CN106001097A - Method for combined remediation of DDTs-PAHs combined contaminated soil - Google Patents
Method for combined remediation of DDTs-PAHs combined contaminated soil Download PDFInfo
- Publication number
- CN106001097A CN106001097A CN201610241718.9A CN201610241718A CN106001097A CN 106001097 A CN106001097 A CN 106001097A CN 201610241718 A CN201610241718 A CN 201610241718A CN 106001097 A CN106001097 A CN 106001097A
- Authority
- CN
- China
- Prior art keywords
- soil
- ddts
- pahs
- remediation
- combined
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/08—Reclamation of contaminated soil chemically
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
- B09C1/105—Reclamation of contaminated soil microbiologically, biologically or by using enzymes using fungi or plants
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Environmental & Geological Engineering (AREA)
- Mycology (AREA)
- Soil Sciences (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Health & Medical Sciences (AREA)
- Botany (AREA)
- Processing Of Solid Wastes (AREA)
Abstract
The invention relates to a method for remediation of contaminated soil, in particular to a method for combined remediation of DDTs-PAHs combined contaminated soil. Biosurfactant rhamnolipid is combined with efficient mixed degrading bacteria to strengthen and promote organic chloride pesticide DDTs and PAHs left in facility agriculture soil for decomposition and conversion; and meanwhile low-accumulation vegetable crops are planted, and microbial growth and degradation of the organic contaminant DDTs and PAHs are further strengthened through the plant root system metabolism and the chemical stimulation function of root exudates. The method has the beneficial effects that damage to the environment is small, no secondary contamination is generated, cost is low, large-scale operation is easy, and the production and economic income are not influenced; the method is a safe and effective in-situ remediation technology capable of achieving remediation and production at the same time; and the method is suitable for remediation of the intermediate and low contamination facility agriculture soil where crops are still planted.
Description
Technical field
The present invention relates to the research field that agricultural soil organic contamination is repaired, the surfactant of organochlorine pesticide clofenotane and polycyclic aromatic hydrocarbon composite pollution-plant-microorganism combined remediation method in a kind of restoration facilities agricultural soil.
Background technology
All the time, people are continuously increased for the sowing amount pursuing higher yield and economic benefit, fertilizer and pesticide.According to statistics, China's annual applications of pesticide amount reaches 50～60 × 107 kg.During pesticide spraying, only the pesticide of 8% can be absorbed by crops, about 40～50% pesticide can fall at upper soll layer.The whole nation is reached 16,000,000 hm by the farmland of pollution by pesticides2, the pesticide exceeding standard rate of main farm produce is up to 16-20%.Although since eighties of last century carries out agriculture chemical registration system the beginning of the eighties, stop producing and using the pesticide such as clofenotane, Niran, heptachlor, but produce owing to China is once a large amount of between 20th century 60～the eighties and uses organochlorine pesticide, soil remains substantial amounts of pesticide, pollutes the serious consequence caused and be the most still difficult to eliminate.Polycyclic aromatic hydrocarbon (PAHs) is typical persistence organic pollutant common in a class soil environment, and its concentration level rises to mg/kg magnitude from g/kg, and main farm produce PAHs exceeding standard rate reaches about 20%.Facility agricultural soil the most all deposits the harm that organic composite pollutes.
Therefore, carry out outskirts of a town facility agricultural soil organic composite and pollute the new industrial research that polynary resistance control is repaired with enhancement microbiological, for improving city and suburbs soil environment quality, reduce population health risk, there is great social benefit and environmental benefit.But lack the practical technique that cost-effective organic composite polluted farmland soil is repaired the most always, particularly lack the green recovery technique still can carried out production activity while repairing agricultural land soil, obtain economic well-being of workers and staff.
At present, organic polluted soil recovery technique mainly has the methods such as peripheral doses, chemical redemption and biological restoration.Peripheral doses is mainly with removal or fixed-contamination things such as the methods of physics such as stripping, landfill.But the quantities of this technology implementation is relatively big, and implementation cost is higher, limits its popularization and application to a certain extent.Chemical redemption typically uses and applies the method for enhancing agents or fixating reagent at the soil body and organic pollution drip washing gone out or be fixed in soil, and then improves mobility or the anelasticity of organic pollution.But the nutrient drip washing such as Ca and Mg required for plant are also gone out rhizosphere while washed off soil pollutant by leacheate, cause the disappearance of plant nutrient.Biological restoration is to utilize microorganism (such as antibacterial, fungus) degraded or plant absorption to be fixed with organic pollutants, and compared with physical chemistry reparation, biological restoration easily operates, low cost and be not likely to produce secondary pollution, thus enjoys favor.During organic polluted soil biological restoration, plant and microorganism are typically only capable to absorb and the organic pollution of the dissolved in the degraded soil liquid, and remediation efficiency is relatively low.Surfactant can promote that organic pollution discharges from soil so that it is is assigned in surfactant micellar or is adsorbed onto on surfactant monomer, improves microbial degradation rate and biological effectiveness.Therefore, surfactant-plant-microorganism combine d bioremediation is used to be combined organic polluted soil, not only can improve remediation efficiency, and, the chemical substance of microorganism secretion can alleviate again the toxic action that plant is subject to, promote plant growing, meanwhile, selecting low accumulation type plant, its aerial parts absorbs and enrichment DDTs and PAHs content is well below conventional plant, time in safe edible standard, still can obtain economic benefit as edible vegetable.
Summary of the invention
A kind of method that it is an object of the invention to provide combine d bioremediation DDTs-PAHs combined contamination soil, i.e. plant-microorganism-surfactant combine d bioremediation agrological method of DDTs-PAHs combined pollution.The method has environmental disruption little, do not produce secondary pollution, low cost, be prone to large-scale operation, do not affect production and the advantage of income, be a kind of safely and effectively, can the based technique for in-situ remediation that produces of reparation limit, limit, oligosaprobic facility agricultural soil reparation in being suitable for.
The technical solution used in the present invention is:
Rhamnolipid fortification of plants-microbial association repairs the agrological method of DDTs-PAHs combined pollution, the method is sprinkling rhanolipid as biosurfactant in organochlorine pesticide clofenotane and polycyclic aromatic hydrocarbon composite pollution agricultural soil, and inoculate original inhabitants mixed degradation bacterium (Methylotrophic bacillus cereus and Arthrobacter globiformis), plant low accumulation type vegetable crop simultaneously, by the synergism degraded target contaminant of plant-microorganism-biosurfactant, thus realize both having removed in soil in protected field residual be compounded with organic pollutants, the most do not affect and produce and the purpose of economic well-being of workers and staff；Described original inhabitants' efficient degrading bacteria is the indigenous microorganism Methylotrophic bacillus cereus and Arthrobacter globiformis filtered out in DDTs-PAHs combined pollution agricultural soil；Described surfactant is rhamnolipid；Described plant is low accumulation type vegetable crop.
Mixed degradation bacterium is Methylotrophic bacillus cereus and Arthrobacter globiformis, Methylotrophic bacillus cereus (Bacillus methylotrophicus), on May 18th, 2016 is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, deposit number: CGMCC No.12461, preservation address: Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3.Arthrobacter globiformis (Arthrobacter globiformis) is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center on March 16th, 2016, deposit number: CGMCC No.12220, preservation address: Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3.
The inventive method to be embodied as step as follows:
1. facility agricultural soil is through tentatively turn over (ploughed depth is 20～40 cm), smooth, dry in the sun 1～2 weeks under natural conditions, then efficient degrading bacteria Methylotrophic bacillus cereus laboratory screening gone out and Arthrobacter globiformis make plastc ring, it is sprayed on uniformly on contaminated soil, the inoculum amount of soil is 100～300 L/ha, and inoculum density is 0.5～1.5 × 109Cfu/g soil, mixes Soil tillage in 12 h after sprinkling at once；
2. the applying concentration of rhamnolipid is 5～20mg/kg, is sprayed on uniformly on soil surface by the rhamnolipid solution prepared, and soil is carried out secondary mixing after adding by surfactant at once；
3. filtered out by laboratory potted plant experiment and stem and leaf absorbs the low accumulation type vegetable crop that DDTs-PAHs concentration is low.Choose full crop seed, sow in soil inoculation high efficient mixed degradation bacteria and in spraying after surfactant rhamnolipid 6～12h.Sowing of vegetable mode is drilling, and application rate is 0.5～0.8 kg/acre, and depth of planting is 2.0～3.0cm, first uses nitrogen-phosphorus-potassium compound fertilizer, then earthing before sowing in every band, and topsoil sows full, the vegetable seeds of free from insect pests.
4. after planting plants, according to industrialized agriculture Routine Management method, periodically water, apply fertilizer, weeding, and keep field capacity 60%～75%.
5. by repeating step 1～step 4, so that it may remove and pollute PAHs and DDTs in facility agricultural soil, until reaching its environmental safety standard.
The invention have the advantages that and beneficial effect:
The present invention repairs PAHs-DDTs organic composite polluted farmland soil by rhanolipid as biosurfactant associating PAHs-DDTs mixed degradation bacterium and low accumulation type vegetable crop.The rhanolipid as biosurfactant that the method uses has low toxicity/non-toxic nature, and can be biodegradable, and does not results in soil secondary pollution.And the solubilising eluting absorption DDTs and PAHs on soil can be worked in coordination with by using biosurfactant, and then significantly improve the degradation efficiency of microbial degradation bacterium.The present invention inoculates mixed degradation bacterium, compared with single degradation bacteria, it is possible to obtain higher repairing effect.It addition, go out low accumulation type vegetable crop by laboratory screening, in joint implant plant process, secretions from plant roots can promote the decomposition of DDTs and PAHs in soil further.Owing to concentration and the enriching quantity of pollutant are relatively low in its ground stem and leaf of low accumulation type vegetable crop, time in food consumption safety range, production limit, limit can be realized and repair, it will become a kind of reparation trend that middle low stain agricultural soil is following.
Accompanying drawing explanation
Fig. 1 is residual quantity and the degradation rate of (after 70 days) different disposal group PAHs in soil (16 Priority PAHs), from left to right: (1) mixed degradation bacterium+5mg/kg rhamnolipid+Brassica campestris L (RL5)；(2) mixed degradation bacterium+10mg/kg rhamnolipid+Brassica campestris L (RL10)；(3) mixed degradation bacterium+20mg/kg rhamnolipid+Brassica campestris L (RL20)；(4) natural soils (CK) of any material it are not added with.
Fig. 2 be clofenotane in (after 70 days) different disposal group soil (p, p'-DDT+o, p'-DDT+
P, p'-DDE+p, p'-DDD) residual quantity and degradation rate, from left to right: (1) mixed degradation bacterium+5mg/kg rhamnolipid+Brassica campestris L (RL5)；(2) mixed degradation bacterium+10mg/kg rhamnolipid+Brassica campestris L (RL10)；(3) mixed degradation bacterium+20mg/kg rhamnolipid+Brassica campestris L (RL20)；(4) natural soils (CK) of any material it are not added with.
Detailed description of the invention
Embodiment 1: facility agricultural soil in-situ immobilization is tested
This experiment uses field repair experiment, and sample plot selects in Shenyang region industrialized agriculture base.First carry out soil in facility plastic greenhouse turning over (ploughed depth is 20～40 cm), smooth, dry in the sun 1～2 weeks under natural conditions.Then soil is divided into 1.5 m × 1.5 m square soil lattice, in the middle of each two soil lattice, leaves 40-50cm wide interval band.Do four kinds of process altogether: (1) whatever without control treatment；(2) mixed degradation bacterium+5mg/kg rhamnolipid+Brassica campestris L (RL5)；(3) mixed degradation bacterium+10mg/kg rhamnolipid+Brassica campestris L (RL10)；(4) mixed degradation bacterium+20mg/kg rhamnolipid+Brassica campestris L (RL20).
It is embodied as step as follows:
(1) efficient degrading bacteria Methylotrophic bacillus cereus laboratory screening gone out and Arthrobacter globiformis make plastc ring, are sprayed on uniformly on contaminated soil, and the inoculum amount of soil is 100～300 L/ha, and inoculum density is 0.5～1.5 × 109Cfu/g soil；(2) rhamnolipid deionized water is dissolved into solution, is sprayed on uniformly on soil surface, apply concentration and be respectively 5,10 and 20mg/kg.At once Soil tillage is mixed in 12 h；(3) Brassica campestris L is sowed with bar broadcast mode.Arranging 8-10 bar plantation ridge in each soil lattice, first use nitrogen-phosphorus-potassium compound fertilizer, then earthing in every ridge, topsoil sows full, the Semen Brassicae campestris of free from insect pests, and application rate is 0.5～0.8 kg/acre, and depth of planting is 2.0～3.0cm；(4), after plantation Brassica campestris L, according to industrialized agriculture Routine Management method, periodically water, apply fertilizer, weeding, and keep field capacity 60%～75%.Gather when growth of rape 2-3 month, and measure pollutant load in soil and aboveground vegetation part.
Embodiment 2: facility agricultural soil in-situ immobilization simulation test
This experiment uses field repair experiment, the certain DDT pollutant of artificial interpolation in facility farmland soil, repairs after balance a period of time.Sample plot selects in Shenyang region industrialized agriculture base.First soil in facility plastic greenhouse will add doses DDT pollutant, then carry out turning over (ploughed depth is 20～40 cm), smooth, dry in the sun balance 1～March under natural conditions.Then soil is divided into 1.5 m × 1.5 m square soil lattice, in the middle of each two soil lattice, leaves 40-50cm wide interval band.Do four kinds of process altogether: (1) whatever without control treatment；(2) mixed degradation bacterium+5mg/kg rhamnolipid+Brassica campestris L (RL5)；(3) mixed degradation bacterium+10mg/kg rhamnolipid+Brassica campestris L (RL10)；(4) mixed degradation bacterium+20mg/kg rhamnolipid+Brassica campestris L (RL20).
It is embodied as step as follows:
(1) efficient degrading bacteria Methylotrophic bacillus cereus laboratory screening gone out and Arthrobacter globiformis make plastc ring, are sprayed on uniformly on contaminated soil, and the inoculum amount of soil is 100～300 L/ha, and inoculum density is 0.5～1.5 × 109Cfu/g soil；(2) rhamnolipid deionized water is dissolved into solution, is sprayed on uniformly on soil surface, apply concentration and be respectively 5,10 and 20mg/kg.At once Soil tillage is mixed in 12 h；(3) Brassica campestris L is sowed with bar broadcast mode.Arranging 8-10 bar plantation ridge in each soil lattice, first use nitrogen-phosphorus-potassium compound fertilizer, then earthing in every ridge, topsoil sows full, the Semen Brassicae campestris of free from insect pests, and application rate is 0.5～0.8 kg/acre, and depth of planting is 2.0～3.0cm；(4), after plantation Brassica campestris L, according to industrialized agriculture Routine Management method, periodically water, apply fertilizer, weeding, and keep field capacity 60%～75%.Gather when growth of rape 2-3 month, and measure pollutant load in soil and aboveground vegetation part.
The present embodiment can make this area research and technical staff comprehensively this invention of understanding more directly perceived, but limits the present invention never in any form.
Claims (3)
1. the method for a combine d bioremediation DDTs-PAHs combined contamination soil, it is characterized in that: the method sprays rhanolipid as biosurfactant in organochlorine pesticide clofenotane and polycyclic aromatic hydrocarbon composite pollution agricultural soil, and inoculate original inhabitants mixed degradation bacterium (Methylotrophic bacillus cereus and Arthrobacter globiformis), planting low accumulation type vegetable crop, original inhabitants' efficient degrading bacteria is the Methylotrophic bacillus cereus and Arthrobacter globiformis filtered out in DDTs-PAHs combined pollution agricultural soil simultaneously；Surfactant is rhamnolipid；Plant is low accumulation type vegetable crop；It is embodied as step as follows:
(1) soil is through tentatively turn over (ploughed depth is 20～40 cm), smooth, dry in the sun 1-2 week under natural conditions, then efficient degrading bacteria Methylotrophic bacillus cereus laboratory screening gone out and Arthrobacter globiformis make plastc ring, it is sprayed on uniformly on contaminated soil, the inoculum amount of soil is 100～300 L/ha, and inoculum density is 0.5～1.5 × 109Cfu/g soil, mixes Soil tillage in 12 h after sprinkling at once；
(2) the applying concentration of rhamnolipid is 5～20mg/kg, is sprayed on uniformly on soil surface by the rhamnolipid solution prepared, and soil is carried out secondary mixing after adding by surfactant at once；
(3) filtered out by laboratory potted plant experiment stem and leaf absorbs the low low accumulation type vegetable crop of DDTs-PAHs concentration, choose full crop seed, sow in 6～12h in soil inoculation high efficient mixed degradation bacteria and after spraying surfactant rhamnolipid.
2. according to the method for a kind of combine d bioremediation DDTs-PAHs combined contamination soil described in claim 1, it is characterized in that: sowing of vegetable mode is drilling, application rate is 0.5～0.8 kg/acre, depth of planting is 2.0～3.0cm, before sowing, every band first uses nitrogen-phosphorus-potassium compound fertilizer, then earthing, topsoil sows full, the vegetable seeds of free from insect pests.
3. according to the method for a kind of combine d bioremediation DDTs-PAHs combined contamination soil described in claim 1, it is characterised in that: after planting plants, according to industrialized agriculture Routine Management method, periodically water, apply fertilizer, weeding, and keep field capacity 60%～75%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610241718.9A CN106001097B (en) | 2016-04-19 | 2016-04-19 | A method of joint repairs DDTs-PAHs combined contamination soil |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610241718.9A CN106001097B (en) | 2016-04-19 | 2016-04-19 | A method of joint repairs DDTs-PAHs combined contamination soil |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106001097A true CN106001097A (en) | 2016-10-12 |
| CN106001097B CN106001097B (en) | 2019-04-23 |
Family
ID=57081526
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610241718.9A Active CN106001097B (en) | 2016-04-19 | 2016-04-19 | A method of joint repairs DDTs-PAHs combined contamination soil |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106001097B (en) |
Cited By (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106734187A (en) * | 2017-01-05 | 2017-05-31 | 沈阳大学 | A kind of method for strengthening combine d bioremediation polycyclic aromatic hydrocarbon pollution |
| CN106825034A (en) * | 2017-01-05 | 2017-06-13 | 沈阳大学 | A kind of method of number of rings PAHs high in efficient degradation soil |
| CN106825033A (en) * | 2017-01-05 | 2017-06-13 | 沈阳大学 | A kind of biological reinforced method for repairing DDT polycyclic aromatic hydrocarbon composite pollution soil |
| CN106862262A (en) * | 2017-03-24 | 2017-06-20 | 四川大学 | A kind of soil biochemistry complex repairation agent and preparation method and application |
| CN107903910A (en) * | 2017-11-16 | 2018-04-13 | 中国环境科学研究院 | It is a kind of for the biological agent of contaminated soil remediation and its preparation, application method |
| CN108687131A (en) * | 2017-12-07 | 2018-10-23 | 苏州市环科环保技术发展有限公司 | Chemical pollution site remediation method |
| CN109226255A (en) * | 2018-11-28 | 2019-01-18 | 杭州滨江区市政园林工程有限公司 | A kind of restorative procedure of organic polluted soil |
| CN109504641A (en) * | 2018-12-31 | 2019-03-22 | 嘉兴益方环境科技有限公司 | Bacillus subtilis DDT98806 and its application |
| CN110090859A (en) * | 2019-04-12 | 2019-08-06 | 沈阳大学 | A kind of method that plant enhancement microbiological repairs PAHs pollution in agricultural land soil |
| CN110180888A (en) * | 2019-04-15 | 2019-08-30 | 沈阳大学 | A kind of method that blood meal enhancement microbiological repairs DDTs polluted farmland soil |
| CN110420996A (en) * | 2019-08-21 | 2019-11-08 | 江西青赣环境科技有限公司 | A kind of restoration of the ecosystem heavy-metal contaminated soil administering method |
| CN111420984A (en) * | 2020-05-06 | 2020-07-17 | 辽宁大学 | Method for repairing petroleum-polluted soil by combining plant-bacterium-fungus-rhamnolipid |
| CN112570447A (en) * | 2020-11-23 | 2021-03-30 | 陕西欧菲德环保科技有限公司 | Composite remediation method for petroleum hydrocarbon contaminated soil |
| CN112893441A (en) * | 2021-01-19 | 2021-06-04 | 沈阳大学 | Method for combined enhanced phytoremediation of polycyclic aromatic hydrocarbon-polluted farmland soil |
| CN113234618A (en) * | 2021-04-08 | 2021-08-10 | 西安石油大学 | Composite microbial agent for treating oily sludge and use method thereof |
| CN115041519A (en) * | 2022-06-14 | 2022-09-13 | 沈阳大学 | Method for efficiently degrading soil PAHs and DDTs composite pollution by combining enhanced microorganism sequential anaerobic-aerobic |
| CN115247066A (en) * | 2022-07-12 | 2022-10-28 | 生态环境部南京环境科学研究所 | Remediation agent for pesticide chemical polluted site and preparation method thereof |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| NL1000297C2 (en) * | 1995-05-04 | 1996-11-05 | Dhv Milieu En Infrastructuur B | Purificn. of soil, ground and slurry contg. cyclic hydrocarbon(s) |
| CN101229550A (en) * | 2007-01-24 | 2008-07-30 | 中国科学院生态环境研究中心 | Method of increasing gesarex polluted soil phyto remediation efficiency |
| CN102941225A (en) * | 2012-11-26 | 2013-02-27 | 中国科学院南京土壤研究所 | Chemistry-microorganism combination restoration method of polycyclic aromatic hydrocarbons and heavy metal composite contaminated site soil |
| EP2727662A1 (en) * | 2012-10-26 | 2014-05-07 | Biodermol Ambiente S.r.l. | Process for reducing levels of contamination of soils polluted by DDT, DDE, DDD and/or isomers thereof |
| CN104668283A (en) * | 2015-02-09 | 2015-06-03 | 浙江大学 | Method for performing in-situ remediation on polycyclic aromatic hydrocarbon contaminated site through surfactant enhanced microbes |
| CN104923558A (en) * | 2015-05-25 | 2015-09-23 | 浙江大学 | Method for remedying soil polluted by organo-chlorine pesticides (OCPs) on basis of solubilization, elution and microorganism strengthening effects of surface active agent |
-
2016
- 2016-04-19 CN CN201610241718.9A patent/CN106001097B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| NL1000297C2 (en) * | 1995-05-04 | 1996-11-05 | Dhv Milieu En Infrastructuur B | Purificn. of soil, ground and slurry contg. cyclic hydrocarbon(s) |
| CN101229550A (en) * | 2007-01-24 | 2008-07-30 | 中国科学院生态环境研究中心 | Method of increasing gesarex polluted soil phyto remediation efficiency |
| EP2727662A1 (en) * | 2012-10-26 | 2014-05-07 | Biodermol Ambiente S.r.l. | Process for reducing levels of contamination of soils polluted by DDT, DDE, DDD and/or isomers thereof |
| CN102941225A (en) * | 2012-11-26 | 2013-02-27 | 中国科学院南京土壤研究所 | Chemistry-microorganism combination restoration method of polycyclic aromatic hydrocarbons and heavy metal composite contaminated site soil |
| CN104668283A (en) * | 2015-02-09 | 2015-06-03 | 浙江大学 | Method for performing in-situ remediation on polycyclic aromatic hydrocarbon contaminated site through surfactant enhanced microbes |
| CN104923558A (en) * | 2015-05-25 | 2015-09-23 | 浙江大学 | Method for remedying soil polluted by organo-chlorine pesticides (OCPs) on basis of solubilization, elution and microorganism strengthening effects of surface active agent |
Non-Patent Citations (2)
| Title |
|---|
| 杨柳燕等: "POPs的微生物降解进展", 《持久性有机污染物论坛2006暨第一届持久性有机污染物全国学术研讨会论文集》 * |
| 毛华军等: "多环芳烃污染土壤表面活性剂清洗及生物柴油强化", 《农业环境科学学报》 * |
Cited By (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106734187A (en) * | 2017-01-05 | 2017-05-31 | 沈阳大学 | A kind of method for strengthening combine d bioremediation polycyclic aromatic hydrocarbon pollution |
| CN106825034A (en) * | 2017-01-05 | 2017-06-13 | 沈阳大学 | A kind of method of number of rings PAHs high in efficient degradation soil |
| CN106825033A (en) * | 2017-01-05 | 2017-06-13 | 沈阳大学 | A kind of biological reinforced method for repairing DDT polycyclic aromatic hydrocarbon composite pollution soil |
| CN106862262A (en) * | 2017-03-24 | 2017-06-20 | 四川大学 | A kind of soil biochemistry complex repairation agent and preparation method and application |
| CN106862262B (en) * | 2017-03-24 | 2020-08-04 | 四川大学 | Soil biochemical composite repairing agent and preparation method and application thereof |
| CN107903910A (en) * | 2017-11-16 | 2018-04-13 | 中国环境科学研究院 | It is a kind of for the biological agent of contaminated soil remediation and its preparation, application method |
| CN107903910B (en) * | 2017-11-16 | 2019-12-03 | 中国环境科学研究院 | For the biological agent of organic polluted soil reparation and its preparation, application method |
| CN108687131A (en) * | 2017-12-07 | 2018-10-23 | 苏州市环科环保技术发展有限公司 | Chemical pollution site remediation method |
| CN109226255A (en) * | 2018-11-28 | 2019-01-18 | 杭州滨江区市政园林工程有限公司 | A kind of restorative procedure of organic polluted soil |
| CN109504641A (en) * | 2018-12-31 | 2019-03-22 | 嘉兴益方环境科技有限公司 | Bacillus subtilis DDT98806 and its application |
| CN109504641B (en) * | 2018-12-31 | 2022-04-01 | 嘉兴益方环境科技有限公司 | Bacillus subtilis DDT98806 and application thereof |
| CN110090859A (en) * | 2019-04-12 | 2019-08-06 | 沈阳大学 | A kind of method that plant enhancement microbiological repairs PAHs pollution in agricultural land soil |
| CN110180888A (en) * | 2019-04-15 | 2019-08-30 | 沈阳大学 | A kind of method that blood meal enhancement microbiological repairs DDTs polluted farmland soil |
| CN110420996A (en) * | 2019-08-21 | 2019-11-08 | 江西青赣环境科技有限公司 | A kind of restoration of the ecosystem heavy-metal contaminated soil administering method |
| CN111420984A (en) * | 2020-05-06 | 2020-07-17 | 辽宁大学 | Method for repairing petroleum-polluted soil by combining plant-bacterium-fungus-rhamnolipid |
| CN112570447A (en) * | 2020-11-23 | 2021-03-30 | 陕西欧菲德环保科技有限公司 | Composite remediation method for petroleum hydrocarbon contaminated soil |
| CN112893441A (en) * | 2021-01-19 | 2021-06-04 | 沈阳大学 | Method for combined enhanced phytoremediation of polycyclic aromatic hydrocarbon-polluted farmland soil |
| CN113234618A (en) * | 2021-04-08 | 2021-08-10 | 西安石油大学 | Composite microbial agent for treating oily sludge and use method thereof |
| CN115041519A (en) * | 2022-06-14 | 2022-09-13 | 沈阳大学 | Method for efficiently degrading soil PAHs and DDTs composite pollution by combining enhanced microorganism sequential anaerobic-aerobic |
| CN115041519B (en) * | 2022-06-14 | 2023-09-08 | 沈阳大学 | Method for efficiently degrading soil PAHs and DDTs combined pollution by combining enhanced microorganism sequence anaerobic-aerobic |
| CN115247066A (en) * | 2022-07-12 | 2022-10-28 | 生态环境部南京环境科学研究所 | Remediation agent for pesticide chemical polluted site and preparation method thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN106001097B (en) | 2019-04-23 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106001097B (en) | A method of joint repairs DDTs-PAHs combined contamination soil | |
| US9333382B2 (en) | Enhanced combination method for phytoremediation of petroleum contaminated soil by Impatiens balsamina L. associated with the indigenous microorganisms | |
| CN105149345B (en) | A kind of microorganism, plant, the method for charcoal collaboration repairing heavy metal in soil pollution | |
| CN106613588A (en) | Method for cyclically repairing cadmium-contaminated rice field and planting rice | |
| CN105945047A (en) | Comprehensive remediation method for soil polluted by heavy metals and application of comprehensive remediation method | |
| CN103586270A (en) | Method for restoring heavy metal contaminated soil | |
| CN101797581A (en) | Ecological method for restoring polycyclic aromatic hydrocarbon polluted farmland soil | |
| CN112090943B (en) | Multi-type land soil ecological restoration system and working method thereof | |
| CN106508160A (en) | Soil improvement method | |
| CN105127189A (en) | Compound method for restoring soil polluted by heavy metal | |
| CN106583431A (en) | Bioremediation method for soil polluted by heavy metal lead cadmium and base on kosteletzkya virginica | |
| CN108655162A (en) | A kind of method of restoring soil with serious petroleum pollution | |
| Herviyanti et al. | Effect of biochar from young coconut waste to improve chemical properties of ultisols and growth coffee [Coffea arabica L.] plant seeds | |
| CN105149332B (en) | A kind of microorganism and the method for plant combined restoration of soil polluted by heavy metal | |
| CN109127693B (en) | Method for restoring Cu, Pb and As polluted soil by inoculating sunflower and castor with bacillus subtilis | |
| US11278944B2 (en) | Nanobioremediation method for soil in high-concentration polycyclic aromatic hydrocarbon (PAH)-contaminated site | |
| CN107552550A (en) | A kind of method that cadmium, lead-contaminated soil are repaired using root exudates fortification of plants | |
| CN108097711A (en) | A kind of method for repairing Grown In Zinc Contaminated Soil using penicillium janthinellum joint Indian mustard | |
| CN110090859A (en) | A kind of method that plant enhancement microbiological repairs PAHs pollution in agricultural land soil | |
| CN109226254A (en) | A kind of method of microorganism and plant combined repairing polluted soil | |
| CN103250487B (en) | Method for processing ginseng and American ginseng seeds by combination of supersonic wave, potassium permanganate and microbial inoculum | |
| CN102151692B (en) | Method for repairing nitrobenzene compound contaminated soil by utilizing flax | |
| CN104560836A (en) | Bacillus licheniformis and application thereof in degradation of beta-cypermethrin | |
| CN107155440A (en) | A kind of method of the fast quick-recovery agricultural continuous cropping soil soil fertility of utilization complex microorganism | |
| CN113263048A (en) | Layered treatment method for heavy metal contaminated soil |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20201222 Address after: People's Government of Jinshan Town, building 15, Qingshan langu community, Tangquan Road, Jinshan Town, Yuanbao District, Dandong City, Liaoning Province Patentee after: Dandong Jinshan Investment Construction Development Co.,Ltd. Address before: 110044 Liaoning Province, Shenyang City District wanghuanan Street No. 21 Patentee before: SHENYANG University |