CN105349449A - ECMA (ecols composite microbial agents) preparation method - Google Patents
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- CN105349449A CN105349449A CN201510605630.6A CN201510605630A CN105349449A CN 105349449 A CN105349449 A CN 105349449A CN 201510605630 A CN201510605630 A CN 201510605630A CN 105349449 A CN105349449 A CN 105349449A
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Abstract
The invention discloses an ECMA (ecols composite microbial agents) preparation method. The method includes: inoculating purified penicillia to a slope of a potato culture medium, culturing, inoculating to a shake flask culture medium, performing shake-flask shake culture, inoculating to a liquid seed culture medium, culturing for 36-54 hours, sprinkling and inoculating to a solid fermentation medium, culturing at the temperature of 26-37 DEG C for 136-168 hours with a ventilation rate being 1:0.2-0.8, compounding prepared penicillium spore powder with bacillus subtilis to obtain a composite agriculatural microbial agent with functions of phosphate solubilizing and generating of various physiological activators. The utilization rate of phosphate in soil can be increased, soil-borne diseases can be prevented and reduced, and the efficacies of yield increasing and revenue increasing are achieved.
Description
Technical field
The invention belongs to bioengineering field technology, relate to the manufacture method of ECMA complex micro organism fungicide.
Background technology
In order to solve the demand of population growth to output, in agriculture production, long-term dependence uses chemical fertilizer, agricultural chemicals as the major measure of volume increase in a large number, so green manure, farm manure are out in the cold, the usage quantity of chemical fertilizer gets more and more, agriculture production cost increases sharply, also cause soil compaction, fertilizer declines simultaneously.Due to the loss of chemical fertilizer, agricultural chemicals, residual, have impact on quality of agricultural product and HUMAN HEALTH, cause environmental pollution, also destroy original microecological balance in crop rhizosphere soils simultaneously, soil-borne disease is increased the weight of.Soil is crop growthing development nutrition source, and it is made up of mineral substance, organism and microorganism three parts.Soil mineral is the main body of soil material composition, and being the mechanism of plant-growth, is again the main source of plant inorganic nutrients.Organic matter is the important basic substance of fertilizer provision from soil, fertilizer conservation, is again the important neccessary composition forming soil transferring structure and keep soil from packing together.Microorganism is the organism of living in soil, is to transform the indispensable active substance of soil fertility.Soil mineral, relation between organism and microorganism three just look like the relation of the skeleton of animal, muscle and blood cell, and microorganism is wherein most active integral part.The conversion of soil, matter and energy is participated in the activity of soil microorganisms directly.The soil fertility such as formation Sum decomposition, nutrients release, N immobilization of soil ulmin is formed and growth course.Therefore, in soil, enrich quantity of useful microbe also can strengthen soil microbial activities, increase soil fertility.Soil microbial activities particularly around farm crop rhizosphere is improved even more important to root system of plant nutrient environment, and using Penicillium notatum spore powder, bacillus subtilis etc. at rhizosphere can increase useful mushroom quantity and effect in root layer soil.Agricultural basis at soil, microorganism be soil genesis and development enliven factor.In recent years, the popularization of home farm and association, people are made more to pay attention to microorganism fertilizer improvement soil, improve the active effects such as plant nutrition absorptive function, along with the continuous popularization of Quality-oriented Agriculture, harmless organic agricultural-food will become the trend of the future of agriculture development, and functional microbial fertilizer is just playing maintenance high crop yield, is setting up the effects such as good ecotope, is the effective guarantee of development quality agricultural.。
Summary of the invention
The present invention is to provide the production method of ECMA complex micro organism fungicide, present inventor finds amino acid granular in Penicillium notatum substratum as nitrogenous source under study for action, effect significantly, and use with amino acid granular be the useful promotion of Penicillium notatum composite microbial manure to the process of growth of paddy rice, seeding corn and other crops of nutrient carriers, the effect of increasing income significantly.And bacillus subtilis (BY1336) microorganism is to the useful promotion of the process of growth of paddy rice, seeding corn and other crops, and for the soil that continuous cropping is planted, prevent and treat soil-borne disease Be very effective.
The present invention uses Penicillium notatum, and vegetative mycelium is colourless, and branch becomes the conidium of broom shape to stretch in the air from mycelium, and the stigma on each top produces the dark green conidium of chain, conidium smooth surface.Produce the various plants growth regulatory substances such as phytokinin, indolylacetic acid, Plant hormones regulators,gibberellins during this bacterium metabolism, also produce the active substance of Multiple Classes of Antibiotics class.
The present invention realizes in the following manner: 1, the manufacture method of ECMA complex micro organism fungicide, it is characterized in that the method is carried out in the steps below:
(1) slant strains is cultivated: in the test tube that potato culture is housed, inoculating strain Penicillium notatum (the Heilungkiang agriculture company limited of a pleasure provides), 26 DEG C ~ 34 DEG C slant culture one week, until the sorus that gas is given birth to is dark green;
(2) shaking flask bacterium culture medium and spawn culture:
Substratum: glucose 10 ~ 50g/l, KH
2pO
40.5 ~ 2g/l, K
2hPO
40.5 ~ 3g/l, (NH
4)
2sO
41 ~ 4g/l, MgSO
47H
2o0.2g/l, corn steep liquor 0.5 ~ 3g/l, CaCO
35 ~ 20g/l, regulates pH to 7.0 ~ 8.0;
In the triangular flask that shaking flask bacterium culture medium is housed, inoculate above-mentioned slant culture bacterial classification, on 150 revs/min of shaking tables, 26 ~ 37 DEG C, cultivate 20-28 hours;
(3) liquid fermentation tank seed culture medium and enlarged culturing:
Substratum: glucose 10 ~ 200g/l, KH
2pO
41 ~ 2g/l, K
2hPO
41 ~ 3g/l, (NH
4)
2sO
42 ~ 6g/l, MgSO
47H
2o0.2g/l, amino acid granular 1 ~ 10g/l, pH value is 5.5 ~ 8.5, leavening temperature 28 ~ 40 DEG C, ventilating ratio 0 ~ 1.0vvm, and mixing speed is 20 ~ 500rpm, and incubation time is 36 ~ 54 hours.
(4) solid fermentation:
Substratum: cavings or rice chaff 35 ~ 55%, starch 8 ~ 16%, amino acid granular 4 ~ 10%, wheat bran 0.5 ~ 2%, rock phosphate powder 10 ~ 15%, peat composed of rotten mosses ash or straw powder 20 ~ 30%, to add water 25 ~ 40%, pH6.0 ~ 8.0 by the solid fermentation substratum of this proportions.
Inoculation fermentation tank seed liquor in the solid culture case that above-mentioned substratum is housed; Inoculum size is 5 ~ 10%, cultivates at 26 ~ 37 DEG C, and ventilation is 1:0.2 ~ 0.8, ferments 136 ~ 168 hours, after producing spore, obtains described bulk or powdery product 55 ~ 80 DEG C of oven dry.
(5) Homogeneous phase mixing:
The powdery product above-mentioned 1-4 steps made makes ECMA after mixing by the ratio uniform of 1:1 with bacillus subtilis (bacterial classification BacillussubtilisY1336 is to be derived from the safe biotechnology company in Taiwan hundred) microbial preparation.
This ECMA complex micro organism fungicide contains 200,000,000/gram more than to live spore Penicillium notatum and bacillus subtilis (BY1336), meet agricultural microbial agent standard.
GB20287-2006 " agricultural microbial agent " is adopted to specify that middle plateform counting process detects, the obtained ECMA complex micro organism fungicide of aforesaid method can reach 200,000,000/gram more than to live spore Penicillium notatum and bacillus subtilis (BY1336), can reach 1,000,000,000/gram work spore Penicillium notatum and bacillus subtilis (BY1336).
This ECMA complex micro organism fungicide can be used as base fertilizer to agricultural crops with fertilizer, inorganic fertilizer blending and topdress.Base applying method is every mu of use 0.8-2.6 kilograms, is attached on fertilizer, inorganic fertilizer, carries out spreading manuer in holes or ditch spread.
The bio-fertilizer dosing ECMA complex micro organism fungicide has following positively effect:
This kind of microbial inoculum produces a large amount of cellulases in soil, and this kind of enzyme participates in the decomposition course of the soil organism directly, the carbon compound of complexity can be decomposed into simple compound, thus can ensure the absorption of plant to organic nutrient.
Interior life and ectomycorrhiza can be formed with root system of plant.The acid phosphatase of mycorhiza secretion, has a significant effect to organic and inorganic state phosphoric acid salt in dissolving soil, thus adds the storage capacity intensity of rhizosphere soil available phosphorus, meet plant to phosphorus nourishing demand quantitatively.
Several physiological active substances can be produced, such as phytokinin, indolylacetic acid, Plant hormones regulators,gibberellins etc.These physiologically active substances, can significantly improve the rooting ability of plant and the receptivity to some middle and trace element, and Promoting plant growth is grown, and increase the anti-adversities such as drought resisting, disease-resistant, Salt And Alkali Tolerance.
The meta-bolites produced, effectively can suppress the growth and reproduction of pathogenic bacteria and virus in soil, and purification soil, alleviates the generation of soil-borne disease.
Using multiple organic and inorganic material as bio-carrier, and can be changed into by organic carrier in 35 days " the green nutrient " that can be absorbed by plants, this produces Development of Organic Agriculture and green product, and tool is of great significance.
ECMA complex micro organism fungicide of the present invention has the following advantages:
1, improve soil: after using ECMA complex micro organism fungicide of the present invention, the beneficial microorganism on the one hand in ECMA at crop root amount reproduction, and produces a large amount of secondary metabolites in reproductive process, so just can promote the formation of soil aggregate.The soil hardened is become loose, makes its fertilizer conservation, water-retentivity and venting capability strengthen as farm crop provide comfortable growing environment simultaneously by the crumb structure formed in soil, therefore can promote growing of root system.
2, anti-continuous cropping: be manured into soil by ECMA complex micro organism fungicide of the present invention, because it contains a large amount of beneficial microorganisms, therefore can make crop obtain the nutrition of comparing general equilibrium, add the disease resistance of crop, therefore serve the effect of anti-continuous cropping.
3, strong stress resistance (cold-resistant, drought resisting, disease resistance): ECMA complex micro organism fungicide of the present invention is in soil, mycorhiza can be impelled to secrete a large amount of physiologically active substances, such as: phytokinin, indolylacetic acid, Plant hormones regulators,gibberellins etc., the rooting ability of plant and the receptivity to some middle and trace element can be significantly improved, stimulate and Promoting plant growth growth, strengthen root of hair ability, improve nutritive ingredient absorption intensity, regulating plant physiological metabolism, increases the anti-adversities such as drought resisting, disease-resistant, Salt And Alkali Tolerance.ECMA complex micro organism fungicide of the present invention can provide comprehensive and the nutrition of equilibrium to crop, and then improves the resistivity of plant to various pathogenic bacterium greatly.
4, the use of chemical fertilizer and agricultural chemicals is significantly reduced: because the various trace elements contained in ECMA complex micro organism fungicide of the present invention can constitute a nutritive equilibrium system with macroelement such as nitrogen, phosphorus, potassium, thus promote that plant is to the absorption of nitrogen, phosphorus, potassium, allow crop obtain balanced nutritious, therefore can reduce use chemical fertilizer.If coordinate the use of biological organic fertilizer even can not use chemical fertilizer.Comprehensive nutrition makes plant growth healthy and strong not easily sick, in addition in ECMA complex micro organism fungicide of the present invention can add complexing agent, physiologically active that activator, spreader-sticker can strengthen agricultural chemicals, agricultural chemicals can also be increased in the adhesion strength of blade and time, therefore improve the drug effect of agricultural chemicals.Not easily sick on the one hand, improve its result of drug effect on the other hand and obviously can reduce pesticide dosage exactly.The basis obviously reducing chemical fertilizer, pesticide dosage being changed and uses biological organic fertilizer and biological pesticide, is very favourable for production organic farm products.
5, the quality of agricultural-food, taste is increased substantially; ECMA complex micro organism fungicide of the present invention can meet the balanced in nutrition of crop, and its growth regularity by self is grown, and grows normal, and metabolism is steady, can improve the quality of agricultural-food in the nature of things, taste, recovers that it is natural flavour mountaineous.
6, the effect of increasing income is obvious: the microorganism that ECMA complex micro organism fungicide of the present invention contains is in the process of soil Germination and growth, the material of a large amount of useful plants growth can be secreted, these material incentive crop root growth are flourishing, thus the nutritive element that can absorb needed for more crops, and without antagonism between each element, crop fertilization is given with it, crop to various nutrition can comprehensively and the ground of equilibrium absorb, there is not obstruction factor, minimum nutrient, thus also just can show maximum yield level.
7, ECMA gives birth to and ectomycorrhiza in being formed with plant, and can secrete multiple enzyme, these enzymes have significant effect to the degraded of organic compound in soil and the phosphatic activation of the state that is fixed, and can significantly improve the nutrient storage capacity of arable soil:
1. ECMA can produce multiple meta-bolites in soil, and these materials, to the growth and reproduction suppressing pathogenic bacteria in soil, have a significant effect, thus reaches purification soil, minimizing soil disease generation object.
2. after ECMA enters soil, a large amount of cellulases can be secreted, within a short period of time, by the macromole carbon compound of complexity, simple micromolecular compound can be decomposed into, thus provide sufficient organic nutrient for plant.
3. after ECMA enters soil, can secrete a large amount of Phosphoric acid esterases in soil, this kind of enzyme has a significant effect to the phosphoric acid salt dissolving in soil the state that is fixed, and can provide abundant phosphorus nourishing for plant, reduces phosphate fertilizer fixing in soil.
8, ECMA can improve crop yield, improves crop quality, reduces chemical fertilizer input, reduces production cost, improve edatope, is that Organic farming plants first-selected microbial preparation.Fertilizer efficiency period is long, and under normal circumstances, biological effect can reach 3-5 months.There is good dormant trait, under drying, ventilation condition, can 3-5 years be preserved, and not lose biological activity.Be in the ECMA pulvis of dormant state, can coexist with various inorganic salts, sterilant, sterilant; Film or puddle in various inorganic complex fertilizer, there will not be high density inorganic salt to biological lethality.Compared with zymocyte liquid, ECMA spore pulvis, in all kinds of granulation of fertilizer technique, has stronger high thermal resistance.When temperature, humidity reach suitable condition, ECMA can fast-germination in soil.
9, special efficacy is had to chilling injury, freeze injury: early spring, weather was abnormal, chilling injury, frost, cold spell in later spring, and heavy snow etc. have harm to farm crop.Fruits and vegetables, melon nursery stage, setting date, once occur freeze injury, damage to plants caused by sudden drop in temperature, as long as vegetative point is not also freezed to death, ECMA just can save.
10, degraded toxic gas: the ozone in low latitude, carbon monoxide, sulfurous gas, the toxic gases such as acid rain are very large to the injury of plant, are difficult to prevention.Farm crop, once there be blade face to turn to be yellow after contaminated, will soon wither.ECMA can make crop recover normal as early as possible, comes back to life.
Embodiment
Illustrate further the present invention by example below, and these enforcements do not limit the scope of the invention.
In following instance:
Bacterial strain: Penicillium notatum, bacillus subtilis (BY1336)
Slant culture based formulas: potato-glucose sugar nutrient agar
Amino acid granular: be the industrial waste after fermented gournet powder liquid extracts monosodium glutamate and tropina, through concentrated and that spray granulating is obtained byproduct, total nutrient >=12%, organic >=35%, it is good bacterium powder carrier, for the sprouting of spore in soil provides important nutrients, and can supply plant slowly-releasing organic nitrogen source.
Embodiment 1
The first step, slant culture.Inoculating strain Penicillium notatum on slant medium after sterilization, then puts into these test tubes at 28 DEG C of slant culture in incubator, incubation time one week, until the sorus of gas life is dark green.
Second step, shaking flask spawn culture.
Used medium is filled a prescription: glucose 30g/l, KH
2pO
46g/l, K
2hPO
42.3g/l, (NH
4)
2sO
43g/l, MgSO
47H
2o0.2g/l, corn steep liquor 2g/l, regulate pH to 7.5.
In the 500ml triangular flask that shaking flask bacterium culture medium is housed, liquid amount is 100ml, inoculates above-mentioned slant culture bacterial classification, on 150 revs/min of shaking tables, 30 DEG C, cultivates 26 hours.
3rd step, liquid fermentation tank seed culture.
Used medium is filled a prescription: glucose 120g/l, KH
2pO
42g/l, K
2hPO
42.5g/l, (NH
4)
2sO
45g/l, MgSO
47H
2o0.2g/l, amino acid granular 8g/l, pH value is 7.5, in 500 liters of fermentor tanks, preparing 350 liters of substratum.Leavening temperature 32 DEG C, ventilating ratio 0.8vvm, mixing speed is 200rpm, and incubation time is 48 hours.
4th step, solid fermentation.
Solid fermentation culture medium prescription (weight ratio): cavings 40%, starch 12%, amino acid granular 8%, wheat bran 1.5%, rock phosphate powder 12%, straw powder 26.5%, another amount of water is 30%, pH8.0 of above substratum total amount.Cavings, amino acid granular, rock phosphate powder, wheat bran, straw powder need to pulverize, and reach 70-90 orders.
Adopt 1 cubic metre of solid culture case, after configuration substratum completes, sterilizing is stand-by.Fermentor tank seed liquor inoculum size is 10% of solid fermentation substratum, fully stirs after inoculation, its material is inoculated evenly, cultivates at 32 DEG C, and note ventilating, ventilation is expressed as 1:0.5 with volume/volume, ferments 140 hours, stops fermentation.
5th step, dries
Use low temperature air flow drying machine to dry, bake out temperature 75 DEG C, control water-content and reach < 5%, obtain qualified product like this, this product meets agricultural microbial agent standard.
6th step Homogeneous phase mixing:
The powdery product above-mentioned 1-5 steps made makes ECMA after mixing by the ratio uniform of 1:1 with bacillus subtilis (bacterial classification BacillussubtilisY1336 is to be derived from the safe biotechnology company in Taiwan hundred) microbial preparation.
Embodiment 2
The first step, slant culture.Inoculating strain Penicillium notatum on slant medium after sterilization, then puts into these test tubes at 30 DEG C of slant culture in incubator, incubation time one week, until the sorus of gas life is dark green.
Second step, shaking flask spawn culture.
Used medium is filled a prescription: glucose 35g/l, KH
2pO
41g/l, K
2hPO
41.33g/l, (NH
4)
2sO
44g/l, MgSO
47H
2o0.2g/l, corn steep liquor 2.5g/l, regulate pH to 7.2.
In the 500ml triangular flask that shaking flask bacterium culture medium is housed, liquid amount is 100ml, inoculates above-mentioned slant culture bacterial classification, on 150 revs/min of shaking tables, 32 DEG C, cultivates 26 hours.
3rd step, liquid fermentation tank seed culture.
Used medium is filled a prescription: glucose 100g/l, KH
2pO
41.5g/l, K
2hPO
42g/l, (NH
4)
2sO
46g/l, MgSO
47H
2o0.2g/l, amino acid granular 10g/l, pH value is 8.0, in 500 liters of fermentor tanks, preparing 350 liters of substratum.Leavening temperature 34 DEG C, ventilating ratio 0.8vvm, mixing speed is 200rpm, and incubation time is 48 hours.
4th step, solid fermentation.
Solid fermentation culture medium prescription (weight ratio): rice chaff 42%, starch 12%, amino acid granular 10%, wheat bran 1.5%, rock phosphate powder 12%, peat composed of rotten mosses ash 22.5%, another amount of water is 30%, pH8.0 of above substratum total amount.Cavings, amino acid granular, rock phosphate powder, wheat bran, straw powder need to pulverize, and reach 70-90 orders.
Adopt 1 cubic metre of solid culture case, after configuration substratum completes, sterilizing is stand-by.Fermentor tank seed liquor inoculum size is 8% of solid fermentation substratum, fully stirs after inoculation, its material is inoculated evenly, cultivates at 35 DEG C, and note ventilating, ventilation is expressed as 1:0.8 with volume/volume, ferments 158 hours, stops fermentation.
5th step, dries
Use low temperature air flow drying machine to dry, bake out temperature 65 DEG C, control water-content and reach < 10%, obtain qualified product like this, this product meets agricultural microbial agent standard.
6th step, Homogeneous phase mixing:
The powdery product above-mentioned 1-5 steps made makes ECMA after mixing by the ratio uniform of 1:1 with bacillus subtilis (bacterial classification BacillussubtilisY1336 is to be derived from the safe biotechnology company in Taiwan hundred) microbial preparation.
Embodiment 3
1 ton of amino acid granular blending, 20 kilograms of ECMA, make amino acid biological fertilizer, carry out organic rice planting experiment, amino acid biological fertilizer and inorganic fertilizer controlled trial see the following form.Plantation result shows, disposable mu executes amino acid biological fertilizer 30kg, does not chase after any fertilizer between breeding time, and still can ensure that robust plant, well developed root system, color and luster are dark green, not de-fertile, yield level remains basically stable compared with conventional fertilizer application.
Amino acid biological fertilizer is on the impact (2014) of organic paddy rice output
Organic food paddy rice Ce Chan unit: kg/ ㎡
| Process | On average | Per mu yield | |||
| Biological organic fertilizer | 0.85 | 0.89 | 0.9 | 0.87 | 582.5 |
| " 733 " fertilizer | 0.82 | 0.80 | 0.83 | 0.817 | 544.7 |
Illustrate: field actual measurement output, 3 points are adopted in every treatment zone, often 1 ㎡, and threshing is surveyed.
Survey product result to show: rice application amino acid biological fertilizer, under not prerequisite to fertilize, still higher output is obtained, triadic mean per mu yield 582.5kg, contrast with " 733 " fertilizer per mu yield 544.7kg, mu volume increase 37.8kg, amount of increase in production reaches 7.0%, this is that Organic food rice cropping obtains higher output, provides a kind of new and effective Eco-fertilizer kind.From test-results, advantageously, organic paddy rice per mu yield is equally matched with chemical fertilizer per mu yield up to 582.5kg for amino acid biological fertilizer, for green organic food cultivation realizes high-quality, highly efficient and productively to take a firm foundation.
Amino acid biological fertilizer is on the impact (2013) of organic paddy rice output
The amino acid granular related in component of the present invention is the byproduct being produced in the process of monosodium glutamate the gained that ferments by the biochemical energy (Longjiang) company limited of China Oil and Food Import and Export Corporation.
The Penicillium notatum related in component of the present invention is penicillium oxalicum, derives from Chinese agriculture Microbiological Culture Collection administrative center.
The bacillus subtilis (bacterial classification BacillussubtilisY1336) related in component of the present invention is derived from the safe biotechnology company in Taiwan hundred.
Claims (7)
- The manufacture method of 1.ECMA complex micro organism fungicide, is characterized in that the method is carried out in the steps below:(1) slant strains is cultivated: in the test tube that potato culture is housed, inoculating strain Penicillium notatum (the Heilungkiang agriculture company limited of a pleasure provides), 26 DEG C ~ 34 DEG C slant culture one week, until the sorus that gas is given birth to is dark green;(2) shaking flask bacterium culture medium and spawn culture:Substratum: glucose 10 ~ 50g/l, KH 2pO 40.5 ~ 2g/l, K 2hPO 40.5 ~ 3g/l, (NH 4) 2sO 41 ~ 4g/l, MgSO 47H 2o0.2g/l, corn steep liquor 0.5 ~ 3g/l, CaCO 35 ~ 20g/l, regulates pH to 7.0 ~ 8.0;In the triangular flask that shaking flask bacterium culture medium is housed, inoculate above-mentioned slant culture bacterial classification, on 150 revs/min of shaking tables, 26 ~ 37 DEG C, cultivate 20-28 hours;(3) liquid fermentation tank seed culture medium and enlarged culturing:Substratum: glucose 10 ~ 200g/l, KH 2pO 41 ~ 2g/l, K 2hPO 41 ~ 3g/l, (NH 4) 2sO 42 ~ 6g/l, MgSO 47H 2o0.2g/l, amino acid granular 1 ~ 10g/l, pH value is 5.5 ~ 8.5, leavening temperature 28 ~ 40 DEG C, ventilating ratio 0 ~ 1.0vvm, and mixing speed is 20 ~ 500rpm, and incubation time is 36 ~ 54 hours.(4) solid fermentation:Substratum: cavings or rice chaff 35 ~ 55%, starch 8 ~ 16%, amino acid granular 4 ~ 10%, wheat bran 0.5 ~ 2%, rock phosphate powder 10 ~ 15%, peat composed of rotten mosses ash or straw powder 20 ~ 30%, to add water 25 ~ 40%, pH6.0 ~ 8.0 by the solid fermentation substratum of this proportions.Inoculation fermentation tank seed liquor in the solid culture case that above-mentioned substratum is housed; Inoculum size is 5 ~ 10%, cultivates at 26 ~ 37 DEG C, and ventilation is 1:0.2 ~ 0.8, ferments 136 ~ 168 hours, after producing spore, obtains described bulk or powdery product 55 ~ 80 DEG C of oven dry.(5) Homogeneous phase mixing:The powdery product above-mentioned 1-4 steps made makes ECMA after mixing by the ratio uniform of 1:1 with bacillus subtilis (bacterial classification BacillussubtilisY1336 is to be derived from the safe biotechnology company in Taiwan hundred) microbial preparation.
- 2. the manufacture method of Penicillium notatum spore powder according to claim 1, is characterized in that in described shaking flask spawn culture based formulas, each moiety all can in proportion for cultivating within the scope of it; Its optimum formula is glucose 30g/l, KH 2pO 42g/l, K 2hPO 42.3g/l, (NH 4) 2sO 43g/l, MgSO 47H 2o0.2g/l, corn steep liquor 2g/l and control condition are 30 DEG C and cultivate 26 hours.
- 3. the manufacture method of Penicillium notatum spore powder according to claim 1,It is characterized in that in described fermentor tank seed culture based formulas, each moiety all can in proportion for cultivating within the scope of it; Its optimum formula is glucose 120g/l, KH 2pO 42g/l, K 2hPO 42.5g/l, (NH 4) 2sO 45g/l, MgSO 47H 2o0.2g/l, amino acid granular 8g/l and be pH value 7.5 in control condition, leavening temperature 32 DEG C, ventilating ratio 0.8vvm, mixing speed is 200rpm, and incubation time is 48 hours.
- 4. the manufacture method of Penicillium notatum spore powder according to claim 1,It is characterized in that in described fermentor tank seed culture based formulas, each moiety all can in proportion for cultivating within the scope of it; Its best solid fermentation culture medium prescription (weight ratio) is cavings or rice chaff 40%, starch 12%, amino acid granular 8%, wheat bran 1.5%, rock phosphate powder 12%, peat composed of rotten mosses ash or straw powder 25%, adds water 30% by the solid fermentation substratum of this proportions.
- 5. the manufacture method of Penicillium notatum spore powder according to claim 1, it is characterized in that the add-on of described fermentor tank seed liquor is 5 ~ 10% of solid fermentation weight of material, its optimal addn is 8% of solid fermentation weight of material.
- 6. the Penicillium notatum spore powder that method manufactures according to claim arbitrary in claim 1-5, it is characterized in that this product contain 200,000,000/gram more than to live spore Penicillium notatum.
- 7. the Penicillium notatum spore powder that method manufactures according to claim arbitrary in claim 1-5, is characterized in that to use with amino acid granular blending, can reach increasing crop yield the effect of increasing income.
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|---|---|---|---|---|
| CN107760610A (en) * | 2017-09-29 | 2018-03-06 | 中国科学院南京土壤研究所 | One plant long spore Mortierella and its application |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102134168A (en) * | 2010-01-26 | 2011-07-27 | 东莞市保得生物工程有限公司 | Microbial foliage synergist |
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102134168A (en) * | 2010-01-26 | 2011-07-27 | 东莞市保得生物工程有限公司 | Microbial foliage synergist |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107760610A (en) * | 2017-09-29 | 2018-03-06 | 中国科学院南京土壤研究所 | One plant long spore Mortierella and its application |
| CN107760610B (en) * | 2017-09-29 | 2021-01-19 | 中国科学院南京土壤研究所 | Mortierella elongata and application thereof |
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