CN105613049B - A kind of cultural method of the black fungus strain of decomposable pine and cypress tree sawdust - Google Patents
A kind of cultural method of the black fungus strain of decomposable pine and cypress tree sawdust Download PDFInfo
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- CN105613049B CN105613049B CN201610134506.0A CN201610134506A CN105613049B CN 105613049 B CN105613049 B CN 105613049B CN 201610134506 A CN201610134506 A CN 201610134506A CN 105613049 B CN105613049 B CN 105613049B
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- pine
- black fungus
- culture medium
- cypress
- mycelia
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
Abstract
The present invention relates to a kind of cultural methods of the black fungus strain of decomposable pine and cypress tree sawdust, comprising: (1) is grown on pine tree black fungus and acquires and transfer and be separately cultured in culture medium；(2) it is forwarded to ordinary culture medium culture；(3) it is inoculated in pine tree sawdust medium bacterium bag and carries out Spawn incubation to covering with bacterium bag；(4) from being transferred in bacterium bag in common PDA culture medium.Present invention process is simple, at low cost, the black fungus strain of cultivation has the advantages that pine tree and cypress cultivation on sawdust black fungus can be made full use of, a large amount of pine and cypress sawdusts is avoided to waste compared with conventional black fungus strain, in China, cultivation of auricularia auricula kind is unique, has a good application prospect.
Description
Technical field
The invention belongs to black fungus strain field, in particular to a kind of cultivation of the black fungus strain of decomposable pine and cypress tree sawdust
Culture method.
Background technique
China is that edible fungus culturing big country, especially cultivation of auricularia auricula are extensive in Chinese Regional, has black wood from Nan Zhibei
The cultivation of ear.But sawdust used in the cultivation of black fungus is all the sawdust of non-coniferals, the sawdust of China a large amount of pine trees and cypress
It can only be the heap fermentation by many years at present, just can be carried out culture and utility after removing the pine and cypress resin contained in sawdust, thus
Cause the waste of a large amount of such sawdusts.Therefore how to cultivate can decompose using the black fungus strain of pine and cypress sawdust be solve this
One of the key factor of class sawdust waste.
Summary of the invention
Technical problem to be solved by the invention is to provide a kind of cultivations of the black fungus strain of decomposable pine and cypress tree sawdust
Method, this method simple process and low cost, for the black fungus strain of cultivation compared with conventional black fungus strain, having can be sufficiently sharp
With pine tree and cypress cultivation on sawdust black fungus, the advantages of a large amount of pine and cypress sawdusts waste is avoided, cultivation of auricularia auricula kind has in China
Uniqueness has a good application prospect.
A kind of cultural method of the black fungus strain of decomposable pine and cypress tree sawdust of the invention, comprising:
(1) bacterial context for the wild black fungus fructification being grown in pine and cypress tree or the spore of ejection are taken, under open environment
It is placed on preparatory prepared culture medium, culture medium is then placed in indoor culture 7~8 days of 25~26 DEG C, to mycelia
It grows；
(2) mycelia for obtaining step (1) is transferred in common PDA culture medium, and culture medium is then placed in 25~26
DEG C indoor culture 7~8 days, grown to mycelia；
(3) the mycelia switching obtained step (2) in prepared pine and cypress tree sawdust medium bacterium bag in advance (35cm ×
In 17cm), bacterium bag is then placed in indoor 30-40 days of 25~26 DEG C, is covered with to mycelia；
(4) mycelia that step (3) obtains is transferred in common PDA culture medium from pine and cypress tree sawdust bacterium bag, is placed in
25~26 DEG C of indoor culture 7~8 days.
Culture medium in the step (1) is PDA culture medium.
The group of common PDA culture medium in the step (2) becomes potato 200g filtering, glucose 20g and agar to boil water
20g。
The group of pine and cypress tree sawdust medium in the step (3) becomes pine and cypress sawdust 78wt%, wheat bran 20wt%, sugar
1wt% and gypsum 1wt%.
Beneficial effect
Present invention process is simple, at low cost, and for the black fungus strain of cultivation compared with conventional black fungus strain, having can be abundant
Using pine tree and cypress cultivation on sawdust black fungus, the advantages of a large amount of pine and cypress sawdusts waste is avoided, is had in China's cultivation of auricularia auricula kind
There is uniqueness, has a good application prospect.
Detailed description of the invention
Fig. 1 is wild pine tree black fungus fructification；
Fig. 2 is the black fungus strain of decomposable pine and cypress tree sawdust.
Specific embodiment
Present invention will be further explained below with reference to specific examples.It should be understood that these embodiments are merely to illustrate the present invention
Rather than it limits the scope of the invention.In addition, it should also be understood that, after reading the content taught by the present invention, those skilled in the art
Member can make various changes or modifications the present invention, and such equivalent forms equally fall within the application the appended claims and limited
Range.
Embodiment 1
(1) it takes the bacterial context for the wild black fungus fructification being grown in pine and cypress tree a little or the spore of ejection is a small amount of, opening
It puts and is placed under environment in preparatory prepared PDA culture medium, culture medium is then placed in indoor culture 7 days of 25 DEG C, to
Mycelia grows；
(2) the mycelia switching obtained step (1) is in common PDA culture medium (potato 200g filtering to boil water, glucose 20g
On agar 20g), culture medium is then placed in indoor culture 7 days of 25 DEG C, is grown to mycelia；
(3) the mycelia switching obtained step (2) is in preparatory prepared pine and cypress tree sawdust medium (pine and cypress sawdust
78wt%, wheat bran 20wt%, sugar 1wt% and gypsum 1wt%) in bacterium bag (35cm × 17cm), bacterium bag is then placed in 25 DEG C
Interior 30 days, covered with to mycelia；
(4) mycelia that step (3) obtains is transferred in common PDA culture medium from pine and cypress tree sawdust bacterium bag, is placed in
25 DEG C of indoor culture 7 days.
Embodiment 2
(1) it takes the bacterial context for the wild black fungus fructification being grown in pine and cypress tree a little or the spore of ejection is a small amount of, opening
It puts and is placed under environment in preparatory prepared PDA culture medium, culture medium is then placed in indoor culture 8 days of 26 DEG C, to
Mycelia grows；
(2) the mycelia switching obtained step (1) is in common PDA culture medium (potato 200g filtering to boil water, glucose 20g
On agar 20g), culture medium is then placed in indoor culture 7 days of 25 DEG C, is grown to mycelia；
(3) the mycelia switching obtained step (2) is in preparatory prepared pine and cypress tree sawdust medium (pine and cypress sawdust
78wt%, wheat bran 20wt%, sugar 1wt% and gypsum 1wt%) in bacterium bag (35cm × 17cm), bacterium bag is then placed in 26 DEG C
Interior 40 days, covered with to mycelia；
(4) mycelia that step (3) obtains is transferred in common PDA culture medium from pine and cypress tree sawdust bacterium bag, is placed in
26 DEG C of indoor culture 8 days.
Claims (4)
1. a kind of cultural method of the black fungus strain of decomposable pine and cypress tree sawdust, comprising:
(1) bacterial context for the wild black fungus fructification being grown in pine and cypress tree or the spore of ejection are taken, is placed under open environment
In on preparatory prepared culture medium, culture medium is then placed in indoor culture 7~8 days of 25~26 DEG C, is grown to mycelia；
(2) mycelia for obtaining step (1) is transferred in common PDA culture medium, and culture medium is then placed in 25~26 DEG C
Indoor culture 7~8 days, grows to mycelia；
(3) the mycelia switching obtained step (2) is in preparatory prepared pine and cypress tree sawdust medium bacterium bag, then by bacterium bag
It is placed in indoor 30-40 days of 25~26 DEG C, is covered with to mycelia；
(4) mycelia that step (3) obtains is transferred in common PDA culture medium from pine and cypress tree sawdust bacterium bag, it is placed in 25~
26 DEG C of indoor culture 7~8 days.
2. a kind of cultural method of the black fungus strain of decomposable pine and cypress tree sawdust according to claim 1, feature exist
In: the culture medium in the step (1) is PDA culture medium.
3. a kind of cultural method of the black fungus strain of decomposable pine and cypress tree sawdust according to claim 1, feature exist
In: the group of the common PDA culture medium in the step (2) becomes potato 200g filtering, glucose 20g and agar 20g to boil water.
4. a kind of cultural method of the black fungus strain of decomposable pine and cypress tree sawdust according to claim 1, feature exist
In: the group of the pine and cypress tree sawdust medium in the step (3) become pine and cypress sawdust 78wt%, wheat bran 20wt%, sugar 1wt% and
Gypsum 1wt%.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201610134506.0A CN105613049B (en) | 2016-03-09 | 2016-03-09 | A kind of cultural method of the black fungus strain of decomposable pine and cypress tree sawdust |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610134506.0A CN105613049B (en) | 2016-03-09 | 2016-03-09 | A kind of cultural method of the black fungus strain of decomposable pine and cypress tree sawdust |
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| CN105613049A CN105613049A (en) | 2016-06-01 |
| CN105613049B true CN105613049B (en) | 2018-12-14 |
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| CN201610134506.0A Active CN105613049B (en) | 2016-03-09 | 2016-03-09 | A kind of cultural method of the black fungus strain of decomposable pine and cypress tree sawdust |
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Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108901589A (en) * | 2018-04-18 | 2018-11-30 | 上海沃施生物科技有限公司 | A kind of separation method of wild delicious lactarius strain |
| CN113174338B (en) * | 2021-05-28 | 2022-12-20 | 广西壮族自治区农业科学院 | Black fungus tissue isolation culture medium and application thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101830747A (en) * | 2010-03-03 | 2010-09-15 | 上海市农业科学院 | Culture medium for use in artificial culture of Tibetan Yatung wild black fungi |
| CN102440150A (en) * | 2011-10-14 | 2012-05-09 | 姚安农哈哈食用菌开发有限公司 | Processing method for planting edible fungus by replacing hardwood forest wood chips with pine wood chips |
| CN105254407A (en) * | 2015-10-01 | 2016-01-20 | 西藏大学农牧学院 | Black fungus cultivation material and method |
| CN105265164A (en) * | 2014-07-22 | 2016-01-27 | 王健 | Method for cultivating edible mushrooms by pine/cedar/eucalyptus wood chips |
-
2016
- 2016-03-09 CN CN201610134506.0A patent/CN105613049B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101830747A (en) * | 2010-03-03 | 2010-09-15 | 上海市农业科学院 | Culture medium for use in artificial culture of Tibetan Yatung wild black fungi |
| CN102440150A (en) * | 2011-10-14 | 2012-05-09 | 姚安农哈哈食用菌开发有限公司 | Processing method for planting edible fungus by replacing hardwood forest wood chips with pine wood chips |
| CN105265164A (en) * | 2014-07-22 | 2016-01-27 | 王健 | Method for cultivating edible mushrooms by pine/cedar/eucalyptus wood chips |
| CN105254407A (en) * | 2015-10-01 | 2016-01-20 | 西藏大学农牧学院 | Black fungus cultivation material and method |
Non-Patent Citations (3)
| Title |
|---|
| 利用松、杉木屑栽培食用菌技术;范莉莉;《农家之友》;20031231(第19期);全文 * |
| 黑木耳松树锯末菌种及其栽培;夏温澍;《食用菌》;19790429;第26页配方比例 * |
| 黑木耳栽培技术和方法的研究;丁湖广;《林业科技通讯》;19870829;全文 * |
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