CN105296397A - Method for culturing aquaculture rhodospirillum - Google Patents
Method for culturing aquaculture rhodospirillum Download PDFInfo
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- CN105296397A CN105296397A CN201510766558.5A CN201510766558A CN105296397A CN 105296397 A CN105296397 A CN 105296397A CN 201510766558 A CN201510766558 A CN 201510766558A CN 105296397 A CN105296397 A CN 105296397A
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- rhodospirillum
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Abstract
The invention discloses a method for culturing aquaculture rhodospirillum. The method comprises the following steps: performing salt-tolerance seed selection of rhodospirillum and sulfur-free rhodospirillum, obtaining salt-tolerance mixed rhodospirillum strains by employing a gradient cultivation method, and obtaining the salt-tolerance mixed rhodospirillum through culturing. The method has the beneficial effect that the salt-tolerance mixed rhodospirillum has no toxicity on aquatic animals, has no toxicity and side effect on human body, and can effectively improve the water area condition of an aquatic product culture water area, application method is simple, and application prospect is good; by adding the salt-tolerance mixed rhodospirillum in the aquatic product growth water area, content of an organic pollutant in culture water area sediment is effectively reduced, so that the culture water area environment is in favor of growth of aquatic products such as fish, shrimps and crabs.
Description
Technical field
The present invention relates to technical field of aquaculture, more specifically to a kind of cultural method of aquaculture rhodospirillum.
Background technology
Aquaculture is the activity in production of breeding, cultivating and gather in the crops aquatic animals and plants under manual control, generally comprises the whole process forming fishery products under artificial breeding management from seed, broadly also can comprise Enhancement of fishery resources.Aquaculture has the modes such as extensive cultivation, intensive culture and high density culture, and extensive cultivation is in medium and small natural water area, throw in seed, forms fishery products completely by natural bait, as lake and reservoir breed fish and shallow sea support shellfish etc.Intensive culture forms fishery products with bait throwing in, fertilizing method in comparatively small size pond, as aqiuculrue, cage culture and enclosure culture, net enclosure culture etc., high density culture adopts flowing water, temperature control, oxygenation and the methods such as high-quality bait of throwing something and feeding, high-density breeding is carried out in small size pond, thus acquisition high yield, as Running Water Intensive Fish Farming, shrimp etc.; Rhodospirillum is lived in the mud in lake, pond, is a kind of typical amphitrophy type bacterium.When rhodospirillum grows under difficult environmental conditions, its nutrient type can change, and do not having under organic condition, it can utilize luminous energy, and stabilizing carbon dioxide fixes organism; Having under organic condition, it can utilize again organism to grow; According to its feature, start at present to use rhodospirillum to purify the organic waste water of high density in efforts at environmental protection, to reach protection of the environment, the object of decontamination.
Summary of the invention
The object of the present invention is to provide one all can have no side effect to animal, human body, improve condition of water quality, make the cultural method of the growth aquaculture rhodospirillum being more suitable for fish, shrimp, crab etc. fishery products.
The cultural method of a kind of aquaculture rhodospirillum of technical solution of the present invention is achieved by following technical proposals;
A cultural method for aquaculture rhodospirillum, carries out according to following step:
Step 1: rhodospirillum and sulfur-free rhodospirillum are inoculated in substratum and cultivate, consisting of of described substratum: through the seawater 1L of sterilizing, ammonium chloride 0.5-1.4g, SODIUM PHOSPHATE, MONOBASIC 0.8-2.5g, yeast extract 3-8g, sodium-acetate 4-6g, ammonium sulfate 0.5-1.3g, Sodium orthomolybdate 0.06-0.13g and boric acid 0.06-0.13g, PH are 7.0-8.0, cultivate 4-9 days;
Step 2: cultivated cultivating the hybrid bacterial strain obtained in described step 1 by gradient method of cultivation, after continuous passage 4-6 generation, obtaining can in the salt tolerant mixture of red spirillum strain of stable growth under sea conditions;
Step 3: by the salt tolerant mixture of red spirillum strain obtained in described step 2, at 24-34 DEG C, intensity of illumination is continue under the condition of 20000-40000LX to cultivate, consisting of of selected substratum: aquaculture waters bed mud 0.5L, ammonium chloride 0.5-1.8g, magnesium sulfate 1.8-3.2g, L-glutamic acid 0.2-0.43g, ferrous sulfate 0.3-0.73g and aquaculture seawater 0.5L, PH are 7.0-8.0, cultivate 9-13 days, obtain salt tolerant mixture of red spirillum.
Preferably, in described step 1, consisting of of described substratum: through the seawater 1L of sterilizing, ammonium chloride 1.0g, SODIUM PHOSPHATE, MONOBASIC 2.0g, yeast extract 5.0g, sodium-acetate 5.3g, ammonium sulfate 0.9g, Sodium orthomolybdate 0.1g and boric acid 0.1g, PH are 7.5, and described incubation time is 6 days.
Preferably, in described step 2, described continuous passage was 5 generations.
Preferably, in described step 3, described culture condition is: culture temperature is 29 DEG C, and intensity of illumination is 30000LX, and described substratum consists of: aquaculture waters bed mud 0.5L, ammonium chloride 1.1g, magnesium sulfate 2.5g, L-glutamic acid 0.35g, ferrous sulfate 0.6g and aquaculture seawater 0.5L, PH is 7.5, described incubation time 10 days.
The technology of the present invention beneficial effect:
The cultural method of a kind of aquaculture rhodospirillum of technical solution of the present invention, this salt tolerant mixture of red spirillum, to hydrocoles nontoxicity, to human non-toxic's side effect, and effectively improves the water area condition in aquaculture waters, application method is simple, has a good application prospect; The salt tolerant mixture of red spirillum that the inventive method obtains is added with in fishery products growth waters, effectively reduce the content of this Cultivated water Organic Pollutants in Sedimentary Mud, make the growth of the fish of environmental benefits in aquaculture, shrimp, crab etc. the fishery products of this Cultivated water.
All can have no side effect to animal, human body, improve condition of water quality, make the growth being more suitable for fish, shrimp, crab etc. fishery products
Embodiment
Understand technical solution of the present invention for ease of those skilled in the art, now in conjunction with specification sheets embodiment, technical solution of the present invention is described further.
Embodiment 1
A cultural method for aquaculture rhodospirillum, carries out according to following step:
Step 1: rhodospirillum and sulfur-free rhodospirillum are inoculated in substratum and cultivate, consisting of of substratum: through the seawater 1L of sterilizing, ammonium chloride 0.5g, SODIUM PHOSPHATE, MONOBASIC 0.8g, yeast extract 3g, sodium-acetate 4g, ammonium sulfate 0.5g, Sodium orthomolybdate 0.06g and boric acid 0.06g, PH are 8, cultivate 9 days;
Step 2: cultivated cultivating the hybrid bacterial strain obtained in step 1 by gradient method of cultivation, after continuous passage 6 generation, obtaining can in the salt tolerant mixture of red spirillum strain of stable growth under sea conditions;
Step 3: by the salt tolerant mixture of red spirillum strain obtained in step 2, at 34 DEG C, intensity of illumination is continue under the condition of 40000LX to cultivate, consisting of of selected substratum: aquaculture waters bed mud 0.5L, ammonium chloride 0.5g, magnesium sulfate 1.8g, L-glutamic acid 0.2g, ferrous sulfate 0.3g and aquaculture seawater 0.5L, PH are 8.0, cultivate 13 days, obtain salt tolerant mixture of red spirillum.
Embodiment 2
A cultural method for aquaculture rhodospirillum, carries out according to following step:
Step 1, rhodospirillum and sulfur-free rhodospirillum are inoculated in substratum and cultivate, consisting of of substratum: through the seawater 1L of sterilizing, ammonium chloride 1.4g, SODIUM PHOSPHATE, MONOBASIC 2.5g, yeast extract 8.0g, sodium-acetate 6.0g, ammonium sulfate 1.3g, Sodium orthomolybdate 0.13g and boric acid 0.13g, PH is 7, cultivates 4 days;
Step 2, is cultivated cultivating the hybrid bacterial strain obtained in step 1 by gradient method of cultivation, and after continuous passage 4 generation, obtaining can in the salt tolerant mixture of red spirillum strain of stable growth under sea conditions;
Step 3, by the salt tolerant mixture of red spirillum strain obtained in step 2, at 24 DEG C, intensity of illumination is continue under the condition of 20000LX to cultivate, consisting of of selected substratum: aquaculture waters bed mud 0.5L, ammonium chloride 1.8g, magnesium sulfate 3.2g, L-glutamic acid 0.43g, ferrous sulfate 0.73g and aquaculture seawater 0.5L, PH is 7.0, cultivates 9 days, obtains salt tolerant mixture of red spirillum.
Embodiment 3
A cultural method for aquaculture rhodospirillum, carries out according to following step:
Step 1, rhodospirillum and sulfur-free rhodospirillum are inoculated in substratum and cultivate, consisting of of substratum: through the seawater 1L of sterilizing, ammonium chloride 1.0g, SODIUM PHOSPHATE, MONOBASIC 2.0g, yeast extract 5.0g, sodium-acetate 5.3g, ammonium sulfate 0.9g, Sodium orthomolybdate 0.1g and boric acid 0.1g, PH is 7.5, cultivates 6 days;
Step 2, is cultivated cultivating the hybrid bacterial strain obtained in step 1 by gradient method of cultivation, and after continuous passage 5 generation, obtaining can in the salt tolerant mixture of red spirillum strain of stable growth under sea conditions;
Step 3, by the salt tolerant mixture of red spirillum strain obtained in step 2, at 30 DEG C, intensity of illumination is continue under the condition of 30000LX to cultivate, consisting of of selected substratum: aquaculture waters bed mud 0.5L, ammonium chloride 1.1g, magnesium sulfate 2.5g, L-glutamic acid 0.35g, ferrous sulfate 0.6g and aquaculture seawater 0.5L, PH is 7.5, cultivates 10 days, obtains salt tolerant mixture of red spirillum.
This salt tolerant mixture of red spirillum, to hydrocoles nontoxicity, to human non-toxic's side effect, and effectively improves the water area condition in aquaculture waters, and application method is simple, has a good application prospect; The salt tolerant mixture of red spirillum that the inventive method obtains is added with in fishery products growth waters, effectively reduce the content of this Cultivated water Organic Pollutants in Sedimentary Mud, make the growth of the fish of environmental benefits in aquaculture, shrimp, crab etc. the fishery products of this Cultivated water.
Technical solution of the present invention is exemplarily described invention above in conjunction with the embodiments; obvious specific implementation of the present invention is not subject to the restrictions described above; as long as have employed the various unsubstantialities that method of the present invention is conceived and technical scheme is carried out to improve; or the design of invention and technical scheme directly applied to other occasion, all within protection scope of the present invention without to improve.
Claims (4)
1. a cultural method for aquaculture rhodospirillum, is characterized in that: carry out according to following step:
Step 1: rhodospirillum and sulfur-free rhodospirillum are inoculated in substratum and cultivate, consisting of of described substratum: through the seawater 1L of sterilizing, ammonium chloride 0.5-1.4g, SODIUM PHOSPHATE, MONOBASIC 0.8-2.5g, yeast extract 3-8g, sodium-acetate 4-6g, ammonium sulfate 0.5-1.3g, Sodium orthomolybdate 0.06-0.13g and boric acid 0.06-0.13g, PH are 7.0-8.0, cultivate 4-9 days;
Step 2: cultivated cultivating the hybrid bacterial strain obtained in described step 1 by gradient method of cultivation, after continuous passage 4-6 generation, obtaining can in the salt tolerant mixture of red spirillum strain of stable growth under sea conditions;
Step 3: by the salt tolerant mixture of red spirillum strain obtained in described step 2, at 24-34 DEG C, intensity of illumination is continue under the condition of 20000-40000LX to cultivate, consisting of of selected substratum: aquaculture waters bed mud 0.5L, ammonium chloride 0.5-1.8g, magnesium sulfate 1.8-3.2g, L-glutamic acid 0.2-0.43g, ferrous sulfate 0.3-0.73g and aquaculture seawater 0.5L, PH are 7.0-8.0, cultivate 9-13 days, obtain salt tolerant mixture of red spirillum.
2. the cultural method of a kind of aquaculture rhodospirillum according to claim 1, it is characterized in that: in described step 1, consisting of of described substratum: through the seawater 1L of sterilizing, ammonium chloride 1.0g, SODIUM PHOSPHATE, MONOBASIC 2.0g, yeast extract 5.0g, sodium-acetate 5.3g, ammonium sulfate 0.9g, Sodium orthomolybdate 0.1g and boric acid 0.1g, PH is 7.5, and described incubation time is 6 days.
3. the cultural method of a kind of aquaculture rhodospirillum according to claim 1, is characterized in that: in described step 2, and described continuous passage was 5 generations.
4. the cultural method of a kind of aquaculture rhodospirillum according to claim 1, it is characterized in that: in described step 3, described culture condition is: culture temperature is 29 DEG C, and intensity of illumination is 30000LX, and described substratum consists of: aquaculture waters bed mud 0.5L, ammonium chloride 1.1g, magnesium sulfate 2.5g, L-glutamic acid 0.35g, ferrous sulfate 0.6g and aquaculture seawater 0.5L, PH is 7.5, incubation time 10 days.
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| CN104498409A (en) * | 2014-12-29 | 2015-04-08 | 天津振邦水产养殖有限公司 | Method for culturing rhodospirillum for degrading organic contaminations of aquiculture |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104498409A (en) * | 2014-12-29 | 2015-04-08 | 天津振邦水产养殖有限公司 | Method for culturing rhodospirillum for degrading organic contaminations of aquiculture |
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Application publication date: 20160203 |