CN104146157A - Preparation method and application method of feed additive containing Melaleuca alternifolia tree oil - Google Patents
Preparation method and application method of feed additive containing Melaleuca alternifolia tree oil Download PDFInfo
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- CN104146157A CN104146157A CN201410442511.9A CN201410442511A CN104146157A CN 104146157 A CN104146157 A CN 104146157A CN 201410442511 A CN201410442511 A CN 201410442511A CN 104146157 A CN104146157 A CN 104146157A
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Abstract
The invention discloses a preparation method and an application method of a feed additive containing Australia tea tree oil (Melaleuca alternifolia tree oil) and an application method of the feed additive fed to some animals. The preparation method comprises the following steps: heating and thermally insulating an essence containing 30-40% of terpinene-4-ol for three times, cooling the essence to the normal temperature; adding tap water and vitamin E into the essence, heating and stirring up, loading into a container, stirring by a high speed shearing machine, bottling and finally obtaining the feed additive. The feed additive is diluted by water according to different proportions to prepare the feed additive dilute solution, and the feed additive dilute solution can be used as the drinking water for new born chickens, or for chickens which are seven-day old, or for weaned pigs, or pigs which have been weaned for 10 days. The animals are fed with the feed additive or the feed raw material additive, so as to improve the immunity of animals and prevent avian influenza, animal influenza and other diseases.
Description
Technical field
The present invention relates to a kind of feed addictive preparation method, relate in particular to a kind of Australia tea oil (narrow leaved tea tree tree oil) feed addictive preparation method and preparing product using method.
Background technology
Australia tea oil is a kind of trade name, and its formal name used at school is narrow leaved tea tree tree oil.Narrow leaved tea tree tree is a kind of bushes that mainly grows in Australian southeastern coast.Australia tea oil is had history in several thousand by aborigines for wound disinfection.Australia's tea oil was successfully listed European Pharmacopoeia in 2000, obtained the approval of the approval of Chinese Bureau of Drugs Supervision and U.S. FDA as food flavor additive.
Through recent studies, find that Australia tea oil can effectively suppress a lot of Causative virus, has very large effect for treatment numerous disease and wound.As bone is ached, burn, flu etc.Due to the excellent specific property of Australia tea tree, in a lot of countries, introduce a fine variety, in Guangdong of China, also there is plantation in the area such as Yunnan.
Along with the reach of science, free radical is considered to cause mankind aging, diseases induced, DNA mutation and the arch-criminal that causes tumour.Some antioxidants of removing free radical are in succession found and have been applied in our daily life, as deep sea fish oil, lycopene, shrimp Celadon element, white profound extra large krill wet goods.Scientist, study in various applicable naturals, find terpineol-4(terpinol-4 in Australian tea oil) there is extremely strong oxidation resistance, through the effort of recent two decades, Australia scientist successfully gathers and processes edible terpineol-4 essential oil at first from the tea tree of Australia.Through U.S. BROWNSWICKLAB, detect, the ability that Australian tea tree ethereal oil is removed free radical is 4 times of white profound extra large krill oil, 30 times of Shi Xia Celadon element, 25 times of lycopene, 200 times of deep sea fish oil.Australia's tea tree ethereal oil not only has extremely strong antioxidation, have the function of killing obstinate virus concurrently simultaneously, live body chicken embryo experiment through Zhejiang Center For Disease Control and Prevention, has proved all avian influenza virus of H1N1, H5N1, H3N2, H7H9 that can kill in chicken embryo.And similarly test, in the world, many places are all underway.
In sum, Australian tea oil (narrow leaved tea tree tree oil) is refined through give secondary on essential oil basis, changed the molecular structure of original essential oil, make it some indexs and changed.With terpineol-4, this is an important indicator of antioxidant, and index is higher, and oxidation resistance is stronger, improves immunity ability stronger.The Australian tea oil of producing for the first time from factory (narrow leaved tea tree tree oil) essential oil, terpineol-4 numerical value the content of manufactured goods is only 30-40%, and contain certain toxicity, be only used in cosmetics, skin care item, a small amount of flavoring essence additive etc., can not directly eat.
The chicken of current China, pig etc. bird, meat, needs within growth period that regular to give antibiotic etc. medicine disease-resistant, and this fundamentally gives people ediblely brings health hazard.People utilize natural food as feed addictive in searching always, join in animal feed, improve thus the immunity of animal, birds flu-preventing, animal flu and other diseases.
Summary of the invention
technical problem:the invention reside in and overcome the deficiencies in the prior art, provide a kind of narrow leaved tea tree to set oily feed addictive preparation method and application.
technical scheme:
With regard to existing Australian tea oil (narrow leaved tea tree tree oil) essential oil, terpineol-4 numerical value the content of its manufactured goods is 30-40%, and secondary refines on this basis, is not adding under the prerequisite of any chemical assistant, reduced toxic component, the terpineol-4 numerical value content of manufactured goods is 60-66%.Essential oil after secondary refines is as animal feed additive and feedstuff additive, and to the immunity that can improve animal after feeding animal, birds flu-preventing, prevents animal flu and other diseases.This numeral of newly making of terpineol-4 (not using any chemical assistant, content 60-66%), has determined that the oxidation resistance of this novel tea oil is greatly higher than health products on market, such as astaxanthin, and Co-Q10 etc.Proved the general level of the health that has greatly improved animal simultaneously.
Concrete scheme is as follows:
Narrow leaved tea tree is set an oily feed addictive preparation method, comprises the following steps:
Step 1: be that the first essential oil extracting of 30-40% packs reactor into as raw material using terpineol-4 content, be filled with nitrogen, keep 0.2kg/CM2-1kg/CM2 pressure in reactor, through three intensifications, insulation, be specially:
1) be warming up to 30-45 ° of C, insulation 50-60 minute;
2) be warming up to 45-70 ° of C, be incubated 60 minutes;
3) heat to 70-85 ° of C, be incubated 60 minutes;
After three times the process of heating finishes, make essential oil be cooled to normal temperature;
Step 2: take out the essential oil processing, add water and vitamin E to mix; Essential oil: water: the ratio of vitamin E is 20:1000:5, after first 90 ° of C of water and vitamin E heating being stirred, adds essential oil, after stirring, is filled into bottle with high-speed shearing machine.
Described water can be pure water, distilled water or running water.
The additive of described step 2 gained is made to dilution:
By additive: water is 1:1000 dilution, as the drinking water that goes out shell chicken;
By additive: water is 2:1000 dilution, as the drinking water that goes out shell chicken after 7 days.
The additive of described step 2 gained is made to dilution:
By additive: water is 1:250 dilution, as the drinking water of small weaning pig;
By additive: water is 1:125 dilution, as the drinking water of wean piggy after 10 days to selling.
beneficial effect:
From plant produced Australian tea oil (narrow leaved tea tree tree oil) essential oil out, the terpineol-4 numerical value content of manufactured goods is 30-40%.Technique of the present invention secondary on special equipment refines, do not adding under the prerequisite of any chemical assistant, terpineol-4 numerical value the content of manufactured goods is 60-66%, and this numeral has determined that the oxidation resistance of this novel tea oil is greatly higher than astaxanthin etc. health products.
The Australian tea oil that the present invention makes (narrow leaved tea tree tree oil) feed addictive, is natural gathering and processing, and acts on animal bodies, can make it to improve immunity, and not contain any toxic and side effect.Thereby concrete use chicken, pig etc. meat, bird fed, with it, containing hormone, containing antibiotic, rely on the extremely strong oxidation resistance of self, greatly improve the immunity of animal, thus can birds flu-preventing, prevention animal flu and other diseases.Improved greatly the general level of the health of animal.
The present invention gives animal as feed addictive or feedstuff additive feeding, has solved Australian tea oil (narrow leaved tea tree tree oil) essential oil never in the direct edible problem of animal.
Accompanying drawing explanation
Fig. 1 is that narrow leaved tea tree is set oily main component, the test data contrast tables such as raw material essential oil, essential oil of the present invention, Australia standard, New Zealand Standard.
Fig. 2 is the comparison diagram that essential oil of the present invention and various antioxidant are driven away free radical ability.
The specific embodiment
In conjunction with test data, the invention will be further described.
1. a narrow leaved tea tree is set in oily feed addictive preparation method step 1 and is: the essential oil that is 30-40% by terpineol-4 content packs reactor into, open nitrogen cylinder, this nitrogen role be to the narrow leaved tea tree tree olein oil in reactor when the chemical reaction, play stabilization.Then pressurization during 0.2kg/CM2-1kg/CM2 pressure, is warming up to 30-45 ° of C in reactor, insulation 50-60 minute; Allow pressure and temperature act on narrow leaved tea tree tree olein oil, make it to occur chemical reaction.Open oil circulating pump simultaneously, allow essential oil constantly circulate in reactor, realize the abundant reaction of essential oil.Be warming up to again 45-70 ° of C insulation 60 minutes; Pressure keeps 0.2kg/CM2-1kg/CM2 pressure, then heats to 70-85 ° of C, is incubated 60 minutes; Still keep 0.2kg/CM2-1kg/CM2 pressure, finally with cooling water, in pancake coil, be cooled to normal temperature.Turn off air compressor machine, turn off nitrogen, stop the circulation of essential oil.In this process, according to narrow leaved tea tree tree oil, there is very strong volatile feature, impurity and unwanted composition in narrow leaved tea tree tree olein oil have been driven away, retain and improve the composition needing, the high anti-oxidation ability that contains that just can our needs of output, can improve the product oil of immunity.Terpineol-4 numerical value content by the essential oil manufactured goods after the secondary refinement of above-mentioned steps gained is 60-66%.
This test data can detect with 9790 type gas chromatographs.
See Fig. 1, from the table of comparisons, can find out that terpineol-4 content in the product that the present invention produces is much better than the raw material essential oil content of tea oil factory, be much better than Australia, Zelanian national standard, this index is to improve the most important index of immunity, is the most important thing.
Concrete implementation example:
1, the essential oil that is 30-40% by terpineol-4 content packs reactor into, is filled with nitrogen, keeps 0.2kg/CM2 pressure in reactor, through three intensifications, insulation, is specially:
1) be warming up to 30 ° of C, be incubated 50 minutes;
2) be warming up to 45 ° of C, be incubated 60 minutes;
3) heat to 70 ° of C, be incubated 60 minutes;
After three times the process of heating finishes, make essential oil be cooled to normal temperature.
2, the essential oil that is 30-40% by terpineol-4 content packs reactor into, is filled with nitrogen, keeps 1kg/CM2 pressure in reactor, through three intensifications, insulation, is specially:
1) be warming up to 45 ° of C, be incubated 60 minutes;
2) be warming up to 70 ° of C, be incubated 60 minutes;
3) heat to 85 ° of C, be incubated 60 minutes;
After three times the process of heating finishes, make essential oil be cooled to normal temperature.
3, the essential oil that is 30-40% by terpineol-4 content packs reactor into, is filled with nitrogen, keeps 0.5kg/CM2 pressure in reactor, through three intensifications, insulation, is specially:
1) be warming up to 40 ° of C, be incubated 55 minutes;
2) be warming up to 60 ° of C, be incubated 60 minutes;
3) heat to 80 ° of C, be incubated 60 minutes;
After three times the process of heating finishes, make essential oil be cooled to normal temperature.
The essential oil that above-mentioned 1-3 specific embodiment is refined, adds running water and vitamin E to mix; Essential oil: running water: the ratio of vitamin E is 20:1000:5, with running water and vitamin E, put into glass beaker, with heating mixer, added thermal agitation, be warming up to after 90 ° of C, add essential oil, after stirring with high-speed shearing machine, allow essential oil: running water: vitamin E fully mixes, then be filled into bottle.
Specifically to raise test case, be illustrated below:
The chicken of 1, take in bird is example
1) take out 27 chickens of 3 days little white feather chickens of shell, weigh and weigh altogether 1209.6 grams (average 44.8 grams).The mixed finished product dilution of additive, vitamin E, water with gained of the present invention; Press the dilution of dilution 1:1000 water, put into tank or water fountain, abdicate shell chicken diet voluntarily; Within 7 days, press afterwards dilution 2:1000 water dilution, put into tank or water fountain, allow chicken diet voluntarily; Within the 28th day, be weighed as average 13365 grams (average 495 grams).Within the 40th day, the average 32400(that weighs is average 1200 grams).Without dead.
2) without additive situation: take out 27 chickens of 3 days little white feather chickens of shell, weigh and weigh altogether 1236.6 grams (average 45.8 grams).Normal feeding antibiotic and Cold proof medicine,, be weighed as 9698 grams (average 421 grams) on the 28th day by 7 days dead 4.Within the 40th day, the 23460(that weighs is average 1020 grams).Dead 4.
By above-mentioned contrast, can find out that the chicken of the additive of taking gained of the present invention is owing to having improved immunity, healthy, body weight is compared and is able to normal increase.The death rate is lower.
The pig of 2, take in domestic animals is example
1) get 5 of small weaning pigs, 71500 grams (average 14300 grams) the mixed finished product dilution of additive, vitamin E, water with gained of the present invention of weighing; Press dilution 1:250 water dilution, put into tank or water fountain, allow small weaning pig diet voluntarily; Within 10 days, press afterwards dilution 1:125 water dilution, put into tank or water fountain by pig diet voluntarily; Within 60 days, weigh 125500 grams (average 25100 grams), within 105 days, weigh 267500 grams (average 53500 grams), within 125 days, weigh 344000 grams (average 68800 grams).Without dead.
2) without additive situation: get 5 of small weaning pigs weigh 75500 grams (average 15100 grams) normally give antibiotic and Cold proof medicine.Within 60 days, weigh 93000 grams (average 23250 grams), within 105 days, weigh 191500 grams (average 47875 grams), within 125 days, weigh 191500 grams (average 63833 grams), dead 1.
By above-mentioned contrast, can find out that the piggy of the additive of taking gained of the present invention is owing to having improved immunity, healthy, body weight is compared and is able to normal increase.The death rate is lower.
Because narrow leaved tea tree tree oil has the characteristic that improves widely immunity, by that analogy, according to animal food habit and feeding method, the additive of this invention gained, according to body weight, the feature of food-intake, after certain ratio is adjusted, can give ox, sheep, duck, goose, pigeon, rabbit etc. feeding animal, equally can obtain good health effect.
Meanwhile, this invention not only can be by the form of aqua, can also be with the form of capsule to animal edible.
Can also and feed mix, with the form of feed, give feeding animal.
The applicable animal range of this invention is extensive, and using method is also varied.
With bird flu test, be illustrated below:
Get Strain (A type H4N4, H7N9, H3N2, H5N4), chicken embryo, GPRBC, MEM maintenance medium, dilution.With the additive of gained of the present invention, do material medicine.
Experiment is established: positive control: with Tamiflu medicine and Experimental agents parallel laboratory test.
100,10,1,0.1EID50 virus consumption contrast (100EID50):: negative control.
By H1N1, H7N9, H3N2, the additive inoculation egg embryo of H5N4 dilution and this present invention gained, finds that according to different proportionings the additive of this present invention gained is to A type bird flu H1N1, H7N9, H3N2, H5N4 has inhibitory action:
(1) concentration that can suppress A type H3N2 influenza virus is completely 1:5,1:10;
(2) concentration that can suppress A type H5N4 influenza virus is completely 1:5;
(3) concentration that can suppress A type H4N4 influenza virus is completely 1:5,1:10;
(4) concentration that can suppress A type H7N9 influenza virus is completely 1:5,1:10.
Fig. 2 is the comparison diagram that additive of the present invention and various antioxidant are driven away free radical ability.Narrow leaved tea tree tree oil is after secondary refines, the contrast of removing free radical abilities with the various active ingredients in nutrient and healthcare products.This shows, additive of the present invention is due to its distinctive strong oxidability, thus it frequently some health products on the market have the ability of stronger removing free radical.
Claims (3)
1. narrow leaved tea tree is set an oily feed addictive preparation method, it is characterized in that comprising the following steps:
Step 1: be that the first essential oil extracting of 30-40% packs reactor into as raw material using terpineol-4 content, be filled with nitrogen, keep 0.2kg/CM2-1kg/CM2 pressure in reactor, through three intensifications, insulation, be specially:
1) be warming up to 30-45 ° of C, insulation 50-60 minute;
2) be warming up to 45-70 ° of C, be incubated 60 minutes;
3) heat to 70-85 ° of C, be incubated 60 minutes;
After three times the process of heating finishes, make essential oil be cooled to normal temperature;
Step 2: take out the essential oil processing, add water and vitamin E to mix; Essential oil: water: the ratio of vitamin E is 20:1000:5, is first heated to 90 ° of C by water and vitamin E, after stirring, adds essential oil, after stirring, is filled into bottle with high-speed shearing machine.
2. a using method for the product of preparation method's output according to claim 1, is characterized in that the additive of gained in described claim 1 step 2 to make dilution:
By additive: water is 1:1000 dilution, as the drinking water that goes out shell chicken;
By additive: water is 2:1000 dilution, as the drinking water that goes out shell chicken after 7 days.
3. a using method for the product of preparation method's output according to claim 1, is characterized in that the additive of gained in described claim 1 step 2 to make dilution:
By additive: water is 1:250 dilution, as the drinking water of small weaning pig;
By additive: water is 1:125 dilution, as the drinking water of wean piggy after 10 days.
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Cited By (10)
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| CN105053532A (en) * | 2015-07-31 | 2015-11-18 | 王�琦 | Natural tea tree oil additive for substituting forage antibiotic, and production technology thereof |
| CN105079118A (en) * | 2015-07-31 | 2015-11-25 | 王�琦 | Natural tea tree oil agent used for improving human immunity and production process thereof |
| CN105795134A (en) * | 2016-03-16 | 2016-07-27 | 王璞 | Preparation and use methods of pure natural animal feed additive based on tea tree oil |
| CN106666103A (en) * | 2017-01-11 | 2017-05-17 | 厦门百度科技开发股份有限公司 | Composite feed additive and feed containing additive |
| CN107981048A (en) * | 2017-12-01 | 2018-05-04 | 王璞 | The method of preparation and use of feed addictive based on tea oil |
| CN110679739A (en) * | 2019-07-26 | 2020-01-14 | 河源澳美农业科技有限公司 | Melaleuca alternifolia (Australia tea tree) tree residue powder biological feed |
| CN110771736A (en) * | 2019-11-01 | 2020-02-11 | 广西壮族自治区林业科学研究院 | Biological feed containing baeckea frutescens residue and cajeput residue and preparation method thereof |
| CN110771732A (en) * | 2019-11-01 | 2020-02-11 | 广西壮族自治区林业科学研究院 | Melaleuca leucadendra residue biological feed additive and preparation method thereof |
| CN110771733A (en) * | 2019-11-01 | 2020-02-11 | 广西壮族自治区林业科学研究院 | Melaleuca leucadendra residue biological feed and preparation method thereof |
| CN114668147A (en) * | 2022-03-25 | 2022-06-28 | 华南农业大学 | Melaleuca alternifolia compound essential oil microemulsion and preparation method thereof |
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105053532A (en) * | 2015-07-31 | 2015-11-18 | 王�琦 | Natural tea tree oil additive for substituting forage antibiotic, and production technology thereof |
| CN105079118A (en) * | 2015-07-31 | 2015-11-25 | 王�琦 | Natural tea tree oil agent used for improving human immunity and production process thereof |
| CN105795134A (en) * | 2016-03-16 | 2016-07-27 | 王璞 | Preparation and use methods of pure natural animal feed additive based on tea tree oil |
| CN106666103A (en) * | 2017-01-11 | 2017-05-17 | 厦门百度科技开发股份有限公司 | Composite feed additive and feed containing additive |
| CN107981048A (en) * | 2017-12-01 | 2018-05-04 | 王璞 | The method of preparation and use of feed addictive based on tea oil |
| CN110679739A (en) * | 2019-07-26 | 2020-01-14 | 河源澳美农业科技有限公司 | Melaleuca alternifolia (Australia tea tree) tree residue powder biological feed |
| CN110771736A (en) * | 2019-11-01 | 2020-02-11 | 广西壮族自治区林业科学研究院 | Biological feed containing baeckea frutescens residue and cajeput residue and preparation method thereof |
| CN110771732A (en) * | 2019-11-01 | 2020-02-11 | 广西壮族自治区林业科学研究院 | Melaleuca leucadendra residue biological feed additive and preparation method thereof |
| CN110771733A (en) * | 2019-11-01 | 2020-02-11 | 广西壮族自治区林业科学研究院 | Melaleuca leucadendra residue biological feed and preparation method thereof |
| CN114668147A (en) * | 2022-03-25 | 2022-06-28 | 华南农业大学 | Melaleuca alternifolia compound essential oil microemulsion and preparation method thereof |
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