CN105087398B - A kind of mix bacterium agent and its preparation method and application of effective degradation mushroom bran - Google Patents
A kind of mix bacterium agent and its preparation method and application of effective degradation mushroom bran Download PDFInfo
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- CN105087398B CN105087398B CN201510569108.7A CN201510569108A CN105087398B CN 105087398 B CN105087398 B CN 105087398B CN 201510569108 A CN201510569108 A CN 201510569108A CN 105087398 B CN105087398 B CN 105087398B
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- mushroom bran
- bacterium
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- 241000894006 Bacteria Species 0.000 title claims abstract description 60
- 235000001674 Agaricus brunnescens Nutrition 0.000 title claims abstract description 49
- 238000006731 degradation reaction Methods 0.000 title claims abstract description 46
- 230000015556 catabolic process Effects 0.000 title claims abstract description 44
- 238000002360 preparation method Methods 0.000 title claims abstract description 12
- 238000000855 fermentation Methods 0.000 claims abstract description 33
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 31
- 230000004151 fermentation Effects 0.000 claims abstract description 30
- 239000000203 mixture Substances 0.000 claims abstract description 26
- 239000007788 liquid Substances 0.000 claims abstract description 24
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 23
- 241000228245 Aspergillus niger Species 0.000 claims abstract description 17
- 239000012153 distilled water Substances 0.000 claims abstract description 14
- 239000008223 sterile water Substances 0.000 claims abstract description 9
- 239000000725 suspension Substances 0.000 claims abstract description 5
- 239000001963 growth medium Substances 0.000 claims description 11
- 239000004033 plastic Substances 0.000 claims description 8
- 238000007789 sealing Methods 0.000 claims description 8
- 238000000034 method Methods 0.000 claims description 2
- 239000007921 spray Substances 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 4
- 235000001603 Pleurotus ostreatus Nutrition 0.000 description 8
- 244000252132 Pleurotus eryngii Species 0.000 description 7
- 235000001681 Pleurotus eryngii Nutrition 0.000 description 7
- 240000001462 Pleurotus ostreatus Species 0.000 description 7
- 241000233866 Fungi Species 0.000 description 6
- 239000000835 fiber Substances 0.000 description 6
- 241001465754 Metazoa Species 0.000 description 5
- 239000002068 microbial inoculum Substances 0.000 description 5
- 230000000593 degrading effect Effects 0.000 description 3
- 238000003912 environmental pollution Methods 0.000 description 3
- 238000010521 absorption reaction Methods 0.000 description 2
- 229920002678 cellulose Polymers 0.000 description 2
- 239000001913 cellulose Substances 0.000 description 2
- 230000003413 degradative effect Effects 0.000 description 2
- 230000029087 digestion Effects 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 239000010907 stover Substances 0.000 description 2
- 239000010902 straw Substances 0.000 description 2
- 229920000742 Cotton Polymers 0.000 description 1
- 241000237504 Crassostrea virginica Species 0.000 description 1
- 235000019750 Crude protein Nutrition 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 230000008953 bacterial degradation Effects 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 235000019784 crude fat Nutrition 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000003337 fertilizer Substances 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
- 239000002023 wood Substances 0.000 description 1
Classifications
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Fodder In General (AREA)
Abstract
The present invention relates to a kind of mix bacterium agents of effective degradation mushroom bran, and the mix bacterium agent is by stalk fermentation bacterium solution and the special bacterium solution of lignocellulose degradation respectively with 1:5~5:1 ratio is mixed with mix bacterium agent stoste, is then inoculated in sterile distilled water with 0.25% ~ 1.0% mixing stoste, while 1.0 ~ 5.0% sterile distilled water weight are added and stablize 2 ~ 3d of mixed culture by the mixed fermentation liquid that aspergillus niger, mould form, and obtains；The preparation method of mixed fermentation liquid is as follows：Aspergillus niger strain, mould are respectively connected to after cultivating 34 d on PDA slant mediums, the spore on inclined-plane is washed down with sterile water, spore suspension, viable count 10 is made6 CFU/mL；By aspergillus niger, mould according to obtaining mixed fermentation liquid after 15% ~ 25% 7 ~ 8d of proportional liquid fermented and cultured；The mix bacterium agent degradation mushroom bran of the present invention is easy to operate, and degradation effect is apparent.
Description
Technical field
The present invention relates to the degradation field of edible mushroom trade waste more particularly to a kind of mixing of effective degradation mushroom bran
Microbial inoculum and its preparation method and application.
Background technology
Mushroom bran is called bacteria residue, refers to generated culture medium discarded object after culturing edible fungus, includes mainly cotton seed hull, wood
The cellulose components such as bits and stalk.In recent years, with the continuous expansion of China's mushroom industry, people are to edible mushroom demand
It is continuously increased so that the yield of mushroom bran is consequently increased.If mushroom bran is arbitrarily abandoned or is burnt, be easy to cause environmental pollution and
Cause wasting of resources phenomenon.Therefore, the rational exploitation and utilization of mushroom bran cellulose resource is recycled and is reduced environmental pollution to
It closes important.Mushroom bran is mainly made of crude fibre, crude protein and crude fat.In addition, also containing the minerals such as abundant amino acid and Ca
Element.Mushroom bran is often used as matrix, fertilizer and feed etc. at present.But edible fungi residue feed is not widely applied utilization also, and reason exists
Crude fiber content in mushroom bran is higher, it is difficult to degrade.Only the coarse fiber content in mushroom bran is further degraded, mushroom bran ability
As animal feed.Coarse fiber content in mushroom bran of being degraded using mix bacterium agent, can be such that edible fungi residue feed is more easy to by animal digestion
And absorption.
Invention content
In order to solve the above technical problems, it is an object of the present invention to provide a kind of Mixed Microbes of effective degradation mushroom bran
Agent and preparation method thereof, another object of the present invention are to provide the application of above-mentioned mix bacterium agent.The present invention utilizes micro- life
Object is degraded, and mushroom bran is nontoxic, and easy to operate, mushroom bran degradation effect is apparent, and security risk will not be carried out to lead for animal, and economic benefit is apparent.
Degradation process does not interfere with edible fungi residue feed mouthfeel, and what is more important degradation process belongs to sealing and fermenting, and environment will not be caused dirty
Dye is conducive to environmental protection.
In order to realize first above-mentioned purpose, present invention employs technical solutions below：
A kind of mix bacterium agent of effective degradation mushroom bran, the mix bacterium agent is by stalk fermentation bacterium solution and lignocellulose degradation special bacterium
Liquid is respectively with 1:5~5:1 ratio is mixed with mix bacterium agent stoste, is then inoculated in 0.25% ~ 1.0% mixing stoste sterile
In distilled water, while 1.0 ~ 5.0% sterile distilled water weight is added, mixing is stablized by the mixed fermentation liquid that aspergillus niger, mould form
2 ~ 3 d are cultivated, are obtained；
The preparation method of the mixed fermentation liquid is as follows：Aspergillus niger strain, mould are respectively connected to PDA inclined-plane cultures
After cultivating 3-4 d on base, the spore on inclined-plane is washed down with sterile water, spore suspension, viable count 10 is made6CFU/mL；It will
Aspergillus niger, mould after 15% ~ 25% 7 ~ 8 d of proportional liquid fermented and cultured according to obtaining mixed fermentation liquid；
The stalk fermentation bacterium solution preparation method is as follows：Culture medium is first configured, culture medium formula for raw stock is：Brown sugar
100 g, 1000 mL of sterile water；5 g stalk fermentation bacterium are added to be packed into the plastic barrel containing 1000 mL culture mediums, sealing, 35
Under the conditions of DEG C stalk fermentation bacterium solution is can be obtained after 3 d souring of anaerobic fermentation；
The lignocellulose degradation special bacterium liquid and preparation method thereof is as follows：0.5 g lignocellulose degradation special bacteria agents are taken, are added to
In plastic barrel containing 1000 mL distilled water, the special bacterium solution of lignocellulose degradation is prepared after cultivating 10 h in 28 DEG C in sealing.
The preparation method of above-mentioned mix bacterium agent, this method include the following steps：
1）Aspergillus niger strain, mould are respectively connected to after cultivating 3-4 d on PDA slant mediums, it will be oblique with sterile water
Spore on face is washed down, and spore suspension, viable count 10 is made6CFU/mL；By aspergillus niger, mould according to 15% ~ 25% ratio
Mixed fermentation liquid is obtained after 7 ~ 8 d of liquid fermentation and culture；
2）Culture medium is first configured, culture medium formula for raw stock is：100 g of brown sugar, 1000 mL of sterile water；5 g stalks are added
Zymophyte is packed into the plastic barrel containing 1000 mL culture mediums, and sealing can obtain under the conditions of 35 DEG C after 3 d souring of anaerobic fermentation
To stalk fermentation bacterium solution；
3）0.5 g lignocellulose degradation special bacteria agents are taken, are added in the plastic barrel containing 1000 mL distilled water, are sealed, in 28
DEG C culture 10 h after i.e. the special bacterium solution of lignocellulose degradation is prepared；
4）Stalk fermentation bacterium solution is with the special bacterium solution of lignocellulose degradation respectively with 1:5~5:1 ratio is mixed with mix bacterium agent original
Then liquid is inoculated in 0.25% ~ 1.0% mixing stoste in sterile distilled water, while 1.0 ~ 5.0% sterile distilled water weights are added
Amount stablizes 2 ~ 3 d of mixed culture by the mixed fermentation liquid that aspergillus niger, mould form, and obtains mix bacterium agent.
In order to realize second above-mentioned purpose, present invention employs technical solutions below：
Application of the above-mentioned mix bacterium agent for mushroom bran of degrading.
The mix bacterium agent degradation mushroom bran of the present invention is easy to operate, and degradation effect is apparent.The bacterium degraded using mix bacterium agent
Chaff, crude fiber content are reduced, and edible fungi residue feed is made to be easier to animal digestion and absorption.In addition, the application of edible fungi residue feed can be reduced
The discarding and burning of mushroom bran, reduce environmental pollution, and prodigious benefit is brought to efforts at environmental protection.
Specific implementation mode
1）By Aspergillus niger strain, mould（Purchase Yiyuan Kangyuan Bio-Technology Co., Ltd.）It is respectively connected to the inclined-planes PDA
After cultivating 3 d on culture medium, the spore on inclined-plane is washed down with sterile water, spore suspension, viable count 10 is made6CFU/mL；
By aspergillus niger, mould according to obtaining mixed fermentation liquid after 20% 7 d of proportional liquid fermented and cultured；
2）Culture medium is first configured, culture medium formula for raw stock is：100 g of brown sugar, 1000 mL of sterile water；5 g stalks are added
Leavening (buying agriculture Citroen zx bio tech ltd) is packed into the plastic barrel containing 1000 mL culture mediums, sealing, in 35 DEG C of items
Under part stalk fermentation bacterium solution is can be obtained after 3 d souring of anaerobic fermentation；
3）0.5 g lignocellulose degradations special bacteria agent (buying Guangzhou Nongguan Biotech Co., Ltd.) is taken, is added to and contains
In the plastic barrel of 1000 mL distilled water, the special bacterium solution of lignocellulose degradation is prepared after cultivating 10 h in 28 DEG C in sealing；
4）Stalk fermentation bacterium solution is with the special bacterium solution of lignocellulose degradation respectively with 1:3 ratios are mixed with mix bacterium agent stoste,
Then it is inoculated in sterile distilled water with 0.25% ~ 1.0% mixing stoste, while 5.0% sterile distilled water weight is added by black song
Mould, mould composition mixed fermentation liquid stablizes 2 d of mixed culture, obtains the mix bacterium agent of effectively degradation mushroom bran.
It is utilized respectively composite zymocyte prepared by stalk fermentation bacterium solution, lignocellulose degradation special bacteria and different proportion
Liquid, the ratio that 1 kg mushroom brans are sprayed according to every 10 mL microbial inoculums are sprayed, and 24 h sprinklings are primary, and 3 d are cultivated at overturning 1 time, 35 DEG C
Afterwards, the degradation rate of mushroom bran is measured.
Processing through different microbial inoculums, oyster mushroom mushroom bran and Pleurotus eryngii mushroom bran are degraded in different proportions.The result shows that using thick
When fiber degradation special bacteria degradation oyster mushroom 3 d of mushroom bran, oyster mushroom mushroom bran degradation rate maximum value is up to 19.8%；Utilize mix bacterium agent
（Stover ferment agent is 4 with lignocellulose degradation special bacteria mixed proportion:2）When degradation Pleurotus eryngii 3 d of mushroom bran, Pleurotus eryngii can be made
Mushroom bran degradation rate maximum value is up to 13.7%.The mixed fermentation liquid and mix bacterium agent formed using aspergillus niger, mould（Stover ferment agent
It is 4 with lignocellulose degradation special bacteria mixed proportion:2）After 3 d of common degradation mushroom bran, oyster mushroom mushroom bran and the degradation of Pleurotus eryngii mushroom bran
Rate respectively reaches 30.2% and 23.2%, is be not added with the degradation rate before aspergillus niger and mould zymotic fluid 1.8 times, 1.7 respectively
Times.Therefore aspergillus niger, mould and straw degradative microbial inoculum etc. mix mushroom bran of degrading jointly, mushroom bran degradation effect is best.Using aspergillus niger,
Mould and straw degradative microbial inoculum etc. mix that mushroom bran of degrading jointly is not only simple and convenient, and feasibility is higher.In addition, sharp under the same terms
It is all higher than the degradation rate of Pleurotus eryngii mushroom bran with different bacterium degradation oyster mushroom mushroom bran and Pleurotus eryngii mushroom bran, the degradation rate of oyster mushroom mushroom bran.Cause
This, oyster mushroom mushroom bran is easier to degrade compared to Pleurotus eryngii mushroom bran, i.e. oyster mushroom mushroom bran is particularly suited for animal feed.
Claims (1)
1. a kind of method of degradation mushroom bran, which is characterized in that the ratio that every 10 mL mix bacterium agents spray 1 kg mushroom brans is sprayed, and 24
H sprinklings are primary, and 3 d are cultivated at overturning 1 time, 35 DEG C；
The mix bacterium agent is by stalk fermentation bacterium solution and the special bacterium solution of lignocellulose degradation respectively with 1:5~5:1 ratio is mixed with mixed
Then combined bacteria agent stoste is inoculated in 0.25% ~ 1.0% mix bacterium agent stoste in sterile distilled water, while being added 1.0 ~ 5.0%
Sterile distilled water weight stablizes 2 ~ 3d of mixed culture by the mixed fermentation liquid that aspergillus niger, mould form, and obtains；
The preparation method of the mixed fermentation liquid is as follows：Aspergillus niger strain, mould are respectively connected on PDA slant mediums
After cultivating 3-4d, the spore on inclined-plane is washed down with sterile water, spore suspension, viable count 10 is made6CFU/mL；By black song
Mould, mould after 15% ~ 25% 7 ~ 8d of proportional liquid fermented and cultured according to obtaining mixed fermentation liquid；
The stalk fermentation bacterium solution preparation method is as follows：Culture medium is first configured, culture medium formula for raw stock is：Brown sugar 100g,
Sterile water 1000mL；5g stalk fermentation bacterium are added to be packed into the plastic barrel of the culture medium containing 1000mL, sealing is detested under the conditions of 35 DEG C
Stalk fermentation bacterium solution is can be obtained after aerobe fermentation 3d souring；
The lignocellulose degradation special bacterium liquid and preparation method thereof is as follows：0.5g lignocellulose degradation special bacteria agents are taken, is added to and contains
In the plastic barrel of 1000mL distilled water, the special bacterium solution of lignocellulose degradation is prepared after cultivating 10h in 28 DEG C in sealing.
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| CN105859339A (en) * | 2016-03-30 | 2016-08-17 | 王云飞 | Flora for recycling treatment of organic wastes and application of flora |
| CN107494986A (en) * | 2017-09-28 | 2017-12-22 | 合肥福泉现代农业科技有限公司 | A kind of preparation method for the compound microorganism bacterium powder that chicken feed is done for edible mushroom bran of fermenting |
| CN114752592A (en) * | 2022-05-12 | 2022-07-15 | 浙江师范大学 | Microbial inoculum for fungus bran feed and preparation method thereof |
Citations (1)
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| CN103549129A (en) * | 2013-10-21 | 2014-02-05 | 中国农业科学院饲料研究所 | Enzyme and bacterium complexing agent for degrading crop straws |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103549129A (en) * | 2013-10-21 | 2014-02-05 | 中国农业科学院饲料研究所 | Enzyme and bacterium complexing agent for degrading crop straws |
Non-Patent Citations (4)
| Title |
|---|
| CELLULASE PRODUCTION BY WILD STRAINS OF ASPERGILLUS NIGER,PENICILLIUM CHRYSOGENUM AND TRICHODERMA HARZIANUM GROWN ON WASTE CELLULOSIC MATERIALS.;Chinedu S.N. et al.;《Ife Journal of Science》;20111231;第13卷(第1期);第57-62页 * |
| 平菇和杏鲍菇两种菌糠的营养价值分析;张丽影等;《广东微量元素科学》;20150531;第22卷(第5期);第24-28页，尤其是第25页材料与方法，第26页结果与分析2.3,第27页结论 * |
| 玉米秸秆干式厌氧发酵乙酸产生规律及其对发酵的影响;陈斌等;《环境科学研究》;20150430;第28卷(第4期);第647-652页，尤其是第648页材料与方法 * |
| 生物秸秆发酵剂的使用方法;郝增锁;《农业科技通讯》;20001031;第30页下半部分中间第2段 * |
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