CN106269825A - A kind of plant bionic and the method for microbial association repairing polluted soil - Google Patents

A kind of plant bionic and the method for microbial association repairing polluted soil Download PDF

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Publication number
CN106269825A
CN106269825A CN201610842846.9A CN201610842846A CN106269825A CN 106269825 A CN106269825 A CN 106269825A CN 201610842846 A CN201610842846 A CN 201610842846A CN 106269825 A CN106269825 A CN 106269825A
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China
Prior art keywords
soil
plant
plant bionic
microorganism
prosthetic device
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CN201610842846.9A
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Chinese (zh)
Inventor
周建强
徐愿坚
韩君
杨立鹏
高涛
陈卫文
周敏
何玉龙
张如意
王蕾
凡祖伟
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Beijing Xinyuan Environment Co Ltd
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Beijing Xinyuan Environment Co Ltd
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Priority to CN201610842846.9A priority Critical patent/CN106269825A/en
Publication of CN106269825A publication Critical patent/CN106269825A/en
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    • BPERFORMING OPERATIONS; TRANSPORTING
    • B09DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
    • B09CRECLAMATION OF CONTAMINATED SOIL
    • B09C1/00Reclamation of contaminated soil
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B09DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
    • B09CRECLAMATION OF CONTAMINATED SOIL
    • B09C1/00Reclamation of contaminated soil
    • B09C1/10Reclamation of contaminated soil microbiologically, biologically or by using enzymes
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B09DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
    • B09CRECLAMATION OF CONTAMINATED SOIL
    • B09C2101/00In situ

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  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Soil Sciences (AREA)
  • Environmental & Geological Engineering (AREA)
  • Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Mycology (AREA)
  • Processing Of Solid Wastes (AREA)

Abstract

A kind of plant bionic and the method for microbial association repairing polluted soil, belong to technical field of soil remediation, mainly comprise the steps that (1) levels land, investigation soil pollution situation and the spatial distribution of pollutant thereof;(2) determine the concentration of target contaminant, and repair target;(3) select the filler for target contaminant with preferable enrichment, make plant bionic prosthetic device, carry out plant and free reparation;(4) screening can convert or degrade target contaminant microorganism or add inoculating microbe to change the existing forms of metal in soil, degradable organic pollutant matter, be used in combination with plant bionic recovery technique, improve soil remediation efficiency;(5) plant bionic prosthetic device and the recycling of filler.The concentration of pollutant in soil matter of quickly degrading.This combined remediation technology have cost relatively low, fast and safely, the feature of non-secondary pollution, be a kind of can the recovery technique of popularization and application on a large scale.

Description

A kind of plant bionic and the method for microbial association repairing polluted soil
Technology neighborhood
The invention belongs to technical field of soil remediation, be specifically related to the combined remediation method of contaminated soil.
Background technology
Soil be the mankind depend on for existence natural environment basis, agricultural production, grain security, resources and environment problems etc. all with Soil is closely bound up.In recent years, China's rapid industrial development, problem of environmental pollution increases the weight of the most therewith, and many places all occur in that Serious soil contamination problem.In recent years, China increases the control to soil pollution, has put into effect a series of policy Encouraging and development soil remediation work, 2016.5.28 State Council has issued " Soil Pollution Control action plan ", for promoting China Soil remediation work carries out having stepped a huge step.But due to contaminated soil itself exist persistency, potentiality, refractory reason The features such as property so that contaminated soil is difficult to quickly repair.
For Mu Qian, the recovery technique of contaminated soil mainly includes three major types: peripheral doses, chemical redemption, biological restoration with And the associating of three kinds of recovery techniques.Peripheral doses can quickly remove pollutant, but spends higher, simultaneously need to expend big The manpower and materials of amount;Chemical redemption can remove the polluter in soil thoroughly, but easily causes during chemical redemption Secondary pollution, the structure of destruction soil;Biological restoration is the focus of current soil remediation research, and it is that green ecological can be by people Generally accepted recovery technique, but biological restoration typical time is longer, simultaneously by the shadow of the condition such as soil relief, weather Ring.
The most self-holding recovery technique of contaminated soil based on plant bionic is a kind of novel soil restoring technology, mainly leads to Cross the transpiration of simulating plant, drive the heavy metal in the soil liquid and organic pollution jointly to migrate, pass through prosthetic device Interior filling adsorption fixed-contamination material, thus reduce the concentration of soil pollutant.The available transpiration of plant bionic recovery technique Operating is held in effect realization, it is not necessary to additionaling power;Easy and simple to handle, save artificial, can be according to pollution condition, the difference of processing area Select the prosthetic device of different size specification;The use of plant bionic recovery technique will not cause the notable change of soil physico-chemical property Change, it is simple to the rear utilization of rehabilitating soil;Filler in prosthetic device can secondary resource or without diffusion focus on, it is not necessary to use A large amount of chemical drugss, safety and environmental friendliness;This technical costs advantage is relatively strong, and price is less than conventional physical chemical remediation technology, Operation easier is low, it is easy to carry out on a large scale, and later maintenance is less, it is simple to use;This technological break-through traditional plant recovery technique Present in plant growing slowly, repairing efficiency length, plant absorption pollutant kind be single, weather relies on strong and needs phase Close the limitation such as system control and protection process;Can be combined with other soil restoring technology simultaneously, increase plant and free reparation Efficiency.The present invention utilizes plant bionic recovery technique to combine with microorganism remediation exactly, utilizes microorganism dirty to change soil The existing forms of dye thing or degraded, absorption, fixing soil polluter, thus improve the efficiency of soil remediation.
Summary of the invention
The invention provides the new technique of a kind of plant bionic and microbial association repairing polluted soil, i.e. plant to free and repair Combine with microorganism remediation again, the concentration of pollutant in soil matter of quickly degrading.This combined remediation technology has cost relatively Low, fast and safely, the feature of non-secondary pollution, be a kind of can the recovery technique of popularization and application on a large scale.
A kind of plant bionic and the method for microbial association repairing polluted soil, comprise the following steps:
(1) level land, investigation soil pollution situation and the spatial distribution of pollutant thereof;
(2) concentration of target contaminant is determined, according to the character (pollutant kind) of pollutant, concentration, Soil Utilization class Types etc. determine recovery scenario, including the microorganism determining the schemes such as the distribution of plant bionic prosthetic device, classification and needs The scheme such as kind and distribution;As included, the target contaminant of different locus is different, concentration is different, the plant bionic of employing Prosthetic device filler is different, plant bionic prosthetic device distribution density is different, the kind of microorganism is different;
(3) select the filler for target contaminant with enrichment, make plant bionic prosthetic device, according to step (2) scheme about plant bionic prosthetic device of repairing in target carries out the plant bionic reparation of contaminated soil;
(4) during step (3) plant bionic prosthetic device is repaired, screening can convert or degrade target The microorganism of pollutant or add inoculating microbe, the technical scheme repaired in target about microorganism according to step (2) exists Arranging in soil again and add microorganism, changing the existing forms of metal in soil, degradable organic pollutant matter, microorganism is with planting Thing bionics prosthesis technical tie-up uses, and improves soil remediation efficiency;
(5) plant bionic prosthetic device and the recycling of filler.
Described step (2) determining, the concentration of polluter and character thereof are to formulate the important step of recovery scenario, pollutes The concentration of thing and distribution characteristics thereof determine the distribution density of plant bionic prosthetic device, insert the degree of depth etc. of soil, imitate plant Raw reparation is most important.
After the concentration of target contaminant, reparation, the use pattern of soil decides the reparation of contaminated soil in actual applications Target and scheme.
In described step (3), plant bionic repairs the selection requirement of filler can be enriched with this target stains material, will pollute Material is fixed among filler, requires that polluter can be desorbed by acid, alkali or heat etc. so that filler can reclaim simultaneously Utilize.
In described step (4), the selection of soil microorganism has important effect to the combine d bioremediation of contaminated soil.Microorganism Be chosen to make polluted heavy metals in target contaminant be changed into the dissolved by combined state form or/and increase target stains The dissolubility of soil organic pollutant matter in thing.Microorganism for combine d bioremediation is preferably from local soil microorganism, warp It is used for soil microorganism reparation after crossing screening, so will not be affected by soil types and character.Can also add simultaneously The degrading microorganism of external source characteristic contamination, promotes the fast degradation of soil contaminant.
In described step (4), the addition opportunity of step (4) microorganism and addition are typically based on reparation final goal can be with Time add.
The effect of microorganism mainly includes two aspects, and one is the existing forms for changing soil contaminant, increases The efficiency that plant bionic is repaired;Soil microorganism can make polluted heavy metals be changed into the dissolved by combined state form, it is also possible to Increase the dissolubility of soil organic pollutant matter, combine with plant bionic recovery technique, reduce the concentration of soil contaminant.Two It is to utilize the organic pollution materials in the absorption of microorganism, metabolic process degraded soil or absorption, fixing, a complexation soil huge sum of money Belong to, reduce the toxicity of heavy metal-polluted soil.
In described step (5), in plant bionic prosthetic device, the analytic method of filler depends primarily on characteristic contamination Character, desorbs polluter according to different choice acid, alkali, heat or the alternate manner of its character, it is achieved filler recyclable Utilize.
The present invention is compared with other recovery techniques, and major advantage is:
(1) recovery technique of two kinds of green ecologicals is combined, repair process will not be produced secondary pollution problem, The basic structure of soil will not be destroyed simultaneously, cause secondary pollution.
(2) present invention mainly utilizes plant bionic recovery technique to reduce soil pollution substrate concentration, plant bionic recovery technique Without additionaling power, can realize oneself and continue, repair time is short simultaneously, efficiency is high, environmentally safe, is a kind of environmental friendliness Recovery technique.
(3) plant frees to repair mainly to have the soluble heavy metal in soil and organic pollution and well removes effect Really, poor to the Organic substance effect of the heavy metal of combined state, insoluble, microorganism remediation can be good at making up plant and frees The weakness repaired, utilizes microorganism to change the form of heavy metal-polluted soil, increase the dissolubility of slightly solubility organic pollution materials, thus Improve the efficiency that plant bionic is repaired.
(4) present invention is rapidly and efficiently, to solve traditional microbiological longer for repair time, the deficiency that remediation efficiency is low.
Detailed description of the invention
Below in conjunction with embodiment, the invention will be further described, but the present invention is not limited to following example.
Example 1
Take heavy metal Cd, Pb, Zn contaminated soil uses plant bionic reparation respectively and plant bionic reparation and microorganism Combined remediation technology, repairing efficiency is 1 month, in triplicate, the monthly situation of change of sample analysis heavy metal content in soil.Knot Fruit is as shown in table 1.
Plant frees reparation filler used and 3 heavy metal species is all had preferable concentration effect, it is possible to Sync enrichment Cd, Pb, Zn tri-heavy metal species.Microorganism remediation uses the method adding external source strain, adds fixed nitrogen clostridium in contaminated soil (Clostridium SP.) is with plant bionic recovery technique renovation of heavy metal polluted soil with combined.
Table 1 heavy-metal contaminated soil repairing effect contrasts
In above-mentioned plant bionic reparation and plant bionic microbial association repairing test, background is the same, the plant of employing Bionics prosthesis scheme is the same, and i.e. as material, density, distribution etc., but plant bionic microbial association is repaired at plant bionic Repair process adds fixed nitrogen clostridium (Clostridium SP.), thus adds the efficiency of soil remediation.
As seen from the experiment, plant bionic recovery technique and plant bionic microbial association recovery technique can reduce Cd, Pb, Zn tri-concentration of heavy metal species in soil, but the efficiency that two kinds of recovery techniques reduce is different.The identical time is inline Close and repair can make that heavy metal-polluted soil reduces more, reach the requirement of standard of soil environment quality, it was demonstrated that this invention excellent More property.
After off-test recycling plant bionic prosthetic device, the filler in plant bionic prosthetic device uses The hydrochloric acid solution of 0.05mol/l soaks, and desorbs Cd, Pb, Zn tri-heavy metal species of filling adsorption, finally rushes with tap water Wash, the repeatable utilization of filler after drying.

Claims (5)

1. a plant bionic and the method for microbial association repairing polluted soil, it is characterised in that comprise the following steps:
(1) level land, investigation soil pollution situation and the spatial distribution of pollutant thereof;
(2) determine the concentration of target contaminant, determine recovery scenario according to the character of pollutant, concentration, Soil Utilization type, bag Include kind and the distribution scheme of the microorganism determining the distribution of plant bionic prosthetic device, the scheme of classification and needs;
(3) select the filler for target contaminant with enrichment, make plant bionic prosthetic device, according to step (2) The scheme about plant bionic prosthetic device of repairing in target carries out the plant bionic reparation of contaminated soil;
(4) during step (3) plant bionic prosthetic device is repaired, screening can convert or degrade target stains The microorganism of thing or add inoculating microbe, repairs in target about the technical scheme of microorganism at soil according to step (2) In again arrange add microorganism, change the existing forms of metal in soil, degradable organic pollutant matter, microorganism with plant imitate Raw recovery technique is used in combination, and improves soil remediation efficiency;
(5) plant bionic prosthetic device and the recycling of filler.
2., according to the method for a kind of plant bionic described in claim 1 Yu microbial association repairing polluted soil, its feature exists In, described step (2) determines concentration and the character thereof of polluter, determines the distribution density of plant bionic prosthetic device, insert Enter the degree of depth of soil.
3., according to the method for a kind of plant bionic described in claim 1 Yu microbial association repairing polluted soil, its feature exists In, in step (3), plant bionic repairs the selection requirement of filler can be enriched with this target stains material, is fixed by polluter Among filler, require that polluter can by acid, alkali or thermal desorption out so that filler can recycle simultaneously.
4., according to the method for a kind of plant bionic described in claim 1 Yu microbial association repairing polluted soil, its feature exists In, in described step (4), the addition opportunity of step (4) microorganism and addition are typically based on reparation final goal and can add at any time Enter.
5., according to the method for a kind of plant bionic described in claim 1 Yu microbial association repairing polluted soil, its feature exists It is chosen to make polluted heavy metals in target contaminant be changed into the dissolved by combined state form or/and increase in, microorganism Add the dissolubility of soil organic pollutant matter in target contaminant.
CN201610842846.9A 2016-09-22 2016-09-22 A kind of plant bionic and the method for microbial association repairing polluted soil Pending CN106269825A (en)

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Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107199240A (en) * 2017-06-02 2017-09-26 云南省环境科学研究院(中国昆明高原湖泊国际研究中心) A kind of method for administering soil pollution and preparation method thereof
CN109317513A (en) * 2018-10-22 2019-02-12 苏州金螳螂园林绿化景观有限公司 Northern river low-lying land soil pollution repair system
CN110357353A (en) * 2019-07-11 2019-10-22 广东雅迪环保设备有限公司 A kind of refuse landfill recharge system of imitation biochemistry
CN113240282A (en) * 2021-05-14 2021-08-10 北京市环境保护科学研究院 Method for determining mercury contaminated soil remediation standard through multi-target layering
CN114951258A (en) * 2022-05-30 2022-08-30 四川省自然资源科学研究院(四川省生产力促进中心) Method for restoring soil by combining plant extract with EK-PRB

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CN103736719A (en) * 2014-01-02 2014-04-23 重庆绿色智能技术研究院 In-situ self-sustaining contaminated soil restoring device based on plant bionics and method
CN103736721A (en) * 2014-01-23 2014-04-23 雷学军 Method for biologically remediating water body and soil comprehensively utilizing resources
CN104190704A (en) * 2014-08-19 2014-12-10 山西省环境科学研究院 Method for remedying soil in sewage irrigation area
CN105149332A (en) * 2015-09-30 2015-12-16 河南行知专利服务有限公司 Method for remediating heavy metal contaminated soil through combination of microorganism and plant

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101954373A (en) * 2010-09-21 2011-01-26 上海大学 Method for restoring oil polluted wet land by combination of plant and microorganism
CN103736719A (en) * 2014-01-02 2014-04-23 重庆绿色智能技术研究院 In-situ self-sustaining contaminated soil restoring device based on plant bionics and method
CN103736721A (en) * 2014-01-23 2014-04-23 雷学军 Method for biologically remediating water body and soil comprehensively utilizing resources
CN104190704A (en) * 2014-08-19 2014-12-10 山西省环境科学研究院 Method for remedying soil in sewage irrigation area
CN105149332A (en) * 2015-09-30 2015-12-16 河南行知专利服务有限公司 Method for remediating heavy metal contaminated soil through combination of microorganism and plant

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107199240A (en) * 2017-06-02 2017-09-26 云南省环境科学研究院(中国昆明高原湖泊国际研究中心) A kind of method for administering soil pollution and preparation method thereof
CN109317513A (en) * 2018-10-22 2019-02-12 苏州金螳螂园林绿化景观有限公司 Northern river low-lying land soil pollution repair system
CN110357353A (en) * 2019-07-11 2019-10-22 广东雅迪环保设备有限公司 A kind of refuse landfill recharge system of imitation biochemistry
CN113240282A (en) * 2021-05-14 2021-08-10 北京市环境保护科学研究院 Method for determining mercury contaminated soil remediation standard through multi-target layering
CN113240282B (en) * 2021-05-14 2023-05-09 北京市环境保护科学研究院 Method for multi-target layering determination of mercury contaminated soil restoration standard
CN114951258A (en) * 2022-05-30 2022-08-30 四川省自然资源科学研究院(四川省生产力促进中心) Method for restoring soil by combining plant extract with EK-PRB

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Application publication date: 20170104