CN105104890A - Composition for promoting meiotic resumption of oocytes of sows and application of composition - Google Patents
Composition for promoting meiotic resumption of oocytes of sows and application of composition Download PDFInfo
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- CN105104890A CN105104890A CN201510624013.0A CN201510624013A CN105104890A CN 105104890 A CN105104890 A CN 105104890A CN 201510624013 A CN201510624013 A CN 201510624013A CN 105104890 A CN105104890 A CN 105104890A
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Abstract
The invention discloses a composition for promoting meiotic resumption of oocytes of sows and application of the composition. The composition comprises vectors and extracts of ajuga forrestii, oregano, horseradish, cinnamon, rosemary, dunaliella and rhoeo discolor. The composition and the application have the advantages that meiotic resumption of the oocytes of the sows can be promoted, the quality of the oocytes can be improved, the reproductive performance of the sows can be improved, the reproductive lives of the sows can be prolonged, the composition is favorable for activating c-mos and AMPK (adenosine monophosphate activated protein kinase), accordingly, starting of the meiotic resumption of the oocytes of the sows can be promoted, the quality of the oocytes of the sows can be improved, the conception rates and the pregnancy proportions of the sows can be increased, the reproductive health of the sows can be promoted, the immunity and health conditions of the sows can be improved, and the service lives of the sows can be prolonged; the composition can be added into feed for the sows, so that the quantities of eliminated sows among reservation sows and adult sows due to poor oocyte quality of the sows can be greatly reduced, the sow elimination rate can be lowered, and the service lives of high-parity sows can be prolonged; the costs of pig farms can be reduced, and the productivity of the pig farms can be improved.
Description
Technical field
The present invention relates to animal feed additive technical field, particularly relate to and a kind ofly promote the composition that sow Oocyte Meiosis is recovered and application.
Background technology
In China's intensive pig production production process, the sow of 50% only provides 30-40 head weanling pig to be just eliminated, and seriously constrains the sustainable development on pig farm.Improve number born of sow and will significantly improve sow reproductive efficiency, the raising of litter size depends on embryo and survives the growth with fetus subsequently, and embryo survives and determined by Oocyte quality.
The growth of egg mother cell starts from embryonic period, embryonic phase, and after birth, egg mother cell secular stagnation is at germ-vesicle phase (GV).After sexal maturity, Oocyte Meiosis is recovered, and germinal vesicle breakdown (GVBD) and first polar body are discharged, and complete initial meiosis.Research shows MAPK (MAPK), in the egg mother cell GV phase complete non-phosphorylating (i.e. non-activity), phosphorylation is started when GVBD, peak at initial meiosis mid-term (MI) and maintain high level until MII (MII), MAPK activity declines when after fertilization Pronucleus formation, and in mitosis, do not have this phenomenon.MOS protein kinase (c-mos proto-oncogene encodes) is MAPK upstream kinases exclusive in vertebrate germ cells.C-mosmRNA is translated in Oocyte Maturation Process, causes the cascade activation of MAPK.MOS is that MPF, GVBD activation institute is required, is also that first polar body formation and MII phase MPF activity acquisition institute are required.
The research that current concerned plant extract activates egg mother cell MOS and MAKP aspect has no report,
Summary of the invention
The present invention is in order to solve above-mentioned production problem, and open one can promote that sow Oocyte Meiosis is recovered, and carries Oocyte quality, improves the plant extracts combination of rate of fertilization and raising nest litter size.Can promote that Oocyte Meiosis is recovered, improve Oocyte quality, increase rate of fertilization, pregnancy rate and parturition rate, reduce sow mortality, improve sow reproductive performance, extend sow service life.
The present invention adopts following technical scheme:
The composition that recovers of promotion sow Oocyte Meiosis of the present invention is careless by only dysentery grass, wild marjoram, horseradish, Chinese cassia tree, rosemary, salt algae and the evergreen extract of purple and carrier form.
The proportioning of described composition is as follows: only dysentery grass extract addition is 5-8wt%, wild marjoram grass extract addition is 4-6wt%, horseradish extract addition is 0.5wt%, cinnamomum cassia extract addition is 2wt%, Rosmarinus officinalis extract addition is 1-3wt%, and algae extrats addition is 1-2.5wt%, and purple Rohdea japonica Roth extract addition is 2-5wt%, remainder is carrier, and carrier is cornstarch.
The preparation method of described plant extracts is as follows: above-mentioned plant or plant leaf are placed in 60 DEG C of drying in oven respectively, crosses 40 mesh sieves, seal for subsequent use with plant pulverizer after pulverizing.Adopt ethanol cold soaking extraction method.Typical process flow is raw material → alcohol extract → concentrated → medicinal extract → spraying dry → pulverizing, mixing, packaging → product.
The composition of described composition comprises carvacrol, cinnamic acid, australene, salt algae element and delphin.
Composition of the present invention can be used for promoting that sow Oocyte Meiosis is recovered.
Composition of the present invention can reduce sow mortality, improves sow reproductive performance, extends sow service life.Composition of the present invention is when applying, and be added in pig feed, addition is 0.5wt ‰.
Good effect of the present invention is as follows:
The present invention in vitro on egg mother cell and sow through checking and test repeatedly, find that combined plant extract effectively can promote that sow egg mother cell c-mosmRNA expresses, and then promote MAKP activation; And determine to add in diet that the only dysentery grass of suitable proportion, wild marjoram are careless, maiotic recovery that horseradish, Chinese cassia tree, rosemary, salt algae and purple Rohdea japonica Roth extract facilitate egg mother cell largely, improve Oocyte quality, improve the feed intake of sow, immunity of organisms and health status simultaneously, improve the reproductive performance of sow.Composition of the present invention recovers and quality for promotion sow Oocyte Meiosis, safeguards that sow breeding achievement and reproductive life have very important significance.
By plant extracts combination, the present invention promotes that sow Oocyte Meiosis is recovered, improve Oocyte quality, improve sow breeding achievement and reproductive life, the main composition of combined plant extract is beneficial to the activation of c-mos, activate AMPK, thus promote the startup that sow Oocyte Meiosis is recovered, improve sow Oocyte quality, improve the ratio of conception rate and sow pregnancy, sow healthy reproduction can be promoted simultaneously, improve sow immunocompetence and health status, extend sow service life.By adding plant extracts of the present invention combination in Sow Diet, replacement gilt and sow being greatly reduced because of the sow quantity that Oocyte quality is not high and superseded, reduces the mortality of sow, also can extend the service life of high parity sow.The cost greatly reducing pig farm drops into, and improves the productivity of plant.
Accompanying drawing explanation
Fig. 1: plant extracts combination promotes porcine oocytes meiosis representative pictures.
In figure, A is typical germ-vesicle phase (GV) intracellular appearance; In figure, B is the intracellular appearance of (GVBD) after typical germinal vesicle breakdown.
Fig. 2: plant extracts combination promotes porcine oocytes germinal vesicle breakdown (GVBD).
Note: above numerical value is average ± standard deviation; Represent that difference is not remarkable containing same letter, different lowercase alphabet shows significant difference, P<0.05.
Fig. 3: plant extracts combination promotes that in-vitro maturity of porcine oocytes reaches the MII phase.
Note: above numerical value is average ± standard deviation; Represent that difference is not remarkable containing same letter, different lowercase alphabet shows significant difference, P<0.05.
Detailed description of the invention
The following examples describe in further detail of the present invention.
Embodiment 1
The composition that recovers of promotion sow Oocyte Meiosis of the present invention is careless by only dysentery grass, wild marjoram, horseradish, Chinese cassia tree, rosemary, salt algae and the evergreen extract of purple form.
The proportioning of described composition is as follows: only dysentery grass extract addition is 5%, wild marjoram grass extract addition is 6%, horseradish extract addition is 0.5%, cinnamomum cassia extract addition is 2%, Rosmarinus officinalis extract addition is 1%, and algae extrats addition is 2.5%, and purple Rohdea japonica Roth extract addition is 2%, remainder is carrier, and carrier is cornstarch.
Embodiment 2
The composition that recovers of promotion sow Oocyte Meiosis of the present invention is careless by only dysentery grass, wild marjoram, horseradish, Chinese cassia tree, rosemary, salt algae and the evergreen extract of purple form.
The proportioning of described composition is as follows: only dysentery grass extract addition is 8%, wild marjoram grass extract addition is 4%, horseradish extract addition is 0.5%, cinnamomum cassia extract addition is 2%, Rosmarinus officinalis extract addition is 3%, and algae extrats addition is 1%, and purple Rohdea japonica Roth extract addition is 5%, remainder is carrier, and carrier is cornstarch.
Embodiment 3
The composition that recovers of promotion sow Oocyte Meiosis of the present invention is careless by only dysentery grass, wild marjoram, horseradish, Chinese cassia tree, rosemary, salt algae and the evergreen extract of purple form.
The proportioning of described composition is as follows: only dysentery grass extract addition is 7%, wild marjoram grass extract addition is 5%, horseradish extract addition is 0.5%, cinnamomum cassia extract addition is 2%, Rosmarinus officinalis extract addition is 2%, and algae extrats addition is 1.5%, and purple Rohdea japonica Roth extract addition is 4%, remainder is carrier, and carrier is cornstarch.
In order to verify effect of the present invention, this invention first in vitro on cell chulture research find that it has the effect promoting that Oocyte Meiosis is recovered, and further by the composition of embodiment 3 in the selected swine farms Preliminary Applications such as Guangxi, Sichuan, effect shows below:
Embodiment 4
In vitro culture porcine oocytes finds, the combination of this plant extracts significantly promotes porcine oocytes Meiotic resumption (Fig. 1), reach GVBD ratio plant extracts combined treatment group and only dysentery grass extract-treated groups be significantly higher than control group (Fig. 2); And the ratio plant extracts combined treatment group reaching MII phase maturation is significantly higher than only dysentery grass extract-treated groups and control group (Fig. 3), illustrate that the combination of this plant extracts has the effect promoting Oocyte Meiosis recovery and maturation in vitro, and effect is better than single only dysentery grass extract.
Embodiment 5
This invention product of 0.5 ‰ is added by grow up in standby (corn-soybean meal diet) in sow feed, sow breeding achievement improves about 8-15% mutually than contrast group, greatly reduce sow in the standby phase with plant by the mortality of phase, more obvious to the effect on the poor pig farm of rearing conditions.
This invention of table 1 is to the effect table (data from 3 pig farms, n=165) of replacement gilt breeding achievement
Embodiment 6
By in adult female Landrace diet (corn-soybean meal diet) add this invention product of 0.5 ‰, sow breeding achievement improves about 9-16% mutually than contrast group, significantly reduce sow kind by the mortality of phase, more obvious to the effect on the poor pig farm of rearing conditions;
This invention of table 2 shows (data come from 3 pig farms, n=247) in adult kind with the performance fruit that is multiplied into after use on sow
Embodiment 7
By this invention with 0.5 ‰ ratio be used for 6 tire sows, the service life of sow can be extended, reduce the sow that causes because of Oocyte quality superseded.
Application result shows: this invention facilitates the growth of sow egg mother cell, improves sow health status and immunologic function, improves sow Oocyte quality, adds service life and the reproductive life of sow, reduces the production cost of plant.
Although illustrate and describe embodiments of the invention, for the ordinary skill in the art, be appreciated that and can carry out multiple change, amendment, replacement and modification to these embodiments without departing from the principles and spirit of the present invention, scope of the present invention is by claims and equivalents thereof.
Claims (8)
1. promote to it is characterized in that the composition that sow Oocyte Meiosis is recovered: described composition is careless by only dysentery grass, wild marjoram, horseradish, Chinese cassia tree, rosemary, salt algae and the evergreen extract of purple and carrier form.
2. the composition promoting the recovery of sow Oocyte Meiosis as claimed in claim 1, it is characterized in that: the proportioning of described composition is as follows: only dysentery grass extract addition is 5-8wt%, wild marjoram grass extract addition is 4-6wt%, horseradish extract addition is 0.5wt%, cinnamomum cassia extract addition is 2wt%, Rosmarinus officinalis extract addition is 1-3wt%, algae extrats addition is 1-2.5wt%, purple Rohdea japonica Roth extract addition is 2-5wt%, remainder is carrier, and carrier is cornstarch.
3. the composition promoting the recovery of sow Oocyte Meiosis as claimed in claim 1 or 2, it is characterized in that: the preparation method of described plant extracts is as follows: above-mentioned plant or plant leaf are placed in 60 DEG C of drying in oven respectively, 40 mesh sieves are crossed after pulverizing with plant pulverizer, seal for subsequent use, adopt ethanol cold soaking extraction method, typical process flow is raw material → alcohol extract → concentrated → medicinal extract → spraying dry → pulverizing, mixing, packaging → product.
4. the composition promoting the recovery of sow Oocyte Meiosis as claimed in claim 1 or 2, is characterized in that: the composition of described composition comprises carvacrol, cinnamic acid, australene, salt algae element and delphin.
5. the composition as described in any one of claim 1-4 is promoting the application that sow Oocyte Meiosis is recovered.
6. apply as claimed in claim 5, it is characterized in that: composition of the present invention is when applying, and be added in pig feed, addition is 0.5wt ‰.
7. the composition as described in any one of claim 1-4, in reduction sow mortality, improves sow reproductive performance, extends the application in sow service life.
8. apply as claimed in claim 7, it is characterized in that: composition of the present invention is when applying, and be added in pig feed, addition is 0.5wt ‰.
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| CN201510624013.0A CN105104890B (en) | 2015-09-25 | 2015-09-25 | It is a kind of promote sow Oocyte Meiosis restore composition and application |
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| CN201510624013.0A CN105104890B (en) | 2015-09-25 | 2015-09-25 | It is a kind of promote sow Oocyte Meiosis restore composition and application |
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| CN105104890B CN105104890B (en) | 2018-11-16 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107574144A (en) * | 2017-10-20 | 2018-01-12 | 中国农业大学 | A kind of in-vitro maturity of porcine oocytes nutrient solution for adding carnosic acid and its application |
| CN107603944A (en) * | 2017-10-20 | 2018-01-19 | 中国农业大学 | A kind of oocyte in vitro maturation culture solution for adding Rosmarinic acid and its application |
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| JP2003245044A (en) * | 2001-12-21 | 2003-09-02 | Kyowa Hakko Kogyo Co Ltd | Feed for klotho-modified homoanimal and klotho gene knocked-out homoanimal |
| CN101163521A (en) * | 2005-04-21 | 2008-04-16 | 德索尔有限公司 | Method for improving the fertility of animals |
| CN104740545A (en) * | 2013-12-25 | 2015-07-01 | 卢国政 | Traditional Chinese medicine for improving farrowing rate of superior sows |
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2015
- 2015-09-25 CN CN201510624013.0A patent/CN105104890B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2003245044A (en) * | 2001-12-21 | 2003-09-02 | Kyowa Hakko Kogyo Co Ltd | Feed for klotho-modified homoanimal and klotho gene knocked-out homoanimal |
| CN101163521A (en) * | 2005-04-21 | 2008-04-16 | 德索尔有限公司 | Method for improving the fertility of animals |
| CN104740545A (en) * | 2013-12-25 | 2015-07-01 | 卢国政 | Traditional Chinese medicine for improving farrowing rate of superior sows |
Non-Patent Citations (2)
| Title |
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| 范衡宇等: "MAPK信号通路在卵母细胞减数分裂中的作用", 《科学通报》 * |
| 金立志: "植物提取物添加剂在动物营养中的应用及其机制的研究进展", 《动物营养学报》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107574144A (en) * | 2017-10-20 | 2018-01-12 | 中国农业大学 | A kind of in-vitro maturity of porcine oocytes nutrient solution for adding carnosic acid and its application |
| CN107603944A (en) * | 2017-10-20 | 2018-01-19 | 中国农业大学 | A kind of oocyte in vitro maturation culture solution for adding Rosmarinic acid and its application |
| CN107603944B (en) * | 2017-10-20 | 2020-10-02 | 中国农业大学 | Rosmarinic acid-added oocyte in-vitro maturation culture solution and application thereof |
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| CN105104890B (en) | 2018-11-16 |
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