CN105399530A - Preparation and application of composite microbial fertilizer - Google Patents
Preparation and application of composite microbial fertilizer Download PDFInfo
- Publication number
- CN105399530A CN105399530A CN201510948181.5A CN201510948181A CN105399530A CN 105399530 A CN105399530 A CN 105399530A CN 201510948181 A CN201510948181 A CN 201510948181A CN 105399530 A CN105399530 A CN 105399530A
- Authority
- CN
- China
- Prior art keywords
- fertilizer
- preparation
- composite microbe
- microbe fertilizer
- composite microbial
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C21/00—Methods of fertilising, sowing or planting
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
- C05F11/08—Organic fertilisers containing added bacterial cultures, mycelia or the like
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Soil Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Biochemistry (AREA)
- Environmental Sciences (AREA)
- Fertilizers (AREA)
Abstract
The invention discloses preparation and application of a composite microbial fertilizer. The preparation process concretely comprises performing liquid fermentation culturing on a bacterial strain to generate a spore, performing centrifugation, freeze-drying and grinding to prepare a bacterial powder, mixing the bacterial powder with an anti-caking powder, then performing granulation on the obtained mixture together with a granular chemical fertilizer, so as to prepare the composite microbial fertilizer. The obtained composite microbial fertilizer is applicable to culture of capsicum, pakchoi and other vegetables, and is capable of enabling microorganism to be applied to fields without changing peasant working habit, thereby realizing the purposes of improving the fertilizer utilization rate, promoting crop growth and improving crop output through effects of microorganism. Also, the composite microbial fertilizer is capable of maintaining ecological balance of soil flora, and giving play to effects of alleviating fertilizer harm and protecting environment.
Description
Technical field
The invention belongs to technical field of pesticide, be specifically related to a kind of preparation method and application of composite microbe fertilizer.
Background technology
Agricultural is to produce for the purpose of crop, and soil is the mother breeding crop, and good soil health could be achieved, the crop that cultivates.Agricultural tillage now all trends towards unit surface high yield, high crop quality, therefore the soil fertility condition of farmland whether can well-off nutrient to meet crop demand, be just primary major issue.In addition, while the quantity and quality pursuing farm crop, the fertilizer type that selection is applicable to is also proper use of, also becomes the important factor affecting farming harvest.Use chemical fertilizer in a large number for a long time, easily make soil acidification, solidification, the microbe population of soil is reduced sharply, the soil texture destroys and affects retaining and fertilizer-preserving ability, and then affects the growth of crop.And the using of excess chemical fertilizer in farm crop plantation process, make crop draw excessive chemical fertilizer, impel crop immunity degradation, crop disease-resistant and degeneration-resistant border ability also decline thereupon, cause diseases and pests of agronomic crop to be on the rise, this impels again the amount of application of chemical pesticide to increase.Cause the chemical residual amount of agricultural-food increasingly to raise thus, and the quality of agricultural-food decline thereupon.
Microbial preparation can provide crop yield and quality, enhancement soil nutrient or improvement soil physical chemistry, biological in the broadest sense, uses increase yield and quality.Common are phosphorus-solubilizing bacteria, vinelandii and root circle beneficial microorganism etc.Because biological fertilizer is with at most microbe-derived, therefore be often called " microbial fertilizer ".The advantages such as biological fertilizer has low stain, the fertile wound of nothing, amount of application is few, ecology is friendly, but it has certain storage life, and effect is more affected by environment, fertilizer efficiency is comparatively slow, uses sometimes together with chemical fertilizer, organic fertilizer.
Traditional microbiological preparation has liquid and solid two kinds of formulations.Liquid bio fertilizer is through directly cultivating or concentrated bacterium liquid.Solid biologic fertilizer is then divided into powdery or granular by the different modes of production of lyophilize thalline or absorption thalline thing.General liquid bio fertilizer can make bacterial classification in crop root fast onset effect, and its production cost is lower, but there is the problem of miscellaneous bacteria, and its storage life is not long, and qualitative change easily occurs, cause bacterium quantity decline rapidly and affect quality; The also normal intravenous extravasation and smelly of occurring in addition, and heavier, cause the upper inconvenience of transport.The solid biologic fertilizer storage life is longer, not easily qualitative change and miscellaneous bacteria occurs, bacterium number not easily fast decay and quality is more stable.But pulvis uses the upper impact by ground type, use and easily to stir up a cloud of dust afterwards, often cause peasant to use inconvenience.
Summary of the invention
The object of the present invention is to provide a kind of preparation method and application of composite microbe fertilizer; gained composite microbe fertilizer makes microorganism be applied to field under can being accustomed to not changing peasant work; thus reach through the effect of microorganism the object promoting utilization rate of fertilizer, promote plant growth, promote crop yield; and the soil bacteria group eubiosis can be safeguarded, reach the effect of protection of the environment.
For achieving the above object, the present invention adopts following technical scheme:
A kind of composite microbe fertilizer, its preparation method comprises the step of liquid state fermentation, centrifugal, freeze-drying, abrasive dust, batch mixing, granulation, and each step operation is as follows:
1) liquid fermenting: utilize fermenter, carries out fermentation culture by strain inoculation substratum after sterilization, makes it generate microbial cells, and bring out generation spore;
2) centrifugal: to utilize centrifuge to collect spore liquid state fermentation gained fermented liquid;
3) freeze-drying: adopting vacuum refrigerating machine to carry out lyophilize at-25 ~-60 DEG C in the bacterium mud containing spore of collected after centrifugation, is less than 10% to water content;
4) abrasive dust: by the bacterium block pulverizing mill pulverize after freeze-drying, its granularity is 50-70 order;
5) batch mixing: add anti-caking powder in the bacterium powder of 1:49 after abrasive dust, mix to obtain mixed powder, its bacteria containing amount is 1 × 10
9cFU/g;
6) granulation: spray anti-caking oil at the chemical fertilizer surface uniform making particle, then add mixed powder prepared by step 5) by weight 999:1 in chemical fertilizer, be prepared into particulate state further, obtain described composite microbe fertilizer.
Bacterial classification described in step 1) is phosphorus-solubilizing bacteria or bacillus mycoides; Described sterilizing comprises autoclave sterilization, steam sterilizing.
Described in step 6), anti-caking oil comprises vegetables oil, primary octadecylamine, and its consumption is that every kg chemical fertilizer sprays anti-caking oil 1.5g.
Obtained composite microbe fertilizer can adopt the cultivation of mode for vegetable crop of foliage-spray, and its consumption is 12-13kg/ mu; Described vegetable crop comprises capsicum, Plantula Brassicae chinensis.
The organic acid of phosphorus-solubilizing bacteria secretion, the former phosphate anion reactivation be fixed in soil can be made, also can discharge the positively charged ion with its bond, and this cationoid is one of trace element needed for plant simultaneously, therefore, it has obvious promoter action to the growth of leaf vegetables; Bacillus mycoides can enter in plant materials and its symbiosis, and the disease-resistant function of inducing plant, make plant can resist external pathogenic bacteria, especially effective especially to soil-borne disease.The present invention is using phosphorus-solubilizing bacteria or bacillus mycoides as bacterial classification; the spore generated after its liquid culture is joined in chemical fertilizer as microbiobacterial agent; obtained composite microbe fertilizer stability is high; microorganism is made to be applied to field under can being accustomed to not changing peasant work; thus reach through the effect of microorganism the object promoting utilization rate of fertilizer, promote plant growth, promote crop yield; and the soil bacteria group eubiosis can be safeguarded, reach the effect slowing down fertile wound, protection of the environment.Meanwhile, gained finished product convenient transportation, shelf-lives is long.
Embodiment
More being convenient to make content of the present invention understand, below in conjunction with embodiment, technical solutions according to the invention are described further, but the present invention being not limited only to this.
Embodiment 1
A kind of composite microbe fertilizer, its preparation method comprises the following steps:
1) liquid fermenting: utilize fermenter, carries out fermentation culture by phosphorus-solubilizing bacteria strain inoculation substratum upon steam sterilization, makes it generate microbial cells, and bring out generation spore;
2) centrifugal: to utilize centrifuge to collect spore liquid state fermentation gained fermented liquid;
3) freeze-drying: adopting vacuum refrigerating machine to carry out lyophilize at-25 DEG C in the bacterium mud containing spore of collected after centrifugation, is less than 10% to water content;
4) abrasive dust: by the bacterium block pulverizing mill pulverize after freeze-drying, its granularity is 50 orders;
5) batch mixing: add anti-caking powder in the bacterium powder of 1:49 after abrasive dust, mix to obtain mixed powder, its bacteria containing amount is 1 × 10
9cFU/g;
6) granulation: the amount adding 1.5g by every kg sprays one deck vegetables oil at the fertile surface uniform of the humic acid chelating making particle, then in trace element fertilizer, mixed powder prepared by step 5) is added by weight 999:1, be prepared into particulate state further, obtain composite microbe fertilizer.
Embodiment 2
A kind of composite microbe fertilizer, its preparation method comprises the following steps:
1) liquid fermenting: utilize fermenter, carries out fermentation culture by the substratum of bacillus mycoides strain inoculation after autoclave sterilization, makes it generate microbial cells, and bring out generation spore;
2) centrifugal: to utilize centrifuge to collect spore liquid state fermentation gained fermented liquid;
3) freeze-drying: adopting vacuum refrigerating machine to carry out lyophilize at-60 DEG C in the bacterium mud containing spore of collected after centrifugation, is less than 10% to water content;
4) abrasive dust: by the bacterium block pulverizing mill pulverize after freeze-drying, its granularity is 70 orders;
5) batch mixing: add anti-caking powder in the bacterium powder of 1:49 after abrasive dust, mix to obtain mixed powder, its bacteria containing amount is 1 × 10
9cFU/g;
6) granulation: the amount adding 1.5g by every kg sprays one deck primary octadecylamine at the fertile surface uniform of the humic acid chelating making particle, then in trace element fertilizer, mixed powder prepared by step 5) is added by weight 999:1, be prepared into particulate state further, obtain composite microbe fertilizer.
1. the composite microbe fertilizer that embodiment 1-2 is obtained is carried out crop (capsicum, Plantula Brassicae chinensis) pot experiment, its concrete content of the test is as follows:
1) capsicum: select that size is close, the nursery capsicum of 4 weeks, plant in 5 cun of basins that field soil equivalent volumes peat soil is housed, and process by table 1, each treatment group repeats 10.Within after field planting every 2 weeks, calculate petal quantity, fruit is gathered after transferring deep green to, record number of results, output, fruit length, capsicum plant height is measured after 3 months in test, and detect blade, fruit nitrogen content, phosphorus content, and soil available phosphorus content, it the results are shown in Table 2.
Table 1 pot experiment respectively organizes treatment process
Table 2 different treatment is to the contrast table of chili growth and its planting soil available phosphorus content
Shown by table 2 result, compared with control group, use composite microbe fertilizer of the present invention can improve available phosphorus content in soil, significantly promote chili growth, and effectively increase yield of hot pepper; , contrasted from table 2,1/2PSB and 1/2BM process is to the promoter action of plant growth higher than PSB and BM, and this may be due to a large amount fertilizer is supplied to plant in foliar spray mode, very easily causes crop to burn, damages plant on the contrary meanwhile.
2) Plantula Brassicae chinensis: select that size is close, the nursery Plantula Brassicae chinensis of 2 weeks, be planted in and be equipped with in 5 cun of boxes of field soil, and process by table 1, each treatment group repeats 10.Calibrate leaf length, Ye Kuan, plant fresh weight and the dry weight of within latter 21 days, measuring Plantula Brassicae chinensis, and after gathering, detect the content of available phosphorus in soil, it the results are shown in Table 3.
Table 3 different treatment is to the contrast table of Growth of Cabbage and its planting soil available phosphorus content
Shown by table 3 result, compared with control group, use composite microbe fertilizer of the present invention significantly can promote Growth of Cabbage; , contrasted from table 3,1/2PSB and 1/2BM process is to the promoter action of plant growth higher than PSB and BM, and this may be due to a large amount fertilizer is supplied to plant in foliar spray mode, very easily causes crop to burn, damages plant on the contrary meanwhile.
2. composite microbe fertilizer embodiment 1-2 obtained in 60 DEG C, carry out Acceleration study in the climatic chamber of humidity 75%, detect sample bacterium number after taking out and also observe physical behavior.Result shows, and gained composite microbe fertilizer of the present invention did not go bad within 2 year staging life.
3. composite microbe fertilizer obtained for embodiment 1-2 is applied to field, it can disintegration or soluble in water rapidly, and it uses convenience as seen.
The foregoing is only preferred embodiment of the present invention, all equalizations done according to the present patent application the scope of the claims change and modify, and all should belong to covering scope of the present invention.
Claims (7)
1. a preparation method for composite microbe fertilizer, is characterized in that: the step comprising liquid state fermentation, centrifugal, freeze-drying, abrasive dust, batch mixing, granulation.
2. the preparation method of composite microbe fertilizer according to claim 1, is characterized in that: described liquid state fermentation utilizes fermenter, carries out fermentation culture, make on strain inoculation substratum after sterilization it generate microbial cells, and bring out generation spore;
Described bacterial classification is phosphorus-solubilizing bacteria or bacillus mycoides.
3. the preparation method of composite microbe fertilizer according to claim 1, is characterized in that: described freeze-drying is that the bacterium mud containing spore of collected after centrifugation is carried out vacuum lyophilization in-25 ~-60 DEG C, is less than 10% to water content.
4. the preparation method of composite microbe fertilizer according to claim 1, is characterized in that: described abrasive dust be by freeze-drying after bacterium block pulverize, its granularity is 50-70 order.
5. the preparation method of composite microbe fertilizer according to claim 1, is characterized in that: described batch mixing adds anti-caking powder in the bacterium powder of 1:49 after abrasive dust, mixes to obtain mixed powder;
The bacteria containing amount of gained mixed powder is 1 × 10
9cFU/g.
6. the preparation method of composite microbe fertilizer according to claim 1, it is characterized in that: described granulation sprays anti-caking oil at the chemical fertilizer surface uniform making particle, then in chemical fertilizer, add mixed powder by weight 999:1, be prepared into particulate state further;
Described anti-caking oil comprises vegetables oil, primary octadecylamine, and its consumption is that every kg chemical fertilizer sprays anti-caking oil 1.5g.
7. the application of composite microbe fertilizer that obtains of method as claimed in claim 1, is characterized in that: adopt the mode of foliage-spray to be used for the cultivation of vegetable crop described composite microbe fertilizer, its consumption is 12-13kg/ mu;
Described vegetable crop comprises capsicum, Plantula Brassicae chinensis.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510948181.5A CN105399530A (en) | 2015-12-17 | 2015-12-17 | Preparation and application of composite microbial fertilizer |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510948181.5A CN105399530A (en) | 2015-12-17 | 2015-12-17 | Preparation and application of composite microbial fertilizer |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105399530A true CN105399530A (en) | 2016-03-16 |
Family
ID=55465339
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510948181.5A Pending CN105399530A (en) | 2015-12-17 | 2015-12-17 | Preparation and application of composite microbial fertilizer |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105399530A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110386841A (en) * | 2018-04-20 | 2019-10-29 | 联发生物科技股份有限公司 | Slow-release compound fertilizer and its method of administration |
| TWI713835B (en) * | 2018-04-20 | 2020-12-21 | 聯發生物科技股份有限公司 | Granular fertilizer and manufacturing method thereof |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1566038A (en) * | 2003-06-25 | 2005-01-19 | 中国科学院大连化学物理研究所 | Biological inorganic complex fertilizer |
| CN1830919A (en) * | 2006-03-17 | 2006-09-13 | 昆明榕风生物技术有限公司 | Environmental protection type synergia and controlled-releasing fertilizer and its prepn. method |
| CN102976841A (en) * | 2011-09-06 | 2013-03-20 | 重庆市万植巨丰生态肥业有限公司 | Method for producing composite microbial fertilizer by using coating technology |
| KR20140146789A (en) * | 2013-06-18 | 2014-12-29 | (주)한국농축산기술 | System for total producing high speed and high quality amino acids fertilizers and feeds, using the slaughtered livestock's blood protein , and the producing method thereof |
| CN104341235A (en) * | 2013-07-31 | 2015-02-11 | 联发生物科技股份有限公司 | Method for preparing biological fertilizer bacterium granules |
-
2015
- 2015-12-17 CN CN201510948181.5A patent/CN105399530A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1566038A (en) * | 2003-06-25 | 2005-01-19 | 中国科学院大连化学物理研究所 | Biological inorganic complex fertilizer |
| CN1830919A (en) * | 2006-03-17 | 2006-09-13 | 昆明榕风生物技术有限公司 | Environmental protection type synergia and controlled-releasing fertilizer and its prepn. method |
| CN102976841A (en) * | 2011-09-06 | 2013-03-20 | 重庆市万植巨丰生态肥业有限公司 | Method for producing composite microbial fertilizer by using coating technology |
| KR20140146789A (en) * | 2013-06-18 | 2014-12-29 | (주)한국농축산기술 | System for total producing high speed and high quality amino acids fertilizers and feeds, using the slaughtered livestock's blood protein , and the producing method thereof |
| CN104341235A (en) * | 2013-07-31 | 2015-02-11 | 联发生物科技股份有限公司 | Method for preparing biological fertilizer bacterium granules |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110386841A (en) * | 2018-04-20 | 2019-10-29 | 联发生物科技股份有限公司 | Slow-release compound fertilizer and its method of administration |
| TWI713835B (en) * | 2018-04-20 | 2020-12-21 | 聯發生物科技股份有限公司 | Granular fertilizer and manufacturing method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102030581B (en) | Novel multi-functional biological organic seed dressing agent and method for preparing same | |
| CN104560828A (en) | Microbial agent and application in prospect of passivating heavy metals of soil | |
| CN105316249B (en) | A kind of bacillus subtilis and microbial bacterial agent and their applications in fermentation maturity | |
| CN102826879B (en) | Production method of active microbial organic fertilizer | |
| CN103964946A (en) | Special compound microbial fertilizer for paddy rice and preparation method thereof | |
| CN107297388B (en) | A kind of soil microenvironment restorative procedure for eliminating continuous cropping obstacle | |
| CN104255420B (en) | A kind of seedling medium of melon diseases prevention growth promoting and preparation method thereof | |
| CN105237141B (en) | A kind of straw biological matrix seedling-cultivation plate and preparation method thereof | |
| CN104496573B (en) | A kind of grape special biological and ecological methods to prevent plant disease, pests, and erosion composite microbiological fertilizer, production method and application | |
| CN106047764A (en) | Low-temperature beneficial microorganism preparation product applied to straw application | |
| CN104560045A (en) | Microbial agent and improvement application of saline alkali and desertification soil caused by excessive use of fertilizer | |
| CN105294277A (en) | Light matrix formula for camellia oleifera seedlings and preparation method thereof | |
| CN106190929B (en) | A kind of new strain of Bacillus licheniformis and its application | |
| CN108887142A (en) | A kind of full nutrition organic ecological soilless culture substrate formula suitable for cucumber | |
| CN107012105A (en) | Composite bacteria agent, animals died of illness harmless treatment fermenting agent, composite microbe fertilizer, solid medium and fluid nutrient medium | |
| CN106007824B (en) | Composite bacterial fertilizer and preparation method and application thereof | |
| CN105622296A (en) | Composite soil modifying agent and preparation method and application thereof | |
| CN107324926A (en) | Suitable for the improving fertilizer and its production method in salt-soda soil | |
| CN103524220A (en) | Production method for rice seedling cultivation dedicated organic fertilizer | |
| CN105418240A (en) | Salt-alkali-resistant cow dung organic fertilizer containing panax notoginseng saponins | |
| CN108610182A (en) | A kind of compound fertilizer and preparation method thereof with prophyiaxis and promoting growth function | |
| CN105399530A (en) | Preparation and application of composite microbial fertilizer | |
| CN102515897A (en) | Dry cow dung powder organic fertilizer, preparation method thereof and application thereof | |
| CN107721539A (en) | A kind of samphire is special to mix bacterial manure and preparation method | |
| CN102942409A (en) | Humic acid nutrient-gathering fertilizer and production method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20160316 |
|
| RJ01 | Rejection of invention patent application after publication |