CN105149332A - Method for remediating heavy metal contaminated soil through combination of microorganism and plant - Google Patents
Method for remediating heavy metal contaminated soil through combination of microorganism and plant Download PDFInfo
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- CN105149332A CN105149332A CN201510638010.2A CN201510638010A CN105149332A CN 105149332 A CN105149332 A CN 105149332A CN 201510638010 A CN201510638010 A CN 201510638010A CN 105149332 A CN105149332 A CN 105149332A
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Abstract
The invention provides a method for remediating heavy metal contaminated soil through combination of a microorganism and a plant. The method comprises the following steps: mixing a magnetotactic bacterium with the heavy metal contaminated soil, irrigating the heavy metal contaminated soil mixed with the magnetotactic bacterium, arranging a magnetic field on the heavy metal contaminated soil, collecting the heavy metal contaminated soil containing the magnetotactic bacterium, washing out the heavy metal contaminated soil, and conducting backfilling on the heavy metal contaminated soil; planting copper weeds in the heavy metal contaminated soil preliminarily remediated by the magnetotactic bacterium, and removing all the copper weeds after 10-12 months; and circularly repeating the steps till the content of heavy metal in soil is up to the safety standard. The method has the advantages that the treatment effect on the heavy metal contaminated soil is good, the operation is simple, the cost is low, heavy metal ions are directly removed, secondary contamination of soil is avoided, and the practicability is strong; moreover, the method is suitable for treating large-area contaminated soil.
Description
Technical field
The present invention relates to contaminated soil and administer field, the particularly a kind of method of microorganism and plant combined restoration of soil polluted by heavy metal.
Background technology
Soil is not only the important component part of earth ecology circle, is also one of the mankind's important natural resources of depending on for existence.Along with the carrying out of the rural activities such as the industrial activity such as exploitation of mineral resources, smelting and sewage irrigation, applying sludge and chemical fertilizer inferior, the harmful heavy metals such as Cd, Pb constantly enter in soil environment, pollute normal productive life.Therefore, administer heavy metal pollution of soil to conservation culture establish and the life of human health significant.
The traditional improvement restorative procedure of heavy metal pollution of soil is generally the method for physics and chemistry, as dilution and covering method, vitrification, heat treatment technics, elution method, electrochemical process etc., although traditional restorative procedure regulation effect is good, last shorter, all there is many defects.The such as cost of traditional administering method is high, not manageability, easily cause secondary pollution etc.
Microorganism remediation utilizes the certain micro-organisms heavy metal in soil to have the effects such as absorption, precipitation, oxidation and reduction, thus reduce the toxicity of heavy metal in soil.This technology can make heavy metal contaminants remove from soil, less to surrounding environment influence, can not produce secondary pollution, the huge soil remediation potentiality had.But the microorganism remediation technology of existing heavy-metal contaminated soil has some limitations, as long in the microorganism remediation duration, take effect slow, the general harm forming complex compound reduction heavy metal, heavy metal can not be removed from soil.
Phytoremediation technology refers to and utilizes plant and root system thereof to the absorption of soil pollutant, volatilization, conversion, degraded, fixation and remove the recovery technique of pollutant in soil.Heavy metal is absorbed by plants, if enter plant edible part, harm humans is healthy.For avoiding the exposure to human body, the research selecting non-consumption commercial crop plants to carry out phytoremediation in recent years more and more causes concern, but the plant restoration method effect reported of great majority not significantly, take effect slow, repairing effect is unstable, add the restriction being subject to weather, soil environment, make the Accumulation of heavy metals amount of these plants limited, thus be restricted in its application.
As mentioned above, in order to administer the soil of heavy metal pollution better, be badly in need of solving following technical problem:
1. cultivate suitable microorganism, its high adsorption capacity for heavy metal, be easy to the feature that is separated, energy for growth is strong, strong to environment-adapting ability.
2. determine that suitable tolerant plant and microbial association use, this plant have plantation difficulty low, be easy to manage, be applicable to pollution in wide area soil, growth rapidly, feature that biomass is large.
3. avoid the situation of change and spoiled soil structure in heavy-metal contaminated soil repairing and treating process.
Summary of the invention
Technical problem to be solved by this invention is, for the deficiencies in the prior art, provide a kind of simple to operate, with low cost, repairing effect is good, not spoiled soil fertility, do not exist soil secondary pollution, be applicable to the microorganism of pollution in wide area soil remediation and the method for plant combined restoration of soil polluted by heavy metal.
For solving the problems of the technologies described above, the technical solution adopted in the present invention is:
A method for microorganism and plant combined restoration of soil polluted by heavy metal, comprises the following steps:
1) soil of magnetotactic bacteria with the thick 15 ~ 18cm in the top layer of heavy-metal contaminated soil is mixed, cultivate 30 ~ 35 days;
2) irrigate the soil of step 1) heavy metal pollution, make heavy-metal contaminated soil keep waterflooding state, and magnetic field is set at the soil top of described heavy metal pollution, keep 36 ~ 43 days;
3) step 2 is collected) soil of the described thick 9 ~ 12cm in heavy-metal contaminated soil top layer;
4) with the heavy-metal contaminated soil collected in water wash step 3), repeat 2 ~ 4 times, then cleaned soil is filled out reposition;
5) plant copper weed above the heavy-metal contaminated soil repaired in step 4), after 10 ~ 12 months, copper weed entirety is removed;
6) execution step 1) ~ step 5 is cycled to repeat) 5 ~ 8 times.
Preferably, in step 1), the inoculative proportion of magnetotactic bacteria is inoculate 9.0 × 10 in the soil of every weight per square meter metallic pollution
9~ 1.0 × 10
10individual magnetotactic bacteria.
Preferably, step 2) described magnetic direction deviates from ground upwards, and magnetic field intensity is 200 ~ 300 Gausses.
Preferably, the method for described plantation copper weed is seedling plantation, and the seedling of the 6 ~ 8cm top layer 3 ~ 5cm degree of depth that tilts 30 degree to place into the soil is planted, and spacing in the rows is 17 ~ 26cm, and line-spacing is 20 ~ 28cm.
Preferably, described copper weed seedling by 15% ~ 20% H
2o
2disinfect, then distilled water washing immersion 12 ~ 15h, full seed and the copper weed seed do not sunk grow up to.
Preferably, the heavy-metal contaminated soil water content of described plantation copper weed remains on 15 ~ 20%.
Preferably, the copper weed removed in step 5) is carried out harmless treatment.
Harmless treatment for from copper weed refine obtain copper, also can through pulverizing after as fertilizer for repairing the soil of scarce copper.
The invention has the beneficial effects as follows:
1. magnetotactic bacteria belongs to Gram-negative bacteria, be micro-aerobic, the facultative or obligate anaerobes of obligate, have and bear oxygen characteristic have along the arrangement of thalline major axis, by the magnetic corpusculum particle chain of film bag quilt in cell, under the influence of a magnetic field can directed movement, and heavy metal has stronger adsorption capacity; Under the effect in earth's magnetic field, magnetotactic bacteria can move to deep under ground, because the active force in earth's magnetic field is very weak, at soil surface, magnetic field is set, and make magnetotactic bacteria concentrate in small size by the direction of controlling magnetic field, step 2) in irrigate the object of heavy-metal contaminated soil be reduce the oxygen content of upper soll layer, create the environment being conducive to magnetotactic bacteria existence.
2. the absorption of magnetotactic bacteria heavy metal ion is adsorbed as master with cell surface, and therefore the speed of magnetotactic bacteria Adsorption of Heavy Metals is very fast, and the heavy metal in the process of its movement effectively in absorption soil, greatly reduces the accumulation of heavy metal in soil; Also can not cause the secondary pollution of soil simultaneously.
3. the leacheate that step 4) of the present invention obtains reclaims heavy metal, and prevent heavy metal from getting back to soil or water body on the one hand, the heavy metal reclaimed on the other hand has very high surcharge, can additional income.The rate of recovery of magnetotactic bacteria reaches more than 90%, and leacheate heavy metal recovery rate reaches more than 99%, is conducive to the reparation of heavy-metal contaminated soil.
4. when existing for detection device for multi metallic elements, there is competitive relation between heavy metal element, magnetotactic bacteria is very low to the adsorption rate of Cu element, and copper weed can adsorb the Cu element in soil simultaneously, just in time can make up the deficiency of magnetotactic bacteria; The plantation difficulty of copper weed is low, is applicable to establishing in large scale, effectively prevent pollutant and again enters food chain, decrease the secondary pollution that can cause in rehabilitating soil to greatest extent as non-consumption plant for rehabilitating soil; The copper weed being adsorbed with a large amount of Cu element can obtain copper by refinement, also can through pulverizing after as fertilizer for repairing the soil of scarce copper.
In a word, the method of microorganism of the present invention and plant combined restoration of soil polluted by heavy metal compared with traditional Remediation of Contaminated Soil, have small investment, technical requirement low, be easy to management, directly by effective to removal of heavy metal ions, the secondary pollution that there is not soil, soil remediation, do not enter food chain, not spoiled soil structure, practical, be applicable to pollution in wide area soil.
Detailed description of the invention
By the following examples feature of the present invention and other correlated characteristic are described in further detail, so that the understanding of technical staff of the same trade:
The extracting method of magnetotactic bacteria: the activated sludge of sewage treatment plant of Suo Jin village, Nanjing is mixed by the volume ratio of l:2 with the enriched medium of being down to room temperature through sterilizing, put into wide-mouth bottle, bottleneck is sealed with sterilized gauze, 28 DEG C of standing lucifuges cultivate 30d, magnetotactic bacteria (concrete reference: Liu Ju is collected with magnetotactic bacteria collector, Zhou Peiguo, Zhang Qisheng. magnetotactic bacteria process is containing the application study [J] of Cu, Zn waste water. environmental science and technology, 2013 (26) 4:20-24).By the magnetotactic bacteria collected according to the inoculum concentration of 15%, be inoculated into enriched medium, static lucifuge, at 23 DEG C, when bacterial concentration, reach 9.0 × 10
9~ 1.0 × 10
10individual/mL time, measure the performance of Adsorption of Heavy Metals, specific as follows:
Get bacterium liquid 10ml, be added to the Cr of 100ml
3+ion concentration 40mg/L, As
3+ion concentration 4mg/L, Cd
3+ion concentration 0.4mg/L, Ni
3+the solution of ion concentration 12mg/L and Cu
3+cultivate 2h at 28 DEG C in the solution of ion concentration 50mg/L, measure Cr in water
3+, As
3+, Cd
3+, Ni
3+and Cu
3+the content of ion, Cr
3+, As
3+, Cd
3+, Ni
3+and Cu
3+ionic adsorption rate reaches more than 80% respectively, namely uses as magnetotactic bacteria of the present invention.Magnetotactic bacteria in the present invention can be separated from the soil of other activated sludge or heavy metal pollution, also can make to be existing disclosed magnetotactic bacteria bacterial strain, as long as its adsorption rate obtains requiring.Used magnetotactic bacteria in following examples screens according to the method described above, repeats no more.
Consisting of of enriched medium: 610mg/LNaNO
3, 600mg/LKH
2pO
4, 1100mg/LHOOCCH
2cH
2cOONa, 3980mg/LFe
2(SO
4)
3, 60mg/L cobalamin, 600mg/LK
2hPO
4, 500mg/L sodium thioglycolate.
Adopt contaminated by heavy metals thickness of soil to be 40cm, area is 100m
2soil as Experimental Area, and monoblock is tested field and carry out sample prescription division, length and width are 5m, and stay the wide ridge for 0.3m between each sample prescription, each sample prescription arranges 6 sampled points according to diagonal principle.Collect the soil of top layer, experimental plot 0 ~ 40cm, shady and cool ventilation dries, and removes impurity.Measure soil pH be 7.2, heavy metal Cr, As, Cd, Ni, Cu content in soil respectively: the concentration of Cr element is 569mg/kg
-1, the concentration of As element is 50mg/kg
-1, the concentration of Cd element is 1.8mg/kg
-1, the concentration of Ni element is 189mg/kg
-1, the concentration of Cu element is 692mg/kg
-1.
According to national standard of soil environment quality GB15618-2008, when soil pH=6.5 ~ 7.5, nonirrigated farmland, agricultural land, vegetable plot heavy metal Cr, As, Cd, Ni and Cu secondary standard value are respectively 200,25,0.45,90,100mg/kg, therefore, there is more serious heavy metal pollution in above-mentioned monoblock experiment field.
Embodiment 1
A method for microorganism and plant combined restoration of soil polluted by heavy metal, comprises the following steps:
1) mixed by the soil of the magnetotactic bacteria of above-mentioned cultivation with the thick 15cm in heavy-metal contaminated soil top layer, the adding proportion of magnetotactic bacteria is inoculate 9.0 × 10 in every square metre of heavy-metal contaminated soil
9individual magnetotactic bacteria, cultivates 30 days;
2) heavy-metal contaminated soil mixed with magnetotactic bacteria is irrigated, make heavy-metal contaminated soil keep waterflooding state, and arrange hot-wire coil generation magnetic field at the soil top of this heavy metal pollution, magnetic direction deviates from ground upwards, magnetic field intensity is 200 Gausses, keeps 36 days;
3) collecting heavy-metal contaminated soil skin depth in step 3) is the soil of 9cm;
4) with the heavy-metal contaminated soil that water wash step 4) is collected, repeat 3 times, then cleaned soil is filled out reposition, leacheate reclaims heavy metal;
5) with 15% H
2o
2thimerosal process copper weed seed, 12h is soaked in distilled water washing, selects full seed and the copper weed seed do not sunk as the seed of copper weed seedling culture;
6) by the seedling of copper weed seed at indoor cultivation 6 ~ 8cm, and tilt gained seedling 30 degree to imbed the heavy-metal contaminated soil top layer 3cm depth of step 5) process, spacing in the rows is 17cm, and line-spacing is 20cm;
7) irregularly to water to the copper weed of plantation, make the soil moisture content of heavy metal pollution remain on 15%; After 10 months, copper weed entirety is removed;
8) execution step 1) ~ step 8 is cycled to repeat) 8 times.
Result shows: be compared to native soil, soil pH rises to 7.5, the concentration of heavy metal Cr, As, Cd, Ni and Cu is respectively 198.12,24.69,0.43,89.91 and 99.83mg/kg, the concentration of heavy metal in soil Cr, As, Cd, Ni and Cu have dropped 65.48% respectively, 50.62%, 76.11%, 52.43%, 85.57%.
Embodiment 2
A method for microorganism and plant combined restoration of soil polluted by heavy metal, comprises the following steps:
1) soil being 17cm by the magnetotactic bacteria of above-mentioned cultivation and heavy-metal contaminated soil skin depth mixes, and the adding proportion of magnetotactic bacteria is inoculate 9.5 × 10 in every square metre of heavy-metal contaminated soil
9individual magnetotactic bacteria, cultivates 33 days;
2) heavy-metal contaminated soil mixed with magnetotactic bacteria is irrigated, make heavy-metal contaminated soil keep waterflooding state, and arrange hot-wire coil generation magnetic field at the soil top of this heavy metal pollution, magnetic direction deviates from ground upwards, magnetic field intensity is 250 Gausses, keeps 39 days;
3) collecting heavy-metal contaminated soil skin depth in step 3) is the soil of 10cm;
4) with the heavy-metal contaminated soil that water wash step 4) is collected, repeat 2 times, then cleaned soil is filled out reposition, leacheate reclaims heavy metal;
5) with 18% H
2o
2thimerosal process copper weed seed, then 13h is soaked in distilled water washing, selects full seed and the copper weed seed do not sunk as the seed of copper weed seedling culture;
6) copper weed seed is trained the seedling of 6 ~ 8cm in indoor, and gained is tilted 30 degree to imbed the heavy-metal contaminated soil top layer 4cm depth of step 5) process without insect pest seedling, spacing in the rows is 19cm, and line-spacing is 25cm;
7) irregularly to water to the copper weed of plantation, make the soil moisture content of heavy metal pollution remain on 18%; After 11 months, copper weed entirety is removed;
8) execution step 1) ~ step 8 is cycled to repeat) 6 times.
Result shows: be compared to native soil, soil pH rises to 7.4, the concentration of heavy metal Cr, As, Cd, Ni and Cu is respectively 202.6,48.9,0.20,66.7 and 16.5mg/kg, the concentration of heavy metal in soil Cr, As, Cd, Ni and Cu have dropped 68.61% respectively, 54.74%, 76.67%, 53.65%, 86.14%.
Embodiment 3
A method for microorganism and plant combined restoration of soil polluted by heavy metal, comprises the following steps:
1) soil being 18cm by the magnetotactic bacteria of above-mentioned cultivation and heavy-metal contaminated soil skin depth mixes, and the adding proportion of magnetotactic bacteria is inoculate 1.0 × 10 in every square metre of heavy-metal contaminated soil
10individual magnetotactic bacteria, cultivates 35 days;
2) heavy-metal contaminated soil mixed with magnetotactic bacteria is irrigated, make heavy-metal contaminated soil keep waterflooding state, and arrange hot-wire coil generation magnetic field at the soil top of this heavy metal pollution, magnetic direction deviates from ground upwards, magnetic field intensity is 300 Gausses, keeps 43 days;
3) collecting heavy-metal contaminated soil skin depth in step 3) is the soil of 12cm;
4) with the heavy-metal contaminated soil that water wash step 4) is collected, repeat 4 times, then cleaned soil is filled out reposition, leacheate reclaims heavy metal;
5) with 20% H
2o
2thimerosal process copper weed seed, 15h is soaked in distilled water washing, selects full seed and the copper weed seed do not sunk as the seed of copper weed seedling culture;
6) copper weed seed is trained the seedling of 6 ~ 8cm in indoor, and gained is tilted 30 degree to imbed the heavy-metal contaminated soil top layer 5cm depth of step 5) process without insect pest seedling, spacing in the rows is 26cm, and line-spacing is 28cm;
7) irregularly to water to the copper weed of plantation, make the soil moisture content of heavy metal pollution remain on 20%; After 12 months, copper weed entirety is removed;
8) execution step 1) ~ step 8 is cycled to repeat) 5 times.
Result shows: be compared to native soil, soil pH rises to 7.6, the concentration of heavy metal Cr, As, Cd, Ni and Cu is respectively 158.52,20.67,0.39,82.57 and 92.86mg/kg, the concentration of heavy metal in soil Cr, As, Cd, Ni and Cu have dropped 72.14% respectively, 58.66%, 78.33%, 56.31%, 86.58%.
Embodiment 4:
There is provided a kind of method of microorganism and plant combined restoration of soil polluted by heavy metal in the present embodiment, the difference of its detailed process and embodiment 1 is only that in step 3, magnetic field intensity is 300 Gausses, and other each steps are identical with embodiment 1.
Result shows: be compared to native soil, soil pH rises to 7.5, the concentration of heavy metal Cr, As, Cd, Ni and Cu is respectively 198.25,23.97,0.43,89.06 and 98.89mg/kg, the concentration of heavy metal in soil Cr, As, Cd, Ni and Cu have dropped 65.12% respectively, 52.06%, 76.11%, 52.88%, 85.71%.
From the reparation result of embodiment 1 ~ 4, in heavy-metal contaminated soil, magnetotactic bacteria and copper weed can reduce the content of Cr, As, Cd, Ni and Cu heavy metal in soil greatly through the combine d bioremediation test of 5 ~ 8 years, the content of Cr, As, Cd, Ni and Cu heavy metal in soil is made to reach safety standard, solve Metal uptake inefficiency, heavy metal repairs that element is single, repair time is oversize, plant is difficult to a difficult problem of surviving, also avoid changing in heavy-metal contaminated soil repairing and treating process and the situation of spoiled soil structure simultaneously.
In order to outstanding innovative point of the present invention, enable those skilled in the art to fully understand the present invention, now enumerate the present invention experimental stage or the comparative example adopting prior art, and effect explanation is carried out to itself and embodiments of the invention.
Comparative example 1:
In above-mentioned experimental plot, only implement magnetotactic bacteria, the soil being 18cm by magnetotactic bacteria and heavy-metal contaminated soil skin depth mixes, and the adding proportion of magnetotactic bacteria is inoculate 1.0 × 10 in the soil of every weight per square meter metallic pollution
10individual magnetotactic bacteria, cultivates 35 days; Irrigate above-mentioned heavy-metal contaminated soil, make heavy-metal contaminated soil keep waterflooding state, and arrange hot-wire coil generation magnetic field at the soil top of this heavy metal pollution, magnetic direction deviates from ground upwards, and magnetic field intensity is 200 Gausses, keeps 43 days; Collecting skin depth is the heavy-metal contaminated soil of 11cm; With the heavy-metal contaminated soil that clear water drip washing is collected, repeat drip washing 4 times, then cleaned soil is filled out reposition, leacheate reclaims heavy metal; Be cycled to repeat and perform above-mentioned steps 8 times, the content of soil pH value and heavy metal Cr, As, Cd, Ni and Cu is measured.
Comparative example 2
In above-mentioned experimental plot, only plant copper weed, the time of plantation copper weed is 8 cycles, removes copper weed for one-period to plant from copper weed.
Select the H by 20%
2o
2disinfect, 15h is soaked in distilled water washing, selects full seed and the copper weed seed sunk, is trained the seedling of 5 ~ 7cm under indoor; Select 6cm without insect pest seedling inclination 30 degree of top layer 5cm depths of placing into the soil, spacing in the rows is 20cm, and line-spacing is 25cm, and weeding of irregularly watering makes the soil moisture content of heavy metal pollution remain on 20%; After 12 months, copper weed entirety is removed; Be cycled to repeat and perform above-mentioned steps 8 times, the content of soil pH value and heavy metal in soil Cr, As, Cd, Ni and Cu is analyzed.
Comparative example 3
In above-mentioned experimental plot, first plant copper weed, then introduce magnetotactic bacteria;
1) with 19% H
2o
2thimerosal process copper weed seed, 14h is soaked in distilled water washing, selects full seed and the copper weed seed do not sunk as the seed of copper weed seedling culture;
2) copper weed seed is trained the seedling of 6cm in indoor, and is tilted 30 degree to imbed heavy-metal contaminated soil top layer 5cm depth without insect pest seedling by gained, spacing in the rows is 26cm, and line-spacing is 28cm;
3) irregularly to water to the copper weed of plantation, make the soil moisture content of heavy metal pollution remain on 20%; After 12 months, copper weed entirety is removed;
4) soil being 17cm by magnetotactic bacteria and the skin depth of heavy-metal contaminated soil mixes, and the adding proportion of magnetotactic bacteria is inoculate 1.0 × 10 in every square metre of heavy-metal contaminated soil
10individual magnetotactic bacteria, cultivates 34 days;
5) heavy-metal contaminated soil mixed with magnetotactic bacteria is irrigated, make heavy-metal contaminated soil keep waterflooding state, and arrange hot-wire coil generation magnetic field at the soil top of this heavy metal pollution, magnetic direction is parallel to sensing west side, ground, magnetic field intensity is 260 Gausses, keeps 42 days;
6) collecting heavy-metal contaminated soil skin depth is the soil of 12cm;
7) with the heavy-metal contaminated soil that water wash step 7) is collected, repeat 4 times, then cleaned soil is filled out reposition, leacheate reclaims heavy metal;
8) execution step 1) ~ step 8 is cycled to repeat) 7 times.
To soil pH value and and the content of heavy metal in soil Cr, As, Cd, Ni and Cu carry out test analysis.
Soil remediation effect:
According to national standard of soil environment quality GB15618-2008, when soil pH=6.5 ~ 7.5, nonirrigated farmland, agricultural land, vegetable plot heavy metal Cr, As, Cd, Ni and Cu secondary standard value are respectively 200,25,0.45,90,100mg/kg.The effect of the soil remediation method of heavy metal pollution described in the embodiment of the present invention 1 ~ 4 and comparative example 1 ~ 3 is evaluated from P in soil H value and the final content of heavy metal (mg/kg) two aspects and checked, as shown in table 1.
The testing result of table 1 embodiment and comparative example
As can be seen from Table 1,1) comparative example 1 ~ 4, after the reparation of 5 ~ 8 years, the content of heavy metal in soil is all below national standard of soil environment quality secondary standard value, can complete the reparation to heavy metal-polluted soil in a short period of time; 2) embodiment 1 and embodiment 4 show that the impact of the size of magnetic field intensity on magnetotactic bacteria adsorption rate is little; 3) comparative example 1 ~ 2 is compared with embodiment 1 ~ 4, no matter which reduces administer step, all can decline, show that the synergic adjustment effect between various administering method is inseparable to the regulation effect of soil.4) comparative example 3 is compared with embodiment 1 ~ 4, and the adjustment of administering step can cause the repairing effect of heavy-metal contaminated soil to be deteriorated.
Clearly, all any amendments done according to technical support essence of the present invention and change still belong in the scope that the technology of the present invention supports for every claim that the method for a kind of microorganism of the present invention and plant combined restoration of soil polluted by heavy metal is addressed and technical support.
Claims (7)
1. a method for microorganism and plant combined restoration of soil polluted by heavy metal, is characterized in that, comprises the following steps:
1) soil of magnetotactic bacteria with the thick 15 ~ 18cm in the top layer of heavy-metal contaminated soil is mixed, cultivate 30 ~ 35 days;
2) irrigate the soil of step 1) heavy metal pollution, make heavy-metal contaminated soil keep waterflooding state, and magnetic field is set at the soil top of described heavy metal pollution, keep 36 ~ 43 days;
3) step 2 is collected) soil of the described thick 9 ~ 12cm in heavy-metal contaminated soil top layer;
4) with the heavy-metal contaminated soil collected in water wash step 3), repeat 2 ~ 4 times, then cleaned soil is filled out reposition;
5) plant copper weed above the heavy-metal contaminated soil repaired in step 4), after 10 ~ 12 months, copper weed entirety is removed;
6) execution step 1) ~ step 5 is cycled to repeat) 5 ~ 8 times.
2. a kind of method of microorganism and plant combined restoration of soil polluted by heavy metal as claimed in claim 1, is characterized in that: in step 1), the inoculative proportion of magnetotactic bacteria is inoculate 9.0 × 10 in the soil of every weight per square meter metallic pollution
9~ 1.0 × 10
10individual magnetotactic bacteria.
3. a kind of method of microorganism and plant combined restoration of soil polluted by heavy metal as claimed in claim 1, is characterized in that: step 2) described magnetic direction deviates from ground upwards, and magnetic field intensity is 200 ~ 300 Gausses.
4. a kind of method of microorganism and plant combined restoration of soil polluted by heavy metal as claimed in claim 1, it is characterized in that: the method for described plantation copper weed is seedling plantation, the seedling of the 6 ~ 8cm top layer 3 ~ 5cm degree of depth that tilts 30 degree to place into the soil is planted, and spacing in the rows is 17 ~ 26cm, and line-spacing is 20 ~ 28cm.
5. the method for a kind of microorganism as claimed in claim 4 and plant combined restoration of soil polluted by heavy metal, is characterized in that: described copper weed seedling by 15% ~ 20% H
2o
2disinfect, then distilled water washing immersion 12 ~ 15h, full seed and the copper weed seed do not sunk grow up to.
6. the method for a kind of microorganism as claimed in claim 1 and plant combined restoration of soil polluted by heavy metal, is characterized in that: the heavy-metal contaminated soil water content of described plantation copper weed remains on 15 ~ 20%.
7. the method for a kind of microorganism as claimed in claim 1 and plant combined restoration of soil polluted by heavy metal, is characterized in that: the copper weed removed in described step 5) carries out harmless treatment.
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