CN106734187A - A kind of method for strengthening combine d bioremediation polycyclic aromatic hydrocarbon pollution - Google Patents
A kind of method for strengthening combine d bioremediation polycyclic aromatic hydrocarbon pollution Download PDFInfo
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- CN106734187A CN106734187A CN201710006204.XA CN201710006204A CN106734187A CN 106734187 A CN106734187 A CN 106734187A CN 201710006204 A CN201710006204 A CN 201710006204A CN 106734187 A CN106734187 A CN 106734187A
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- soil
- aromatic hydrocarbon
- polycyclic aromatic
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
- B09C1/105—Reclamation of contaminated soil microbiologically, biologically or by using enzymes using fungi or plants
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C2101/00—In situ
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- Environmental & Geological Engineering (AREA)
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Abstract
A kind of method for strengthening combine d bioremediation polycyclic aromatic hydrocarbon pollution, is related to a kind of method of repairing polluted soil, methods described to include procedure below：On-site inspection, levels land, actication of culture, configuration medicament, live in-situ immobilization operation：During ready lignin and rhamnolipid solution applied into soil to be repaired according to regulation concentration, it is sufficiently mixed soil, again to uniform sprinkling 500mL plant growth-promoting bacterias bacterium solution and 500mL efficient degrading bacteria bacterium solutions on every square metre of soil to be repaired, fully mix again, sow rape seed；Final-period management, quality testing, if remain polycyclic aromatic hydrocarbon in soil can not continue to repeat the above steps up to reparation expection, until the polycyclic aromatic hydrocarbon content in soil reaches safety of soil environment standard.The present invention biological reinforced dose of activating soil pollutant of application, and plant enhancement microbiological activity is induced, with large-area applications and environment-friendly, and can will not promote enrichment of the rape to polycyclic aromatic hydrocarbon, application prospect is extensive.
Description
Technical field
The present invention relates to a kind of method of repairing polluted soil, more particularly to a kind of reinforcing combine d bioremediation polycyclic aromatic hydrocarbon is dirty
The method for contaminating soil.
Background technology
At present, the potential risk that pollutant is brought by agricultural product in agricultural land soil has become Environmental security field
The one big focus of attention.With the raising of country and the food-safe problem attention rate of the public, rapid test paper environment is subject to more next
More extensive concern, soil is the core dielectric of environment, and soil pollution has catholicity and seriousness.Therefore, it is badly in need of exploitation warp
The practical technique that Ji, soil Fl efficiently, safe are alleviated and repaired, to ensure that Removed In Soil-crop System ecological environment is pacified
Entirely, the sustainable utilization of Agricultural Clean and land resource is realized.
Polycyclic aromatic hydrocarbon is a kind of persistence organic pollutant, and the imperfect combustion of fossil fuel is the master that polycyclic aromatic hydrocarbon is produced
Approach is wanted, it can be assembled by atmospheric sedimentation effect in soil, and enter into food chain by plants enriched effect, and it enters
Three cause effects are shown as after entering food chain（Teratogenesis, carcinogenic, mutagenesis）Human health is threatened.And with fossil energy
Constantly consumption, if do not carried out effective degradation treatment to polycyclic aromatic hydrocarbon, PAHs in soil certainly will cause tight into cumulative trend
The ecological risk of weight, the necessity and urgency of polycyclic aromatic hydrocarbons contaminated reparation are well imagined.So in the urgent need to developing a kind of skill
Art, makes the polycyclic aromatic hydrocarbon efficient degradation in soil.
There are peripheral doses, chemical remediation, biological prosthetic and connection for persistence organic pollutant contaminated soil remediation technology
Close reparation etc..The rehabilitation cost of peripheral doses and chemical remediation is high and is also easy to produce secondary pollution, and biological prosthetic then remediation efficiency
It is relatively low.
The content of the invention
It is an object of the invention to provide a kind of method for strengthening combine d bioremediation polycyclic aromatic hydrocarbon pollution, the present invention is connection
The one kind in restorative procedure is closed, is to apply biological reinforced dose of activating soil pollutant, and induce plant enhancement microbiological activity
A kind of restorative procedure.The technology with large-area applications and environment-friendly, and can will not promote enrichment of the rape to polycyclic aromatic hydrocarbon, should
It is extensive with prospect.
The purpose of the present invention is achieved through the following technical solutions：
A kind of method for strengthening combine d bioremediation polycyclic aromatic hydrocarbon pollution, methods described includes procedure below：
1）On-site inspection, levels land：0 ~ 20cm of contaminated soil is preset for repair layer, repair layer soil of fully turning over,
Discard the physicochemical properties such as debris in soil, the investigation soil weight；
2）Actication of culture, configures medicament；Efficient degrading bacteria（Bacillus methylotrophicus, it is preserved in China Microbiological
Culture presevation administration committee common micro-organisms center, address is located at Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, during preservation
Between be on May 18th, 2016, deposit number：12461）And promoting growth of plants bacterium promoting growth of plants bacterium（Staphylococcus pasteuri, China Committee for Culture Collection of Microorganisms's common micro-organisms center is preserved in, address is exposed to the sun positioned at Beijing
The institute 3 of area North Star West Road 1, the preservation time is on December 5th, 2016, and deposit number is 13423）Strain early stage is in culture presevation
Storehouse freezen protective, flat board activation must be carried out before by strain, after activation under the conditions of 30 DEG C lucifuge culture 2 days, in aseptic bar
Be seeded in liquid culture medium respectively under part, using shaking table under the conditions of 30 DEG C, 120 rpms constant-temperature shaking culture, periodically
Observation bacterial concentration, after about 3d, bacterial concentration reaches 1.0 × 108~1010 CFU·mL-1When, obtain efficient degrading bacteria bacterium solution
With plant growth-promoting bacteria bacterium solution；Rhamnolipid is standby according to 5mg/kg concentration wiring solution-formings according to actual soil weight, according to reality
Rehabilitating soil weight, lignin prepares according to the concentration of 5g/kg soil；
3）Live in-situ immobilization operation：Ready lignin and rhamnolipid solution are applied into earth backing to be repaired according to regulation concentration
In earth, soil is sufficiently mixed, then to uniform sprinkling 500mL plant growth-promoting bacterias bacterium solution and 500mL on every square metre of soil to be repaired
Efficient degrading bacteria bacterium solution, fully mixes again, sows rape seed；
4）Final-period management, quality testing：Moisture content is maintained at 60% of maximum field capacity or so, periodically looks after vegetable plot, two
Harvesting rape after month, detects rape and PAHs in soil concentration, determines that rape quality inspection can list marketing, soil after qualified
If polycyclic aromatic hydrocarbon is remained in earth can not continued to repeat the above steps up to reparation expection, until the polycyclic aromatic hydrocarbon content in soil reaches
Safety of soil environment standard.
Advantages of the present invention is with effect：
1. a kind of method for strengthening combine d bioremediation polycyclic aromatic hydrocarbon pollution of the present invention, the lignin of the application reserves in nature
Abundant but extremely low currently with rate, lignin be able to can also both be improved as the substrate of microorganism Co metabolism organic pollution
Soil, the treatment of wastes with processes of wastes against one another, its application embodies environment-friendly reparation thinking.
2. the rhamnolipid of present invention application belongs to biosurfactant, and applying rhamnolipid can promote to have in soil
The solubilising of organic pollutants thing, increases its biological effectiveness, makes organic pollution be easier to be utilized and rhamnose by efficient degrading bacteria
Fat no biotoxicity in itself.Rapeseed root can provide attachment environment for efficient degrading bacteria in, and efficient degrading bacteria also may be used
Nutrient, stroke syntaxial system are provided with for plant, plant growth-promoting bacteria can promote rape root to grow, then enhance above-mentioned drop
Solution principle.
3. the inventive method applies lignin as common generation using bacillus to the degradation of PAHs in soil
Thank to material, the solubilization in combination with rhanolipid as biosurfactant to organic contaminants in soil is realized efficiently
Remove the purpose of PAHs in soil, with have it is safe and simple, environment-friendly, can large-area applications, repairing effect it is good, without two
The features such as secondary pollution, and can realize being repaired in production, have a extensive future.
Specific embodiment
With reference to embodiment, the present invention is described in detail.
Embodiment：Test field plot
1. on-site inspection, levels land.Experiment place is in the facilities vegetable greenhouse in the sub- street of Shenyang City, Liaoning Province new city, in advance
0 ~ the 20cm for setting contaminated soil is repair layer, repair layer soil of fully turning over, and discards debris in soil, and the investigation soil weight is
1220kg·m-3, polycyclic aromatic hydrocarbon original concentration is 883.85 μ gkg-1。
2. actication of culture, configures medicament.Efficient degrading bacteria and promoting growth of plants bacterium strain early stage are freezed in culture presevation storehouse
Preserve, strain flat board activation must be carried out before, after 30 DEG C of lucifuge cultures 2 days, be seeded in liquid culture medium respectively, use
Shaking table at 30 DEG C, constant-temperature shaking culture under the conditions of 120r/min, routine observation bacterial concentration, after 2.6d, efficient degrading bacteria bacterium solution
Bacteria concentration with plant growth-promoting bacteria liquid is respectively 2.32 × 109CFU·ml-1With 1.93 × 109CFU·ml-1, efficiently dropped
Solution bacterium bacterium solution and plant growth-promoting bacteria bacterium solution.According to the soil weight, it is 244kg/m to calculate actual repair soil weight2, according to the rules
Concentration, every square metre applies 1.22g rhamnolipids with being dissolved in solution and 1.22kg lignin that 200mL water makes.
3. live in-situ immobilization operation.Ready lignin and rhamnolipid solution are applied according to regulation concentration to be repaired
In earth backing earth, it is sufficiently mixed, it is uniformly distributed in soil, then the uniform sprinkling 500mL on every square metre of soil to be repaired
Plant growth-promoting bacteria bacterium solution and 500mL efficient degrading bacteria bacterium solutions, fully mix again, plant rape.
4. final-period management, quality testing.Moisture content is maintained at 60% of maximum field capacity or so, periodically looks after vegetable plot,
Harvesting rape after two months, it is 53.26%, cauline leaf part and root in rape finally to measure the degradation rate of PAHs in soil
Polycyclic aromatic hydrocarbon content be 69.15 ± 2.6 μ gkg-1（Dry sample）With 125.60 ± 6.3 μ gkg-1（Dry sample）.
Claims (1)
1. it is a kind of strengthen combine d bioremediation polycyclic aromatic hydrocarbon pollution method, it is characterised in that methods described include procedure below：
1）On-site inspection, levels land：0 ~ 20cm of contaminated soil is preset for repair layer, repair layer soil of fully turning over,
Discard the physicochemical properties such as debris in soil, the investigation soil weight；
2）Actication of culture, configures medicament；Efficient degrading bacteria and promoting growth of plants bacterium strain early stage in culture presevation storehouse freezen protective,
Strain must be carried out flat board activation before, after activation under the conditions of 30 DEG C lucifuge culture 2 days, be aseptically inoculated with respectively
Into liquid culture medium, using shaking table under the conditions of 30 DEG C, 120 rpms constant-temperature shaking culture, routine observation bacterial concentration,
After about 3d, bacterial concentration reaches 1.0 × 108~1010 CFU·mL-1When, obtain efficient degrading bacteria bacterium solution and plant growth-promoting bacteria bacterium
Liquid；Rhamnolipid is standby according to 5mg/kg concentration wiring solution-formings according to actual soil weight, according to actual repair soil weight,
Lignin prepares according to the concentration of 5g/kg soil；
3）Live in-situ immobilization operation：Ready lignin and rhamnolipid solution are applied into earth backing to be repaired according to regulation concentration
In earth, soil is sufficiently mixed, then to uniform sprinkling 500mL plant growth-promoting bacterias bacterium solution and 500mL on every square metre of soil to be repaired
Efficient degrading bacteria bacterium solution, fully mixes again, sows rape seed；
4）Final-period management, quality testing：Moisture content is maintained at 60% of maximum field capacity or so, periodically looks after vegetable plot, two
Harvesting rape after month, detects rape and PAHs in soil concentration, determines that rape quality inspection can list marketing, soil after qualified
If polycyclic aromatic hydrocarbon is remained in earth can not continued to repeat the above steps up to reparation expection, until the polycyclic aromatic hydrocarbon content in soil reaches
Safety of soil environment standard.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110293127A (en) * | 2019-06-28 | 2019-10-01 | 陕西欧菲德环保科技有限公司 | A kind of closed loop biological renovation method for oil contaminated soil |
| CN112090954A (en) * | 2020-08-31 | 2020-12-18 | 山西大学 | Activated and degraded coagulated beads, preparation method thereof and degradation method of polycyclic aromatic hydrocarbon-polluted soil |
| CN112570447A (en) * | 2020-11-23 | 2021-03-30 | 陕西欧菲德环保科技有限公司 | Composite remediation method for petroleum hydrocarbon contaminated soil |
| CN112893441A (en) * | 2021-01-19 | 2021-06-04 | 沈阳大学 | Method for combined enhanced phytoremediation of polycyclic aromatic hydrocarbon-polluted farmland soil |
| CN113234618A (en) * | 2021-04-08 | 2021-08-10 | 西安石油大学 | Composite microbial agent for treating oily sludge and use method thereof |
| CN114888071A (en) * | 2022-04-25 | 2022-08-12 | 西北工业大学深圳研究院 | Method for in-situ remediation of organic contaminated soil and application |
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| CN112893441A (en) * | 2021-01-19 | 2021-06-04 | 沈阳大学 | Method for combined enhanced phytoremediation of polycyclic aromatic hydrocarbon-polluted farmland soil |
| CN113234618A (en) * | 2021-04-08 | 2021-08-10 | 西安石油大学 | Composite microbial agent for treating oily sludge and use method thereof |
| CN114888071A (en) * | 2022-04-25 | 2022-08-12 | 西北工业大学深圳研究院 | Method for in-situ remediation of organic contaminated soil and application |
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Inventor after: Sun Lina Inventor after: Zheng Xuehao Inventor after: Wang Xiaoxu Inventor after: Liu Kebin Inventor after: Zhang Chao Inventor after: Wang Hui Inventor after: Zhang Hongling Inventor after: Chen Su Inventor before: Sun Lina Inventor before: Wang Xiaoxu Inventor before: Liu Kebin Inventor before: Zhang Chao Inventor before: Wang Hui Inventor before: Zhang Hongling Inventor before: Chen Su |
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Application publication date: 20170531 |