Ｌ容培養槽に植菌し、３５℃、６００ｒｐｍで６時間培養した。さらにＬ培地１５０Ｌを添加した２００Ｌ容培養槽に植菌し、３５℃、４００ｒｐｍで６時間培養した。次に下記組成Ｂの培地3000ｋｇを添加した5000Ｌ容培養槽に植菌し、３０℃、１８０ｒｐｍで７０時間培養を行い、バチルス・ズブチリスＳＤ１４２の培養液を得た。
Example 1
Bacillus subtilis SD142 was streaked on an L plate medium in which 2% agar was added to an L medium having the following composition A, and grown overnight at 35 ° C. From there, it was inoculated into a 5 L culture tank to which 3 L of L medium was added, and cultured at 35 ° C. and 600 rpm for 6 hours. Furthermore, it inoculated in a 200L culture tank which added L culture medium 150L, and culture | cultivated at 35 degreeC and 400 rpm for 6 hours. Next, it was inoculated into a 5000 L culture tank to which 3000 kg of the medium having the following composition B was added, and cultured at 30 ° C. and 180 rpm for 70 hours to obtain a culture solution of Bacillus subtilis SD142.
組成Ａ（Ｌ培地） （質量％）
ペプトン １
酵母エキス 0.5
塩化ナトリウム 0.5
水 残部
Composition A (L medium) (mass%)
Peptone 1
Yeast extract 0.5
Sodium chloride 0.5
Water balance
組成Ｂ （質量％）
大豆粉 ９
Ｋ2ＨＰＯ4 0.5
ＭｇＳＯ4・７Ｈ2Ｏ 0.05
ＦｅＳＯ4・７Ｈ2Ｏ 0.0025
ＭｎＳＯ4・５Ｈ2Ｏ 0.0025
ＣａＣｌ2 0.1
マルトース １７
水 残部
Composition B (mass%)
Soy flour 9
K 2 HPO 4 0.5
MgSO 4 · 7H 2 O 0.05
FeSO 4 · 7H 2 O 0.0025
MnSO 4 · 5H 2 O 0.0025
CaCl 2 0.1
Maltose 17
Water balance
得られた培養液（水分含量９０％）に対し、乾燥ビール粕（水分含量１３％）を１：１０の質量比で添加し、スクリューミキサーで撹拌混合し、水分含量２０％の微生物含有組成物を得た。 To the obtained culture broth (water content 90%), dry beer koji (water content 13%) is added at a mass ratio of 1:10, and stirred and mixed with a screw mixer, and a microorganism-containing composition having a water content of 20%. Got.
実施例２
実施例１のうち、乾燥ビール粕の替わりに麦糠（水分含量１３％）を用いて同様に行い、水分含量２０％の微生物含有組成物を得た。
Example 2
In Example 1, it was carried out in the same manner using wheat straw (water content 13%) instead of dry beer cake, and a microorganism-containing composition having a water content of 20% was obtained.
比較例１
培養液（水分含量９０％）に対し、乾燥ビール粕（水分含量１３％）と水を１：４：２の質量比で添加し、スクリューミキサーで撹拌混合し、減圧乾燥機にて１５０℃で２時間乾燥し、水分含量４％の微生物含有組成物を得た。
Comparative Example 1
To the culture solution (water content 90%), dry beer cake (water content 13%) and water are added at a mass ratio of 1: 4: 2, stirred and mixed with a screw mixer, and 150 ° C. with a vacuum dryer. It was dried for 2 hours to obtain a microorganism-containing composition having a water content of 4%.
比較例２
培養液（水分含量９０％）に対し、乾燥ビール粕（水分含量１３％）と水を１：４：２の質量比で添加し、スクリューミキサーで撹拌混合し、水分含量４９％の微生物含有組成物を得た。
Comparative Example 2
Dried beer cake (water content 13%) and water are added at a mass ratio of 1: 4: 2 to the culture solution (water content 90%), mixed by stirring with a screw mixer, and a microorganism-containing composition with a water content of 49% I got a thing.
実施例１、２および比較例１、２の組成物を室温で保存し、経時的に組成物中の生菌数を測定し、菌体の生存率を算出した。生菌数の測定は、組成物を滅菌水で適宜希釈したものをＬ平版培地に塗布し、３５℃で一晩培養して生育したコロニー数を計測することにより求めた。結果を表１に示す。生存率は実施例１の０日の生菌数を１００％としたときの相対値で示した。 The compositions of Examples 1 and 2 and Comparative Examples 1 and 2 were stored at room temperature, the number of viable bacteria in the composition was measured over time, and the survival rate of the cells was calculated. The number of viable bacteria was determined by applying a composition obtained by appropriately diluting the composition with sterilized water to an L lithographic medium, and culturing it overnight at 35 ° C. to count the number of colonies grown. The results are shown in Table 1. The survival rate was expressed as a relative value when the number of viable bacteria on day 0 in Example 1 was taken as 100%.
実施例１、２および比較例１、２の組成物を各々堆肥製造工程の二次醗酵槽の入口に原料とともに添加して製造した堆肥を得た（実施例３、４、比較例３、４）。組成物の添加量は堆肥に対し質量比で３／１００とした。また、組成物を添加せずに製造した堆肥を得た（比較例５）。 Composts produced by adding the compositions of Examples 1 and 2 and Comparative Examples 1 and 2 together with the raw materials to the inlet of the secondary fermenter in the compost production process were obtained (Examples 3 and 4, Comparative Examples 3 and 4). ). The addition amount of the composition was 3/100 in terms of mass ratio with respect to compost. Moreover, the compost produced without adding the composition was obtained (Comparative Example 5).
実施例３、４および比較例３〜５の堆肥を室温で保存し、経時的に堆肥中の組成物由来の生菌数を測定し、菌体の生存率を算出した。組成物由来の生菌数の測定は、組成物を滅菌水で適宜希釈した後８０℃で３０分加熱したものを、２％羊血液を添加したＬ平版培地に塗布し、３５℃で一晩培養して生育したコロニーの周囲にクリアゾーンを形成するコロニー数を計測することにより求めた。結果を表２に示す。生存率は実施例３の０日の生菌数を１００％としたときの相対値で示した。 The composts of Examples 3 and 4 and Comparative Examples 3 to 5 were stored at room temperature, the number of viable bacteria derived from the composition in the compost was measured over time, and the survival rate of the bacterial cells was calculated. The number of viable bacteria derived from the composition was measured by appropriately diluting the composition with sterilized water and heating at 80 ° C. for 30 minutes on an L lithographic medium supplemented with 2% sheep blood, and overnight at 35 ° C. It calculated | required by measuring the number of colonies which form a clear zone around the colony grown by culture | cultivation. The results are shown in Table 2. The survival rate was expressed as a relative value when the number of viable bacteria on day 0 in Example 3 was taken as 100%.
実施例３および比較例５の堆肥を土壌１ｍ2当たり４ｋｇ施用して各種農作物の収量を調べた。収量は比較例５（微生物含有組成物を添加しない堆肥）の収量を１００としたときの相対値で示した。また、それぞれの栽培における病害発生の有無を示した。結果を表３に示す。 The yield of various agricultural crops was examined by applying 4 kg of the compost of Example 3 and Comparative Example 5 per 1 m 2 of soil. The yield was shown as a relative value when the yield of Comparative Example 5 (compost without addition of the microorganism-containing composition) was taken as 100. Moreover, the presence or absence of the disease occurrence in each cultivation was shown. The results are shown in Table 3.
Claims (14)
A crop cultivation method in which the fertilizer according to any one of claims 10 to 12 is applied to soil.
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2007138781A1 (en) * | 2006-05-25 | 2007-12-06 | Saihatsu Kou | Antiviral agent and antibacterial agent |
| JP2008029315A (en) * | 2006-07-27 | 2008-02-14 | Takashi Tsurumizu | Separate culture method (including mutational phase) of vesicular arbuscular mycorrhiza and method for producing viable bacterium material of cultured cell (including mutational phase) |
| JP2008178841A (en) * | 2007-01-26 | 2008-08-07 | Tokiwa Reitou Shokuhin Kk | Method for treating yu (tofu whey) and liquid fertilizer and deodorization liquid produced by this method |
| JP2009091523A (en) * | 2007-10-12 | 2009-04-30 | Three S Kk | Manufacturing method of soil activator |
| JP2012050461A (en) * | 2011-12-02 | 2012-03-15 | Takashi Tsurumizu | Method for culturing va mycorrhizal fungus |
| CN102757277A (en) * | 2012-07-31 | 2012-10-31 | 长春华御实业集团有限公司 | Paddy biofertilizer, preparation method thereof and bacillus subtilis |
| WO2015020080A1 (en) | 2013-08-09 | 2015-02-12 | カルピス株式会社 | Method for cultivating crops using bacterium belonging to genus bacillus |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH06135810A (en) * | 1992-10-27 | 1994-05-17 | Takasaki Kasei Kk | Production of microbial material capable of suppressing soil disease and injury |
| JPH0892022A (en) * | 1994-09-29 | 1996-04-09 | Asahi Chem Ind Co Ltd | Controlling method for snow mold injury and microbial material for it |
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- 2005-05-31 JP JP2005159669A patent/JP2006016386A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH06135810A (en) * | 1992-10-27 | 1994-05-17 | Takasaki Kasei Kk | Production of microbial material capable of suppressing soil disease and injury |
| JPH0892022A (en) * | 1994-09-29 | 1996-04-09 | Asahi Chem Ind Co Ltd | Controlling method for snow mold injury and microbial material for it |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2007138781A1 (en) * | 2006-05-25 | 2007-12-06 | Saihatsu Kou | Antiviral agent and antibacterial agent |
| JP2008029315A (en) * | 2006-07-27 | 2008-02-14 | Takashi Tsurumizu | Separate culture method (including mutational phase) of vesicular arbuscular mycorrhiza and method for producing viable bacterium material of cultured cell (including mutational phase) |
| JP2008178841A (en) * | 2007-01-26 | 2008-08-07 | Tokiwa Reitou Shokuhin Kk | Method for treating yu (tofu whey) and liquid fertilizer and deodorization liquid produced by this method |
| JP2009091523A (en) * | 2007-10-12 | 2009-04-30 | Three S Kk | Manufacturing method of soil activator |
| JP2012050461A (en) * | 2011-12-02 | 2012-03-15 | Takashi Tsurumizu | Method for culturing va mycorrhizal fungus |
| CN102757277A (en) * | 2012-07-31 | 2012-10-31 | 长春华御实业集团有限公司 | Paddy biofertilizer, preparation method thereof and bacillus subtilis |
| WO2015020080A1 (en) | 2013-08-09 | 2015-02-12 | カルピス株式会社 | Method for cultivating crops using bacterium belonging to genus bacillus |
| JPWO2015020080A1 (en) * | 2013-08-09 | 2017-03-02 | アサヒカルピスウェルネス株式会社 | Cultivation method of crops using Bacillus bacteria |
| US10899677B2 (en) | 2013-08-09 | 2021-01-26 | Asahi Biocycle Co., Ltd. | Method for cultivating crops using bacterium belonging to genus Bacillus |
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