CN106001097B - A method of joint repairs DDTs-PAHs combined contamination soil - Google Patents
A method of joint repairs DDTs-PAHs combined contamination soil Download PDFInfo
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- CN106001097B CN106001097B CN201610241718.9A CN201610241718A CN106001097B CN 106001097 B CN106001097 B CN 106001097B CN 201610241718 A CN201610241718 A CN 201610241718A CN 106001097 B CN106001097 B CN 106001097B
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/08—Reclamation of contaminated soil chemically
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
- B09C1/105—Reclamation of contaminated soil microbiologically, biologically or by using enzymes using fungi or plants
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Abstract
A method of joint repairs DDTs-PAHs combined contamination soil, it is related to a kind of method of repairing polluted soil, is strengthened using rhanolipid as biosurfactant combined highly effective mixed degradation bacterium and remaining organo-chlorine pesticide DDT and polycyclic aromatic hydrocarbon in facility agricultural soil is promoted to be decomposed, converted；Low accumulation type vegetable crop is planted simultaneously, it is metabolized by root system of plant and the chemical stimulation of root rhizosphere secreta acts on further enhancement microbiological growth and the degradation to organic pollutant DDT and polycyclic aromatic hydrocarbon, this method has small to environmental disruption, do not generate secondary pollution, it is low-cost, be easy to operate in large scale, do not influence production and the advantages of income, it is the based technique for in-situ remediation that one kind is safe and effective, can produce when repairing, is suitble to still in the middle low pollution facility agricultural soil reparation of long-term cropping.
Description
Technical field
The present invention relates to the research field of agricultural soil organic contamination reparation, specifically a kind of restoration facilities agricultural soil
The surfactant of organo-chlorine pesticide DDT and polycyclic aromatic hydrocarbon composite pollution-plant-microorganism combined remediation method in earth.
Background technique
All the time, in order to pursue higher yield and economic benefit, the sowing amount of fertilizer and pesticide is continuously increased people.
According to statistics, the annual applications of pesticide amount in China is up to 50~60 × 107 kg.During pesticide spraying, only 8% pesticide can be by crop
It absorbs, about 40~50% pesticide can fall in upper soll layer.The whole nation is reached 16,000,000 hm by the farmland of pollution by pesticides2, mainly
The pesticide exceeding standard rate of agricultural product is up to 16-20%.Although having stopped since implementation agriculture chemical registration system at the beginning of the last century 80's
Only produce and use the pesticides such as DDT, Niran, heptachlor, but due to China between 60~eighties of 20th century once mass production
With use organo-chlorine pesticide, a large amount of pesticide is remained in soil, serious consequence caused by pollution is still difficult to disappear in a short time
It removes.Polycyclic aromatic hydrocarbon (PAHs) is typical persistence organic pollutant common in a kind of soil environment, and concentration level is from μ g/
Kg rises to mg/kg magnitude, and main farm produce PAHs exceeding standard rate reaches 20% or so.Facility agricultural soil is all deposited organic mostly
The harm of combined pollution.
Therefore, carry out the new technology that facility agricultural soil organic composite in outskirts of a town pollutes polynary resistance control and enhancement microbiological reparation
Research reduces population health risk, has great social benefit and environment for improving city and suburbs soil environment quality
Benefit.But lacks the practical technique of cost-effective organic composite polluted farmland soil reparation always so far, especially lack
The weary green recovery technique that still can carry out production activity while repairing agricultural land soil, obtain economic well-being of workers and staff.
Currently, organic polluted soil recovery technique mainly has peripheral doses, chemical remediation and the methods of biological prosthetic.Physics
It repairs and mainly such as strips using the method for physics, fills removal or fixed-contamination object.But the project amount that the technology is implemented is larger,
Implementation cost is higher, limits its popularization and application to a certain extent.Chemical remediation generally using the soil body apply enhancing agents or
Organic pollutant is eluted or is fixed in soil by the method for fixating reagent, and then improves the mobility or stagnant of organic pollutant
The property stayed.But the nutrients such as Ca necessary to plant and Mg are also eluted rhizosphere while washed off soil pollutant by leacheate,
Cause the missing of plant nutrient.Biological prosthetic be fixed with using microorganism (such as bacterium, fungi) degradation or plant absorption
Machine pollutant, it is biological prosthetic easy to operate, at low cost and be not likely to produce secondary pollution compared with physical chemistry reparation, thus by
Favor.During organic polluted soil is biological prosthetic, plant and microorganism are typically only capable to absorb and degrade molten in the soil liquid
The organic pollutant of state is solved, remediation efficiency is lower.Surfactant can promote organic pollutant to discharge from soil, make its point
It is fitted in surfactant micellar or is adsorbed on surfactant monomer, improve microbial degradation rate and biological effectiveness.Cause
This, repairs compound organic polluted soil using surfactant-plant-microorganism joint, remediation efficiency not only can be improved, and
And the chemical substance of microorganism secretion can reduce the toxic action that plant is subject to again, promote plant growth, meanwhile, select low product
Tired type plant, aerial part absorbs and enrichment DDTs and PAHs content is well below conventional plant, in safe edible standard
When, it still can be used as edible vegetable and obtain economic benefit.
Summary of the invention
It is an object of the invention to provide a kind of method that joint repairs DDTs-PAHs combined contamination soil, i.e. plant-is micro-
Biology-surfactant joint repairs the agrological method of DDTs-PAHs combined pollution.This method has to environmental disruption
It is small, do not generate secondary pollution, it is low-cost, be easy to operate in large scale, do not influence production and be a kind of peace the advantages of income
Based technique for in-situ remediation that is entirely effective, can producing when repairing, is suitble to the facility agricultural soil reparation of middle low pollution.
The technical solution adopted by the present invention are as follows:
Rhamnolipid fortification of plants-microbial association repairs the agrological method of DDTs-PAHs combined pollution, this method
Be spray rhanolipid as biosurfactant in organo-chlorine pesticide DDT and polycyclic aromatic hydrocarbon composite pollution agricultural soil, and
It is inoculated with indigenous mixed degradation bacterium (Methylotrophic bacillus and Arthrobacter globiformis), while planting low accumulation type vegetable crop,
By plant-microorganism-biosurfactant synergistic effect degradation target contaminant, soil in protected field is both removed to realize
In remaining compound organic pollutant, and do not influence production and economic well-being of workers and staff purpose；Original inhabitants' efficient degrading bacteria is DDTs-
The indigenous microorganism Methylotrophic bacillus filtered out in PAHs combined pollution agricultural soil and Arthrobacter globiformis；The table
Face activating agent is rhamnolipid；The plant is low accumulation type vegetable crop.
Mixed degradation bacterium is Methylotrophic bacillus and Arthrobacter globiformis, Methylotrophic bacillus
(Bacillus methylotrophicus), it is general that on May 18th, 2016 is preserved in China Committee for Culture Collection of Microorganisms
Logical microorganism center, deposit number: CGMCC No.12461, preservation address: Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3.Ball
Shape arthrobacterium (Arthrobacter globiformis) is preserved in Chinese microorganism strain preservation management committee on March 16th, 2016
Member's meeting common micro-organisms center, deposit number: CGMCC No.12220, preservation address: Yard 1, BeiChen xi Road, Chaoyang District, Beijing City
No. 3.
The specific implementation step of the method for the present invention is as follows:
1. facility agricultural soil dries 1 under natural conditions by tentatively turning over (ploughed depth be 20~40 cm), smooth
~2 weeks, then by laboratory screening go out efficient degrading bacteria Methylotrophic bacillus and Arthrobacter globiformis Mixed Microbes are made
Suspension is uniformly sprayed on contaminated soil, and the inoculum amount of soil is 100~300 L/ha, and inoculum density is 0.5~1.5
×109Cfu/g soil, at once mixes Soil tillage in 12 h after sprinkling；
2. the application concentration of rhamnolipid is 5~20mg/kg, prepared rhamnolipid solution is uniformly sprayed on
On soil surface, soil is carried out secondary mixing at once after adding by surfactant；
Make 3. being filtered out by laboratory potted plant experiment and absorbing the low low accumulation type vegetables of DDTs-PAHs concentration in cauline leaf
Object.Full crop seed is chosen, 6 after the efficient mixed degradation bacterium of soil inoculation and sprinkling surfactant rhamnolipid~
Sowing in 12h.Sowing of vegetable mode is drilling, and application rate is 0.5~0.8 kg/acre, and depth of planting is 2.0~3.0cm, is broadcast
Nitrogen-phosphorus-potassium compound fertilizer is first applied in every band before kind, then earthing, topsoil sow full, free from insect pests vegetable seeds.
4. after planting plants, according to industrialized agriculture Routine Management method, periodically watering, fertilising, weeding, and keeping field
Water-holding capacity 60%~75%.
5. by repeating step 1~step 4, so that it may PAHs and DDTs in pollution facility agricultural soil is removed, until reaching
Its environmental safety standard.
The invention has the advantages that with the utility model has the advantages that
The present invention combines PAHs-DDTs mixed degradation bacterium and low accumulation type vegetables by rhanolipid as biosurfactant
Crop repairs PAHs-DDTs organic composite polluted farmland soil.The rhanolipid as biosurfactant that this method uses has
Low toxicity/non-toxic nature, and can be biodegradable, it not will cause soil secondary pollution.And pass through application Bio-surface active
The DDTs and PAHs that agent can cooperate with solubilising elution to be adsorbed on soil, and then significantly improve the degradation efficiency of microbial degradation bacterium.
Mixed degradation bacterium is inoculated in the present invention can obtain higher repairing effect compared with single degradation bacteria.In addition, passing through experiment
Room filters out low accumulation type vegetable crop, and secretions from plant roots can further promote in soil in joint implant plant process
The decomposition of DDTs and PAHs.Since the concentration of pollutant and enriching quantity are lower in its ground cauline leaf of low accumulation type vegetable crop,
When in food consumption safe range, it can be realized and repaired in production, it will one kind as middle low pollution agricultural soil future
Reparation trend.
Detailed description of the invention
Fig. 1 is (after 70 days) residual quantity and degradation of different disposal group PAHs in soil (16 Priority PAHs)
Rate, from left to right: (1) mixed degradation bacterium+5mg/kg rhamnolipid+rape (RL5)；(2) mixed degradation bacterium+10mg/kg sandlwood
Glycolipid+rape (RL10)；(3) mixed degradation bacterium+20mg/kg rhamnolipid+rape (RL20)；(4) it is not added with any substance
Natural soils (CK).
Fig. 2 be (after 70 days) DDT in different disposal group soil (p, p'-DDT+o, p'-DDT+p, p'-DDE+
P, p'-DDD) residual quantity and degradation rate, from left to right: (1) mixed degradation bacterium+5mg/kg rhamnolipid+rape (RL5)；(2)
Mixed degradation bacterium+10mg/kg rhamnolipid+rape (RL10)；(3) mixed degradation bacterium+20mg/kg rhamnolipid+rape
(RL20)；(4) natural soils (CK) of any substance are not added with.
Specific embodiment
Embodiment 1: facility agricultural soil in-situ immobilization test
This experiment is tested using field repair, and sample plot selects in Shenyang region industrialized agriculture base.First by facility
Soil is turned over (ploughed depth be 20~40 cm), smooth in greenhouse, is dried 1~2 week under natural conditions.Then by soil
It is divided into the native lattice of 1.5 m × 1.5 m square, there are 40-50cm wide interval bands among every two soil lattice.Four kinds of processing are done altogether: (1)
The control treatment added nothing；(2) mixed degradation bacterium+5mg/kg rhamnolipid+rape (RL5)；(3) mixed degradation bacterium+
10mg/kg rhamnolipid+rape (RL10)；(4) mixed degradation bacterium+20mg/kg rhamnolipid+rape (RL20).
Specific implementation step is as follows:
(1) by laboratory screening go out efficient degrading bacteria Methylotrophic bacillus and Arthrobacter globiformis Mixed Microbes are made
Suspension is uniformly sprayed on contaminated soil, and the inoculum amount of soil is 100~300 L/ha, and inoculum density is 0.5~1.5
×109Cfu/g soil；(2) rhamnolipid is dissolved into solution with deionized water, be uniformly sprayed on soil surface, applied dense
Degree is respectively 5,10 and 20mg/kg.Soil tillage is mixed at once in 12 h；(3) rape is sowed with drilling method.Each soil
8-10 plantation ridge is set in lattice, first applies nitrogen-phosphorus-potassium compound fertilizer in every ridge, then earthing, topsoil sow full, nothing
The rape seed damaged by worms, application rate are 0.5~0.8 kg/acre, and depth of planting is 2.0~3.0cm；(4) it after planting rape, presses
According to industrialized agriculture Routine Management method, periodically watering, fertilising, weeding, and keep field capacity 60%~75%.Growth of rape 2-
It is harvested at 3 months, and measures pollutant load in soil and aboveground vegetation part.
Embodiment 2: facility agricultural soil in-situ immobilization simulation test
This experiment is tested using field repair, and certain DDT pollutant is manually added in facility farmland soil, balances one section
It is repaired after time.Sample plot selects in Shenyang region industrialized agriculture base.It will be added in soil in facility plastic greenhouse first
Then doses DDT pollutant is turned over (ploughed depth is 20~40 cm), smooth, sunning balance 1 under natural conditions
~March.Then soil is divided into the native lattice of 1.5 m × 1.5 m square, there are 40-50cm wide interval bands among every two soil lattice.
Four kinds of processing: (1) control treatment added nothing are done altogether；(2) mixed degradation bacterium+5mg/kg rhamnolipid+rape
(RL5)；(3) mixed degradation bacterium+10mg/kg rhamnolipid+rape (RL10)；(4) mixed degradation bacterium+20mg/kg rhamnolipid+
Rape (RL20).
Specific implementation step is as follows:
(1) by laboratory screening go out efficient degrading bacteria Methylotrophic bacillus and Arthrobacter globiformis Mixed Microbes are made
Suspension is uniformly sprayed on contaminated soil, and the inoculum amount of soil is 100~300 L/ha, and inoculum density is 0.5~1.5
×109Cfu/g soil；(2) rhamnolipid is dissolved into solution with deionized water, be uniformly sprayed on soil surface, applied dense
Degree is respectively 5,10 and 20mg/kg.Soil tillage is mixed at once in 12 h；(3) rape is sowed with drilling method.Each soil
8-10 plantation ridge is set in lattice, first applies nitrogen-phosphorus-potassium compound fertilizer in every ridge, then earthing, topsoil sow full, nothing
The rape seed damaged by worms, application rate are 0.5~0.8 kg/acre, and depth of planting is 2.0~3.0cm；(4) it after planting rape, presses
According to industrialized agriculture Routine Management method, periodically watering, fertilising, weeding, and keep field capacity 60%~75%.Growth of rape 2-
It is harvested at 3 months, and measures pollutant load in soil and aboveground vegetation part.
The present embodiment can make that this field is studied and technical staff is more intuitive comprehensively understands the invention, but not with any side
The formula limitation present invention.
Claims (3)
1. a kind of method that joint repairs DDTs-PAHs combined contamination soil, it is characterised in that: this method is dripped in organo-chlorine pesticide
Rhanolipid as biosurfactant is sprayed in DDT and polycyclic aromatic hydrocarbon composite pollution agricultural soil, and is inoculated with indigenous mixed degradation
Bacterium, that is, Methylotrophic bacillus and Arthrobacter globiformis, while low accumulation type vegetable crop is planted, indigenous mixed degradation bacterium
For the Methylotrophic bacillus filtered out in DDTs-PAHs combined pollution agricultural soil and Arthrobacter globiformis；Surface-active
Agent is rhamnolipid；Plant is low accumulation type vegetable crop；Specific implementation step is as follows:
(1) soil is by tentatively turning over, and ploughed depth is 20~40 cm, smooth, is dried 1-2 weeks under natural conditions, then will be real
It tests indigenous mixed degradation bacterium Methylotrophic bacillus that room filters out and plastc ring is made in Arthrobacter globiformis, uniformly
It is sprayed on contaminated soil, the inoculum amount of soil is 100~300 L/ha, and inoculum density is 0.5~1.5 × 109cfu/g
Soil at once mixes Soil tillage in 12 h after sprinkling；
(2) the application concentration of rhamnolipid is 5~20mg/kg, and prepared rhamnolipid solution is uniformly sprayed on soil
On surface, soil is carried out secondary mixing at once after adding by surfactant；
(3) it is filtered out by laboratory potted plant experiment and absorbs the low low accumulation type vegetable crop of DDTs-PAHs concentration in cauline leaf；Choosing
Full crop seed is taken, is broadcast in 6~12h after soil inoculation original inhabitants' mixed degradation bacterium and sprinkling surfactant rhamnolipid
Kind.
2. the method that a kind of joint described in accordance with the claim 1 repairs DDTs-PAHs combined contamination soil, it is characterised in that:
Low accumulation type vegetable crop seeding method is drilling, and application rate is 0.5~0.8 kg/acre, and depth of planting is 2.0~3.0cm,
Nitrogen-phosphorus-potassium compound fertilizer is first applied in every band before sowing, then earthing, topsoil sow full, free from insect pests vegetable seeds.
3. the method that a kind of joint described in accordance with the claim 1 repairs DDTs-PAHs combined contamination soil, it is characterised in that:
After planting low accumulation type vegetable crop, according to industrialized agriculture Routine Management method, periodically watering, fertilising, weeding, and field is kept
Water-holding capacity 60%~75%.
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| NL1000297C2 (en) * | 1995-05-04 | 1996-11-05 | Dhv Milieu En Infrastructuur B | Purificn. of soil, ground and slurry contg. cyclic hydrocarbon(s) |
| CN101229550A (en) * | 2007-01-24 | 2008-07-30 | 中国科学院生态环境研究中心 | Method of increasing gesarex polluted soil phyto remediation efficiency |
| ITMI20121827A1 (en) * | 2012-10-26 | 2014-04-27 | Biodermol S R L | PROCESS FOR DECONTAMINATION OF SOIL POLLUTED BY INSECTICIDES |
| CN102941225A (en) * | 2012-11-26 | 2013-02-27 | 中国科学院南京土壤研究所 | Chemistry-microorganism combination restoration method of polycyclic aromatic hydrocarbons and heavy metal composite contaminated site soil |
| CN104668283A (en) * | 2015-02-09 | 2015-06-03 | 浙江大学 | Method for performing in-situ remediation on polycyclic aromatic hydrocarbon contaminated site through surfactant enhanced microbes |
| CN104923558B (en) * | 2015-05-25 | 2017-03-08 | 浙江大学 | The method that surface active agent solubilization eluting enhancement microbiological repairs OCPs contaminated soil |
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