CN110432098A - A kind of method of breeding rice blast resisting breeding intermediate materials - Google Patents
A kind of method of breeding rice blast resisting breeding intermediate materials Download PDFInfo
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- CN110432098A CN110432098A CN201910724375.5A CN201910724375A CN110432098A CN 110432098 A CN110432098 A CN 110432098A CN 201910724375 A CN201910724375 A CN 201910724375A CN 110432098 A CN110432098 A CN 110432098A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
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Abstract
The present invention relates to a kind of methods of breeding rice blast resisting breeding intermediate materials, belong to Genetic and breeding in rice design field, it is characterized in that, it (1) can be in areal, carry out the screening of multiple and different rice blast biological strains, (2) anti-rice blast epilepsy gradient can be carried out to same material and selected in different regions.Make breeding intermediate materials in the case where a variety of physiological races of rice blast fungus select pressure, obtains the breeding material of wide spectrum highly resistance.Material to be tested is planted in different regions, local meteorological data is observed and recorded, filters out the rice breed that can adapt to different diseases pressure.The invention patent improves the efficiency of breeder's breeding rice blast resisting intermediate materials by the screening using different regions rice blast biological strain and different regions Disease pressure progress blast resisting, has the advantages that quick, easy, economical.
Description
Technical field
The present invention relates to a kind of methods of breeding rice blast resisting breeding intermediate materials, belong to Genetic and breeding in rice design
Technical field.
Background technique
Rice blast is one of important disease on the rice as caused by Pyricularia oryzae (Magnaporthe oryzae), right
Yield and quality of rice has important influence, the underproduction 40%~50% can be caused when serious, or even No kernels or seeds are gathered, as in a year of scarcity.Breeding and utilization
Disease-resistant variety is one of prevention and treatment most economical effective method of rice blast, but since there are multiple biological strains, antibacterials for Pyricularia oryzae
Composing relatively narrow kind, often disease resistance may reduce after promoting several years.Breeder is mainly connect by Pyricularia oryzae
Kind identification, resistant gene screening, the method progress disease resistance breeding of molecular biology.It can filter out to a certain extent disease-resistant
The material of property, but there are larger workloads, it is desirable that breeding units have the instrument and equipment of higher-end, the talent of high-tech and examination
It tests costly, is difficult to carry out for base's breeding units and breeding companies.For the problem present on, base's breeding list
Position and breeding companies need simple, the efficient technical method of one kind to solve rice blast resistance Breeding issue.
Summary of the invention
Goal of the invention: rice blast resistance material is largely screened in order to overcome the prior art not to be able to satisfy base's breeding units
Problem, and rice anti-rice blast bacterium breeding is caused to develop slowly；Low, expert evidence quantity that the invention proposes a kind of labor intensity
The method of the high rice anti-rice blast breeding intermediate materials of more, anti-pest screening efficiency.
Technical solution: in order to solve the above-mentioned technical problem, the technical scheme adopted by the invention is as follows: a kind of breeding rice is anti-
The method of rice blast breeding intermediate materials, comprising the following steps:
(1) acquisition rice blast is fallen ill diseased plant: the sick straw to carry disease germs is being acquired across the entire province, is placed on warehouse and is dried in the shade preservation,
Allow germ safe overwintering on sick straw, sprouts spore to second year and infected；
(2) selection can represent the plot of objective area rice pathogenesis degree, for the plantation of material to be tested, by different mesh
The morbidity garden in mark area constitutes identification net, and there are gradient disparities for the rice blast occurring degree of difference identification net；
(3) proper seeding: early June plants same group of material to be tested in each test morbidity garden according to test requirements document
In, wherein susceptible variety is a every 10 material sowings, and each two column broadcast a column, not anti-in entire growth period duration of rice to cure the disease
Evil, pest control and liquid manure are used referring to field management, are recorded in the paddy growth period, the state of an illness in each period and meteorological money
Material；
(4) rice blast conidium induction is sprouted: sick straw shredded with knife, is squirted the sick straw of crushing with watering can,
And moisture-heat preservation is covered with film；
(5) leaf pest are identified: rice plant of tillering stage, using the method manually shed, in the cloudy sick rice that will be crushed in (4) at dusk
Grass is uniformly spread to Tanaka, for three days on end；
(6) fringe pest is identified: Rise's boot period, using the method manually shed, in the cloudy sick rice that will be crushed in (4) at dusk
Grass is uniformly spread to Tanaka, for three days on end；
(7) it observes, count and chooses seeds: in tillering stage to rice maturity, observing field rice rice blast incidence, press
It is recorded according to national rice blast morbidity standard, chooses the good material of economical character；
(8) the breeding intermediate materials second year chosen is continued to plant, repeats above step, then the sick rice that field is fallen ill
Grass manually carries out harvesting preservation, the germ source of infection for second year.
Further, the rice blast morbidity diseased plant acquired in the step (1) is adopted on each objective area morbidity garden periphery
Sample, multidraw, the diseased plant fringe portion and panicle neck of selection are withered, or even spread to entire plant.
Further, selection can represent the plot of objective area rice pathogenesis degree in the step (2), and feature is in root
According to different year rice scientific data, the rice blast occurring degree of the morbidity garden point in each area is had differences, and can be sieved
Select the anti-rice blast rice breed for adapting to objective area.
Further, it is 50g, area 3m2, each material that seed selection requirement, which is material to be tested application rate, in the step (3)
Between line-spacing be 30cm.Corresponding meteorological observation equipment is installed on the point of each morbidity garden.
Further, it is susceptible variety OryzasativaLcv.Nipponbare that rice varieties are induced in the step (3).
Further, sick straw is cut into 5~8cm in the step (4), and humidity will reach 90% or more, and temperature will be maintained at
24~28 DEG C of ranges.
Further, the amount that disease straw is manually shed in the step (5) (6) is 120kg/hm2, for three days on end the dusk at cloudy day
Disease straw is shed, is conducive to raising Pyricularia oryzae and initially infects bacterium amount, improve the disease incidence of susceptible variety.
Further, the step (7) chooses the good material of economical character, the rice blast morbidity in the morbidity garden of different regions
Rate is low, and for plant height in 1m or so, spike number is more, and be in the milk the good material of full and yield traits.
The utility model has the advantages that the present invention provides a kind of this method of breeding rice blast resisting breeding intermediate materials, samely
Area carries out the screening of multiple and different regional rice blast biological strains, by interspersed kind of height sense rice blast material OryzasativaLcv.Nipponbare kind
It plants among rice is for examination breeding material, the Culm of Rice that then rice blast acquired from different regions is fallen ill, chopping is spread on
Field, induction is for examination rice material morbidity.According to the incidence for trying breeding material, different rice materials to be tested are resisted
Property classification, screening and identification go out can it is disease-resistant to different regions rice blast biological strain for try rice breeding intermediate materials, improve
Screening efficiency.In different target area, the selection of blast resisting breeding intermediate materials is carried out, can filter out and adapt to different regions
Anti-rice blast rice breed.The invention patent is by utilizing different regions rice blast biological strain and different regions disease
Pressure carries out the screening of blast resisting, makes breeder in breeding breeding intermediate materials, for trying the blast resisting energy of breeding material
Natural Selection has been obtained in power, it is only necessary to the economical character for trying breeding material is paid close attention to, to improve rice breeding intermediate wood
The screening efficiency of material has the advantages that quick, easy, economical.
Specific embodiment
Below with reference to embodiment, the present invention is described in further detail:
A kind of method of breeding rice blast resisting breeding intermediate materials, comprising the following steps:
(1) acquisition rice blast is fallen ill diseased plant: the sick straw to carry disease germs is being acquired across the entire province, is placed on warehouse and is dried in the shade preservation,
Allow germ safe overwintering on sick straw, sprouts spore to second year and infected；
(2) selection can represent the plot of objective area rice pathogenesis degree, for the plantation of material to be tested, by different mesh
The morbidity garden in mark area constitutes identification net, and there are gradient disparities for the rice blast occurring degree of difference identification net；
(3) proper seeding: early June plants same group of material to be tested in each test morbidity garden according to test requirements document
In, wherein susceptible variety is a every 10 material sowings, and each two column broadcast a column, not anti-in entire growth period duration of rice to cure the disease
Evil, pest control and liquid manure are used referring to field management, are recorded in the paddy growth period, the state of an illness in each period and meteorological money
Material；
(4) rice blast conidium induction is sprouted: sick straw shredded with knife, is squirted the sick straw of crushing with watering can,
And moisture-heat preservation is covered with film；
(5) leaf pest are identified: rice plant of tillering stage, using the method manually shed, in the cloudy sick rice that will be crushed in (4) at dusk
Grass is uniformly spread to Tanaka, for three days on end；
(6) fringe pest is identified: Rise's boot period, using the method manually shed, in the cloudy sick rice that will be crushed in (4) at dusk
Grass is uniformly spread to Tanaka, for three days on end；
(7) it observes, count and chooses seeds: in tillering stage to rice maturity, observing field rice rice blast incidence, press
It is recorded according to national rice blast morbidity standard, chooses the good material of economical character；
(8) the breeding intermediate materials second year chosen is continued to plant, repeats above step, then the sick rice that field is fallen ill
Grass manually carries out harvesting preservation, the germ source of infection for second year.
Claims (8)
1. a kind of identification method of breeding rice blast resisting breeding intermediate materials, feature include the following steps:
(1) acquisition rice blast is fallen ill diseased plant: being acquired the sick straw to carry disease germs across the entire province, is being placed on warehouse and dries in the shade preservation, make disease
Bacterium can on sick straw safe overwintering, to second year sprout spore infected；
(2) selection can represent the plot of objective area rice pathogenesis degree, for the plantation of material to be tested, by different target
The morbidity garden in area constitutes identification net, and there are gradient disparities for the rice blast occurring degree of difference identification net；
(3) proper seeding: early June plants same group of material to be tested in each test morbidity garden according to test requirements document,
Middle susceptible variety is a every the sowing of 10 materials, and each two column broadcast a column, not controlling disease in entire growth period duration of rice, insect pest
Prevention and treatment and liquid manure are used referring to field management, are recorded in the paddy growth period, the state of an illness and meteorological data in each period；
(4) rice blast conidium induction is sprouted: sick straw being shredded with knife, the sick straw of crushing is squirted with watering can, is used in combination
Film covers moisture-heat preservation；
(5) leaf pest are identified: rice plant of tillering stage, equal in the cloudy sick straw that will be crushed in (4) at dusk using the method manually shed
Even is spread to Tanaka, for three days on end；
(6) fringe pest is identified: Rise's boot period, equal in the cloudy sick straw that will be crushed in (4) at dusk using the method manually shed
Even is spread to Tanaka, for three days on end；
(7) it observes, count and chooses seeds: in tillering stage to rice maturity, field rice rice blast incidence is observed, according to state
Family's rice blast morbidity standard is recorded, and the good material of economical character is chosen；
(8) the breeding intermediate materials second year chosen is continued to plant, repeats above step, then the sick straw that field is fallen ill,
Harvesting preservation manually is carried out, the germ source of infection for second year.
2. a kind of method of breeding rice blast resisting breeding intermediate materials according to claim 1, it is characterised in that: institute
The rice blast morbidity diseased plant acquired in step (1) is stated, is sampled on each objective area morbidity garden periphery, multidraw, selection
Diseased plant fringe portion and panicle neck are withered, or even spread to entire plant.
3. a kind of method of breeding rice blast resisting breeding intermediate materials according to claim 1, it is characterised in that: institute
The plot of objective area rice pathogenesis degree can be represented by stating selection in step (2), and feature is according to different year rice section
Data are ground, the rice blast occurring degree of the morbidity garden point in each area has differences, and can filter out and adapt to objective area
Anti-rice blast rice breed.
4. a kind of method of breeding rice blast resisting breeding intermediate materials according to claim 1, it is characterised in that: institute
It states to choose seeds in step (3) and requires to be material to be tested application rate to be 50g, area 3m2, the line-spacing between each material is 30cm.
Corresponding meteorological observation equipment is installed on the point of each morbidity garden.
5. a kind of method of breeding rice blast resisting breeding intermediate materials according to claim 1, it is characterised in that: institute
It states and induces rice varieties in step (3) for susceptible variety OryzasativaLcv.Nipponbare.
6. a kind of method of breeding rice blast resisting breeding intermediate materials according to claim 1, it is characterised in that: institute
It states sick straw in step (4) and is cut into 5~8cm, humidity will reach 90% or more, and temperature will be maintained at 24~28 DEG C of ranges.
7. a kind of identification method of breeding rice blast resisting breeding intermediate materials according to claim 1, feature exist
In: the amount that disease straw is manually shed in the step (5) and (6) is 120kg/hm2, and the cloudy day sheds disease straw at dusk for three days on end,
Be conducive to raising Pyricularia oryzae and initially infect bacterium amount, improves the disease incidence of susceptible variety.
8. a kind of identification method of breeding rice blast resisting breeding intermediate materials according to claim 1, feature exist
In: the step (7) chooses the good material of economical character, and rice blast disease incidence is low in the morbidity garden of different regions, and plant height is in 1m
Left and right, spike number is more, and be in the milk the good material of full and yield traits.
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| CN201910724375.5A CN110432098A (en) | 2019-07-30 | 2019-07-30 | A kind of method of breeding rice blast resisting breeding intermediate materials |
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| CN201910724375.5A CN110432098A (en) | 2019-07-30 | 2019-07-30 | A kind of method of breeding rice blast resisting breeding intermediate materials |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114158441A (en) * | 2021-10-24 | 2022-03-11 | 江苏沿海地区农业科学研究所 | Breeding method of new rice variety of japonica rice with early maturity, fast dehydration and fast rice blast resistance |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104381125A (en) * | 2014-11-19 | 2015-03-04 | 长江大学 | Authentication method of rice blast disease resistant breeding material |
| CN105104048A (en) * | 2015-09-28 | 2015-12-02 | 江苏丘陵地区镇江农业科学研究所 | Method for inducing, identifying and screening rice blast resistance breeding materials |
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- 2019-07-30 CN CN201910724375.5A patent/CN110432098A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104381125A (en) * | 2014-11-19 | 2015-03-04 | 长江大学 | Authentication method of rice blast disease resistant breeding material |
| CN105104048A (en) * | 2015-09-28 | 2015-12-02 | 江苏丘陵地区镇江农业科学研究所 | Method for inducing, identifying and screening rice blast resistance breeding materials |
Non-Patent Citations (1)
| Title |
|---|
| 张玉华 等: "水稻稻瘟病活体菌株接种法在稻种资源抗性鉴定中的应用", 《黑龙江农业科学》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114158441A (en) * | 2021-10-24 | 2022-03-11 | 江苏沿海地区农业科学研究所 | Breeding method of new rice variety of japonica rice with early maturity, fast dehydration and fast rice blast resistance |
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