CN105199949A - Fluid control device of gene sequencing - Google Patents
Fluid control device of gene sequencing Download PDFInfo
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- CN105199949A CN105199949A CN201510585327.4A CN201510585327A CN105199949A CN 105199949 A CN105199949 A CN 105199949A CN 201510585327 A CN201510585327 A CN 201510585327A CN 105199949 A CN105199949 A CN 105199949A
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Abstract
The invention discloses a fluid control device of gene sequencing. The fluid control device of gene sequencing comprises a reagent assembly, a first multiported valve, a first three-port valve, a gene sequencing chip and a drive assembly; the reagent assembly is connected with the gene sequencing chip through the first multiported valve and the three-port valve; due to the fact that a first gene sequencing passage and a second gene sequencing passage are arranged in the gene sequencing chip, reagents in the reagent assembly can automatically flow into the first gene sequencing passage and the second gene sequencing passage to conduct reaction and fluorescence image acquisition, when fluorescent sequencing reaction is conducted in the first gene sequencing passage, fluorescence image acquisition can be conducted in the second gene sequencing passage, and the gene sequencing time can be effectively reduced, that is to say, the gene sequencing efficiency is effectively increased. For the fluid control device of gene sequencing, the gene sequencing cost is reduced, and the gene sequencing efficiency is increased.
Description
Technical field
The present invention relates to field of medical device, particularly relate to a kind of fluid control device of gene sequencing.
Background technology
Current single-molecule sequencing technology has three kinds of technological lines: single molecular fluorescence sequencing technologies, unit molecule synthesize sequencing technologies and nanoporous sequencing technologies in real time.Single molecular fluorescence sequencing technologies, have the advantages that accuracy rate is high, the order-checking time is short, the fluid control device of unit molecule gene sequencing is the key modules of unit molecule tester, and the gene chip in the fluid control device of unit molecule gene sequencing is the core component carrying out biochemical reaction and optical imagery, the fluid control device of gene sequencing arranges multiple fluid delivery device usually, and this fluid delivery device has the function such as mixing, derivation waste liquid of carrying various reaction reagent, the control reagent flow direction and flow, control reagent to gene chip.The mode of fluid control device many employings mechanical manipulator sample introduction needle of the gene sequencing of current commercialization is to the extraction of sequencing reagent or sample introduction, and sequencing reagent is consuming time longer in course of conveying, cause the efficiency of gene sequencing lower, and use cost is higher.
Summary of the invention
In view of this, the present invention is directed to the disappearance of prior art existence, its main purpose is to provide a kind of fluid control device improving the gene sequencing of gene sequencing efficiency.
For achieving the above object, the invention provides a kind of fluid control device of gene sequencing, wherein, the fluid control device of described gene sequencing comprises reagent assembly, first multiport valve, first T-valve, gene sequencing chip and driven unit, described reagent assembly comprises the sequencing reagent bottle of splendid attire gene sequencing reagent, the base reagent bottle of splendid attire fluorescence base excision reagent and the fluorescent reagent bottle of splendid attire fluorescence base reagent, described first multiport valve comprises the first extraction opening connecting described sequencing reagent bottle, connect the second extraction opening of described base reagent bottle and be connected to the 3rd extraction opening of described fluorescent reagent bottle, and a liquid outlet, described liquid outlet and described first extraction opening, or described second extraction opening, or described 3rd extraction opening is connected, described first T-valve comprises liquid sucting port, first diffluence pass and the second diffluence pass, described liquid sucting port connects described first diffluence pass or described second diffluence pass, described liquid sucting port pipeline connects described liquid outlet, described gene sequencing chip comprises the first gene sequencing passage and the second gene sequencing passage, described first gene sequencing passage and described second gene sequencing passage respectively pipeline are connected described first diffluence pass and described second diffluence pass, described driven unit comprises the first syringe pump and the second syringe pump, described first syringe pump and described second syringe pump respectively pipeline are connected described first gene sequencing passage and described second gene sequencing passage,
Wherein, negative pressure is provided to described first gene sequencing passage at described first syringe pump, with make described first gene sequencing passage obtain successively described gene sequencing reagent, fluorescence base reagent and fluorescent reagent carry out gene sequencing reaction time, described second syringe pump stops providing negative pressure to described second gene sequencing passage, carries out fluoroscopic image collection to make described second gene sequencing passage.
Wherein, the fluid control device of described gene sequencing also comprises sequencing reagent configuration component, described sequencing reagent configuration component comprises multiple source chemicals bottle, second multiport valve, second T-valve and the 3rd syringe pump, multiple described source chemicals bottle is in order to the multiple order-checking raw material of splendid attire, described second multiport valve simultaneously pipeline connects multiple described source chemicals bottle, and pipeline connects described second T-valve, described second T-valve also pipeline connects described 3rd syringe pump and described sequencing reagent bottle, described 3rd syringe pump is connected through described second T-valve and described second multiport valve and source chemicals bottle described in one of them, described sequencing reagent bottle is connected through described second T-valve and described 3rd syringe pump, wherein, described 3rd syringe pump is connected with multiple described source chemicals bottle successively, to extract the order-checking raw material in multiple described source chemicals bottle, and be mixedly configured into described gene sequencing reagent, described 3rd syringe pump and described sequencing reagent bottle are connected, for by described gene sequencing reagent injector to described sequencing reagent bottle.
Wherein, the first mixing tank is connected with between described second T-valve and described sequencing reagent bottle, described first mixing tank arranges multiple first serpentine tubes, and multiple described first serpentine tubes joins end to end, and connects between described second T-valve and described sequencing reagent bottle.
Wherein, the number of described sequencing reagent bottle is multiple, described sequencing reagent configuration component also comprises the 3rd multiport valve, described 3rd multiport valve simultaneously pipeline connects multiple described sequencing reagent bottle, and described second T-valve, described 3rd syringe pump is connected through described second T-valve and described 3rd multiport valve and sequencing reagent bottle described in one of them.
Wherein, described sequencing reagent configuration component also comprises wash reagent bottle and the first waste liquid bottle, described wash reagent bottle is used for splendid attire wash reagent, described wash reagent bottle is connected through described second multiport valve and described second T-valve and described 3rd syringe pump, described first waste liquid bottle is used for splendid attire waste liquid, and described first waste liquid bottle is connected through described 3rd multiport valve and described second T-valve and described 3rd syringe pump.
Wherein, the fluid control device of described gene sequencing also comprises fluorescent reagent configuration component, described fluorescent reagent configuration component comprises multiple fluorescence stock bottle, 4th multiport valve, 3rd T-valve and the 4th syringe pump, multiple described fluorescence stock bottle is in order to splendid attire multiple fluorescence base raw material, described 4th multiport valve simultaneously pipeline connects multiple described fluorescence stock bottle, and pipeline connects described 3rd T-valve, described 3rd T-valve also pipeline connects described 4th syringe pump and described fluorescent reagent bottle, described 4th syringe pump is connected through described 3rd T-valve and described 4th multiport valve and fluorescence stock bottle described in one of them, described fluorescent reagent bottle is connected through described 3rd multiport valve and described 4th syringe pump, wherein, described 4th syringe pump is connected with multiple described fluorescence stock bottle successively, to extract the fluorescence base raw material in multiple described fluorescence stock bottle, and be mixedly configured into fluorescence base reagent, described 4th syringe pump and described fluorescent reagent bottle are connected, for by described fluorescence base reagent injector in described fluorescent reagent bottle.
Wherein, described fluorescent reagent configuration component also comprises the second mixing tank, described second mixing tank is connected between described 3rd T-valve and described fluorescent reagent bottle, described second mixing tank comprises multiple second serpentine tubes, multiple described second serpentine tubes joins end to end, and connects between described 3rd T-valve and described fluorescent reagent bottle.
Wherein, described driven unit also comprises the 4th T-valve, the 5th T-valve, the second waste liquid bottle and the 3rd waste liquid bottle, described 4th T-valve pipeline is connected between described first syringe pump and described first gene sequencing passage, go back pipeline simultaneously and connect described second waste liquid bottle, described 5th T-valve pipeline is connected between described second syringe pump and described second gene sequencing passage, goes back pipeline simultaneously and connects described 3rd waste liquid bottle.
Wherein, the number of described first gene sequencing passage and described second gene sequencing passage is multiple.
Wherein, the fluid control device of described gene sequencing also comprises control unit, described control unit electrical connection described first multiport valve, the first T-valve, gene sequencing chip and described driven unit, run to control described first multiport valve, the first T-valve, gene sequencing chip and described driven unit.
The fluid control device of gene sequencing provided by the invention, be connected with described gene sequencing chip with described first T-valve through described first multiport valve by described reagent assembly, and utilize in described gene chip described first gene sequencing passage and described second gene sequencing passage are set, thus the reagent of described reagent assembly can be flowed in described first gene sequencing passage and described second gene sequencing passage automatically carry out reaction and fluoroscopic image collection, and when described first gene sequencing passage carries out fluorescence sequencing reaction, described second gene sequencing passage can carry out fluoroscopic image collection, thus effectively reduce the gene sequencing time, namely effectively improve gene sequencing efficiency, the fluid control device of described gene sequencing is made to decrease gene sequencing cost, improve gene sequencing efficiency.
Accompanying drawing explanation
For more clearly setting forth structural attitude of the present invention and effect, be described in detail below in conjunction with accompanying drawing and specific embodiment.
Fig. 1 is the structural representation of the fluid control device of gene sequencing provided by the invention.
Embodiment
Below in conjunction with the accompanying drawing in embodiment of the present invention, the technical scheme in embodiment of the present invention is clearly and completely described.
See also Fig. 1, the fluid control device 100 of a kind of gene sequencing provided by the invention.The fluid control device 100 of described gene sequencing comprises reagent assembly, the first multiport valve 20, first T-valve 30, gene sequencing chip 40 and driven unit 50.Described reagent assembly comprises the fluorescent reagent bottle 13 of the sequencing reagent bottle 11 of splendid attire gene sequencing reagent, the base reagent bottle 12 of splendid attire fluorescence base excision reagent and splendid attire fluorescence base reagent.Described first multiport valve 20 comprise connect described sequencing reagent bottle 11 the first extraction opening 21, connect the second extraction opening 22 of described base reagent bottle 12 and be connected to the 3rd extraction opening 23 of described fluorescent reagent bottle 13, and one order-checking liquid outlet 24.Described liquid outlet 24 and described first extraction opening 21 or described second extraction opening 22 or described 3rd extraction opening 23 are connected.Described first T-valve 30 comprises liquid sucting port 31, first diffluence pass 32 and the second diffluence pass 33, and described liquid sucting port 31 connects described first diffluence pass 32 or described second diffluence pass 33.Described liquid sucting port 31 pipeline connects described liquid outlet 24.Described gene sequencing chip 40 comprises the first gene sequencing passage 41 and the second gene sequencing passage 42, and described first gene sequencing passage 41 and described second gene sequencing passage 42 respectively pipeline are connected described first diffluence pass 32 and described second diffluence pass 33.Described driven unit 50 comprises the first syringe pump 51 and the second syringe pump 52, and described first syringe pump 51 and described second syringe pump 52 respectively pipeline are connected described first gene sequencing passage 41 and described second gene sequencing passage 42;
Wherein, negative pressure is provided to described first gene sequencing passage 41 at described first syringe pump 51, with make described first gene sequencing passage 41 obtain successively described gene sequencing reagent, fluorescence base reagent and fluorescent reagent carry out gene sequencing reaction time, described second syringe pump 52 stops providing negative pressure to described second gene sequencing passage 42, carries out fluoroscopic image collection to make described second gene sequencing passage 42.
Be connected with described gene sequencing chip 40 with described first T-valve 30 through described first multiport valve 20 by described reagent assembly, and utilize in described gene chip 40 described first gene sequencing passage 41 and described second gene sequencing passage 42 are set, thus the reagent of described reagent assembly can be flowed in described first gene sequencing passage 41 and described second gene sequencing passage 42 automatically carry out reaction and fluoroscopic image collection, and when described first gene sequencing passage 41 carries out fluorescence sequencing reaction, described second gene sequencing passage 42 can carry out fluoroscopic image collection, thus effectively reduce the gene sequencing time, namely effectively improve gene sequencing efficiency, the fluid control device 100 of described gene sequencing is made to decrease gene sequencing cost, improve gene sequencing efficiency.
In present embodiment, the opening conduit of described sequencing reagent bottle 11 is connected to described first extraction opening 21.Described sequencing reagent bottle 11 adopts transparent glass test tube to make, the opening of described sequencing reagent bottle 11 is arranged at the top of described sequencing reagent bottle 11, opening is plugged with plug (mark), plug is inserted with tubing (mark), described tubing all extend into the bottom of described sequencing reagent bottle 11, thus fully can extract the gene sequencing reagent of described sequencing reagent bottle 11.In other embodiments, described sequencing reagent bottle 10 can also be box body.
Described base reagent bottle 12 is identical with the structure of described sequencing reagent bottle 11 with described fluorescent reagent bottle 13, does not repeat them here.
In present embodiment, described first multiport valve 20 is rotary valve, described first extraction opening 21, second extraction opening 22 and described 3rd extraction opening 23 are respectively around described liquid outlet 24, and described first extraction opening 21, second extraction opening 22 and described 3rd extraction opening 23 connect described liquid outlet 24 by the rotating pipe 25 rotated around described liquid outlet 24.Described rotating pipe 25 can rotate the position to described first extraction opening 21, second extraction opening 22 and the 3rd extraction opening 23 successively, thus realize described liquid outlet 24 and can connect described sequencing reagent bottle 11, described base reagent bottle 12 and described fluorescent reagent bottle 13 successively, namely described gene sequencing chip 30 can obtain reagent from described sequencing reagent bottle 11, described base reagent bottle 12 and described fluorescent reagent bottle 13 successively, and then gene sequencing reaction and fluoroscopic image collection.In other embodiments, described liquid outlet 24 also can not limit with the connection order of described first extraction opening 21, second extraction opening 22 and the 3rd extraction opening 23.
In present embodiment, when liquid sucting port 31 and described first diffluence pass 32 of described first T-valve 30 are connected, described liquid sucting port 31 disconnects with described second diffluence pass 33.Described liquid sucting port 31 can be connect described first diffluence pass 32 or described second diffluence pass 33 according to the gene sequencing cycle, namely when described first gene sequencing passage 41 carries out gene sequencing reaction in one-period, described first diffluence pass 32 is connected with described liquid sucting port 30, thus described liquid sucting port 30 provides reaction reagent through described first diffluence pass 32 to described first gene sequencing passage 41, and now described second diffluence pass 33 disconnects with described liquid sucting port 31, thus make the reaction reagent in described second gene sequencing passage 42 stop flowing, thus realize fluoroscopic image collection and take pictures, complete after fluoroscopic image collection until described second gene sequencing passage 42, described second diffluence pass 33 is connected with described liquid sucting port 31, described second gene sequencing passage 42 starts to obtain reaction reagent through described liquid sucting port 31, and carry out gene sequencing reaction, described 41, first gene sequencing passage stops obtaining reaction reagent, and carry out fluoroscopic image collection, thus effectively reduce the gene sequencing time, improve gene sequencing efficiency.
In present embodiment, the two ends difference pipeline of described first gene sequencing passage 41 connects the first diffluence pass 31 of described first T-valve 30 and described first syringe pump 51.The two ends difference pipeline of described second gene sequencing passage 42 connects the second diffluence pass 32 of described first T-valve 30 and described second syringe pump 52.When carrying out gene sequencing reaction in described first gene sequencing passage 41, carry out fluoroscopic image collection in described second gene sequencing passage 42 and take pictures; When carrying out gene sequencing reaction in described second gene sequencing passage 42, described first gene sequencing passage 41 can carry out fluoroscopic image collection and take pictures, thus described first gene sequencing passage 41 and the coordinated operation mutually of described second gene sequencing passage 42, thus effectively reduce the fluorogene order-checking time, thus improve gene sequencing efficiency.
In present embodiment, described first syringe pump 51 and described second syringe pump 52 utilize the order-checking waste liquid having completed gene sequencing reaction in the first gene sequencing passage 41 described in negative pressure extracting and described second gene sequencing passage 42 respectively, thus ensure that gene sequencing reaction is stable and carry out.
Further, the fluid control device 100 of described gene sequencing also comprises sequencing reagent configuration component 60, and described sequencing reagent configuration component 60 comprises multiple source chemicals bottle 61, second multiport valve 62, second T-valve 63 and the 3rd syringe pump 64.Multiple described source chemicals bottle 61 is in order to the multiple order-checking raw material of splendid attire, and described second multiport valve 62 simultaneously pipeline connects multiple described source chemicals bottle 61, and pipeline connects described second T-valve 63.Described second T-valve 63 is gone back pipeline and is connected described 3rd syringe pump 64 and described sequencing reagent bottle 11.Described 3rd syringe pump 64 is connected with source chemicals bottle 61 described in one of them through described second T-valve 63 and described second multiport valve 62.Described sequencing reagent bottle 61 is connected with described 3rd syringe pump 64 through described second T-valve 63.Wherein, described 3rd syringe pump 64 is connected with multiple described source chemicals bottle 61 successively, to extract the order-checking raw material in multiple described source chemicals bottle 61, and be mixedly configured into described gene sequencing reagent, described 3rd syringe pump 64 is connected with described sequencing reagent bottle 11, for by described gene sequencing reagent injector to described sequencing reagent bottle 11.
In present embodiment, multiple described source chemicals bottle 61 gene sequencing raw material that splendid attire is different respectively, thus described 3rd syringe pump 64 can be utilized to extract gene sequencing raw material in multiple described source chemicals bottle 61 successively, thus be mixedly configured into gene sequencing reagent.Concrete, the number of described source chemicals bottle 61 is ten.The structure of described source chemicals bottle 61 and the identical setting of structure of described sequencing reagent bottle 11.Multiple described source chemicals bottle 61 can be prevent terminating on test-tube stand, to carry out firmly to multiple described source chemicals bottle 61, can also to be sticked different label to six described source chemicals bottles 61 respectively simultaneously, carry out order-checking raw material next time supplement to facilitate, the cross infection of raw material of avoiding checking order.In other embodiments, the number of described source chemicals bottle 61 can also be six or eight.
The identical setting of structure of described second multiport valve 62 and described first multiport valve 20.Unlike, described second multiport valve 62 realizes described 3rd syringe pump 64 and is connected with multiple described source chemicals bottle 61 successively, described second multiport valve 62 is selected source chemicals bottle 61 described in one of them and is connected, and by controlling to connect duration, thus control the adjustment of described 3rd syringe pump 64 to order-checking raw material extraction amount in this source chemicals bottle 61.Thus the order-checking raw material realizing multiple described source chemicals bottle 61 can be configured in proportion, to meet gene sequencing demand.
Described second T-valve 63 and the identical setting of described first T-valve 30 structure.Described second T-valve 63 can realize described 3rd syringe pump 64 and described second multiport valve 62 is connected, thus described 3rd syringe pump 64 can extract the gene sequencing raw material in multiple described source chemicals bottle 61, is configured to gene sequencing reagent.Described second T-valve 63 can realize described 3rd syringe pump 64 and described sequencing reagent bottle 11 is connected, thus described 3rd syringe pump 64 can inject the gene sequencing reagent configured in described sequencing reagent bottle 11.
Described 3rd syringe pump 64 can provide negative pressure through described second T-valve 63 and described second multiport valve 62 to multiple described source chemicals bottle 61, to extract the gene sequencing reagent in multiple described source chemicals bottle 61.Described 3rd syringe pump 64 can also provide malleation through described second T-valve 63 in described sequencing reagent bottle 11, to inject in gene sequencing reagent to described sequencing reagent bottle 11.
Further, the first mixing tank 65 is connected with between described second T-valve 63 and described sequencing reagent bottle 11, described first mixing tank 65 arranges multiple first serpentine tubes 651, multiple described first serpentine tubes 651 joins end to end, and connects between described second T-valve 63 and described sequencing reagent bottle 11.
In present embodiment, multiple described first serpentine tubes 651 is fixed on a retaining plate, and described first serpentine tubes 651 is S-shaped, and multiple described serpentine tubes 651 can be that many rows are arranged side by side, and often mutually connects between row.Multiple described first serpentine tubes 651 is utilized to connect between the good described sequencing reagent bottle 11 of described second T-valve 63, the gene sequencing reagent injected from described 3rd syringe pump 64 is cushioned through multiple described serpentine tubes 651, and increase the flowing stroke of gene sequencing reagent, thus the order-checking of the several genes in gene sequencing reagent raw material is fully mixed, promote gene sequencing reagent react efficiency.In other embodiments, multiple described serpentine tubes 651 can also be spiral successively.
Further, the number of described sequencing reagent bottle 11 is multiple, described sequencing reagent configuration component 60 also comprises the 3rd multiport valve 66, described 3rd multiport valve 66 simultaneously pipeline connects multiple described sequencing reagent bottle 11, and described second T-valve 63, described 3rd syringe pump 64 is connected with sequencing reagent bottle 11 described in one of them through described second T-valve 63 and described 3rd multiport valve 66.
In present embodiment, the sequencing reagent in multiple described sequencing reagent bottle 11 is different, and the number of described sequencing reagent bottle 11 is four.The order-checking source chemicals ratio extracting multiple described source chemicals bottle 61 according to described 3rd syringe pump 64 is different, can be configured to different gene sequencing reagent, thus can utilize the multiple different gene sequencing reagent of multiple described sequencing reagent bottle 11 splendid attires.Described 3rd multiport valve 66 structure and the identical setting of described second multiport valve 62 structure.Described 3rd multiport valve 60 can realize described 3rd syringe pump 64 and in multiple described sequencing reagent bottle 11, inject different gene sequencing reagent respectively successively.Concrete, after described 3rd syringe pump 64 has often configured gene sequencing reagent, through described second T-valve 63 and the selected described sequencing reagent bottle 11 of described 3rd multiport valve 60, and by gene sequencing reagent injector in this sequencing reagent bottle 11.In other embodiments, the number of described sequencing reagent bottle 11 can also be five, six or seven.
Further, described sequencing reagent configuration component 60 also comprises wash reagent bottle 67 and the first waste liquid bottle 68, described wash reagent bottle 67 is for splendid attire wash reagent, described wash reagent bottle 67 is connected with described 3rd syringe pump 64 through described second multiport valve 63 and described second T-valve 62, described first waste liquid bottle 68 is for splendid attire waste liquid, and described first waste liquid bottle 68 is connected with described 3rd syringe pump 64 through described 3rd multiport valve 66 and described second T-valve 62.
Described wash reagent bottle 67 is when described second multiport valve 63 and described second T-valve 62 are connected with described 3rd syringe pump 64, described 3rd syringe pump 64 can extract wash reagent in described wash reagent bottle 67, to rinse described 3rd syringe pump 64, namely described 3rd syringe pump 64 is after configuration completes a kind of gene sequencing reagent, before configuring gene sequencing reagent next time, first can extract the wash reagent in described wash reagent bottle 67, gene sequencing reagent is again configured after rinsing, thus avoid the different gene sequencing of configuration two kinds to occur cross infection.Described first waste liquid bottle 68 is when described 3rd multiport valve 66 and described second T-valve 62 are connected with described 3rd syringe pump 64; the waste liquid completing cleaning can be injected in described first waste liquid bottle 68 by described 3rd syringe pump 64, thus reaches the effect of environmental protection recovery.
Further, the fluid control device 100 of described gene sequencing also comprises fluorescent reagent configuration component 70, and described fluorescent reagent configuration component 70 comprises multiple fluorescence stock bottle 71, the 4th multiport valve 72, the 3rd T-valve 73 and the 4th syringe pump 74.Multiple described fluorescence stock bottle 71 is in order to splendid attire multiple fluorescence base raw material.Described 4th multiport valve 72 simultaneously pipeline connects multiple described fluorescence stock bottle 71, and pipeline connects described 3rd T-valve 73.Described 3rd T-valve 73 is gone back pipeline and is connected described 4th syringe pump 74 and described fluorescent reagent bottle 13.Described 4th syringe pump 74 is connected with fluorescence stock bottle 71 described in one of them through described 3rd T-valve 73 and described 4th multiport valve 72.Described fluorescent reagent bottle 13 is connected with described 4th syringe pump 74 through described 4th multiport valve 72, wherein, described 4th syringe pump 74 is connected with multiple described fluorescence stock bottle 71 successively, to extract the fluorescence base raw material in multiple described fluorescence stock bottle 71, and be mixedly configured into fluorescence base reagent.Described 4th syringe pump 74 is connected with described fluorescent reagent bottle 13, for by described fluorescence base reagent injector to described fluorescent reagent bottle 13.
In present embodiment, multiple described fluorescence stock bottle 71 fluorescence base raw material that splendid attire is different respectively, thus described 4th syringe pump 74 can be utilized to extract gene sequencing raw material in multiple described fluorescence stock bottle 71 successively, thus be mixedly configured into fluorescence base reagent.Concrete, the number of described fluorescence stock bottle 71 is five.The structure of described fluorescence stock bottle 71 and the identical setting of structure of described sequencing reagent bottle 11.Multiple described fluorescence source chemicals bottle 71 can be positioned on test-tube stand, to carry out firmly to multiple described fluorescence stock bottle 71, can also to be sticked different label to five described fluorescence stock bottles 71 respectively simultaneously, carry out fluorescence source chemicals next time supplement to facilitate, avoid the cross infection of fluorescence raw material.In other embodiments, the number of described fluorescence stock bottle 71 can also be six or eight.
The identical setting of structure of described 4th multiport valve 72 and described first multiport valve 20.Unlike, described 4th multiport valve 72 realizes described 4th syringe pump 74 and is connected with multiple described fluorescence stock bottle 71 successively, described 4th multiport valve 72 is selected fluorescence stock bottle 71 described in one of them and is connected, and by controlling to connect duration, thus control the adjustment of described 4th syringe pump 74 to fluorescence base raw material extraction amount in this fluorescence stock bottle 71.Thus the fluorescence base raw material realizing multiple described fluorescence stock bottle 71 can be configured in proportion, to meet gene sequencing demand.
Described 3rd T-valve 73 and the identical setting of described first T-valve 30 structure.Described 3rd T-valve 73 can realize described 4th syringe pump 74 and described 4th multiport valve 72 is connected, thus described 4th syringe pump 74 can extract the fluorescence base raw material in multiple described fluorescence stock bottle 71, is configured to fluorescence base reagent.Described 3rd T-valve 73 can realize described 4th syringe pump 74 and described fluorescent reagent bottle 71 is connected, thus described 4th syringe pump 74 can inject the fluorescence base reagent configured in described fluorescent reagent bottle 13.
Described 4th syringe pump 74 can provide negative pressure to multiple described fluorescence source chemicals bottle 71, to extract the gene sequencing reagent in multiple described fluorescence stock bottle 71 through described 3rd T-valve 73 and described 4th multiport valve 72.Described 4th syringe pump 74 can also provide malleation through described 3rd T-valve 73 in described fluorescent reagent bottle 71, to inject gene sequencing reagent in described fluorescence order-checking bottle 71.
Further, described fluorescent reagent configuration component 70 also comprises the second mixing tank 75, described second mixing tank 75 is connected between described 3rd T-valve 73 and described fluorescent reagent bottle 13, described second mixing tank 75 comprises multiple second serpentine tubes 751, multiple described second serpentine tubes 751 joins end to end, and connects between described 3rd T-valve 73 and described fluorescent reagent bottle 13.
Described second mixing tank 75 and the identical setting of described first mixing tank 65 structure, the fluorescence base reagent that described second mixing tank 75 injects from described 4th syringe pump 74 cushions through multiple described second serpentine tubes 751, and increase the flowing stroke of fluorescence base sequence reagent, thus the multiple fluorescence base raw material in fluorescence base reagent is fully mixed, promote gene sequencing reagent react efficiency.
Further, described driven unit 50 also comprises the 4th T-valve 53, the 5th T-valve 54, second waste liquid bottle 55 and the 3rd waste liquid bottle 56.Described 4th T-valve 53 pipeline is connected between described first syringe pump 51 and described first gene sequencing passage 41, goes back pipeline simultaneously and connects described second waste liquid bottle 55.Described 5th T-valve 54 pipeline is connected between described second syringe pump 52 and described second gene sequencing passage 42, goes back pipeline simultaneously and connects described 3rd waste liquid bottle 56.
Described first syringe pump 51 connects described first gene sequencing passage 41 or described second waste liquid bottle 55 through described 4th T-valve 53, thus after described first syringe pump 51 extracts and completed the waste liquid of gene sequencing reaction in described first gene sequencing passage 41, waste liquid can be injected to described second waste liquid bottle 55, thus make described first syringe pump 51 carry out providing negative pressure, to carry out gene sequencing reaction to described first gene sequencing passage 41 next time.Described 5th T-valve 54 and the identical setting of described 4th T-valve 53 structure, do not repeat them here, and described 3rd waste liquid bottle 56 and the identical setting of described second waste liquid bottle 55 structure, do not repeat them here.
Further, the number of described first gene sequencing passage 41 and described second gene sequencing passage 42 is multiple.Utilize the number of described first gene sequencing passage 41 and described second gene sequencing passage 42 all to arrange multiple, multiple gene sequencing reaction can be made simultaneously to carry out, thus improve gene sequencing efficiency further.Same principle, the number of described first syringe pump 51 and described second syringe pump 52 also can be multiple, multiple described first syringe pump 51 correspondence provides negative pressure to multiple described first gene sequencing passage 41, and multiple described second syringe pump 52 correspondence provides negative pressure to multiple described second gene sequencing passage 42.Multiple described first syringe pump 51 and multiple described second syringe pump 52 can be adopt the mode of townhouse braking to drive, thus multiple described first syringe pump 51 or multiple described second syringe pump 52 can be driven simultaneously, thus ensure the accuracy of described gene sequencing efficiency.
Further, the fluid control device 100 of described gene sequencing also comprises control unit (not shown), described control unit electrical connection described first multiport valve 20, first T-valve 30, gene sequencing chip 40 and described driven unit 50, run to control described first multiport valve 20, first T-valve 30, gene sequencing chip 40 and described driven unit 50.Described control unit can be micro-chip, counter treater or central control processor etc., utilize described control unit to control described first multiport valve 20, first T-valve 30, gene sequencing chip 40 and described driven unit 50 to run, the fluid control device 100 realizing described gene sequencing runs automatically, puts on gene side draught efficiency.In present embodiment, described control unit 100 is also electrically connected described second multiport valve 62, second T-valve 63, the 3rd multiport valve 66, the 4th multiport valve 72, the 3rd T-valve 73, the 3rd syringe pump 64 and the 4th syringe pump 74, and fluid control device 100 operational efficiency of described gene sequencing is promoted.
The fluid control device of gene sequencing provided by the invention, be connected with described gene sequencing chip with described first T-valve through described first multiport valve by described reagent assembly, and utilize in described gene chip described first gene sequencing passage and described second gene sequencing passage are set, thus the reagent of described reagent assembly can be flowed in described first gene sequencing passage and described second gene sequencing passage automatically carry out reaction and fluoroscopic image collection, and when described first gene sequencing passage carries out fluorescence sequencing reaction, described second gene sequencing passage can carry out fluoroscopic image collection, thus effectively reduce the gene sequencing time, namely effectively improve gene sequencing efficiency, the fluid control device of described gene sequencing is made to decrease gene sequencing cost, improve gene sequencing efficiency.
The above is the preferred embodiment of the present invention; it should be pointed out that for those skilled in the art, under the premise without departing from the principles of the invention; can also make some improvements and modifications, these improvements and modifications are also considered as protection scope of the present invention.
Claims (10)
1. the fluid control device of a gene sequencing, it is characterized in that, the fluid control device of described gene sequencing comprises reagent assembly, first multiport valve, first T-valve, gene sequencing chip and driven unit, described reagent assembly comprises the sequencing reagent bottle of splendid attire gene sequencing reagent, the base reagent bottle of splendid attire fluorescence base excision reagent and the fluorescent reagent bottle of splendid attire fluorescence base reagent, described first multiport valve comprises the first extraction opening connecting described sequencing reagent bottle, connect the second extraction opening of described base reagent bottle and be connected to the 3rd extraction opening of described fluorescent reagent bottle, and a liquid outlet, described liquid outlet and described first extraction opening, or described second extraction opening, or described 3rd extraction opening is connected, described first T-valve comprises liquid sucting port, first diffluence pass and the second diffluence pass, described liquid sucting port connects described first diffluence pass or described second diffluence pass, described liquid sucting port pipeline connects described liquid outlet, described gene sequencing chip comprises the first gene sequencing passage and the second gene sequencing passage, described first gene sequencing passage and described second gene sequencing passage respectively pipeline are connected described first diffluence pass and described second diffluence pass, described driven unit comprises the first syringe pump and the second syringe pump, described first syringe pump and described second syringe pump respectively pipeline are connected described first gene sequencing passage and described second gene sequencing passage,
Wherein, negative pressure is provided to described first gene sequencing passage at described first syringe pump, with make described first gene sequencing passage obtain successively described gene sequencing reagent, fluorescence base reagent and fluorescent reagent carry out gene sequencing reaction time, described second syringe pump stops providing negative pressure to described second gene sequencing passage, carries out fluoroscopic image collection to make described second gene sequencing passage.
2. the fluid control device of gene sequencing according to claim 1, it is characterized in that, the fluid control device of described gene sequencing also comprises sequencing reagent configuration component, described sequencing reagent configuration component comprises multiple source chemicals bottle, second multiport valve, second T-valve and the 3rd syringe pump, multiple described source chemicals bottle is in order to the multiple order-checking raw material of splendid attire, described second multiport valve simultaneously pipeline connects multiple described source chemicals bottle, and pipeline connects described second T-valve, described second T-valve also pipeline connects described 3rd syringe pump and described sequencing reagent bottle, described 3rd syringe pump is connected through described second T-valve and described second multiport valve and source chemicals bottle described in one of them, described sequencing reagent bottle is connected through described second T-valve and described 3rd syringe pump, wherein, described 3rd syringe pump is connected with multiple described source chemicals bottle successively, to extract the order-checking raw material in multiple described source chemicals bottle, and be mixedly configured into described gene sequencing reagent, described 3rd syringe pump and described sequencing reagent bottle are connected, for by described gene sequencing reagent injector to described sequencing reagent bottle.
3. the fluid control device of gene sequencing according to claim 2, it is characterized in that, the first mixing tank is connected with between described second T-valve and described sequencing reagent bottle, described first mixing tank arranges multiple first serpentine tubes, multiple described first serpentine tubes joins end to end, and connects between described second T-valve and described sequencing reagent bottle.
4. the fluid control device of gene sequencing according to claim 2, it is characterized in that, the number of described sequencing reagent bottle is multiple, described sequencing reagent configuration component also comprises the 3rd multiport valve, described 3rd multiport valve simultaneously pipeline connects multiple described sequencing reagent bottle, and described second T-valve, described 3rd syringe pump is connected through described second T-valve and described 3rd multiport valve and sequencing reagent bottle described in one of them.
5. the fluid control device of gene sequencing according to claim 4, it is characterized in that, described sequencing reagent configuration component also comprises wash reagent bottle and the first waste liquid bottle, described wash reagent bottle is used for splendid attire wash reagent, described wash reagent bottle is connected through described second multiport valve and described second T-valve and described 3rd syringe pump, described first waste liquid bottle is used for splendid attire waste liquid, and described first waste liquid bottle is connected through described 3rd multiport valve and described second T-valve and described 3rd syringe pump.
6. the fluid control device of gene sequencing according to claim 1, it is characterized in that, the fluid control device of described gene sequencing also comprises fluorescent reagent configuration component, described fluorescent reagent configuration component comprises multiple fluorescence stock bottle, 4th multiport valve, 3rd T-valve and the 4th syringe pump, multiple described fluorescence stock bottle is in order to splendid attire multiple fluorescence base raw material, described 4th multiport valve simultaneously pipeline connects multiple described fluorescence stock bottle, and pipeline connects described 3rd T-valve, described 3rd T-valve also pipeline connects described 4th syringe pump and described fluorescent reagent bottle, described 4th syringe pump is connected through described 3rd T-valve and described 4th multiport valve and fluorescence stock bottle described in one of them, described fluorescent reagent bottle is connected through described 3rd multiport valve and described 4th syringe pump, wherein, described 4th syringe pump is connected with multiple described fluorescence stock bottle successively, to extract the fluorescence base raw material in multiple described fluorescence stock bottle, and be mixedly configured into fluorescence base reagent, described 4th syringe pump and described fluorescent reagent bottle are connected, for by described fluorescence base reagent injector in described fluorescent reagent bottle.
7. the fluid control device of gene sequencing according to claim 6, it is characterized in that, described fluorescent reagent configuration component also comprises the second mixing tank, described second mixing tank is connected between described 3rd T-valve and described fluorescent reagent bottle, described second mixing tank comprises multiple second serpentine tubes, multiple described second serpentine tubes joins end to end, and connects between described 3rd T-valve and described fluorescent reagent bottle.
8. the fluid control device of gene sequencing according to claim 1, it is characterized in that, described driven unit also comprises the 4th T-valve, the 5th T-valve, the second waste liquid bottle and the 3rd waste liquid bottle, described 4th T-valve pipeline is connected between described first syringe pump and described first gene sequencing passage, go back pipeline simultaneously and connect described second waste liquid bottle, described 5th T-valve pipeline is connected between described second syringe pump and described second gene sequencing passage, goes back pipeline simultaneously and connects described 3rd waste liquid bottle.
9. the fluid control device of gene sequencing according to claim 1, is characterized in that, the number of described first gene sequencing passage and described second gene sequencing passage is multiple.
10. the fluid control device of gene sequencing according to claim 1, it is characterized in that, the fluid control device of described gene sequencing also comprises control unit, described control unit electrical connection described first multiport valve, the first T-valve, gene sequencing chip and described driven unit, run to control described first multiport valve, the first T-valve, gene sequencing chip and described driven unit.
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| CN201510585327.4A CN105199949B (en) | 2015-09-15 | 2015-09-15 | The fluid control device of gene sequencing |
| PCT/CN2016/098890 WO2017045587A1 (en) | 2015-09-15 | 2016-09-13 | Fluid control device for gene sequencing |
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| CN201510585327.4A CN105199949B (en) | 2015-09-15 | 2015-09-15 | The fluid control device of gene sequencing |
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| CN105199949B CN105199949B (en) | 2017-12-12 |
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| WO2017045587A1 (en) * | 2015-09-15 | 2017-03-23 | 深圳市瀚海基因生物科技有限公司 | Fluid control device for gene sequencing |
| CN107828641A (en) * | 2017-08-23 | 2018-03-23 | 苏州思维医疗科技有限公司 | A kind of gene sequencer |
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| CN112326652A (en) * | 2017-09-20 | 2021-02-05 | 深圳市真迈生物科技有限公司 | Imaging method, device and system for controlling sequence determination reaction |
| CN112326652B (en) * | 2017-09-20 | 2021-09-03 | 深圳市真迈生物科技有限公司 | Imaging method, device and system for controlling sequence determination reaction |
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Also Published As
| Publication number | Publication date |
|---|---|
| CN105199949B (en) | 2017-12-12 |
| WO2017045587A1 (en) | 2017-03-23 |
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