CN111040942B - Gene sequencing device and gene sequencing method - Google Patents
Gene sequencing device and gene sequencing method Download PDFInfo
- Publication number
- CN111040942B CN111040942B CN201911231595.0A CN201911231595A CN111040942B CN 111040942 B CN111040942 B CN 111040942B CN 201911231595 A CN201911231595 A CN 201911231595A CN 111040942 B CN111040942 B CN 111040942B
- Authority
- CN
- China
- Prior art keywords
- solution cavity
- conveying channel
- solution
- sample
- cavity
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/483—Physical analysis of biological material
- G01N33/487—Physical analysis of biological material of liquid biological material
- G01N33/48707—Physical analysis of biological material of liquid biological material by electrical means
- G01N33/48721—Investigating individual macromolecules, e.g. by translocation through nanopores
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A90/00—Technologies having an indirect contribution to adaptation to climate change
- Y02A90/10—Information and communication technologies [ICT] supporting adaptation to climate change, e.g. for weather forecasting or climate simulation
Abstract
The invention discloses a gene sequencing device and a gene sequencing method. The gene sequencing device comprises: a storage unit for storing a reagent; the first conveying channel is connected with the storage unit and the waste liquid bottle to form a straight-through flow path; the solution cavity input end is used for communicating a solution cavity of the chip to be tested and feeding liquid into the solution cavity; the solution cavity output end is used for communicating a solution cavity of the chip to be tested, and liquid is discharged from the solution cavity; the sample injection unit comprises a sample injection port and a temporary storage cavity which are sequentially connected; the second conveying channel is connected with the temporary storage cavity and the input end of the solution cavity; the third conveying channel is connected with the storage unit and the input end of the solution cavity; the fourth conveying channel is connected with the output end of the solution cavity and the waste liquid bottle; the fixing unit is used for fixing the chip to be tested; and the pump body is used for enabling the reagent and the sample liquid to flow through the solution cavity for reaction. Through setting up a plurality of conveying channels, form different flow paths, accomplish pretreatment operations under the control of the pump body, avoid manual intervention to the influence of detection accuracy, reduce the technical requirement of detection.
Description
Technical Field
The invention relates to the technical field of sequencing, in particular to a gene sequencing device and a gene sequencing method.
Background
Gene sequencing is a novel gene detection technology capable of analyzing and measuring gene sequences from blood, saliva, hair or mucous membrane and predicting possibility of suffering from various diseases, individual behavior characteristics, behavior rationality and the like. Gene sequencing technology can lock individual lesion genes, intervene or treat in advance. The development of the gene sequencing technology has been that the corresponding gene sequencer is subjected to the change of over-the-earth, the aspects of flux, sequencing accuracy, sequencing period, reading length and the like are continuously improved, the application is more and more extensive, and the most critical parts of the gene sequencing are also mainly finished by depending on the gene sequencing chip. In recent years, research on gene sequencing has been conducted around the development of gene chips, and gene sequencers applied to clinical or research have been mainly based on medium-and large-sized sequencers, and prior to formal testing, professional technicians are required to perform complicated manual pretreatment operations, so that the technical requirements are relatively high.
Disclosure of Invention
The present invention aims to solve at least one of the technical problems existing in the prior art. Therefore, the invention provides a gene sequencing device, and the system can be used for carrying out gene sequencing without artificial pretreatment, so that the technical requirement of detection is reduced.
The invention also provides a gene sequencing method of the gene sequencing device.
In a first aspect, an embodiment of the present invention provides a gene sequencing device comprising:
a storage unit for storing a reagent;
the first conveying channel is connected with the storage unit and the waste liquid bottle to form a straight-through flow path;
the solution cavity input end is used for communicating a solution cavity of the chip to be tested and feeding liquid into the solution cavity;
the solution cavity output end is used for communicating a solution cavity of the chip to be tested, and liquid is discharged from the solution cavity;
the sample injection unit comprises a sample injection port and a temporary storage cavity which are sequentially connected;
the second conveying channel is connected with the temporary storage cavity and the input end of the solution cavity;
the third conveying channel is connected with the storage unit and the input end of the solution cavity;
the fourth conveying channel is connected with the waste liquid bottle and the output end of the solution cavity;
the fixing unit is used for fixing the chip to be tested;
and the pump body is used for enabling the reagent and the sample liquid to flow through the solution cavity for reaction.
The gene sequencing device provided by the embodiment of the invention has at least the following beneficial effects:
through setting up a plurality of conveying channels, form multiple different flow paths, under the control of the pump body, accomplish the preprocessing operation, avoid artificial intervention to the influence of detection accuracy, reduce the technical requirement of detection.
According to other embodiments of the present invention, the gene sequencing device further comprises a first air supply unit, wherein the first air supply unit is communicated with the solution chamber output end and is used for reversely draining liquid to the solution chamber input end. Specifically, the first air supply unit can be communicated with the output end of the solution cavity through the fourth conveying channel, and through the arrangement of the first air supply unit, the sample liquid can be reversely pushed back to the solution cavity through the fourth conveying channel, so that the coverage test can be rapidly and conveniently completed.
According to the gene sequencing device of other embodiments of the present invention, the first air supply unit includes an air pump and a first air supply channel, the first air supply channel communicates the air pump with the fourth conveying channel and forms a first air supply channel with the solution chamber, and air is made to enter the fourth conveying channel from the air pump and further convey the solution from the fourth conveying channel back to the solution chamber through the switch of the air pump, so as to complete back sample injection.
According to the gene sequencing device of other embodiments of the present invention, a filter screen is further disposed in the first air supply channel, and through the arrangement of the filter screen, the purity of the air supply is ensured, and the influence of external pollution sources on the detection accuracy is avoided.
According to other embodiments of the present invention, the gene sequencing apparatus further comprises a second air supply unit, which is in communication with the solution chamber input. Through the setting of the second air supply unit, the tested chip can be blow-dried after solvent feeding is finished in the sequencing process and before the tested chip is taken out after testing is finished, so that the detection accuracy is ensured.
According to other embodiments of the present invention, the second air supply unit includes a second air supply channel. The second air supply channel, the solution cavity, the fourth conveying channel and the pump body form a second air supply channel, and after the second air supply channel and the pump body are opened, external air is supplied into the solution cavity to blow-dry the second air supply channel and the pump body, so that the detection accuracy is ensured.
According to other embodiments of the present invention, the genetic sequencing device further comprises a sample injection unit, wherein the sample injection unit comprises a sample injection port and a driving mechanism, and the driving mechanism drives the sample injection port to adjust the distance between the sample injection port and the sample injection port, so that the sample injection port approaches to or is far from the sample injection port.
According to the gene sequencing device of other embodiments of the invention, the driving mechanism comprises a movable seat, the sample injection port is arranged on the movable seat, and the movable seat drives the sample injection port to move, so that the automation degree of sequencing is improved.
According to other embodiments of the present invention, the drive mechanism further comprises: the device comprises a support frame, a slide rail, a motor and a screw rod, wherein the slide rail is arranged on the support frame, and a rotating shaft of the motor is connected with the screw rod.
According to other embodiments of the invention, a side surface of the movable seat, which is close to the sliding rail, is provided with a sliding rail guide block, one end of the movable seat, which is close to the sliding rail, is provided with a through hole, and the through hole is provided with internal threads and is in threaded connection with the screw rod.
According to other embodiments of the present invention, the genetic sequencing device further comprises a bonding seat and an elastic assembly, wherein the bonding seat is elastically connected with the movable seat through the elastic assembly arranged between the bonding seat and the movable seat, and the elastic assemblies are distributed along the circumferential direction of the bonding seat.
According to other embodiments of the present invention, the electric motor is a stepper motor.
The gene sequencing device according to other embodiments of the present invention further includes a heater for maintaining the chip under test at a set temperature. The set temperature refers to a specific temperature required by normal sequencing reaction in a solution cavity of a chip to be tested, and the stable running of the sequencing reaction is ensured by controlling the temperature.
According to the gene sequencing device of other embodiments of the invention, the heater is arranged below the fixed unit, can be fully contacted with the chip to be tested, effectively maintains the reaction temperature, and ensures efficient implementation of the sequencing reaction.
The gene sequencing device according to other embodiments of the present invention further comprises an identification unit for identifying the reagent and/or the chip under test.
According to the gene sequencing device of other embodiments of the present invention, the identification unit is a code scanning module, specifically may be a code scanning module capable of identifying two-dimensional codes or bar codes, and by identifying the identification code on the reagent and/or the tested chip, the identification code is further transmitted to the main board control unit, so as to ensure the normal running of the whole sequencing flow, and avoid errors in the manual reading process.
According to other embodiments of the present invention, the identification unit may be a USB or serial interface, and is disposed on an outer surface of the gene sequencing device.
According to other embodiments of the present invention, a storage unit includes a wash solution storage unit, a buffer storage unit, and a detection reagent storage unit.
According to other embodiments of the present invention, the sample introduction unit is provided with a plurality of groups.
According to other embodiments of the present invention, the genetic sequencing device further includes a touch panel for displaying various information or states during use or receiving a touch command to perform a corresponding action.
According to other embodiments of the present invention, the touch panel is a capacitive or resistive single-point or multi-point touch panel, and the size of the touch panel is 4 to 24 inches.
According to other embodiments of the present invention, the gene sequencing device further comprises a mini PC motherboard, wherein the mini PC motherboard communicates with the control motherboard through a USB interface, and performs a sequencing procedure.
According to other embodiments of the present invention, the mini PC motherboard may be a small industrial computer motherboard or a tablet computer motherboard, including a CPU, a memory, a flash memory, interfaces supporting USB3.0 and USB2.0, independent dual-display output, wired network or wireless WIFI, serial port, and the like.
According to other embodiments of the present invention, the control motherboard is a multi-layered circuit board comprising a single chip microcomputer, a temperature sensor, an input/output interface, a chip socket under test, a valve control circuit, a temperature control circuit, a pump control circuit, an electromagnet control circuit, a stepper motor control circuit, a fan/LED control circuit, an amplifier, a buffer, and the like.
According to other embodiments of the present invention, the temperature sensor is used to monitor the temperature inside the sequencing system, ensuring that the temperature of the heater remains normal. The temperature sensor can be arranged on the main board or at any position in the case and is connected with the main board.
According to other embodiments of the present invention, the control main board and the stepper motor are mounted on a stage mounted on a base mounted on a housing.
In a second aspect, an embodiment of the present invention provides a gene sequencing method using the above-described gene sequencing device, including the steps of:
(1) The following tests were performed in order:
and (3) testing the fit degree: the device is used for detecting the fitting degree of the tested chip and the fixing unit;
wet measurement: after the storage unit is rinsed by the buffer solution output by the third conveying channel to the solution cavity, detecting whether the micro holes of the tested chip are filled with the buffer solution or not;
coverage test: the sample injection unit inputs first sample liquid into the solution cavity through the second conveying channel for rinsing, sucks the first sample liquid in the solution cavity to the fourth conveying channel, reversely discharges the first sample liquid back into the solution cavity, and detects whether a sample liquid covering layer is formed on the surface of the micro-hole after repeated for a plurality of times;
and (3) coating thinning test: the storage unit outputs buffer solution to the solution cavity from the third conveying channel so as to thin the sample solution covering layer, and then detection is carried out, wherein the detection is to measure the capacitance values of the two sides of the sample solution covering layer;
electroporation and single well test: after a second sample liquid is input into the solution cavity from the second conveying channel for rinsing, the sample liquid cover layer is detected whether to be electroporated or not and a nanopore is formed;
(2) And after the test is passed, a third sample solution is input into the solution cavity through the second conveying channel by the sample injection unit, and sequencing is started.
According to other embodiments of the present invention, the fitness test is to detect the electrical signal between the stationary unit and the chip under test.
According to the gene sequencing method of other embodiments of the present invention, elastic components (e.g., springs) are disposed at four corners of the fixed unit corresponding to the tested chip, pins are disposed at four positions of the tested chip corresponding to the elastic components, and the pins can be electrically connected with corresponding sockets on the control motherboard to transmit electrical signals to the control motherboard. When the pressing force of the elastic component reaches a preset value, namely the preset fitting degree is achieved between the tested chip and the control main board, the tested chip transmits an electric signal to the control board, and the control main board can read the electric signal normally when detecting the electric signal; if the electric signal cannot be read normally, the problem of the bonding degree between the tested chip and the fixed unit is indicated.
According to other embodiments of the present invention, the method for gene sequencing comprises thinning the sample fluid cover layer including the first cover layer thinning, the second cover layer thinning, the third cover layer thinning, and the fourth cover layer thinning, and detecting the capacitance value between both sides of the sample fluid cover layer is performed after the fourth cover layer thinning. Wherein the first cover layer thinning, the second cover layer thinning, the third cover layer thinning and the fourth cover layer thinning differ in that the input speeds of the buffers are different.
Drawings
FIG. 1 is a schematic diagram showing the general structure of a gene sequencing device according to a first embodiment;
FIG. 2 is a partial schematic view of the gene sequencing device of FIG. 1;
FIGS. 3 to 7 are flowcharts of gene sequencing using the gene sequencing device of FIG. 1;
FIG. 8 is a schematic diagram showing the general structure of a gene sequencing device according to the second embodiment.
Detailed Description
The conception and the technical effects produced by the present invention will be clearly and completely described in conjunction with the embodiments below to fully understand the objects, features and effects of the present invention. It is apparent that the described embodiments are only some embodiments of the present invention, but not all embodiments, and that other embodiments obtained by those skilled in the art without inventive effort are within the scope of the present invention based on the embodiments of the present invention.
In the description of the embodiments of the present invention, if an orientation description such as "upper", "lower", "front", "rear", "left", "right", etc. is referred to, it is merely for convenience of description and simplification of the description, and it is not indicated or implied that the apparatus or component referred to must have a specific orientation, be configured and operated in a specific orientation, and thus should not be construed as limiting the present invention.
In the description of the embodiments of the present invention, if a feature is referred to as being "disposed", "fixed", "connected" or "mounted" on another feature, it can be directly disposed, fixed or connected to the other feature or be indirectly disposed, fixed or connected or mounted on the other feature. In the description of the embodiments of the present invention, if "several" is referred to, it means more than one, if "multiple" is referred to, it is understood that the number is not included if "greater than", "less than", "exceeding", and it is understood that the number is included if "above", "below", "within" is referred to. If reference is made to "first", "second" it is to be understood as being used for distinguishing technical features and not as indicating or implying relative importance or implicitly indicating the number of technical features indicated or implicitly indicating the precedence of the technical features indicated.
Example 1
Referring to fig. 1 and 2, there is provided a gene sequencing device including a base 100, a control main board 200, a temperature sensor 210, a code scanning module 220, an electromagnet 230, a heater 250, a stepping motor 240, a reagent bottle 310, a valve 320, a pump 330, a waste bottle 350, a fan/LED 260. A chip under test 340 for testing is also mounted on the control motherboard 200.
The base 100 is used to carry the components of the gene sequencing device, including the control motherboard 200, valves 320, pumps 330, and fans/LEDs 260, among others. The control main board 200 and the stepping motor 240 are mounted on a table (not shown in the drawing), the table is mounted on the base 100, and the base 100 is mounted on a housing (not shown in the drawing).
The control main board 200 receives a sequencing program command running on the mini PC main board 410 for controlling the actual operation of the gene sequencing device; the control motherboard 200 is a multi-layered circuit board and comprises a single chip microcomputer, an input/output interface 600, a chip socket under test 700, a valve control circuit 201, a temperature control circuit 202, a pump control circuit, an electromagnet control circuit, a stepper motor control circuit, a fan/LED 260 control circuit, an amplifier, a buffer, and the like. The tested chip socket 700 is provided with probes 710 for connecting the tested chip 340 and the control motherboard 200 and transmitting electrical signals for testing the fit. The driving mechanism 800 is driven by the stepper motor 240 to control the movement of the liquid path and the electromagnet module 230 in the gene sequencing device, and fix and closely attach the chip under test 340 and the control motherboard 200.
The temperature sensor 210 is installed on the control main board 200 for monitoring the ambient temperature inside the gene sequencing device.
The code scanning module 220 is mounted on the housing, and is electrically connected to the control main board 200, and is used for scanning bar codes on the tested chip 340 and various reagent bottles 310, and transmitting the obtained bar codes to the control main board 200.
The electromagnet module 230 is mounted on the driving mechanism 800, and is used for controlling the switch of the microfluidic valve on the tested chip 340.
The heater 250 is installed below the chip under test socket 700 for heating the chip under test 340 to maintain a stable temperature of the chip under test 340 during the test.
The stepper motor 240 is mounted on the workbench and used for driving the driving mechanism 800 to achieve the purposes of automatically positioning and tightly attaching the tested chip 340 and the control motherboard 200.
A reagent bottle 310 is mounted on the housing for filling with a reagent for detection.
A valve 320 is mounted on the base 100 for controlling the opening and closing or flow direction of the fluid path.
A pump 330 is installed on the base 100 for sucking or discharging a reagent or air.
The test chip 340 is a disposable material for detecting a gene sample or a specimen.
The waste bottle 350 is mounted on the housing for filling waste discharged after the test, and is disposable, and the waste bottle 350 is discarded after being filled.
A fan/LED 260 is secured to the housing, wherein the fan is used to assist in heat dissipation from the machine and the LED is used to indicate the machine's operational status.
The control motherboard 200 of the gene sequencing device is connected with the mini PC motherboard 410 through the USB2.0 and USB3.0 interfaces, and the mini PC motherboard 410 is connected with the external router 412 through the LAN network interface through the keyboard/mouse 413 externally connected with the USB2.0 interface and the display 411 externally connected with the HDMI interface.
Referring to fig. 2, the internal concrete structure of the gene sequencing device is shown, which mainly includes a storage unit, a first transfer channel 510, a sample introduction unit, a solution chamber input 531, a solution chamber output 532, a second transfer channel 520, a third transfer channel 530, a fourth transfer channel 540, a fifth transfer channel 550, a fixing unit, a first pump body 331-1, a second pump body 331-2, an air pump 332, and the like.
The storage unit includes a plurality of reagent bottles 310, and the reagent bottles 310 can be specifically divided into an isopropanol reagent bottle 311, a buffer reagent bottle 312, a deionized water reagent bottle 313, a first reagent bottle 314, and a second reagent bottle 315, where each reagent bottle 310 is opened and closed by a reagent bottle control valve 321. The output pipeline of the reagent bottle 310 is connected in parallel to a first VIN381, the first VIN381 is respectively connected with a first conveying channel control valve 323 and a third conveying channel control valve 322, the first conveying channel control valve 323 controls the opening and closing of a first conveying channel 510 connected with the first VIN381, the first conveying channel 510 is connected with a fourth conveying channel 540 and is communicated with a waste liquid bottle 350 through a VOUT390, and the waste liquid bottle 350 is also connected with a pump body 331 through a pump body control valve 326.
The third transfer channel control valve 322 controls the opening and closing of the third transfer channel 530 connected to the first VIN381, and the third transfer channel 530 is connected to the second transfer channel 520 at the solution chamber input 531. A solution cavity 341 of the chip 340 under test is arranged between the solution cavity input end 531 and the solution cavity output end 532, the solution cavity output end 532 is connected with the VOUT control valve 325, and the connection and disconnection of the solution cavity and the waste liquid bottle are controlled by the VOUT control valve 325.
The sample introduction unit comprises: the first sample inlet 371 (VS 1) and the first temporary storage chamber 361 connected with the first sample inlet 371, the second sample inlet 372 (VS 2) and the second temporary storage chamber 362 connected with the second sample inlet 372, the third sample inlet 373 (VS 3) and the third temporary storage chamber 363 connected with the third sample inlet. The first temporary storage chamber 361, the second temporary storage chamber 362 and the third temporary storage chamber 363 are respectively connected with a first temporary storage chamber control valve 324-1, a second temporary storage chamber control valve 324-2 and a third temporary storage chamber control valve 324-3, and are connected in parallel and then connected with the solution chamber input end 531 through a second conveying channel 520.
The first temporary storage chamber control valve 324-1, the second temporary storage chamber control valve 324-2, the third temporary storage chamber control valve 324-3, the third conveying channel control valve 322, the first conveying channel control valve 323 and the VOUT control valve 325 in the valve 320 are respectively controlled to open and close by a first electromagnet 231, a second electromagnet 232, a third electromagnet 233, a fourth electromagnet 234, a fifth electromagnet 235 and a sixth electromagnet 236 in the electromagnet module 230, and the first electromagnet 231, the second electromagnet 232, the third electromagnet 233, the fourth electromagnet 234, the fifth electromagnet 235 and the sixth electromagnet 236 are connected with the valve control circuit 201 and controlled by the control main board 200.
The first air supply unit includes an air pump 332, and the air pump 332 is connected with two parallel liquid supply valves 327-1 and 327-2, and a third reagent bottle 911 and a first filter 912 are sequentially disposed at the front end. The gas feed valve 327-1 and the first gas feed valve 327-2 are connected to the second VIN382 via a first gas feed channel. The second VIN382 is coupled to the fourth delivery channel 540 via the fifth delivery channel 550 and further coupled to the solution chamber output 532. The air pump 332 is also connected to the control main board 200.
The second air supply unit includes a second air supply channel, an inlet of which is provided with a second filter 913, and the opening and closing of which is controlled by a second air supply valve 328, and the second air supply channel is connected to the first conveying channel 510 and the third conveying channel 530 through the first VIN 381.
The first pump body 331-1 and the second pump body 331-2 are negative pressure pumps, the first pump body 331-1 is controlled to be communicated with the waste liquid bottle 350 by the first pump body control valve 326-1, the second pump body 331-2 is controlled to be communicated with the waste liquid bottle 350 by the second pump body control valve 326-2, and the first pump body 331-1 and the second pump body 331-2 can control liquid in a liquid path of the gene sequencing device to achieve different liquid flow rates.
The sequencing method of the gene sequencing device refers to fig. 3-7, and is combined with fig. 2, and specifically comprises the following steps:
referring to fig. 3, when the user operates the genetic sequencing device to perform sequencing, if the system is not yet started, the power supply is turned on first to start the device, and when the system is started, the LED initialization, the fan initialization, the heater initialization, the stepper motor initialization, the pump initialization, the valve initialization, the code scanning module initialization and the temperature sensor initialization are automatically performed first, and then the computer is waited for connecting to perform further actions.
After the computer is connected with the machine, if a new reagent bottle needs to be installed, firstly, the bar code on the reagent bottle is scanned by a code scanning module and then is installed on the corresponding reagent bottle position; and then the bar code on the tested chip is scanned by the code scanning module and then is placed on a tested chip socket, then a command is given to a computer through a keyboard/mouse to execute a sequencing program, after the sequencing is finished, the computer displays a sequencing result through a display, a sequencing report is stored in a computer hard disk, and if necessary, the sequencing report can be uploaded to a cloud for centralized management or sharing with other people through a router. And after the sequencing is finished, taking out the tested chip from the gene sequencing device, if the sequencing is still to be continued, putting a new tested chip on the gene sequencing device again, otherwise, exiting the sequencing program, and closing the machine to finish the operation.
Referring to fig. 4, when the sequencing program starts, the stepper motor is first zeroed, i.e., moved to a starting position, which is determined by the position of the position sensor. After the stepping motor is reset to the zero position, the sequencing program controls the stepping motor to move downwards by a larger extent (about 8000-10500 steps), then the tightness test of the tested chip and the control main board is carried out, the tightness test is determined by reading the related parameters of the tested chip, if the read parameters are normal, the tested chip passes the tightness test with the control main board, if the stepping motor reaches the maximum limit and does not pass the tightness test yet, the tested chip is judged to be a defective product, the sequencing process controls the stepping motor to be reset to the zero position, and then the sequencing work is finished; if the stepper motor has not reached the maximum limit, the sequencing program will control the stepper motor to move slightly further down and then continue to read the relevant parameters on the chip under test for further compactness testing.
The specific detection of the parameters may be as follows:
elastic components (such as springs) are arranged at four corners of the tested chip, pins are arranged at four positions of the tested chip corresponding to the elastic components, and can be electrically connected with corresponding sockets on the control main board to transmit electric signals to the control main board. When the pressing force of the elastic component reaches a preset value, namely the preset fitting degree is achieved between the tested chip and the control main board, the tested chip transmits an electric signal to the control board, and the control main board can read the electric signal normally.
After the tested chip passes the tightness test, the sequencing process controls to close the pump body, the air pump, the valve and the electromagnet, firstly, an isopropyl alcohol (IPA) cleaning system is utilized, and isopropyl alcohol reagent is discharged into a waste liquid bottle from an isopropyl alcohol reagent bottle through a reagent bottle control valve, a first VIN, a first conveying channel control Valve (VBPY), a first conveying channel, a fourth conveying channel and VOUT, so that the cleaning system is completed; after the cleaning is completed, the pump body and the reagent bottle control valve for controlling the isopropanol reagent bottle are turned off. Then using deionized water (DIWATER) to clean the system, and discharging deionized water from the deionized water reagent bottle to the waste liquid bottle through the reagent bottle control valve, the first VIN, the first conveying channel control valve, the first conveying channel, the fourth conveying channel and VOUT to complete the cleaning of the system; after the cleaning is finished, the pump body and a reagent bottle control valve for controlling the deionized water reagent bottle are turned off, and then an electromagnet for controlling the first conveying channel control valve is turned off. If the sequencing of a plurality of chips under test is performed continuously, the system cleaning operation is performed once.
After the system cleaning work is finished, the sequencing program firstly carries out wet testing on the tested chip, and before the wet testing is carried out, the pre-cleaning work is carried out on the tested chip so as to drain the air in the tested chip and the liquid path, a pump body and corresponding valves are opened to enable buffer solution to enter a solution cavity of the tested chip from a buffer solution reagent bottle through corresponding reagent bottle control valves, a first VIN, a third conveying access control valve, a third conveying channel and a solution cavity input end, and then the buffer solution is discharged into a waste liquid bottle through a solution cavity output end, a VOUT control valve, a fourth conveying channel and a VOUT so as to clean the tested chip; after the chip to be tested is cleaned, the pump body and a reagent bottle control valve for controlling a buffer reagent bottle are turned off, then an electromagnet for controlling a second conveying channel control valve of VIN and an electromagnet for controlling a VOUT control valve of VOUT are turned off, and then a sequencing program executes the wet testing. If the wet test is passed, the system is dried, otherwise, buffer solution is continuously added, and the pre-cleaning operation is performed once again until the wet test is passed.
Referring to fig. 5, before solvent is fed into the chip to be tested through wet measurement, the chip to be tested needs to be dried first, a second air feeding valve is opened, dry air is fed through a second air feeding unit, and then enters a solution cavity of the chip to be tested through a second filter screen, the second air feeding valve, the first VIN, a third conveying channel control valve, a third conveying channel and a solution cavity input end, and is discharged into a waste liquid bottle through the solution cavity output end, the VOUT control valve, a fourth conveying channel and VOUT; after the tested chips are dried, the pump body, the second air supply valve, the electromagnet for controlling the third conveying channel valve of the first VIN and the electromagnet for controlling the VOUT control valve of the VOUT are turned off.
When solvent is to be fed, firstly, a reagent bottle control valve is opened, a related valve and a pump body are opened, so that the solvent enters a solution cavity of a tested chip from a reagent bottle through the reagent bottle control valve, a first VIN, a third conveying channel control valve, a third conveying channel and a solution cavity input end, and is discharged into a waste liquid bottle through a solution cavity output end, a VOUT control valve, a fourth conveying channel and a VOUT; after the solvent is fed, the pump body and the reagent bottle control valve are closed, and then the electromagnet of the third conveying channel control valve for controlling the first VIN and the electromagnet of the VOUT control valve for controlling the VOUT are closed.
After solvent is fed, the tested chip is required to be dried firstly to feed a sample of the sample, corresponding valves are opened, dry air enters a solution cavity of the tested chip through a second filter screen, a second air feeding valve, a first VIN, a third conveying channel control valve, a third conveying channel and a solution cavity input end, and then is discharged into a waste liquid bottle through a solution cavity output end, a VOUT control valve, a fourth conveying channel and a VOUT; after the tested chips are dried, the pump body, the second air supply valve, the electromagnet of the third conveying channel control valve for controlling the first VIN and the electromagnet of the VOUT control valve for controlling the VOUT are turned off.
Before a sample is fed, the electromagnet of the temporary storage cavity control valve corresponding to the sample inlet is closed, then the sample 1 is added from the first sample inlet to the first temporary storage cavity, the sample 2 is added from the second sample inlet to the second temporary storage cavity, the sample 3 is added from the third sample inlet to the third temporary storage cavity, then the electromagnet of the third temporary storage cavity control valve controlling the third sample inlet and the electromagnet of the VOUT control valve controlling VOUT are opened, the pump body is opened to suck the sample 3 from the third temporary storage cavity, the sample is fed into the solution cavity of the tested chip through the temporary storage cavity control valve, the second conveying channel and the solution cavity input end, and then the electromagnet of the third temporary storage cavity control valve controlling the third sample inlet, the electromagnet of the VOUT control valve controlling the VOUT and the pump body are turned off so as to execute coverage test (COVERTEST). If the coverage test is not passed, adding the sample 3 from the third sample inlet to the third temporary storage cavity once, and repeating the sample feeding operation so as to perform the sample feeding back and forth.
If the coverage test fails, besides adding the sample 3 again from the third sample inlet, the sample 3 can also be stored in a third reagent bottle, and the sample 3 is pumped into a fifth conveying channel from the second VIN when needed, and enters the solution cavity through the fourth conveying channel and the output end of the solution cavity.
When in reverse sampling, the first air feeding valve is firstly opened, then the air pump and the electromagnet of the third temporary storage cavity control valve for controlling the third sample inlet are opened, at the moment, after the dry air comes out of the air pump, the sample 3 is reversely pushed back into the solution cavity from the fourth conveying channel and the output end of the solution cavity through the first filter screen, the first air feeding valve, the first air feeding channel, the second VIN and the fifth conveying channel, and finally, the electromagnet, the first air feeding valve and the air pump of the third temporary storage cavity control valve for controlling the third sample inlet are closed, so that the reverse sampling action is completed. And then, executing forward sample injection operation once, performing coverage test after executing reverse and forward sample injection operation for a plurality of times, if the sample injection operation does not pass, indicating that the tested chip is defective, controlling the stepping motor to return to zero position in the sequencing process, and ending the sequencing work.
Referring to fig. 6, after the chip under test passes the coverage test, the sequencing program first cleans the fluid path and then continues the THINNING1 (thin 1) test. Firstly, controlling to open a reagent bottle control valve for storing a buffer reagent bottle and an electromagnet for controlling a first conveying channel control valve by a sequencing program, and then opening a pump body to enable the buffer to be discharged from the buffer reagent bottle to a waste liquid bottle through the reagent bottle control valve, a first VIN, the first conveying channel control valve, a first conveying channel, a fourth conveying channel and a VOUT so as to finish the work of a cleaning liquid path; then turning off the electromagnet controlling the first conveying channel control valve, turning on the electromagnet controlling the third conveying channel control valve of the first VIN and the electromagnet controlling the VOUT control valve of the VOUT, so that the buffer solution reagent enters the solution cavity of the tested chip from the buffer solution reagent bottle through the reagent bottle control valve, the first VIN, the third conveying channel control valve, the third conveying channel and the solution cavity input end, and then is discharged into the waste liquid bottle through the solution cavity output end, the VOUT control valve, the fourth conveying channel and the VOUT so as to perform a first-stage cover layer THINNING action (THINNING 1) on the tested chip; after the first stage thinning of the tested chip is completed, the pump body and a reagent bottle control valve for controlling a buffer reagent bottle are turned off, then an electromagnet for controlling a third conveying channel control valve of the first VIN and an electromagnet for controlling a VOUT control valve of the VOUT are turned off, and then the thinning1 test is executed by a sequencing program.
After the thinning1 test is finished, continuing to perform second, third and fourth-stage cover layer thinning actions, opening an electromagnet of a third conveying channel control valve for controlling a first VIN and an electromagnet of a VOUT control valve for controlling VOUT by a sequencing program, opening a pump body and a reagent bottle control valve for controlling a buffer reagent bottle to perform the second-stage cover layer thinning action (T2), changing the flow rate after a period of time to continue the third-stage cover layer thinning action (T3), changing the flow rate after a period of time to continue the fourth-stage cover layer thinning action (T4), then closing the pump body and the reagent bottle control valve for controlling the buffer reagent bottle, closing the electromagnet of the third conveying channel control valve for controlling the first VIN and the electromagnet of the VOUT control valve for controlling VOUT, and executing the thinning 4 test by the sequencing program.
If the test passes the thinning 4 test, the action of the sample 1 is continued, otherwise, the second, third and fourth-stage cover layer thinning actions are continued, the thinning 4 test is tested for one time, if the thinning 4 test is continuously repeated for 20 times and still not passed, the tested chip is judged to be defective, the sequencing process controls the stepping motor to return to zero, and then the sequencing work is ended.
After the test of thinning 4, the sequencing program opens the electromagnet of the first temporary storage cavity control valve for controlling the first sample inlet and the electromagnet of the VOUT control valve for controlling VOUT, opens the pump body to suck the sample 1 out of the first temporary storage cavity, enters the solution cavity of the tested chip through the temporary storage cavity control valve, the second input channel and the solution cavity input end, and then turns off the electromagnet of the first temporary storage cavity control valve for controlling the first sample inlet, the electromagnet of the VOUT control valve for controlling VOUT and the pump body so as to execute electroporation and single-hole test.
After electroporation and single well testing, the sample 2 is injected with the buffer solution again; the sequencing program firstly opens the electromagnet of the third conveying channel control valve for controlling the first VIN and the electromagnet of the VOUT control valve for controlling the VOUT, then opens the pump body and the reagent bottle control valve for controlling the buffer reagent bottle, so that the buffer reagent enters the solution cavity of the tested chip from the buffer reagent bottle through the reagent bottle control valve, the first VIN, the third conveying channel control valve, the third conveying channel and the solution cavity input end, then closes the pump body and the reagent bottle control valve for controlling the buffer reagent bottle, then closes the electromagnet of the third conveying channel control valve for controlling the first VIN and the electromagnet of the VOUT control valve for controlling the VOUT control valve, then the sequencing program opens the electromagnet of the second temporary storage cavity control valve for controlling the second sample inlet and the electromagnet of the VOUT control valve, and opens the pump body to suck the sample 2 from the second temporary storage cavity, and then enters the solution cavity of the tested chip through the temporary storage cavity control valve, the second conveying channel and the solution cavity input end, and then closes the electromagnet of the second temporary storage cavity control valve for controlling the second sample inlet and the electromagnet of the VOUT control valve for controlling the sequencing so as to execute gene work.
Referring to FIG. 7, after the gene sequencing process is completed, the sequencing program is to clean the chip and the liquid path to be tested by using buffer, isopropanol and deionized water; when the buffer is used for cleaning, an electromagnet of a third conveying channel control valve for controlling the first VIN and an electromagnet of a VOUT control valve for controlling the VOUT are opened, and then a pump body and a reagent bottle control valve for controlling a buffer reagent bottle are opened, so that buffer reagent enters a solution cavity of a tested chip from the reagent bottle through the reagent bottle control valve, the first VIN, the third conveying channel control valve, the third conveying channel and the solution cavity input end, and is discharged into a waste liquid bottle through the solution cavity output end, the VOUT control valve, the fourth conveying channel and the VOUT; then the pump body and a reagent bottle control valve for controlling the buffer reagent bottle are turned off, and then the electromagnet of the third conveying channel control valve for controlling the first VIN and the electromagnet of the VOUT control valve for controlling the VOUT are turned off.
When the device is cleaned by isopropanol, an electromagnet of a third conveying channel control valve for controlling the first VIN and an electromagnet of a VOUT control valve for controlling the VOUT are opened, and then a pump body and a reagent bottle control valve for controlling an isopropanol reagent bottle are opened, so that isopropanol reagent enters a solution cavity of a tested chip from the isopropanol reagent bottle through the reagent bottle control valve, the first VIN, the third conveying channel control valve, the third conveying channel and the solution cavity input end, and is discharged into a waste liquid bottle through the solution cavity output end, the VOUT control valve, the fourth conveying channel and the VOUT; then the pump body and the reagent bottle control valve for controlling the isopropanol reagent bottle are turned off, and then the electromagnet for controlling the third conveying channel control valve of the first VIN and the electromagnet for controlling the VOUT control valve of the VOUT are turned off.
When the deionized water is used for cleaning, an electromagnet of a third conveying channel control valve for controlling the first VIN and an electromagnet of a VOUT control valve for controlling the VOUT are opened, and then a pump body and a reagent bottle control valve for controlling a deionized water reagent bottle are opened, so that deionized water reagent enters a solution cavity of a tested chip from the deionized water reagent bottle through the reagent bottle control valve, the first VIN, the third conveying channel control valve, the third conveying channel and the solution cavity input end, and is discharged into a waste liquid bottle through the solution cavity output end, the VOUT control valve, the fourth conveying channel and the VOUT; then the pump body and a reagent bottle control valve for controlling the deionized water reagent bottle are turned off, and then an electromagnet for controlling a third conveying channel control valve of the first VIN and an electromagnet for controlling a VOUT control valve of the VOUT are turned off.
Before the tested chip is taken out after the test is finished, the tested chip is also required to be dried; at the moment, the sequencing program firstly opens a second air supply valve, then opens an electromagnet of a third conveying channel control valve for controlling the first VIN and an electromagnet of a VOUT control valve for controlling the VOUT, and then opens a pump body, so that dry air enters a solution cavity of a tested chip through a second filter screen, the second air supply valve, the first VIN, the third conveying channel control valve, a third conveying channel and a solution cavity input end, and is discharged into a waste liquid bottle through the solution cavity output end, the VOUT control valve, a fourth conveying channel and the VOUT; after the tested chip is dried, the pump body, the second air supply valve, the electromagnet of the third conveying channel control valve for controlling the first VIN and the electromagnet of the VOUT control valve for controlling the VOUT are turned off; and finally controlling the stepping motor to return to the part, and ending the sequencing work.
Example 2
Referring to fig. 8, another gene sequencing device is shown, which includes a base 100, a control motherboard 200, a temperature sensor 210, a code scanning module 220, an electromagnet module 230, a heater 250, a stepper motor 240, a reagent bottle 310, a valve 320, a pump 330, a waste bottle 350, a fan/LED 260. The control main board 200 of the gene sequencing device is connected with a personal computer 420 through a USB2.0 and USB3.0 interface, and the personal computer 420 is connected with an external router 412 through a LAN network interface by a keyboard/mouse 413 externally connected with the USB2.0 interface and a display 411 externally connected with an HDMI interface.
The embodiments of the present invention have been described in detail with reference to the accompanying drawings, but the present invention is not limited to the above embodiments, and various changes can be made within the knowledge of one of ordinary skill in the art without departing from the spirit of the present invention. Furthermore, embodiments of the invention and features of the embodiments may be combined with each other without conflict.
Claims (9)
1. A genetic sequencing device comprising:
a storage unit for storing a reagent;
the first conveying channel is connected with the storage unit and the waste liquid bottle to form a straight-through flow path;
the solution cavity input end is used for communicating a solution cavity of the chip to be tested and feeding solution into the solution cavity;
the solution cavity output end is used for being communicated with the solution cavity of the chip to be tested and discharging liquid from the solution cavity;
the sample injection unit comprises a sample injection port and a temporary storage cavity which are sequentially connected;
the second conveying channel is connected with the temporary storage cavity and the input end of the solution cavity;
the third conveying channel is connected with the storage unit and the input end of the solution cavity;
the fourth conveying channel is connected with the waste liquid bottle and the output end of the solution cavity;
the fixing unit is used for fixing the tested chip;
the pump body is used for enabling the reagent and the sample liquid to flow through the solution cavity to react;
the gene sequencing device further comprises a first air supply unit, wherein the first air supply unit comprises an air pump and a first air supply channel, and the first air supply channel is used for communicating the air pump with the fourth conveying channel and forming a first air supply channel with the solution cavity, so that the sample liquid is reversely pushed back to the solution cavity by the fourth conveying channel.
2. The genetic sequencing apparatus of claim 1, further comprising a second air delivery unit in communication with the solution chamber input.
3. The genetic sequencing apparatus of claim 1, further comprising a sample injection unit comprising a sample injection port and a drive mechanism that drives the sample injection port to adjust a distance from the sample injection port.
4. A gene sequencing device according to any one of claims 1 to 3, further comprising a heater for maintaining the chip under test at a set temperature.
5. A genetic sequencing device according to any one of claims 1 to 3, further comprising an identification unit for identifying the reagent and/or the chip under test.
6. The genetic sequencing apparatus according to any one of claims 1 to 3, wherein the storage unit comprises a washing liquid storage unit, a buffer storage unit, and a detection reagent storage unit.
7. A method of gene sequencing using the gene sequencing device of any one of claims 1 to 6, comprising the steps of:
(1) The following tests were performed in order:
and (3) testing the fit degree: the device is used for detecting the fitting degree of the tested chip and the fixing unit;
wet measurement: after the storage unit is rinsed by the buffer solution output by the third conveying channel to the solution cavity, detecting whether the micro holes of the tested chip are filled with the buffer solution or not;
coverage test: the sample injection unit inputs first sample liquid into the solution cavity through the second conveying channel for rinsing, sucks the first sample liquid in the solution cavity to the fourth conveying channel, reversely discharges the first sample liquid back into the solution cavity, and repeatedly detects whether a sample liquid covering layer is formed on the surface of the micro-hole or not;
and (3) coating thinning test: the storage unit outputs buffer solution to the solution cavity through the third conveying channel so as to thin the sample solution covering layer, and then detection is carried out, wherein the detection is used for measuring capacitance values of two sides of the sample solution covering layer;
electroporation and single well test: after a second sample liquid is input into the solution cavity from the second conveying channel for rinsing, the sample liquid cover layer is detected whether to be electroporated or not and a nanopore is formed;
(2) And after the test is passed, a third sample solution is input into the solution cavity through the second conveying channel by the sample injection unit, and sequencing is started.
8. The method of claim 7, wherein the fitness test is to detect an electrical signal between the immobilization unit and the chip under test.
9. The method of claim 7, wherein said thinning of said sample fluid cover layer comprises a first cover layer thinning, a second cover layer thinning, a third cover layer thinning, and a fourth cover layer thinning, said detecting being performed after said fourth cover layer thinning.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911231595.0A CN111040942B (en) | 2019-12-05 | 2019-12-05 | Gene sequencing device and gene sequencing method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911231595.0A CN111040942B (en) | 2019-12-05 | 2019-12-05 | Gene sequencing device and gene sequencing method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN111040942A CN111040942A (en) | 2020-04-21 |
| CN111040942B true CN111040942B (en) | 2023-06-27 |
Family
ID=70234643
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201911231595.0A Active CN111040942B (en) | 2019-12-05 | 2019-12-05 | Gene sequencing device and gene sequencing method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN111040942B (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114250144A (en) * | 2021-12-17 | 2022-03-29 | 深圳铭毅智造科技有限公司 | Liquid path system for gene sequencing |
| CN114410459A (en) * | 2022-01-11 | 2022-04-29 | 深圳清华大学研究院 | Gene sequencing device and sequencing method |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1408884A (en) * | 2001-09-22 | 2003-04-09 | 香港基因晶片开发有限公司 | Device and method for detecting gene sequence |
| CN105199949A (en) * | 2015-09-15 | 2015-12-30 | 深圳市瀚海基因生物科技有限公司 | Fluid control device of gene sequencing |
| CN105733936A (en) * | 2014-12-12 | 2016-07-06 | 深圳华大基因研究院 | Gene sequencing instrument |
| WO2017028760A1 (en) * | 2015-08-14 | 2017-02-23 | 深圳市瀚海基因生物科技有限公司 | Single molecule sequencing method, device, system, and application |
| CN108642159A (en) * | 2018-06-27 | 2018-10-12 | 上海百傲科技股份有限公司 | Biological chips detection system |
| CN109370890A (en) * | 2018-10-25 | 2019-02-22 | 广州市金圻睿生物科技有限责任公司 | Gene sequencer, liquid channel system and its automatic testing method |
| CN109852679A (en) * | 2019-03-01 | 2019-06-07 | 赛纳生物科技(北京)有限公司 | A kind of gene sequencing chip recognition methods |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20120077260A1 (en) * | 2009-03-30 | 2012-03-29 | Fraunhofer Usa, Inc. | Reservoir-buffered mixers and remote valve switching for microfluidic devices |
-
2019
- 2019-12-05 CN CN201911231595.0A patent/CN111040942B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1408884A (en) * | 2001-09-22 | 2003-04-09 | 香港基因晶片开发有限公司 | Device and method for detecting gene sequence |
| CN105733936A (en) * | 2014-12-12 | 2016-07-06 | 深圳华大基因研究院 | Gene sequencing instrument |
| WO2017028760A1 (en) * | 2015-08-14 | 2017-02-23 | 深圳市瀚海基因生物科技有限公司 | Single molecule sequencing method, device, system, and application |
| CN105199949A (en) * | 2015-09-15 | 2015-12-30 | 深圳市瀚海基因生物科技有限公司 | Fluid control device of gene sequencing |
| CN108642159A (en) * | 2018-06-27 | 2018-10-12 | 上海百傲科技股份有限公司 | Biological chips detection system |
| CN109370890A (en) * | 2018-10-25 | 2019-02-22 | 广州市金圻睿生物科技有限责任公司 | Gene sequencer, liquid channel system and its automatic testing method |
| CN109852679A (en) * | 2019-03-01 | 2019-06-07 | 赛纳生物科技(北京)有限公司 | A kind of gene sequencing chip recognition methods |
Also Published As
| Publication number | Publication date |
|---|---|
| CN111040942A (en) | 2020-04-21 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN111040942B (en) | Gene sequencing device and gene sequencing method | |
| US6183693B1 (en) | Random access slide stainer with independent slide heating regulation | |
| WO2017210976A1 (en) | Urine analysis system and urine analysis method thereof | |
| US20220276272A1 (en) | Testing system and method of starting testing system | |
| JP6952708B2 (en) | Self-contained slide processing unit for biological samples | |
| CN109724645A (en) | A kind of portable electric nose system of real-time monitoring gold ear fermentation process state | |
| JP3933499B2 (en) | Sample analyzer and sample analysis system, and containers and racks used in them. | |
| CN106914462A (en) | Capillary viscosimeter automatic flushing device | |
| CN202110183U (en) | Full-automatic plate-washing and incubation integrated machine | |
| WO2017091006A1 (en) | Portable blood viscosity measuring device | |
| CN114354715B (en) | Micro-fluidic chip of blood gas analyzer and matching device and using method thereof | |
| CN203595719U (en) | Automatic analysis device and reaction tray thereof | |
| CN105004868B (en) | A kind of multichannel thrombelastogram instrument and using method | |
| CN110988321A (en) | Detection method and coagulation analysis control method of handheld coagulation analysis system | |
| CN115747031A (en) | Pathogen nucleic acid analysis apparatus | |
| CN115181657A (en) | Device and method for in-situ monitoring of nucleic acid amplification process | |
| CN203117761U (en) | Multiparameter water quality monitoring measurement and control system based on ARM singlechip microcomputer | |
| CN209559896U (en) | A kind of Enzyme-linked washing-board sample-adding all-in-one machine | |
| CN208297438U (en) | A kind of full-automatic permanganate index analysis system | |
| CN207439940U (en) | A kind of multichannel miRNA fast detectors | |
| CN202339347U (en) | Full-automatic ISE (Ion Selective Electrode) biochemical analyzer of integrated electrolyte module | |
| KR101181898B1 (en) | Precision device for detecting bio-materials using the lateral flow immunochromatography test | |
| CN112387432A (en) | Centrifugal time detection mechanism and detection method for high-speed centrifugal equipment | |
| CN217766163U (en) | In-situ solution sample high-throughput screening automatic test system for high-energy light source | |
| CN220019460U (en) | Electronic nose smell analysis system applied to multiple scenes |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |