CN105324483B - 工程化苯丙氨酸解氨酶多肽 - Google Patents

工程化苯丙氨酸解氨酶多肽 Download PDF

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Publication number
CN105324483B
CN105324483B CN201480035201.3A CN201480035201A CN105324483B CN 105324483 B CN105324483 B CN 105324483B CN 201480035201 A CN201480035201 A CN 201480035201A CN 105324483 B CN105324483 B CN 105324483B
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polypeptide
pal
amino acid
seq
sequence
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Expired - Fee Related
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Chinese (zh)
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CN105324483A (zh
Inventor
吉伽特·W·哈思曼
尼古拉斯·J·阿加德
本杰明·米杰茨
乔纳森·弗罗姆
张希云
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Codexis Inc
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Codexis Inc
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y403/00—Carbon-nitrogen lyases (4.3)
    • C12Y403/01—Ammonia-lyases (4.3.1)
    • C12Y403/01005—Phenylalanine ammonia-lyase (4.3.1.5)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y403/00—Carbon-nitrogen lyases (4.3)
    • C12Y403/01—Ammonia-lyases (4.3.1)
    • C12Y403/01024—Phenylalanine ammonia-lyase (4.3.1.24)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Diabetes (AREA)
  • Hematology (AREA)
  • Neurology (AREA)
  • Neurosurgery (AREA)
  • Obesity (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Immunology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
CN201480035201.3A 2013-04-18 2014-04-17 工程化苯丙氨酸解氨酶多肽 Expired - Fee Related CN105324483B (zh)

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Applications Claiming Priority (5)

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US201361813586P 2013-04-18 2013-04-18
US61/813,586 2013-04-18
US201361897932P 2013-10-31 2013-10-31
US61/897,932 2013-10-31
PCT/US2014/034500 WO2014172541A2 (en) 2013-04-18 2014-04-17 Engineered phenylalanine ammonia lyase polypeptides

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AU (2) AU2014253900C1 (https=)
CA (1) CA2909719C (https=)
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HR (1) HRP20190928T1 (https=)
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MY (1) MY193198A (https=)
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NZ713396A (en) 2013-04-18 2020-06-26 Codexis Inc Engineered phenylalanine ammonia lyase polypeptides
WO2015161019A1 (en) 2014-04-16 2015-10-22 Codexis, Inc. Engineered tyrosine ammonia lyase
ES2881384T3 (es) 2015-05-13 2021-11-29 Synlogic Operating Co Inc Bacterias manipuladas para reducir la hiperfenilalaninemia
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CN106755160B (zh) * 2017-01-17 2019-08-02 湖州颐辉生物科技有限公司 一种组氨酸解氨酶及其用途
MX2019009615A (es) 2017-02-13 2019-10-14 Codexis Inc Polipeptidos dise?ados de fenilalanina amonio liasa.
CN110914288B (zh) * 2017-05-27 2023-05-05 宁波酶赛生物工程有限公司 工程化醛缩酶多肽及其应用
CN111655841A (zh) 2017-06-21 2020-09-11 同生运营公司 用于治疗紊乱的细菌
US10900055B2 (en) * 2018-06-12 2021-01-26 Codexis, Inc. Engineered tyrosine ammonia lyase
JP2021531749A (ja) * 2018-07-12 2021-11-25 コデクシス， インコーポレイテッド 操作されたフェニルアラニンアンモニアリアーゼポリペプチド
AU2019373208A1 (en) * 2018-10-29 2021-05-13 Codexis, Inc. Engineered DNA polymerase variants
CA3122524A1 (en) 2018-12-14 2020-06-18 Codexis, Inc. Engineered tyrosine ammonia lyase
WO2021168060A1 (en) * 2020-02-19 2021-08-26 Trustees Of Tufts College Methods for engineering amino acid ammonia lyase enzymes and enzymes thereby obtained
BR112022018571A2 (pt) * 2020-03-20 2022-11-01 Synlogic Operating Co Inc Microrganismos manipulados para reduzir a hiperfenilalaninemia
CN116847860A (zh) * 2020-12-31 2023-10-03 同生运营公司 经工程化以减轻高苯丙氨酸血症的微生物
CA3220735A1 (en) * 2021-05-21 2022-11-24 Codexis, Inc. Engineered methionine gamma lyase variants
CN114015636B (zh) * 2021-12-08 2023-09-29 天地壹号饮料股份有限公司 重组醋酸菌及其制备方法和应用
CN114457151B (zh) * 2022-01-27 2024-06-11 宁夏医科大学总医院 一种用于检测苯丙氨酸羟化酶基因突变的检测试剂盒及其检测方法
CN117417925A (zh) * 2022-07-18 2024-01-19 浙江泽科塔生物医药有限公司 Pal变体、包含该pal变体的药物组合物以及用于制备该pal变体的方法
CN115518691B (zh) * 2022-10-14 2023-11-21 天津大学 一种具有类漆酶活性的人工酶及制备方法和应用
CN115873837A (zh) * 2022-11-18 2023-03-31 安徽盈科生物科技有限公司 高表达的新型的苯丙氨酸解氨酶
CN116478975B (zh) * 2023-06-16 2023-09-01 苏州优信合生技术有限公司 高活性苯丙氨酸解氨酶突变体及其表达菌株
TW202519654A (zh) * 2023-09-19 2025-05-16 日商積水化學工業股份有限公司 解氨酶及其用途

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US20180016568A1 (en) 2018-01-18
US10494623B2 (en) 2019-12-03
LT2986722T (lt) 2019-09-10
US10487319B2 (en) 2019-11-26
CN105324483A (zh) 2016-02-10
US20180037880A1 (en) 2018-02-08
ES2890955T3 (es) 2022-01-25
JP2018166524A (ja) 2018-11-01
DK3521423T3 (da) 2021-09-06
NZ713396A (en) 2020-06-26
AU2014253900B2 (en) 2019-09-19
US10941391B2 (en) 2021-03-09
JP6637410B2 (ja) 2020-01-29
US20200199566A1 (en) 2020-06-25
JP2022183356A (ja) 2022-12-08
PH12015502412B1 (en) 2016-02-22
AU2014253900A1 (en) 2015-11-12
PL2986722T3 (pl) 2019-10-31
US20210222146A1 (en) 2021-07-22
PE20151775A1 (es) 2015-12-02
AU2014253900C1 (en) 2020-04-02
US20200032233A1 (en) 2020-01-30
US20200032234A1 (en) 2020-01-30
US10640759B2 (en) 2020-05-05
US20190055535A1 (en) 2019-02-21
US20200032235A1 (en) 2020-01-30
US10144924B2 (en) 2018-12-04
US9611468B2 (en) 2017-04-04
US20200032236A1 (en) 2020-01-30
SMT201900315T1 (it) 2019-07-11
CN109486803B (zh) 2022-10-25
PH12015502412A1 (en) 2016-02-22
US20140314843A1 (en) 2014-10-23
US20200399627A1 (en) 2020-12-24
US10160963B2 (en) 2018-12-25
ES2729048T3 (es) 2019-10-30
SI2986722T1 (sl) 2019-07-31
SG11201508598YA (en) 2015-11-27
KR102218930B1 (ko) 2021-02-23
US10717974B2 (en) 2020-07-21
US20170191050A1 (en) 2017-07-06
RS58802B1 (sr) 2019-07-31
MX2015014662A (es) 2016-03-03
US11136571B2 (en) 2021-10-05
US20200231959A1 (en) 2020-07-23
EP3971288A1 (en) 2022-03-23
EP2986722A4 (en) 2017-02-22
AU2019272057A1 (en) 2019-12-19
US20200040325A1 (en) 2020-02-06
WO2014172541A2 (en) 2014-10-23
BR112015026160A2 (pt) 2017-10-10
EA034034B1 (ru) 2019-12-20
US11913045B2 (en) 2024-02-27
PT2986722T (pt) 2019-06-21
EP3521423A1 (en) 2019-08-07
KR20150143816A (ko) 2015-12-23
JP7129715B2 (ja) 2022-09-02
HRP20190928T1 (hr) 2019-07-26
HUE055899T2 (hu) 2022-01-28
US11401515B2 (en) 2022-08-02
US20190241882A1 (en) 2019-08-08
AU2019272057B2 (en) 2021-05-27
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