CN105079118A - Natural tea tree oil agent used for improving human immunity and production process thereof - Google Patents
Natural tea tree oil agent used for improving human immunity and production process thereof Download PDFInfo
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Abstract
The invention relates to a natural tea tree oil agent used for improving human immunity and a production process of the natural tea tree oil agent. The production process includes the following steps: (1), mixing yeast extract with TTO to obtain a tea tree oil activating enzyme; (2), mixing TTO with the tea tree oil activating enzyme, and mixing TTO and the tea tree oil activating enzyme with air at an atomizing nozzle in a nitrogen atmosphere to be sprayed out, wherein atomized liquid which is sprayed out continues to be sprayed out from the atomizing nozzle; (3), raising the temperature to 30-35 DEG C, and repeating the process of spraying out; (4), raising the temperature to 45-55 DEG C, and repeating the process of spraying out; (5), raising the temperature to 75-80 DEG C, and repeating the process of spraying out; (6), obtaining a tea tree oil substrate when 15 main ingredients, meeting requirements, in natural tea tree oil are detected by using a gas chromatograph-mass spectrometer; (7), mixing the tea tree oil activating enzyme with the tea tree oil substrate, and adding ascorbic acid and beta-carotene oil to obtain the natural tea tree oil agent. The natural tea tree oil agent is edible and can improve human immunity when being used as an additive of human healthcare products.
Description
Technical field
The present invention relates to a kind of natural tea tree oil preparation and production technology thereof, especially a kind of natural tea tree oil preparation for improving human immunity power and production technology thereof.
Background technology
Australia natural tea tree oil (TeaTreeOil) is called for short TTO, the formal name used at school MelaleucaAlternifolia of tree.Australia is extracted quintessence oil in World War II, and for antiinflammatory, sterilization, the nineties scientist finds that the terpineol-4 in TTO has extremely strong antioxidation and Scavenging ability, makes have many scientists to start to be devoted to the research of this respect now.But at present, the natural TTO extracted can only external, generally can not eat.
The basic point daughter element of TTO is determined by original producton location raw material.Same seeds due to biology may be originated Different sources, and cause some trace molecule elements of TTO different.TTO has 100 various trace elements, and with the tea tree oil touchstone of international ISO4730 and Australia AS2782, be with 15 kinds of essential elements to determine quality control standards (QCS), these 15 kinds of Main Ingredients and Appearances are as shown in table 1.
Table 1
Summary of the invention
The object of the invention is to overcome the deficiencies in the prior art, a kind of natural tea tree oil preparation for improving human immunity power is provided, edible, and may be used for personal care product's additive, improve body immunity.
The present invention also provides a kind of natural tea tree oil preparation for improving human immunity power and production technology thereof, adopts this production technology, and no matter the tea tree oil plant growing place of production is different, all exists by 15 Main Ingredients and Appearances after the standard detection of ISO4730 and Australia AS2782.
According to technical scheme provided by the invention, the production technology of the described natural tea tree oil preparation for improving human immunity power, is characterized in that, comprise the following steps:
(1), after yeast extract and TTO being mixed by 6 ~ 8:1000, stir 3 ~ 5 hours in 35 ~ 40 DEG C in homogenizer, obtain tea tree oil kinase;
(2) multistage reactor tail end venting mouth often leaves, and passes into the nitrogen that concentration is 9999.9%, and nitrogen flow is 10 ~ 20ml/ minute; In multistage reactor, inject the tea tree oil kinase of TTO and 5% ~ 8% volumetric ratio, obtain mixing material; This mixing material and air mix at atomizer place and spray, and mixing material is 1 ~ 1.5:500 with the liquid air space ratio of air, and ejection pressure is 0.7 ~ 1MPa/cm
2, the atomized liquid after ejection proceeds to after atomizer place mixes with air and sprays, and injecting time is 2 ~ 4 hours; The aerosolized liquid particles particle diameter of described atomizer ejection is 50 ~ 90nm;
(3) multistage reactor is warming up to 30 ~ 35 DEG C, sprayed by atomizer after the mixing material continuation after step (2) process mixes with air, ejection pressure is 0.6 ~ 0.8MPa/cm
2, keep 1 ~ 2 hour;
(4) multistage reactor is warming up to 45 ~ 55 DEG C, sprayed by atomizer after the mixing material continuation after step (2) process mixes with air, ejection pressure is 0.6 ~ 0.8MPa/cm
2, keep 1 ~ 2 hour;
(5) multistage reactor is warming up to 75 ~ 80 DEG C, sprayed by atomizer after the mixing material continuation after step (2) process mixes with air, ejection pressure is 0.7 ~ 1MPa/cm
2, keep 2 ~ 4 hours;
(6) gas chromatograph-mass spectrum interlock instrument is used to detect, cooling is shut down when satisfactory 15 kinds of Main Ingredients and Appearances in natural tea tree oil being detected, if do not meet, detect again after repeating step (5), until cool after meeting the demands in taking out lower than when 30 DEG C, obtain tea tree oil substrate;
(7) by volumetric ratio 5 ~ 10:1000, tea tree oil kinase is mixed with tea tree oil substrate, add the ascorbic acid of mixture volumetric ratio 2% ~ 3% and the beta-carotene oil of 2% ~ 3% again, in 35 ~ 40 DEG C of mix and blends 5 ~ 7 hours in homogenizer, obtain described natural tea tree oil preparation;
(8) the natural tea tree oil preparation that step (7) obtains is put into rustless steel container, and it is stand-by to be sealed in room temperature preservation after nitrogen injection.
Further, the mixing speed of described homogenizer is 8000 ~ 10000 revs/min.
The present invention also provides a kind of natural tea tree oil preparation for improving human immunity power adopting above-mentioned production technology to obtain.
The described natural tea tree oil preparation for improving human immunity power, Main Ingredients and Appearance comprises: australene: 0.3 ~ 2%, sabinene: 0.03 ~ 1%, α-terpinene: 0.2 ~ 1%, limonene: 0.06 ~ 2%, paracymene: 0.5 ~ 4%, 1, 8-cineole: 0.8 ~ 5%, γ-terpinene: 3 ~ 12%, terpinolene: 0.7 ~ 2%, 4-terpineol: 58 ~ 69%, alpha-terpineol: 0.8 ~ 2%, aromadendrene: 0.3 ~ 1.2%, ledene: 0.3 ~ 5%, δ-cadinene: 0.5 ~ 4.9%, blue press alcohol: 0.1 ~ 2% and LVHUA 100,000 layers of alcohol: 0.3 ~ 1%.
The present invention has the following advantages:
(1) natural tea tree oil preparation of the present invention is the extractive matter obtained by special installation and technique, is green ecological environment-friendly products, except edible, can also be used for personal care product's additive, to improve body immunity.
(2) natural tea tree oil additive application of the present invention greatly can improve body immunity in personal care product.
(3) produce the natural tea tree oil preparation obtained according to production technology of the present invention, no matter its raw material tea tree oil growth place of production is different, and after the standard detection by ISO4730 and Australia AS2782, its 15 kinds of Main Ingredients and Appearances at least ten three kinds listed should exist.
Accompanying drawing explanation
Fig. 1 is the testing result figure of Scavenging ability in embodiment four, and in figure, cylinder from left to right represents carotin, fish oil, coenzyme Q10, astaxanthin, lycopene, deep-sea krill oil and natural tea tree oil preparation respectively.
Fig. 2 is the CD4 immunocyte comparison diagram of two groups of white mice in embodiment five.
Fig. 3 is the CD8 immunocyte comparison diagram of two groups of white mice in embodiment five.
Fig. 4 is the CD80 immunocyte comparison diagram of two groups of white mice in embodiment five.
Fig. 5 is the CD11b immunocyte comparison diagram of two groups of white mice in embodiment five.
In Fig. 2 ~ Fig. 5, black cylinder is first group of white mice, and white cylinder is second group of white mice.
Fig. 6 is that in embodiment six, white mice takes CD4 immunocyte comparison diagram before and after natural tea tree oil preparation.
Detailed description of the invention
Below in conjunction with specific embodiment, the invention will be further described.
Its principle of production technology of the present invention take nanotechnology as this, molecule haze after the atomization of liquid is placed in specific temperature province and carries out exchange reaction, in the fierce collision process of molecule in atomization, isomerism is there is in specified temp region, more thus the concentration contained by molecule adds, concentration contained by other molecule decreases, the unwanted material gases of part under a certain pressure, discharge reactor by the outlet of the presetting height of reactor, finally obtain described natural tea tree oil preparation.
Yeast extract used in the present invention is commercially available prod, and the Main Ingredients and Appearance of this yeast extract is polypeptide, aminoacid, flavour nucleotide, vitamin B group and trace element; Yeast extract and TTO prepare the composition shown in tea tree oil kinase his-and-hers watches 1 obtained and play activation, to dropping on outside composition shown in table 1, then do not play activation.In up to a hundred the repetition tests through nearly more than 10,000 chickens and hundreds of pigs, except tea tree oil kinase of the present invention, also do not find that other can the kinase of effective composition shown in activation table 1, the composition shown in tea tree oil kinase and table 1 has high specificity and high efficiency.
Embodiment one: a kind of production technology of the natural tea tree oil preparation for improving human immunity power, comprises the following steps:
(1), after yeast extract and TTO being pressed 6:1000 mixing, stir 3 hours in 35 DEG C in homogenizer, obtain tea tree oil kinase; The mixing speed of described homogenizer is 10000 revs/min;
(2) multistage reactor tail end venting mouth often leaves, and passes into the nitrogen that concentration is 9999.9%, and nitrogen flow is 10ml/ minute; In multistage reactor, inject the tea tree oil kinase of TTO and 5% volumetric ratio, obtain mixing material; This mixing material and air mix at atomizer place and spray, and the liquid air space of mixing material and air is than being 1:500, and ejection pressure is 0.7MPa/cm
2, the atomized liquid after ejection proceeds to after atomizer place mixes with air and sprays, and injecting time is 4 hours; The aerosolized liquid particles particle diameter of described atomizer ejection is 50nm;
(3) multistage reactor is warming up to 30 DEG C, sprayed by atomizer after the mixing material continuation after step (2) process mixes with air, ejection pressure is 0.6MPa/cm
2, keep 2 hours;
(4) multistage reactor is warming up to 45 DEG C, sprayed by atomizer after the mixing material continuation after step (2) process mixes with air, ejection pressure is 0.6MPa/cm
2, keep 2 hours;
(5) multistage reactor is warming up to 75 DEG C, sprayed by atomizer after the mixing material continuation after step (2) process mixes with air, ejection pressure is 0.7MPa/cm
2, keep 4 hours;
(6) gas chromatograph-mass spectrum interlock instrument is used to detect, cooling is shut down when satisfactory 15 kinds of Main Ingredients and Appearances in natural tea tree oil being detected, if do not meet, detect again after repeating step (5), until cool after meeting the demands in taking out lower than when 30 DEG C, obtain tea tree oil substrate;
(7) by volumetric ratio 5:1000, tea tree oil kinase is mixed with tea tree oil substrate, add the ascorbic acid of mixture volumetric ratio 2% and the beta-carotene oil of 2% again, in 35 DEG C of mix and blends 7 hours in homogenizer, obtain described natural tea tree oil preparation; The mixing speed of described homogenizer is 10000 revs/min;
(8) the natural tea tree oil preparation that step (7) obtains is put into rustless steel container, and it is stand-by to be sealed in room temperature preservation after nitrogen injection.
Embodiment two: a kind of production technology of the natural tea tree oil preparation for improving human immunity power, comprises the following steps:
(1), after yeast extract and TTO being pressed 8:1000 mixing, stir 5 hours in 40 DEG C in homogenizer, obtain tea tree oil kinase; The mixing speed of described homogenizer is 8000 revs/min;
(2) multistage reactor tail end venting mouth often leaves, and passes into the nitrogen that concentration is 9999.9%, and nitrogen flow is 20ml/ minute; In multistage reactor, inject the tea tree oil kinase of TTO and 8% volumetric ratio, obtain mixing material; This mixing material and air mix at atomizer place and spray, and the liquid air space of mixing material and air is than being 1.5:500, and ejection pressure is 1MPa/cm
2, the atomized liquid after ejection proceeds to after atomizer place mixes with air and sprays, and injecting time is 2 hours; The aerosolized liquid particles particle diameter of described atomizer ejection is 90nm;
(3) multistage reactor is warming up to 35 DEG C, sprayed by atomizer after the mixing material continuation after step (2) process mixes with air, ejection pressure is 0.8MPa/cm
2, keep 1 hour;
(4) multistage reactor is warming up to 55 DEG C, sprayed by atomizer after the mixing material continuation after step (2) process mixes with air, ejection pressure is 0.8MPa/cm
2, keep 1 hour;
(5) multistage reactor is warming up to 80 DEG C, sprayed by atomizer after the mixing material continuation after step (2) process mixes with air, ejection pressure is 1MPa/cm
2, keep 2 hours;
(6) gas chromatograph-mass spectrum interlock instrument is used to detect, cooling is shut down when satisfactory 15 kinds of Main Ingredients and Appearances in natural tea tree oil being detected, if do not meet, detect again after repeating step (5), until cool after meeting the demands in taking out lower than when 30 DEG C, obtain tea tree oil substrate;
(7) by volumetric ratio 10:1000, tea tree oil kinase is mixed with tea tree oil substrate, add the ascorbic acid of mixture volumetric ratio 3% and the beta-carotene oil of 3% again, in 40 DEG C of mix and blends 5 hours in homogenizer, obtain described natural tea tree oil preparation; The mixing speed of described homogenizer is 8000 revs/min;
(8) the natural tea tree oil preparation that step (7) obtains is put into rustless steel container, and it is stand-by to be sealed in room temperature preservation after nitrogen injection.
Embodiment three: a kind of production technology of the natural tea tree oil preparation for improving human immunity power, comprises the following steps:
(1), after yeast extract and TTO being pressed 7:1000 mixing, stir 4 hours in 36 DEG C in homogenizer, obtain tea tree oil kinase; The mixing speed of described homogenizer is 9000 revs/min;
(2) multistage reactor tail end venting mouth often leaves, and passes into the nitrogen that concentration is 9999.9%, and nitrogen flow is 15ml/ minute; In multistage reactor, inject the tea tree oil kinase of TTO and 6% volumetric ratio, obtain mixing material; This mixing material and air mix at atomizer place and spray, and the liquid air space of mixing material and air is than being 1.2:500, and ejection pressure is 0.8MPa/cm
2, the atomized liquid after ejection proceeds to after atomizer place mixes with air and sprays, and injecting time is 3 hours; The aerosolized liquid particles particle diameter of described atomizer ejection is 60nm;
(3) multistage reactor is warming up to 32 DEG C, sprayed by atomizer after the mixing material continuation after step (2) process mixes with air, ejection pressure is 0.7MPa/cm
2, keep 1.5 hours;
(4) multistage reactor is warming up to 50 DEG C, sprayed by atomizer after the mixing material continuation after step (2) process mixes with air, ejection pressure is 0.7MPa/cm
2, keep 1.5 hours;
(5) multistage reactor is warming up to 76 DEG C, sprayed by atomizer after the mixing material continuation after step (2) process mixes with air, ejection pressure is 0.8MPa/cm
2, keep 3 hours;
(6) gas chromatograph-mass spectrum interlock instrument is used to detect, cooling is shut down when satisfactory 15 kinds of Main Ingredients and Appearances in natural tea tree oil being detected, if do not meet, detect again after repeating step (5), until cool after meeting the demands in taking out lower than when 30 DEG C, obtain tea tree oil substrate;
(7) by volumetric ratio 8:1000, tea tree oil kinase is mixed with tea tree oil substrate, add the ascorbic acid of mixture volumetric ratio 2.5% and the beta-carotene oil of 2.5% again, in 36 DEG C of mix and blends 6 hours in homogenizer, obtain described natural tea tree oil preparation; The mixing speed of described homogenizer is 9000 revs/min;
(8) the natural tea tree oil preparation that step (7) obtains is put into rustless steel container, and it is stand-by to be sealed in room temperature preservation after nitrogen injection.
Embodiment four: the application of the external Scavenging ability of described natural tea tree oil preparation
Adopt carotin, fish oil, coenzyme Q10, astaxanthin, lycopene, deep-sea krill oil and natural tea tree oil preparation of the present invention to test the ability of respective scavenging free radicals respectively, under the condition of same time same concentrations, testing result as shown in Figure 1.As seen from Figure 1, natural tea tree oil preparation Scavenging ability of the present invention is four times of the deep-sea krill oil that health product are best in the market.
Embodiment five:
Observe two groups of healthy mices, the mixture of first group of healthy mice oral 20 μ l natural tea tree oil preparation every day and 50 μ l sunflower oils, serve on 5 days; Second group of healthy mice takes 50 μ l sunflower oils every day, serve on 5 days.After 5 days, two groups of white mice inspections change of serum C D4, CD8, CD80, CD11b immunocytes, testing result is as shown in Fig. 2 ~ Fig. 5.From Fig. 2 ~ Fig. 5, after first group of white mice takes natural tea tree oil preparation, CD4 and CD8 level is increased, thus improves the immunologic function of white mice.
Embodiment six:
Inspection 20 healthy mices change of serum C D4 immunocyte meansigma methods, then every day oral 20ul natural tea tree oil preparation, serve on 18 days.18 days afterwards check change of serum C D4 immunocyte and oral before carrying out contrast, result is as shown in Figure 6.In Fig. 6, often organize the CD4 immunocyte meansigma methods that block diagram represents every day, be followed successively by the CD4 immunocyte meansigma methods of the 1st day to the 18th day from left to right, the cylinder often organizing left side in block diagram represents the CD4 immunocyte meansigma methods before taking, and the cylinder on right side represents the CD4 immunocyte meansigma methods after taking.As shown in Figure 6, natural tea tree oil preparation adds the level of white mice CD4, thus improves the immunologic function of mice.
Embodiment seven:
Get and went out shell Sanhuang chicken 780 same day, random point 6 groups, adopt natural tea tree oil preparation to feed 45 days, in detail in table 2.
Group 1a in table 2,1b, 2a, 2b be by the natural tea tree oil dilution agent of different ppm concentration in the drinking water of chicken, wherein, in group 1a, 1b, 2a, 2b, the concentration of natural tea tree oil preparation is respectively 100ppm, 200ppm, 400ppm, 800ppm, No. 3, group is antibiotic group, No. 4, group is matched group, normal feedstuff is same feed formula, and antibiotic group whole process antibiotic and various vaccine, without growth hormone.No. 4 matched groups only use vaccine, without antibiotic and growth hormone.Finally statistics group 1a, 1b, 2a, 2b mortality rate are zero, and group 3 mortality rate is 3%, and group steps up 4 mortality rates 6%.
Table 2
Embodiment eight:
When 45 days, by group 2a, 2b in embodiment four, 3,4 respectively getting 10 carries out serum test at random, result is as shown in table 3.
Show 3:45 days Sanhuang chicken experimental chicken serum sample testing results
As can be seen from the serology results of table 3, group 2a (the interpolation concentration of natural tea tree oil is 400ppm) improves the nonspecific immunity function of chicken greatly.
From above-described embodiment four ~ embodiment eight, it is high that free radical ability removed by natural tea tree oil preparation, can be used for human body guarantor and build product, improve immunity.
Claims (4)
1., for improving a production technology for the natural tea tree oil preparation of human immunity power, it is characterized in that, comprise the following steps:
(1), after yeast extract and TTO being mixed by 6 ~ 8:1000, stir 3 ~ 5 hours in 35 ~ 40 DEG C in homogenizer, obtain tea tree oil kinase;
(2) multistage reactor tail end venting mouth often leaves, and passes into the nitrogen that concentration is 9999.9%, and nitrogen flow is 10 ~ 20ml/ minute; In multistage reactor, inject the tea tree oil kinase of TTO and 5% ~ 8% volumetric ratio, obtain mixing material; This mixing material and air mix at atomizer place and spray, and mixing material is 1 ~ 1.5:500 with the liquid air space ratio of air, and ejection pressure is 0.7 ~ 1MPa/cm
2, the atomized liquid after ejection proceeds to after atomizer place mixes with air and sprays, and injecting time is 2 ~ 4 hours; The aerosolized liquid particles particle diameter of described atomizer ejection is 50 ~ 90nm;
(3) multistage reactor is warming up to 30 ~ 35 DEG C, sprayed by atomizer after the mixing material continuation after step (2) process mixes with air, ejection pressure is 0.6 ~ 0.8MPa/cm
2, keep 1 ~ 2 hour;
(4) multistage reactor is warming up to 45 ~ 55 DEG C, sprayed by atomizer after the mixing material continuation after step (2) process mixes with air, ejection pressure is 0.6 ~ 0.8MPa/cm
2, keep 1 ~ 2 hour;
(5) multistage reactor is warming up to 75 ~ 80 DEG C, sprayed by atomizer after the mixing material continuation after step (2) process mixes with air, ejection pressure is 0.7 ~ 1MPa/cm
2, keep 2 ~ 4 hours;
(6) gas chromatograph-mass spectrum interlock instrument is used to detect, cooling is shut down when satisfactory 15 kinds of Main Ingredients and Appearances in natural tea tree oil being detected, if do not meet, detect again after repeating step (5), until cool after meeting the demands in taking out lower than when 30 DEG C, obtain tea tree oil substrate;
(7) by volumetric ratio 5 ~ 10:1000, tea tree oil kinase is mixed with tea tree oil substrate, add the ascorbic acid of mixture volumetric ratio 2% ~ 3% and the beta-carotene oil of 2% ~ 3% again, in 35 ~ 40 DEG C of mix and blends 5 ~ 7 hours in homogenizer, obtain described natural tea tree oil preparation;
(8) the natural tea tree oil preparation that step (7) obtains is put into rustless steel container, and it is stand-by to be sealed in room temperature preservation after nitrogen injection.
2. as claimed in claim 1 for improving the production technology of the natural tea tree oil preparation of human immunity power, it is characterized in that: the mixing speed of described homogenizer is 8000 ~ 10000 revs/min.
3. the natural tea tree oil preparation for improving human immunity power adopting the production technology described in claim 1 or 2 to obtain.
4. as claimed in claim 3 for improving the natural tea tree oil preparation of human immunity power, it is characterized in that: Main Ingredients and Appearance comprises: australene: 0.3 ~ 2%, sabinene: 0.03 ~ 1%, α-terpinene: 0.2 ~ 1%, limonene: 0.06 ~ 2%, paracymene: 0.5 ~ 4%, 1, 8-cineole: 0.8 ~ 5%, γ-terpinene: 3 ~ 12%, terpinolene: 0.7 ~ 2%, 4-terpineol: 58 ~ 69%, alpha-terpineol: 0.8 ~ 2%, aromadendrene: 0.3 ~ 1.2%, ledene: 0.3 ~ 5%, δ-cadinene: 0.5 ~ 4.9%, blue press alcohol: 0.1 ~ 2% and LVHUA 100,000 layers of alcohol: 0.3 ~ 1%.
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| CN106924441A (en) * | 2017-01-20 | 2017-07-07 | 刘玥 | Return the application of Chinese herbaceous peony tea |
| CN111991311A (en) * | 2020-08-29 | 2020-11-27 | 荣鼎(广东)生物科技有限公司 | Production process of olive oil-containing nano essence |
| CN112089672A (en) * | 2020-08-26 | 2020-12-18 | 荣鼎(广东)生物科技有限公司 | Nano essence containing tea tree oil |
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| CN106924441A (en) * | 2017-01-20 | 2017-07-07 | 刘玥 | Return the application of Chinese herbaceous peony tea |
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