CN105165388A - Simple inoculum inoculation method - Google Patents
Simple inoculum inoculation method Download PDFInfo
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- CN105165388A CN105165388A CN201510408921.6A CN201510408921A CN105165388A CN 105165388 A CN105165388 A CN 105165388A CN 201510408921 A CN201510408921 A CN 201510408921A CN 105165388 A CN105165388 A CN 105165388A
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- inoculation
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
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Abstract
The invention relates to a simple inoculum inoculation method and belongs to the technical field of inoculum inoculation. The simple inoculum inoculation method is provided for solving technical problems. The technical scheme adopted is as follows: the simple inoculum inoculation method comprises the following steps: (1) building a wind-tight inoculation shed outdoors with plastic cloth, sterilizing the inside and outside of the inoculation shed, and getting sterilized and disinfected inoculation utensils and an inoculum ready in the inoculation shed; (2) chopping carrots in strips, of which both the width and thickness of each are about 1cm, drilling a hole, of which the diameter is 1-2mm, in the middle of each strip, injecting mixed powder of glucose, saccharose and soybean meal, which are in the mass ratio of 2: 2: 1, into each hole, plugging the holes, and then, carrying out steaming, so as to prepare a culture medium; and (3) completing inoculum inoculation work in the inoculation shed built in the step (1) by using the culture medium prepared in the step (2), and transferring the inoculated culture medium into a culturing room for culturing. The culture medium prepared in the step (2) not only can serve as an inoculum amplification culture medium, but also can serve as a core rod of a cultivated species.
Description
Technical field
A kind of bacterial classification Simple inoculation method of the present invention, belongs to strain inoculation technical field.
Background technology
Usual mushroom inoculation work has stricter environmental requirement, as requirements such as humidity, temperature, sterile air, is all generally carry out at special transfer room.Need to carry out strict sterilizing operation to transfer room, ensure that inoculation environment does not have miscellaneous bacteria, prevent bacterial classification from getting dirty.Or always operate at inoculating hood.No matter be the construction of transfer room or the purchase of inoculating hood, all need high expense.This is a no small burden for cultivating on a small scale.
In addition, the expansion of bacterial classification cultivate needed for medium preparation also more loaded down with trivial details, the raw material type of needs is more, and process for preparation lime light is also many, raw materials used some not easily buy at slightly remote sites, also not easily preserve, also bring very large impact to inoculation work.
Summary of the invention
The present invention overcomes the deficiencies in the prior art, and technical problem to be solved is to provide a kind of easy strain inoculation method.
For solving the problems of the technologies described above, the technical solution adopted in the present invention is: a kind of bacterial classification Simple inoculation method, comprises the following steps:
1) inoculation canopy is built
Build airfast inoculation canopy at outdoor use Polypropylence Sheet, carry out sterilizing to inside and outside inoculation canopy, in inoculation canopy, get out inoculating appliance and the bacterial classification of sterilization;
2) medium is prepared
Carrot is cut into and wide and is thickly the rectangular of about 1cm, the hole of middle drill diameter 1-2mm, inject the mixed powder of glucose, sucrose, analysis for soybean powder mass ratio 2:2:1, cook after plug-hole, as medium;
3) inoculate
With step 2) the inoculation canopy built in step 1) of medium in complete strain inoculation work, postvaccinal medium is proceeded to culturing room and cultivates.
Described step 2) obtained medium can not only be used as bacterial classification and expand medium, also can be used as cultivated species plug.
Compared with prior art the present invention has following beneficial effect: the medium that the present invention relates to makes simple, but can provide basic nutriment for the bacterium that sends out of the expansion cultivation of bacterial classification and cultivated species, ensure the growth of bacterial classification health; Although inoculation method of the present invention seems simple and crude, through the inspection of inventor's practice, adopt the method inoculation, when not needing sterilizing chamber, inoculating hood, can ensure bacterial classification survival rate more than 97%, miscellaneous bacteria does not almost have.Therefore, the medium that the present invention relates to and inoculation method, on the basis ensureing bacterial classification survival rate, purity and growing state, greatly simplify the requirement of medium and transfer room, have significant effect.
Embodiment
Below in conjunction with specific embodiment, the invention will be further described.
Embodiment 1
The making of medium
Carrot is cut into and wide and is thickly the rectangular of about 1cm, the hole of middle drill diameter 1-2mm, inject the mixed powder of glucose, sucrose, analysis for soybean powder mass ratio 2:2:1, cook after plug-hole, after cooling, as medium.
Embodiment 2
A kind of bacterial classification Simple inoculation method, comprises the following steps:
1) inoculation canopy is built
Build airfast inoculation canopy at outdoor use Polypropylence Sheet, carry out sterilizing to inside and outside inoculation canopy, in inoculation canopy, get out inoculating appliance and the bacterial classification of sterilization;
2) inoculate
Complete strain inoculation work in the inoculation canopy built in step 1) with the medium of embodiment 1, proceeds to culturing room by postvaccinal medium and cultivates.
Embodiment 3
A kind of cultivated species inoculation method, comprises the following steps:
1) inoculation canopy is built
Build airfast inoculation canopy at outdoor use Polypropylence Sheet, carry out sterilizing to inside and outside inoculation canopy, in inoculation canopy, get out inoculating appliance and the bacterial classification of sterilization;
2) inoculate
Complete strain inoculation work in the inoculation canopy built in step 1) with the medium of embodiment 1, proceeds to culturing room by postvaccinal medium and cultivates.
The present invention can summarize with other the concrete form without prejudice to spirit of the present invention or principal character.Therefore, no matter from which point, above-mentioned embodiment of the present invention all can only be thought explanation of the present invention and can not limit invention, claims indicate scope of the present invention, and scope of the present invention is not pointed out in above-mentioned explanation, therefore, any change in the implication suitable with claims of the present invention and scope, all should think to be included in the scope of claims.
Claims (2)
1. a bacterial classification Simple inoculation method, is characterized in that comprising the following steps:
1) inoculation canopy is built
Build airfast inoculation canopy at outdoor use Polypropylence Sheet, carry out sterilizing to inside and outside inoculation canopy, in inoculation canopy, get out inoculating appliance and the bacterial classification of sterilization;
2) medium is prepared
Carrot is cut into and wide and is thickly the rectangular of about 1cm, the hole of middle drill diameter 1-2mm, inject the mixed powder of glucose, sucrose, analysis for soybean powder mass ratio 2:2:1, cook after plug-hole, as medium;
3) inoculate
With step 2) the inoculation canopy built in step 1) of medium in complete strain inoculation work, postvaccinal medium is proceeded to culturing room and cultivates.
2. a kind of bacterial classification Simple inoculation method according to claim 1, is characterized in that: described step 2) obtained medium is used as bacterial classification and expands medium or cultivated species plug.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510408921.6A CN105165388B (en) | 2015-07-14 | 2015-07-14 | A kind of strain Simple inoculation method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201510408921.6A CN105165388B (en) | 2015-07-14 | 2015-07-14 | A kind of strain Simple inoculation method |
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| Publication Number | Publication Date |
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| CN105165388A true CN105165388A (en) | 2015-12-23 |
| CN105165388B CN105165388B (en) | 2017-09-12 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201510408921.6A Expired - Fee Related CN105165388B (en) | 2015-07-14 | 2015-07-14 | A kind of strain Simple inoculation method |
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2015
- 2015-07-14 CN CN201510408921.6A patent/CN105165388B/en not_active Expired - Fee Related
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| JPH0662661A (en) * | 1992-08-19 | 1994-03-08 | Hokkaido Prefecture | Medium for culturing basidiomycete belonging to genus armillariella |
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| CN101953266A (en) * | 2009-07-14 | 2011-01-26 | 四川省农业科学院 | Plastic bag cultivation method of oyster mushrooms |
| CN101658102A (en) * | 2009-08-20 | 2010-03-03 | 山西山宝食用菌生物有限公司 | Cultivating method of pleurotus ferulae |
| CN101803533A (en) * | 2010-05-06 | 2010-08-18 | 蒋远旺 | Method for cultivating edible fungi |
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| 黄磊等: "食用菌固体培养基的开发与应用综述", 《湖北农业科学》 * |
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| CN105165388B (en) | 2017-09-12 |
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Inventor after: Deng Shuyuan Inventor before: Li Jin |
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Effective date of registration: 20170814 Address after: 041500 Shanxi city of Linfen province Xiangfen County North Gou Xiang iron family member courtyard building 3 unit 101 room 2 Applicant after: Deng Shuyuan Address before: 041500, Shunyi village, West Jia County, Xiangfen County, Shanxi, Linfen Applicant before: XIANGFEN RUIYIPENG EDIBLE FUNGUS PLANTATION SPECIALIZED COOPERATIVES |
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