CN105254407A - Black fungus cultivation material and method - Google Patents

Black fungus cultivation material and method Download PDF

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CN105254407A
CN105254407A CN201510638782.6A CN201510638782A CN105254407A CN 105254407 A CN105254407 A CN 105254407A CN 201510638782 A CN201510638782 A CN 201510638782A CN 105254407 A CN105254407 A CN 105254407A
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black fungus
black
wood chip
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cultivation material
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CN105254407B (en
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刘振东
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AGRICULTURAL AND ANIMAL HUSBANDRY COLLEGE OF TIBET UNIVERSITY
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Abstract

The invention provides a black fungus cultivation material and method, and relates to a fungus cultivation material and method. The black fungus cultivation material and method solve the problems that when black fungi are produced through a traditional bagged material field cultivation mode in the Linzhi area, black fungi are prone to being infected by sundry fungi, the fungus period is short, yield is low and a large amount of timber needs to be consumed. The black fungus cultivation material is formed by mixing dry materials with water. The dry materials are prepared from cyclobalanopsis wood bits, humus, pine tree wood bits, corn straw smashed objects, wheat bran, gypsum and lime. The cultivation method includes the steps of firstly, preparing the cultivation material; secondly, inoculating black fungus bacteria; thirdly, conducting three-dimensional greenhouse cultivation. The three-dimensional greenhouse cultivation technology is adopted, lands are effectively solved, water sources are saved, and the labor cost is reduced; in addition, the risk that black fungi are infected by sundry bacteria is lowered, and yield and quality of black fungi are improved. Wood bit waste is adopted for the black fungus cultivation material, and therefore the felling amount of trees is greatly decreased, the addition amount of broad-leaf wood bits is greatly reduced by modifying the formula, and protection of deep forest resources is facilitated.

Description

A kind of black fungus cultivation material and black fungus cultural method
Technical field
The present invention relates to a kind of auricularia auriculajudae culture material and auricularia auriculajudae cultural method.
Background technology
Fromlingzhi, tibet Gu claims work cloth, is positioned at Tibet Autonomous Region southeast, In The Middle And Lower Reaches, the Yarlung Zangbo River, is positioned at north latitude 26 ° 52 ' ~ 30 ° 40 ', between east longitude 92 ° 09 ' ~ 98 ° 47 '.Linzhi's mean sea level 3100 meters, total area 116175Km 2, wherein forest land area 2,640,000 hectares, forest coverage 46.09%, be the third-largest forest zone of China, 80% of Tibet forest all concentrates on here.The virgin forest of Linzhi is intact, the ancient cypress in Tibet, Himalaya fir, tree fern, and common pine tree, birch, Qinggang oak ..., have the title of " the natural museum of natural history "; In addition Linzhi is rainy by the impact of Bay of Bengal warm moist air for summer, is also referred to as the south of the River in Tibet.
Containing 10.6 grams, protein, 0.2 gram, fat, 65 grams, carbohydrate, robust fibre 7 grams, calcium 375 milligrams, 201 milligrams, phosphorus, iron 185 milligrams in the every 100 grams of dry products of black fungus, in addition containing vitamins B 10.15 milligram, vitamins B 20.55 milligram, 2.7 milligrams, nicotinic acid, i.e. edible but also pharmaceutically acceptable are a kind of nutritious edible mushroomss
Rainy and the day and night temperature of Linzhi is comparatively large, therefore with traditional Bag Material the mode of planting produce black fungus, often therefore face easy infection miscellaneous bacteria, the ear phase is short, and the series of problems such as yield poorly.And to pendulum bacterium plot, traditional Bag Material cultivation of auricularia auricula mode, requires that higher and floor space is comparatively large, every square metre of bacterium bag only putting 60 ~ 70 16cm × 34cm.Because the down payment Ba Yi district in Linzhi city and each main county and town are all in area, river valley, can tilling the land, there is a serious shortage in the supply for area, and along with developing of black fungus industry, black fungus is produced the situation of striving ground with grain and highlighted gradually.
Virgin forest; the local ecosystem is very fragile and responsive, seems even more important to other areas of protection ratio of forest, and a large amount of broad-leaved wood chip of traditional black fungus need of production is as base-material; a large amount of timber need be consumed, this be contrary to the protection of forest ecology.
Consider the protection to virgin forest, and the ecosystem that locality is very fragile and responsive, need a large amount of broad-leaved wood chip as base-material, need consume a large amount of timber traditional black fungus mode of production Linzhi and be not suitable for.
Summary of the invention
The present invention be in order to solving the traditional Bag Material in the Nyingchi Prefecture mode of planting produce black fungus easy infection miscellaneous bacteria, ear phase short, yield poorly, and the problem of a large amount of timber need be consumed, and a kind of black fungus cultivation material provided and black fungus cultural method.
Black fungus cultivation material of the present invention is mixed by siccative and water; Siccative is minced by Qinggang wood chip of 20% ~ 45%, soil ulmin, the pine tree wood chip of 10% ~ 20%, the corn stalk powder of 10% ~ 20% of 10% ~ 25% by weight percentage, the wheat bran of 8% ~ 12%, the gypsum of 0.5% ~ 1% and 1% ~ 2% lime make; Wherein, soil ulmin and pine tree wood chip must use after mixed fermentation; The mass ratio of water and siccative is 100:65 ~ 67.
Black fungus of the present invention is cultivated according to the following steps:
One, prepare culture material: siccative takes Qinggang wood chip of 20% ~ 45% by weight percentage, soil ulmin, the pine tree wood chip of 10% ~ 20%, the corn stalk powder of 10% ~ 20% of 10% ~ 25% minces, the wheat bran of 8% ~ 12%, the gypsum of 0.5% ~ 1% and 1% ~ 2% lime; Then soil ulmin is mixed in the environment being positioned over 30 ~ 40 DEG C with pine tree wood chip the 12 ~ 18d that ferments, then Qinggang wood chip taken, corn stalk powder are minced, wheat bran, gypsum and lime adds and mixes; Again the water taken is added afterwards and stir, packing; Wherein, the mass ratio of water and siccative is 100:65 ~ 67;
Two, the culture material autoclaving 2 ± 0.1h prepared by step one, then aseptic technique Jie Zhong ?edible fungus, the culturing room being placed in 25 ~ 28 DEG C is cultured to mycelia and grows to bottom bacterium bag, then is put into after-ripening mycelia in 15 ~ 20 DEG C of environment;
Three, stereoscopic greenhouse cultivation, two ends, greenhouse are provided with the air door of high 3.5 ~ 4m, wide 2 ~ 3m, are provided with the support of spraying facility and outstanding flagging mushroom bag in greenhouse, and spraying nozzle exceeds more than rack beam 0.3m; Be suspended on support by bacterium bag by the mode connecing bacterium mouth down, often arrange the space leaving 2 ~ 4cm between upper and lower bacterium bag, often arrange bottom bacterium pocket position overhead height >=0.3m, the distance between two row bacterium bags is 5 ~ 8cm; It is 85% ~ 90% that temperature indoor relative humidity controls, temperature controls≤26 DEG C, and treat that ear bud grows to 1cm, early morning every day and dusk need each spray form water 1h, and earth's surface relative humidity is stabilized in 85% ~ 95%; After cultivating 30 ~ 40d, auricle launches, and reams, do not stay the basal part of the ear with blade along root, namely completes black fungus and cultivates.
The present invention adopts the effective Economization on land of stereoscopic greenhouse cultivation technique, saves water source, reduces cost of labor; And reduce the risk of auricularia auriculajudae bacteria infection, improve the seed output and quality of auricularia auriculajudae.
Black fungus cultivation material of the present invention adopts wood chip waste to decrease the cutting capacity of trees, and drastically reduce the area the addition of broad-leaved wood chip by the amendment of formula, is conducive to protecting dark woods resource.
Black fungus cultivation material of the present invention selects pine tree wood chip to be because pine tree growth cycle is shorter, and pine tree wood chip waste production is comparatively large, has the protection utilizing the forest reserves.Utilize saprophytic microorganisms a large amount of in soil ulmin under pine forests to decompose the material harmful to black fungus mycelial growth such as volatility grease, tannin etc. in pine tree wood chip in black fungus cultivation material of the present invention, and decomposed, ferment and change the nutritive substance of black fungus into.And soil ulmin itself, containing a large amount of mineral element and nitrogen, for the black fungus growth nitrogen provided in abundant nutritive substance, particularly soil ulmin is easy to absorption and the utilization of black fungus, can supply needed for black fungus primary stage of inoculation mycelia definite value in time.
Accompanying drawing explanation
Fig. 1 is that the back side 200xSEM of the black fungus " black 29 " that embodiment 1 is turned out schemes.
Fig. 2 is that the back side 200xSEM of the black fungus " black 29 " that embodiment 2 is turned out schemes.
Fig. 3 is that the outside of belly 200xSEM of the black fungus " black 29 " that embodiment 1 is turned out schemes.
Fig. 4 is that the outside of belly 200xSEM of the black fungus " black 29 " that embodiment 2 is turned out schemes.
Embodiment
Technical solution of the present invention is not limited to following cited embodiment, also comprises the arbitrary combination between each embodiment.
Embodiment one: present embodiment black fungus cultivation material is mixed by siccative and water; Siccative is minced by Qinggang wood chip of 20% ~ 45%, soil ulmin, the pine tree wood chip of 10% ~ 20%, the corn stalk powder of 10% ~ 20% of 10% ~ 25% by weight percentage, the wheat bran of 8% ~ 12%, the gypsum of 0.5% ~ 1% and 1% ~ 2% lime make; Wherein, soil ulmin and pine tree wood chip must use after mixed fermentation; The mass ratio of water and siccative is 100:65 ~ 67.
Present embodiment black fungus cultivation material selects pine tree wood chip to be because pine tree growth cycle is shorter, and pine tree wood chip waste production is comparatively large, has the protection utilizing the forest reserves.
The effect that gypsum can play solid and provide calcium ion is added in present embodiment black fungus cultivation material.Lime add the acid-basicity that can regulate black fungus cultivation material, and further provide calcium ion.
Embodiment two: the difference of present embodiment and embodiment one is: soil ulmin is soil ulmin under pine forests.Other is identical with embodiment one.
Embodiment three: the difference of present embodiment and embodiment one or two is: soil ulmin and pine tree wood chip mixed fermentation time is 12 ~ 18d, yeasting temperature is 30 ~ 40 DEG C.Other is identical with embodiment one or two.
Embodiment four: present embodiment black fungus is cultivated according to the following steps:
One, prepare culture material: siccative takes Qinggang wood chip of 20% ~ 45% by weight percentage, soil ulmin, the pine tree wood chip of 10% ~ 20%, the corn stalk powder of 10% ~ 20% of 10% ~ 25% minces, the wheat bran of 8% ~ 12%, the gypsum of 0.5% ~ 1% and 1% ~ 2% lime; Then soil ulmin is mixed in the environment being positioned over 30 ~ 40 DEG C with pine tree wood chip the 12 ~ 18d that ferments, then Qinggang wood chip taken, corn stalk powder are minced, wheat bran, gypsum and lime adds and mixes; Again the water taken is added afterwards and stir, packing; Wherein, the mass ratio of water and siccative is 100:65 ~ 67;
Two, the culture material autoclaving 2 ± 0.1h prepared by step one, then aseptic technique Jie Zhong ?edible fungus, the culturing room being placed in 25 ~ 28 DEG C is cultured to mycelia and grows to bottom bacterium bag, then is put into after-ripening mycelia in 15 ~ 20 DEG C of environment;
Three, stereoscopic greenhouse cultivation, two ends, greenhouse are provided with the air door of high 3.5 ~ 4m, wide 2 ~ 3m, are provided with the support of spraying facility and outstanding flagging mushroom bag in greenhouse, and spraying nozzle exceeds more than rack beam 0.3m; Be suspended on support by bacterium bag by the mode connecing bacterium mouth down, often arrange the space leaving 2 ~ 4cm between upper and lower bacterium bag, often arrange bottom bacterium pocket position overhead height >=0.3m, the distance between two row bacterium bags is 5 ~ 8cm; It is 85% ~ 90% that temperature indoor relative humidity controls, temperature controls≤26 DEG C, and treat that ear bud grows to 1cm, early morning every day and dusk need each spray form water 1h, and earth's surface relative humidity is stabilized in 85% ~ 95%; After cultivating 30 ~ 40d, auricle launches, and reams, do not stay the basal part of the ear with blade along root, namely completes black fungus and cultivates.
Without the need to adjust ph in present embodiment culture material layoutprocedure.
Embodiment five: the difference of present embodiment and embodiment four is: the culture material every bag siccative of packing in step one weighs 0.60 ± 0.1kg.Other step and parameter identical with embodiment four.
Embodiment six: the difference of present embodiment and embodiment four or five is: the pressure of step 2 mesohigh sterilizing is 0.1 ~ 0.15MPa.Other step and parameter identical with embodiment four or five.
Embodiment seven: the difference of one of present embodiment and embodiment four to six is: step 3 medium temperature chamber ground point selection is in wind sheltering, on the sunny side, irrigate and draining plot easily.Other step and parameter identical with one of embodiment four to six.
Embodiment eight: the difference of one of present embodiment and embodiment four to seven is: step 3 medium temperature chamber skeleton is full steel structure, arch shed type, the high 3.5 ~ 4m of canopy, wide 9 ~ 10m, long 25 ~ 30m.Other step and parameter identical with one of embodiment four to seven.
Embodiment nine: the difference of one of present embodiment and embodiment four to eight is: step 3 medium temperature chamber both sides are provided with overlay film and sunshade net.Other step and parameter identical with one of embodiment four to eight.
Overlay film and sunshade net liftable or roll in present embodiment.
Embodiment ten: the difference of one of present embodiment and embodiment four to nine is: in step 3 greenhouse, spraying nozzle is spaced apart 0.5m.Other step and parameter identical with one of embodiment four to nine.
Embodiment 11: the difference of one of present embodiment and embodiment four to ten is: step 3 often arranges continues to employ triangle circle between upper and lower bacterium bag and separate.Other step and parameter identical with one of embodiment four to ten.
Embodiment 12: the difference of one of present embodiment and embodiment four to ten one is: in step 3 greenhouse, temperature is higher, carries out ventilating and/or spray form water for cooling.Other step and parameter identical with one of embodiment four to ten one.
Embodiment 1
Black fungus adopts mode of planting traditionally to cultivate, and blackfungus culture medium matter is made up of Qinggang wood chip, the white birch wood chip of 30%, highland barley stalk, the highland barley bran of 8% and the gypsum of 2% of 15% of 45% by weight percentage.
Embodiment 2
Black fungus is cultivated by following steps of the present invention:
One, prepare culture material: siccative takes Qinggang wood chip of 40% by weight percentage, soil ulmin, the pine tree wood chip of 15%, the corn stalk powder of 10% of 25% minces, the wheat bran of 8%, the gypsum of 1% and 1% lime; Then soil ulmin is mixed in the environment being positioned over 30 ~ 40 DEG C with pine tree wood chip the 15d that ferments, then Qinggang wood chip taken, corn stalk powder are minced, wheat bran, gypsum and lime adds and mixes; Again the water taken is added afterwards and stir, packing; Wherein, the mass ratio of water and siccative is 100:66;
Two, the culture material 0.1 ~ 0.15MPa autoclaving 2h prepared by step one, then aseptic technique Jie Zhong ?edible fungus, the culturing room being placed in 25 ~ 28 DEG C is cultured to mycelia and grows to bottom bacterium bag, then is put into after-ripening mycelia in 15 ~ 20 DEG C of environment;
Three, stereoscopic greenhouse cultivation, two ends, greenhouse are provided with the air door of high 3.8m, wide 2.5m, are provided with the support of spraying facility and outstanding flagging mushroom bag in greenhouse, and spraying nozzle exceeds more than rack beam 0.3m; Be suspended on support by bacterium bag by the mode connecing bacterium mouth down, often arrange the space leaving 2 ~ 4cm between upper and lower bacterium bag, often arranging bottom bacterium pocket position is highly overhead 0.4m, and the distance between two row bacterium bags is 5 ~ 8cm; It is 85% ~ 90% that temperature indoor relative humidity controls, temperature controls≤26 DEG C, and treat that ear bud grows to 1cm, early morning every day and dusk need each spray form water 1h, and earth's surface relative humidity is stabilized in 85% ~ 95%; After cultivating 35d, auricle launches, and reams, do not stay the basal part of the ear with blade along root, namely completes black fungus and cultivates.
Embodiment 3
The present embodiment also adopts stereoscopic greenhouse to cultivate, be that Qinggang wood chip, the soil ulmin of 25%, the corn stalk powder of 20% that siccative in step one takes 45% by weight percentage mince with the difference of embodiment 2, the wheat bran of 8%, the gypsum of 1% and 1% lime.(not comprising pine tree wood chip)
Embodiment 4
The present embodiment also adopts stereoscopic greenhouse to cultivate, be that Qinggang wood chip, the pine tree wood chip of 20%, the corn stalk powder of 20% that siccative in step one takes 45% by weight percentage mince with the difference of embodiment 2, the wheat bran of 8%, the gypsum of 1% and 1% lime.(not comprising soil ulmin)
Experiment:
The method of embodiment 1 ~ 4 is selected to cultivate black fungus bacterial classification " black 29 ", " No. 2, ingot " and " No. 6, Tibet " respectively.Auricularia auriculajudae growing state is as shown in table 1.
Table 1
Black fungus output is as shown in table 2.
Table 2
Black fungus commodity performance is as shown in table 3.
Table 3
Bacterial classification Color and luster Ear shape Thickness
Embodiment 1 (plant) Black V29 Outside of belly brown back side tawny Bowl-shape Thicker
Embodiment 2 Black V29 Outside of belly black back side tawny Bowl-shape Thick
Embodiment 3 Black V29 Outside of belly black back side tawny Bowl-shape Thicker
Embodiment 4 Black V29 Outside of belly black back side tawny Bowl-shape Thicker
Embodiment 1 (plant) No. 2, ingot Outside of belly black back side tawny Chrysanthemum shape Thin
Embodiment 2 No. 2, ingot Outside of belly black back side tawny Chrysanthemum shape Thicker
Embodiment 3 No. 2, ingot Outside of belly black back side tawny Chrysanthemum shape Thin
Embodiment 4 No. 2, ingot Outside of belly black back side tawny Chrysanthemum shape Thin
Embodiment 1 (plant) No. 6, Tibet Outside of belly brown back side tawny Strip Thicker
Embodiment 2 No. 6, Tibet Outside of belly black back side tawny Strip Thick
Embodiment 3 No. 6, Tibet Outside of belly black back side tawny Strip Thicker
Embodiment 4 No. 6, Tibet Outside of belly black back side tawny Strip Thicker
Black fungus matter structure analysis such as table carries out the texture characteristic analyses such as black fungus Elastic hardness as indicated at 4 with black 29.
Table 4
Planting type Bacterial classification Elasticity Hardness Cohesive force Gumminess Chewiness Rebound resilience
Embodiment 1 (plant) Black V29 694.332 0.825 0.401 323.124 455.000 0.198
Embodiment 2 Black V29 800.544 0.850 0.445 664.412 941.520 0.254
Embodiment 3 Black V29 700.27 0.792 0.410 592.489 682.025 0.205
Embodiment 4 Black V29 750.652 0.841 0.432 607.258 847.21 0.244
Embodiment 1 (plant) No. 2, ingot 533.499 0.781 0.424 223.706 421.225 0.286
Embodiment 2 No. 2, ingot 792.835 0.821 0.439 721.231 1164.271 0.342
Embodiment 3 No. 2, ingot 675.365 0.742 0.410 458.114 300.752 0.227
Embodiment 4 No. 2, ingot 720.371 0.815 0.455 597.586 820.147 0.325
Embodiment 1 (plant) No. 6, Tibet 654.094 0.884 0.475 382.113 200.562 0.288
Embodiment 2 No. 6, Tibet 935.256 0.992 0.486 498.007 609.800 0.328
Embodiment 3 No. 6, Tibet 478.012 0.754 0.407 407.007 478.244 0.241
Embodiment 4 No. 6, Tibet 700.671 0.847 0.472 478.127 555.696 0.307
Trophic analysis:
The black fungus " black 29 " of turning out with embodiment 1 ~ 4 carries out trophic analysis, and trophic analysis result is as shown in table 5, mineral element and contents of heavy metal elements analytical results as shown in table 6.
Table 5
Table 6
The black fungus " black 29 " of turning out with scanning electron microscopic observation.Fig. 1 is that the back side 200xSEM of the black fungus " black 29 " that embodiment 1 is turned out schemes.Fig. 2 is that the back side 200xSEM of the black fungus " black 29 " that embodiment 2 is turned out schemes.Fig. 3 is that the outside of belly 200xSEM of the black fungus " black 29 " that embodiment 1 is turned out schemes.Fig. 4 is that the outside of belly 200xSEM of the black fungus " black 29 " that embodiment 2 is turned out schemes.
From scanning electron microscopic observation to, black fungus " black 29 " back side pubescence that embodiment 1 and embodiment 2 are turned out is all close and short and small; The equal unfairness of the outside of belly, but the black fungus that embodiment 2 is turned out " black 29 " outside of belly cell arrangement is more regular, and fold is more and close.

Claims (10)

1. a black fungus cultivation material, is characterized in that black fungus cultivation material is mixed by siccative and water; Siccative is minced by Qinggang wood chip of 20% ~ 45%, soil ulmin, the pine tree wood chip of 10% ~ 20%, the corn stalk powder of 10% ~ 20% of 10% ~ 25% by weight percentage, the wheat bran of 8% ~ 12%, the gypsum of 0.5% ~ 1% and 1% ~ 2% lime make; Wherein, soil ulmin and pine tree wood chip must use after mixed fermentation; The mass ratio of water and siccative is 100:65 ~ 67.
2. a kind of black fungus cultivation material according to claim 1, is characterized in that soil ulmin is soil ulmin under pine forests.
3. a kind of black fungus cultivation material according to claim 1, it is characterized in that soil ulmin and pine tree wood chip mixed fermentation time be 12 ~ 18d, yeasting temperature is 30 ~ 40 DEG C.
4. black fungus cultural method, is characterized in that black fungus is cultivated according to the following steps:
One, prepare culture material: siccative takes Qinggang wood chip of 20% ~ 45% by weight percentage, soil ulmin, the pine tree wood chip of 10% ~ 20%, the corn stalk powder of 10% ~ 20% of 10% ~ 25% minces, the wheat bran of 8% ~ 12%, the gypsum of 0.5% ~ 1% and 1% ~ 1.5% lime; Then soil ulmin is mixed in the environment being positioned over 30 ~ 40 DEG C with pine tree wood chip the 12 ~ 18d that ferments, then Qinggang wood chip taken, corn stalk powder are minced, wheat bran, gypsum and lime adds and mixes; Again the water taken is added afterwards and stir, packing; Wherein, the mass ratio of water and siccative is 100:65 ~ 67;
Two, the culture material autoclaving 2 ± 0.1h prepared by step one, then aseptic technique Jie Zhong ?edible fungus, the culturing room being placed in 25 ~ 28 DEG C is cultured to mycelia and grows to bottom bacterium bag, then is put into after-ripening mycelia in 15 ~ 20 DEG C of environment;
Three, stereoscopic greenhouse cultivation, two ends, greenhouse are provided with the air door of high 3.5 ~ 4m, wide 2 ~ 3m, are provided with the support of spraying facility and outstanding flagging mushroom bag in greenhouse, and spraying nozzle exceeds more than rack beam 0.3m; Be suspended on support by bacterium bag by the mode connecing bacterium mouth down, often arrange the space leaving 2 ~ 4cm between upper and lower bacterium bag, often arrange bottom bacterium pocket position overhead height >=0.3m, the distance between two row bacterium bags is 5 ~ 8cm; It is 85% ~ 90% that temperature indoor relative humidity controls, temperature controls≤26 DEG C, and treat that ear bud grows to 1cm, early morning every day and dusk need each spray form water 1h, and earth's surface relative humidity is stabilized in 85% ~ 95%; After cultivating 30 ~ 40d, auricle launches, and reams, do not stay the basal part of the ear with blade along root, namely completes black fungus and cultivates.
5. black fungus cultural method according to claim 4, is characterized in that the pressure of step 2 mesohigh sterilizing is 0.1 ~ 0.15MPa.
6. black fungus cultural method according to claim 4, is characterized in that step 3 medium temperature chamber ground point selection is in wind sheltering, on the sunny side, irrigate and draining plot easily.
7. black fungus cultural method according to claim 4, is characterized in that step 3 medium temperature chamber skeleton is full steel structure, arch shed type, the high 3.5 ~ 4m of canopy, wide 9 ~ 10m, long 25 ~ 30m.
8. black fungus cultural method according to claim 4, is characterized in that step 3 medium temperature chamber both sides are provided with overlay film and sunshade net.
9. black fungus cultural method according to claim 4, is characterized in that in step 3 greenhouse, spraying nozzle is spaced apart 0.5m.
10. black fungus cultural method according to claim 4, is characterized in that step 3 often arranges and continues to employ triangle circle between upper and lower bacterium bag and separate.
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CN108293602A (en) * 2017-08-16 2018-07-20 什邡昊阳农业发展有限公司 A kind of agaric compost and agaric cultural method
CN108551974A (en) * 2018-03-23 2018-09-21 晴隆县为民食用菌开发有限责任公司 A kind of wild cultivating method of black fungus

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