Background technology
Fluorine thiophene worm sulfone (Fluensulfone, CAS accession number: 318290-98-1, chemical name: the fluoro-1,3-thiazoles of 5--2-base-3, the fluoro-3-butene-1 of 3,3-tri--Ji sulfone) be the fluoroolefins nematocide developed by MakhteshimChemicalWorks company.Fluorine thiophene worm sulfone in polypide, can control polypide activity by multiple physiological action, and then paralysis nematode movement; Hinder and reduce egg counts or egg hatch rate, controlling nematode and occur on a large scale; Directly kill nematode by contacting, larva cannot be survived, irreversible nematocidal effect can make nematode dead.
Amino-oligosaccharide (chitosan oligosaccharide) a kind of is first degraded degraded is obtained again after shitosan by chitin, or extracted the low toxicity bactericide obtained by fermentable.This compound not only has extremely strong control and eradicant action to fungi, bacterium, virus, but also has nutrition, adjustment, removing toxic substances, antibacterial effect.Research finds that amino-oligosaccharide can inducing plant disease resistance, the preferably generation of control cucumber root-knot nematode.
Nimbin is the insecticide of wide spectrum recognized in the world, efficient, low toxicity, easily degraded, noresidue, all has to nearly all plant insect, indoor sanitary insect pest etc. the effect of killing, and to people and animals and surrounding environment without any pollution.
Ivermectin is novel wide spectrum, high-efficiency low-toxicity antibiotics antiparasitic agent, the mechanism of action is the release of the inhibitory transmitter γ-aminobutyric acid (GABA) increasing polypide, and open the Cl ion channel of glutamic acid control, strengthen neu to the permeability of Cl, thus the transmission of block nerves signal, play neural paralysis effect, make muscle cell lose contractility, thus cause polypide dead.Good preventive effect is all had to animal parasitic nematodes and agriculture nematode.
The agriculture chemical compounding of reasonable science or mixture can expand insecticidal spectrum, improve control efficiency, reduce dosage, less residue of pesticide, extend the Pesticide use life-span, delay the generation of pest resistance, be conducive to sustainable development.There is no the report about fluorine thiophene worm sulfone nematicidal composition composite with amino-oligosaccharide, nimbin, ivermectin respectively and application thereof at present.
Summary of the invention
In view of this, in order to solve the problem, the invention provides a kind of nematicidal composition of fluorine-containing thiophene worm sulfone.The present invention, through lot of experiments, filters out fluorine thiophene worm sulfone and carries out composite with amino-oligosaccharide, nimbin, ivermectin respectively, and obtained nematicidal composition has obvious synergistic effect, also shows good control efficiency for field trial.
For achieving the above object, the present invention adopts following technical scheme:
The nematicidal composition of fluorine-containing thiophene worm sulfone of the present invention, comprises effective constituents A and active ingredient B; Described effective constituents A is fluorine thiophene worm sulfone, and described active ingredient B is the one in amino-oligosaccharide, nimbin or ivermectin.
Further, the weight ratio of described effective constituents A and active ingredient B is 1 ~ 25:25 ~ 1.
Further, described effective constituents A and active ingredient B account for the percentage by weight of nematicidal composition is 0.1% ~ 80%.
Further, described nematicidal composition also comprises pesticidal preparations auxiliary element, and described pesticidal preparations auxiliary element comprises stabilizing agent, solvent, auxiliary agent and carrier.
Further, described nematicidal composition can make the various formulations being suitable for agricultural production, comprises suspending agent, aqueous emulsion, microemulsion, granule, water dispersible granules, wetting powder.
The application of nematicidal composition in control vegetable crop, cereal crops, lawn, Pomology disease of fluorine-containing thiophene worm sulfone.
Beneficial effect of the present invention is:
The active component of nematicidal composition of the present invention adopts fluorine thiophene worm sulfone to carry out scientific and reasonable composite with the one be selected from amino-oligosaccharide, nimbin and ivermectin, between built reactive composition, there is synergistic function, this nematicidal composition can make the various formulations be agriculturally suitable for, effectively can prevent and treat nematode, solution dosage is large, medication problem frequently, slow down environmental pressure, extend the Pesticide use life-span.
Embodiment
For a better understanding of the present invention, below in conjunction with specific embodiment, the present invention is further illustrated.
The nematicidal composition of fluorine-containing thiophene worm sulfone of the present invention, comprises effective constituents A and active ingredient B; Described effect composition A is fluorine thiophene worm sulfone, and described active ingredient B is the one in amino-oligosaccharide, nimbin or ivermectin; The weight ratio of described effective constituents A and active ingredient B is 1 ~ 25:25 ~ 1; The percentage by weight of described effective constituents A and active ingredient B accounts for 0.1% ~ 80% of nematicidal composition.Indoor virulence test is below adopted to be described:
Toxicity Determination
Dip method: the Cucumber root of picking artificial infection cucumber root-knot nematode, smash with tissue mashing machine, cross 200 orders, 325 orders, 500 order nematode standard screens successively, with the worm's ovum on aseptic water washing 500 mesh sieve, hatch in sterile water, get the larva (2 instar larvae) hatched for the 2nd day and supply test.With liquid-transfering gun from low concentration to high concentration, once draw liquid 3mL and add respectively in test tube, then draw the equivalent nematode suspension 3mL prepared and add in vitro, make liquid and nematode suspension mixed in equal amounts even.Pipette the above-mentioned mixed liquor of certain volume in the aperture of porous biochemical test plate with liquid-transfering gun, add a cover, in 25 DEG C of constant temperature culture.Each process repeats, if sterile water is control treatment for 4 times.Nematode death condition is observed under anatomical lens, the bus borer population of record investigation and verge of death borer population after 48h.Dead nematode is stiff, still can not bending motion with the touching of hair pin.Calculate lethality and corrected mortality.
Probability value according to drug concentration logarithm value and corresponding plant pathogeny line insect corrected mortality does regression analysis, calculates the LC of each medicament
50, and the co-toxicity coefficient (CTC value) of mixture is calculated according to the abundant method of Sun Yun.
Actual measurement toxicity index (ATI)=(standard agent LC
50/ reagent agent LC
50) × 100
In theoretical toxicity index (TTI)=A medicament toxicity index × mixture A percentage composition+B medicament toxicity index × mixture in the percentage composition of B
Co-toxicity coefficient (CTC)=[the theoretical toxicity index (TTI) of mixture actual measurement toxicity index (ATI)/mixture] × 100
The co-toxicity coefficient of built agent: CTC >=120 are judged as synergistic effect; CTC≤80 are judged as antagonism; 80<CTC<120 is judged as summation action.
Table 1 fluorine thiophene worm sulfone and the composite Toxicity Determination result to cucumber root-knot nematode of amino-oligosaccharide
Reagent agent |
Proportioning |
LC50(mg/ml) |
ATI |
TTI |
CTC |
Fluorine thiophene worm sulfone |
— |
4.47 |
100 |
— |
— |
Amino-oligosaccharide |
— |
8.13 |
54.98 |
— |
— |
Fluorine thiophene worm sulfone: amino-oligosaccharide |
1:25 |
6.32 |
70.69 |
56.71 |
124.64 |
Fluorine thiophene worm sulfone: amino-oligosaccharide |
1:10 |
5.26 |
85.01 |
59.07 |
143.91 |
Fluorine thiophene worm sulfone: amino-oligosaccharide |
1:5 |
4.74 |
94.21 |
62.48 |
150.78 |
Fluorine thiophene worm sulfone: amino-oligosaccharide |
1:3 |
4.08 |
109.44 |
66.23 |
165.23 |
Fluorine thiophene worm sulfone: amino-oligosaccharide |
1:2 |
3.60 |
124.15 |
69.99 |
177.39 |
Fluorine thiophene worm sulfone: amino-oligosaccharide |
1:1 |
2.98 |
150.04 |
77.49 |
193.62 |
Fluorine thiophene worm sulfone: amino-oligosaccharide |
2:1 |
2.83 |
157.88 |
84.99 |
185.76 |
Fluorine thiophene worm sulfone: amino-oligosaccharide |
3:1 |
3.10 |
144.02 |
88.74 |
162.28 |
Fluorine thiophene worm sulfone: amino-oligosaccharide |
5:1 |
3.28 |
136.44 |
92.50 |
147.51 |
Fluorine thiophene worm sulfone: amino-oligosaccharide |
10:1 |
3.48 |
128.28 |
95.91 |
133.76 |
Fluorine thiophene worm sulfone: amino-oligosaccharide |
25:1 |
3.55 |
125.92 |
98.27 |
128.14 |
Table 2 fluorine thiophene worm sulfone and azadirachtin complex are to the Toxicity Determination result of cucumber root-knot nematode
Reagent agent |
Proportioning |
LC50(mg/ml) |
ATI |
TTI |
CTC |
Fluorine thiophene worm sulfone |
— |
4.16 |
100 |
— |
— |
Nimbin |
— |
15.74 |
26.43 |
— |
— |
Fluorine thiophene worm sulfone: nimbin |
1:25 |
11.75 |
35.42 |
29.26 |
121.05 |
Fluorine thiophene worm sulfone: nimbin |
1:10 |
9.27 |
44.87 |
33.12 |
135.49 |
Fluorine thiophene worm sulfone: nimbin |
1:5 |
8.51 |
48.90 |
38.69 |
126.38 |
Fluorine thiophene worm sulfone: nimbin |
1:3 |
6.41 |
64.87 |
44.82 |
144.72 |
Fluorine thiophene worm sulfone: nimbin |
1:2 |
5.07 |
82.11 |
50.95 |
161.15 |
Fluorine thiophene worm sulfone: nimbin |
1:1 |
3.53 |
117.72 |
63.21 |
186.23 |
Fluorine thiophene worm sulfone: nimbin |
2:1 |
2.79 |
148.88 |
75.48 |
197.25 |
Fluorine thiophene worm sulfone: nimbin |
3:1 |
2.92 |
142.66 |
81.61 |
174.81 |
Fluorine thiophene worm sulfone: nimbin |
5:1 |
3.05 |
136.31 |
87.74 |
155.36 |
Fluorine thiophene worm sulfone: nimbin |
10:1 |
2.99 |
139.25 |
93.31 |
149.23 |
Fluorine thiophene worm sulfone: nimbin |
25:1 |
3.22 |
129.28 |
97.17 |
133.04 |
Table 3 fluorine thiophene worm sulfone and the composite Toxicity Determination result to cucumber root-knot nematode of ivermectin
Reagent agent |
Proportioning |
LC50(mg/ml) |
ATI |
TTI |
CTC |
Fluorine thiophene worm sulfone |
— |
4.29 |
100 |
— |
— |
Ivermectin |
— |
7.46 |
57.51 |
— |
— |
Fluorine thiophene worm sulfone: ivermectin |
1:25 |
5.42 |
79.08 |
59.14 |
133.72 |
Fluorine thiophene worm sulfone: ivermectin |
1:10 |
4.93 |
86.95 |
61.37 |
141.68 |
Fluorine thiophene worm sulfone: ivermectin |
1:5 |
4.36 |
98.28 |
64.59 |
152.17 |
Fluorine thiophene worm sulfone: ivermectin |
1:3 |
3.76 |
114.02 |
68.13 |
167.35 |
Fluorine thiophene worm sulfone: ivermectin |
1:2 |
3.44 |
124.64 |
71.67 |
173.90 |
Fluorine thiophene worm sulfone: ivermectin |
1:1 |
2.96 |
145.17 |
78.75 |
184.33 |
Fluorine thiophene worm sulfone: ivermectin |
2:1 |
2.52 |
170.01 |
85.84 |
198.06 |
Fluorine thiophene worm sulfone: ivermectin |
3:1 |
2.56 |
167.65 |
89.38 |
187.58 |
Fluorine thiophene worm sulfone: ivermectin |
5:1 |
2.61 |
164.31 |
92.92 |
176.83 |
Fluorine thiophene worm sulfone: ivermectin |
10:1 |
3.08 |
139.12 |
96.14 |
144.71 |
Fluorine thiophene worm sulfone: ivermectin |
25:1 |
3.45 |
124.19 |
98.36 |
126.25 |
As can be seen from table 1,2,3, fluorine thiophene worm sulfone is composite with amino-oligosaccharide, nimbin, ivermectin respectively, in the ratio range of 1 ~ 25:25 ~ 1, co-toxicity coefficient (CTC) is all greater than 120, shows synergistic function to cucumber root-knot nematode.
Nematicidal composition of the present invention can be prepared into the various formulations being suitable for agricultural production, and be below specific embodiment, in formula, percentage is mass percent.
Embodiment 1
6% fluorine thiophene worm sulfone amino-oligosaccharide granule: fluorine thiophene worm sulfone 3%, amino-oligosaccharide 3%, alkylphenol polyoxyethylene 3%, sodium butylnaphthalenesulfonate 2%, 5% polyvinyl alcohol water solution 10%, kaolin complements to 100%.
Embodiment 2
6% fluorine thiophene worm sulfone nimbin granule: fluorine thiophene worm sulfone 4%, nimbin 2%, alkylphenol polyoxyethylene 5%, sodium butylnaphthalenesulfonate 2%, 5% polyvinyl alcohol water solution 10%, kaolin complements to 100%.
Embodiment 3
6% fluorine thiophene worm sulfone ivermectin granule: fluorine thiophene worm sulfone 4%, ivermectin 2%, alkylphenol polyoxyethylene 5%, sodium butylnaphthalenesulfonate 2%, 5% polyvinyl alcohol water solution 10%, kaolin complements to 100%.
Embodiment 4
30% fluorine thiophene worm sulfone ivermectin aqueous emulsion: fluorine thiophene worm sulfone 20%, ivermectin 10%, dimethylbenzene 5%, cyclohexanone 15%, castor oil polyoxyethylene ether 15%, sodium lignin sulfonate 2%, glycerine 1%, xylitol 1%, deionized water supply 100%.
Embodiment 5
24% fluorine thiophene worm sulfone amino-oligosaccharide suspending agent: fluorine thiophene worm sulfone 16%, amino-oligosaccharide 8%, sodium lignin sulfonate 2%, lauryl sodium sulfate 2%, glycerine 1%, xanthans 1%, C-18 fatty alcohol 0.1%, aluminium-magnesium silicate 1%, deionized water supplies 100%.
Embodiment 6
11% fluorine thiophene worm sulfone nimbin suspending agent: fluorine thiophene worm sulfone 10%, nimbin 1%, sodium lignin sulfonate 2%, pull open powder 2%, glycerine 1%, xanthans 1%, silicone oil 0.1%, deionized water supply 100%.
Embodiment 7
55% fluorine thiophene worm sulfone ivermectin wetting powder: fluorine thiophene worm sulfone 30%, ivermectin 25%, naphthalenesulfonate condensation compound 1.5%, lignosulfonates 3%, kaolin stone supply 100%.
Embodiment 8
10% fluorine thiophene worm sulfone micro nimbin emulsion: fluorine thiophene worm sulfone 7%, nimbin 3%, alkylphenol polyoxyethylene 10%, Tween-20 5%, acetone 10%, dimethyl sulfoxide (DMSO) 5%, propane diols 5%, deionized water supply 100%.
Embodiment 9
50% fluorine thiophene worm sulfone amino-oligosaccharide water dispersible granules: fluorine thiophene worm sulfone 25%, amino-oligosaccharide 25%, sodium alkyl benzene sulfonate 3%, polyoxyethylene carboxylate 5%, sodium carboxymethyl starch 2%, ammonium sulfate 5%, diatomite supply 100%.
Embodiment 10
70% fluorine thiophene worm sulfone ivermectin water dispersible granules: fluorine thiophene worm sulfone 50%, ivermectin 20%, sodium alkyl benzene sulfonate 3%, polyoxyethylene carboxylate 5%, soluble corn starch 2%, sodium sulphate 5%, diatomite supply 100%.
Field control effectiveness test
The field experiment of the nematicidal composition of fluorine-containing thiophene worm sulfone of the present invention, for preventing and treating Cucumber root-knot nematode disease:
The result of the test of table 4 each treatment agent field control Cucumber root-knot nematode disease
Treatment agent |
Dosage g.a.i./ha |
30 days preventive effects (%) after medicine |
6% fluorine thiophene worm sulfone GR |
400 |
68.60 |
1% amino-oligosaccharide GR |
1000 |
82.97 |
10% nimbin GR |
1000 |
70.58 |
10% ivermectin GR |
1000 |
72.53 |
6% fluorine thiophene worm sulfone amino-oligosaccharide GR |
800 |
95.84 |
6% fluorine thiophene worm sulfone nimbin GR |
800 |
92.43 |
6% fluorine thiophene worm sulfone ivermectin GR |
800 |
90.86 |
Blank |
— |
— |
Note: in table 4, GR represents granule.6% fluorine thiophene worm sulfone amino-oligosaccharide granule, 6% fluorine thiophene worm sulfone nimbin granule, 6% fluorine thiophene worm sulfone ivermectin granule corresponding embodiment 1-3 respectively.
As can be seen from Table 4, for preventing and treating cucumber root-knot nematode, its successful is better than single dose, does not produce poisoning phenomenon in process of the test, and this composite nematicidal composition has synergistic function, can agriculturally promote the use of.
The above embodiment only have expressed several embodiment of the present invention, and it describes comparatively concrete and detailed, but therefore can not be interpreted as the restriction to the scope of the claims of the present invention.It should be pointed out that for the person of ordinary skill of the art, without departing from the inventive concept of the premise, can also make some distortion and improvement, these all belong to protection scope of the present invention.Therefore, the protection domain of patent of the present invention should be as the criterion with claims.