CN110090859A - A kind of method that plant enhancement microbiological repairs PAHs pollution in agricultural land soil - Google Patents

Abstract

A kind of method that plant enhancement microbiological repairs PAHs pollution in agricultural land soil, it is related to a kind of method of repairing polluted soil, this method plants celery on the basis of adding PAHs efficient degrading bacteria, strengthens degradation of the PAHs efficient degrading bacteria to PAHs in agricultural land soil using celery.Celery can play activation and invigoration effect to PAHs efficient degrading bacteria during the growth process, its root exudates can provide good living environment for microorganism, improve the activity and quantity of microorganism, and then by the absorption, conversion and decomposition etc. in microbial metabolism to PAHs in soil, the reparation of P in soil AHs pollution is realized.This method does not use chemical agent, repair process safety, and repair process does not significantly affect celery quality and yield, the target repaired in production may be implemented, and this method is low in cost, method is simple, easy to operate, can be widely applied.

Description

A kind of method that plant enhancement microbiological repairs PAHs pollution in agricultural land soil

Technical field

The present invention relates to a kind of methods of repairing polluted soil, repair farmland more particularly to a kind of plant enhancement microbiological The method that PAHs pollutes in soil.

Background technique

China is the country that one populous, per capita area of cultivated farmland is less, and cultivated land resource is faced with huge pressure.But It is to rest on surrounding medium for a long time with the development of the social economy, more and more polluter is discharged into environment In, and constantly migrated in each surrounding medium.Soil becomes the storage field of numerous polluters as important surrounding medium Institute, meanwhile, these polluters also have an impact the property of soil, decline soil environment quality constantly, especially with PAHs is the harm of the persistent organic pollutants of representative.

Polycyclic aromatic hydrocarbon (Polycyclic Aromatic Hydrocarbons, abbreviation PAHs) refer to more than two phenyl ring with The connected compound of condensed ring form is a kind of organic substance being widely present in the environment, derives from natural source and artificial source, But it is mainly derived from artificial source, such as imperfect combustion, industrial discharge and vehicle exhaust of fossil fuel etc., water solubility is low, Fat-soluble height can be enriched with by food chain, and can be existed for a long time in organism, be not easy to exclude external.It is same with this When, polycyclic aromatic hydrocarbon is a kind of organic pollutant with strong three-induced effect, with extended residual, half volatile and high poison Property, have to human health and environment and seriously endanger, be classified as priority acccess control by numerous international organizations and country has with what is cut down Machine pollutant can enter human body by the crops of farmland production, cause food-safety problem, generate harm to human body. Therefore, with the common concern by the people of various countries and government, it is badly in need of finding a kind of safe and reliable, is repaired in production and pollute agriculture The method of field soil.

Summary of the invention

The purpose of the present invention is to provide a kind of methods that plant enhancement microbiological repairs PAHs pollution in agricultural land soil, originally Invention adds efficient degrading bacteria Methylotrophic bacillus in PAHs polluted farmland soil (Bacillusmethylotrophicus) (deposit number is CGMCC No.12461), then plant celery (Sheep's-parsley (Libanotisseseloides (Fisch. & C. A. Mey. ex Turcz.) Turcz.)), using celery to efficient The purpose of the PAHs pollution in rehabilitating soil in production is realized in the activated reinforced effect of degradation bacteria.

The purpose of the present invention is what is be achieved through the following technical solutions:

A kind of method that plant enhancement microbiological repairs PAHs pollution in agricultural land soil, the method includes following procedure:

(1) preparation of efficient degradation bacterium solution: by active efficient degrading bacteria Methylotrophic bacillus (Bacillusmethylotrophicus) be inoculated into LB liquid medium, the constant temperature in 30 DEG C, 100 turns of shaking table per minute Culture, when bacterial concentration is 1.0 × 10⁸-10¹⁰CFU·mL^-1When, it is spare to obtain efficient degradation bacterium solution；

(2) cultivation of celery: the good Sheep's-parsley seedling of pre-incubation in the greenhouse, when Sheep's-parsley seedling it is long to about 8-9cm high when it is spare；

(3) repair process:

It is first that farmland progress to be repaired is smooth, choose stone, big soil block is crushed；By prepared efficient degrading bacteria Liquid is sprayed according to the ratio uniform of every square metre of 500mL onto agricultural land soil, to agricultural land soil farming layer depth (0-20cm) into Row is digged, and is uniformly distributed colleges and universities' degradation bacteria in the soil；Then, the Sheep's-parsley seedling cultivated in advance, spacing in the rows are transplanted into It is 4-5cm with line-spacing, periodically waters in Sheep's-parsley growth period, the moisture content of soil is made to keep the 30% of maxmun field capacity, Growth period is 3 months；To improve and guaranteeing remediation efficiency, the soil moisture is kept to exist using the mode for establishing greenhouse or covering with ground sheeting 25-30℃。

The advantages and effects of the present invention are:

The present invention uses Sheep's-parsley to strengthen efficient degrading bacteria Methylotrophic bacillus for the first time and repairs PAHs polluted farmland soil, is A kind of in-situ remediation method for repairing PAHs polluted farmland soil.This method is planted on the basis of adding PAHs efficient degrading bacteria Celery strengthens degradation of the PAHs efficient degrading bacteria to PAHs in agricultural land soil using celery.Celery is efficient during the growth process Degradation bacteria provides good living environment, is conducive to the activity and quantity that improve degradation bacteria, and then pass through efficient degrading bacteria To absorption, conversion and decomposition of PAHs in soil etc. in metabolic process, the reparation of PAHs pollutant in polluted farmland soil is realized. This method does not use chemical agent, repair process safety, and this method is low in cost, method is simple, easy to operate, rehabilitating soil While middle PAHs, celery quality and yield is not significantly affected, realizes what polluted farmland soil was repaired in production Target can be promoted with large area.

The present invention plants Sheep's-parsley in PAHs polluted farmland soil and strengthens in the in-situ immobilization of PAHs efficient degrading bacteria, a side The root exudates secreted in the growth course of face Sheep's-parsley can strengthen the repair of efficient degradation microorganism, and then improve micro- The activity of biology；Enable one side root exudates that can have an impact to the biological effectiveness of PAHs in soil.And then reach reinforcing The purpose that PAHs pollutes in efficient degrading bacteria rehabilitating soil.After three months repair, the pedotheque after repairing, measurement are acquired Wherein PAHs concentration is such as not up to repaired target, then is repeatedly repaired, until the PAHs in agricultural land soil reaches soil environment Safety standard realizes the purpose that polluted farmland soil is repaired in production.

Detailed description of the invention

Fig. 1 present invention is shown using the in-situ remediation method that celery strengthens efficient degrading bacteria reparation PAHs polluted farmland soil It is intended to；

Fig. 2 celery strengthens efficient degrading bacteria and only adds the average healing efficiency of PAHs in efficient degrading bacteria experimental group.

Specific embodiment

The following describes the present invention in detail with reference to examples.

The classification naming of biomaterial: Methylotrophic bacillus methylotrophicus, preservation change The unit full name of biological material specimens: China Committee for Culture Collection of Microorganisms's common micro-organisms center, preservation address: north The institute 3 of the Chaoyang District Jing Shi North Star West Road 1, preservation date: on May 18th, 2016, deposit number: CGMCC NO.12461.

Embodiment 1: laboratory simulation experiment

(1) preparation of efficient degradation bacterium solution: by active efficient degrading bacteria Methylotrophic bacillus (Bacillusmethylotrophicus) be inoculated into LB liquid medium, the constant temperature in 30 DEG C, 100 turns of shaking table per minute Culture, when bacterial concentration is 1.0 × 10⁸-10¹⁰CFU·mL^-1When, it is spare to obtain efficient degradation bacterium solution.

(2) cultivation of celery: the good Sheep's-parsley seedling of pre-incubation in the greenhouse, it is long to about 8-9cm Gao Shibei to Sheep's-parsley seedling With.

(3) repair process:

PAHs contaminated soil in the greenhouse in Shenyang City's new city sub-district suburbs is acquired, after removing stone cleaning and sundries, crosses 2mm sieve Afterwards, containing in flowerpot makes depth of soil 20cm.Measuring PAHs mean concentration in soil is 690.78 μ g/kg.It will be prepared Efficient degradation bacterium solution is sprayed according to the ratio uniform of every square metre of 500mL, and is mixed.It is transplanted into the Sheep's-parsley children cultivated in advance Seedling, spacing in the rows and line-spacing are 4-5cm, are periodically watered, and so that the moisture content of soil is kept the 30% of maxmun field capacity, growth period It is 3 months, keeps room temperature at 25-30 DEG C.Only addition efficient degrading bacteria and the control experiment for only planting celery of setting simultaneously Group.Pedotheque before reparation and after repairing 3 months is acquired, and measures the content of PAHs and the plant weight of celery in soil. As a result see Fig. 2.As can be seen from Figure 2 the average healing efficiency of PAHs is in celery reinforcing efficient degrading bacteria experimental group 58.13%, and the average healing efficiency for only adding PAHs in efficient degrading bacteria experimental group is 49.78%, therefore strengthen can be with for celery Efficient degrading bacteria is significantly improved to the remediation efficiency of PAHs in soil.Only plantation celery and celery strengthen efficient degrading bacteria experimental group In plant weight be respectively 69.46 ± 3.21g and 67.92 ± 5.31g, significant change does not occur.

Claims (1)

1. a kind of method that plant enhancement microbiological repairs PAHs pollution in agricultural land soil, which is characterized in that the method includes Following procedure:

(1) preparation of efficient degradation bacterium solution: by active efficient degrading bacteria Methylotrophic bacillus (Bacillusmethylotrophicus) be inoculated into LB liquid medium, the constant temperature in 30 DEG C, 100 turns of shaking table per minute Culture, when bacterial concentration is 1.0 × 10⁸-10¹⁰CFU·mL^-1When, it is spare to obtain efficient degradation bacterium solution；

(2) cultivation of celery: the good Sheep's-parsley seedling of pre-incubation in the greenhouse, when Sheep's-parsley seedling it is long to about 8-9cm high when it is spare；

(3) repair process:

It is first that farmland progress to be repaired is smooth, choose stone, big soil block is crushed；By prepared efficient degrading bacteria Liquid is sprayed according to the ratio uniform of every square metre of 500mL onto agricultural land soil, to agricultural land soil farming layer depth (0-20cm) into Row is digged, and is uniformly distributed colleges and universities' degradation bacteria in the soil；Then, the Sheep's-parsley seedling cultivated in advance, spacing in the rows are transplanted into It is 4-5cm with line-spacing, periodically waters in Sheep's-parsley growth period, the moisture content of soil is made to keep the 30% of maxmun field capacity, Growth period is 3 months；To improve and guaranteeing remediation efficiency, the soil moisture is kept to exist using the mode for establishing greenhouse or covering with ground sheeting 25-30℃。