CN1053320C - 脱脂乳的胶束酪蛋白的用途 - Google Patents
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Abstract
本发明是关于含胶束酪蛋白的防龋食用组合物。将胶束酪蛋白与不同于酪蛋白的乳成分按与该乳中所遇到的相类似的比例混合，而胶束酪蛋白则是通过动物乳的微量过滤，超滤和/或透滤法获得。
Description
本发明涉及脱脂乳的胶束酪蛋白的用途。
某些酪蛋白衍生物公知具防龋特性。
因此，US 5015628(墨尔本大学)描述了一种防龋组合物，它具体含例如由α或β酪蛋白的胰蛋白酶水解所得的磷肽(phosphopeptide)作活性剂。
US 4994441(Nestec S.A.)描述了防斑和防龋组合物，在该组合物中，活性剂选自κ-酪蛋白糖肽(kappa-caseinoglycopeptides)及其脱唾液酰化的(desialylated)衍生物。
关于该相同的主题，FR 2672494(Dr.Madinier)描述了有助于预防龋牙的预防酶(prophylactic ferments)。这些酶可被单独使用或者与用于生产例如酸奶的酶配合使用。
本发明的目的是提供含基本上是天然形式的活性剂的防龋食用组合物。
为此，本发明防龋食用组合物包含有效量的胶束酪蛋白。
事实上令人惊讶的是，已发现酪蛋白本身若以胶束形式存在则显示显著的防龋活性。
本发明也涉及有效量胶束酪蛋白在生产防龋食用组合物方面的用途。
在本发明组合物中或就本发明的用途而言，胶束酪蛋白可以来自例如动物乳，如牛乳、山羊乳或绵羊乳。
该胶束酪蛋白可以例如通过动物乳的微量过滤(microfiltration)、超滤和/或透过过滤(diafiltration)来获得。
胶束蛋白最好通过使动物乳通过无机膜的微量过滤来获得，所述无机膜的孔隙度例如约为0.1-0.2μm。
在本发明组合物中或就本发明用途而言的一个优选的实施方案中，将不同于酪蛋白的动物乳的成分按与动物乳中的相类似的比例加至胶束酪蛋白中或与胶束酪蛋白混合。
以下实施例是用来举例说明上述的组合物和本发明的用途并用实验说明上述的防龋特性。
在这些实施例中，除非另有说明，百分数及份数均以重量计。实施例1，对比实施例i)-iii)
制备4个组合物，其中之一符合本发明，而另外3个用作对比。每个组合物含30％蔗糖、32％面粉、5％酒酵母和2％微量养料(Gevral混合物)，剩下的30％由下列成分构成：实施例1：工业用脱脂牛奶粉对比实施例i)：基于酪蛋白酸盐的脱脂牛奶粉的替代物，它包含：
酪蛋白酸钠 41.9％
乳糖 49.1％
Ca3(C6H5O7)2.4H2O 2.3％
CaCl2 1.8％
MgCl2·6H2O 0.7％
K2HPO4.3H2O 4.2％对比实施例ii)：基于乳清的脱脂牛奶粉的替代物，它包含：
乳清蛋白提浓物(80％蛋白) 45.4％
乳糖 45.3％
Ca3(C6H5O7)2.4H2O 2.0％
CaCl2 1.5％
MgCl2.6H2O 0.6％
K2HPO4.3H2O 3.4％
Na2HPO4 1.6％
组氨酸.HCl-H2O 0.2％对比实施例iii)：脱脂牛奶粉的大豆蛋白替代物，它包含：
大豆蛋白提取物(91％蛋白) 39.3％
乳糖 49.3％
Ca3(C6H5O7).4H2O 2.4％
CaCl2 1.9％
MgCl2.6H2O 0.5％
K2HPO4.3H2O 4.3％
Na2HPO4 1.9％
L-甲硫氨酸 0.3％
L-赖氨酸.HCl 0.1％
使用六窝(每窝4只)刚出生的Osborne-Mendel大鼠。在它们出生后的第13天，将这些大鼠和它们的母亲转移至带栅格板的不锈钢笼中，按需饲喂Nafag提浓物颗粒和自来水直至它们出生后的第20天。然后将这些大鼠随机分成6组，每组大鼠用上述的四种组合物之一饲喂并按需给自来水。
在第21和22天，给每个大鼠100μl Streptococcus sobrinus(0MZ-176)和Actinomyces viscosus(Ny-1)的浓悬浮液，每天两次。
在第40天，用常规方法测定牙斑情况、龋[初裂(incipientfissures)、深裂(advanced fissures)和光滑表面的龋]数及增重。结果见下表I。
在表I中，牙斑的大小用0-4标记，0表示无斑，4则表示最大斑。初裂和深裂数的最大值是12。光滑表面龋数的最大值为20。增重用g表示。表I实施例序号 牙斑 初裂 深裂 光滑表面龋 增重1 2.8 7.1 2.7 6.2 105i) 2.5 9.0 4.9 13.6 102ii) 2.0 7.8 3.9 12.3 96iii) 2.3 9.5 6.1 12.3 102
可以看出，最好的食物无疑是给予大鼠的实施例1组合物，该组合物含得自牛乳的胶束形式的酪蛋白。用于对比的组合物均不含胶束酪蛋白，对于可比较的增重来说，该组合物产生更多的龋齿。实施例2和3，对比实施例iv)和v)
制备了4种组合物，其中两种与本发明一致，而另两个用于对比。每个组合物均含30％蔗糖、32％面粉、5％酒酵母和2％微量养料(Gevral混合物)，剩下的30％由下列成分构成：实施例2：工业用脱脂牛奶粉实施例3：新鲜的工业用牛乳，于37-40℃脱脂并于70℃以下喷雾干
燥。对比实施例iv)：工业用脱脂鲜牛乳，加2N HCl酸化至pH5，用2N
KOH中和后喷雾干燥。对比实施例v)：基于大豆的脱脂牛奶粉的替代物，其组成与所述的
对比实施例iii)的相同。
使用六窝(每窝4只)刚出生的Osborne-Mendel大鼠。在它们出生后的第13天，将这些大鼠和它们的母亲转移至带栅格板的不锈钢笼中，按需饲喂Nafag提浓物颗粒和自来水直至它们出生后的第20天。然后将这些大鼠随机分成6组，每组大鼠用上述的四种组合物之一饲喂并按需给自来水。
在第21和22天，给每个大鼠100μl Streptococcus sobrinus(OMZ-176)和Actinomyces viscosus(Ny-1)的浓悬浮液，每天两次。
在第40天，测定大鼠的微生物学情况。为此，用口涂片(oralsmear)取样，将样品悬浮在5ml RTF液中，并将50μl试样点在两个固体培养基上，所述培养基为含5％血细胞溶解的人血的Columbia含血琼脂平板和含5％蔗糖的TYS琼脂平板。
含血琼脂平板用于测定可培养的厌氧细菌的总数，即厌氧菌落形成单位(cfu)的总数和A.viscosus的菌落形成单位数。TS琼脂平板用于测定在这些平板上可培养的细菌的总数和S.sobrinus的菌落形成单位数。结果见下表II。表II实施例序号 含血琼脂 TYS琼脂
cfu×106 cfu×106
总计 A.viscosus 总计 S.sobrinus2 78.0 45.8％ 63.4 15.0％3 55.0 39.6％ 40.8 11.1％iv) 62.0 18.5％ 44.6 45.9％v) 51.0 8.3％ 41.0 54.8％
给予大鼠的实施例2和3的组合物含有得自牛乳的胶束形式的酪蛋白，由表II可以看出它们对口腔菌丛具有益的作用，这是因为它们显著降低了S.sobrinus的比例而同时增加其中的A.viscosus的比例。由给对比实施例iv)和v)组合物作食物的大鼠中却观察到相反的现象；所述的组合物中，第一种含胶束结构已被破坏的酪蛋白，而第二种根本不含任何酪蛋白。
在第40天，用常规方法测定牙斑情况、龋(初裂、深裂和光滑表面的龋)数及增重。结果用与表I类似的方法表示，见下表III。实施例序号 牙斑 初裂 深裂 光滑表面龋 增重2 1.6 9.3 4.5 4.8 1163 1.3 8.8 3.9 4.3 111iv) 1.6 9.9 5.8 7.3 112v) 1.7 10.9 8.4 9.6 106
由表III结果可以看出，给予大鼠作食物的实施例3组合物与给予大鼠作食物的实施例2组合物一样好，甚至比它还好；所述实施例3组合物含得自鲜牛乳的胶束结构未被破坏的酪蛋白，所述实施例2组合物也含得自牛乳的胶束形式的酪蛋白。相反，对比实施例iv)组合物和对比实施例v)组合物对于可比较的增重来说，却产生更多的龋齿；所述对比实施例iv)组合物含得自鲜牛乳的胶束结构已被破坏的酪蛋白，所述对比实施例v)组合物根本不含任何酪蛋白。实施例4和5，对比实施例vi)
制备了3种组合物，其中两种与本发明一致，而第三个用于对比。每个组合物均含40％蔗糖、22％面粉、5％酒酵母和2％微量养料(Gevral混合物)，剩下的30％由下列成分构成：实施例4：工业用脱脂牛奶粉实施例5：脱脂牛奶粉的替代物，它包含：
胶束酪蛋白 44.4％
乳糖 49.2％
Na3(C6H5O7)2.2H2O 2.4％
KCl 1.1％
MgCl2·6H2O 0.6％
K2HPO4.3H2O 2.3％
(该胶束酪蛋白经新鲜工业用脱脂牛乳的微量过滤和喷雾干燥制得)对比实施例vi)：基于酪蛋白酸盐的脱脂牛奶粉的替代物，其组成与
所述的对比实施例i)的相同。
使用六窝(每窝4只)刚出生的Osborne-Mendel大鼠。在它们出生后的第13天，将这些大鼠和它们的母亲转移至带栅格板的不锈钢笼中，按需饲喂Nafag提浓物颗粒和自来水直至它们出生后的第20天。然后将这些大鼠随机分成6组，每组大鼠用上述的三种组合物之一饲喂并按需给自来水。
在第21和22天，给每个大鼠100μl Streptococcus sobrinus(OMZ-176)和Actinomyces viscosus(Ny-1)的浓悬浮液，每天两次。
在第40天，测定大鼠的微生物学情况。为此，通过磨每组6只大鼠的左上颌的第一和第二磨牙来制备牙斑样品，方法与上述实施例2、3、iv)和v)的相同，结果见下表IV。
表IV实施例序号 含血琼脂 TYS 琼脂
cfu×106 cfu×106
总计 A.viscosus 总计 S.sobrinus
4 4.3 31.8％ 5.1 59.4％
5 3.9 29.2％ 5.3 60.1％
vi) 5.7 12.0％ 4.9 84.1％
由表IV可以看出，表IV中的结果进一步证实了表II中的结果，给予大鼠的实施例4和5组合物对口腔菌丛具有益的作用，这是因为它们显著降低了S.sobrinus的比例而同时增加其中的A.viscosus的比例；所述组合物含得自牛乳的胶束形式的酪蛋白。由给对比实施例vi)组合物作食物的大鼠中却观察到相反的现象；所述的组合物含胶束结构已被破坏的酪蛋白。
在第40天，用常规方法测定牙斑情况、龋(初裂、深裂和光滑表面的龋)数及增重。结果用与表I类似的方法表示，见下表V。
表V实施例序号 牙斑 初裂 深裂 光滑表面龋 增重
4 1.8 11.0 8.0 13.9 100
5 1.2 10.1 4.4 9.6 99
vi) 2.0 11.4 9.5 19.3 100
由表V结果可以看出，实施例5组合物若与对比实施例vi)组合物的作用相比较，表现出特别明显的防龋特性；所述实施例5组合物含得自用微量过滤法保留了胶束形式的鲜牛乳的酪蛋白，所述对比实施例vi)组合物含胶束形式已被破坏的牛乳酪蛋白。考虑到这组实施例组合物中的蔗糖含量为40％，而上一组实施例组合物的却为30％，因此，实施例4的结果也比实施例vi)的要好。实施例6
用于婴儿的食用组合物通过将下列成分按下述比例混合来制备：
胶束酪蛋白 6.3％
脱矿物质的乳清粉 68.6％
(12.5％蛋白)
乳脂 20.6％
玉米油 4.4％
K2HPO4 0.1％(该胶束酪蛋白通过将鲜工业用脱脂牛乳微量过滤并喷雾干燥制得)实施例7
用于婴儿的食用组合物通过将下列成分按下述比例混合来制备：
胶束酪蛋白 12.6％
脱矿物质的乳清粉 22.9％
(12.5％蛋白)
乳糖 34.9％
乳脂 20.4％
玉米油 4.6％
矿物质、微量养料及水 4.6％
(该胶束酪蛋白通过将新鲜的工业用脱脂牛乳微量过滤并喷雾干燥制得)。实施例8
基于牛乳的饮料组合物按下述方法制备：将3-15g由鲜的工业用脱脂牛乳的微量过滤后喷雾干燥所制得的胶束酪蛋白与5-20g乳糖用70-90g水混合，这些成分的总量应为100g。实施例9
粉末状咖啡稀奶油代用品组合物通过将下列成分按下述比例混合制得：
胶束酪蛋白 2-31％
乳糖 31-60％
部分氢化的椰子油 33％
矿物质、食用香料和水 5％
这些成分的总量应为100％。
胶束酪蛋白通过新鲜工业用脱脂牛乳的微量过滤和喷雾干燥来制备。
Claims (6)
1.脱脂乳的胶束酪蛋白在制备防龋组合物方面的用途。
2.按照权利要求1的用途，其中所述脱脂乳的胶束酪蛋白用于制备对口腔菌丛具有益的作用的组合物。
3.按照权利要求1的用途，其中所述胶束酪蛋白来自动物乳，尤其是来自牛乳、山羊乳或绵羊乳。
4.按照权利要求1的用途，其中所述胶束酪蛋白由动物乳的微量过滤获得。
5.按照权利要求1的用途，其中所述胶束酪蛋白由动物乳的超滤和/或透滤获得。
6.按照权利要求1-5中任一项的用途，其中，胶束酪蛋白和所述的不同于酪蛋白的乳成分被按与该乳中所遇到的相类似的比例混合。
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| CH3971/92A CH684773A5 (fr) | 1992-12-28 | 1992-12-28 | Composition alimentaire anti-cariogène. |
| CH3971/1992 | 1992-12-28 | ||
| CH3971/92 | 1992-12-28 |
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| PT686396E (pt) * | 1994-02-25 | 2001-01-31 | Nestle Sa | Composicao enterica que compreende caseina micelar nativa |
| ATE206877T1 (de) | 1995-06-16 | 2001-11-15 | Nestle Sa | Fluorierte kasein-mizellen |
| AUPO566297A0 (en) * | 1997-03-13 | 1997-04-10 | University Of Melbourne, The | Calcium phosphopeptide complexes |
| WO1998052524A1 (en) * | 1997-05-19 | 1998-11-26 | Colgate-Palmolive Company | Fluoride free dental remineralization |
| IE970541A1 (en) * | 1997-07-25 | 1999-01-27 | Michael Anthony Folan | Maternal immune secretions and their use in the treatment and/or prophylaxis of the buccal cavity |
| US5853704A (en) * | 1997-09-22 | 1998-12-29 | Colgate-Palmolive Company | Fluoride dentifrices of enhanced efficacy |
| AUPP494798A0 (en) * | 1998-07-29 | 1998-08-20 | Pacific Biolink Pty Limited | Protective protein formulation |
| BR9912780A (pt) * | 1998-08-07 | 2001-05-08 | Nestle Sa | Produto lácteo anticariogênico e sua utilização |
| EP1159951A1 (en) * | 2000-06-02 | 2001-12-05 | Societe Des Produits Nestle S.A. | Use of exogenous lactic bacteria strain against Actinomyces naeslundii-related diseases |
| CN1240372C (zh) | 1998-08-12 | 2006-02-08 | 雀巢制品公司 | 掺入口腔微生物区系的外源性乳酸菌 |
| AU2002345773A1 (en) * | 2001-06-08 | 2002-12-23 | Societe Des Produits Nestle S.A. | Sugar-based composition containing caseinoglycomacropeptide |
| GB0209384D0 (en) * | 2002-04-24 | 2002-06-05 | Pepsyn Ltd | Peptide composition |
| EP1608232B1 (en) * | 2003-03-18 | 2016-10-19 | Nestec S.A. | Method and compositions for improving animal health |
| US20050123490A1 (en) * | 2003-12-04 | 2005-06-09 | Kazue Yamagishi | Composition and method for prevention and treatment of dental caries |
| EP1625877A3 (en) | 2004-08-09 | 2006-03-08 | Friesland Brands B.V. | Functional milk fraction |
| EP2705826B1 (en) | 2006-02-09 | 2019-09-11 | The University of Melbourne | Fluoride composition and methods for dental mineralization |
| ES2348963T3 (es) * | 2006-03-27 | 2010-12-17 | Nestec S.A. | Utilizacion cosmetica de micelas de proteinas de suero. |
| GB0723523D0 (en) * | 2007-11-30 | 2008-01-09 | Glaxo Group Ltd | Novel use |
| US9055752B2 (en) * | 2008-11-06 | 2015-06-16 | Intercontinental Great Brands Llc | Shelf-stable concentrated dairy liquids and methods of forming thereof |
| UA112972C2 (uk) | 2010-09-08 | 2016-11-25 | Інтерконтінентал Грейт Брендс ЛЛС | Рідкий молочний концентрат з високим вмістом сухих речовин |
| EP3024428B1 (en) | 2013-07-23 | 2020-10-21 | The University of Melbourne | Compositions and methods for dental mineralization |
| ES2959412T3 (es) | 2013-12-24 | 2024-02-26 | Univ Melbourne | Composiciones estañosas estabilizadas |
| CA2990730A1 (en) * | 2015-06-29 | 2017-01-05 | Leprino Foods Company | Micellar casein for coffee creamers and other dairy products |
| EP4311576A3 (en) | 2017-03-14 | 2024-04-10 | The University of Melbourne | Treatment for gingivitis |
| NL2022324B1 (en) * | 2018-12-28 | 2020-07-23 | Ausnutria B V | Liquid composition comprised of a micellar casein concentrate |
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| EP0316938A2 (en) * | 1987-11-19 | 1989-05-24 | Danish Fancy Food Group A/S | Milk concentrate and process for the preparation thereof |
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| US4244971A (en) * | 1978-10-19 | 1981-01-13 | Kraft, Inc. | Process and products for the manufacture of cheese flavored products |
| NZ199891A (en) * | 1981-03-04 | 1985-07-31 | Univ Melbourne | Caries-inhibiting compositions containing casein or x-s-casein or phosuitin |
| DE3207505A1 (de) * | 1982-03-02 | 1983-09-08 | Rudolf 8034 Germering Schanze | Verfahren zur herstellung eines biologisch hochwertigen, naturbelassenen nahrungskonzentrats, das bei dem verfahren erhaltene produkt und seine verwendung |
| US5015628A (en) * | 1986-06-12 | 1991-05-14 | The University Of Melbourne | Anticariogenic phosphopeptides |
| CH671879A5 (zh) * | 1987-02-26 | 1989-10-13 | Nestle Sa | |
| JP2559867B2 (ja) * | 1988-06-03 | 1996-12-04 | ザ ヌトラスウィート カンパニー | 冷凍デザートおよびその製造法 |
| US5143741A (en) * | 1988-06-03 | 1992-09-01 | Podolski Joseph S | Concentrated, substantially non-aggregated casein micelles as a fat/cream substitute and a method for reducing the fat content in food products |
| FR2644983B1 (fr) * | 1989-03-30 | 1991-07-19 | Pierre Coquin | Produits alimentaires notamment boissons et desserts de longue conservation et leur obtention |
| FR2672494A1 (fr) * | 1991-02-13 | 1992-08-14 | Madinier Isabelle | Ferments prophylactiques favorisant la prevention de la carie dentaire. |
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| EP0316938A2 (en) * | 1987-11-19 | 1989-05-24 | Danish Fancy Food Group A/S | Milk concentrate and process for the preparation thereof |
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| ARCHIVE OF ORAL BIOLOGY VOL.26.NO.5 1981.1.1 E.REYNOLDSETAL Effect of milk on caries in cidence and bacterial compositi on of dental plaque in the * |
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| Publication number | Publication date |
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| AU5228193A (en) | 1994-07-07 |
| GR3031795T3 (en) | 2000-02-29 |
| CN1089779A (zh) | 1994-07-27 |
| NO934771L (no) | 1994-06-29 |
| CH684773A5 (fr) | 1994-12-30 |
| AU666786B2 (en) | 1996-02-22 |
| NO314432B1 (no) | 2003-03-24 |
| EP0604802A1 (fr) | 1994-07-06 |
| NO934771D0 (no) | 1993-12-22 |
| EP0604802B1 (fr) | 1999-08-18 |
| NZ250398A (en) | 1995-12-21 |
| DE69326054D1 (de) | 1999-09-23 |
| ES2136633T3 (es) | 1999-12-01 |
| DE69326054T2 (de) | 1999-12-09 |
| ZA939295B (en) | 1994-08-18 |
| ATE183368T1 (de) | 1999-09-15 |
| ZA939296B (en) | 1994-08-18 |
| FI935666A0 (fi) | 1993-12-16 |
| PH30890A (en) | 1997-12-23 |
| FI935666A (fi) | 1994-06-29 |
| DK0604802T3 (da) | 2000-03-13 |
| CA2111178A1 (en) | 1994-06-29 |
| CA2111178C (en) | 1999-05-25 |
| JP2887246B2 (ja) | 1999-04-26 |
| US5427769A (en) | 1995-06-27 |
| JPH06217701A (ja) | 1994-08-09 |
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