CN105076226A - Plant tissue disinfectant - Google Patents
Plant tissue disinfectant Download PDFInfo
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- CN105076226A CN105076226A CN201510539505.XA CN201510539505A CN105076226A CN 105076226 A CN105076226 A CN 105076226A CN 201510539505 A CN201510539505 A CN 201510539505A CN 105076226 A CN105076226 A CN 105076226A
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- disinfectant
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Abstract
The invention discloses a plant tissue disinfectant. The plant tissue disinfectant is characterized by containing the ingredients in parts by weight: 10-23 parts of KMnO4, 5-9 parts of sodium alkylarylsulfonate, 14-26 parts of trisodium phosphate, 11-17 parts of sodium silicate, 1-7 parts of carboxyl methylcellulose, 8-13 parts of tallow fatty acid sodium sulfate, 7-13 parts of ether sodium sulfate, 9-24 parts of fatty alcohol polyoxyethylene, 1-2 parts of lonicera japonica extract and 0.002-0.006 part of chitosan. According to the disinfectant disclosed by the invention, a chemical disinfection and biological disinfection combined formula is adopted, all the ingredients are in synergism, and the disinfection effect is more remarkable, so that the disinfectant is applicable to the disinfection of a variety of plant seeds and plant tissue; and a preparation method is simple and easy.
Description
Technical field
The present invention relates to a kind of plant tissue disinfectant.
Background technology
In Plant Tissue Breeding and gene engineering research, to explant disinfection, be very important previous work, the kind of explant is more, and wherein, seed is the class be even more important.In the research of molecular biotechnology, aseptic seedling is desirable test starting material, often needs to cultivate aseptic seedling.In tissue training, using aseptic seedling as explant, callus and regeneration plant can be obtained, carry out transgeneic procedure further, in high-quality RNA extracts, also will use aseptic seedling.
The plant seed disinfectant used of sterilizing has multiple, and common are hypochlorite, hydrogen peroxide, bromine water, mercury chloride, alcohol etc., offset the research of chemical agent decontamination effect, forefathers have done a large amount of work.
Shitosan (chitosan) is that the chitin (chitin) extensively existed by natural world obtains through deacetylation, chemical name is Chitosan (1-4)-2-amino-B-D glucose, from 1859, after first Frenchman Rouget obtains shitosan, the biological functionality of this natural polymer and compatibility, blood compatibility, safety, the premium properties such as microbic resolvability are by all trades and professions extensive concern, at medicine, food, chemical industry, cosmetics, water treatment, METAL EXTRACTION and recovery, the application study of the numerous areas such as biochemistry and biomedical engineering achieves major progress.Shitosan is easy to dissolve in weak acid solvent, it is worthy of note that these amino are by carrying out anti-bacteria in conjunction with negatron containing amino (NH2+) in the solution after dissolving especially.
Summary of the invention
Technical problem to be solved by this invention is to provide a kind of plant seed disinfectant, particularly a kind of environmental protection, efficiently plant seed biological disinfectant.
For solving the problems of the technologies described above, the technical solution used in the present invention is:
A kind of plant tissue disinfectant, is characterized in that: containing KMnO
410-23 part, sodium alklyarylsulfonate 5-9 part, tertiary sodium phosphate 14-26 part, sodium silicate 11-17 part, carboxyl methylcellulose 1-7 part, tallow acid sodium sulphate 8-13 part, ether sodium sulfate 7-13 part, aliphatic alcohol polyethenoxy 9-24 part, Flos Lonicerae extractive solution 1-2 part, shitosan 0.002-0.006.
The invention has the beneficial effects as follows: the formula that this disinfectant adopts chemical disinfection and biological antiseptic to combine, wherein each composition synergy, Disinfection Effect is more remarkable, and be applicable to the sterilization of various plant seed and plant tissue, compound method is simple and easy to get.
Embodiment
Embodiment 1
A kind of plant tissue disinfectant, is characterized in that: containing KMnO
410-23 part, sodium alklyarylsulfonate 5-9 part, tertiary sodium phosphate 14-26 part, sodium silicate 11-17 part, carboxyl methylcellulose 1-7 part, tallow acid sodium sulphate 8-13 part, ether sodium sulfate 7-13 part, aliphatic alcohol polyethenoxy 9-24 part, Flos Lonicerae extractive solution 1-2 part, shitosan 0.002-0.006.
Claims (1)
1. a plant tissue disinfectant, is characterized in that: containing KMnO
410-23 part, sodium alklyarylsulfonate 5-9 part, tertiary sodium phosphate 14-26 part, sodium silicate 11-17 part, carboxyl methylcellulose 1-7 part, tallow acid sodium sulphate 8-13 part, ether sodium sulfate 7-13 part, aliphatic alcohol polyethenoxy 9-24 part, Flos Lonicerae extractive solution 1-2 part, shitosan 0.002-0.006.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510539505.XA CN105076226A (en) | 2015-08-30 | 2015-08-30 | Plant tissue disinfectant |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510539505.XA CN105076226A (en) | 2015-08-30 | 2015-08-30 | Plant tissue disinfectant |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105076226A true CN105076226A (en) | 2015-11-25 |
Family
ID=54558290
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510539505.XA Pending CN105076226A (en) | 2015-08-30 | 2015-08-30 | Plant tissue disinfectant |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105076226A (en) |
-
2015
- 2015-08-30 CN CN201510539505.XA patent/CN105076226A/en active Pending
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| WD01 | Invention patent application deemed withdrawn after publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20151125 |