CN1054258C - Process for mechanized prodn. of granular strains - Google Patents
Process for mechanized prodn. of granular strains Download PDFInfo
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- CN1054258C CN1054258C CN95118473A CN95118473A CN1054258C CN 1054258 C CN1054258 C CN 1054258C CN 95118473 A CN95118473 A CN 95118473A CN 95118473 A CN95118473 A CN 95118473A CN 1054258 C CN1054258 C CN 1054258C
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Abstract
The present invention relates to a process for the mechanical production of granular bacteria. The present invention comprises the process steps of mother-seed and original-seed preparation, liquid static-placement cultivation, shaking cultivation, three-stage fermentation tank system cultivation, liquid-seed storage tank entering, and diaphragm conveying, material filling, inoculaton, cover pressing, etc. by automatic completion of a machine for preparing granular bacteria; then, the diaphragms enter a greenhouse to become the formed granular bacteria through cultivation. The present invention has the characteristics of high mechanization degree, shortened production period, fewer opportunities of pollution of mixed bacterium, improved rate of finished products, production cost reduction, etc. The present invention is suitable for the production of domestic fungus or other microorganism granular bacteria.
Description
The invention belongs to particle kind production technology and the equipment of edible mushroom and other microorganism fungus kind.
Domestic edible fungus species production is produced based on bottled and packed wood chip bacterium form, the novel particle kind only has part Study at home and abroad, granular bacteria strain and the introduction spring nineteen ninety-five taken back according to the Luo Xinchang of Central China agricultural university professor from Japan, its technology for bottled or packed wood chip bacterial classification dig broken after, insert in the hole of plastic mold pieces, after machinery presses cover plate, again through cultivating the mycelium germination growth shaping.Though the method is produced particle kind with cover, use easily, but its production cycle is longer, (does not contain female the kind because the production of bottled wood chip bacterial classification needs 35-40 days, the original seed production time) dig kind sprouting moulding on matrix and only need 7-10 days time, time expand, increased operation.Leading portion wood chip bacterial classification is produced by manual in this technology, and the time is long, though the back segment moulding can be used machinery, front and back are difficult for combination, are not easy to mechanization and produce in a large number.The domestic Guangxi Academy Of Sciences institute of microbiology technical study project that " moulding bacterial classification " made and batch production is produced that in December, 1991, research was finished, number of patent application 88101366.8, its process route chart are Fig. 5.Its production technology is: make the female kind of PDA test tube routinely, make bottled wood chip original seed after 12-15 days maturations, expanding again under 25 ℃ ± 1 ℃ condition, bottled wood chip original seed under 25 ℃ ± 1 ℃ condition through grew in 40-45 days ripe after, digging broken back inserts in the bottled liquid nutrient medium of triangle, triangular flask put and carry out shaken cultivation on the shaking table, cultivating through 4 days under 25 ℃+1 ℃ condition is the level liquid kind, switching is gone in the compact simplified fermentation tank (aluminium pot or serum ware that 10-15 rises) again, be the secondary liquid strain after cultivating in 4 days under 25 ℃ ± 1 ℃, it is promptly used as the production of shaped granule kind.The solid culture medium for preparing is 1: 0.8 a ratio in solid culture medium and secondary liquid strain after sterilization, and mix is evenly afterwards standby.The PVC matrix of plastics sucking moulding is placed on the special-purpose supporting plate, and the composts or fertilisers of cultivating that will mix liquid strain with spoon or shovel digs out and is poured on the matrix again, and hand makes composts or fertilisers of cultivating fall into the depression of matrix by veneer.Above-mentioned onesize PVB bubble of PVC matrix loam cake that fills up material is moulded sheet material, thick is the 3-4 millimeter, push in the capping machine that designs and produces voluntarily together with the matrix supporting plate, it is die-cut and be pressed in the depression of matrix that PVB bubble is moulded sheet material, the capping composts or fertilisers of cultivating, matrix is moved into culturing room under 25 ℃ of conditions, cultivated, be shaped granule kind finished product through 10 days.
In the technical process of last figure, except that PVC, the processing of PVB plastic sheet are leaned on the machinery, only covering the lid lid machine batch (-type) of developing in the project for this reason finishes, but solid culture medium and liquid strain mix and these two operations of filler need manually be finished, the automatic and mechanical degree is low, and mixing and filler manual operations time are long, very easily be bacterial contamination, also the mixed proportion because of solid culture medium and liquid strain is 1: 0.8, so the liquid strain consumption is big, the fermenter equipment investment is bigger.The main cause that the research of this technology fails to apply at present is that living contaminants is more serious, and yield rate is difficult to improve.
The purpose of this invention is to provide operations such as a kind of conveying, filler, access liquid strain, gland with plastic mold pieces by production of hybrid seeds machine finish automatically continuously, the mechanization degree height, the liquid strain consumption is few, granular strains mechanical productive technology and special equipment that yield rate is high.
The object of the present invention is achieved like this: a kind of granular strains mechanical productive technology, it is characterized in that production technology carries out as follows, and the bacterial classification volume is by producing 500 liters of liquid strains every day:
A, the female kind are produced: 25 ℃ of temperature, time 10-12 days;
B, wood chip original seed are produced: 25 ℃ of temperature, time 35-40 days;
C, liquid leave standstill cultivation: become shape in small, broken bits to insert in the triangular flask liquid control of wood chip original seed, each triangular flask is 100 milliliters, and each adorns 20 milliliters of liquid nutrient mediums, and 25 ± 1 ℃ of medium temperature leave standstill and cultivated 96 hours, shake every day once;
D, shaken cultivation: after treating that mycelium germination is produced, change three 100 milliliters of triangular flasks over to, respectively adorn and carry out shake-flask seed cultivation, 25 ℃ of temperature, 180 rev/mins of shaking speed, incubation time 48 hours in the 20 milliliters of liquid medium;
E, three grade fermemtation jar are cultivated: the kind jar of the aforesaid liquid kind being put into the three grade fermemtation can system is cultivated, and the one-level jar is 7 liters of seeding tank volumes, 25 ℃ of temperature, incubation time 64 hours, secondary jar are the one grade fermemtation jar, 70 liters of volumes, 25 ℃ of medium temperature, 64 hours time, three grades of jars are the second order fermentation jar, 700 liters of volumes, 25 ℃ of medium temperature, 72 hours time, wherein coefficient 70 ± 1%, inoculation is 10%, gets 500 liters of liquid spawns; Be pressed in the basin standby;
F, solid culture medium preparation are after the sterilization cooling is standby;
G, plastic sheet are processed into matrix through plastic uptake, and bubble is moulded and cut into bubble through slitter and mould cover plate;
H, the liquid strain basin 0.03-0.05MPa that keep-ups pressure is connected with production of hybrid seeds machine liquid strain feed pipe joint with sebific duct, and the composts or fertilisers of cultivating of will sterilizing adds material bin; The volume ratio of solid culture medium and liquid strain inoculation is 1: 0.16;
I, plastic mold pieces and bubble moulded each inlet feeding of cover plate after, can start by frame, the running part that is provided with in matrix supporting plate that the frame bottom is provided with and the frame, matrix conveying plant, sterilization composts or fertilisers of cultivating are inserted the filler device of matrix, liquid strain classification inoculation apparatus and Jiang Pao mould the granular strains production of hybrid seeds machine that lid is pressed into the pressing device composition in matrix hole, finish conveying, filler, inoculation and the gland technology of matrix;
Enter after j, matrix form in the greenhouse and cultivate, 25 ℃ of temperature can be produced ripe granular strains with cover through 7-10 days.
Be exclusively used in the granular strains production of hybrid seeds machine of the matrix conveying described in the granular strains mechanical productive technology, filler, inoculation, cap pressing process.It comprises frame 27, the running part that is provided with in matrix supporting plate 25 that the frame bottom is provided with and the frame, and conveying plant, filler device, classification inoculation apparatus, pressing device are formed; Running part is made up of each gear that collection is loaded in the gear-box, main shaft is by motor-driven: conveying plant send matrix axle 2 by what drive that matrix linearly moves, 4,6 form, on the axle pulldown claw 26 is set, filler device is by hopper 24, the interior stirring pawl 23 that is provided with of hopper, hopper bottom are provided with material-dropping hole 28 to be formed with the frame 5 that gathers materials on the frame 27, blanking axle 3 blanking axles is set by transmission device and 23 interlocks of stirring pawl in the frame that gathers materials; The classification inoculation apparatus that is positioned at behind the filler device is made up of overcoat 7 and mandrel 21, the feed pipe joint 20 that is connected by pipeline with the liquid spawn basin is set on the overcoat 7, the corresponding discharge nozzle in hole on setting of overcoat below and the matrix 1, the through hole and the material chamber 22 of aliging with discharge nozzle are set on the mandrel 21, in each through hole on the mandrel slip valve rod 9 are set; Pressing device is by sending cover plate axle 14, cover plate punch-head assembly and drive the gland ratchet wheel 19 that punch-head assembly moves up and down, be positioned at the waste material auger 10 that removes that reaches the punch-head assembly side on the frame to form.Conveying plant is made up of at least three identical matrix axles that send, and on the axle a plurality of pulldown claws 26 is set.Pulldown claw 26 spacings equate with hole spacing on the matrix 1.Punch-head assembly with the drift 15 that projection axially is connected, is enclosed within the pressing device fixed head 17 on the projection by projection 18, is provided with on the pressing device fixed head to cut knife plate 12 compositions that bubble is moulded the blade drift below of cover plate.Gather materials in the frame and the frame that gathers materials respectively is provided with one along direction of motion both sides and send the matrix axle.
The advantage of this technology relatively is with the shaped granule kind of Japan: (1) can shorten production cycle 20-25 days; (2) can thoroughly reform the present manually-operated mode of production of leaning on, realize the large-scale production of mechanization.
Compare with the process route of Guangxi Academy Biological Research Institute, its advantage is: (1) mechanization degree height, can realize automatic continuous operation; (2) the plant equipment closure of being developed is good, running operation continuously automatically, and the time is short, and the living contaminants chance is few, and yield rate guarantees easily.(3) liquid strain has avoided the liquid strain mycelium pellet in the damage that mixes in stirring by production of hybrid seeds machine automatic vaccination in the technology, so sprout soon, not only incubation time can shorten and easier raising yield rate; (4) this process solids composts or fertilisers of cultivating is 1: 0.16 with the ratio of liquid strain inoculum concentration, and mixed stirring relatively can significantly reduce the liquid strain consumption reached 80% with its 1: 0.8, had reduced production cost, under the identical output situation, and the investment that can save fermenter equipment.
Describe the present invention in detail below in conjunction with drawings and Examples.
Fig. 1 is a process chart of the present invention,
Fig. 2 is the structural representation of granular strains production of hybrid seeds machine,
Fig. 3 is a matrix supporting plate vertical view,
Fig. 4 is the classification inoculation apparatus schematic diagram,
Fig. 5 is the process chart of prior art.
The big I of day production capacity of this technology designs as the case may be, below by producing 500 liters every day, can be made into 25000 of type particle kinds, promptly the scale of every 126 particle kinds specifies its technological process.
Be ready to female the kind as scheduled, original seed, the wood chip original seed is scraped out shape in small, broken bits insert triangular flask liquid (three 100ml bottles, each adorns liquid nutrient medium 20ml) temperature leaves standstill for about 25 ℃ and cultivated 96 hours in the medium, shake every day once, treating promptly to change over to after the mycelium germination production three 1000ml triangular flasks respectively adorns and carries out shake-flask seed in the 200ml liquid nutrient medium and cultivate, 25 ℃ of temperature, 180 rev/mins, the seed tank culture of cultivating in the three grade fermemtation can system that is about to 700v through 48 hours, seeding tank is 7 liters, the one-level jar is 70 liters, the secondary jar is 700 liters, coefficient is about 70%, and inoculation is 10%, and the three grade fermemtation jar can obtain liquid spawn 500l after cultivating and finishing.Be pressed in the basin standby.Solid culture medium is prepared after the sterilization cooling is standby according to arrangement of time; Plastic sheet is processed into matrix through Packing Machine; Bubble is moulded and is cut into bubble through slitter and mould cover plate.
When above operation finishes, the liquid strain basin 0.03-0.05Mpa that keep-ups pressure is connected with production of hybrid seeds machine liquid feed pipe joint with sebific duct, the composts or fertilisers of cultivating of will sterilizing adds material bin, constantly send from outlet after conveying, filler, inoculation and the gland of plastic mold pieces and cover plate finished continuously by production of hybrid seeds machine, after cutting into certain specification, enter hot-house culture, 25 ℃ of temperature can growth and maturity be the particle kind with cover of moulding through 7-10 days.
The Preliminary design of " the granular strains production of hybrid seeds machine " that uses in this technology is produced 6 (every last 126 particle kind P * 14) as per minute, 8600 fermenter systems of every machine daily output are produced 500 liters of liquid strains every day, can produce 25000 of granular strains (3,150,000) by 1: 0.16 inoculum concentration of this technological design, so need the production of three production of hybrid seeds machines on stream.
The automated production of granular strains production of hybrid seeds machine is achieved in that in the process that the matrix conveying axis is constantly sent into matrix, finish filler, inoculation and gland operation respectively successively by filler device, classification inoculation apparatus, pressing device, send and cut into slices through machine exit and after cultivating about 7 days, be every with some granular strains.All devices are loaded on each gear drive in the gear-box by collection, and main gear shaft is by the driven by motor of 0.7-1.5KW, and each gear is realized harmonious and automatic continuous running operation under the cooperation of certain rotating ratio.Conveying plant has three identical matrix axles 2,4,6 that send, and the pulldown claw of some is installed on every axle vertically, and pawl spacing (axially) equates with hole spacing on the matrix, with the rotation pulldown claw of axle matrix is fed forward by rows; Filler device by can closed hopper 24, stir pawl 23.The blanking axle 3 and frame 5 compositions that gather materials stir pawl 23 continuous rotations in case moistening art is considered the bin sky to be worth doing and driven blanking axle 3 again and again downward blankings, when the second and the 3rd send the matrix axle to carry matrix, the wood chip material are pressed in the hole of plastic mold pieces; Classification inoculation apparatus is made up of a fixing overcoat 7 and a mandrel 21, there is a feed pipe joint 20 to link to each other with the liquid spawn basin on the overcoat 7 with sebific duct, there is the corresponding discharge nozzle 8 in hole on a row and the matrix overcoat below, the through hole that on mandrel 21, has a row to align with discharge nozzle, there is a slidable valve rod 9 in boring ratio discharge nozzle aperture on the mandrel in little each hole on mandrel, and valve rod is at liquid strain basin 0.03-0.05MPa/cm
2Pressure and the effect of own wt under finish the synchronization action of charging and discharging; Pressing device is made up of the row's drift 15 that send cover plate axle 14, gland ratchet wheel 19, removes waste material auger 10 and assembling, after bubble is moulded cover plate 13 and is sent into, under the pressure of 19 pairs of projections 18 of gland ratchet wheel, promote row's drift and mould the cover plate punching press to bubble, be pressed into after knife plate is die-cut into little garden sheet in the matrix hole of filler and inoculation, bubble is moulded the clout of cover plate after die-cut and is entered except that waste material auger 10 discharge machines by the blade that is installed together with drift 15 shape that cuts into dices.
The speed of production of this production of hybrid seeds machine can realize at decelerator between main shaft and motor and belt pulley adjusted rotating speed.But at rotating speed one regularly, also can change the design length that send matrix axle and classification inoculation apparatus, pressing device, and the width of corresponding change matrix is how much realizing of every round cave quantity.This production of hybrid seeds machine also can carry out the production of other microbial solid bacterial classification simultaneously based on edible fungus species production.
To be the PVC plastic sheet be processed into the matrix 1 in 13 millimeters of the diameters that gather, dark 13 millimeters circular cone cylindricality hole through plastic uptake to plastic mold pieces 1, and after inlet fed certainly, first send matrix axle 2 to be about to matrix 1 constantly was fed forward, and begins filler after arriving frame 5 belows that gather materials.Filler is under the stirring pawl 23 continuous condition of stirring in hopper 24, stirs by stirring pawl 23 that blade on the blanking axle 3 falls moistening wood chip material again and again.Second send matrix axle 4 in sending the process of matrix, the more important thing is on the axle send the sheet pawl play material grasping and and the matrix hole in the effect of compacting, continue to insert the vacant part in matrix hole thereafter again by the part clout in the frame 5 that gathers materials.Matrix 1 moves on, the 3rd send matrix axle 6 in conveying for the second time with the material compacting in the hole to reach the full purpose of filler in the matrix hole, when matrix 1 advances to the classification inoculation apparatus below, be connected 0.03-0.05MPa/cm in jar with the liquid strain basin through sebific duct by liquid strain feed pipe joint 20
2The liquid under pressure kind enter in the material chamber 22 of classification inoculation apparatus mandrel 21, when inoculation mandrel 21 rotations 180 degree communicate with discharge nozzle 8, under the pressure in the liquid strain basin valve rod 9 is promoted downwards, so discharging inoculation and top charging are finished synchronously, the aperture of discharge nozzle 8 is φ 3.5mm, less than the diameter of valve rod φ 5.9mm valve rod is deviate from.Bubble mould cover plate 13 enter under the propelling movement of sending the two thorn of cover plate over glaze 14 knife plate 12 above, promote projection 18 during 19 rotations of gland ratchet wheel and drift 15 is moulded cover plate 13 punching presses to bubble, the hole that on the knife plate 12 is the pitch-row of a row φ 13.5mm and matrix is apart from identical.Under dashing of drift 15 taken shear action with knife plate 12 by force, bubble is moulded cover plate be washed into little garden sheet and be pressed on the matrix in the hole of filler and inoculation.Behind the gland bubble mould on the cover plate 13 enter after waste material is cut off by blade 16 between remaining hole except that waste material auger 10 by the external ash can that falls into of screw-conveyer-shaft 11 continuous kickoff mechanisms.Finish behind the above all processes matrix and constantly send from outlet, be cut into a certain size specification as required after, put into the cultivation of greenhouse about 7 days and be a material type bacterial classification with cover.
Claims (3)
1, a kind of granular strains mechanical productive technology is characterized in that production technology carries out as follows, and the bacterial classification volume is by producing 500 liters of liquid strains every day:
A, the female kind are produced: 25 ℃ of temperature, time 10-12 days;
B, wood chip original seed are produced: 25 ℃ of temperature, time 35-40 days;
C, liquid leave standstill cultivation: the wood chip original seed is scraped out shape in small, broken bits insert in the triangular flask liquid, each triangular flask is 100 milliliters, and each adorns 20 milliliters of liquid nutrient mediums, and 25 ± 1 ℃ of medium temperature leave standstill and cultivated 96 hours, shake every day once;
D, shaken cultivation: after treating that mycelium germination is produced, change three 100 milliliters of triangular flasks over to, respectively adorn and carry out shake-flask seed cultivation, 25 ℃ of temperature, 180 rev/mins of shaking speed, incubation time 48 hours in the 20 milliliters of liquid medium;
E, three grade fermemtation jar are cultivated: the kind jar of the aforesaid liquid kind being put into the three grade fermemtation can system is cultivated, and the one-level jar is 7 liters of seeding tank volumes, 25 ℃ of temperature, incubation time 64 hours, secondary jar are the one grade fermemtation jar, 70 liters of volumes, 25 ℃ of medium temperature, 64 hours time, three grades of jars are the second order fermentation jar, 700 liters of volumes, 25 ℃ of medium temperature, 72 hours time, wherein coefficient 70 ± 1%, inoculation is 10%, gets 500 liters of liquid spawns; Be pressed in the basin standby;
F, solid culture medium preparation are after the sterilization cooling is standby;
G, plastic sheet are processed into plastic mold pieces through plastic uptake, and bubble is moulded and cut into bubble through slitter and mould cover plate;
H, the liquid strain basin 0.03-0.05MPa that keep-ups pressure is connected with production of hybrid seeds machine liquid strain feed pipe joint with sebific duct, and the composts or fertilisers of cultivating of will sterilizing adds material bin; The volume ratio of solid culture medium and liquid strain inoculation is 1: 0.16;
I, plastic mold pieces and bubble moulded each inlet feeding of cover plate after, can start by frame, the running part that is provided with in matrix supporting plate that the frame bottom is provided with and the frame, the matrix conveying plant, the sterilization composts or fertilisers of cultivating is inserted the filler device of matrix, liquid strain classification inoculation apparatus and Jiang Pao mould the granular strains production of hybrid seeds machine that lid is pressed into the pressing device composition in matrix hole, finish conveying, filler, inoculation and the gland technology of matrix:
Enter after j, matrix form in the greenhouse and cultivate, 25 ℃ of temperature can be produced ripe granular strains with cover through 7-10 days.
2, a kind of granular strains production of hybrid seeds machine that is exclusively used in the matrix conveying described in the granular strains mechanical productive technology, filler, inoculation, cap pressing process, it is characterized in that: it comprises frame (27), the running part that is provided with in matrix supporting plate that the frame bottom is provided with and the frame, conveying plant, filler device, classification inoculation apparatus, pressing device are formed, running part is made up of each gear that collection is loaded in the gear-box, and main shaft is by motor-driven; Conveying plant send matrix axle (2 by what drive that matrix (1) linearly moves, 4,6) form, on the axle pulldown claw (26) is set, filler device is by hopper (24), the interior stirring pawl (23) that is provided with of hopper, hopper bottom are provided with material-dropping hole (28) to be formed with the frame that gathers materials (5) on the frame (27), blanking axle (3) blanking axle is set by transmission device and stirring pawl (23) interlock in gather materials (5); The classification inoculation apparatus that is positioned at behind the filler device is made up of overcoat (7) and mandrel (21), overcoat (7) is gone up the feed pipe joint (20) that is connected by pipeline with the liquid spawn basin is set, the corresponding discharge nozzle in hole on setting of overcoat below and the matrix, mandrel (21) is gone up the through hole and the material chamber (22) of aliging with discharge nozzle is set, and slip valve rod (9) is set in each through hole on the mandrel; Pressing device is by sending cover plate axle (14), cover plate punch-head assembly and drive the gland ratchet wheel (19) that punch-head assembly moves up and down, be positioned at the waste material auger (10) that removes that reaches the punch-head assembly side on the frame to form; Conveying plant is made up of at least three identical matrix axles that send, and on the axle a plurality of pulldown claws (26) is set; Pulldown claw (26) spacing equates with hole spacing on the matrix; Punch-head assembly with the drift (15) that projection axially is connected, is enclosed within the pressing device fixed head (17) on the projection by projection (18), is provided with on the pressing device fixed head to cut blade and the composition of the knife plate (12) below the drift that bubble is moulded cover plate.
3, granular strains production of hybrid seeds machine according to claim 2 is characterized in that gathering materials in the frame and the frame that gathers materials respectively is provided with one along direction of motion both sides and send the matrix axle.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN95118473A CN1054258C (en) | 1995-10-31 | 1995-10-31 | Process for mechanized prodn. of granular strains |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN95118473A CN1054258C (en) | 1995-10-31 | 1995-10-31 | Process for mechanized prodn. of granular strains |
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| CN1134780A CN1134780A (en) | 1996-11-06 |
| CN1054258C true CN1054258C (en) | 2000-07-12 |
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| CN95118473A Expired - Fee Related CN1054258C (en) | 1995-10-31 | 1995-10-31 | Process for mechanized prodn. of granular strains |
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| CN106416749B (en) * | 2016-08-31 | 2020-02-07 | 四川宝生药业发展有限公司 | Method for reducing operation pollution in liquid strain culture process |
| CN111357570A (en) * | 2020-04-25 | 2020-07-03 | 广西壮族自治区亚热带作物研究所（广西亚热带农产品加工研究所） | Production method of three-level strain for black fungus cultivated in plastic bag field |
| CN111480518B (en) * | 2020-05-28 | 2021-09-24 | 杨凌知君菌业科技工程有限责任公司 | Edible mushroom production method |
| CN113088431A (en) * | 2021-04-28 | 2021-07-09 | 贵州同辉食用菌发展有限公司 | High-activity and high-stability shiitake mushroom liquid strain fermentation system and fermentation method |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN86100509A (en) * | 1986-02-04 | 1986-07-30 | 上海师范大学 | A kind of technology with deep culturing mycelium for mushroom breeding |
| CN88101366A (en) * | 1988-03-10 | 1988-12-28 | 广西科学院生物研究室 | Novel strain production process |
| CN1048481A (en) * | 1989-07-06 | 1991-01-16 | 杨书堂 | The preparation method of a kind of edible mushroom template solid spawn |
| CN1060572A (en) * | 1990-10-11 | 1992-04-29 | 即墨市科委食用菌研究所 | The making method of mycoderm edible fungus species |
| CN2234444Y (en) * | 1995-10-31 | 1996-09-04 | 远安县森源食用菌公司 | Granular pure culture preparing machine |
-
1995
- 1995-10-31 CN CN95118473A patent/CN1054258C/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN86100509A (en) * | 1986-02-04 | 1986-07-30 | 上海师范大学 | A kind of technology with deep culturing mycelium for mushroom breeding |
| CN88101366A (en) * | 1988-03-10 | 1988-12-28 | 广西科学院生物研究室 | Novel strain production process |
| CN1048481A (en) * | 1989-07-06 | 1991-01-16 | 杨书堂 | The preparation method of a kind of edible mushroom template solid spawn |
| CN1060572A (en) * | 1990-10-11 | 1992-04-29 | 即墨市科委食用菌研究所 | The making method of mycoderm edible fungus species |
| CN2234444Y (en) * | 1995-10-31 | 1996-09-04 | 远安县森源食用菌公司 | Granular pure culture preparing machine |
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