CN105106791A - Pharyngitis tea, preparation method thereof and identification method thereof - Google Patents
Pharyngitis tea, preparation method thereof and identification method thereof Download PDFInfo
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- Medicines Containing Plant Substances (AREA)
Abstract
The invention provides pharyngitis tea, a preparation method thereof and an identification method thereof. The pharyngitis tea is prepared from, by weight parts, 5-10 parts of prepared radix stemonae, 5-10 parts of tree peony bark, 5-10 parts of radix ophiopogonis, 5-10 parts of prepared tussilago farfara, 5-10 parts of oroxylum indicum, 10-20 parts of radix isatidis, 5-10 parts of Chinese olive and 10-20 parts of siraitia grosvenorii. The ingredients are smashed, evenly mixed, sieved and subpackaged to obtain the pharyngitis tea. The obtained pharyngitis tea can be made and taken, the total effective rate of treatment can be improved remarkably, qualitative determination is performed on main drugs such as radix ophiopogonis, tree peony bark and the radix stemonae, the interference of the remaining ingredients is eliminated, the blank of a detection method is filled, and the quality standard of the pharyngitis tea is improved.
Description
Technical field
The invention belongs to technical field of Chinese medicine, be specifically related to a kind of pharyngitis tea, its preparation method and authentication method thereof.
Background technology
Pharyngitis is pharyngeal mucosa, the inflammation of submucous tissue, is often a part for upper respiratory tract infection.According to the length of the course of disease and the difference of pathological change character, be divided into acute pharyngitis, the large class of chronic pharyngitis two, wherein, chronic pharyngitis is a kind of disease chronic inflammatory disease slowly, normal to deposit, as sinusitis, proventriculus type remain or retention abscess, pharyngeal bursitis etc. with adjacent organs or systemic disease; Acute pharyngitis is pharyngeal mucous membrane, and under involving mucosa and adenoid acute inflammation, after being often secondary to acute rhinitis or acute tonsil or be the part of upper respiratory tract infection.
The cardinal symptom of acute pharyngolaryngitis is that onset is anxious, from the beginning of time pharyngeal drying, scorching hot, pain then, when swallowing saliva, pharyngalgia is often than more obvious during feed, and can occur together heat, and have a headache, inappetence and extremities aching pain, invade and throat, can neigh and cough by accompanying sound.The cardinal symptom of chronic pharyngolaryngitis is pharyngeal discomfort, does, itches, swollen, the many and causalgia of secretions, easy to dry, has foreign body sensation, not out, can not swallow.Above symptom especially can after slightly many, edible irritable food of speaking, tired or Changes in weather time increase the weight of.
In existing treating pharyngitis method, common drug is oral APC or intramuscular injection antondin etc., or in order to keep oral cavity cleaning, normal employing compound recipe borax solution rinsing the mouth.Inflammation is invaded and throat or trachea, selects suitable antibiotic or hormone atomization inspiration treatment.The person of being in a bad way, selects penicillin intramuscular injection or quiet, and changes the strong antibiotic of effect at any time.Above-mentioned Therapeutic Method not only can not effect a radical cure, and therapeutic process bothers, and easily produces untoward reaction.
Summary of the invention
Must be not enough in prior art in order to solve, the invention provides a kind of pharyngitis tea, its preparation method and authentication method thereof, obtained pharyngitis tea can reconstitute, the total effective rate for the treatment of can be significantly improved, qualitative determination is carried out to principal agent wherein simultaneously, get rid of the interference of all the other components, compensate for the blank of detection method, the perfect quality standard of pharyngitis tea.
To achieve these goals, the present invention is by the following technical solutions:
A kind of pharyngitis tea, be made up of following component by weight: the Radix Stemonae (system) 5-10 part, Cortex Moutan 5-10 part, Radix Ophiopogonis 5-10 part, Flos Farfarae (system) 5-10 part, Semen Oroxyli 5-10 part, Radix Isatidis 10-20 part, Fructus Canarii 5-10 part, Fructus Momordicae 10-20 part.
A kind of preparation method of pharyngitis tea, comprise the following steps: by above-mentioned weight portion, take the Radix Stemonae (system), Cortex Moutan, Radix Ophiopogonis, Flos Farfarae (system), Semen Oroxyli, Radix Isatidis, Fructus Canarii and Fructus Momordicae, pulverize respectively, 80-120 mesh sieve is crossed, after subpackage pack and get final product after mix homogeneously.
In order to Mass Control better, ensure curative effect, to the principal agent in pharyngitis tea, Radix Ophiopogonis, the Radix Stemonae and Cortex Moutan carry out qualitative analysis.
An authentication method for pharyngitis tea, method is as follows:
(1) to the qualification of Radix Ophiopogonis:
(11) preparation of need testing solution: get test sample, add water 40-45min, supersound process, filter with absorbent cotton, filtrate adds hydrochloric acid 2ml, micro-5-6 minute that boils, and filters, filtrate extracts 3 times with chloroform jolting, each 20-25ml, merges chloroform liquid, volatilizes, residue adds chloroform 1ml makes dissolving, as need testing solution;
(12) preparation of reference substance solution: get control medicinal material Radix Ophiopogonis, be made in the same way of reference substance solution;
(13) thin layer chromatography test: draw reference substance solution 5-10 μ l, need testing solution 5-10 μ l, put respectively in same with sodium carboxymethyl cellulose be adhesive silica gel g thin-layer plate on, take volume ratio as the chloroform-acetone of 3-5:1 be developing solvent, launch, take out, dry, spray with volumetric concentration be 10% sulphuric acid-alcoholic solution, at 100-110 DEG C of temperature, be heated to spot development clear; In test sample chromatograph, on the position corresponding to reference substance solution chromatograph, the speckle of aobvious same color;
(2) to the qualification of the Radix Stemonae:
(21) preparation of need testing solution: get test sample, adds ethanol and 1ml hydrochloric acid that 20ml volumetric concentration is 90%, reflux, placement is spent the night, and filters, filtrate evaporate to dryness, the residue 30-35ml that adds water makes dissolving, add more than strong ammonia solution adjust pH to 10, extract 3 times with chloroform jolting, each 25ml, merge chloroform liquid, evaporate to dryness, it is that 90% ethanol makes dissolving, as need testing solution that residue adds 1ml volumetric concentration;
(22) preparation of reference substance solution: get Radix Stemonae control medicinal material, be made in the same way of reference substance solution;
(23) preparation of negative control solution: get medical material except the Radix Stemonae, be made in the same way of negative control solution;
(24) thin layer chromatography test: draw reference substance solution 5-10 μ l, reference substance solution 5-10 μ l, negative control solution 5-10 μ l, put respectively in same with sodium carboxymethyl cellulose be adhesive silica gel g thin-layer plate on, take volume ratio as 8-10:1 chloroform-methanol be developing solvent, lamellae put presaturation 30-40 minute in chromatography cylinder, launch, take out, dry, spray to improve bismuth potassium iodide test solution; In test sample chromatograph, on the position corresponding to reference substance solution chromatograph, aobvious identical orange red speckle;
(3) to the qualification of Cortex Moutan:
(31) preparation of need testing solution: get test sample, add water 50-60ml, chloroform 30-35ml, reflux, let cool, be transferred in separatory funnel, divide and get chloroform liquid, the jolting of water layer chloroform extracts 2 times, each 30ml, merge chloroform liquid, less than 20 DEG C volatilize, residue adds methanol 1ml immediately makes dissolving, as need testing solution;
(32) preparation of reference substance solution: get paeonol reference substance, adds acetone and makes the solution of every 1ml containing 1mg, product solution in contrast;
(33) preparation of negative control solution: get medical material except Cortex Moutan, the method for same step (31) makes negative control;
(34) thin layer chromatography test: draw reference substance solution 5-10 μ l, need testing solution 5-10 μ l, negative control solution 5-10 μ l, put respectively in same with sodium carboxymethyl cellulose be adhesive silica gel g thin-layer plate on, take volume ratio as the cyclohexane-ethyl acetate of 3-4:1 be developing solvent, launch, take out, dry, spray with the acid 5% ferric chloride alcoholic solution of hydrochloric acid, be heated to spot development clear; In test sample chromatograph, on the position corresponding to reference substance solution chromatograph, the speckle of aobvious same color.
Preferably, in above-mentioned steps (11), ultrasonic time is 20-25 minute.
Preferably, in above-mentioned steps (21), reflux process is carried out in a water bath, and the time is 20-25 minute.
Preferably, in above-mentioned steps (31), reflux process is carried out in a water bath, and the time is 30-35 minute.
The pharyngitis tea specification that the present invention obtains is 15g/ bag, and boiled water is taken after being infused in hot water or decoction, once a bag, and twice on the one.
Each components description is as follows:
The Radix Stemonae: the dried root of Stemonaceae plant Radix Stemonae, Radix stemonae japonicae or radix stemonae tuberosae.
Cortex Moutan: the dry root bark of ranunculaceae peony.Clearing away heat and cooling blood, blood circulation promoting and blood stasis dispelling.
Radix Ophiopogonis: the dried root of liliaceous plant Radix Ophiopogonis.YIN nourishing and the production of body fluid promoting, lung moistening clears away heart-fire.For dryness of the lung dry cough, deficiency of YIN consumptive disease is coughed, sore throat pharyngalgia, and Tianjin wound is thirsty, and interior-heat is quenched one's thirst, vexed insomnia, dryness of the intestine constipation.
Flos Farfarae: the dry flower of feverfew coltsfoot.Nourishing the lung to keep the adverse QI downward, relieving cough and resolving phlegm.Cough for new chronic cough, dyspnea and cough with excessive sputum, consumptive disease is coughed hemoptysis.
Semen Oroxyli: the dry mature seed of Bignoniaceae plant oroxylum indicum.Removing heat from the lung and relieving sorethroat, dispersing the stagnated liver-QI for regulating the stomach.For cough due to lung-heat, sore throat, swallows mute, stomachache due to hyperactive liver-QI attacking the stomach.
Radix Isatidis: the dry root of cruciferae isatis.Heat-clearing and toxic substances removing, removing heat from blood sore-throat relieving.
Fructus Canarii: the dry mature fruit of olive subject plant Fructus Canarii albi.Heat-clearing and toxic substances removing, sore-throat relieving, promotes the production of body fluid.For laryngopharynx swelling and pain, cough with ropy sputum, dysphoria with smothery sensation is thirsty, and fish Eriocheir sinensis is poisoning.
Fructus Momordicae: the dry fruit of cucurbitaceous plant Fructus Momordicae.Clearing heat and moistening lung, relieving sore throat to recover voice, loosening bowel to relieve constipation.For lung-heat type cough, dryness of the intestine constipation.
Beneficial effect: the present invention has the following advantages:
(1) therapeutic process is simple, can reconstitute, taking convenience;
(2) do not have side effects, after taking three courses for the treatment of, obvious effective rate is 57.5%, and improvement rate is 37.5%, and total effective rate is 95%;
(3) do not use antibiotic, after patient takes, can not drug resistance be produced;
(4) respectively qualitative determination is carried out to the Radix Ophiopogonis in finished product, the Radix Stemonae and Cortex Moutan, not by the interference of all the other components in pharyngitis tea, compensate for the blank of detection method, the perfect quality standard of pharyngitis tea.
Accompanying drawing explanation
Fig. 1 is the thin layer chromatography qualification comparison diagram of the Radix Ophiopogonis in embodiment 4.
Fig. 2 is the thin layer chromatography qualification comparison diagram of the Radix Stemonae in embodiment 4.
Fig. 3 is the thin layer chromatography qualification comparison diagram of the Cortex Moutan in embodiment 4.
Detailed description of the invention
Embodiment 1
A kind of pharyngitis tea, is made up of following raw material by weight: the Radix Stemonae (system) 5 parts, Cortex Moutan 5 parts, Radix Ophiopogonis 5 parts, Flos Farfarae (system) 5 parts, Semen Oroxyli 5 parts, Radix Isatidis 10 parts, Fructus Canarii 5 parts, Fructus Momordicae 10 parts.
Preparation method: by above-mentioned weight portion, takes the Radix Stemonae (system), Cortex Moutan, Radix Ophiopogonis, Flos Farfarae (system), Semen Oroxyli, Radix Isatidis, Fructus Canarii and Fructus Momordicae, pulverizes respectively, after mix homogeneously cross 80 mesh sieves, subpackage pack after and get final product.
Embodiment 2
A kind of pharyngitis tea, is made up of following raw material by weight: the Radix Stemonae (system) 10 parts, Cortex Moutan 10 parts, Radix Ophiopogonis 10 parts, Flos Farfarae (system) 10 parts, Semen Oroxyli 10 parts, Radix Isatidis 20 parts, Fructus Canarii 10 parts, Fructus Momordicae 20 parts.
Preparation method: by above-mentioned weight portion, takes the Radix Stemonae (system), Cortex Moutan, Radix Ophiopogonis, Flos Farfarae (system), Semen Oroxyli, Radix Isatidis, Fructus Canarii and Fructus Momordicae, pulverizes respectively, after mix homogeneously cross 120 mesh sieves, subpackage pack after and get final product.
Embodiment 3
A kind of pharyngitis tea, be made up of following raw material by weight: the Radix Stemonae (system) 5-10 part, Cortex Moutan 5-10 part, Radix Ophiopogonis 5-10 part, Flos Farfarae (system) 5-10 part, Semen Oroxyli 5-10 part, Radix Isatidis 10-20 part, Fructus Canarii 5-10 part, Fructus Momordicae 10-20 part.
Preparation method: by above-mentioned weight portion, takes the Radix Stemonae (system), Cortex Moutan, Radix Ophiopogonis, Flos Farfarae (system), Semen Oroxyli, Radix Isatidis, Fructus Canarii and Fructus Momordicae, pulverizes respectively, crosses 80-120 mesh sieve after mix homogeneously, after subpackage pack and get final product.
Embodiment 4
An authentication method for pharyngitis tea, method is as follows:
(1) to the qualification of Radix Ophiopogonis:
(11) preparation of need testing solution: get this product 5g, add water 40-45ml, supersound process 20-25 minute, filter with absorbent cotton, filtrate adds hydrochloric acid 2ml, micro-5-6 minute that boils, and filters, filtrate extracts 3 times with chloroform jolting, each 20-25ml, merges chloroform liquid, volatilizes, residue adds chloroform 1ml makes dissolving, as need testing solution;
(12) preparation of reference substance solution: get control medicinal material 2g Radix Ophiopogonis, be made in the same way of reference substance solution;
(13) thin layer chromatography test: draw reference substance solution 5-10 μ l, need testing solution 5-10 μ l, put respectively in same with sodium carboxymethyl cellulose be adhesive silica gel g thin-layer plate on, take volume ratio as the chloroform-acetone of 3-5:1 be developing solvent, launch, take out, dry, spray with volumetric concentration be 10% sulphuric acid-alcoholic solution, at 100-110 DEG C of temperature, be heated to spot development clear; In test sample chromatograph, on the position corresponding to reference substance solution chromatograph, the speckle (Fig. 1) of aobvious same color,
The need testing solution that reference substance solution, embodiment 1, embodiment 2, embodiment 3 are obtained is followed successively by from left to right in Fig. 1;
(2) to the qualification of the Radix Stemonae:
(21) preparation of need testing solution: get this product 10g, adds ethanol and 1ml hydrochloric acid that 20ml volumetric concentration is 90%, puts reflux 20-25 minute in water-bath, placement is spent the night, and filters, filtrate evaporate to dryness, the residue 30-35ml that adds water makes dissolving, add more than strong ammonia solution adjust pH to 10, extract 3 times with chloroform jolting, each 25ml, merge chloroform liquid, evaporate to dryness, it is that 90% ethanol makes dissolving, as need testing solution that residue adds 1ml volumetric concentration;
(22) preparation of reference substance solution: get Radix Stemonae control medicinal material 3g, be made in the same way of reference substance solution;
(23) preparation of negative control solution: get medical material 3g except the Radix Stemonae, be made in the same way of negative control solution;
(24) thin layer chromatography test: draw reference substance solution 5-10 μ l, reference substance solution 5-10 μ l, negative control solution 5-10 μ l, put respectively in same with sodium carboxymethyl cellulose be adhesive silica gel g thin-layer plate on, take volume ratio as 8-10:1 chloroform-methanol be developing solvent, lamellae put presaturation 30-40 minute in chromatography cylinder, launch, take out, dry, spray to improve bismuth potassium iodide test solution; In test sample chromatograph, on the position corresponding to reference substance solution chromatograph, aobvious identical orange red speckle (Fig. 2),
The need testing solution that negative control solution, reference substance solution, embodiment 1, embodiment 2, embodiment 3 are obtained is followed successively by from left to right in Fig. 2;
(3) to the qualification of Cortex Moutan:
(31) preparation of need testing solution: get this product 10g, add water 50-60ml, chloroform 30-35ml, put reflux 30-35 minute in water-bath, let cool, be transferred in separatory funnel, divide and get chloroform liquid, the jolting of water layer chloroform extracts 2 times, each 30ml, merge chloroform liquid, less than 20 DEG C volatilize, residue adds methanol 1ml immediately makes dissolving, as need testing solution;
(32) preparation of reference substance solution: get paeonol reference substance 3g, adds acetone and makes the solution of every 1ml containing 1mg, product solution in contrast;
(33) preparation of negative control solution: get medical material 3g except Cortex Moutan, the method for same step (31) makes negative control;
(34) thin layer chromatography test: draw reference substance solution 5-10 μ l, need testing solution 5-10 μ l, negative control solution 5-10 μ l, put respectively in same with sodium carboxymethyl cellulose be adhesive silica gel g thin-layer plate on, take volume ratio as the cyclohexane-ethyl acetate of 3-4:1 be developing solvent, launch, take out, dry, spray with the acid 5% ferric chloride alcoholic solution of hydrochloric acid, be heated to spot development clear; In test sample chromatograph, on the position corresponding to reference substance solution chromatograph, the speckle (Fig. 3) of aobvious same color,
The need testing solution that reference substance solution, negative control solution, embodiment 1, embodiment 2, embodiment 3 are obtained is followed successively by from left to right in Fig. 3.
Therapeutic effect
1, physical data
Statistics accept for medical treatment out-patient 80 example, age 25-35 year 20 example, 35-50 year 45 example, 50-70 year 15 example.
2, Therapeutic Method
Boiled water is taken after being infused in hot water or decoction, and once a bag, twice on the one 7 days is a course for the treatment of, takes 3 courses for the treatment of.
3, efficacy assessment standard
Effective: through treatment throat transference cure;
Take a turn for the better: throat remission, pain reduces;
Invalid: do not reach improvement standard, symptom is without alleviation.
4, therapeutic outcome
As seen from the above table, the pharyngitis tea that the present invention obtains, does not have side effects, and after taking three courses for the treatment of, obvious effective rate is 57.5%, and improvement rate is 37.5%, and total effective rate is 95%.
Claims (6)
1. a pharyngitis tea, is characterized in that: be made up of following component by weight: Radix Stemonae 5-10 part, Cortex Moutan 5-10 part, Radix Ophiopogonis 5-10 part, Flos Farfarae 5-10 part processed, Semen Oroxyli 5-10 part, Radix Isatidis 10-20 part, Fructus Canarii 5-10 part, Fructus Momordicae 10-20 part.
2. the preparation method of a kind of pharyngitis tea according to claim 1, it is characterized in that: by above-mentioned weight portion, take Radix Stemonae, Cortex Moutan, Radix Ophiopogonis, Flos Farfarae processed, Semen Oroxyli, Radix Isatidis, Fructus Canarii and Fructus Momordicae, pulverize respectively, 80-120 mesh sieve is crossed, after subpackage pack and get final product after mix homogeneously.
3. the authentication method of a kind of pharyngitis tea according to claim 1, is characterized in that: comprise the following steps:
(1) to the qualification of Radix Ophiopogonis:
(11) preparation of need testing solution: get test sample, add water 40-45min, supersound process, filter with absorbent cotton, filtrate adds hydrochloric acid 2ml, micro-5-6 minute that boils, and filters, filtrate extracts 3 times with chloroform jolting, each 20-25ml, merges chloroform liquid, volatilizes, residue adds chloroform 1ml makes dissolving, as need testing solution;
(12) preparation of reference substance solution: get control medicinal material Radix Ophiopogonis, be made in the same way of reference substance solution;
(13) thin layer chromatography test: draw reference substance solution 5-10 μ l, need testing solution 5-10 μ l, put respectively in same with sodium carboxymethyl cellulose be adhesive silica gel g thin-layer plate on, take volume ratio as the chloroform-acetone of 3-5:1 be developing solvent, launch, take out, dry, spray with volumetric concentration be 10% sulphuric acid-alcoholic solution, at 100-110 DEG C of temperature, be heated to spot development clear; In test sample chromatograph, on the position corresponding to reference substance solution chromatograph, the speckle of aobvious same color;
(2) to the qualification of the Radix Stemonae:
(21) preparation of need testing solution: get test sample, adds ethanol and 1ml hydrochloric acid that 20ml volumetric concentration is 90%, reflux, placement is spent the night, and filters, filtrate evaporate to dryness, the residue 30-35ml that adds water makes dissolving, add more than strong ammonia solution adjust pH to 10, extract 3 times with chloroform jolting, each 25ml, merge chloroform liquid, evaporate to dryness, it is that 90% ethanol makes dissolving, as need testing solution that residue adds 1ml volumetric concentration;
(22) preparation of reference substance solution: get Radix Stemonae control medicinal material, be made in the same way of reference substance solution;
(23) preparation of negative control solution: get medical material except the Radix Stemonae, be made in the same way of negative control solution;
(24) thin layer chromatography test: draw reference substance solution 5-10 μ l, reference substance solution 5-10 μ l, negative control solution 5-10 μ l, put respectively in same with sodium carboxymethyl cellulose be adhesive silica gel g thin-layer plate on, take volume ratio as 8-10:1 chloroform-methanol be developing solvent, lamellae put presaturation 30-40 minute in chromatography cylinder, launch, take out, dry, spray to improve bismuth potassium iodide test solution; In test sample chromatograph, on the position corresponding to reference substance solution chromatograph, aobvious identical orange red speckle;
(3) to the qualification of Cortex Moutan:
(31) preparation of need testing solution: get test sample, add water 50-60ml, chloroform 30-35ml, reflux, let cool, be transferred in separatory funnel, divide and get chloroform liquid, the jolting of water layer chloroform extracts 2 times, each 30ml, merge chloroform liquid, less than 20 DEG C volatilize, residue adds methanol 1ml immediately makes dissolving, as need testing solution;
(32) preparation of reference substance solution: get paeonol reference substance, adds acetone and makes the solution of every 1ml containing 1mg, product solution in contrast;
(33) preparation of negative control solution: get medical material except Cortex Moutan, the method for same step (31) makes negative control;
(34) thin layer chromatography test: draw reference substance solution 5-10 μ l, need testing solution 5-10 μ l, negative control solution 5-10 μ l, put respectively in same with sodium carboxymethyl cellulose be adhesive silica gel g thin-layer plate on, take volume ratio as the cyclohexane-ethyl acetate of 3-4:1 be developing solvent, launch, take out, dry, spray with the acid 5% ferric chloride alcoholic solution of hydrochloric acid, be heated to spot development clear; In test sample chromatograph, on the position corresponding to reference substance solution chromatograph, the speckle of aobvious same color.
4. the authentication method of a kind of pharyngitis tea according to claim 1, is characterized in that: in described step (11), ultrasonic time is 20-25 minute.
5. the authentication method of a kind of pharyngitis tea according to claim 1, is characterized in that: in described step (21), reflux process is carried out in a water bath, and the time is 20-25 minute.
6. the authentication method of a kind of pharyngitis tea according to claim 1, is characterized in that: in described step (31), reflux process is carried out in a water bath, and the time is 30-35 minute.
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| CN108426955A (en) * | 2018-03-11 | 2018-08-21 | 安徽省食品药品检验研究院 | The discrimination method of tuber of stemona genunie medicinal materials |
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