JP2006016385A - Microorganism-containing composition - Google Patents

Microorganism-containing composition Download PDF

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JP2006016385A
JP2006016385A JP2005159668A JP2005159668A JP2006016385A JP 2006016385 A JP2006016385 A JP 2006016385A JP 2005159668 A JP2005159668 A JP 2005159668A JP 2005159668 A JP2005159668 A JP 2005159668A JP 2006016385 A JP2006016385 A JP 2006016385A
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microorganism
containing composition
soil
fertilizer
bacillus subtilis
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Tadashi Yoneda
正 米田
Eiji Ogata
英二 小方
Katsuharu Nakano
勝春 中野
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Showa Denko KK
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Showa Denko KK
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Abstract

<P>PROBLEM TO BE SOLVED: To provide a microorganismal material with microorganisms preserved stably in preserved conditions including distribution process, having sufficient effect in its application, and to provide a method for applying the material. <P>SOLUTION: A microorganism-containing composition having the ability to control soil disease injuries is provided, which is obtained by mixing preferably a powdery or granular organic matter carrier with a culture liquid of Bacillus subtilis exhibiting antagonistic activity on soil-pathogenic microbes followed by evaporation under reduced pressure at ≤100°C to effect 30 mass% moisture content. An agricultural/horticultural material containing the above composition is also provided. By adding this composition during the process of producing an organic fertilizer represented by compost and applying this composition in the form of being admixed with the organic fertilizer, farm product yield is improved without onset of soil disease injuries. <P>COPYRIGHT: (C)2006,JPO&NCIPI

Description

本発明は、土壌病害防除能を有する微生物含有組成物、その製造方法、微生物含有組成物を含む農園芸用資材、土壌改良用資材および肥料、堆肥の製造方法ならびに農作物栽培方法に関する。   The present invention relates to a microorganism-containing composition having a soil disease control ability, a method for producing the same, an agricultural and horticultural material containing the microorganism-containing composition, a soil improving material and fertilizer, a method for producing compost, and a method for cultivating crops.

土壌病害は土壌中に存在する植物病原菌による土壌伝染性の病害であり、特に連作障害の主要な原因となっている。現在、土壌病害の発生を抑制するために、臭化メチルやクロルピクリン等の化学農薬が多く利用されている。しかしながら、これによって土壌中の有益な微生物も死滅するため、土壌の肥沃さが失われる等の弊害が生じている。   Soil diseases are soil-borne diseases caused by phytopathogenic bacteria present in the soil, and are a major cause of continuous cropping disorders. Currently, chemical pesticides such as methyl bromide and chloropicrin are widely used to suppress the occurrence of soil diseases. However, since beneficial microorganisms in the soil are also killed by this, harmful effects such as loss of soil fertility occur.

これらの化学農薬に替わり、微生物の拮抗作用を利用した土壌改良剤や微生物資材等が提案されている。例えば、特開平6−135810号公報(特許文献1)には土壌病原菌に対して拮抗作用のある微生物の培養液を基材にスプレー噴霧する微生物資材の製造法が開示されている。また、特開平3−77803号公報(特許文献2)には飢餓状態とした抗生物質生産菌をキャリアと混合して土壌に施用する土壌病害防止法が開示されている。   In place of these chemical pesticides, soil improvers, microbial materials, and the like using antagonism of microorganisms have been proposed. For example, Japanese Patent Laid-Open No. 6-135810 (Patent Document 1) discloses a method for producing a microbial material by spraying a culture solution of a microorganism having an antagonistic action against a soil pathogen on a substrate. Japanese Laid-Open Patent Publication No. 3-77803 (Patent Document 2) discloses a soil disease prevention method in which a starved antibiotic-producing bacterium is mixed with a carrier and applied to soil.

しかしながら、これまでの微生物資材では水分含量について十分に考慮されていないことが多く、流通過程において、または保存状態において菌数が減少したり、水分含量が高いためにカビ等の常在微生物に汚染される等、安定性に欠け、実際に土壌に施用した際に十分に有効と認められるだけの効果が得られないことがあった。
また、使用する際にも高濃度の菌体を含む薬剤を土壌に均一に施用するのが困難であること、十分量の菌数を土壌に施用しても定着性が悪く十分に有効な効果を示さないことといった課題があった。
さらに、堆肥を利用することにより土壌中の微生物相を改善し、土壌の肥沃さを向上することが行われている。
However, the moisture content of conventional microbial materials is often not sufficiently considered, and the number of bacteria decreases during the distribution process or in the storage state, or contamination with resident microorganisms such as mold due to the high moisture content. In some cases, the effect is not stable enough to be recognized as effective enough when actually applied to soil.
In addition, even when used, it is difficult to uniformly apply a drug containing a high concentration of bacterial cells to the soil, and even if a sufficient amount of bacteria is applied to the soil, the fixability is poor and a sufficiently effective effect There was a problem of not showing.
Furthermore, the use of compost improves the microflora in the soil and improves the fertility of the soil.

しかしながらこのような方法のみで、あるいはこれらの方法の組み合わせでも十分な効果は得られないことがあり、このような状況から、安定性が良好で、十分な効果がある微生物資材およびその施用方法が望まれていた。   However, a sufficient effect may not be obtained by such a method alone or by a combination of these methods. From such a situation, a microbial material having a good stability and a sufficient effect and a method for applying the same can be obtained. It was desired.

特開平6−135810号公報JP-A-6-135810 特開平3−77803号公報JP-A-3-77803

本発明は、安定性が良好で、十分な効果がある微生物資材の提供およびその施用方法の提供を課題の一つとする。   An object of the present invention is to provide a microbial material having good stability and sufficient effect and to provide a method for applying the microbial material.

本発明者らは、上記課題を解決するために鋭意研究を行った結果、バチルス・ズブチリスの培養液を有機物のキャリアと混合し、100℃以下の温度で減圧乾燥することにより、安定性が向上し、これを堆肥に代表される有機肥料の製造工程中に添加し、有機肥料に混和した形で土壌に施用することにより、土壌病害が発生することなく農作物の収量が向上することを見出し、本発明を完成するに至った。   As a result of diligent research to solve the above problems, the inventors have improved stability by mixing a culture medium of Bacillus subtilis with an organic carrier and drying under reduced pressure at a temperature of 100 ° C. or lower. In addition, it is found that the yield of crops can be improved without causing soil diseases by adding this to the fertilizer manufacturing process represented by compost and applying it to the soil mixed with the organic fertilizer. The present invention has been completed.

すなわち本発明は以下の微生物含有組成物、微生物含有組成物の製造方法、微生物含有組成物を含む農園芸用資材、土壌改良用資材、肥料、堆肥の製造方法及び農作物栽培方法に関する。   That is, the present invention relates to the following microorganism-containing composition, method for producing the microorganism-containing composition, agricultural and horticultural materials containing the microorganism-containing composition, soil improving material, fertilizer, compost producing method, and crop cultivation method.

1.土壌病害性微生物に対し拮抗作用を示すバチルス・ズブチリス(Bacillus subtilis)の培養液を有機物のキャリアと混合し、100℃以下の温度で減圧乾燥してなる土壌病害防除能を有する微生物含有組成物。
2.粉末状または粒状である前記1に記載の微生物含有組成物。
3.バチルス・ズブチリスがバチルス・ズブチリス SD142(FERM BP−8427)である前記1に記載の微生物含有組成物。
4.有機物のキャリアが乾燥醗酵粕または麦糠である前記1に記載の微生物含有組成物。
5.乾燥後の水分含量が30質量%以下である前記1乃至4のいずれか1項に記載の微生物含有組成物。
6.バチルス・ズブチリスの培養液を粉末状または粒状の有機物キャリアと混合し、100℃以下の温度で減圧乾燥することを特徴とする土壌病害防除能を有する微生物含有組成物の製造方法。
7.バチルス・ズブチリスの培養液を有機物キャリアと混合し、100℃以下の温度で減圧乾燥した後、粉末状または粒状とすることを特徴とする土壌病害防除能を有する微生物含有組成物の製造方法。
8.前記1乃至5のいずれか1項に記載の微生物含有組成物を含む農園芸用資材。
9.前記1乃至5のいずれか1項に記載の微生物含有組成物を含む土壌改良用資材。
10.前記1乃至5のいずれか1項に記載の微生物含有組成物および有機肥料を含む肥料。
11.有機肥料が堆肥である前記10に記載の肥料。
12.有機肥料が液肥である前記10に記載の肥料。
13.前記1乃至5のいずれか1項に記載の微生物含有組成物を堆肥製造工程中に添加することを特徴とする堆肥の製造方法。
14.前記10乃至12のいずれか1項に記載の肥料を土壌に施用する農作物栽培方法。
1. A microorganism-containing composition having a soil disease control ability obtained by mixing a culture solution of Bacillus subtilis having an antagonistic action against a soil disease-causing microorganism with an organic carrier and drying under reduced pressure at a temperature of 100 ° C. or lower.
2. 2. The microorganism-containing composition according to 1 above, which is powdery or granular.
3. 2. The microorganism-containing composition according to 1 above, wherein the Bacillus subtilis is Bacillus subtilis SD142 (FERM BP-8427).
4). 2. The microorganism-containing composition according to 1 above, wherein the organic carrier is dried fermented koji or wheat straw.
5. 5. The microorganism-containing composition according to any one of 1 to 4 above, wherein the moisture content after drying is 30% by mass or less.
6). A method for producing a microorganism-containing composition having a soil disease control ability, wherein a culture solution of Bacillus subtilis is mixed with a powdered or granular organic carrier and dried under reduced pressure at a temperature of 100 ° C or lower.
7). A method for producing a microorganism-containing composition having a soil disease control ability, wherein a culture solution of Bacillus subtilis is mixed with an organic carrier, dried under reduced pressure at a temperature of 100 ° C. or lower, and then powdered or granular.
8). An agricultural and horticultural material comprising the microorganism-containing composition according to any one of 1 to 5 above.
9. The material for soil improvement containing the microorganisms containing composition of any one of said 1 thru | or 5.
10. A fertilizer comprising the microorganism-containing composition according to any one of 1 to 5 and an organic fertilizer.
11. 11. The fertilizer according to 10 above, wherein the organic fertilizer is compost.
12 11. The fertilizer according to 10 above, wherein the organic fertilizer is liquid fertilizer.
13. 6. A method for producing compost, wherein the microorganism-containing composition according to any one of 1 to 5 is added during a compost production process.
14 A method for cultivating crops, wherein the fertilizer according to any one of 10 to 12 is applied to soil.

以下、本発明について詳細に説明する。
本発明に用いられるバチルス・ズブチリス(Bacillus subtilis)は、土壌病害性微生物に対し拮抗作用を示すものであればいかなるものでもよいが、イツリンを産生するものが好ましく、中でも特願2003−330623に記載のバチルス・ズブチリス SD142(FERM BP−8427)が特に好ましく用いられる。バチルス・ズブチリス SD142は、2002年9月24日に茨城県つくば市東1丁目1番地1 中央第6(郵便番号305−8566)の独立行政法人産業技術総合研究所に寄託され(受託番号 FERM P−19032)、2003年7月10日に国際寄託に移管されている(国際受託番号 FERM BP−8427)。イツリンは特開昭59−212416、特許1471843に記載されてように土壌病害性微生物に対し抗菌効果がある物質として知られている。
Hereinafter, the present invention will be described in detail.
The Bacillus subtilis used in the present invention may be any as long as it exhibits an antagonistic action against soil-damaging microorganisms, but is preferably one that produces iturin, and particularly described in Japanese Patent Application No. 2003-330623. Bacillus subtilis SD142 (FERM BP-8427) is particularly preferably used. Bacillus subtilis SD142 was deposited on September 24, 2002 at the National Institute of Advanced Industrial Science and Technology (AIST), 1-6 Higashi 1-chome, Tsukuba, Ibaraki, Japan (postal code 305-8565). 19032), and transferred to an international deposit on July 10, 2003 (international deposit number FERM BP-8427). Iturin is known as a substance having an antibacterial effect against soil-damaging microorganisms as described in JP-A No. 59-212416 and Japanese Patent No. 1471843.

バチルス・ズブチリス(Bacillus subtilis)を培養する方法は公知の方法を用いることができる。すなわち、ペプトン、肉エキス、大豆粉などの窒素源、グルコース、水飴、デキストリンなどの炭素源、無機塩類、酵母エキス、等の一般的な原料を用いた培地で培養することができる。なかでも窒素源として大豆粉、炭素源としてグルコースまたは水飴が好ましく用いられる。 As a method for culturing Bacillus subtilis , a known method can be used. That is, it can be cultured in a medium using nitrogen sources such as peptone, meat extract, soybean powder, etc., carbon sources such as glucose, starch syrup, and dextrin, inorganic salts, yeast extract and the like. Of these, soybean powder is preferably used as the nitrogen source, and glucose or chickenpox is preferably used as the carbon source.

このようにして得られるバチルス・ズブチリスの培養液をキャリアと混合する。キャリアとしては、ゼオライトや珪藻土等の多孔質の無機物を用いると堆肥等の有機肥料に添加した際に分解されず、菌が吸着されたままとなり、土壌中で有効に作用しないため、有機物のキャリアであることが必要である。有機物のキャリアは堆肥の製造過程で分解されるため、菌が堆肥中に均一に分布し、土壌に施用した際に有効に作用する。
有機物のキャリアは堆肥の製造過程で分解されるため、菌が堆肥中に均一に分布し、土壌に施用した際に有効に作用する。
The culture solution of Bacillus subtilis thus obtained is mixed with a carrier. If a porous inorganic material such as zeolite or diatomaceous earth is used as the carrier, it will not be decomposed when added to organic fertilizers such as compost, and the bacteria will remain adsorbed and will not act effectively in the soil. It is necessary to be. Since organic carriers are decomposed during the compost production process, the fungi are evenly distributed in the compost and work effectively when applied to the soil.
Since organic carriers are decomposed during the compost production process, the fungi are evenly distributed in the compost and work effectively when applied to the soil.

バチルス・ズブチリスの培養液と混合する有機物のキャリアとしては、稲ワラ、モミガラ、ピートモス、米糠、麦糠、畜糞、鶏糞、おが屑、落ち葉、菜種粕、バガス、おから、フスマ粕、乾燥ビール粕、乾燥清酒粕、乾燥焼酎粕等が挙げられる。堆肥中で分解されやすい乾燥ビール粕、乾燥清酒粕、乾燥焼酎粕等の乾燥醗酵粕が好ましく使用できる。   Organic carriers to be mixed with Bacillus subtilis broth include rice straw, rice straw, peat moss, rice bran, wheat straw, animal manure, chicken manure, sawdust, fallen leaves, rapeseed rice, bagasse, okara, bran rice, dried beer rice cake, Examples include dried sake lees and dried shochu. Dry fermented koji such as dried beer koji, dried sake koji, and dried shochu that are easily decomposed in compost can be preferably used.

また、本発明の微生物含有組成物は粉末状または粒状のものが施用に好都合であるため有機物のキャリアとしては予め粉末状または粒状としたものを使用することが好ましいが、粉末状または粒状でないキャリアを使用し、混合物を100℃以下の温度で減圧乾燥した後に粉末あるいは粒状にしても良い。   In addition, since the microorganism-containing composition of the present invention is preferably in the form of powder or granules, it is preferable to use a powder or granule as the organic carrier in advance, but it is not a powder or granule carrier. And the mixture may be dried in vacuo at a temperature of 100 ° C. or lower and then powdered or granulated.

バチルス・ズブチリスの培養液と有機物のキャリアの混合比は、質量比で1:10〜1:1、水分含量として30〜60質量%とするのが好ましく、必要に応じて水を添加してもよい。水分含量が30%に満たないと均一に混合することが困難であり、60%を超えると流動性が悪くなるため好ましくない。   The mixing ratio of the culture medium of Bacillus subtilis and the organic carrier is preferably 1:10 to 1: 1 by mass ratio and 30 to 60% by mass as the water content, and even if water is added if necessary. Good. If the water content is less than 30%, it is difficult to mix uniformly, and if it exceeds 60%, the fluidity is deteriorated.

上記のようにして得られるバチルス・ズブチリスの培養液と有機物のキャリアの混合物は水分含量が高く、保存安定性が低いため、乾燥する必要がある。この際100℃を超えるような乾燥方法では菌の生存率が著しく低下するため、100℃以下で行うことが必須である。   The mixture of the culture medium of Bacillus subtilis obtained as described above and an organic carrier has a high water content and low storage stability, and therefore needs to be dried. At this time, the drying method exceeding 100 ° C. significantly reduces the survival rate of the bacteria, so that it is essential to carry out at 100 ° C. or less.

また、減圧下で乾燥させることにより乾燥時間を短縮することができ、菌の生存率を高く維持することができる。乾燥後の水分含量は30質量%以下が好ましく、20質量%以下であるとより好ましい。   Moreover, drying time can be shortened by making it dry under reduced pressure, and the survival rate of a microbe can be maintained high. The moisture content after drying is preferably 30% by mass or less, and more preferably 20% by mass or less.

このようにして得られる本発明の微生物含有組成物は、これ自体で農園芸用資材または土壌改良用資材として使用することも可能であるが、これを添加した有機肥料を製造した上で使用すると土壌に容易に均一に施用できるため、極めて効果的である。   The microorganism-containing composition of the present invention thus obtained can be used as an agricultural or horticultural material or a soil improvement material by itself, but when used after producing an organic fertilizer to which this is added. It is very effective because it can be applied to soil easily and uniformly.

有機肥料が堆肥の場合、堆肥製造工程中、例えば一次堆積槽で本発明の微生物含有組成物を添加する方法、あるいは二次撹拌槽で添加する方法のいずれも可能であるが、二次撹拌槽で添加することが好ましい。本発明の微生物含有組成物の添加量は、堆肥原料当たり質量比で1/10000〜1/100程度が好ましい。このようにして得られる肥料を土壌1m2に対して通常0.1〜10kg施用する。 When the organic fertilizer is compost, either the method of adding the microorganism-containing composition of the present invention in the compost production process, for example, in the primary deposition tank or the method of adding in the secondary stirring tank is possible. It is preferable to add at. The addition amount of the microorganism-containing composition of the present invention is preferably about 1/10000 to 1/100 in terms of mass ratio per compost raw material. The fertilizer thus obtained is usually applied in an amount of 0.1 to 10 kg per 1 m 2 of soil.

以下、実施例により本発明を具体的に説明するが、本発明はこれらの実施例によって何ら制限されるものではない。   EXAMPLES Hereinafter, although an Example demonstrates this invention concretely, this invention is not restrict | limited at all by these Examples.

実施例1
バチルス・ズブチリスSD142を下記組成AのL培地に2%寒天を加えたL平板培地に画線し、35℃で一晩生育させた。そこからL培地3Lを添加した5L容培養槽に植菌し、35℃、600rpmで6時間培養した。さらにL培地150Lを添加した200L容培養槽に植菌し、35℃、400rpmで6時間培養した。次に下記組成Bの培地3000kgを添加した5000L容培養槽に植菌し、30℃、180rpmで70時間培養を行い、バチルス・ズブチリスSD142の培養液を得た。
Example 1
Bacillus subtilis SD142 was streaked on an L plate medium in which 2% agar was added to an L medium having the following composition A, and grown overnight at 35 ° C. From there, it was inoculated into a 5 L culture tank to which 3 L of L medium was added, and cultured at 35 ° C. and 600 rpm for 6 hours. Furthermore, it inoculated in a 200L culture tank which added L culture medium 150L, and culture | cultivated at 35 degreeC and 400 rpm for 6 hours. Next, it was inoculated into a 5000 L culture tank to which 3000 kg of the medium having the following composition B was added, and cultured at 30 ° C. and 180 rpm for 70 hours to obtain a culture solution of Bacillus subtilis SD142.

組成A(L培地) (質量%)
ペプトン 1
酵母エキス 0.5
塩化ナトリウム 0.5
水 残部
Composition A (L medium) (mass%)
Peptone 1
Yeast extract 0.5
Sodium chloride 0.5
Water balance

組成B (質量%)
大豆粉 9
2HPO4 0.5
MgSO4・7H2O 0.05
FeSO4・7H2O 0.0025
MnSO4・5H2O 0.0025
CaCl2 0.1
マルトース 17
水 残部
Composition B (mass%)
Soy flour 9
K 2 HPO 4 0.5
MgSO 4 · 7H 2 O 0.05
FeSO 4 · 7H 2 O 0.0025
MnSO 4 · 5H 2 O 0.0025
CaCl 2 0.1
Maltose 17
Water balance

得られた培養液(水分含量90%)に対し、乾燥ビール粕(水分含量13%)と水を1:4:2の質量比で添加し、スクリューミキサーで撹拌混合し、減圧乾燥機にて60℃で2時間乾燥し、水分含量9%の微生物含有組成物を得た。   To the obtained culture broth (water content 90%), dry beer lees (water content 13%) and water are added at a mass ratio of 1: 4: 2, stirred and mixed with a screw mixer, and then in a vacuum dryer. It was dried at 60 ° C. for 2 hours to obtain a microorganism-containing composition having a water content of 9%.

実施例2
実施例1のうち、乾燥ビール粕の代わりに麦糠(水分含量13%)を用いて同様に行い、水分含量9%の微生物含有組成物を得た。
Example 2
Among Example 1, it carried out similarly using wheat straw (water content 13%) instead of dry beer cake, and obtained the microorganism-containing composition of water content 9%.

比較例1
実施例1のうち、乾燥温度を150℃で行う以外は同様に行い、水分含量4%の微生物含有組成物を得た。
Comparative Example 1
In Example 1, a microorganism-containing composition having a water content of 4% was obtained in the same manner except that the drying temperature was 150 ° C.

比較例2
実施例1のうち、乾燥を行わない以外は同様に行い、水分含量49%の微生物含有組成物を得た。
Comparative Example 2
In Example 1, a microorganism-containing composition having a moisture content of 49% was obtained in the same manner except that drying was not performed.

実施例1、2及び比較例1、2の組成物を室温で保存し、経時的に組成物中の生菌数を測定し、菌体の生存率を算出した。生菌数の測定は、組成物を滅菌水で適宜希釈したものをL平版培地に塗布し、35℃で一晩培養して生育したコロニー数を計測することにより求めた。結果を表1に示す。生存率は実施例1の0日の生菌数を100%としたときの相対値で示した。   The compositions of Examples 1 and 2 and Comparative Examples 1 and 2 were stored at room temperature, the number of viable bacteria in the composition was measured over time, and the survival rate of the cells was calculated. The number of viable bacteria was determined by applying a composition obtained by appropriately diluting the composition with sterilized water to an L lithographic medium, and culturing it overnight at 35 ° C. to count the number of colonies grown. The results are shown in Table 1. The survival rate was expressed as a relative value when the number of viable bacteria on day 0 in Example 1 was taken as 100%.

実施例1、2及び比較例1、2の組成物を各々堆肥製造工程の二次醗酵槽の入口に原料とともに添加して製造した堆肥を得た(実施例3、4、比較例3、4)。組成物の添加量は堆肥に対し質量比で1/100とした。また、組成物を添加せずに製造した堆肥を得た(比較例5)。   Composts produced by adding the compositions of Examples 1 and 2 and Comparative Examples 1 and 2 together with the raw materials to the inlet of the secondary fermenter in the compost production process were obtained (Examples 3 and 4, Comparative Examples 3 and 4). ). The addition amount of the composition was 1/100 in terms of mass ratio with respect to compost. Moreover, the compost produced without adding the composition was obtained (Comparative Example 5).

実施例3、4及び比較例3、4、5の堆肥を室温で保存し、経時的に堆肥中の組成物由来の生菌数を測定し、菌体の生存率を算出した。組成物由来の生菌数の測定は、組成物を滅菌水で適宜希釈した後80℃で30分加熱したものを、2%羊血液を添加したL平版培地に塗布し、35℃で一晩培養して生育したコロニーの周囲にクリアゾーンを形成するコロニー数を計測することにより求めた。結果を表2に示す。生存率は実施例3の0日の生菌数を100%としたときの相対値で示した。   The composts of Examples 3 and 4 and Comparative Examples 3, 4 and 5 were stored at room temperature, the number of viable bacteria derived from the composition in the compost was measured over time, and the survival rate of the bacterial cells was calculated. The number of viable bacteria derived from the composition was measured by appropriately diluting the composition with sterilized water and heating at 80 ° C. for 30 minutes on an L lithographic medium supplemented with 2% sheep blood, and overnight at 35 ° C. It calculated | required by measuring the number of colonies which form a clear zone around the colony grown by culture | cultivation. The results are shown in Table 2. The survival rate was expressed as a relative value when the number of viable bacteria on day 0 in Example 3 was taken as 100%.

実施例3及び比較例5の堆肥を土壌1m2当たり4kg施用して各種農作物の収量を調べた。収量は比較例5(微生物含有組成物を添加しない堆肥)の収量を100としたときの相対値で示した。また、それぞれの栽培における病害発生の有無を示した。結果を表3に示す。 The yield of various agricultural crops was examined by applying 4 kg of the compost of Example 3 and Comparative Example 5 per 1 m 2 of soil. The yield was shown as a relative value when the yield of Comparative Example 5 (compost without addition of the microorganism-containing composition) was taken as 100. Moreover, the presence or absence of the disease occurrence in each cultivation was shown. The results are shown in Table 3.

Claims (14)

土壌病害性微生物に対し拮抗作用を示すバチルス・ズブチリス(Bacillus subtilis)の培養液を有機物のキャリアと混合し、100℃以下の温度で減圧乾燥してなる土壌病害防除能を有する微生物含有組成物。 A microorganism-containing composition having a soil disease control ability obtained by mixing a culture solution of Bacillus subtilis having an antagonistic action against a soil disease-causing microorganism with an organic carrier and drying under reduced pressure at a temperature of 100 ° C. or lower. 粉末状または粒状である請求項1に記載の微生物含有組成物。   The microorganism-containing composition according to claim 1, which is powdery or granular. バチルス・ズブチリスがバチルス・ズブチリス SD142(FERM BP−8427)である請求項1に記載の微生物含有組成物。   The microorganism-containing composition according to claim 1, wherein the Bacillus subtilis is Bacillus subtilis SD142 (FERM BP-8427). 有機物のキャリアが乾燥醗酵粕または麦糠である請求項1に記載の微生物含有組成物。   The microorganism-containing composition according to claim 1, wherein the organic carrier is dried fermented koji or wheat straw. 乾燥後の水分含量が30質量%以下である請求項1乃至4のいずれか1項に記載の微生物含有組成物。   The microorganism-containing composition according to any one of claims 1 to 4, wherein the moisture content after drying is 30% by mass or less. バチルス・ズブチリスの培養液を粉末状または粒状の有機物キャリアと混合し、100℃以下の温度で減圧乾燥することを特徴とする土壌病害防除能を有する微生物含有組成物の製造方法。   A method for producing a microorganism-containing composition having a soil disease control ability, wherein a culture solution of Bacillus subtilis is mixed with a powdered or granular organic carrier and dried under reduced pressure at a temperature of 100 ° C or lower. バチルス・ズブチリスの培養液を有機物キャリアと混合し、100℃以下の温度で減圧乾燥した後、粉末状または粒状とすることを特徴とする土壌病害防除能を有する微生物含有組成物の製造方法。   A method for producing a microorganism-containing composition having a soil disease control ability, wherein a culture solution of Bacillus subtilis is mixed with an organic carrier, dried under reduced pressure at a temperature of 100 ° C. or lower, and then powdered or granular. 請求項1乃至5のいずれか1項に記載の微生物含有組成物を含む農園芸用資材。   An agricultural and horticultural material comprising the microorganism-containing composition according to any one of claims 1 to 5. 請求項1乃至5のいずれか1項に記載の微生物含有組成物を含む土壌改良用資材。   The material for soil improvement containing the microorganisms containing composition of any one of Claims 1 thru | or 5. 請求項1乃至5のいずれか1項に記載の微生物含有組成物および有機肥料を含む肥料。   A fertilizer comprising the microorganism-containing composition according to any one of claims 1 to 5 and an organic fertilizer. 有機肥料が堆肥である請求項10に記載の肥料。   The fertilizer according to claim 10, wherein the organic fertilizer is compost. 有機肥料が液肥である請求項10に記載の肥料。   The fertilizer according to claim 10, wherein the organic fertilizer is liquid fertilizer. 請求項1乃至5のいずれか1項に記載の微生物含有組成物を堆肥製造工程中に添加することを特徴とする堆肥の製造方法。   A method for producing compost, wherein the microorganism-containing composition according to any one of claims 1 to 5 is added during a compost production process. 請求項10乃至12のいずれか1項に記載の肥料を土壌に施用する農作物栽培方法。
A method for cultivating crops, wherein the fertilizer according to any one of claims 10 to 12 is applied to soil.
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WO2007055064A1 (en) * 2005-11-10 2007-05-18 Idemitsu Kosan Co., Ltd. Agent for controlling soil disease
CN101993305A (en) * 2010-10-15 2011-03-30 秦皇岛领先科技发展有限公司 Composite microbial agent and production method thereof
JP2011084449A (en) * 2009-10-19 2011-04-28 Kenichi Sato Agricultural material, microbial material, organic humus fertilizer, water cleaning material, soil conditioner, feed additive, waste treatment agent, roof top greening material, and method for manufacturing the agricultural material
CN102796668A (en) * 2012-08-14 2012-11-28 叶城县金秋实业生物肥有限公司 Preparation method of fermentation microbial inoculum for reinforcing and promoting organic fertilizer compost
CN112851421A (en) * 2019-11-26 2021-05-28 高绍林 Organic fertilizer
CN113462395A (en) * 2021-06-28 2021-10-01 锦州市科学技术研究院 Method for improving soil by combined action of mixed microbial inoculum and chemical fertilizer

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JPH0477429A (en) * 1990-07-18 1992-03-11 Shuzo Nakazono Dehydration of starfish with hot oil
JP2002308714A (en) * 2001-01-23 2002-10-23 National Institute Of Advanced Industrial & Technology Method for producing biological pesticide and biological pesticide
JP2004002390A (en) * 2002-04-18 2004-01-08 National Institute Of Advanced Industrial & Technology Microbial material and production method of material

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007055064A1 (en) * 2005-11-10 2007-05-18 Idemitsu Kosan Co., Ltd. Agent for controlling soil disease
JP2011084449A (en) * 2009-10-19 2011-04-28 Kenichi Sato Agricultural material, microbial material, organic humus fertilizer, water cleaning material, soil conditioner, feed additive, waste treatment agent, roof top greening material, and method for manufacturing the agricultural material
CN101993305A (en) * 2010-10-15 2011-03-30 秦皇岛领先科技发展有限公司 Composite microbial agent and production method thereof
CN102796668A (en) * 2012-08-14 2012-11-28 叶城县金秋实业生物肥有限公司 Preparation method of fermentation microbial inoculum for reinforcing and promoting organic fertilizer compost
CN112851421A (en) * 2019-11-26 2021-05-28 高绍林 Organic fertilizer
CN113462395A (en) * 2021-06-28 2021-10-01 锦州市科学技术研究院 Method for improving soil by combined action of mixed microbial inoculum and chemical fertilizer

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