CN105087398A - Mixed inoculant agent for effective degradation of mushroom bran, and preparation method and application thereof - Google Patents
Mixed inoculant agent for effective degradation of mushroom bran, and preparation method and application thereof Download PDFInfo
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- CN105087398A CN105087398A CN201510569108.7A CN201510569108A CN105087398A CN 105087398 A CN105087398 A CN 105087398A CN 201510569108 A CN201510569108 A CN 201510569108A CN 105087398 A CN105087398 A CN 105087398A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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Abstract
The present invention relates to a mixed inoculant agent for effective degradation of mushroom bran. The mixed inoculant agent is prepared by mixing a straw fermentation broth with a crude fiber degrading broth by 1:5-5:1 to prepare a mixed inoculant stock solution, then inoculating 0.25%-1.0% of the mixed stock solution into sterile distilled water, adding a mixed broth containing Aspergillus niger and Penicillium and accounting for 1.0-5.0 wt.% of the sterile distilled water, and stably culturing for 2-3 d. The preparation method of the mixed broth is as follows: inoculating Aspergillus niger and Penicillium to a PDA slant medium for 3-4 d respectively, washing the spores on the slant with sterile water to prepare a spore suspension with viable cell count of 106 CFU/mL; and conducting liquid fermentation culture on the Aspergillus niger and Penicillium according to the proportion of 15%-25% for 7-8 d to obtain a mixed fermentation broth. The mixed inoculant agent of the present invention to is convenient for the mushroom bran degradation, and has obvious degradation effect.
Description
Technical field
The present invention relates to the degraded field of edible mushrooms trade waste, particularly relate to mix bacterium agent of a kind of effective degradation bacteria chaff and its preparation method and application.
Background technology
Bacterium chaff, is again bacterium slag, and the substratum waste produced after referring to culturing edible fungus, mainly comprises the cellulose components such as cotton seed hull, wood chip and stalk.In recent years, along with the continuous expansion of China's mushroom industry, people, to the continuous increase of edible mushrooms demand, make the output of bacterium chaff also increase thereupon.If bacterium chaff arbitrarily abandons or burns, easily cause environmental pollution and cause wasting of resources phenomenon.Therefore, the rational exploitation and utilization of bacterium chaff is most important with minimizing environmental pollution to cellulose resource recycle.Bacterium chaff is formed primarily of robust fibre, crude protein and crude fat.In addition, also containing mineral elements such as abundant amino acid and Ca.Current bacterium chaff is often used as matrix, fertilizer and feed etc.But edible fungi residue feed is not also utilized by spread, reason is that the crude fiber content in bacterium chaff is higher, is difficult to degraded.Only have and degraded further by the coarse fiber content in bacterium chaff, bacterium chaff could be used as animal-feed.Utilize the coarse fiber content in mix bacterium agent degradation bacteria chaff, edible fungi residue feed can be made more easily by animal absorption and digestion.
Summary of the invention
In order to solve above-mentioned technical problem, an object of the present invention is to provide mix bacterium agent of a kind of effective degradation bacteria chaff and preparation method thereof, another object of the present invention is to provide the application of above-mentioned mix bacterium agent.The present invention utilizes microbiological deterioration bacterium chaff nontoxic, simple to operate, and bacterium chaff degradation effect is obvious, and can not bring potential safety hazard to animal, economic benefit is obvious.Degradation process can not affect edible fungi residue feed mouthfeel, and what is more important degradation process belongs to sealing and fermenting, can not cause environmental pollution, be beneficial to environment protection.
In order to realize first above-mentioned object, present invention employs following technical scheme:
A kind of mix bacterium agent of effective degradation bacteria chaff, this mix bacterium agent is mixed with mix bacterium agent stoste with 1:5 ~ 5:1 ratio respectively by stalk fermentation bacterium liquid and lignocellulose degradation special bacterium liquid, then be inoculated in sterile distilled water with the mixing stoste of 0.25% ~ 1.0%, add the mixed fermentation liquid that 1.0 ~ 5.0% sterile distilled water weight are made up of aspergillus niger, mould simultaneously and stablize mixed culture 2 ~ 3d, obtain;
The preparation method of described mixed fermentation liquid is as follows: Aspergillus niger strain, mould are linked into respectively after PDA slant medium cultivates 3-4d, are washed down by the spore on inclined-plane, make spore suspension, viable count 106CFU/mL with sterilized water; Aspergillus niger, mould are obtained mixed fermentation liquid according to after the proportional liquid fermentation culture 7 ~ 8d of 15% ~ 25%;
Described stalk fermentation bacterium solution preparation method is as follows: first configure substratum, substratum formula for raw stock is: brown sugar 100g, sterilized water 1000mL; Add the loading of 5g stalk fermentation bacterium containing in the plastic tank of 1000mL substratum, sealing, can obtain stalk fermentation bacterium liquid after anaerobically fermenting 3d souring under 35 DEG C of conditions;
Described lignocellulose degradation special bacterium liquid and preparation method thereof is as follows: get 0.5g lignocellulose degradation special bacteria agent, adds in the plastic tank containing 1000mL distilled water, sealing, namely prepares lignocellulose degradation special bacterium liquid after cultivating 10h in 28 DEG C.
The preparation method of above-mentioned mix bacterium agent, the method comprises the following steps:
1), after Aspergillus niger strain, mould being linked into respectively PDA slant medium cultivating 3-4d, with sterilized water, the spore on inclined-plane is washed down, make spore suspension, viable count 10
6cFU/mL; Aspergillus niger, mould are obtained mixed fermentation liquid according to after the proportional liquid fermentation culture 7 ~ 8d of 15% ~ 25%;
2) first configure substratum, substratum formula for raw stock is: brown sugar 100g, sterilized water 1000mL; Add the loading of 5g stalk fermentation bacterium containing in the plastic tank of 1000mL substratum, sealing, can obtain stalk fermentation bacterium liquid after anaerobically fermenting 3d souring under 35 DEG C of conditions;
3) get 0.5g lignocellulose degradation special bacteria agent, add in the plastic tank containing 1000mL distilled water, sealing, after cultivating 10h in 28 DEG C, namely prepare lignocellulose degradation special bacterium liquid;
4) stalk fermentation bacterium liquid and lignocellulose degradation special bacterium liquid are mixed with mix bacterium agent stoste with 1:5 ~ 5:1 ratio respectively, then be inoculated in sterile distilled water with the mixing stoste of 0.25% ~ 1.0%, add the mixed fermentation liquid that 1.0 ~ 5.0% sterile distilled water weight are made up of aspergillus niger, mould simultaneously and stablize mixed culture 2 ~ 3d, obtain mix bacterium agent.
In order to realize second above-mentioned object, present invention employs following technical scheme:
Above-mentioned mix bacterium agent is used for the application of degradation bacteria chaff.
Mix bacterium agent degradation bacteria chaff of the present invention is easy to operate, and degradation effect is obvious.Adopt the bacterium chaff of mix bacterium agent degraded, crude fiber content reduces, and makes edible fungi residue feed be easier to animal digestion and absorption.In addition, the application of edible fungi residue feed can reduce abandoning of bacterium chaff and burn, and reduces environmental pollution, brings very large benefit to efforts at environmental protection.
Embodiment
1), after Aspergillus niger strain, mould (all purchasing Yiyuan Kangyuan Bio-Technology Co., Ltd.) being linked into respectively and PDA slant medium cultivates 3d, with sterilized water, the spore on inclined-plane is washed down, make spore suspension, viable count 10
6cFU/mL; Aspergillus niger, mould are obtained mixed fermentation liquid according to after the proportional liquid fermentation culture 7d of 20%;
2) first configure substratum, substratum formula for raw stock is: brown sugar 100g, sterilized water 1000mL; Add 5g stover ferment agent (buying agriculture Citroen zx bio tech ltd) to load in the plastic tank containing 1000mL substratum, sealing, can obtain stalk fermentation bacterium liquid after anaerobically fermenting 3d souring under 35 DEG C of conditions;
3) get 0.5g lignocellulose degradation special bacteria agent (buying Guangzhou Nongguan Biotech Co., Ltd.), add in the plastic tank containing 1000mL distilled water, sealing, after cultivating 10h in 28 DEG C, namely prepare lignocellulose degradation special bacterium liquid;
4) stalk fermentation bacterium liquid and lignocellulose degradation special bacterium liquid are mixed with mix bacterium agent stoste with 1:3 ratio respectively, then be inoculated in sterile distilled water with the mixing stoste of 0.25% ~ 1.0%, add the mixed fermentation liquid that 5.0% sterile distilled water weight is made up of aspergillus niger, mould simultaneously and stablize mixed culture 2d, obtain the mix bacterium agent of effective degradation bacteria chaff.
Utilize composite zymocyte liquid prepared by stalk fermentation bacterium liquid, lignocellulose degradation special bacteria and different ratios respectively, the ratio of spraying 1kg bacterium chaff according to every 10mL microbial inoculum is sprayed, and 24h sprays once, overturning 1 time, after cultivating 3d at 35 DEG C, measure the degradation rate of bacterium chaff.
Through the process of different microbial inoculum, flat mushroom bacterium chaff and Pleurotus eryngii bacterium chaff are degraded with different ratios.When result shows to utilize lignocellulose degradation special bacteria degraded flat mushroom bacterium chaff 3d, flat mushroom bacterium chaff degradation rate maximum value can reach 19.8%; Utilize mix bacterium agent (stover ferment agent and lignocellulose degradation special bacteria blending ratio are 4:2) degrade Pleurotus eryngii bacterium chaff 3d time, Pleurotus eryngii bacterium chaff degradation rate maximum value can be made to reach 13.7%.After the common degradation bacteria chaff 3d of the mixed fermentation liquid utilizing aspergillus niger, mould to form and mix bacterium agent (stover ferment agent and lignocellulose degradation special bacteria blending ratio are 4:2), flat mushroom bacterium chaff and Pleurotus eryngii bacterium chaff degradation rate reach 30.2% and 23.2% respectively, are 1.8 times, 1.7 times of the degradation rate before not adding aspergillus niger and mould fermented liquid respectively.Therefore aspergillus niger, mould mix common degradation bacteria chaff with straw degradative microbial inoculum etc., bacterium chaff degradation effect is best.Utilize aspergillus niger, mould and straw degradative microbial inoculum etc. to mix common degradation bacteria chaff not only simple and convenient, feasibility is higher.In addition, utilize different bacterium degraded flat mushroom bacterium chaff and Pleurotus eryngii bacterium chaff under the same terms, the degradation rate of flat mushroom bacterium chaff is all greater than the degradation rate of Pleurotus eryngii bacterium chaff.Therefore, flat mushroom bacterium chaff is easier to degraded compared to Pleurotus eryngii bacterium chaff, and namely flat mushroom bacterium chaff is more suitable for for animal-feed.
Claims (3)
1. the mix bacterium agent of an effective degradation bacteria chaff, it is characterized in that this mix bacterium agent is mixed with mix bacterium agent stoste with 1:5 ~ 5:1 ratio respectively by stalk fermentation bacterium liquid and lignocellulose degradation special bacterium liquid, then be inoculated in sterile distilled water with the mixing stoste of 0.25% ~ 1.0%, add the mixed fermentation liquid that 1.0 ~ 5.0% sterile distilled water weight are made up of aspergillus niger, mould simultaneously and stablize mixed culture 2 ~ 3d, obtain;
The preparation method of described mixed fermentation liquid is as follows: Aspergillus niger strain, mould are linked into respectively after PDA slant medium cultivates 3-4d, are washed down by the spore on inclined-plane, make spore suspension, viable count 10 with sterilized water
6cFU/mL; Aspergillus niger, mould are obtained mixed fermentation liquid according to after the proportional liquid fermentation culture 7 ~ 8d of 15% ~ 25%;
Described stalk fermentation bacterium solution preparation method is as follows: first configure substratum, substratum formula for raw stock is: brown sugar 100g, sterilized water 1000mL; Add the loading of 5g stalk fermentation bacterium containing in the plastic tank of 1000mL substratum, sealing, can obtain stalk fermentation bacterium liquid after anaerobically fermenting 3d souring under 35 DEG C of conditions;
Described lignocellulose degradation special bacterium liquid and preparation method thereof is as follows: get 0.5g lignocellulose degradation special bacteria agent, adds in the plastic tank containing 1000mL distilled water, sealing, namely prepares lignocellulose degradation special bacterium liquid after cultivating 10h in 28 DEG C.
2. the preparation method of mix bacterium agent according to claim 1, is characterized in that the method comprises the following steps:
1), after Aspergillus niger strain, mould being linked into respectively PDA slant medium cultivating 3-4d, with sterilized water, the spore on inclined-plane is washed down, make spore suspension, viable count 10
6cFU/mL; Aspergillus niger, mould are obtained mixed fermentation liquid according to after the proportional liquid fermentation culture 7 ~ 8d of 15% ~ 25%;
2) first configure substratum, substratum formula for raw stock is: brown sugar 100g, sterilized water 1000mL; Add the loading of 5g stalk fermentation bacterium containing in the plastic tank of 1000mL substratum, sealing, can obtain stalk fermentation bacterium liquid after anaerobically fermenting 3d souring under 35 DEG C of conditions;
3) get 0.5g lignocellulose degradation special bacteria agent, add in the plastic tank containing 1000mL distilled water, sealing, after cultivating 10h in 28 DEG C, namely prepare lignocellulose degradation special bacterium liquid;
4) stalk fermentation bacterium liquid and lignocellulose degradation special bacterium liquid are mixed with mix bacterium agent stoste with 1:5 ~ 5:1 ratio respectively, then be inoculated in sterile distilled water with the mixing stoste of 0.25% ~ 1.0%, add the mixed fermentation liquid that 1.0 ~ 5.0% sterile distilled water weight are made up of aspergillus niger, mould simultaneously and stablize mixed culture 2 ~ 3d, obtain mix bacterium agent.
3. mix bacterium agent according to claim 1 is used for the application of degradation bacteria chaff.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105859339A (en) * | 2016-03-30 | 2016-08-17 | 王云飞 | Flora for recycling treatment of organic wastes and application of flora |
| CN107494986A (en) * | 2017-09-28 | 2017-12-22 | 合肥福泉现代农业科技有限公司 | A kind of preparation method for the compound microorganism bacterium powder that chicken feed is done for edible mushroom bran of fermenting |
| CN114752592A (en) * | 2022-05-12 | 2022-07-15 | 浙江师范大学 | Microbial inoculum for fungus bran feed and preparation method thereof |
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| WO2008078878A1 (en) * | 2006-12-22 | 2008-07-03 | Deuk-Sik Lee | Fermented feeds for livestock farming using lactic acid bacteria and yeast and processing method thereof |
| CN103549129A (en) * | 2013-10-21 | 2014-02-05 | 中国农业科学院饲料研究所 | Enzyme and bacterium complexing agent for degrading crop straws |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| WO2008078878A1 (en) * | 2006-12-22 | 2008-07-03 | Deuk-Sik Lee | Fermented feeds for livestock farming using lactic acid bacteria and yeast and processing method thereof |
| CN103549129A (en) * | 2013-10-21 | 2014-02-05 | 中国农业科学院饲料研究所 | Enzyme and bacterium complexing agent for degrading crop straws |
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| CHINEDU S.N. ET AL.: "CELLULASE PRODUCTION BY WILD STRAINS OF ASPERGILLUS NIGER,PENICILLIUM CHRYSOGENUM AND TRICHODERMA HARZIANUM GROWN ON WASTE CELLULOSIC MATERIALS.", 《IFE JOURNAL OF SCIENCE》 * |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105859339A (en) * | 2016-03-30 | 2016-08-17 | 王云飞 | Flora for recycling treatment of organic wastes and application of flora |
| CN107494986A (en) * | 2017-09-28 | 2017-12-22 | 合肥福泉现代农业科技有限公司 | A kind of preparation method for the compound microorganism bacterium powder that chicken feed is done for edible mushroom bran of fermenting |
| CN114752592A (en) * | 2022-05-12 | 2022-07-15 | 浙江师范大学 | Microbial inoculum for fungus bran feed and preparation method thereof |
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