CN105104048B - It is a kind of to induce identification, the method for screening rice anti-rice blast breeding material - Google Patents
It is a kind of to induce identification, the method for screening rice anti-rice blast breeding material Download PDFInfo
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- CN105104048B CN105104048B CN201510629436.1A CN201510629436A CN105104048B CN 105104048 B CN105104048 B CN 105104048B CN 201510629436 A CN201510629436 A CN 201510629436A CN 105104048 B CN105104048 B CN 105104048B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/20—Cereals
- A01G22/22—Rice
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
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Abstract
The invention discloses a kind of methods for inducing identification, screening rice anti-rice blast breeding material, use for reference green house of vegetables facility, build pergola facility, increase spray and sunshade net, the optimal onset environment of manual simulation, ensure the generation of rice blast, by cultivating bacterium solution, manual simulation's high humidity environment, induce the big generation of rice blast, rice blast mixed bacteria liquid is inoculated with by boot stage spray-on process, expand rice blast microspecies radix, it is ensured that bacterium source and a variety of biological strains, so as to screen, create anti-rice blast rice breed；The present invention has the advantages of labor intensity is low, and anti-pest screening efficiency is high, the equal blast resisting of elected breeding material, reduces the blindness of breeding, improves the efficiency of selection of anti-rice blast, and easy to operate, dart transfer.
Description
Technical field
The present invention relates to the breeding technical fields of rice more particularly to a kind of induction identification, screening rice anti-rice blast to educate
The method of kind material.
Background technology
Rice blast is one of hazard rice most serious disease, and the grain loss as caused by rice blast accounts for whole world grain
The 11%~30% of food loss, the serious good quality and high output for influencing China's rice, and the research on utilization of variety resistance is then public
It is considered the basic strategy of comprehensive control of disease.Many breeders want selection and breeding high resistant to rice blast product by various methods
Kind, but all produce little effect, main reason is that blast resisting evaluation and screening difficulty is big, work efficiency is low.In current anti-rice
In the large-scale inducing of seasonal febrile diseases material, identification, screening process, rice blast fungus cannot fully be met to high humidity by existing
Requirement, and the defects of it is difficult that Pyricularia oryzae is caused to be sprouted, and artificial infection induces small scale, heavy workload.
A kind of new technical solution is needed to solve problem above.
The content of the invention
Goal of the invention：In order to overcome the rice blast fungus in the prior art that cannot fully meet to high humidity
Requirement, and the problem of it is difficult that Pyricularia oryzae is caused to be sprouted, and artificial infection induces small scale, heavy workload, the present invention proposes
A kind of labor intensity is low, the high induction identification of anti-pest screening efficiency, the method for screening rice anti-rice blast breeding material.
Technical solution：In order to solve the above-mentioned technical problem, the technical solution adopted in the present invention is：A kind of induction identification,
The method for screening rice anti-rice blast breeding material, comprises the following steps：
(1) the conidial acquisition of rice blast fungus：Prior year is local representative in acquisition across the entire province
Rice blast bacterial strain；
(2) frame spray Facilities Construction：Steelframe beam-connected greenhouse is built, ceiling is 2.5 meters high, 2 meters of level height, every 1.8 meters
A thicker wire is led, the spray head that spray radius is 1 meter is hung for every 1.8 meters on steel wire；Ceiling covers the sunshade net of 70 mesh, can receive
It puts freely, usually packs up spare；
(3) proper seeding：Late May, by rice breed by the greenhouse facility that is set in step (2) of seed selection requirement
It is sowed, induces rice varieties and sow 1 part at interval of 20 materials with reference to seed selection material, do not prevent curing the disease in entire breeding time
Evil；Pest control and rich water are used with reference to Production of Large Fields；
(4) preparation of rice blast mixed bacteria liquid：The rice blast bacterial strain that step (1) gathers is implanted in RCA culture mediums
On, cultivated 7 days at 25 DEG C, with black light light irradiation 72 it is small when after, after Pyricularia oryzae generates spore, then with sterile water wash,
It is made under 10 × 10 power microscopes per the suspension of 30-40, visual field spore, refrigeration is spare；
(5) boot stage uses the rice blast mixed bacteria liquid that spray-on process inoculation step (4) is prepared, and expands rice blast microspecies base
Number；
(6) in rice pulvinus usually, spraying rice blast mixed bacteria liquid is inoculated at dusk, puts down within the 2nd day sunshade net, and every 1
Hour spray 10 minutes, continuous 15 days；
(7) observe, record and statistics, final election：Observe the generation of rice blast disease in entire breeding time, especially water
Rice leaf pulvinus is to the maturity period, by the incidence of unification of the motherland standard survey Rice blast；Investigate false smut disease fruit grain simultaneously；
Elected material of the rice blast disease tassel yield less than 10.0% or leaf pest are less than 2 grades, material of the disease tassel yield less than 15.0%；
(8) material of the good blast resisting of prior year selection and breeding is repeated above step by second year in greenhouse facility again,
Confirm that the intermediate materials of neat blast resisting are stablized in selection and breeding.
Further, rice blast bacterial strain representative in the step (1) is 014-290-1 in Jiangsu Province
(ZB27)、2014-30-1(ZC15)、2014-56-2(ZD7)、2010-55-1(ZE3)、2014-284-2(ZF1)、2014-
303-1 (ZG1) amounts to the bacterium of 6 microspecies.
Further, seed selection requirement is that general low generation material application rate is 60 grams in the step (3), area 4
Square metre；Advanced lines material application rate be 200, specification be 1.5 meters of drillings, 30 centimetres of line-spacing.
Further, it is susceptible variety Suyunuo that rice varieties are induced in the step (3).
Further, the specific culture medium prescriptions of RCA are 40 grams of corn flour, 50 grams of rice straw, agar 20 in the step (4)
Gram.
Advantageous effect：A kind of method for inducing identification, screening rice anti-rice blast breeding material provided by the invention, is used for reference
Green house of vegetables facility builds pergola facility, increases spray and sunshade net, the optimal onset environment of manual simulation, it is ensured that rice blast
Occur, by cultivating bacterium solution, manual simulation's high humidity environment, induce the big generation of rice blast, rice blast is inoculated with by boot stage spray-on process
Sick mixed bacteria liquid expands rice blast microspecies radix, it is ensured that bacterium source and a variety of biological strains, so as to screen, create blast resisting water
Rice breeding material；The present invention has the advantages of labor intensity is low, and anti-pest screening efficiency is high, the anti-rice blast of elected breeding material
Disease reduces the blindness of breeding, improves the efficiency of selection of anti-rice blast, and easy to operate, dart transfer.
Description of the drawings
Fig. 1 is the breeding procedures schematic diagram of the blast resisting material of one embodiment of the present invention.
Specific embodiment
With reference to embodiment, the present invention is described in further detail：
It, seldom should in large area production since comprehensive yielding ability is not high though anti-rice blast resource has stronger resistance
With this just needs breeder using anti-rice blast resource to improve current main breed；Combos a large amount of first screen strong advantage group
It closes.
Fig. 1 is refer to, it is a kind of to induce identification, the method for screening rice anti-rice blast breeding material, comprise the following steps：
(1) the conidial acquisition of rice blast fungus：Prior year is gathering representative rice across the entire province
Rice blast bacterial strain；It is 014-290-1 (ZB27) in Jiangsu Province, 2014-30-1 (ZC15), 2014-56-2 (ZD7), 2010-55-1
(ZE3), 2014-284-2 (ZF1), 2014-303-1 (ZG1) amount to the bacterium of 6 microspecies；
(2) frame spray Facilities Construction：Steelframe beam-connected greenhouse is built, ceiling is 2.5 meters high, 2 meters of level height, every 1.8 meters
A thicker wire is led, the spray head that spray radius is 1 meter is hung for every 1.8 meters on steel wire；Ceiling covers the sunshade net of 70 mesh, can receive
It puts freely, usually packs up spare；
(3) proper seeding：Late May, by rice breed by the greenhouse facility that is set in step (2) of seed selection requirement
It is sowed, seed selection requirement is specially that general low generation material application rate is 60 grams, and area is 4 square metres；Advanced lines material is broadcast
Kind of amount is 200, and specification is 1.5 meters of drillings, 30 centimetres of line-spacing；Rice varieties, that is, susceptible variety Suyunuo is induced with reference to seed selection material
Material sows 1 part at interval of 20 materials, not controlling disease in entire breeding time；Pest control and rich water are used gives birth to reference to crop field
Production；
(the preparation of (4) rice blast mixed bacteria liquid：The rice blast bacterial strain that step (1) gathers is implanted in RCA culture mediums
On, the specific culture medium prescriptions of RCA are 40 grams of corn flour, 50 grams of rice straw, 20 grams of agar；It cultivates 7 days at 25 DEG C, is shone with black light lamp
Penetrate 72 it is small when after, after Pyricularia oryzae generates spore, then with sterile water wash, be made under 10 × 10 power microscopes per visual field 30-
The suspension of 40 spores, refrigeration are spare；
(5) boot stage uses the rice blast mixed bacteria liquid that spray-on process inoculation step (4) is prepared, and expands rice blast microspecies base
Number；
(6) in rice pulvinus usually, spraying rice blast mixed bacteria liquid is inoculated at dusk, puts down within the 2nd day sunshade net, and every 1
Hour spray 10 minutes, continuous 15 days；
(7) observe, record and statistics, final election：Observe the generation of rice blast disease in entire breeding time, especially water
Rice leaf pulvinus is to the maturity period, by the incidence of unification of the motherland standard survey Rice blast；Investigate false smut disease fruit grain simultaneously；
Elected material of the rice blast disease tassel yield less than 10.0% or leaf pest are less than 2 grades, material of the disease tassel yield less than 15.0%；
(8) material of the good blast resisting of prior year selection and breeding is repeated above step by second year in greenhouse facility again,
Confirm that the intermediate materials of neat blast resisting are stablized in selection and breeding.
Wherein, unification of the motherland leaf blast in rice grade scale is as follows：
0 grade：It is disease-free.
1 grade：The only brown dot of needle point size.
2 grades：There is larger brown point.
3 grades：Circle to ellipse grey scab, edge brown, 1~2cm of diameter.
4 grades：Typical spindle scab, long 1~2cm, is generally confined between two veins, and area of causing harm is less than leaf area
2%.
5 grades：Typical spindle scab, area of causing harm account for the 2~10% of leaf area.
6 grades：Typical spindle scab, area of causing harm account for the 11~25% of leaf area.
7 grades：Typical spindle scab, area of causing harm account for the 26~50% of leaf area.
8 grades：Typical spindle scab, area of causing harm account for the 51~75% of leaf area.
9 grades：Typical spindle scab, area of causing harm are more than the 75% of leaf area
Rice blast grade scale
Rice blast incidence population resistance grade scale is as follows：
Fringe pest incidence (the %)=total spike number * 100 of disease spike number/investigation
Rice blast loss index population resistance grade scale is as follows：
Loss index (%)=∑ (morbidity number * at different levels loss lates at different levels)/(investigate the highest damage of total spike number * grade scales
Mistake rate) * 100
Rice blast resistance composite index Assessment for classification standard is as follows：
Composite index=(leaf seasonal febrile diseases grade * 25%+ fringe pest incidence disease grade * 25%+ fringe pest loss index disease grades * 50%)
A kind of method for inducing identification, screening rice anti-rice blast breeding material provided by the invention, uses for reference green house of vegetables
Facility builds pergola facility, increases spray and sunshade net, the optimal onset environment of manual simulation, it is ensured that the generation of rice blast passes through
Bacterium solution, manual simulation's high humidity environment are cultivated, induces the big generation of rice blast, rice blast Mixed Microbes are inoculated with by boot stage spray-on process
Liquid expands rice blast microspecies radix, it is ensured that bacterium source and a variety of biological strains, so as to screen, create anti-rice blast rice breeding material
Material；The present invention has the advantages of labor intensity is low, and anti-pest screening efficiency is high, and the equal blast resisting of elected breeding material reduces
The blindness of breeding improves the efficiency of selection of anti-rice blast, and easy to operate, dart transfer.
It should be pointed out that more than specific embodiment is only illustrative of the invention and is not intended to limit the scope of the invention,
After having read the present invention, those skilled in the art fall within the modification of the various equivalent forms of the present invention power appended by the application
Profit requires limited range.
Claims (1)
1. a kind of induce identification, the method for screening rice anti-rice blast breeding material, it is characterised in that comprises the following steps：
（1）The conidial acquisition of rice blast fungus：Prior year is in the local representative rice of acquisition across the entire province
Rice blast bacterial strain；The representative rice blast bacterial strain is 014-290-1 in Jiangsu Province（ZB27）、2014-30-1
（ZC15）、2014-56-2（ZD7）、2010-55-1（ZE3）、2014-284-2（ZF1）、2014-303-1（ZG1）, amount to 6
The bacterium of microspecies；
（2）Frame sprays Facilities Construction：Steelframe beam-connected greenhouse is built, ceiling is 2.5 meters high, 2 meters of level height, and every 1.8 meters are led one
Thicker wire, the spray head that every 1.8 meters of suspensions spray radius is 1 meter on steel wire；Ceiling covers the sunshade net of 70 mesh, and energy folding and unfolding is certainly
Such as, usually pack up spare；
（3）Proper seeding：Late May, by rice breed by seed selection requirement in step（2）It is carried out in the greenhouse facility of setting
Sowing induces rice varieties and sows 1 part at interval of 20 materials with reference to seed selection material, not controlling disease in entire breeding time；Worm
Evil prevention and rich water are used with reference to Production of Large Fields；Induce rice varieties specific choice susceptible variety Suyunuo；Seed selection requirement is low
Generation material application rate is 60 grams, and area is 4 square metres；Advanced lines material application rate is 200, and specification is 1.5 meters of drillings, is gone
Away from 30 centimetres；
（4）The preparation of rice blast mixed bacteria liquid：By step（1）The rice blast bacterial strain of acquisition is implanted on RCA culture mediums,
Cultivated 7 days at 25 DEG C, with black light light irradiation 72 it is small when after, after Pyricularia oryzae generates spore, then with sterile water wash, be made into 10
Per the suspension of 30-40, visual field spore under × 10 power microscopes, refrigeration is spare；The specific culture medium prescriptions of RCA are corn flour 40
Gram, 50 grams of rice straw, 20 grams of agar；
（5）Boot stage uses spray-on process inoculation step（4）The rice blast mixed bacteria liquid of preparation expands rice blast microspecies radix；
（6）In rice pulvinus usually, spraying rice blast mixed bacteria liquid is inoculated at dusk, puts down within the 2nd day sunshade net, it is every 1 it is small when
Spray 10 minutes, continuous 15 days；
（7）Observation is recorded and statistics, final election：The generation of rice blast disease in entire breeding time is observed, by unification of the motherland mark
The incidence of quasi- investigation Rice blast；Investigate false smut disease fruit grain simultaneously；Elected rice blast disease tassel yield is less than 10.0% material
Material or leaf pest are less than 2 grades, material of the disease tassel yield less than 15.0%；
（8）The material of the good blast resisting of prior year selection and breeding is repeated above step by second year in greenhouse facility again, is confirmed
The intermediate materials of neat blast resisting are stablized in selection and breeding.
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| CN105624264A (en) * | 2015-12-26 | 2016-06-01 | 黑龙江省农业科学院佳木斯水稻研究所 | Two-nursery-to-one stress resistance identification technology |
| CN106416654A (en) * | 2016-07-26 | 2017-02-22 | 福建省农业科学院植物保护研究所 | Corn southern leaf blight field natural inducement technology and application of technology |
| CN106544396B (en) * | 2016-10-18 | 2020-10-23 | 湖南杂交水稻研究中心 | Method for identifying indoor rice blast and rapidly breeding disease-resistant rice |
| CN106957890B (en) * | 2017-03-22 | 2021-02-26 | 恩施土家族苗族自治州农业科学院 | Method for improving indoor artificial inoculation identification accuracy of rice variety panicle blast |
| CN107593427B (en) * | 2017-09-28 | 2020-02-21 | 江苏丘陵地区镇江农业科学研究所 | Breeding method of rice with high-resistance and stable-rice-blast-resistance performance |
| CN110432098A (en) * | 2019-07-30 | 2019-11-12 | 江苏沿海地区农业科学研究所 | A kind of method of breeding rice blast resisting breeding intermediate materials |
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| CN1326671A (en) * | 2000-06-05 | 2001-12-19 | 华中农业大学 | Breeding method for anti-rice blast rice breed |
| CN100513554C (en) * | 2007-04-19 | 2009-07-15 | 江苏丘陵地区镇江农业科学研究所 | Short small bacillus TW and viable bacteria preparation for rice blast prevention and cure |
| CN103563991A (en) * | 2012-07-31 | 2014-02-12 | 江苏丘陵地区镇江农业科学研究所 | Preparation method of brevibacillus brevis water dispersible granules |
| CN103875524B (en) * | 2014-03-21 | 2016-08-17 | 江苏丘陵地区镇江农业科学研究所 | A kind of breeding method of high setting percentage Oryza sativa L. |
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