CN105168325A - Preparation method and application of non-decocted fructus psoraleae preparation - Google Patents
Preparation method and application of non-decocted fructus psoraleae preparation Download PDFInfo
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Abstract
The invention provides a preparation method and an application of a non-decocted fructus psoraleae preparation and belongs to the technical field of traditional Chinese medicine preparation. The preparation method of the non-decocted fructus psoraleae preparation comprises steps as follows: primary extraction of effective ingredients is performed in a mode of combination of water immersion and ultrasonic extraction, secondary extraction is performed through ethanol extraction, and the non-decocted fructus psoraleae preparation is obtained through concentration, drying and superfine grinding finally. The preparation can be used for preparing a compound preparation containing fructus psoraleae or fructus psoraleae extracts. Therefore, the problem that the utilization rate of raw materials is low, the effective ingredients are severely damaged and the extraction efficiency is not high when a conventional single method is adopted for extraction are solved, the whole technological process is simple to control, synergistic interaction of the steps is realized, accordingly, the active ingredients of the fructus psoraleae are effectively protected, and the availability of a finished product is improved better.
Description
Technical field
The invention belongs to Chinese drug preparation technique field, be specifically related to preparation method and application thereof that Fructus Psoraleae exempts from fried dose.
Background technology
Chinese medicine is Chinese nation's crystallization of wisdom, for ensureing that people ' s health has made tremendous contribution.The traditional dosage form of Chinese medicine has pill, powder, unguentum, medicated wine etc., but the deficiencies such as conventional dosage forms majority exists, and taking dose is large, dosage form thick, appearance poor, along with the raising of social development and people's economic level, these deficiencies have seriously constrained the paces of Chinese medicine development, introduce high-tech, utilizing new technique to improve the quality of Chinese medicine, is one of important channel of innovation of Chinese medicinal dosage form.
Fructus Psoraleae is fabaceous dry mature fruit, and the large temperature of its property, acrid in the mouth, hardship, classify spleen, kidney channel, have tonifying YANG, effect of antidiarrheal, is warming the kidney to activate YANG common drug.In addition, Fructus Psoraleae also has estrogen-like effects, coronary artery dilator, promotes the propagation of diploid cell, defying age, improves a series of effects such as the ability of body nonspecific immunity.Containing Multiple components in Fructus Psoraleae, comprise Coumarins, flavonoid and monoterpenes compound etc.
At present, extraction to effective ingredient in Fructus Psoraleae, Fructus Psoraleae are comprised in disease treatment as the preparation etc. of compatibility medical material, Fructus Psoraleae compound preparation to the Land use systems of Fructus Psoraleae.Wherein, mainly concentrate on the extraction to one-components such as psoralen and isopsorapens to the extraction of Fructus Psoraleae effective ingredient, the unification of extract component makes Chinese medicine lose original compound recipe characteristic, limits its application in prescription drug.Next, drinking mostly still based on decoction of formula Chinese medicine.Although in the place of conditions permit, the deposit such as Partial Hospitals, outpatient service has compound decoction finished product, only need heat namely drinkable, but, the curative effect of each secondary compound decoction finished product is fixed, can not dissolve according to the various state of an illness, motility is poor, stores compound decoction finished product simultaneously, existence takes up room, the problem such as waste energy, and compound decoction finished product is in deposit process, and drug effect also can be faded.Moreover Fructus Psoraleae extract and other Chinese crude drug are prepared into compound preparation, and because formula is fixed, curative effect is determined, its range of application receives certain restriction.Therefore, finding efficacy stability, storing simple, that motility is strong Fructus Psoraleae dosage form preparation method is the task of top priority.
Summary of the invention
Technical problem to be solved by this invention is, for the deficiencies in the prior art, provides a kind of efficacy stability, effectively extracts and protects the Fructus Psoraleae of effective ingredient to exempt from the preparation method of fried dose.
For solving the problems of the technologies described above, the technical solution adopted in the present invention is: the preparation method of fried dose exempted from by Fructus Psoraleae, comprises the following steps:
1) corylifolia L is cleaned roguing, less than 60 DEG C dry;
2) get dried corylifolia L and carry out coarse pulverization, cross 20 ~ 40 mesh sieves, obtain coarse powder;
3) through the 2nd) add the water of coarse powder weight 8 ~ 10 times in the coarse powder that obtains of step process, water temperature is 35 ~ 40 DEG C, soaks 60 ~ 90min, then ultrasonic Treatment, filters, and obtain just extract and filtering residue I, by first extract cold preservation, filtering residue proceeds to step 4);
4) in filtering residue I, the ethanol that volume ratio is 50 ~ 70% is added, controlling filtering residue I with the weight/volume of ethanol is 1:7 ~ 1:10mg/mL, lixiviate 1.5 ~ 2 hours at 35 ~ 40 DEG C, filter, obtain filtrate and filtering residue II, with step 2 after filtering residue II is dried) gained coarse powder mix, proceeds to step 3), proceed to step 5) after ethanol is use up in filtrate decompression recovery;
5) at room temperature utilize inorganic ceramic film to filter respectively first for step 3) gained extract and step 4) gained filtrate, gained filtrate merged, enter rapidly concentrator and concentrate, dry, micronizing, sterilizing, obtains Fructus Psoraleae and exempts from fried dose.
Preferably, soaking described in step 3) is carry out dynamic soaking in steeping tank; Described dynamic soaking preferably arranges eccentric rabbling mechanism respectively in the upper and lower of described steeping tank.
Preferably, the process conditions of ultrasonic Treatment described in step 3) are: supersonic frequency 20 ~ 30kHz, power 300 ~ 500W, time 10 ~ 20min, and number of repetition is 2 ~ 3 times.
Preferably, inorganic ceramic film described in step 5) is Al
2o
3ceramic membrane, aperture is 0.1 ~ 0.2 μm, and operation pressure reduction is 0.05 ~ 0.08MPa.
Preferably, concentrated described in step 5) is that filtrate is carried out vacuum-concentrcted, and when being concentrated into 60 ~ 80 DEG C, relative density is the mastic of 1.05 ~ 1.13.
Preferably, micronizing described in step 5) is, through super micron mill, dried for step 5) concentrate is ground into the fine powder that particle diameter is 10 ~ 75 μm.
Present invention also offers the application that above-mentioned preparation method gained Fructus Psoraleae exempts from fried dose, Fructus Psoraleae is exempted from fried dose directly to add in compound preparation, make pharmaceutically acceptable dosage form, comprise decoction, granule, tablet, capsule, injection, powder, unguentum, pill.
Preferably, present invention also offers the application that above-mentioned preparation method gained Fructus Psoraleae exempts from fried dose, Fructus Psoraleae is exempted from fried dose and directly add in compound preparation, make Chinese medicine compound decoction or granule.
Corylifolia L before the extraction step, need be carried out coarse powder by the present invention, and after coarse powder, medical material homogeneous grain diameter, can improve stripping and the extraction efficiency of effective ingredient in subsequent step.
The mode that the present invention adopts water logging and ultrasonic extraction to combine carries out the first extraction of effective ingredient, and then alcohol steep carries out second extraction.Chinese crude drug after coarse powder can fully infiltrate by water logging, and one by water-soluble composition stripping, and two can improve extraction efficiency; The ultrasonic frequency that ultrasonic extraction need control to be suitable for, power and processing time, according to Chinese medicine Characteristics Control extraction time, so both can reduce energy consumption, the stripping of effective ingredient can also be improved, reduce the destruction of effective ingredient.By water logging and ultrasonic Treatment, for follow-up alcohol steep provides extraction conditions preferably, can also reduce the condition such as time, intensity of alcohol steep, protection effective ingredient is not damaged simultaneously; By the organic assembling of above-mentioned technique, the utilization rate of medical material is improved, the stripping of effective ingredient increases.
Compared with prior art, its beneficial effect is in the present invention:
The present invention is by water logging, the ultrasonic combination involving alcohol steep, solve the problem that raw material availability when utilizing conventional single method to extract is low, effective ingredient destroys seriously and extraction efficiency is not high, being prepared into preparation uses more convenient, more adapts to the allegro life of modern people.Whole process control of the present invention is simple, environmental protection, each processing step Synergistic, and make preparation method universality of the present invention stronger, extraction efficiency is higher, and active component obtains available protecting, improves the availability of finished product preferably.
Detailed description of the invention
In order to understand the present invention better, set forth content of the present invention below in conjunction with embodiment is clear further, but protection content of the present invention is not only confined to the following examples.
In the following description, a large amount of concrete details is given to provide more thorough understanding of the invention.But, it will be apparent to one skilled in the art that the present invention can be implemented without the need to these details one or more.In other example, in order to avoid obscuring with the present invention, technical characteristics more well known in the art are not described.
Fructus Psoraleae is fabaceous dry mature fruit, because containing a large amount of oils and fats and carbohydrate content, in leaching process, easily there is saponification and form jelly, be difficult to filter, extraction efficiency is low, so in the preparation process of Fructus Psoraleae extract, how to reduce the generation of this type of reaction, it is the major issue faced in Fructus Psoraleae extract preparation process, the present invention is in years of researches are explored, by updating, the preparation method that test and summary draw, this preparation method extraction efficiency is high, fried dose of efficacy stability exempted from by gained Fructus Psoraleae, use flexibly.
The invention provides the preparation method that a kind of Fructus Psoraleae exempts from fried dose, carry out according to the following steps:
1) corylifolia L is cleaned roguing, less than 60 DEG C dry;
2) get dried corylifolia L and carry out coarse pulverization, cross 20 ~ 40 mesh sieves, obtain coarse powder;
3) through the 2nd) add the water of coarse powder weight 8 ~ 10 times in the coarse powder that obtains of step process, water temperature is 35 ~ 40 DEG C, soaks 60 ~ 90min, then ultrasonic Treatment, filters, and obtain just extract and filtering residue I, by first extract cold preservation, filtering residue proceeds to step 4);
4) in filtering residue I, the ethanol that volume ratio is 50 ~ 70% is added, controlling filtering residue I with the weight/volume of ethanol is 1:7 ~ 1:10mg/mL, lixiviate 1.5 ~ 2 hours at 35 ~ 40 DEG C, filter, obtain filtrate and filtering residue II, with step 2 after filtering residue II is dried) gained coarse powder mix, proceeds to step 3), proceed to step 5) after ethanol is use up in filtrate decompression recovery;
5) at room temperature utilize inorganic ceramic film to filter respectively first for step 3) gained extract and step 4) gained filtrate, gained filtrate merged, enter rapidly concentrator and concentrate, dry, micronizing, sterilizing, obtains Fructus Psoraleae and exempts from fried dose.
In step 1), described drying can adopt arbitrary conventional drying methods; In order to protect effective ingredient better, preferably adopt vacuum drying, by the moisture control of corylifolia L at below 3.0wt%.
In step 2) in, described coarse pulverization can adopt Chinese medicine grinder to carry out, and gained coarse powder carries out cold preservation in dry environment, prevents being exposed to outer Chinese crude drug active substance by pulverizing and is destroyed.
In step 3), described immersion carries out dynamic soaking in steeping tank, and described dynamic soaking arranges eccentric rabbling mechanism respectively in the upper and lower of described steeping tank, fully mixes with water to make coarse powder, be in suspended dispersion, be beneficial to infiltration and subsequent treatment.The process conditions of described ultrasonic Treatment are preferably: supersonic frequency 20 ~ 30kHz, power 300 ~ 500W, time 10 ~ 20min, and number of repetition is 2 ~ 3 times, can accelerate breaking of cell wall like this, strengthen the release of intracellular organic matter, diffusion and dissolving.
In step 4), be preferably, the ethanol that volume ratio is 55% is added in filtering residue I, controlling filtering residue I with the weight/volume of ethanol is 1:8mg/mL, and lixiviate 1.8 hours at 37 DEG C is filtered, obtain filtrate and filtering residue II, filtering residue II is dried (80 ~ 85 DEG C) afterwards with step 2) gained coarse powder mixes, and proceeds to step 3), filtrate decompression reclaims and proceeds to step 5) after ethanol to the greatest extent.
In step 5), described inorganic ceramic film is preferably Al
2o
3ceramic membrane, aperture is 0.1 ~ 0.2 μm, and operation pressure reduction is 0.05 ~ 0.08MPa, through the process of inorganic ceramic membrane microfiltration, can remove the impurity such as tiny float, is further purified by extracting solution.Described concentrating is that filtrate is carried out vacuum-concentrcted, when being concentrated into 60 ~ 80 DEG C, relative density is the mastic of 1.05 ~ 1.13, can carry out in vacuum-concentrcted equipment, vacuum-concentrcted can reduce the boiling point of material, increase the temperature difference of steam and storeroom, improve concentrated speed, thermal loss is few, concentrate at low ambient temperatures, the loss of heat-sensitive materials can be prevented.Described micronizing is, through super micron mill, dried for step 5) concentrate is ground into the fine powder that particle diameter is 10 ~ 75 μm, preferred pulverization conditions is: temperature-30 ~-10 DEG C, time 5 ~ 30min, through micronizing, material is under the effects such as impact, collision, shearing, grinding, dispersion, and overcome solid interior cohesiveness and make material broken, the dissolution of effective ingredient increases further, take assimilation effect better, availability is higher.The present invention there is no particular determination to sterilizing, and can be sterilizing methods well known to those skilled in the art, the present invention preferably adopts low temperature sterilization.
Present invention also offers above-mentioned preparation method gained Fructus Psoraleae and exempt from the fried dose of application in compound preparation, Fructus Psoraleae being exempted from fried dose directly adds in described compound preparation, make pharmaceutically acceptable dosage form, as decoction, granule, tablet, capsule, injection, powder, unguentum or pill.
Below by specific embodiment, those skilled in the art can be implemented.
Embodiment 1
The invention provides the preparation method that a kind of Fructus Psoraleae exempts from fried dose, carry out according to the following steps:
1) corylifolia L is cleaned roguing, 60 DEG C of dryings, control water content≤3.0wt%;
2) get dried corylifolia L and carry out coarse pulverization, cross 30 mesh sieves, obtain coarse powder;
3) through the 2nd) add the water of coarse powder weight 9 times in the coarse powder that obtains of step process, water temperature is 37 DEG C, soaks 75min, then ultrasonic Treatment, process frequency 25kHz, power 400W, time 12min, number of repetition is 3 times, filter, obtain just extract and filtering residue I, by first extract cold preservation, filtering residue proceeds to step 4);
4) in filtering residue I, the ethanol that volume ratio is 55% is added, controlling filtering residue I with the weight/volume of ethanol is 1:8mg/mL, lixiviate 1.8 hours at 37 DEG C, filter, obtain filtrate and filtering residue II, with step 2 after filtering residue II is dried) gained coarse powder mix, proceeds to step 3), proceed to step 5) after ethanol is use up in filtrate decompression recovery;
5) by step 3) gained, just extract and step 4) gained filtrate at room temperature utilize aperture to be the Al of 0.2 μm respectively
2o
3ceramic membrane filters, gained filtrate is merged, enter rapidly vacuum-concentrcted equipment to concentrate, when being concentrated into 80 DEG C, relative density is the mastic of 1.13, dry, and dried mastic is ground into through super micron mill the fine powder that particle diameter is 10 ~ 75 μm, pulverization conditions is temperature-15 DEG C, time 20min, sterilizing, obtains Fructus Psoraleae and exempts from fried dose.
Embodiment 2
The invention provides the preparation method that a kind of Fructus Psoraleae exempts from fried dose, carry out according to the following steps:
1) corylifolia L is cleaned roguing, 50 DEG C of vacuum dryings, control water content≤3.0wt%;
2) get dried corylifolia L and carry out coarse pulverization, cross 20 mesh sieves, obtain coarse powder;
3) through the 2nd) add the water of coarse powder weight 8 times in the coarse powder that obtains of step process, water temperature is 40 DEG C, soaks 90min, then ultrasonic Treatment, process frequency 30kHz, power 300W, time 20min, number of repetition is 3 times, filter, obtain just extract and filtering residue I, by first extract cold preservation, filtering residue proceeds to step 4);
4) in filtering residue I, the ethanol that volume ratio is 70% is added, controlling filtering residue I with the weight/volume of ethanol is 1:9mg/mL, lixiviate 1.5 hours at 40 DEG C, filter, obtain filtrate and filtering residue II, with step 2 after filtering residue II is dried) gained coarse powder mix, proceeds to step 3), proceed to step 5) after ethanol is use up in filtrate decompression recovery;
5) by step 3) gained, just extract and step 4) gained filtrate at room temperature utilize aperture to be the Al of 0.15 μm respectively
2o
3ceramic membrane filters, gained filtrate is merged, enter rapidly vacuum-concentrcted equipment to concentrate, when being concentrated into 60 DEG C, relative density is the mastic of 1.05, dry, and dried mastic is ground into through super micron mill the fine powder that particle diameter is 10 ~ 75 μm, pulverization conditions is temperature-10 DEG C, time 30min, sterilizing, obtains Fructus Psoraleae and exempts from fried dose.
Embodiment 3
The invention provides the preparation method that a kind of Fructus Psoraleae exempts from fried dose, carry out according to the following steps:
1) corylifolia L is cleaned roguing, 60 DEG C of conventional dryings, control water content≤3.0wt%;
2) get dried corylifolia L and carry out coarse pulverization, cross 40 mesh sieves, obtain coarse powder;
3) through the 2nd) add the water of coarse powder weight 10 times in the coarse powder that obtains of step process, water temperature is 35 DEG C, soaks 80min, then ultrasonic Treatment, process frequency 20kHz, power 500W, time 10min, number of repetition is 2 times, filter, obtain just extract and filtering residue I, by first extract cold preservation, filtering residue proceeds to step 4);
4) in filtering residue I, the ethanol that volume ratio is 50% is added, controlling filtering residue I with the weight/volume of ethanol is 1:10mg/mL, lixiviate 2 hours at 35 DEG C, filter, obtain filtrate and filtering residue II, with step 2 after filtering residue II is dried) gained coarse powder mix, proceeds to step 3), proceed to step 5) after ethanol is use up in filtrate decompression recovery;
5) by step 3) gained, just extract and step 4) gained filtrate at room temperature utilize aperture to be the Al of 0.2 μm respectively
2o
3ceramic membrane filters, gained filtrate is merged, enter rapidly vacuum-concentrcted equipment to concentrate, when being concentrated into 75 DEG C, relative density is the mastic of 1.10, dry, and dried mastic is ground into through super micron mill the fine powder that particle diameter is 10 ~ 75 μm, pulverization conditions is temperature-30 DEG C, time 5min, sterilizing, obtains Fructus Psoraleae and exempts from fried dose.
Embodiment 4
The invention provides the preparation method that a kind of Fructus Psoraleae exempts from fried dose, carry out according to the following steps:
1) corylifolia L is cleaned roguing, 60 DEG C of conventional dryings, control water content≤3.0wt%;
2) get dried corylifolia L and carry out coarse pulverization, cross 30 mesh sieves, obtain coarse powder;
3) through the 2nd) add the water of coarse powder weight 8 times in the coarse powder that obtains of step process, water temperature is 40 DEG C, soaks 60min, then ultrasonic Treatment, process frequency 28kHz, power 400W, time 10min, number of repetition is 3 times, filter, obtain just extract and filtering residue I, by first extract cold preservation, filtering residue proceeds to step 4);
4) in filtering residue I, the ethanol that volume ratio is 65% is added, controlling filtering residue I with the weight/volume of ethanol is 1:7mg/mL, lixiviate 1.5 hours at 40 DEG C, filter, obtain filtrate and filtering residue II, with step 2 after filtering residue II is dried) gained coarse powder mix, proceeds to step 3), proceed to step 5) after ethanol is use up in filtrate decompression recovery;
5) by step 3) gained, just extract and step 4) gained filtrate at room temperature utilize aperture to be the Al of 0.1 μm respectively
2o
3ceramic membrane filters, gained filtrate is merged, enter rapidly vacuum-concentrcted equipment to concentrate, when being concentrated into 75 DEG C, relative density is the mastic of 1.11, dry, and dried mastic is ground into through super micron mill the fine powder that particle diameter is 10 ~ 75 μm, pulverization conditions is temperature-10 DEG C, time 25min, sterilizing, obtains Fructus Psoraleae and exempts from fried dose.
Embodiment 5
The invention provides the preparation method that a kind of Fructus Psoraleae exempts from fried dose, carry out according to the following steps:
1) corylifolia L is cleaned roguing, 60 DEG C of conventional dryings, control water content≤3.0wt%;
2) get dried corylifolia L and carry out coarse pulverization, cross 40 mesh sieves, obtain coarse powder;
3) through the 2nd) add the water of coarse powder weight 9 times in the coarse powder that obtains of step process, water temperature is 35 DEG C, soaks 90min, then ultrasonic Treatment, process frequency 20kHz, power 500W, time 15min, number of repetition is 2 times, filter, obtain just extract and filtering residue I, by first extract cold preservation, filtering residue proceeds to step 4);
4) in filtering residue I, the ethanol that volume ratio is 55% is added, controlling filtering residue I with the weight/volume of ethanol is 1:9mg/mL, lixiviate 1.5 hours at 37 DEG C, filter, obtain filtrate and filtering residue II, with step 2 after filtering residue II is dried) gained coarse powder mix, proceeds to step 3), proceed to step 5) after ethanol is use up in filtrate decompression recovery;
5) by step 3) gained, just extract and step 4) gained filtrate at room temperature utilize aperture to be the Al of 0.15 μm respectively
2o
3ceramic membrane filters, gained filtrate is merged, enter rapidly vacuum-concentrcted equipment to concentrate, when being concentrated into 65 DEG C, relative density is the mastic of 1.08, dry, and dried mastic is ground into through super micron mill the fine powder that particle diameter is 10 ~ 75 μm, pulverization conditions is temperature-20 DEG C, time 15min, sterilizing, obtains Fructus Psoraleae and exempts from fried dose.
Below by experiment embodiment, beneficial effect of the present invention is described.Make up a prescription because traditional Chinese medicine formula is generally decoction, between each medicine, interactively is complicated, and adopts and exempt from fried dose and whether can obtain same or better effect, and applicant has done long-term clinical research to this, what the application enumerated is only certain embodiments.
Drug efficacy study
1) to treat the oral traditional Chinese medicine decoction of pathogensis of vitiligo vulgaris
Chinese medicine formula: Radix Rehmanniae 10g, Fructus Corni 12g, Rhizoma Dioscoreae 16g, Herba Euphorbiae Helioscopiae 10g, Poria 15g, Fructus Psoraleae 10g.
Contrast method: get it filled with above-mentioned formula, adds in Decocting pot to decoct and makes decoction I, take according to quantity.
The inventive method: get Radix Rehmanniae 10g, Fructus Corni 12g, Rhizoma Dioscoreae 16g, Herba Euphorbiae Helioscopiae 10g, Poria 15g, add in Decocting pot to decoct and make decoction; By the inventive method, 10g Fructus Psoraleae is made Fructus Psoraleae and exempt from fried dose; Fructus Psoraleae is exempted from fried dose to add in the decoction that all the other Chinese medicines make, stirring and dissolving, makes decoction II.
Lanqin oral solutions:
Choose pathogensis of vitiligo vulgaris patient 120 example of stable phase between 10 ~ 50 years old; Men and women does not limit, and skin lesion area accounts for 5 ~ 10% of whole body surface area; The regular further consultation of patient, omnidistance medication.
Above-mentioned 120 routine patients are divided into 2 groups at random, often organize 60 examples; Respectively by taking decoction I (matched group) and decoction II (experimental group) in identical dosage at one time section, continuous use 2 months is 1 course for the treatment of, observes 1 course for the treatment of.
Evaluation index: recovery from illness: white macula all disappears, recovers normal skin tone; Effective: white macula disappears with newborn pigment island all more than 50%; Effective: white macula disappears less than 50%, newborn pigment island more than 10%; Invalid: white macula is unchanged or reduce, the area recovering normal skin tone accounts for skin lesion area less than 10%.
Shown in statistical result sees the following form:
Table 1 liang group Clinical efficacy comparison
| Group | Number of cases | Recovery from illness | Effective | Effectively | Invalid |
| Matched group | 60 | 13 | 27 | 15 | 5 |
| Experimental group | 60 | 14 | 31 | 13 | 2 |
Found out by table 1; experimental group is better than matched group; namely the decoction II that the inventive method is made is taken; curative effect is better than and decocts decoction I; method of the present invention is adopted not only not reduce drug effect; and better efficacy, this also illustrates that Fructus Psoraleae prepared according to the methods of the invention is exempted from fried dose of its drug effect and maintained better, and active component obtains protection preferably and extracts.
2) to treat the Chinese medicine for oral administration granule of hyperlipemia
Chinese medicine formula: Folium Nelumbinis extract 60g, Radix Salviae Miltiorrhizae extract 16g, Fructus Crataegi extract 45g, Fructus Psoraleae extract 14g, Folium Sennae extract 2g, pharmaceutically acceptable adjuvant.
Contrast method: the extracting method of each Chinese medicine extract is as follows:
The preparation of Folium Nelumbinis extract: (1) adds the ethanol of 4 times 60% after pulverizing, adjust pH12.0, reflux, extract, 2 times at 75 DEG C, each extraction 2 hours, collects extracting solution for subsequent use, (2) by gained Folium Nelumbinis medical material residue in (1), add the ethanol of 4 times 85%, adjust pH3.0, reflux, extract, 3 times at 75 DEG C, each extraction 2 hours, collects extracting solution for subsequent use, (3) by extracting solution in (1) through centrifugal, filter after supernatant, after ethanol is reclaimed in distilling under reduced pressure, regulate pH7.0, again through centrifugal, filtration, get supernatant and be mixed with 3mg/mL solution, pump in AB-8 macroporous resin column and adsorb, applied sample amount is 4BV, after pure water, each 2BV gradient elution of 20% ethanol, use 70% ethanol elution, collect eluent, decompression recycling ethanol, in vacuum 0.06MPa-0.09MPa, evaporation and concentration at 65 DEG C-85 DEG C, obtain concentrated solution for subsequent use, (4) by extracting solution in (2) through centrifugal, supernatant after filtration, after ethanol is reclaimed in distilling under reduced pressure, add 2% hydrochloric acid solution, after ultrasonic dissolution 1h, divide with normal hexane and extract color element for three times, regulate pH value of solution 7.0, centrifugal, filter, regulate supernatant pH13.0, pump in D101 macroporous resin column and adsorb, applied sample amount is 2BV, with pure water, organic solution each 2BV gradient remove impurity of 30% concentration of alcohol, finally use 4BV volume 50% methanol solution eluting, collect 50% methanol-eluted fractions, at vacuum 0.06Mpa-0.09Mpa, evaporation and concentration at 65 DEG C-85 DEG C, obtain concentrated solution for subsequent use, (5) obtained concentrated solution in (3), (4) is merged, through concentrating the obtained Folium Nelumbinis extract of final vacuum drying further.
The preparation of Radix Salviae Miltiorrhizae extract: get Radix Salviae Miltiorrhizae, pulverizes, adds medical material weight 4 times amount 60% alcohol heating reflux at every turn and extract 3 times, each 1 hour; Merge extractive liquid, is 2.8 to let cool with acid for adjusting pH, centrifugal, gets the macroporous resin column that resin volume on clear liquor is equivalent to crude drug 3 times, and first rinse with water, water lotion discards, then uses 90% ethanol elution of 2.5 times of column volumes, collects eluent; Eluent concentrating under reduced pressure, dry, obtain Radix Salviae Miltiorrhizae extract.
The preparation of Fructus Crataegi extract: the Fructus Crataegi of getting pulverizing, adds medical material weight 4 times amount water temperature leaching 2h, heating and refluxing extraction 3 times, each 2 hours, extracting solution concentrating under reduced pressure, centrifugal, get macroporous resin column on clear liquor, first rinse with water, then use 60% ethanol elution, collect ethanol elution; Eluent concentrating under reduced pressure, dry, obtain Fructus Crataegi extract.
The preparation of Fructus Psoraleae extract: get stir-baked SEMEN PSORALEAE with salt solution and pulverize, add 3 times amount 70% ethanol extraction 2 times, extracting solution concentrates, and coldly puts, and filters, collecting precipitation 85% dissolve with ethanol, filters, and filtrate concentrates, and drying, obtains stir-baked SEMEN PSORALEAE with salt solution extract;
The preparation of Folium Sennae extract: get Folium Sennae and pulverize, add the ethanol extraction (temperature 80 DEG C) 2 times of 2 times amount 50%, extracting solution filters, and reclaims ethanol at 80 DEG C, concentrated after filtering, dry Folium Sennae extract at 50 DEG C.
Get above-mentioned obtained Folium Nelumbinis extract 60g, Radix Salviae Miltiorrhizae extract 16g, Fructus Crataegi extract 45g, Fructus Psoraleae extract 14g, Folium Sennae extract 2g mix, add the right amount of auxiliary materials such as low-substituted hydroxypropyl cellulose, make granule I.
The inventive method: the same matched group of preparation method of Folium Nelumbinis extract, Radix Salviae Miltiorrhizae extract, Fructus Crataegi extract, Folium Sennae extract, the preparation method that the preparation method of Fructus Psoraleae extract exempts from fried dose according to the embodiment of the present invention 1 Fructus Psoraleae is carried out, then take Folium Nelumbinis extract 60g, Radix Salviae Miltiorrhizae extract 16g, Fructus Crataegi extract 45g, Fructus Psoraleae exempt from fried dose of 14g, Folium Sennae extract 2g, mixing, add the right amount of auxiliary materials such as low-substituted hydroxypropyl cellulose, make granule II.
Lanqin oral solutions:
Choose 66 routine hyperlipidemia patients, the age is all between 30 ~ 60 years old.
Above-mentioned 66 routine patients are divided into 2 groups at random, often organize 33 examples; Respectively by taking granule I (matched group) and granule II (experimental group) in identical dosage at one time section, continuous use 2 months is 1 course for the treatment of, observes 1 course for the treatment of.Patient stops other associated treatment at treatments period, requires patient's less salt, low fat diet, the amount of keeping on a diet, and keeps moderate exercise.
Observation index: observe Blood Lipid situation after treatment, treats and forward and backwardly measures on an empty stomach serum TC, TG, LDL-C, HDL-C value under base state after 12h respectively
Criterion of therapeutical effect: with reference to curative effect determinate standard in State Food and Drug Administration chief editor " new Chinese medicine guideline of clinical investigations " (2002 editions).Clinic control: the every inspection of laboratory recovers normal.Effective: lipids detection reaches following any one person, TC decline >=20, TG decline >=40, HDL-C rising >=0.26mmol/L.Effective: lipids detection reaches following any one person.TC declines >=109/5 but <20, TG decline >=20 but <40, HDL-C rising >=0.104mmol/L but <0.26mmol/L.Invalid: lipids detection does not reach above standard person.
Shown in statistical result sees the following form:
Table 2 liang group Clinical efficacy comparison
| Group | Number of cases | Effective | Effectively | Invalid |
| Matched group | 33 | 7 | 19 | 7 |
| Experimental group | 33 | 11 | 20 | 2 |
As can be seen from Table 2; experimental group curative effect is better than matched group; namely the granule II that the inventive method is made is taken; curative effect is better than granule I; adopt method better efficacy of the present invention; this also illustrates that Fructus Psoraleae prepared according to the methods of the invention is exempted from fried dose of its drug effect and maintained better, and active component obtains protection preferably and extracts.
Above-mentioned clinical research can obviously be found out, Fructus Psoraleae prepared by the present invention is exempted from fried dose and can be applied to and prepare compound preparation, namely the Chinese medicine decoction, the granule that comprise Fructus Psoraleae fat/Fructus Psoraleae extract can be prepared into, same, also can be prepared into tablet, capsule, injection, powder, unguentum, pill, not enumerate in this application; And evident in efficacy, be obviously better than conventional directly decoction gained Chinese medicine preparation.
Claims (8)
1. the preparation method of fried dose exempted from by Fructus Psoraleae, it is characterized in that, comprises the following steps:
1) corylifolia L is cleaned roguing, less than 60 DEG C dry;
2) get dried corylifolia L and carry out coarse pulverization, cross 20 ~ 40 mesh sieves, obtain coarse powder;
3) through the 2nd) add the water of coarse powder weight 8 ~ 10 times in the coarse powder that obtains of step process, water temperature is 35 ~ 40 DEG C, soaks 60 ~ 90min, then ultrasonic Treatment, filters, and obtain just extract and filtering residue I, by first extract cold preservation, filtering residue proceeds to step 4);
4) in filtering residue I, the ethanol that volume ratio is 50 ~ 70% is added, controlling filtering residue I with the weight/volume of ethanol is 1:7 ~ 1:10mg/mL, lixiviate 1.5 ~ 2 hours at 35 ~ 40 DEG C, filter, obtain filtrate and filtering residue II, with step 2 after filtering residue II is dried) gained coarse powder mix, proceeds to step 3), proceed to step 5) after ethanol is use up in filtrate decompression recovery;
5) at room temperature utilize inorganic ceramic film to filter respectively first for step 3) gained extract and step 4) gained filtrate, gained filtrate merged, enter rapidly concentrator and concentrate, dry, micronizing, sterilizing, obtains Fructus Psoraleae and exempts from fried dose.
2. the preparation method of fried dose exempted from by Fructus Psoraleae as claimed in claim 1, it is characterized in that: soaking described in step 3) is carry out dynamic soaking in steeping tank.
3. the preparation method of fried dose exempted from by Fructus Psoraleae as claimed in claim 1, and it is characterized in that: the process conditions of ultrasonic Treatment described in step 3) are: supersonic frequency 20 ~ 30kHz, power 300 ~ 500W, time 10 ~ 20min, number of repetition is 2 ~ 3 times.
4. the preparation method of fried dose exempted from by Fructus Psoraleae as claimed in claim 1, it is characterized in that: inorganic ceramic film described in step 5) is Al
2o
3ceramic membrane, aperture is 0.1 ~ 0.2 μm, and operation pressure reduction is 0.05 ~ 0.08MPa.
5. the preparation method of fried dose exempted from by Fructus Psoraleae as claimed in claim 1, it is characterized in that: concentrated described in step 5) is that filtrate is carried out vacuum-concentrcted, and when being concentrated into 60 ~ 80 DEG C, relative density is the mastic of 1.05 ~ 1.13.
6. the preparation method of fried dose exempted from by Fructus Psoraleae as claimed in claim 1, it is characterized in that: micronizing described in step 5) is, through super micron mill, dried for step 5) concentrate is ground into the fine powder that particle diameter is 10 ~ 75 μm.
7. the application of fried dose exempted from by Fructus Psoraleae prepared by the preparation method that Fructus Psoraleae exempts from fried dose as described in any one of claim 1 ~ 6, it is characterized in that: Fructus Psoraleae is exempted from fried dose and directly add in compound preparation, make pharmaceutically acceptable dosage form, comprise decoction, granule, tablet, capsule, injection, powder, unguentum, pill.
8. the application of fried dose exempted from by Fructus Psoraleae prepared by the preparation method that Fructus Psoraleae exempts from fried dose as claimed in claim 7, it is characterized in that: Fructus Psoraleae is exempted from fried dose and directly add in compound preparation, make Chinese medicine compound decoction or granule.
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| CN110317109A (en) * | 2019-07-18 | 2019-10-11 | 含山县青峰含翠果业有限公司 | A kind of preparation method of cherry tree special fertilizer that preventing and treating subterranean pest-insect |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101822697A (en) * | 2009-07-13 | 2010-09-08 | 湖南省中医药研究院 | Preparation method of traditional Chinese medicine ultrafine formula particles |
| CN102698033A (en) * | 2012-04-19 | 2012-10-03 | 田杰 | Traditional Chinese medicine composition for treating vitiligo vulgaris |
| CN103705594A (en) * | 2013-12-12 | 2014-04-09 | 南昌济顺制药有限公司 | Traditional Chinese medicine composition for treating hyperlipidemia and preparation method thereof |
-
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101822697A (en) * | 2009-07-13 | 2010-09-08 | 湖南省中医药研究院 | Preparation method of traditional Chinese medicine ultrafine formula particles |
| CN102698033A (en) * | 2012-04-19 | 2012-10-03 | 田杰 | Traditional Chinese medicine composition for treating vitiligo vulgaris |
| CN103705594A (en) * | 2013-12-12 | 2014-04-09 | 南昌济顺制药有限公司 | Traditional Chinese medicine composition for treating hyperlipidemia and preparation method thereof |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110317109A (en) * | 2019-07-18 | 2019-10-11 | 含山县青峰含翠果业有限公司 | A kind of preparation method of cherry tree special fertilizer that preventing and treating subterranean pest-insect |
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Application publication date: 20151223 |