CN88101366A - Novel strain production process - Google Patents
Novel strain production process Download PDFInfo
- Publication number
- CN88101366A CN88101366A CN88101366.8A CN88101366A CN88101366A CN 88101366 A CN88101366 A CN 88101366A CN 88101366 A CN88101366 A CN 88101366A CN 88101366 A CN88101366 A CN 88101366A
- Authority
- CN
- China
- Prior art keywords
- inoculated
- wood
- production process
- culture
- culture medium
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000004519 manufacturing process Methods 0.000 title abstract description 13
- 239000002023 wood Substances 0.000 claims abstract description 11
- 238000000034 method Methods 0.000 claims abstract description 7
- 239000007787 solid Substances 0.000 claims abstract description 7
- 238000000855 fermentation Methods 0.000 claims abstract description 5
- 230000004151 fermentation Effects 0.000 claims abstract description 5
- 238000012258 culturing Methods 0.000 claims abstract 2
- 239000008188 pellet Substances 0.000 claims description 5
- 239000007788 liquid Substances 0.000 claims description 4
- 238000000465 moulding Methods 0.000 claims description 4
- 239000012530 fluid Substances 0.000 claims description 3
- 235000015097 nutrients Nutrition 0.000 claims description 3
- 241000233866 Fungi Species 0.000 abstract description 5
- 239000000428 dust Substances 0.000 abstract description 3
- 239000002245 particle Substances 0.000 abstract description 3
- 238000011081 inoculation Methods 0.000 abstract description 2
- 239000001963 growth medium Substances 0.000 abstract 2
- 238000009630 liquid culture Methods 0.000 abstract 1
- 235000015073 liquid stocks Nutrition 0.000 abstract 1
- 238000005516 engineering process Methods 0.000 description 6
- 235000001674 Agaricus brunnescens Nutrition 0.000 description 3
- 230000006872 improvement Effects 0.000 description 3
- 230000008901 benefit Effects 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 230000035764 nutrition Effects 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 238000009776 industrial production Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
Abstract
A process for preparing edible fungus spawn features that the wood-rotting fungus spawn used for culturing log is made into particles with capped wood dust, so greatly simplifying the inoculation procedure. The method is mainly characterized in that mother seeds are inoculated into a liquid culture medium for submerged fermentation and expanded culture, and then the obtained liquid stock seeds are inoculated onto a solid culture medium for mold filling and culture forming.
Description
The invention belongs to the edible fungus species manufacture craft.
Along with population increases and the living standards of the people raising, adjusting and improving the food nutrition structure has become a current grand strategy problem that faces.Edible mushroom is because its high-quality nutrition formation, make it in the new food resource of exploitation, occupy more and more important position, in order to develop Edible Fungi, a series of improvement have been carried out in the strain improvement of edible mushroom and making, cultivation technique, product processing etc. both at home and abroad.Aspect the wood destroying fungi bacterial classification making of segment wood cultivated use, develop into wood pellet kind with cover from original wood chip kind, clavate kind.Particle kind with cover has cultivation inoculation operation simple (only need on Duan Mu perforating and put kind of a two procedures), efficient and survival rate, output, economic benefit geometric ratio are used all high remarkable advantages of wood chip kind, clavate kind, but cost of manufacture also wants high than wood chip kind and clavate kind.(richness is guarantor forever according to " improvement of mushroom strain and progress ", day, 1986) data is described, its technology of making capped saw dust particulate original seed be with mother plant be inoculated into solid medium and carry out cellar culture (generally needing 35～40 days) after, obtaining solid original seed (secondary kind) inoculated carry out cellar culture (usually need 65 days) on the solid medium, just obtain to produce kind (three grades of kinds).In order to make wood pellet raw material with cover, must will produce kind of the broken dress mould of stranding and recover moulding (taking approximately about 7 days).Adopt this production of hybrid seeds technology, need the long period, and when production kind of stranding is broken, mycelia is damaged.In order to shorten the production of hybrid seeds time, the production of hybrid seeds can be carried out with large-scale industrial production, thereby cost is lowered, we reform this production of hybrid seeds technology.
The present invention realizes as follows: with mother plant be inoculated into carry out submerged fermentation in the fluid nutrient medium and enlarge cultivate after, the liquid strain that obtains is inoculated on the solid medium dress mould and cultivates moulding, obtain capped saw dust particulate original seed.Use this technology to make wood pellet original seed with cover, the time of making only needs 24～31 days, compare with above-mentioned day present technique, both simplified production of hybrid seeds technology, time and manpower and materials have been saved again, and owing to adopted advanced fermentation technique, make the production of hybrid seeds can realize mechanize, automatic production, these are all favourable to reducing breeding cost.In addition, also because the particle kind does not sustain damage in manufacturing process, make its bacterium effect better.
Below be an embodiment of this technology:
Mother planted to receive carry out submerged fermentation behind the fluid nutrient medium and cultivate, after 7 days the gained liquid strain is enlarged cultivation again, through 7-14 days (time length is determined on a case-by-case basis) obtaining liquid strain is inoculated in again and fills mould on the solid medium, cultivate moulding, obtain wood pellet kind with cover after 10 days.
Claims (1)
- A kind of new mushroom-seed culturing process is made wood pellet with cover with original seed, it is characterized in that with mother plant be inoculated into carry out submerged fermentation in the fluid nutrient medium and enlarge cultivate after, the liquid original seed that obtains is inoculated in the enterprising luggage mould of solid medium and cultivates moulding.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 88101366 CN1025790C (en) | 1988-03-10 | 1988-03-10 | New Mushroom-seed culturing process |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 88101366 CN1025790C (en) | 1988-03-10 | 1988-03-10 | New Mushroom-seed culturing process |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN88101366A true CN88101366A (en) | 1988-12-28 |
| CN1025790C CN1025790C (en) | 1994-08-31 |
Family
ID=4831727
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 88101366 Expired - Fee Related CN1025790C (en) | 1988-03-10 | 1988-03-10 | New Mushroom-seed culturing process |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1025790C (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1054258C (en) * | 1995-10-31 | 2000-07-12 | 远安县森源食用菌公司 | Process for mechanized prodn. of granular strains |
| CN102972206A (en) * | 2012-12-10 | 2013-03-20 | 无限极（中国）有限公司 | Artificial cultivation method and culture medium of lucid ganoderma |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101265454B (en) * | 2008-04-03 | 2010-06-02 | 武汉市武湖食用菌高新技术开发有限公司 | Cultivation of edible mushroom composition by using asparagus bar and cultivating method thereof |
-
1988
- 1988-03-10 CN CN 88101366 patent/CN1025790C/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1054258C (en) * | 1995-10-31 | 2000-07-12 | 远安县森源食用菌公司 | Process for mechanized prodn. of granular strains |
| CN102972206A (en) * | 2012-12-10 | 2013-03-20 | 无限极（中国）有限公司 | Artificial cultivation method and culture medium of lucid ganoderma |
| CN102972206B (en) * | 2012-12-10 | 2015-01-28 | 无限极（中国）有限公司 | Artificial cultivation method and culture medium of lucid ganoderma |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1025790C (en) | 1994-08-31 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102440147A (en) | Production technology of edible mushroom liquid spawn by adopting reducing process | |
| CN102783356A (en) | Method for converting edible mushroom solid strain into liquid strain | |
| CN104206169A (en) | Method for preparing nutrient cereal by cordyceps militaris culture medium | |
| CN111248026B (en) | Quercus matsutake culture medium and application thereof | |
| CN104855141A (en) | Edible mushroom liquid strain preparation method based on ultrasonic crushing | |
| CN103923908A (en) | Preparation of edible fungi immobilized bacteria and preservation method thereof | |
| CN105379556A (en) | Industrial pleurotus erygii cultivation method adopting temperature-controlled fruiting management technique | |
| CN102726211A (en) | Method for preparing artificially domesticated original strains of wild red-soil termitomyces albuminosus | |
| CN110754299B (en) | Method for bottle cultivation of poria cocos sclerotia | |
| CN113475310A (en) | Method for controlling formation of primordium number and primordium differentiation and development of industrial bag-cultured pleurotus nebrodensis | |
| CN1025790C (en) | New Mushroom-seed culturing process | |
| CN109971653B (en) | Rapid rejuvenation method for needle mushroom degenerated strains | |
| CN106718016A (en) | A kind of earthing matrix for improving agaricus bisporus fruit-body formation amount and preparation method thereof | |
| CN102021212A (en) | Preparation method of ganoderma polysaccharide | |
| CN1944625A (en) | Coterpillar fungus hypha cultivating method of rice solid fermenting for caterpillar fungus drink | |
| CN104498542A (en) | Method for preparing L-lactic acid employing continuous method in fermentation manner | |
| CN114158424A (en) | Needle mushroom bottling and punching method | |
| CN111699922A (en) | Needle mushroom strain preservation culture medium, needle mushroom culture material and application thereof | |
| CN1965634A (en) | Method for producing capped saw dust particulate original seed | |
| CN219834975U (en) | Honeycomb culture body for mushroom propagation | |
| CN105410034B (en) | A kind of compound accelerant of cordyceps sinensis cultivation and preparation method thereof | |
| CN114395488B (en) | Culture medium for promoting growth of single-core mycelia of lentinus edodes and preparation method thereof | |
| CN113748925B (en) | Mushroom culture medium and preparation method and culture method thereof | |
| CN1504073A (en) | Mushroom cultivation process | |
| CN115380764B (en) | Xylopsis fragrans and domestication cultivation method of Xylopsis fragrans |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| C14 | Grant of patent or utility model | ||
| C19 | Lapse of patent right due to non-payment of the annual fee |