CN105104890B - It is a kind of promote sow Oocyte Meiosis restore composition and application - Google Patents
It is a kind of promote sow Oocyte Meiosis restore composition and application Download PDFInfo
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- CN105104890B CN105104890B CN201510624013.0A CN201510624013A CN105104890B CN 105104890 B CN105104890 B CN 105104890B CN 201510624013 A CN201510624013 A CN 201510624013A CN 105104890 B CN105104890 B CN 105104890B
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Abstract
The invention discloses a kind of composition that promotion sow Oocyte Meiosis is restored and applications.The composition is made of stop dysentery grass, wild marjoram grass, horseradish, cortex cinnamomi, rosemary, salt algae and purple evergreen extract and carrier.The present invention can promote sow Oocyte Meiosis to restore, improve Oocyte quality, improve sow breeding achievement and reproductive life, conducive to the activation of c-mos, AMPK is activated, thus the starting for promoting sow Oocyte Meiosis to restore, it improves sow Oocyte quality, improve conception rate and the ratio of sow pregnancy, it can promote sow healthy reproduction simultaneously, improve sow immunocompetence and health status, extend sow service life.Composition of the invention is added in Sow Diet makes replacement gilt and adult sow because the not high and superseded sow quantity of Oocyte quality greatly reduces, and reduces the mortality of sow, extends the service life of high parity sow.The cost for reducing pig farm improves the productivity of farm.
Description
Technical field
The present invention relates to animal feed additive technical field more particularly to a kind of promotion sow Oocyte Meiosis
The composition of recovery and application.
Background technique
In China's intensive pig production production process, 50% sow only provides 30-40 weanling pigs and is just eliminated, seriously
Constrain the sustainable development on pig farm.Sow reproductive efficiency will be significantly improved by improving number born of sow, the raising of litter size according to
Rely and survives the development with subsequent fetus in embryo, and embryo survives and is determined by Oocyte quality.
The development of egg mother cell starts from embryonic period, embryonic phase, and egg mother cell secular stagnation is at germ-vesicle phase (GV) after birth.Sexal maturity
Afterwards, Oocyte Meiosis is restored, and initial meiosis is completed in germinal vesicle breakdown (GVBD) and first polar body discharge.It grinds
Study carefully and show mitogen-activated protein kinase (MAPK), in egg mother cell GV phase complete non-phosphorylating (i.e. inactive), in GVBD
Start phosphorylation, peak in initial meiosis mid-term (MI) and maintains high level until in second meiotic division
Phase (MII), MAPK activity decline in after fertilization Pronucleus formation, and without this phenomenon in mitosis.MOS albumen swashs
Enzyme (c-mos proto-oncogene coding), is MAPK upstream kinases exclusive in vertebrate germ cells.C-mos mRNA is in ovum mother
It is translated in cell mature process, causes the cascade activation of MAPK.MOS is MPF, and GVBD activates required and first polar body
It is formed and MII phase MPF activity acquisition institute is required.
The research in terms of related plant extracts activation egg mother cell MOS and MAKP has not been reported at present,
Summary of the invention
The present invention in order to solve the problems, such as above-mentioned production, disclose it is a kind of sow Oocyte Meiosis can be promoted to restore, mention
Oocyte quality improves rate of fertilization and improves the plant extracts combination of nest litter size.It can promote egg mother cell subtrahend point
Recovery is split, Oocyte quality is improved, increases rate of fertilization, pregnancy rate and parturition rate, reduces sow mortality, improves sow breeding
Performance extends sow service life.
The present invention adopts the following technical scheme that：
The composition that promotion sow Oocyte Meiosis of the invention is restored is by stop dysentery grass, wild marjoram grass, horseradish, meat
Osmanthus, rosemary, salt algae and purple evergreen extract and carrier composition.
The proportion of the composition is as follows：Stop dysentery grass extract additive amount is 5-8wt%, wild marjoram grass extract additive amount
For 4-6wt%, horseradish extract additive amount is 0.5wt%, and cinnamomum cassia extract additive amount is 2wt%, Rosmarinus officinalis extract addition
Amount is 1-3wt%, and algae extrats additive amount is 1-2.5wt%, and purple Rohdea japonica Roth extract additive amount is 2-5wt%, and remainder is
Carrier, carrier are cornstarch.
The preparation method of the plant extracts is as follows：Above-mentioned plant or plant leaf are respectively placed in 60 DEG C of baking ovens and dried
It is dry, 40 meshes are smashed it through with plant pulverizer, are sealed spare.Using ethyl alcohol cold soaking extraction method.Typical process flow is raw material
→ ethyl alcohol extraction → concentration → medicinal extract → spray drying → crushing, mixing, packaging → product.
The ingredient of the composition includes carvacrol, cinnamic acid, australene, salt algae element and delphin.
Composition of the invention can be used for that sow Oocyte Meiosis is promoted to restore.
Composition of the invention can reduce sow mortality, improve sow reproductive performance, extend sow service life.This
For the composition of invention in use, being added into pannage, additive amount is 0.5wt ‰.
The positive effect of the present invention is as follows：
The present invention has found that combined plant extract can have in vitro on egg mother cell and sow by verifying repeatedly with test
Effect promotes sow egg mother cell c-mos mRNA expression, and then promotes MAKP activation；And suitable proportion is added in determination in diet
Stop dysentery grass, wild marjoram grass, horseradish, cortex cinnamomi, rosemary, salt algae and purple Rohdea japonica Roth extract largely promote egg mother cell
Meiosis recovery, improve Oocyte quality, while improving the feed intake of sow, immunity of organisms and healthy shape
Condition improves the reproductive performance of sow.Composition of the invention is for promoting the recovery of sow Oocyte Meiosis and quality, dimension
Shield sow breeding achievement and reproductive life have very important significance.
The present invention is combined by plant extracts promotes sow Oocyte Meiosis to restore, and improves egg mother cell matter
Amount improves sow breeding achievement and reproductive life, and the main ingredient of combined plant extract is conducive to the activation of c-mos, activation
AMPK, thus promote sow Oocyte Meiosis restore starting, improve sow Oocyte quality, improve conception rate and
The ratio of sow pregnancy, while sow healthy reproduction can be promoted, improve sow immunocompetence and health status, extends sow and use
The time limit.By adding plant extracts combination of the invention in Sow Diet, make replacement gilt and adult sow because ovum is female
The not high and superseded sow quantity of cell quality greatly reduces, and reduces the mortality of sow, can also extend high parity sow
Service life.The cost input on pig farm is greatly reduced, the productivity of farm is improved.
Detailed description of the invention
Fig. 1：Plant extracts combination promotes porcine oocytes meiosis representative pictures.
A is typical germ-vesicle phase (GV) intracellular appearance in figure；B is after typical germinal vesicle breakdown (GVBD) in figure
Intracellular appearance.
Fig. 2：Plant extracts combination promotes porcine oocytes germinal vesicle breakdown (GVBD).
Note：The above numerical value is average ± standard deviation；Indicate that difference is not significant containing same letter, different lowercase alphabets
Show significant difference, P<0.05.
Fig. 3：Plant extracts combination promotes in-vitro maturity of porcine oocytes to reach the MII phase.
Note：The above numerical value is average ± standard deviation；Indicate that difference is not significant containing same letter, different lowercase alphabets
Show significant difference, P<0.05.
Specific embodiment
The following examples are a further detailed description of the invention.
Embodiment 1
The composition that promotion sow Oocyte Meiosis of the invention is restored is by stop dysentery grass, wild marjoram grass, horseradish, meat
Osmanthus, rosemary, salt algae and purple evergreen extract composition.
The proportion of the composition is as follows：Stop dysentery grass extract additive amount is 5%, and wild marjoram grass extract additive amount is
6%, horseradish extract additive amount is 0.5%, and cinnamomum cassia extract additive amount is 2%, and Rosmarinus officinalis extract additive amount is 1%, salt
Algae extract additive amount is 2.5%, and purple Rohdea japonica Roth extract additive amount is 2%, and remainder is carrier, and carrier is cornstarch.
Embodiment 2
The composition that promotion sow Oocyte Meiosis of the invention is restored is by stop dysentery grass, wild marjoram grass, horseradish, meat
Osmanthus, rosemary, salt algae and purple evergreen extract composition.
The proportion of the composition is as follows：Stop dysentery grass extract additive amount is 8%, and wild marjoram grass extract additive amount is
4%, horseradish extract additive amount is 0.5%, and cinnamomum cassia extract additive amount is 2%, and Rosmarinus officinalis extract additive amount is 3%, salt
Algae extract additive amount is 1%, and purple Rohdea japonica Roth extract additive amount is 5%, and remainder is carrier, and carrier is cornstarch.
Embodiment 3
The composition that promotion sow Oocyte Meiosis of the invention is restored is by stop dysentery grass, wild marjoram grass, horseradish, meat
Osmanthus, rosemary, salt algae and purple evergreen extract composition.
The proportion of the composition is as follows：Stop dysentery grass extract additive amount is 7%, and wild marjoram grass extract additive amount is
5%, horseradish extract additive amount is 0.5%, and cinnamomum cassia extract additive amount is 2%, and Rosmarinus officinalis extract additive amount is 2%, salt
Algae extract additive amount is 1.5%, and purple Rohdea japonica Roth extract additive amount is 4%, and remainder is carrier, and carrier is cornstarch.
In order to verify application effect of the invention, the invention first in vitro in cell culture research discovery its have and promote
The effect that Oocyte Meiosis is restored, and it is further that the composition of embodiment 3 is preliminary in selected swine farms such as Guangxi, Sichuan
Using effect shows as follows：
Embodiment 4
The discovery of in vitro culture porcine oocytes, the combination of this plant extracts remarkably promote porcine oocytes Meiotic resumption
(Fig. 1), the ratio plant extracts combined treatment group and stop dysentery grass extract-treated groups for reaching GVBD are significantly higher than control group (figure
2)；And the ratio plant extracts combined treatment group for reaching MII phase maturation is significantly higher than stop dysentery grass extract-treated groups and control
Group (Fig. 3) illustrates that the combination of this plant extracts has the function of promoting Oocyte Meiosis recovery and maturation in vitro, and makees
With better than single stop dysentery grass extract.
Embodiment 5
By growing up the invention product of (corn-soybean meal diet) addition 0.5 ‰ in sow feed in standby, sow is numerous
It grows achievement and mutually improves 8-15% or so than control group, the mortality that sow uses the phase in standby phase and kind is greatly reduced, to feeding
The effect on the poor pig farm of the condition of supporting is more obvious.
Effect table of the invention of table 1 to replacement gilt breeding achievement (data come from 3 pig farms, n=165)
Embodiment 6
By the invention product of (corn-soybean meal diet) addition 0.5 ‰ in adult female Landrace diet, sow is numerous
Grow achievement and mutually improve 9-16% or so than control group, significantly reduce sow kind with the mortality of phase, to rearing conditions compared with
The effect on the pig farm of difference is more obvious；
In adult kind, with the performance fruit table that is multiplied into after use on sow, (data are derived from 3 pig farms, n=to the invention of table 2
247)
Embodiment 7
The invention is used for 6 tire sows with 0.5 ‰ ratio, the service life of sow can be extended, is reduced because ovum is female thin
Sow is eliminated caused by cytoplasm amount.
Using as the result is shown：The invention promotes the development of sow egg mother cell, improves sow health status and is immunized
Function improves sow Oocyte quality, increases the service life and reproductive life of sow, reduces the production of farm
Cost.
It although an embodiment of the present invention has been shown and described, for the ordinary skill in the art, can be with
A variety of variations, modification, replacement can be carried out to these embodiments without departing from the principles and spirit of the present invention by understanding
And modification, the scope of the present invention is defined by the appended.
Claims (1)
1. a kind of composition for promoting sow Oocyte Meiosis to restore, it is characterised in that：The composition is by only
Dysentery grass, wild marjoram grass, horseradish, cortex cinnamomi, rosemary, salt algae and purple evergreen extract and carrier composition,
The proportion of the composition is as follows：Stop dysentery grass extract additive amount is 5-8wt%, and wild marjoram grass extract additive amount is 4-
6wt%, horseradish extract additive amount are 0.5wt%, and cinnamomum cassia extract additive amount is 2wt%, and Rosmarinus officinalis extract additive amount is
1-3wt%, algae extrats additive amount are 1-2.5wt%, and purple Rohdea japonica Roth extract additive amount is 2-5wt%, and remainder is carrier,
Carrier is cornstarch,
The preparation method of the plant extracts is as follows：Above-mentioned plant or plant leaf are respectively placed in 60 DEG C of drying in oven,
40 meshes are smashed it through with plant pulverizer, sealing is spare, and using ethyl alcohol cold soaking extraction method, typical process flow is raw material → second
Alcohol extracting → concentration → medicinal extract → spray drying → crushing, mixing, packaging → product.
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| CN201510624013.0A CN105104890B (en) | 2015-09-25 | 2015-09-25 | It is a kind of promote sow Oocyte Meiosis restore composition and application |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107574144B (en) * | 2017-10-20 | 2020-10-02 | 中国农业大学 | Carnosic acid-added porcine oocyte in-vitro maturation culture solution and application thereof |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107603944B (en) * | 2017-10-20 | 2020-10-02 | 中国农业大学 | Rosmarinic acid-added oocyte in-vitro maturation culture solution and application thereof |
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|---|---|---|---|---|
| JP2003245044A (en) * | 2001-12-21 | 2003-09-02 | Kyowa Hakko Kogyo Co Ltd | Feed for klotho-modified homoanimal and klotho gene knocked-out homoanimal |
| CN101163521A (en) * | 2005-04-21 | 2008-04-16 | 德索尔有限公司 | Method for improving the fertility of animals |
| CN104740545A (en) * | 2013-12-25 | 2015-07-01 | 卢国政 | Traditional Chinese medicine for improving farrowing rate of superior sows |
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2015
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2003245044A (en) * | 2001-12-21 | 2003-09-02 | Kyowa Hakko Kogyo Co Ltd | Feed for klotho-modified homoanimal and klotho gene knocked-out homoanimal |
| CN101163521A (en) * | 2005-04-21 | 2008-04-16 | 德索尔有限公司 | Method for improving the fertility of animals |
| CN104740545A (en) * | 2013-12-25 | 2015-07-01 | 卢国政 | Traditional Chinese medicine for improving farrowing rate of superior sows |
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| MAPK信号通路在卵母细胞减数分裂中的作用;范衡宇等;《科学通报》;20021231;第47卷(第9期);第650-656页 * |
| 植物提取物添加剂在动物营养中的应用及其机制的研究进展;金立志;《动物营养学报》;20101231;第22卷(第5期);第1154-1164页 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107574144B (en) * | 2017-10-20 | 2020-10-02 | 中国农业大学 | Carnosic acid-added porcine oocyte in-vitro maturation culture solution and application thereof |
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