CN109332376A - A kind of restorative procedure for soil pollution by heavy metal - Google Patents
A kind of restorative procedure for soil pollution by heavy metal Download PDFInfo
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- CN109332376A CN109332376A CN201811216890.4A CN201811216890A CN109332376A CN 109332376 A CN109332376 A CN 109332376A CN 201811216890 A CN201811216890 A CN 201811216890A CN 109332376 A CN109332376 A CN 109332376A
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
- B09C1/105—Reclamation of contaminated soil microbiologically, biologically or by using enzymes using fungi or plants
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/02—Extraction using liquids, e.g. washing, leaching, flotation
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/08—Reclamation of contaminated soil chemically
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C2101/00—In situ
Abstract
The invention discloses a kind of restorative procedures for soil pollution by heavy metal, comprising: (1) magnetotactic bacteria fluid nutrient medium is added in the soil of heavy metal pollution, is inoculated with magnetotactic bacteria, turns over；(2) magnetotactic bacteria fluid nutrient medium is added in heavy-metal contaminated soil, and magnetic field is set；(3) surface layer of heavy-metal contaminated soil is collected；(4) heavy-metal contaminated soil that elution is collected；(5) soil conditioner is spread fertilizer over the fields on heavy-metal contaminated soil surface；(6) the super accumulation crop of heavy metal is planted in heavy-metal contaminated soil；(7) after crop kernel is formed, heavy metal barrier is sprayed；(8) circulating repetition executes step (1)-step (7) 2-3 times.The present invention has many advantages, such as that plantation difficulty is low, is easily managed, is suitable for pollution in wide area soil remediation, is few to environmental perturbation, and soil remediation effect is good, and can beautify the environment while pollution administration soil, and replicability is strong.
Description
Technical field
The present invention relates to contaminated soil Treatment process field, specially a kind of reparation side for soil pollution by heavy metal
Method.
Background technique
Soil be not only earth ecology circle important component and important natural resources for the survival of mankind it
One.With the rural activities such as the industrial activities such as exploitation of mineral resources, smelting and sewage irrigation, applying sludge and chemical fertilizer inferior into
Row, the harmful heavy metals such as Cd, Pb constantly enter in soil environment, pollute to normal production and living.Therefore, soil is administered
Heavy metal pollution to conservation culture set and the life of human health is of great significance.
The method that the traditional improvement restorative procedure of heavy metal pollution of soil is usually physics and chemistry, such as dilution and earthing
Method, vitrification, heat treatment technics, elution method, electrochemical process etc. last although traditional restorative procedure regulation effect is good
It is shorter, but all there are many defects.Such as traditional administering method it is at high cost, not manageability, easily cause secondary pollution etc..
Phytoremediation technology refers to using plant and its rhizosphere microorganism to the absorption of soil pollutant, volatilization, conversion, drop
Solution, fixed function and remove the recovery technique of pollutant in soil.Have now been found that many plants can be simultaneously to an a variety of huge sum of moneys
Category has a powerful accumulation ability, and heavy metal mainly includes Hg, Cd, Pb, Cr and metalloid As etc., and the heavy metal in environment can be with
It is absorbed by plants, if human health will be endangered into plant edible part.To avoid the exposure to human body, in recent years
The research for selecting non-consumption commercial crop plants to carry out phytoremediation increasingly causes to pay close attention to, but most of reported plants are repaired
Compound method effect is significant, slow effect, repairing effect are unstable, along with being limited by weather, soil environment, so that these
The Accumulation of heavy metals amount of plant is limited, to above be restricted in its application.
Microorganism remediation is that have absorption, precipitating, oxidation and reduction etc. to heavy metal using the certain micro-organisms in soil
Effect, to reduce the toxicity of heavy metal in soil.The technology can be such that heavy metal contaminants remove from soil, to ring around
Border influence is smaller, will not generate secondary pollution, the huge soil remediation potentiality having.But existing heavy-metal contaminated soil is micro-
Bioremediation technology has some limitations, and such as microorganism remediation duration length, slow effect generally form complex compound reduction
The harm of heavy metal can not remove heavy metal from soil.
As described above, being badly in need of solving following technical problem to preferably administer heavy metal metal contaminated soil:
1. determine suitable tolerable crop, have plantation difficulty is low, be easily managed, be suitable for pollution in wide area soil,
The feature that growth is rapid, biomass is big.
2. overcoming the Metal uptake inefficiency of tolerable crop, heavy metal to repair, element is single, repair time is too long, deposits
The low problem of motility rate.
3. the case where changing during avoiding heavy-metal contaminated soil repairing and treating and destroying soil texture.
Summary of the invention
The purpose of the present invention is to provide a kind of restorative procedures for soil pollution by heavy metal, to solve above-mentioned background skill
The problem of being proposed in art.
In order to solve the above technical problem, the present invention provides following technical solutions: a kind of for soil pollution by heavy metal
Restorative procedure, specifically includes the following steps:
(1), magnetotactic bacteria fluid nutrient medium is added in the soil of heavy metal pollution, is inoculated with magnetotactic bacteria, turn over weight
The soil of the surface layer thickness 10-12cm of metallic pollution soil, fills magnetotactic bacteria culture medium, magnetotactic bacteria and heavy-metal contaminated soil
Divide mixing；
(2), it cultivates 45-50 days, the training of magnetotactic bacteria liquid is added in the soil of heavy-metal contaminated soil surface layer 3-5cm thickness
Base is supported, and is irrigated, so that heavy-metal contaminated soil is kept waterflooding state, and magnetic is set in the soil top of the heavy metal pollution
, it is kept for 29-34 days；
(3), the soil of heavy-metal contaminated soil surface layer thickness 4-6cm described in collection step (2)；
(4), with the heavy-metal contaminated soil collected in water rinsing step (3), make magnetotactic bacteria and heavy-metal contaminated soil
It is segregated into aqueous solution, repeats elution 2-4 times, then heavy metallic activation bacterium bacterium solution is inoculated into the soil cleaned, it will
The soil fills out go back to original place；
(5), soil conditioner is uniformly spread fertilizer over the fields in step (4) heavy-metal contaminated soil surface, with a thickness of 1-4cm, wherein soil
The dosage of earth conditioner is the 5%-10% of soil quality, then spray sprinkler, and the quality of water used in spray sprinkler is soil tune
The 60%-80% for managing agent quality turns over uniformly, stands 5-24h, again spray sprinkler, and water consumption is the 80%- of soil quality
100%, finally soil is placed under natural environment, avoids heavy rain from washing away in the first two months；The soil conditioner raw material is pressed
Weight percent meter, comprising: red mud 20-30%, rape straw 20-30%, dolomite dust 5-15%, pulverized limestone 25-35%,
Silicon-calcium-potafertilizer fertilizer 20-30%, amino acid 1-10%；
(6), the super accumulation crop of heavy metal, in due course Soil management measure are planted in step (5) heavy-metal contaminated soil；
(7), after crop kernel is formed, heavy metal barrier is sprayed, is harvested to crop；
Wherein, the heavy metal barrier is liquid solution, composition Na2SeO3, selenium content 40mg/L；
The configuration method of the heavy metal barrier are as follows: weigh Na per acre2SeO34.38g, amount of water 50L, dilution stirring are equal
It is even to get heavy metal barrier；
The heavy metal barrier spraying method are as follows: crop kernel sprays after being formed, and sprays frequency as the sprinkling of per two weeks
Once, continue 1-2 months；
(8), circulating repetition executes step (1)-step (7), until heavy metal in soil content reaches safety standard.
Further, the inoculative proportion of magnetotactic bacteria described in step (1) is the soil of every weight per square meter metallic pollution
Middle inoculation 9.0 × 109-1.0×1010A magnetotactic bacteria.
Further, fluid nutrient medium additive amount described in step (1) is that every square metre of heavy-metal contaminated soil sprays
50-80ml fluid nutrient medium；
Fluid nutrient medium additive amount described in step (2) is that every square metre of heavy-metal contaminated soil sprays 30-50ml liquid
Body culture medium.
Further, magnetic direction faces upward with deviating from step (2), and magnetic field strength is 200-300 Gauss.
Further, heavy metallic activation bacterium bacterium solution includes bacillus and Pseudomonas fluorescens in step (4).
Further, the bacillus bacterium solution and Pseudomonas fluorescens bacterium solution volume ratio are 1-2:1, and every kilogram eluted
Soil inoculation heavy metallic activation bacterium solution 100-300mL.
Further, it after the super accumulation crop of the plantation heavy metal, is applied into heavy-metal contaminated soil within every 5-7 months
Fertilizer containing N, P, K is as top dressing.
Further, further include the curing schedule executed after step (7): spraying to heavy-metal contaminated soil can chelating
The adsorbent of free metal, the adsorbent is humic acid, and increases in the humic acid and have lime.
Further, the super accumulation crop of the heavy metal planted in step (5) is one of a-f below or a variety of:
A: pulse family class over-accumulated plant；
B: non-pulse family class over-accumulated plant；
C: short over-accumulated plant；
D: non-short over-accumulated plant；
E: negative over-accumulated plant is liked；
F: positive over-accumulated plant is liked.
Further, the pulse family class over-accumulated plant is wild cowpea, long wave leaf beggarweed or clover；
The short over-accumulated plant is fragrant eupatorium herb, Sedum alfredii Hance, green water cress, water spinach or ciliate desert-grass；
The non-short over-accumulated plant is Leersia Sw, elsholtzia splendens, canna or safflower；
The negative over-accumulated plant of the happiness is Sedum alfredii Hance or ciliate desert-grass；
The positive over-accumulated plant of the happiness is to spend more sunflower.
Compared with prior art, the beneficial effects obtained by the present invention are as follows being:
(1), magnetotactic bacteria belongs to Gram-negative bacteria, is obligate micro- aerobic, facultative or obligate anaerobes, has and bears
Oxygen characteristic has into the cell along the arrangement of thallus long axis, by the coated magnetic corpusculum particle chain of film, can orient fortune under the influence of a magnetic field
It is dynamic, and have stronger adsorption capacity to heavy metal；Magnetotactic bacteria can be mobile to deep under ground under the action of earth's magnetic field, by
Active force in earth's magnetic field is very weak, magnetic field is arranged in soil surface, and the direction by controlling magnetic field concentrates on magnetotactic bacteria
In small area, the purpose that heavy-metal contaminated soil is irrigated in step (2) is to reduce the oxygen content of upper soll layer, and creation is conducive to become
The environment of magnetic bacteria live.
(2), magnetotactic bacteria is based on the absorption with cell surface to the absorption of heavy metal ion, therefore magnetotactic bacteria counterweight
The adsorption rate of metal quickly, effectively adsorbs the heavy metal in soil during its movement, greatly reduces weight in soil
The accumulation of metal.
(3), leacheate obtained in step (4) recycles heavy metal, on the one hand prevents heavy metal from returning to soil or water
Body, on the other hand the heavy metal of recycling has very high surcharge, can be reached with additional income, the rate of recovery of magnetotactic bacteria
90% or more, leacheate heavy metal recovery rate reaches 99% or more, is conducive to the reparation of heavy-metal contaminated soil.
(4), bacillus and Pseudomonas fluorescens can generate the organic acid of a variety of low molecular weights, such as first by metabolism
Acid, acetic acid, propionic acid and butyric acid etc., so that the direct or indirect form for affecting heavy metal in soil, plays activation heavy metal
Effect, improve plant to the absorption efficiency of heavy metal.
(5), heavy metal-passivated using soil conditioner, super accumulation crop barrier heavy metal, impedance agent resistance control heavy metal etc.
Three-level measure hinders control heavy metal-polluted soil in farmland production overall process and enters agricultural product, and more single resistance control measure is more secure, drop
Low heavy metal pollutes agricultural product risk.
(6), the present invention has implemented the thought of waste utilization and circular economy, using industrial waste red mud and agricultural wastes
Stalk is conditioner raw material, can consume a large amount of solid waste.
(7), the heavy metal resistance control agent raw material that the present invention selects is selenium salt, while heavy metal is controlled in resistance, is conducive to improve
Se content in crop kernel improves quality of agricultural product.
(8), it is promoted on arable layer A by the collocation and interplanting of different type plant to enhance the diversity of biological sample kind
Ecological environment stability, the growth for plant of mutually promoting；Specifically, short and the interplanting of non-short are conducive to space
It makes full use of；Pulse family class and the interplanting of non-pulse family class are conducive to realize that nutrient is self-supporting by fixed nitrogen；Happiness male form and happiness female interplanting have
It is acted on conducive to making full use of illumination and reaching protection ombrophyte.
As stated above, the present invention accomplishes that farmland is raw with three kinds of passivation, plant barrier, agronomy regulation recovery technique combinations
It produces overall process resistance control heavy metal and enters food chain, reduce heavy metal activity, reduce soil heavy metal cadmium activity, resistance control heavy metal cadmium
Into agricultural product seed, quality of agricultural product is improved, while having that small investment, technical requirements be not high, plantation difficulty is low, is easy to pipe
The advantages that reason is suitable for pollution in wide area soil remediation, is few to environmental perturbation, and soil remediation effect is good, and dirt can administered
It beautifies the environment while contaminating soil, replicability is strong.
Specific embodiment
It is clearly and completely described below in conjunction with the technical solution in the present invention, it is clear that described embodiment is only
It is only a part of the embodiment of the present invention, instead of all the embodiments.Based on the embodiments of the present invention, ordinary skill
Personnel's every other embodiment obtained without making creative work, shall fall within the protection scope of the present invention.
The extracting method of magnetotactic bacteria: by the activated sludge of sewage treatment plant with have been subjected to sterilizing be down to room temperature enrichment train
It supports base to be uniformly mixed by the volume ratio of l:2, is put into wide-mouth bottle, seals bottleneck with sterilized gauze, 28 DEG C of standings are protected from light training
Support 30d, by the magnetotactic bacteria of collection according to 10% inoculum concentration, be inoculated into enriched medium, it is static be protected from light, at 23 DEG C, work as bacterium
Liquid concentration reaches 9.0 × 109-1.0×1010When, the performance of measurement absorption heavy metal is specific as follows:
Bacterium solution 10ml is taken, the Ni of 100ml is added to3+Ion concentration 30mg/L, Fe3+Ion concentration 50mg/L, Pb2+Ion contains
Measure 50mg/L, Cu3+2h is cultivated in the solution of ion concentration 40mg/L at 28 DEG C, measures Ni in water3+、Pb2+、Cu3+And Fe3+Ion
Content, Ni3+、Pb2+、Cu3+And Fe3+Ionic adsorption rate respectively reaches 80% or more, i.e., makes as magnetotactic bacteria of the invention
With.Magnetotactic bacteria in the present invention can be separated from other activated sludge or the soil of heavy metal pollution, can also make be
Existing disclosed magnetotactic bacteria bacterial strain, as long as its adsorption rate obtains requirement.Used magnetotactic in following embodiment is thin
Bacterium screens according to the method described above, repeats no more.
The fluid nutrient medium (enriched medium) of magnetotactic bacteria forms: 4000mg/L CHOHO, 1500mg/L (NH4)
SO4, 200mg/L CH3COONa, 1500mg/L Fe2(SO4)3, 50mg/L cobalamin, 2ml/L ironic citrate, 30mg/L
MgSO4, 10mg/L ZnSO4, 10mg/L MnSO4。
The culture of bacillus and Pseudomonas fluorescens: bacillus and Pseudomonas fluorescens is taken to be inoculated into liquid training respectively
It supports in base, according to every milliliters of liquid inoculation of medium 1 × 106-2.5×107A bacterium, the pH of fluid nutrient medium are 6-7, training
Supporting temperature is 32-36 DEG C, after cultivating 15-20h, obtains the bacterium solution in growth logarithmic phase, in every milliliter of culture solution containing 5.0 ×
107-3.5×108Then a bacterium mixes bacillus bacterium solution with Pseudomonas fluorescens culture bacterium solution according to volume ratio 1-2:1
It closes, both obtains heavy metallic activation bacterium bacterium solution.
The fluid nutrient medium of bacillus and Pseudomonas fluorescens: glucose containing 20g, 15g peptone, 5g chlorine in every 1L water
Change sodium and 0.5g beef extract.
A plurality of types of heavy metal over-accumulated plants of composite plant on arable layer A, such as a-f below:
A. pulse family class over-accumulated plant: wild cowpea, long wave leaf beggarweed or clover；
B. all over-accumulated plants in addition to a；
C. short over-accumulated plant: fragrant eupatorium herb, Sedum alfredii Hance, green water cress, water spinach, ciliate desert-grass；
D. non-short over-accumulated plant: Leersia Sw, elsholtzia splendens, canna, safflower；
E. negative over-accumulated plant: Sedum alfredii Hance, ciliate desert-grass is liked；
F. it likes positive over-accumulated plant: spending more sunflower.
The life on arable layer A is promoted by the collocation and interplanting of different type plant to enhance the diversity of biological sample kind
The stability of state environment, the growth for plant of mutually promoting.Specifically, short is conducive to the abundant of space with the interplanting of non-short
It utilizes；Pulse family class and the interplanting of non-pulse family class are conducive to realize that nutrient is self-supporting by fixed nitrogen；Happiness male form and happiness female interplanting are conducive to
It makes full use of illumination and reaches protection ombrophyte effect.
Further, a small amount of lime is added in humic acid, is formed the modifying agent that pH value is 7.5-9, is promoted using lime
It is acted on into complexing chelating of the humic acid to heavy metal, enhances heavy metals immobilization effect.
It is 30cm, area 100m by contaminated by heavy metals thickness of soil2Soil as experimental field, and by monolith reality
It tests field and carries out sample prescription division, length and width are 5m, and it is the ridge of 0.4m that width is stayed between each sample prescription, and each sample prescription is according to diagonal line principle
6 sampled points are set.The soil of experimental plot surface layer 0-30cm is collected, shady and cool ventilation dries, and removes impurity.Measuring soil pH is
6.3, the content of heavy metal Pb, Ni, Cu and Fe in the soil is respectively: the concentration of Pb element is 595mg/kg-1, Ni element it is dense
Degree is 382mg/kg-1, the concentration of Cu element is 432mg/kg-1, the concentration of Fe element is 583mg/kg-1。
According to national standard of soil environment quality GB15618-2008 it is found that in the case where soil pH=5.5-6.5, agricultural
Land used nonirrigated farmland heavy metal Pb, Ni and Cu secondary standard value are respectively 80,80 and 50mg/kg, and the average value of Fe is in soil
297mg/kg；Therefore, there are more serious heavy metal pollutions for above-mentioned monolith experimental field.
Embodiment 1:
A kind of restorative procedure for soil pollution by heavy metal, specifically includes the following steps:
(1), magnetotactic bacteria culture medium is added in the soil of heavy metal pollution, magnetotactic bacteria culture medium adding proportion is
50mL magnetotactic bacteria fluid nutrient medium is added in every square metre of heavy-metal contaminated soil, and in the soil of every weight per square meter metallic pollution
9.0 × 10 are inoculated in earth9A magnetotactic bacteria turns over the soil of the surface layer thickness 10cm of heavy-metal contaminated soil, trains magnetotactic bacteria
Base, magnetotactic bacteria and heavy-metal contaminated soil is supported to be sufficiently mixed；
(2), it cultivates 45 days, magnetotactic bacteria culture medium, addition is added in the soil of heavy-metal contaminated soil surface layer 3cm thickness
Ratio is 30mL magnetotactic bacteria fluid nutrient medium to be added in every square metre of heavy-metal contaminated soil, and irrigate the soil, makes a huge sum of money
Belong to contaminated soil and keep waterflooding state, and forms magnetic field, magnetic direction in the soil top of heavy metal pollution setting hot-wire coil
It faces upward away from ground, magnetic field strength is 200 Gausses, is kept for 29 days；
(3), the soil of collection step (2) heavy-metal contaminated soil surface layer thickness 4cm；
(4), with the heavy-metal contaminated soil collected in water rinsing step (3), elution 2 times is repeated, then by bacillus
It is inoculated into the soil cleaned with Pseudomonas fluorescens, the volume ratio 1:1 of bacillus bacterium solution and Pseudomonas fluorescens bacterium solution,
Every kilogram eluted the mixed bacterium solution 100mL of soil inoculation, point 3 inoculations, inoculated bacillus and Pseudomonas
The soil of bacterium fills out go back to original place；
(5), soil conditioner is uniformly spread fertilizer over the fields in step (4) heavy-metal contaminated soil surface, with a thickness of 2cm, wherein soil
The dosage of conditioner is the 7% of soil quality, then spray sprinkler, and the quality of water used in spray sprinkler is soil conditioner quality
68%, turn over uniformly, stand 11h, again spray sprinkler, water consumption is the 88% of soil quality, finally by soil be placed in from
Under right environment, heavy rain is avoided to wash away in the first two months；The soil conditioner raw material is by weight percentage, comprising: red mud
24%, rape straw 24%, dolomite dust 9%, pulverized limestone 29%, silicon-calcium-potafertilizer fertilizer 24% and amino acid 4%；
(6), the super accumulation crop of heavy metal, in due course Soil management measure are planted in step (5) heavy-metal contaminated soil；
(7), after crop kernel is formed, heavy metal barrier is sprayed, is harvested to crop；
Wherein, the heavy metal barrier is liquid solution, composition Na2SeO3, selenium content 40mg/L；
The configuration method of the heavy metal barrier are as follows: weigh Na2SeO34.38g, amount of water 50L, dilution stirring per acre
Uniformly to get heavy metal barrier；
The heavy metal barrier spraying method are as follows: crop kernel sprays after being formed, and sprays frequency as the sprinkling of per two weeks
Once, continue 1 month；
(9), circulating repetition executes step (1)-step (7) 2 times.
As the result is shown: be compared to native soil, soil pH 5.9, heavy metal Pb, Ni, Cu and Fe concentration be respectively
57.15, the concentration of 61.35,51.37 and 254.18mg/kg, heavy metal in soil Pb, Ni, Cu and Fe have dropped respectively
90.40%, 84.03%, 88.11% and 56.40%.
Embodiment 2:
(1), magnetotactic bacteria culture medium is added in the soil of heavy metal pollution, magnetotactic bacteria culture medium adding proportion is
80mL magnetotactic bacteria fluid nutrient medium is added in every square metre of heavy-metal contaminated soil, and in the soil of every weight per square meter metallic pollution
1.0 × 10 are inoculated in earth10A magnetotactic bacteria turns over the soil of the surface layer thickness 12cm of heavy-metal contaminated soil, trains magnetotactic bacteria
Base, magnetotactic bacteria and heavy-metal contaminated soil is supported to be sufficiently mixed；
(2), it cultivates 50 days, magnetotactic bacteria culture medium, addition is added in the soil of heavy-metal contaminated soil surface layer 5cm thickness
Ratio is 50mL magnetotactic bacteria fluid nutrient medium to be added in every square metre of heavy-metal contaminated soil, and irrigate the soil, makes a huge sum of money
Belong to contaminated soil and keep waterflooding state, and forms magnetic field, magnetic direction in the soil top of heavy metal pollution setting hot-wire coil
It faces upward away from ground, magnetic field strength is 265 Gausses, is kept for 34 days；
(3), in collection step (2) heavy-metal contaminated soil surface layer thickness 6cm soil；
(4), with the heavy-metal contaminated soil collected in water rinsing step (3), elution 4 times is repeated, then by bacillus
It is inoculated into the soil cleaned with Pseudomonas fluorescens, the volume ratio 2:1 of bacillus bacterium solution and Pseudomonas fluorescens bacterium solution,
Every kilogram eluted the mixed 150mL of soil inoculation, points of 5 times inoculations, inoculated bacillus and Pseudomonas fluorescens
Soil fills out go back to original place；
(5), soil conditioner is uniformly spread fertilizer over the fields in step (4) heavy-metal contaminated soil surface, with a thickness of 4cm, wherein soil
The dosage of conditioner is the 10% of soil quality, then spray sprinkler, and the quality of water used in spray sprinkler is soil conditioner matter
The 80% of amount is turned over uniformly, is stood for 24 hours, again spray sprinkler, and water consumption is the 100% of soil quality, is finally placed in soil
Under natural environment, heavy rain is avoided to wash away in the first two months；The soil conditioner raw material is by weight percentage, comprising: red
Mud 30%, rape straw 30%, dolomite dust 5%, pulverized limestone 35%, silicon-calcium-potafertilizer fertilizer 30% and amino acid 1 0%；
(6), the super accumulation crop of heavy metal, in due course Soil management measure are planted in step (5) heavy-metal contaminated soil；
(7), after crop kernel is formed, heavy metal barrier is sprayed, is harvested to crop；
Wherein, the heavy metal barrier is liquid solution, composition Na2SeO3, selenium content 40mg/L；
The configuration method of the heavy metal barrier are as follows: weigh Na2SeO34.38g, amount of water 50L, dilution stirring per acre
Uniformly to get heavy metal barrier；
The heavy metal barrier spraying method are as follows: crop kernel sprays after being formed, and sprays frequency as the sprinkling of per two weeks
Once, continue 2 months；
(8), circulating repetition executes step (1)-step (7) 3 times.
As the result is shown: be compared to native soil, soil pH 5.3, heavy metal Pb, Ni, Cu and Fe concentration be respectively
47.52, the concentration of 59.48,32.21 and 132.07mg/kg, heavy metal in soil Pb, Ni, Cu and Fe have dropped respectively
92.01%, 84.42%, 92.54% and 77.35%.
As stated above, native after the reparation of 2-3 using the restorative procedure of soil pollution by heavy metal of the present invention
The content of heavy metal in national standard of soil environment quality secondary standard value hereinafter, can be completed in a short period of time in earth
Reparation to heavy metal-polluted soil.
Finally, it should be noted that the foregoing is only a preferred embodiment of the present invention, it is not intended to restrict the invention,
Although the present invention is described in detail referring to the foregoing embodiments, for those skilled in the art, still may be used
To modify the technical solutions described in the foregoing embodiments or equivalent replacement of some of the technical features.
All within the spirits and principles of the present invention, any modification, equivalent replacement, improvement and so on should be included in of the invention
Within protection scope.
Claims (10)
1. a kind of restorative procedure for soil pollution by heavy metal, it is characterised in that: specifically includes the following steps:
(1), magnetotactic bacteria fluid nutrient medium is added in the soil of heavy metal pollution, is inoculated with magnetotactic bacteria, turns over heavy metal
The soil of the surface layer thickness 10-12cm of contaminated soil keeps magnetotactic bacteria culture medium, magnetotactic bacteria and heavy-metal contaminated soil sufficiently mixed
It closes；
(2), it cultivates 45-50 days, magnetotactic bacteria Liquid Culture is added in the soil of heavy-metal contaminated soil surface layer 3-5cm thickness
Base, and irrigate, so that heavy-metal contaminated soil is kept waterflooding state, and magnetic field is set in the soil top of the heavy metal pollution,
It is kept for 29-34 days；
(3), the soil of heavy-metal contaminated soil surface layer thickness 4-6cm described in collection step (2)；
(4), with the heavy-metal contaminated soil collected in water rinsing step (3), separate magnetotactic bacteria with heavy-metal contaminated soil
Into in aqueous solution, elution 2-4 times is repeated, then heavy metallic activation bacterium bacterium solution is inoculated into the soil cleaned, it will be described
Soil fills out go back to original place；
(5), soil conditioner is uniformly spread fertilizer over the fields in step (4) heavy-metal contaminated soil surface, with a thickness of 1-4cm, wherein soil tune
The dosage for managing agent is the 5%-10% of soil quality, then spray sprinkler, and the quality of water used in spray sprinkler is soil conditioner
The 60%-80% of quality turns over uniformly, stands 5-24h, again spray sprinkler, and water consumption is the 80%-100% of soil quality,
Finally soil is placed under natural environment, avoids heavy rain from washing away in the first two months；The soil conditioner raw material by weight hundred
Divide than meter, comprising: red mud 20-30%, rape straw 20-30%, dolomite dust 5-15%, pulverized limestone 25-35%, silico-calcium potassium
Fertile 20-30%, amino acid 1-10%；
(6), the super accumulation crop of heavy metal, in due course Soil management measure are planted in step (5) heavy-metal contaminated soil；
(7), after crop kernel is formed, heavy metal barrier is sprayed, is harvested to crop；
Wherein, the heavy metal barrier is liquid solution, composition Na2SeO3, selenium content 40mg/L；
The configuration method of the heavy metal barrier are as follows: Na2SeO34.38g, amount of water 50L are weighed per acre, dilution stirs evenly,
Up to heavy metal barrier；
The heavy metal barrier spraying method are as follows: crop kernel sprays after being formed, and spraying frequency is that the sprinkling of per two weeks is primary,
Continue 1-2 months；
(8), circulating repetition executes step (1)-step (7), until heavy metal in soil content reaches safety standard.
2. a kind of restorative procedure for soil pollution by heavy metal according to claim 1, it is characterised in that: step (1)
Described in magnetotactic bacteria inoculative proportion be every weight per square meter metallic pollution soil in be inoculated with 9.0 × 109-1.0×1010It is a
Magnetotactic bacteria.
3. a kind of restorative procedure for soil pollution by heavy metal according to claim 1, it is characterised in that: step (1)
Described in fluid nutrient medium additive amount be every square metre of heavy-metal contaminated soil spray 50-80ml fluid nutrient medium；
Fluid nutrient medium additive amount described in step (2) is that every square metre of heavy-metal contaminated soil sprays the training of 30-50ml liquid
Support base.
4. a kind of restorative procedure for soil pollution by heavy metal according to claim 1, it is characterised in that: step (2)
Middle magnetic direction faces upward with deviating from, and magnetic field strength is 200-300 Gauss.
5. a kind of restorative procedure for soil pollution by heavy metal according to claim 1, it is characterised in that: step (4)
Middle heavy metallic activation bacterium bacterium solution includes bacillus and Pseudomonas fluorescens.
6. a kind of restorative procedure for soil pollution by heavy metal according to claim 5, it is characterised in that: the gemma
Bacillus bacterium solution and Pseudomonas fluorescens bacterium solution volume ratio are 1-2:1, and every kilogram eluted soil inoculation heavy metallic activation bacterium solution
100-300mL。
7. a kind of restorative procedure for soil pollution by heavy metal according to claim 1, it is characterised in that: the plantation
After the super accumulation crop of heavy metal, the fertilizer containing N, P, K is applied into heavy-metal contaminated soil as top dressing within every 5-7 months.
8. a kind of restorative procedure for soil pollution by heavy metal according to claim 1, it is characterised in that: further include
The curing schedule executed after step (7): the adsorbent of energy chelating free metal, the suction are sprayed to heavy-metal contaminated soil
Attached dose is humic acid, and increases in the humic acid and have lime.
9. a kind of restorative procedure for soil pollution by heavy metal according to claim 1, it is characterised in that: step (5)
The super accumulation crop of middle planted heavy metal is one of a-f below or a variety of:
A: pulse family class over-accumulated plant；
B: non-pulse family class over-accumulated plant；
C: short over-accumulated plant；
D: non-short over-accumulated plant；
E: negative over-accumulated plant is liked；
F: positive over-accumulated plant is liked.
10. a kind of restorative procedure for soil pollution by heavy metal according to claim 9, it is characterised in that:
The pulse family class over-accumulated plant is wild cowpea, long wave leaf beggarweed or clover；
The short over-accumulated plant is fragrant eupatorium herb, Sedum alfredii Hance, green water cress, water spinach or ciliate desert-grass；
The non-short over-accumulated plant is Leersia Sw, elsholtzia splendens, canna or safflower；
The negative over-accumulated plant of the happiness is Sedum alfredii Hance or ciliate desert-grass；
The positive over-accumulated plant of the happiness is to spend more sunflower.
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