CN105130628A - Efficient submerged fermentation Agrocybe aegerita medium and preparation method therefor - Google Patents

Abstract

The invention discloses an efficient submerged fermentation Agrocybe aegerita medium. The medium comprises the following components in parts by weight: talcum powder, broadleaf tree chip, litchi seed, retinervus citri reticulatae, river snail powder, shrimp powder, tomato vine, small bamboo branches, copper glycinate, zinc glycinate, yeast powder, peptone, limestone, light calcium carbonate, fermental animal dropping, fine sand, marigold leaf, corncob granule, high-protein rice bran, laetiporus sulphureus, alginate, sulfate magnesium, oyster shell powder, silk and an appropriate amount of water. The submerged fermentation Agrocybe aegerita efficient medium adopts the broadleaf tree chip, litchi seed, retinervus citri reticulatae, tomato vine, small bamboo branch, marigold leaf and corncob granule through acid treatment and alkali treatment, the Agrocybe aegerita medium is prepared through submerged fermentation of oyster shell loaded saccharomycetes, so that bacteria and diseases are inhibited, and rich nutrients are provided for the Agrocybe aegerita; and efficient cultivation of Agrocybe aegerita can be realized. According to the medium, the raw material cost is low, the preparation method is simple, the cultivation effect is high, and the medium is worth for generalization.

Description

A kind of submerged fermentation tea tree mushroom efficient culture medium and preparation method thereof

Technical field

The present invention relates to culture medium of edible fungus field, particularly relate to a kind of submerged fermentation tea tree mushroom efficient culture medium and preparation method thereof.

Background technology

Tea tree mushroom is a kind of wood-decay fungi, has effect of heat-clearing, flat liver, improving eyesight, diuresis, invigorating the spleen, is a kind ofly to collect high protein, lower fat, nutrition, health care, physiotherapy and green food all over the body.But tea tree mushroom does not have laccase activity because of its inside, so utilize the ability of xylogen more weak, traditional cultivation, the raw material often adopted causes its yield and quality not high.Submerged fermentation, can obtain a large amount of mycelium and meta-bolites in a short time, and its production efficiency is that traditional cultivation method is incomparable, carries out substratum submerged fermentation research, has great importance.

Edible oyster delicious flavour, nutritious, have the good reputation of " ocean milk ", the edible portion of oyster is just make use of in consumption, but the oyster shells occupying oyster 90% is often abandoned by as rubbish, causes the wasting of resources and environmental pollution, has had a strong impact on the development of oyster culture industry.17 seed amino acid and 24 kinds of trace elements such as iron, zinc, selenium such as oyster shells rich in proteins, glycoprotein and polysaccharide and glycine, Gelucystine, methionine(Met), that organic-biological macromole is as framework, the continuous orderliness of calcium carbonate molecule is piled up, form the tridimensional network with higher density gradually, mainly contain three layers of composition, outermost layer is thickness and thin hardened proteins stratum corneum, middle level is calcareous fiber interweaving, prismatic layer containing more natural pore, mainly contain calcite to form, internal layer is nacreous layer, is mainly made up of aragonite.In the three-decker of oyster, most importantly prismatic layer, becomes bladed structure, containing a large amount of micropore, after calcining, CO2 gets rid of, and material decomposes, duct originally changes, and forms complicated vesicular structure, thus has certain absorption, exchange, catalytic capability.Adsorbed as natural porous by oyster shells at present, use and slow down fertilizer field, play fertilizer conservation, the report improving the effect of utilization rate of fertilizer is a lot, but seldom has the report of the submerged fermentation regulation and control of oyster shells being used substratum.

Silkworm silk is a kind of natural protein fibre, mainly contain the fibroin composition of outer field silk gum and internal layer, good biocompatibility, can promote the proliferation and differentiation of cell, the regeneration of induced tissue cell, without antigenic action, there is biological degradability simultaneously, containing periodic hydrophobe region in structure, make between itself and protein, to there is very strong interaction force, can protein active be kept, have not yet to see the report that silkworm silk regulates and controls for substratum submerged fermentation.

Summary of the invention

The present invention is directed to the Efficient Cultivation demand of tea tree mushroom, a kind of submerged fermentation tea tree mushroom efficient culture medium and preparation method thereof is provided.

The present invention is achieved by the following technical solutions:

A kind of submerged fermentation tea tree mushroom efficient culture medium, comprise following parts by weight of component: talcum powder 2.4-2.7, hardwood sawdust 20-25, Semen Litchi 21-22, tangerine pith 15-17, river snail powder 15-16, sea shrimp med 11-12, tomato seedling 8-9, little bamboo branch 7-8, glycocoll-copper 0.4-0.6, zinc glycinate 0.7-0.9, yeast powder 2.4-2.8, peptone 1.4-1.8, lime 0.5-0.6, lightweight carbonic acid 0.2-0.3, animal fermented ight soil 20-23, fine sand 7-9, Leaf of Aztec Marigold 6-7, corn cob granule 20-30, high protein rice bran 25-29, Laetiporus sulphureus (Bull. Ex Fr.) Bond. Et Singer. 0.8-0.9, Lalgine 1-2, magnesium sulfate 0.9-1.1, oyster shell powder 14-18, silk 2.3-2.5, appropriate water.

A kind of submerged fermentation tea tree mushroom efficient culture medium preparation method, comprises the following steps:

(1) by after oyster shells, talcum powder mixing and stirring, high temperature 180-190 DEG C of calcining 2-3h, obtains compound porous oyster shell powder for subsequent use; By compound porous for half oyster shell powder, half yeast powder, silk, peptone, Laetiporus sulphureus (Bull. Ex Fr.) Bond. Et Singer. and gross weight 1-2 water mixing and stirring doubly, the yeast powder obtaining the loading of compound porous oyster shell powder silk is for subsequent use; By half porous oyster shell powder, glycocoll-copper, zinc glycinate mixing, and add gross weight 2-3 water doubly, moderate-speed mixer 1-1.5h, to evenly, obtains compound porous oyster shell powder loading organic nutrient solution for cultivating for subsequent use;

(2) by lightweight carbonic acid, Lalgine, magnesium sulfate mixing, and add gross weight 2-3 water mixing and stirring doubly, obtain acidotrophy liquid for subsequent use; Stir alkali solution is for subsequent use by adding gross weight 3-4 water doubly in lime; By hardwood sawdust, Semen Litchi, tangerine pith, tomato seedling, little bamboo branch, Leaf of Aztec Marigold, corn cob granule mixing, put into pulverizer and pulverize 20-30min, put into decompressor afterwards to expand 30-40min, obtain expanding powder flour, acidotrophy liquid is evenly admixed by expanding powder flour, leave standstill 2-3d, evenly admix alkali solution more afterwards, obtain modified expanded comminuted powder for subsequent use;

(3) modified expanded comminuted powder, river snail powder, extra large shrimp med, animal fermented ight soil, fine sand, high protein rice bran, compound porous oyster shell powder are loaded organic nutrient solution for cultivating, the activated yeast of residue half and the water mixing of gross weight 3-4 times, after stirring, aerobic fermentation 9-11d at 20.5-23 DEG C, the yeast powder that the compound porous oyster shell powder silk pumping into activation afterwards loads, after stirring, at 17.5-20.5 DEG C, aerobic fermentation 25-29d, obtains fermention medium for subsequent use;

(4) fermention medium is dewatered to moisture content by traditional vacuum to be the 60-66% of gross weight, to adopt polyethylene plastic bag pack afterwards, and in the high-temperature steam of 100 DEG C, adopt the ultraviolet assisted sterilization 15-18h of 115W and get final product.

Advantage of the present invention is:

Agrocybe cylindracea culture medium is prepared in the saccharomycetic submerged fermentation that the present invention adopts acid treatment and alkali-treated hardwood sawdust, Semen Litchi, tangerine pith, tomato seedling, little bamboo branch, Leaf of Aztec Marigold, corn cob granule to load through oyster shells, effectively inhibit miscellaneous bacteria and disease, for tea tree mushroom provides abundant nutrition; The Efficient Cultivation of tea tree mushroom can be realized.Raw materials cost of the present invention is low, and preparation method is simple, and cultivation effect is high, is worthy to be popularized.

Embodiment

A kind of submerged fermentation tea tree mushroom efficient culture medium, comprises following parts by weight of component: talcum powder 2.4, hardwood sawdust 20, Semen Litchi 21, tangerine pith 15, river snail powder 15, extra large shrimp med 11, tomato seedling 8, little bamboo branch 7, glycocoll-copper 0.4, zinc glycinate 0.7, yeast powder 2.4, peptone 1.4, lime 0.5, lightweight carbonic acid 0.2, animal fermented ight soil 20, fine sand 7, Leaf of Aztec Marigold 6, corn cob granule 20, high protein rice bran 25, Laetiporus sulphureus (Bull. Ex Fr.) Bond. Et Singer. 0.8, Lalgine 1, magnesium sulfate 0.9, oyster shell powder 14, silk 2.3, appropriate water.

A kind of submerged fermentation tea tree mushroom efficient culture medium preparation method, comprises the following steps:

(1) by after oyster shells, talcum powder mixing and stirring, high temperature 180 DEG C calcining 2h, obtains compound porous oyster shell powder for subsequent use; By the water mixing and stirring of compound porous for half oyster shell powder, half yeast powder, silk, peptone, Laetiporus sulphureus (Bull. Ex Fr.) Bond. Et Singer. and gross weight 1 times, the yeast powder obtaining the loading of compound porous oyster shell powder silk is for subsequent use; By half porous oyster shell powder, glycocoll-copper, zinc glycinate mixing, and add the water of gross weight 2 times, moderate-speed mixer 1h, to evenly, obtains compound porous oyster shell powder loading organic nutrient solution for cultivating for subsequent use;

(2) by lightweight carbonic acid, Lalgine, magnesium sulfate mixing, and add the water mixing and stirring of gross weight 2 times, obtain acidotrophy liquid for subsequent use; The water adding gross weight 3 times in lime is stirred alkali solution is for subsequent use; By hardwood sawdust, Semen Litchi, tangerine pith, tomato seedling, little bamboo branch, Leaf of Aztec Marigold, corn cob granule mixing, put into pulverizer and pulverize 20min, put into decompressor afterwards to expand 30min, obtain expanding powder flour, acidotrophy liquid is evenly admixed by expanding powder flour, leave standstill 2d, evenly admix alkali solution more afterwards, obtain modified expanded comminuted powder for subsequent use;

(3) modified expanded comminuted powder, river snail powder, extra large shrimp med, animal fermented ight soil, fine sand, high protein rice bran, compound porous oyster shell powder are loaded organic nutrient solution for cultivating, the activated yeast of residue half and the water mixing of gross weight 3 times, after stirring, aerobic fermentation 9d at 20.5 DEG C, the yeast powder that the compound porous oyster shell powder silk pumping into activation afterwards loads, after stirring, at 17.5 DEG C, aerobic fermentation 25d, obtains fermention medium for subsequent use;

(4) fermention medium is dewatered to moisture content by traditional vacuum to be 60% of gross weight, to adopt polyethylene plastic bag pack afterwards, and in the high-temperature steam of 100 DEG C, adopt the ultraviolet assisted sterilization 15h of 115W and get final product.

Claims (2)

1. a submerged fermentation tea tree mushroom efficient culture medium, it is characterized in that, comprise following parts by weight of component: talcum powder 2.4-2.7, hardwood sawdust 20-25, Semen Litchi 21-22, tangerine pith 15-17, river snail powder 15-16, sea shrimp med 11-12, tomato seedling 8-9, little bamboo branch 7-8, glycocoll-copper 0.4-0.6, zinc glycinate 0.7-0.9, yeast powder 2.4-2.8, peptone 1.4-1.8, lime 0.5-0.6, lightweight carbonic acid 0.2-0.3, animal fermented ight soil 20-23, fine sand 7-9, Leaf of Aztec Marigold 6-7, corn cob granule 20-30, high protein rice bran 25-29, Laetiporus sulphureus (Bull. Ex Fr.) Bond. Et Singer. 0.8-0.9, Lalgine 1-2, magnesium sulfate 0.9-1.1, oyster shell powder 14-18, silk 2.3-2.5, appropriate water.

2. a submerged fermentation tea tree mushroom efficient culture medium preparation method, is characterized in that, comprise the following steps:

(1) by after oyster shells, talcum powder mixing and stirring, high temperature 180-190 DEG C of calcining 2-3h, obtains compound porous oyster shell powder for subsequent use; By compound porous for half oyster shell powder, half yeast powder, silk, peptone, Laetiporus sulphureus (Bull. Ex Fr.) Bond. Et Singer. and gross weight 1-2 water mixing and stirring doubly, the yeast powder obtaining the loading of compound porous oyster shell powder silk is for subsequent use; By half porous oyster shell powder, glycocoll-copper, zinc glycinate mixing, and add gross weight 2-3 water doubly, moderate-speed mixer 1-1.5h, to evenly, obtains compound porous oyster shell powder loading organic nutrient solution for cultivating for subsequent use;

(2) by lightweight carbonic acid, Lalgine, magnesium sulfate mixing, and add gross weight 2-3 water mixing and stirring doubly, obtain acidotrophy liquid for subsequent use; Stir alkali solution is for subsequent use by adding gross weight 3-4 water doubly in lime; By hardwood sawdust, Semen Litchi, tangerine pith, tomato seedling, little bamboo branch, Leaf of Aztec Marigold, corn cob granule mixing, put into pulverizer and pulverize 20-30min, put into decompressor afterwards to expand 30-40min, obtain expanding powder flour, acidotrophy liquid is evenly admixed by expanding powder flour, leave standstill 2-3d, evenly admix alkali solution more afterwards, obtain modified expanded comminuted powder for subsequent use;

(3) modified expanded comminuted powder, river snail powder, extra large shrimp med, animal fermented ight soil, fine sand, high protein rice bran, compound porous oyster shell powder are loaded organic nutrient solution for cultivating, the activated yeast of residue half and the water mixing of gross weight 3-4 times, after stirring, aerobic fermentation 9-11d at 20.5-23 DEG C, the yeast powder that the compound porous oyster shell powder silk pumping into activation afterwards loads, after stirring, at 17.5-20.5 DEG C, aerobic fermentation 25-29d, obtains fermention medium for subsequent use;

(4) fermention medium is dewatered to moisture content by traditional vacuum to be the 60-66% of gross weight, to adopt polyethylene plastic bag pack afterwards, and in the high-temperature steam of 100 DEG C, adopt the ultraviolet assisted sterilization 15-18h of 115W and get final product.